THAP1 Antibody

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Efficient Professional Protein and Antibody Platforms THAP1 Antibody Basic information: Catalog No.: UMA20969 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal 8D10C8 Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: The antibody was purified by immunogen affinity chromatography. Useful Information: WB:1:500 - 1:2000 ICC:1:200 - 1:1000 Applications: FCM:1:200 - 1:400 ELISA:1:10000 Reactivity: Human Specificity: This antibody recognizes THAP1 protein. Purified recombinant fragment of human THAP1 (AA: 1-213) expressed in E. Immunogen: Coli. The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and Description: functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Uniprot: Q9NVV9 BiowMW: 24.9kDa Buffer: Purified antibody in PBS with 0.05% sodium azide Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Figure 2:Western blot analysis using THAP1 mAb against human THAP1 (AA: 1-213) recombinant protein. (Expected MW is 50.3 kDa) Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Figure 3:Western blot analysis using THAP1 mAb against HEK293 (1) and THAP1 (AA: 1-213)-hIgGFc transfected HEK293 (2) cell lysate. Figure 4:Immunofluorescence analysis of Hela cells using THAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phal- loidin. Secondary antibody from Fisher (Cat#: 35503) Figure 5:Flow cytometric analysis of Jurkat cells using THAP1 mouse mAb (green) and negative control (red). Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
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Structural Basis for the Regulatory Interactions of Proapoptotic Par-4
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THAP1 Conjugated Antibody
Product Datasheet THAP1 Conjugated Antibody Catalog No: #C48420 Package Size: #C48420-AF350 100ul #C48420-AF405 100ul #C48420-AF488 100ul Orders: [email protected] Support: [email protected] #C48420-AF555 100ul #C48420-AF594 100ul #C48420-AF647 100ul #C48420-AF680 100ul #C48420-AF750 100ul #C48420-Biotin 100ul Description Product Name THAP1 Conjugated Antibody Host Species Mouse Clonality Monoclonal Species Reactivity Hu Immunogen Description Recombinant protein Conjugates Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750 Other Names 4833431A01Rik antibody DYT6 antibody FLJ10477 antibody MGC33014 antibody Nuclear proapoptotic factor antibody THAP 1 antibody THAP domain containing 1 antibody THAP domain containing apoptosis associated protein 1 antibody THAP domain containing protein 1 antibody THAP domain protein 1 antibody THAP domain-containing protein 1 antibody Thap1 antibody THAP1_HUMAN antibody Accession No. Swiss-Prot#:Q9NVV9 Calculated MW 25 kDa Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide Storage Store at 4°C in dark for 6 months Application Details Suggested Dilution: AF350 conjugated: most applications: 1: 50 - 1: 250 AF405 conjugated: most applications: 1: 50 - 1: 250 AF488 conjugated: most applications: 1: 50 - 1: 250 AF555 conjugated: most applications: 1: 50 - 1: 250 AF594 conjugated: most applications: 1: 50 - 1: 250 AF647 conjugated: most applications: 1: 50 - 1: 250 AF680 conjugated: most applications: 1: 50 - 1: 250 AF750 conjugated: most applications: 1: 50 - 1: 250 Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000 Background THAP1 (THAP domain containing, apoptosis associated protein 1) is a 213 amino acid protein that localizes to the nucleoplasm and contains one THAP-type zinc finger, a conserved DNA-binding domain.
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Tan et al. Cancer Cell Int (2020) 20:598 https://doi.org/10.1186/s12935-020-01704-y Cancer Cell International PRIMARY RESEARCH Open Access Expression of PAWR predicts prognosis of ovarian cancer Jiahong Tan1,2, Kangjia Tao1,2, Xu Zheng1,2, Dan Liu1,2, Ding Ma1,2 and Qinglei Gao1,2* Abstract Background: Ovarian cancer greatly threatens the general health of women worldwide. Implementation of predic- tive prognostic biomarkers aids in ovarian cancer management. Methods: Using online databases, the general expression profle, target-disease associations, and interaction net- work of PAWR were explored. To identify the role of PAWR in ovarian cancer, gene correlation analysis, survival analysis, and combined analysis of drug responsiveness and PAWR expression were performed. The predictive prognostic value of PAWR was further validated in clinical samples. Results: PAWR was widely expressed in normal and cancer tissues, with decreased expression in ovarian cancer tissues compared with normal tissues. PAWR was associated with various cancers including ovarian cancer. PAWR formed a regulatory network with a group of proteins and correlated with several genes, which were both implicated in ovarian cancer and drug responsiveness. High PAWR expression denoted better survival in ovarian cancer patients (OS: HR 0.84, P 0.0077; PFS, HR 0.86, P 0.049). Expression of PAWR could predict platinum responsiveness in ovarian =cancer and= there was a positive= correlation= between PAWR gene efect and paclitaxel sensitivity. In 12 paired clinical samples, the cancerous tissues exhibited signifcantly lower PAWR expression than matched normal fallopian tubes. The predictive prognostic value of PAWR was maintained in a cohort of 50 ovarian cancer patients.
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THAP1 Gene THAP Domain Containing 1
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A Woman with a Novel Mutation of THAP1 with a Prominent Response to Deep Brain Stimulation of the Globus Pallidus Internus
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STAT3 Targets Suggest Mechanisms of Aggressive Tumorigenesis in Diffuse Large B Cell Lymphoma
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Downloaded from molpharm.aspetjournals.org at ASPET Journals on September 28, 2021 1 This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted. The final version may differ from this version. This article has not been copyedited and formatted.
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