Roland Hatzenpichler Curriculum vitae
Roland S. Hatzenpichler, PhD
California Institute of Technology Division of Geological and Planetary Sciences 1200 E. California Blvd, MC 100-23, Pasadena, CA-91125 phone: 626-319-2707 [email protected] www.gps.caltech.edu/content/roland-s-hatzenpichler
Curriculum vitae
Postdoc, current position since July 2012, Marie Curie Fellow, Postdoctoral Scholar in Geobiology Division of Geological and Planetary Sciences (GPS), California Institute of Technology Advisor: Victoria Orphan, Professor of Geobiology
July 2011 - June 2012, O.K. Earl Postdoctoral Scholar in Geobiology, GPS Division, Caltech Advisor: Victoria Orphan, Professor of Geobiology
Doctor of natural sciences (PhD) May 2006 - June 2011, Department of Microbial Ecology, University of Vienna, Austria Advisor: Michael Wagner, Full Professor for Microbial Ecology Thesis: Identification and characterization of novel ammonia-oxidizing archaea and bacteria Title of qualification: Doctor rerum naturalium (Doctor of natural sciences, PhD)
Graduation with perfect grade point average Three year PhD student fellowship (DOC) of the Austrian Academy of Sciences, 2007-2009 Doc.Award for outstanding PhD thesis by the City of Vienna
Master of science April 2005 - April 2006, Department of Microbial Ecology, University of Vienna, Austria Advisor: Michael Wagner, Full Professor for Microbial Ecology Thesis: Diversity analyses and in situ detection of nitrifying prokaryotes in hot springs and primeval forest soil Title of qualification: Magister rerum naturalium (Master of natural sciences) Passed with distinction
Undergraduate studies October 2001 - April 2005, University of Vienna, Austria Study of microbiology and genetics, with focus on environmental microbiology, molecular microbiology and genetics, as well as analytical and organic chemistry Completion of studies with distinction
Additional training June 13 - July 30, 2009, Microbial Diversity course, Marine Biological Laboratory, Woods Hole (MA), USA; Advisors: Associate Prof. Daniel Buckley and Prof. Stephen H. Zinder
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Roland Hatzenpichler Curriculum vitae
Scientific experience
Publications, >670 citations (source: google scholar) equal contribution, *corresponding Lebedeva EV, Hatzenpichler R, Pelletier E, Schuster N, Hauzmayer S, Bulaev A, Grigorjeva NV, Galushko A, Schmid M, Palatinsky M, Le Paslier D, Daims H, and Wagner M. Enrichment and genome sequence of the group I.1a ammonia-oxidizing archaeon “Ca. Nitrosotenuis uzonensis” representing a clade globally distributed in thermal habitats. PLoSOne, 8: e80835 (2013) Hatzenpichler R*. Diversity, physiology and niche differentiation of ammonia-oxidizing archaea. Appl Environ Microbiol, 78: 7501-7510 (2012) [Review article] Spang A, Poehlein A, Offre P, Zumbrägel S, Haider S, Rychlik N, Nowka B, Schmeisser C, Lebedeva E, Rattei T, Bӧhm C, Schmid M, Galushko A, Hatzenpichler R, Weinmaier T, Daniel R, Schleper C, Spieck E, Streit W, and Wagner M. The genome of the ammonia- oxidizing Candidatus Nitrososphaera gargensis: Insights into metabolic versatility and environmental adaptations. Environ Microbiol, 14: 3122–3145 (2012) Mußmann M, Brito I, Pitcher A, Damsté JS, Hatzenpichler R, Richter A, Nielsen JL, Nielsen P H, Müller A, Daims H, Wagner M, and Head IM. Thaumarchaeotes abundant in refinery nitrifying sludges express amoA but are not obligate autotrophic ammonia oxidizers. Proc Natl Acad Sci U.S.A., 108: 16771-16776 (2011) Shapiro OH, Hatzenpichler R*, Buckley DH, Zinder SH, and Orphan VJ. Multicellular photo-magnetotactic bacteria. Environ Microbiol Rep, 3: 233–238 (2011) [Chief Editor’s Choice Article 2011] Spang A, Hatzenpichler R, Brochier-Armanet C, Rattei T, Tischler P, Spieck E, Streit W, Stahl DA, Wagner M, and Schleper C. Distinct gene set in two different lineages of ammonia- oxidizing archaea supports the phylum Thaumarchaeota. Trends Microbiol 18:331-40 (2010) Hatzenpichler R, Lebedeva EV, Spieck E, Stoecker K, Richter A, Daims H, and Wagner M. A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring. Proc Natl Acad Sci U.S.A., 105: 2134-2139 (2008)
Manuscripts in review or in preparation *corresponding Hatzenpichler R*, Scheller S, Tavormina PL, Babin B, Tirrell D, and Orphan VJ*. In situ visualization of newly synthesized proteins in environmental microbes via click chemistry. Environ Microbiol, In revision Tavormina PL, Hatzenpichler R, McGlynn S, Chadwick G, Dawson K, Connon S, and Orphan VJ. Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the Deep Sea 1 clade of marine methanotrophs. Int J Syst Evo Microbiol, In review Ma L, Hatzenpichler R, Kim J, Chang G, and Ismagilov RF. Targeted cultivation and isolation of a previously uncultured microbe from the human gut implemented with microfluidics and guided by “chip-wash” and PCR. In prep for Proc Natl Acad Sci U.S.A. Haider S, Stoecker K, Hoshino T, Hatzenpichler R, Bendinger B, and Wagner M. Metagenomics and in situ RCA-FISH reveal that some Crenothrix polyspora strains possess a conventional particulate methane monooxygenase. In prep for Environ Microbiol
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Roland Hatzenpichler Curriculum vitae
Grants and fellowships totaling $~339,000 Marie Curie Fellow via an Erwin Schrӧdinger Postdoctoral Scholarship of the Austrian Science Fund (FWF) at Caltech (2011) [$90,000] O.K. Earl Postdoctoral Fellowship in Geobiology by the Division of Geological and Planetary Sciences of Caltech (2011) [$125,000] Three year PhD student fellowship of the Austrian Academy of Sciences (2007) [~$124,000]
Awards, scholarships & competitive travel grants totaling $~13,500 Doc.Award for outstanding PhD thesis by the City of Vienna. Each year, this prize is awarded to 5-10 PhD-theses completed at the University of Vienna (~91,000 students). In 2012, only three researchers received the award in a field of natural sciences. Two travel awards by the International Society for Microbial Ecology, ISME (2008, 2012) Two scholarship awards by the Gordon and Betty Moore foundation (2009, 2010) Scholarship award by the University of Vienna, Austria (2009) Scholarship award by the Austrian federal state Styria (2009) Travel award of the University of Vienna, Austria (2008) Poster award of the Thermophiles conference. Bergen, Norway (2007) Three travel awards by the European Astrobiology Network Association, EANA (2005-2007)
Invited talks and seminars Plenary talk at the American Society for Microbiology General Meeting (May 2014) Department of Biology, California State University, Northridge, CA (2014) Department of Environmental Systems Science, Swiss Federal Institute of Technology (ETH) Zurich, Switzerland (2013) Jet Propulsion Laboratory, NASA, Pasadena, CA (2013) Department of Microbiology, Montana State University, Bozeman, MT (2011) Division of Geological and Planetary Sciences, Caltech, Pasadena, CA (2010) Institute of Biophysical Chemistry, University of Vienna, Austria (2010) Centre for Marine Bio-Innovation, University of New South Wales, Sydney, Australia (2008)
Contributed communications Seven contributed talks at international conferences or workshops (since 2007) 13 first author posters presentations at international conferences or workshops (since 2007) Three co-authored talks & 13 co-authored posters (since 2009)
Synergistic activities Organizer of the 7th International workshop on New Techniques In Microbial Ecology (2009). [50 participants from six institutions in Austria, Denmark, Germany, Great Britain and US] Regular ad hoc reviewer for the journals Antonie van Leeuwenhoek Journal of Microbiology, Applied and Environmental Microbiology, Environmental Microbiology, Environmental Microbiology Reports, Environmental Science and Technology, FEMS Microbiology Ecology, Microbiology, PLoS One, Nature Scientific Reports, and The ISME Journal External reviewer for grant proposal to NASA’s Exobiology & Evolutionary Biology Program Reviewer for a book chapter on techniques to study single cell ecophysiology
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Roland Hatzenpichler Curriculum vitae
Teaching experience Advisor and mentor of students and guest scientists: Lewis Ward, graduate student at Caltech (since 2013) Julia Brown, undergraduate student at Caltech (since 2013) Grayson Chadwick, undergraduate student at Caltech (2011-2013) Kristen Brileya, visiting graduate student from Montana State University, Bozeman (2010) Nathalie Schuster, master student at DOME, University of Vienna, Austria (2008-2009) Gilbert Flores, visiting graduate student from Portland State University, OR (2008) Michael Cunliffe, visiting postdoc from the University of Warwick, Coventry, UK (2006) Regular supervisor, organizer and lecturer in the annual International FISH course (aimed at the graduate and postgraduate level). DOME, University of Vienna, Austria (2007-2011) Regular supervisor of the annual 2-week undergraduate student course FISH: Identification of uncultivated microorganisms. DOME, University of Vienna, Austria (2007-2010)
Laboratory experience methods of particular importance in my future research Postdoc: development of an approach to visualize, enrich, and identify newly synthesized proteins in uncultured microbes; immunological detection of enzymes in anaerobically methane-oxidizing microbial consortia using a combination of fluorescence microscopy and dynamic nano-scale secondary ion mass spectrometry (nanoSIMS); metaproteomics of microbial consortia from deep-sea sediment; enrichment and cultivation of anaerobic microbes, most importantly sulphate reducers, methanogens, and methanotrophs PhD thesis: enrichment and cultivation of aerobic ammonia-oxidizing archaea and bacteria; physiological characterization of microbes on the single cell level using different fluorescent and halogen in situ hybridization techniques (FISH) coupled to the detection of radioactive and stable isotope labelling via micro-autoradiography (MAR), nanoSIMS and Raman micro- spectroscopy; immunofluorescence; (meta)genomics; in-depth phylogenetic analyses; laser micro-dissection; confocal laser scanning and epifluorescence microscopy Microbial diversity course: isolation, cultivation, and molecular characterization of a diverse range of microbes, including phototrophs, bioluminescent, and magnetotactic bacteria Master thesis: environmental sampling; DNA/RNA/protein isolation; PCR; denaturing gradient gel electrophoresis (DGGE); in silico sequence analysis & phylogeny; different FISH techniques, including CARD-FISH, clone-FISH and mRNA-FISH; microarray analyses
Public outreach Chair and judge of the microbiology section of the 62nd, 63rd & 64th annual Los Angeles County Science & Engineering Fair. Pasadena, CA (2012-2014) [science fairs] Carl Woese, (R)evolutionary Biologist. Web article for Wired magazine (2013) You are never alone! Public talk on microbial ecology, hosted by the Technical Museum of Vienna, Austria (2009). This talk was held as finalist of Fame Lab Austria, a national contest on the public communication of science. [Fame Lab national finalist] Microorganisms - the unseen majority. Public talk on environmental microbiology, hosted by the Austrian Academy of Sciences. Vienna, Austria (2008) [talk] Genetic engineering. Yesterday, today, tomorrow. Two public talks, one of them hosted by the municipality of (my hometown) Trofaiach, Austria (2004 & 2005). [two talks]
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Roland Hatzenpichler Curriculum vitae
Professional memberships American Society for Microbiology (ASM); International Society for Microbial Ecology (ISME); Austrian Scientists and Scholars in North America (ASCINA)
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