Efficient Professional Protein and Antibody Platforms THAP1 Antibody Basic information: Catalog No.: UMA20969 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal 8D10C8 Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: The antibody was purified by immunogen affinity chromatography. Useful Information: WB:1:500 - 1:2000 ICC:1:200 - 1:1000 Applications: FCM:1:200 - 1:400 ELISA:1:10000 Reactivity: Human Specificity: This antibody recognizes THAP1 protein. Purified recombinant fragment of human THAP1 (AA: 1-213) expressed in E. Immunogen: Coli. The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and Description: functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Uniprot: Q9NVV9 BiowMW: 24.9kDa Buffer: Purified antibody in PBS with 0.05% sodium azide Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Figure 2:Western blot analysis using THAP1 mAb against human THAP1 (AA: 1-213) recombinant protein. (Expected MW is 50.3 kDa)

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Figure 3:Western blot analysis using THAP1 mAb against HEK293 (1) and THAP1 (AA: 1-213)-hIgGFc transfected HEK293 (2) cell lysate.

Figure 4:Immunofluorescence analysis of Hela cells using THAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phal- loidin. Secondary antibody from Fisher (Cat#: 35503)

Figure 5:Flow cytometric analysis of Jurkat cells using THAP1 mouse mAb (green) and negative control (red).

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]