Roland Hatzenpichler Curriculum vitae

Roland S. Hatzenpichler, PhD

Postdoctoral Scholar in California Institute of Technology Division of Geological and Planetary Sciences 1200 E. California Blvd, MC 100-23, Pasadena, CA-91125 phone: 626-319-2707 email: [email protected]

www.environmental-microbiology.com

Curriculum vitae

Postdoc (current position) Division of Geological and Planetary Sciences, California Institute of Technology Advisor: Victoria Orphan, Professor of Geobiology

since July 2014 Postdoctoral Scholar in Geobiology, supported via a Fellowship by the Center for Dark Energy Biosphere Investigations (C-DEBI) July 2012 - July 2014 Marie Curie Fellow via an Erwin Schrődinger Postdoctoral Scholarship July 2011 - June 2012 Divisional O.K. Earl Postdoctoral Scholar in Geobiology

Doctor of natural sciences (PhD), perfect grade point average May 2006 - June 2011, Department of Microbial Ecology, University of Vienna, Austria Advisor: Michael Wagner, Full Professor for Microbial Ecology Thesis: Identification and characterization of novel ammonia-oxidizing and Title of qualification: Doctor rerum naturalium (Doctor of natural sciences, PhD)

Master of natural science, with distinction April 2005 - April 2006, Department of Microbial Ecology, University of Vienna, Austria Advisor: Michael Wagner, Full Professor for Microbial Ecology Thesis: Diversity analyses and in situ detection of nitrifying prokaryotes in hot springs and primeval forest soil Title of qualification: Magister rerum naturalium (Master of natural sciences)

Undergraduate studies, with distinction October 2001 - April 2005, University of Vienna, Austria Study of microbiology and genetics; major in molecular microbiology and molecular genetics; minors in microbial ecology, analytical chemistry, and organic chemistry

Additional training June 13 - July 30, 2009, Microbial Diversity course, Marine Biological Laboratory, Woods Hole (MA), USA Advisors: Associate Professor Daniel Buckley and Professor Stephen H. Zinder Research project: Multicellular photo-magnetotactic bacteria

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Roland Hatzenpichler Curriculum vitae

Scientific experience

Grants and honors totaling $607,700 • 2014, Director Discretionary project of the Joint Genome Institute, US Department of Energy. Role: Co-PI together with Victoria J. Orphan [$137,000 research grant] • 2014, Postdoctoral Fellowship by the Center for Dark Energy Biosphere Investigations for work at Caltech [$125,000 salary and travel support over 2 years] • 2011, Marie Curie Fellow via an Erwin Schrӧdinger Postdoctoral Scholarship of the Austrian Science Fund at Caltech [$95,000 salary and travel support over 2 years] • 2011, O.K. Earl Postdoctoral Fellowship in Geobiology by the Division of Geological and Planetary Sciences of Caltech [$125,000 salary and travel support over 2 years] • 2011, Doc.Award for outstanding PhD thesis by the City of Vienna. Each year, this prize is awarded to 5-10 PhD-theses completed at the University of Vienna (~91,000 students). In that year, three researchers received the award in a field of natural sciences. [$1,700] • 2007, Three year Pre-doctoral fellowship of the Austrian Academy of Sciences [$124,000 salary support over 3 years]

Invited talks and seminars • 2015, Gordon Research Conference on Applied & Environmental Microbiology, South Hadley, MA • 2015, Department of Environmental Sciences, University of Basel, Switzerland • 2015, Civil and Environmental Engineering, Northeastern University, Boston, MA • 2015, Chair of Microbiology and Archaea-Center, University of Regensburg, Germany • 2015, Faculty of Geosciences, Ludwig Maximilian University, Munich, Germany • 2015, Micro-Morning seminar, Center for Environmental Microbial Interactions, Caltech, Pasadena, CA • 2014, Japan Agency for Marine-Earth Science and Technology, Yokosuka, Japan • 2014, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan • 2014, Planetary Sciences seminar, Division of Geological & Planetary Sciences, Caltech, Pasadena, CA • 2014, Joint Genome Institute, US Department of Energy, Walnut Creek, CA • 2014, Plenary talk, American Society for Microbiology General Meeting, Boston, MA • 2014, Department of Biology, California State University, Northridge, CA • 2013, Department of Environmental Systems Science, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland • 2013, Jet Propulsion Laboratory, NASA, Pasadena, CA • 2011, Department of Microbiology, Montana State University, Bozeman, MT • 2011, Geo-club seminar, Division of Geological & Planetary Sciences, Caltech, Pasadena, CA • 2010, Institute of Biophysical Chemistry, University of Vienna, Austria • 2008, Centre for Marine Bio-Innovation, University of New South Wales, Sydney, Australia

Contributed communications • since 2007, 9 contributed talks at international conferences or workshops • since 2007, 16 first author poster presentations at international conferences or workshops

