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Pseudomonas Spp. ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺩﻳﻨﺎﻣﻴﺰﻡ ﺟﻤﻌﻴﺖ ﺑﺮﺧﻲ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣ

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Pseudomonas Spp. ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺩﻳﻨﺎﻣﻴﺰﻡ ﺟﻤﻌﻴﺖ ﺑﺮﺧﻲ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣ ﻧﻴﻚ ﻧﮋﺍﺩ ﻛﺎﻇﻢ ﭘﻮﺭ ﻭ ﻣﺎﻧﺴﻮ: ﻣﻄﺎﻟﻌﻪ ﺩﻳﻨﺎﻣﻴﺰﻡ ﺟﻤﻌﻴﺖ ﺑﺮﺧﻲ ﺟﺪﺍﻳﻪ ﻫﺎﻱ... ٢١٧ ﻣﺠﻠﻪ ﻋﻠﻮﻡ ﻛﺸﺎﻭﺭﺯﻱ ﺍﻳﺮﺍﻥ ﺟﻠﺪ ٣٥، ﺷﻤﺎﺭﻩ ١، ﺳﺎﻝ ١٣٨٣ (٢٢٦-٢١٧) ﻣﻄﺎﻟﻌﻪ ﺩﻳﻨﺎﻣﻴﺰﻡ ﺟﻤﻌﻴﺖ ﺑﺮﺧﻲ ﺟﺪﺍﻳﻪ ﻫﺎﻱ .Pseudomonas spp ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﻣﺨﺘﻠﻒ ﺭﻭﻱ ﺍﻧﺪﺍﻣﻬﺎﻱ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﻣﺼﻄﻔﻲ ﻧﻴﻚ ﻧﮋﺍﺩ ﻛﺎﻇﻢ ﭘﻮﺭ١ ﻭ ﭼﺎﻟﺰ ﻣﺎﻧﺴﻮ٢ ١، ﺍﺳﺘﺎﺩﻳﺎﺭ، ﺩﺍﻧﺸﻜﺪﻩ ﻛﺸﺎﻭﺭﺯﻱ ، ﺩﺍﻧﺸﮕﺎﻩ ﮔﻴﻼﻥ ٢، ﺍﺳﺘﺎﺩ ﺑﺎﻛﺘﺮﻳﻮﻟﻮﮊﻱ ﮔﻴﺎﻫﻲ ، ﺍﻳﻨﺮﺍ، ﺁﻧﮋﻩ – ﻓﺮﺍﻧﺴﻪ ﺗﺎﺭﻳﺦ ﭘﺬﻳﺮﺵ ﻣﻘﺎﻟﻪ ١٨/٤/٨٢ ﺧﻼﺻﻪ ﺩﺭ ﺍﻳﻦ ﺗﺤﻘﻴﻖ ﻣﻴﺰﺍﻥ ﺟﻤﻌﻴﺖ ﻭ ﻧﺤﻮﻩ ﺍﻧﺘﺸﺎﺭ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣﺨﺘﻠﻒ .Pseudomonas spp ﻛﻪ ﺍﺯ ﻧﻈﺮ ﮊﻧﺘﻴﻜﻲ ﺑﺎ ﻳﻜﺪﻳﮕﺮ ﻧﺰﺩﻳﻚ ﺑﻮﺩﻧﺪ ﺭﻭﻱ ﺍﻧﺪﺍﻣﻬﺎﻱ ﻣﺨﺘﻠﻒ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ (ﺭﻳﺸﻪ ، ﻃﻮﻗﻪ ، ﺳﺎﻗﻪ ، ﺑ ﺮﮒ ﻭ ﻣﺮﻳﺴﺘﻢ ﺍﻧﺘﻬﺎﻳﻲ ) ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﭘﺎﻳﻴﻦ (ﮔﻠﺨﺎﻧﻪ ) ﻭ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﺑﺎﻻ (ﺍﻃﺎﻗﻚ ﺭﺷﺪ ) ﻣﻮﺭﺩ ﺑﺮﺭﺳﻲ ﻗﺮﺍﺭ ﮔﺮﻓﺘﻨﺪ . ﻧﺘﺎﻳﺞ ﺗﺤﻘﻴﻖ ﻧﺸﺎﻥ ﺩﺍﺩ ﻛﻪ ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﺑﺎﻻ ﻣﻴﺰﺍﻥ ﻛﻠﻨﻴﺰﺍﺳﻴﻮﻥ ﺑﺎﻛﺘﺮﻱ ﺭﻭﻱ ﺗﻤﺎﻣﻲ ﺍﻧﺪﺍﻣﻬﺎﻱ ﮔﻴﺎﻩ ﺍﻓﺰﺍﻳﺶ ﻣﻲ ﻳﺎﺑﺪ ﻭ ﻋﻼﺋﻢ ﺑﻴﻤﺎﺭﻱ ﺧﺎﻝ ﺯﺩﮔﻲ ﺑ ﺎ ﺍﻧﺘﻘﺎﻝ ﻧﺸﺎ ﻫﺎﻱ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﺑﻪ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﺑﺎﻻ ﻇﺎﻫﺮ ﺷﺪ . ﻣﻨﻄﻘﻪ ﻣﺮﻳﺴﺘﻢ ﺍﻧﺘﻬﺎﻳﻲ ﻭ ﻃﻮﻗﻪ ﺑﻴﺶ ﺍﺯ ﺳﺎﻳﺮ ﺍﻧﺪﺍﻣﻬﺎﻱ ﮔﻴﺎﻩ ﺗﻮﺳﻂ ﺳﻠﻮﻟﻬﺎﻱ ﺑﺎﻛﺘﺮﻱ ﻛﻠﻨﻴﺰﻩ ﺷﺪ . ﻣﻴﺰﺍﻥ ﺗﻜﺜﻴﺮ ﺟﺪﺍﻳﻪ ﻫﺎﻳﻲ ﻣﻮﺗﺎﻥ hrp ﺩﺭ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﺑﺎﻻ ﺭﻭﻱ ﺗﻤﺎﻣﻲ ﺍﻧﺪﺍﻣﻬﺎﻱ ﮔﻴﺎﻫﻲ ﺑﻄﻮﺭ ﻣﻌﻨﻲ ﺩﺍﺭﻱ ﭘﺎﻳﻴﻦ ﺗﺮ ﺍﺯ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻭﺣﺸﻲ ﻣﺮﺑﻮﻃﻪ ﺑﻮﺩ . ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣﻮﺗﺎﻥ hrp ﺩﺭ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﭘﺎﻳﻴﻦ ﺗﻨﻬﺎ ﺩﺭ ﻣﻨﻄﻘﻪ ﻃﻮﻗﻪ ﺗﺸﺨﻴﺺ ﺩﺍﺩﻩ ﺷﺪﻧﺪ . ﻣﻴﺰﺍﻥ ﺟﻤﻌﻴﺖ ﺑﺎﻛﺘﺮﻳﻬﺎﻱ ﺳﺎﭘﺮﻭﻓﻴﺖ ﺩﺭ ﺷﺮﺍﻳﻂ ﮔﻠﺨﺎﻧﻪ ﻭ ﺍﻃﺎﻗﻚ ﺭﺷﺪ ﺗﻘﺮﻳﺒﺎ ﻣﺸﺎﺑﻪ ﺑﻮﺩ . ﺍﺯ ﻣﻴﺎﻥ ﺑﺎﻛﺘﺮﻳﻬﺎﻱ ﺳﺎﭘﺮﻭﻓﻴﺖ ﺑﻴﺸﺘﺮﻳﻦ ﺟﻤﻌﻴﺖ ﻣﺮﺑﻮﻁ ﺑﻪ Stenotrophomonas maltophilia ﺗﺸﺨﻴﺺ ﺩﺍﺩﻩ ﺷﺪ . ﺩﺭ ﻣﺠﻤﻮﻉ ﺍﻳﻨﻄﻮﺭ ﺍﺳﺘﻨﺒﺎﻁ ﻣﻲ ﺷﻮﺩ ﻛﻪ ﺑﺎ ﺍﻓﺰﺍﻳﺶ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ، ﻣﻴﺰﺍﻥ ﺗﻜﺜﻴﺮ ﺳﻠﻮﻟﻬﺎﻱ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﺗﻤﺎﻣﻲ ﺍﻧﺪﺍﻣﻬﺎﻱ ﮔﻴﺎﻩ ﺍﻓﺰﺍﻳﺶ ﻳﺎﻓﺘﻪ ﻭ ﺍﻳﻦ ﺍﻣﺮ ﻣﻨﺠﺮ ﺑﻪ ﺑﻮﺟﻮﺩ ﺁﻣﺪﻥ ﻋﻼﺋﻢ ﺑﻴﻤﺎﺭﻱ ﺩﺭ ﺑﺎﻓﺘﻬﺎﻱ ﺣﺴﺎﺱ ﮔﻴﺎﻩ ﻧﻈﻴﺮ ﺑﺮﮔﻬﺎ ﻭ ﻣﻴﻮﻩ ﻫﺎ ﮔﺮﺩﻳﺪ. ﻭﺍﮊﻩ ﻫﺎﻱ ﻛﻠﻴﺪﻱ : ﺑﻴﻤﺎﺭﻱ ﺧﺎﻝ ﺯﺩﮔﻲ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ، ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ،ﺍﻧﺘﺸﺎﺭ ﺑﺎﻛﺘﺮﻱ، .Pseudomonas spp ، Stenotrophomonas maltophilia ﻣﻘﺪﻣﻪ ﻓﻀﺎﻱ ﺯﻳﺮ ﺭﻭﺯﻧﻪ ﻫﺎ ﺗﻜﺜﻴﺮ ﻣﻲ ﻳﺎﺑﻨﺪ . ﺍﻳﻦ ﻣﺤﻠﻬﺎ ﻫﻤﭽﻨﻴﻦ ﺑﺎﻋﺚ ﺑﻴﻤﺎﺭﻱ ﺧﺎﻝ ﺯﺩﮔﻲ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﺩﺭ ﺍﺛﺮ Ptt ﻣﺤﺎﻓﻈﺖ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﻣﻘﺎﺑﻞ ﺧﺸﻜﻲ ﻣﻲ ﺷﻮﻧﺪ (١٦). ﺯﻧﺪﮔﻲ (Pseudomonas tomato pv.tomato) ﺑﺮﺍﻱ ﺍﻭﻟﻴﻦ ﺑﺎﺭ ﺩﺭ ﺍﭘﻲ ﻓﻴﺖ ﻋﻼﻭﻩ ﺑﺮ ﺍﻧﺪﺍﻣﻬﺎﻱ ﻫﻮﺍﻳﻲ ﺭﻭﻱ ﺭﻳﺸﻪ ﻭ ﺑﺬﺭ ﻧﻴﺰ ﮔﺰﺍﺭﺵ ﺗﺎﻳﻮﺍﻥ ﺗﻮﺳﻂ ﺍﻛﺎﺑﻪ (١٩٣٣) ﮔﺰﺍﺭﺵ ﺷﺪ . ﺩﺭ ﺍﻳﺮﺍﻥ ﺍﻳﻦ ﺑﻴﻤﺎﺭﻱ ﺷﺪﻩ ﺍﺳﺖ (١٧). ﻣﺎﺭﻳﺎﻧﻮ ﻭ ﻣﻚ ﻛﺎﺭﺗﺮ (١٩٩٢) ﮔﺰﺍﺭﺵ ﻛﺮﺩﻧﺪ ﺩﺭ ﻣﺰﺍﺭﻉ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﻭﺭﺍﻣﻴﻦ ﺗﻮﺳﻂ ﺷﻬﺮﻳﺎﺭﻱ ﻭ ﺭﺣﻴﻤﻴﺎﻥ ﻛﻪ Ptt ﻭ (P.syringae.pv.syrinae (Pss ﻗﺎﺩﺭﻧﺪ ﺑﺼﻮﺭﺕ ﮔﺰﺍﺭﺵ ﮔﺮﺩﻳﺪ (١). ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺑﺬﺭ ﺯﺍﺩ ﺑﻮﺩﻥ Ptt ﺍﻳﻦ ﺑﻴﻤﺎﺭﻱ ﺍﭘﻲ ﻓﻴﺖ ﺩﺭ ﺭﻳﺸﻪ ﻭ ﻗﺴﻤﺘﻬﺎﻱ ﻫﻮﺍﻳﻲ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﻭ ﺩﺍﻣﻨﻪ ﺍﺣﺘﻤﺎﻻ ﺩﺭ ﺍﻛﺜﺮ ﻧﻘﺎﻁ ﺟﻬﺎﻥ ﭘﺮﺍﻛﻨﺪﻩ ﺍﺳﺖ (١٢). ﺩﺭ ﭼﺮﺧﻪ ﻭﺳﻴﻌﻲ ﺍﺯ ﻋﻠﻔﻬﺎﻱ ﻫﺮﺯ ﺩﺍﺋﻤﻲ ﺩﺭ ﺷﺮﺍﻳﻂ ﺁﺏ ﻭ ﻫﻮﺍﻳﻲ ﻣﺨﺘﻠﻒ ﺑﻪ ﺯﻧﺪﮔﻲ ﺑﻴﻮﻟﻮﮊﻳﻚ ﺑﺎﻛﺘﺮﻱ Ptt ﺩﻭ ﻣﺮﺣﻠﻪ ﺑﻴﻤﺎﺭﻳﺰﺍﻳﻲ ﻭ ﺍﭘﻲ ﻓﻴﺖ ﻣﺪﺕ ﻃﻮﻻﻧﻲ ﺯﻧﺪﮔﻲ ﻧﻤﺎﻳﻨﺪ . ﺍﺳﺘﻔﺎﻧﻲ ﻭ ﻫﻤﻜﺎﺭﺍﻥ (١٩٩٨) ﻭﺟﻮﺩ ﺩﺍﺭﺩ .. ﺳﻠﻮﻟﻬﺎﻱ ﺑﺎﻛﺘﺮﻱ ﺗﺮﺟﻴﺤﺎ ﺩﺭ ﻣﺤﻠﻬﺎﻱ ﻏﻨﻲ ﺍﺯ ﻣﻮﺍﺩ ﮔﺰﺍﺭﺵ ﻛﺮﺩﻧﺪ ﻛﻪ ﺩﺭ ﺍﻳﺘﺎﻟﻴﺎ ﺷﺪﺕ ﺑﻴﻤﺎﺭﻱ ﺳﻮﺧﺘﮕﻲ ﺑﺎﻛﺘﺮﻳﺎﻳﻲ ﻏﺬﺍﻳﻲ ﻣﺎﻧﻨﺪ ﻓﺮﻭ ﺭﻓﺘﮕﻲ ﻫﺎﻱ ﺑﺮﮒ، ﺩﺭ ﺍﻣﺘﺪﺍﺩ ﺭﮔﺒﺮﮒ ﺍﺻﻠﻲ ﻭ ﺩﺭ ﺳﻮﻳﺎ ﺩﺭ ﺍﺛﺮ (P. savastanoi pv.glycinea (Psg ﺍﺭﺗﺒﺎﻁ ﻣﻜﺎﺗﺒﻪ ﻛﻨﻨﺪﻩ: ﻣﺼﻄﻔﻲ ﻧﻴﻚ ﻧﮋﺍﺩ ٢١٨ ﻣﺠﻠﻪ ﻋﻠﻮﻡ ﻛﺸﺎﻭﺭﺯﻱ ﺍﻳﺮﺍﻥ، ﺟﻠﺪ ٣٥، ﺷﻤﺎﺭﻩ ١، ﺳﺎﻝ ١٣٨٣ ﻣﺴﺘﻘﻴﻢ ﺑﺎ ﻣﻴﺰﺍﻥ ﺁﻟﻮﺩﮔﻲ ﺑﺬﺭ ﺩﺍﺭﺩ . ﺳﻠﻮﻟﻬﺎﻱ ﺍﻳﻦ ﺑﺎﻛﺘﺮﻱ .P. ، P. tomato .pv. apiii ،P. savastanoi. pv ﺑﺼﻮﺭﺕ ﺍﭘﻲ ﻓﻴﺖ ﺩﺭ ﺑﺬﺭ ﻣﻲ ﺑﺎﺷﻨﺪ . ﺩﺭﺟﻪ ﺣﺮﺍﺭﺕ ﻭ ﺭﻃﻮﺑﺖ، P. tomato. pv. ، savastanoi. pv. passiflorae ﺗﺎﺛﻴﺮ ﺯﻳﺎﺩﻱ ﺑﺮ ﺭﻭﻱ ﺗﻜﺜﻴﺮ ﺑﺎﻛﺘﺮﻳﻬﺎ ﻱ ﺍﭘﻲ ﻓﻴﺖ ﻣﻲ ﮔﺬﺍﺭﺩ . ﺍﻳﻦ ﺍﺛﺮ philadelphi ﻭ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣﺨﺘﻠﻒ .P. tomato pv ﻫﻤﭽﻨﻴﻦ ﺟﻬﺖ ﺍﺳﺘﻘﺮﺍﺭ ﺑﺎﻛﺘﺮﻱ ﻭ ﺗﻮﺳﻌﻪ ﻭ ﺷﺪﺕ ﺑﻴﻤﺎﺭﻱ ﻧﻴﺰ maculicola (١٤) ﺑﺎ ﺳﻮﺳﭙﺎﻧﺴﻴﻮﻥ ﺑﺎﻛﺘﺮﻱ ﺑﺎ ﻏﻠﻈﺖ 2 ﻣﻮﺛﺮ ﺍﺳﺖ (٧) . ﺗﺤﻘﻴﻘﺎﺕ ﻛﻠﻮﻙ ﻭ ﻫﻤﻜﺎﺭﺍﻥ (٢٠٠٠) ﻧﺸﺎﻥ ﺩﺍﺩ cfu / ml ١٠٨×١ ( ﺳﻠﻮﻝ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﻣﻴﻠﻲ ﻟﻴﺘﺮ ) ﻗﺮﺍﺭ ﺩﺍﺩﻩ ﻛﻪ ﺩﺭ Ptt ﺑﺎ ﺍﻟﺤﺎﻕ ﺗﺮﺍﻧﺴﭙﻮﺯﺍﻥ Tn5 ﺩﺭ ﻣﻨﻄﻘﻪ ﮊﻥ dspA ﺷﺪﻧﺪ. ﺳﭙﺲ ﺑﺬﻭﺭ ﺗﻠﻘﻴﺢ ﺷﺪﻩ ﺑﺮ ﺭﻭﻱ ﻛﺎﻏﺬ ﺻﺎﻓﻲ ﺳﺘﺮﻭﻥ ﺑﺎﻋﺚ ﻛﺎﻫﺶ ﻋﻤﻠﻜﺮﺩ ﺳﻴﺴﺘﻢ ﺗﺮﺷﺢ ﭘﺮﻭﺗﺌﻴﻦ ﻧﻮﻉ ﺳﻮﻡ (III) ﺩﺭ ﺧﺸﻚ ﺷﺪﻩ ﻭ ﺑﻼﻓﺎﺻﻠ ﻪ ﺩﺭ ﺩﺍﺧﻞ ﺑﻼﻙ ﻫﺎﻱ ﺳﻮﺭﺍﺧﺪﺍﺭ ﺍﺯ ﺟﻨﺲ ﺳﻠﻮﻟﻬﺎﻱ ﺑﺎﻛﺘﺮ ﻱ ﺷﺪﻩ ﻭ ﺍﻳﻦ ﺍﻣﺮ ﺑﺎﻋﺚ ﻛﺎﻫﺶ ﺗﻜﺜﻴﺮ ﺳﻠﻮﻟﻬﺎﻱ ﭘﻠﻲ ﺍﺳﺘﻴﻠﻦ ( ﺑﻪ ﺍﺑﻌﺎﺩ ٦٠ ×٤٠ ﺳﺎﻧﺘﻲﻣﺘﺮ) ﻣﺤﺘﻮﻱ ﭘﺸﻢ ﺷﻴﺸﻪ ﺑﺎﻛﺘﺮﻱ ﻭ ﺑﻴﻤﺎﺭﻳﺰﺍﻳﻲ ﺩﺭ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﻣﻲ ﺷﻮﺩ . ﻧﻴﻚ ﻧﮋﺍﺩ ﻭ ﻛﺸﺖ ﮔﺮﺩﻳﺪﻧﺪ . ﭘﺸﻢ ﺷﻴﺸﻪ ﻗﺒﻞ ﺍﺯ ﻛﺸﺖ ﺑﺎ ﻣﺤﻠﻮﻝ ﻏﺬﺍﻳﻲ ﻫﻤﻜﺎﺭﺍﻥ ﺍﺛﺮﺍﺕ ﻣﺘﻘﺎﺑﻞ ﮊﻧﻬﺎﻱ Pto/avrPto ﺭﺍ ﺩﺭ ﺣﺎﻟﺖ ﻣﺮﻃﻮﺏ ﺷﺪ. ﻫﺮ ﭘﻼﻙ ﺳﻮﺭﺍﺧﺪﺍﺭ ٣ ٢١ ﻟﻴﺘﺮ ﺍﺯ ﻣﺤﻠﻮﻝ ﻏﺬﺍﻳﻲ ﺭﺍ ﺳﺎﺯﮔﺎﺭﻱ ﻭ ﻧﺎﺳﺎﺯﮔﺎﺭﻱ ﺭﻭﻱ ﻛﻠﻨﻴﺰﺍﺳﻴﻮﻥ ﺑﺎﻛﺘﺮﻱ Ptt ﮔﺰﺍﺭﺵ ﺟﺬﺏ ﻣﻲ ﻧﻤﺎﻳﺪ. ﺑﺬﺭﻫﺎﻱ ﺗﻠﻘﻴﺢ ﺷﺪﻩ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﺑﺮ ﺭﻭﻱ ﻛﺮﺩﻧﺪ ﻭ ﺍﻇﻬﺎﺭ ﻧﻤﻮﺩﻧﺪ ﻛﻪ ﻛﻠﻨﻴﺰﺍﺳﻴﻮﻥ ﺍﭘﻲ ﻓﻴﺖ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﺳﺮﭘﻮﺷﻬﺎﻱ ﭘﺸﻢ ﺷﻴﺸﻪ ﻗﺮﺍﺭ ﺩﺍﺩﻩ ﻭ ﺳﭙﺲ ﺑﻮﺳﻴﻠﻪ ﻭﺭﻣﻴﻜﻮﻟﻴﺖ ﺷﺮﺍﻳﻂ ﻣﺰﺭﻋﻪ ﺩﺭ ﺣﺎﻟﺖ ﺳﺎﺯﮔﺎﺭﻱ ﺑﻪ ﻣﻴﺰﺍﻥ ﺑﺎﻻﺗﺮﻱ ﻧﺴﺒﺖ ﺑﻪ (١٥٠ ﮔﺮﻡ ﻭﺭﻣﻴﻜﻮﻟﻴﺖ ﺑﺮﺍﻱ ﻫﺮ ﭘﻼﻙ ) ﭘﻮﺷﺎﻧﺪﻩ ﺷﺪﻧﺪ . ﭘﻼﻙﻫﺎ ﺣﺎﻟﺖ ﻧﺎﺳﺎﺯﮔﺎﺭﻱ ﺭﻭﻱ ﮔﻴﺎﻩ ﺻﻮﺭﺕ ﻣﻲ ﮔﻴﺮﺩ (٦). ﺩﺭ ﺩﺳﺘﮕﺎﻩ ﺟﻮﺍﻧﻪ ﺯﻧﻲ٤ ﺩﺭ ٢٥ ﺩﺭﺟﻪ ﺳﺎﻧﺘﻲ ﮔﺮﺍﺩ ﺩﺭ ﺭﻭﺯ (١٧ ﻫﺪﻑ ﺍﺯﺍﻳﻦ ﺗﺤﻘﻴﻖ ﺗﻌﻴﻴﻦ ﻣﻴﺰﺍﻥ ﺟﻤﻌﻴﺖ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣﺨﺘﻠﻒ .Pseudomonas spp ﻛﻪ ﺍﺯ ﻧﻈﺮ ﮊﻧﺘﻴﻜﻲ ﻧﺰﺩﻳﻚ ﺑﻪ Ptt ﺑﻮﺩﻩ ﻭ ﺳﺎﻋﺖ) ﻭ ١٨ ﺩﺭﺟﻪ ﺳﺎﻧﺘﻲ ﮔﺮﺍﺩ ﺩﺭ ﺷﺐ (٧ ﺳﺎﻋﺖ ) ﺩﺭ ﺭﻃﻮﺑﺖ ﻫﻤﭽﻨﻴﻦ ﻣﻮﺗﺎﻥ ﻫﺎﻱ hrp ﺁﻧﻬﺎ ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ﻧﺴﺒﻲ ٩٥ ﺩﺭﺻﺪ ﺑﻪ ﻣﺪﺕ ﭼﻬﺎﺭ ﻫﻔﺘﻪ ﻧﮕﻬﺪﺍﺭﻱ ﺷﺪﻧ ﺪ. ﻧﺸﺎ ﻣﺨﺘﻠﻒ ﺭﻭﻱ ﺍ ﻧﺪﺍﻣﻬﺎﻱ ﻣﺨﺘﻠﻒ (ﺑﺮﮒ، ﻣﺮﻳﺴﺘﻢ ﺍﻧﺘﻬﺎﻳﻲ، ﺳﺎﻗﻪ ﻭ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﭘﺲ ﺍﺯ ﺍﻧﺘﻘﺎﻝ ﺑﻪ ﮔﻠﺪﺍﻥ ﺑﻪ ﻣﺪﺕ ٦٠ ﺭﻭﺯ ﺩﺭ ﮔﻠﺨﺎﻧﻪ ﻃﻮﻗﻪ) ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﻣﻲ ﺑﺎﺷﺪ. ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ٦٥ ﺩﺭﺻﺪ ﺩﺭ ﺩﻣﺎﻱ ٢٦ ﺩﺭﺟﻪ ﺳﺎﻧﺘﻲ ﮔﺮﺍﺩ ﻧﮕﻪ ﺩﺍﺭﻱ ﺷﺪﻧﺪ . ﺳﭙﺲ ﺑﺨﺸﻲ ﺍﺯ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﻫﺎ ﺑﻪ ﻣﻮﺍﺩ ﻭ ﺭﻭﺵﻫﺎ ﺍﻃﺎﻗﻚ ﺭﺷﺪ ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ٩٥ ﺩﺭﺻﺪ ﺩﺭ ﺩﻣﺎﻱ ٢٦ ﺩﺭ ﺍﻳﻦ ﺑﺮﺭﺳﻲ ﻭﺍﺭﻳﺘﻪ ﻛﺎﻧﺮﻳﺮﻭ ١ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﻛﻪ ﺣﺴﺎﺱ ﺑﻪ ﺩﺭﺟﻪ ﺳﺎﻧﺘﻲ ﮔﺮﺍﺩ (١٧ ﺳﺎﻋﺖ ) ﺩﺭ ﺭﻭﺯ ﻭ ٢٠ ﺩﺭﺟﻪ ﺳﺎﻧﺘﻲ ﮔﺮﺍﺩ ﺩﺭ ﺑﺎﻛﺘﺮﻱ Ptt ﺍﺳﺖ ﺍﺳﺘﻔﺎﺩﻩ ﺷﺪ. ﻗﺒﻞ ﺍﺯ ﻛﺸﺖ، ﺟﻬﺖ ﺿﺪﻋﻔﻮﻧﻲ ﺷﺐ ﻣﻨﺘﻘﻞ ﺷﺪﻧﺪ. ٢- ﺟﺪﺍﺳﺎﺯﻱ ﻭ ﺷﻤﺎﺭﺵ ﺑﺎﻛﺘﺮﻳﻬﺎ ﺩﺭ ﮔﻴﺎﻩ ﺳﻄﺤﻲ، ﺑﺬﺭﻫﺎﻱ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﺭﺍ ﺑﻪ ﻣﺪﺕ ٥ ﺩﻗﻴﻘﻪ ﺩﺭ ﻣﺤﻠﻮﻝ ﭘﺲ ﺍﺯ ٢١ ﺭﻭﺯ ﺑﺮﺍﻱ ﻫﺮ ﮔﻴﺎﻫﭽﻪ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﺑﻄﻮﺭ ﺟﺪﺍﮔﺎﻧﻪ ﻫﻴﭙﻮﻛﻠﺮﻳﺖ ﺳﺪﻳﻢ (٦/٠ ﺩﺭﺟﻪ ﻛﻠﺮ ) ﻗﺮﺍﺭ ﺩﺍﺩﻩ ﺷﺪ ﻭ ﺳﭙﺲ ﺳﻪ ٥ ﺑﺎﺭ ( ﻫﺮ ﺑﺎﺭ ١٠ ﺩﻗﻴﻘﻪ ) ﺑﺎ ﺁﺏ ﻣﻘﻄﺮ ﺳﺘﺮﻭﻥ ﺷﺴﺘﺸﻮ ﺩﺍﺩﻩ ﺷﺪﻧﺪ . ﺩﺭ ﺩﺍﺧﻞ ﻛﻴﺴﻪ ﭘﻼﺳﺘﻴﻜﻲ ﺍﺳﺘﻮﻣﺎﺷﺮ ﺳﺘﺮﻭﻥ ﻭ ﺑﺮﺍﻱ ﻫﺮ ﻗﺴﻤﺖ ﺟﻬﺖ ﺧﺸﻚ ﻛﺮﺩﻥ ﺑﺬﺭﻫﺎ ، ﺁﻧﻬﺎ ﺭﺍ ﺭﻭﻱ ﻛﺎﻏﺬﻫﺎﻱ ﺻﺎﻓﻲ ﺳﺘﺮﻭﻥ ﺍﺯ ﮔﻴﺎﻩ ﻛﺎﻣﻞ (ﻣﺮﻳﺴﺘﻢ ﺍﻧﺘﻬﺎﻳﻲ، ﺑﺮﮔﻬﺎﻱ ﻭ ﺳﺎﻗﻪ ﺍﻧﺘﻬﺎﻳﻲ، ﺑﺮﮔﻬﺎﻱ ﺩﺭ ﺍﻃﺎﻗﻚ ﻛﺸﺖ ﺩﺭ ﺯﻳﺮ ﻧﻮﺭ ﻓﻠﻮﺭﺳﻨﺖ ﺑﻪ ﻣﺪﺕ ٣٠ ﺩﻗﻴﻘﻪ ﻗﺮﺍﺭ ﻭ ﺳﺎﻗﻪ ﻣﻴﺎﻧﻲ، ﺑﺮﮔﻬ ﺎ ﻭ ﺳﺎﻗﻪ ﭘﺎﻳﻴﻨﻲ، ﻃﻮﻗﻪ، ﺭﻳﺸﻪ ﻫﺎﻱ ﺍﺻﻠﻲ ﻭ ﺩﺍﺩﻩ ﺷﺪﻧﺪ . ﺭﻳﺸﻪ ﻫﺎﻱ ﻓﺮﻋﻲ ) ١ ﺗﺎ ٥ ﻣﻴﻠﻲ ﻟﻴﺘﺮ ﺁﺏ ﻣﻘﻄﺮ ﺳﺘﺮﻭﻥ ﺍﺿﺎﻓﻪ ١- ﺗﻠﻘﻴﺢ ﺑﺬﺭﻫﺎﻱ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﺑﺎ ﺑﺎﻛﺘﺮﻱ ﻭ ﻛﺸﺖ ﺁﻧﻬﺎ ﮔﺮﺩﻳﺪ. ﺳﭙﺲ ﺑﻮﺳﻴﻠﻪ ﻳﻚ ﻏﻠﻄﻚ ﭘﻼﺳﺘﻴﻜﻲ، ﮔﻴﺎﻫﭽﻪ ﺑﺬﺭﻫﺎﻱ ﮔﻮﺟﻪ ﻓﺮﻧﮕﻲ ﺑﻌﺪ ﺍﺯ ﺿﺪﻋﻔﻮﻧﻲ ﺳﻄﺤﻲ ﻭ ﺧﺸﻚ ﮔﻮﺟﻪﻓﺮﻧﮕﻲ ﺩﺭ ﺩﺍﺧﻞ ﻛﻴﺴﻪ ﭘﻼﺳﺘﻴﻜﻲ ﻛﺎﻣﻼ ﻟﻪ ﮔﺮﺩﻳﺪ ﻭ ﻋﺼﺎﺭﻩ ٦- ﺷﺪﻥ، ﺑﻪ ﻣﺪﺕ ٢ ﺳﺎﻋﺖ ﺩﺭ ﺗﻮﺳﻂ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣﺨﺘﻠﻒ ﮔﻴﺎﻩ ﺑﻮﺳﻴﻠﻪ ﺁﺏ ﻣﻘﻄﺮ ﺳﺘﺮﻭﻥ ﺑﻪ ﻣﻴﺰﺍﻥ ١٠ ×١ ﺭﻗﻴﻖ ﺷﺪﻧﺪ .Pseudomonas spp ﻛﻪ ﺍﺯ ﻧﻈﺮ ﮊﻧﺘﻴﻜﻲ ﻧﺰﺩﻳﻚ ﺑﻪ Ptt ﺑﻮﺩﻧﺪ ﻛﻪ ﺷﺎﻣﻞ colony forming unit lachrymans ،P. tomato. pv. berberidis .2 3. plaque alveolee 4. germinator 5. stomachair 1. cannery row ﻧﻴﻚ ﻧﮋﺍﺩ ﻛﺎﻇﻢ ﭘﻮﺭ ﻭ ﻣﺎﻧﺴﻮ: ﻣﻄﺎﻟﻌﻪ ﺩﻳﻨﺎﻣﻴﺰﻡ ﺟﻤﻌﻴﺖ ﺑﺮﺧﻲ ﺟﺪﺍﻳﻪ ﻫﺎﻱ... ٢١٩ ﻭ ﺳ ﭙﺲ ﺍﺯ ﻫﺮ ﻏﻠﻈﺖ ﺑﻪ ﻣﻴﺰﺍﻥ ٥٠ (ﻣﻴﻜﺮﻭﻟﻴﺘﺮ) ﺑﻮﺳﻴﻠﻪ ﺳﺎﻳﺮ ﮔﻴﺎﻫﺎﻥ ﻣﺎﻳﻪ ﺯﻧﻲ ﺷﺪﻩ ﻣﺎ ﺑﻴﻦ ﺭﺷﺪ ﻃﻮﻟﻲ ﮔ ﻴﺎﻫﺎﻥ ﺷﺎﻫﺪ ﻭ ﻣﻴﻜﺮﻭﭘﻴﭙﺖ ﺑﺮﺩﺍﺷﺘﻪ ﻭ ﺑﺮ ﺭﻭﻱ ﻣﺤﻴﻂ (١٥) ,.King et al) ﮔﻴﺎﻫﺎﻥ ﻣﺎﻳﻪ ﺯﻧﻲ ﺷﺪﻩ ﺑﺎ ﺟﺪﺍﻳﻪ ٨٢٠٧ ﺑﻮﺩ . ﺗﻤﺎﻣﻲ ﺟﺪﺍﻳﻪ ﻫﺎﻱ King B ( 1954 ﻣﺤﺘﻮﻱ ٥٠ (ﻣﻴﻜﺮﻭ ﻟﻴﺘﺮ ﺩﺭ ﻣﻴﻠﻲ ﻟﻴﺘﺮ) ﺁﻧﺘﻲ ﻣﺨﺘﻠﻒ Pseudomonas ﺑﺮ ﺭﻭﻱ ﮔﻴﺎﻫﺎﻧﻲ ﻛﻪ ﺩﺭ ﺍﻃﺎﻗﻚ ﺭﺷﺪ ﺑﻴﻮﺗﻴﻚ ﺍﻛﺘﻴﺪﻳﻮﻥ ، ﻛﺸﺖ ﮔﺮﺩﻳﺪ . ﺑﻄﻮﺭﻳﻜﻪ ﺩﺭ ﻫﺮ ﺗﺸﺘﻚ ، (ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ٩٥ ﺩﺭﺻﺪ ) ﻧﮕﻪ ﺩﺍﺭﻱ ﺷﺪﻩ ﺑﻮﺩﻧﺪ ﺑﺎﻋﺚ ﺍﻳﺠﺎﺩ ﻋﺼﺎﺭﻩ ﮔﻴﺎﻩ ﺁﻟﻮﺩﻩ ﺗﺎ ﻏﻠﻈﺖ ٦-١٠ ×١ ﻗﺮﺍﺭ ﺩﺍﺩﻩ ﺷﺪ . ﺑﺮﺍﻱ ﻫﺮ ﻟﻜﻪ ﻫﺎﻱ ﻧﻜﺮﻭﺗﻴﻚ ﮔﺮﺩﻳﺪﻧﺪ . ﺗﻨﻬﺎ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ٨٢٠٧ Ptt ﻭ ﺗﻴﻤﺎﺭ ٥ ﮔﻴﺎﻩ (ﺗﻜﺮﺍﺭ) ﻭ ﺑﺮﺍﻱ ﻫﺮ ﮔﻴﺎﻩ ٣ ﺗﺸﺘﻚ ﭘﺘﺮﻱ ﺩﺭ ﻧﻈﺮ P.t.pv.maculicola١٧٤٠ ﺑﺎﻋﺚ ﺍﻳﺠﺎﺩ ﻧﻜﺮﻭﺯ ﻛﺎﻣﻞ ﮔﻴﺎﻩ ﺷﺪﻧﺪ. ﮔﺮﻓﺘﻪ ﺷﺪ . ﺳﭙﺲ ﺗﺸﺘﻚ ﻫﺎﻱ ﭘﺘﺮﻱ ﺭﺍ ﺩﺭ ﺩﻣﺎﻱ ٢٧ ﺩﺭﺟﻪ ﻧﺘﺎﻳﺞ ﺑﺪﺳﺖ ﺁﻣﺪﻩ ﺗﻮﺳﻂ ﻣﺎﻟﺶ ﭘﻨﺒﻪ ﺁﻏﺸﺘﻪ ﺑﺎ ﺳﻮﺳﭙﺎﻧﺴﻴﻮﻥ ﺳﺎﻧﺘﻴﮕﺮﺍﺩ ﻗﺮﺍﺭ ﺩﺍﺩﻩ ﺷﺪﻧﺪ ﻭ ﺑﻌﺪ ﺍﺯ ٧٢ ﺳﺎﻋﺖ ، ﻛﻠﻨﻲ ﻫﺎﻱ ﺑﺎﻛﺘﺮﻱ (ﻏﻠﻈﺖ ١٠٨ ×١) ﺩﺭ ﻫﻤﻪ ﭘﺎﺗﻮﺍﺭﻫﺎﻱ ﻣﻮﺭﺩ ﻣﻄﺎﻟﻌﻪ ﺭﻭﻱ ﻓﻠﻮﺭﺳﻨﺖ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﺯﻳﺮ ﻧﻮﺭ ﻣﺎﻭﺭﺍﺀ ﺑﻨﻔﺶ ﺷﻤﺎﺭﺵ ﮔﺮﺩﻳﺪﻧﺪ . ﮔﻴﺎﻫﺎﻧﻴﻜﻪ ﺩﺭ ﺷﺮﺍﻳﻂ ﮔﻠﺨﺎﻧﻪ (ﺭﻃﻮﺑﺖ ﻧﺴﺒﻲ ٦٥ ﺩﺭﺻﺪ ) ﻧﮕﻪ ﺩﺍﺭﻱ ﺟﻬﺖ ﺑﺮﺁﻭﺭﺩ ﻣﻴﺰﺍﻥ ﺟﻤﻌﻴﺖ ﺑﺎﻛﺘﺮﻱ ﺩ ﺭ ﮔﻴﺎﻩ ﺍﺯ ﻓﺮﻣﻮﻝ ﺯﻳﺮ ﺷﺪﻩ ﺑﻮﺩﻧﺪ ﺑﺎﻋﺚ ﺍﻳﺠﺎﺩ ﻟﻜﻪ ﻫﺎﻱ ﻧﻜﺮﻭﺗﻴﻚ ﮔﺮﺩﻳﺪ . ﺑﺠﺰ ﺟﺪﺍﻳﻪ ﺍﺳﺘﻔﺎﺩﻩ ﺷﺪ . ١٦٢٠ P.t.pv.anttirrhini ﻋﻼﺋﻢ ﻧﻜﺮﻭﺗﻴﻚ ﺗﻨﻬﺎ ﺩﺭ ﮔﻴﺎﻫﺎﻧﻲ N = X . V . D. 20 ﻛﻪ ﺗﺤﺖ ﺷﺮﺍﻳﻂ ﺍﻃﺎﻗﻚ ﺭﺷﺪ ﻧﮕﻪ ﺩﺍﺭﻱ ﻣﻲ ﺷﺪﻧﺪ ﺑﻮﺟﻮﺩ ﺁﻣﺪ . N = ﺗﻌﺪﺍﺩ ﺳﻠﻮﻝ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﻫﺮ ﮔﻴﺎﻩ ﺩﺭ ﺷﺮﺍﻳﻂ ﮔﻠﺨﺎﻧﻪ ﮔﻴﺎﻫﺎﻥ ﻣﺎﻳﻪ ﺯﻧﻲ ﺷﺪﻩ ﺑﺎ ﺍﻳﻦ ﺟﺪﺍﻳﻪ ﻫﻴﭽﮕﻮﻧﻪ X = ﺗﻌﺪﺍﺩ ﻛﻠﻨﻲ ﻫﺎﻱ ﺑﺎﻛﺘﺮﻱ ﺷﻤﺎﺭﺵ ﺷﺪﻩ ﺩﺭ ﻫﺮ ﻏﻠﻈﺖ ﻋﻼﺋﻤﻲ ﺍﺯ ﺧﻮﺩ ﺑﺮﻭﺯ ﻧﺪﺍﺩﻧﺪ (ﺟﺪﻭﻝ ١). ﻣﻴﺰﺍﻥ ﺟﻤﻌﻴﺖ ﺑﺎﻛﺘﺮﻱ D = ﻓﺎﻛﺘﻮﺭ ﻏﻠﻈﺖ ﺭﻭﻱ ﮔﻴﺎﻫﺎﻥ ﻣﺎﻳﻪ ﺯﻧﻲ ﺷﺪﻩ ﻛﻪ ﺩﺭ ﺷﺮﺍﻳﻂ ﺍﻃﺎﻗﻚ ﺭﺷﺪ ﻧﮕﻪ ﺩﺍﺭﻱ V = ﺣﺠﻢ ﺁﺏ ﻣﻘﻄﺮﻱ ﻛﻪ ﺑﻪ ﮔﻴﺎﻩ ﺩﺭ ﻛﻴﺴﻪ ﭘﻼﺳﺘﻴﻜﻲ ﺷﺪﻩ ﺑﻮﺩﻧﺪ ﺩﺭ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣﺨﺘﻠﻒ .Pseudomonas spp ﺩﺭ ﺍﺿﺎﻓﻪ ﺷﺪ ﺟﺪﻭﻝ ١ ﻧﺸﺎﻥ ﺩﺍﺩﻩ ﺷﺪ . ﻧﺘﺎﻳﺞ ﺁﻧﺎﻟﻴﺰ ﺁﻣﺎﺭﻱ ﻧﺸﺎﻥ ﺩﺍﺩ ﻛﻪ ﻣﻴﺰﺍﻥ ٣- ﺁﻧﺎﻟﻴـﺰ ﺁﻣﺎﺭﻱ ﺟﻤﻌﻴﺖ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﮔﻮﻧﻪ ﻫﺎﻱ ﻣﺨﺘﻠﻒ ﺑﺎ ﻳﻜﺪﻳﮕﺮ ﺗﻔﺎﻭﺕ ﭘﺲ ﺍﺯ ﺷﻤﺎﺭﺵ ﻛﻠﻨﻲ ﻫﺎﻱ ﺑﺎﻛﺘﺮﻱ ﺍﺯ ﺭﻭﻱ ﺗﺸﺘﻚ ﭘﺘﺮﻱ، ﻣﻌﻨﻲ ﺩﺍﺭﻱ ﻭﺟﻮﺩ ﺩﺍﺭﻧﺪ. ﻣﻴﺰﺍﻥ ﺗﻌﺪﺍﺩ ﺳﻠﻮﻟﻬﺎﻱ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﻫﺮ ﮔﻴﺎﻩ ﺑﻪ ﻟﮕﺎﺭﻳﺘﻢ ﺍﻋﺸﺎﺭﻱ ٢ - ﻧﺤﻮﻩ ﭘﺨﺶ ﺳﻠﻮﻟﻬﺎﻱ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﺍﻧﺪﺍﻣﻬﺎﻱ ﻣﺨﺘﻠﻒ ﮔﻴﺎﻩ ﺗﺒﺪﻳﻞ ﮔﺮﺩﻳﺪ . ﻳﻜﻨﻮﺍﺧﺘﻲ ﻭﺍﺭﻳﺎﻧﺲ ﺑﻮﺳﻴﻠﻪ ﺁﺯﻣﻮﻥ ﺑﺎﺭﺗﻠﺖ (١٩٩٣) ﭘﺲ ﺍﺯ ٢١ ﺭﻭ ﺯ ﻧﮕﻪ ﺩﺍﺭﻱ ﮔﻴﺎﻫﺎﻥ ﺩﺭ ﮔﻠﺨﺎﻧﻪ ﻭ ﺍﻃﺎﻗﻚ ﺭﺷﺪ، ﻛﻨﺘﺮﻝ ﮔﺮﺩﻳﺪ . ﺩﺭ ﺻﻮﺭﺗﻴﻜﻪ ﻭﺍﺭﻳﺎﻧﺲ ﻫﺎ ﺗﻮﺳﻂ ﺁﺯﻣﻮﻥ ﺑﺎﺭﺗﻠﺖ ﻣﻴﺰﺍﻥ ﺟﻤﻌﻴﺖ ﺟﺪﺍﻳﻪ ﻫﺎﻱ ﻣﺨﺘﻠﻒ ﺑﺎﻛﺘﺮﻱ ﺩﺭ ﺍﻧﺪﺍﻣﻬﺎﻱ ﻣﺨﺘﻠﻒ ﻳﻜﻨﻮﺍﺧﺖ ﺗﺸﺨﻴﺺ ﺩﺍﺩﻩ ﺷﺪﻧﺪ، ﺁﻧﺎﻟﻴﺰ ﻭﺍﺭﻳﺎﻧﺲ ﺑﻮﺳﻴﻠﻪ ﺁﺯﻣﻮﻥ ﮔﻴﺎﻩ ﺗﻌﻴﻴﻦ ﮔﺮﺩﻳﺪ. ﻓﻴﺸﺮ ﺩﻧﺒﺎﻝ ﮔﺮﺩﻳﺪ . ﺍﮔﺮ ﺍﺭﺯﺵ ﺩﺍﺩﻩ ﻫﺎﻱ ﻣﺤﺎﺳﺒﻪ ﺷﺪﻩ ﻛﻮﭼﻜﺘﺮ ﺍﺯ ١-٢ – ﮔﻴﺎﻫﺎﻥ ﻣﺎﻳﻪ ﺯﻧﻲ ﺷﺪﻩ ﺩﺭ ﺷﺮﺍﻳﻂ ﮔﻠﺨﺎﻧﻪ F ﺩﺭﺟﺪﻭﻝ ﻓﻴﺸﺮ ﺑﻮﺩ ﺩﺭ ﺍﻳﻦ ﺻﻮﺭﺕ ﺗﻔﺎﻭﺕ ﻣﻌﻨﻲ ﺩﺍﺭﻱ ﺑﻴﻦ ﺟﺪﺍﻳﻪ ﺑﻴﻤﺎﺭﻳﺰﺍ ٨٢٠٧ Ptt ﺗﻤﺎﻣﻲ ﻗﺴﻤﺘﻬﺎﻱ ﮔﻴﺎﻩ ﺭﺍ ﻛﻠﻨﻴﺰﻩ ﺩ ﺍﺩﻩﻫﺎﻱ ﻣﻮﺭﺩ ﻣﻄﺎﻟﻌﻪ ﻭﺟﻮﺩ ﻧﺪﺍﺭﺩ .
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