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Roland Hatzenpichler Curriculum vitae

Scholarships, travel grants, and poster awards totaling $12,000 • 2012, travel award by the International Society for Microbial Ecology, ISME • 2010, scholarship award by the Gordon and Betty Moore foundation • 2009, scholarship award by the Gordon and Betty Moore foundation • 2009, scholarship award by the University of Vienna, Austria • 2009, scholarship award by the Austrian federal state Styria • 2008, travel award by the International Society for Microbial Ecology, ISME • 2008, travel award of the University of Vienna, Austria • 2007, poster award of the Thermophiles conference. Bergen, Norway • 2005-2007, three travel awards by the European Astrobiology Network Association, EANA

Synergistic activities • since 2015, member of the Editorial board of Frontiers in Microbiology, section Microbial Physiology and Metabolism • 2015, co-convener of plenary session “(R)evolutionary cross-talks between microbes and planet Earth” at the ASM General Meeting, New Orleans, LA (upcoming) • since 2014, member of Junior Advisory Group of the American Society for Microbiology • regular ad hoc reviewer for the journals The ISME Journal, Environmental Microbiology, Environmental Microbiology Reports, Applied and Environmental Microbiology, Frontiers in Microbiology, FEMS Microbiology Ecology, PLoSOne, Microbiology, Scientific Reports, Antonie van Leeuwenhoek Journal of Microbiology, Environmental Science and Technology • external reviewer for grant applications to NASA’s Exobiology & Evolutionary Biology Program, NASA’s Earth and Space Sciences Graduate Fellowship program, and the French National Research Agency • reviewer for a book chapter on techniques to study single cell ecophysiology • 2009, Organizer of the 7th International workshop on New Techniques In Microbial Ecology (INTIME-7). 50 participants from six institutions in Austria, Denmark, Germany, Great Britain, and the US presented 29 talks on different topics in microbial ecology.

Teaching experience • Advisor and mentor of undergraduates, PhD students, and guest scientists: ◦ Teresa Mayer, master student at Jet Propulsion Laboratory, JPL (since 2014); topic: detection of microbial cells in NASA spacecraft assembly and space simulation rooms ◦ Grayson Chadwick, formerly undergraduate student, now graduate student at Caltech (since 2011); topics: diversity and activity of methane-cycling microorganisms; multicellular magnetotactic bacteria; click chemistry amino acid tagging of ANME-SRB consortia ◦ Priscilla Miranda, master student at Cal State Long Beach (since 2013); topic: visualization and co-localization analyses of cells involved in sulfur-cycling in microbial mats ◦ Cody Finke, graduate student at Caltech (2014); topic: visualization of in situ anabolic activity in psychrophilic archaea and bacteria via click chemistry amino acid tagging ◦ Julia Brown, undergraduate student at Caltech (2013-2014); topic: click chemistry mediated amino acid tagging of bacteriophages ◦ Kristen Brileya, visiting graduate student from Montana State University, Bozeman (2010); topic: FISH-detection of sulfate reducers and methanogens in microbial biofilms

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Roland Hatzenpichler Curriculum vitae

◦ Nathalie Schuster, master student at DOME, University of Vienna, Austria (2008-2009); topic: cultivation, diversity analysis, and physiological characterization of ammonia-and nitrite-oxidizing microbes, with an emphasis on the characterization of N. gargensis ◦ Gilbert Flores, visiting graduate student from Portland State University, OR (2008); topic: in situ detection of anaerobic archaea in deep sea hydrothermal vent chimneys ◦ Michael Cunliffe, visiting postdoc from the University of Warwick, Coventry, UK (2006); topics: in situ activity studies of lake microbes via FISH-MAR ◦ Two high school students; both performed 1.5 month summer internships at DOME, University of Vienna (2005, 2007) • 2007-2011, Supervisor, co-organizer and lecturer in the annual International FISH course, aimed at graduate student and postgraduate level (20 hours seminar and 35 hours lab). DOME, University of Vienna, Austria • 2007-2010, Supervisor and co-organizer of the annual 2-week undergraduate student course Identification of uncultured microorganisms (24 hours class and 60 hours lab). DOME, University of Vienna, Austria

Public outreach • 2012-2013, Chair and judge of the Senior microbiology section of the 62nd - 63th annual Los Angeles County Science & Engineering Fair • 2013, Carl Woese, (R)evolutionary Biologist. Blog article for Wired magazine • 2009, participant and finalist in the Austrian Fame Lab public communication of science competition which culminated in a public talk (audience of ~400) on microbial ecology (You are never alone!) hosted by the Technical Museum of Vienna, Austria • 2008, Microorganisms - the unseen majority. Public talk on environmental microbiology, hosted by the Austrian Academy of Sciences (audience of ~50). Vienna, Austria • 2005, Genetic engineering. Yesterday, today, tomorrow. Two public talks (audience of ~60)

Professional memberships • American Society for Microbiology (ASM) • International Society for Microbial Ecology (ISME) • Austrian Scientists and Scholars in North America (ASCINA)

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Roland Hatzenpichler Curriculum vitae

List of publications

>1,000 citations (source: GoogleScholar) equal contribution, *corresponding author

Peer reviewed • Hatzenpichler R* and Orphan VJ*. Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT). Book chapter for Hydrocarbon and Lipid Microbiology Protocols, Vol. 7: Single-cell and single-molecule methods. Springer Protocols Handbooks, doi 10.1007/8623_2015_61 (2015) • Tavormina PL, Hatzenpichler R, McGlynn S, Chadwick G, Dawson K, Connon S, and Orphan VJ. Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the Deep Sea 1 clade of marine methanotrophs. Int J Syst Evo Microbiol, 65: 251–259 (2015) • Hatzenpichler R*, Scheller S, Tavormina PL, Babin B, Tirrell D, and Orphan VJ*. In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry. Environ Microbiol, 16: 2568-2590 (2014) ► Cover article ► Discussed in a “Research Highlight” • Ma L, Kim J, Hatzenpichler R, Karymov MA, Hubert N, Hanan IM, Chang EB, and Ismagilov RF. Gene-targeted microfluidic cultivation validated by isolation of a gut bacterium listed in Human Microbiome Project’s Most Wanted taxa. Proc Natl Acad Sci USA, 111: 9768–9773 (2014) • Lebedeva EV, Hatzenpichler R, Pelletier E, Schuster N, Hauzmayer S, Bulaev A, Grigorjeva NV, Galushko A, Schmid M, Palatinsky M, Le Paslier D, Daims H, and Wagner M. Enrichment and genome sequence of the group I.1a ammonia-oxidizing archaeon “Ca. Nitrosotenuis uzonensis” representing a clade globally distributed in thermal habitats. PLoSOne, 8: e80835 (2013) (equal contribution) • Spang A, Poehlein A, Offre P, Zumbrägel S, Haider S, Rychlik N, Nowka B, Schmeisser C, Lebedeva E, Rattei T, Bӧhm C, Schmid M, Galushko A, Hatzenpichler R, Weinmaier T, Daniel R, Schleper C, Spieck E, Streit W, and Wagner M. The genome of the ammonia- oxidizing Candidatus Nitrososphaera gargensis: Insights into metabolic versatility and environmental adaptations. Environ Microbiol, 14: 3122-3145 (2012) • Hatzenpichler R*. Diversity, physiology, and niche differentiation of ammonia-oxidizing archaea. Appl Environ Microbiol, 78: 7501-7510 (2012) ► Review article • Mußmann M, Brito I, Pitcher A, Damsté JS, Hatzenpichler R, Richter A, Nielsen JL, Nielsen P H, Müller A, Daims H, Wagner M, and Head IM. Thaumarchaeotes abundant in refinery nitrifying sludges express amoA but are not obligate autotrophic ammonia oxidizers. Proc Natl Acad Sci USA, 108: 16771-16776 (2011) • Shapiro OH, Hatzenpichler R*, Buckley DH, Zinder SH, and Orphan VJ. Multicellular photo-magnetotactic bacteria. Environ Microbiol Rep, 3: 233-238 (2011) (equal contribution) ► Chief Editor’s Choice Article of 2011

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Roland Hatzenpichler Curriculum vitae

• Spang A, Hatzenpichler R, Brochier-Armanet C, Rattei T, Tischler P, Spieck E, Streit W, Stahl DA, Wagner M, and Schleper C. Distinct gene set in two different lineages of ammonia- oxidizing archaea supports the phylum Thaumarchaeota. Trends Microbiol 18:331-40 (2010) ► Cover article • Hatzenpichler R, Lebedeva EV, Spieck E, Stoecker K, Richter A, Daims H, and Wagner M. A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring. Proc Natl Acad Sci USA, 105: 2134-2139 (2008)

In preparation • Hatzenpichler R*, Goudeau D, Chadwick G, Malmstrom R, Woyke T, Orphan VJ. Translational activity-based sorting of uncultured microbes enabled by bioorthogonal non- canonical amino acid tagging (BONCAT). In prep. for Proc Natl Acad Sci USA • Miranda P, Thao M, Martinez C, Hatzenpichler R, Orphan VJ, Stevens L, and Dillon J. Diversity and activity of sulfur-cycling chemoautotrophic microbial mats in the Palos Verdes hydrothermal vent field. In prep. for Appl Environ Microbiol • Haider S, Stoecker K, Hoshino T, Hatzenpichler R, Bendinger B, and Wagner M. Metagenomics and in situ RCA-FISH reveal that some Crenothrix polyspora strains possess a conventional particulate methane monooxygenase. In prep. for Environ Microbiol

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