DOCSLIB.ORG

Design and Construction of Designer Bioester Libraries for Validation of the Modular Cell Theory

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Design and Construction of Designer Bioester Libraries for Validation of the Modular Cell Theory University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 12-2016 Design and Construction of Designer Bioester Libraries for Validation of the Modular Cell Theory Donovan Layton University of Tennessee, Knoxville, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Recommended Citation Layton, Donovan, "Design and Construction of Designer Bioester Libraries for Validation of the Modular Cell Theory. " PhD diss., University of Tennessee, 2016. https://trace.tennessee.edu/utk_graddiss/4101 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Donovan Layton entitled "Design and Construction of Designer Bioester Libraries for Validation of the Modular Cell Theory." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Doctor of Philosophy, with a major in Chemical Engineering. Cong T. Trinh, Major Professor We have read this dissertation and recommend its acceptance: Gladys Alexandre, Eric Boder, Paul Dalhaimer Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Design and Construction of Designer Bioester Libraries for Validation of the Modular Cell Theory A Dissertation Presented for the Doctor of Philosophy Degree The University of Tennessee, Knoxville Donovan Layton December 2016 Copyright © 2016 by Donovan S. Layton All rights reserved. ii Dedicated to my parents Mitch and Sherry and my grandparents Barbra, Betty, Jay and Sam iii Acknowledgements First, I would like to thank and praise my savior, Jesus Christ. Without him, none of this would be possible. Second, I would like to immensely thank my advisor, Dr. Cong T. Trinh, for all of the guidance and insight that has been instrumental to my research. The stimulating discussion and teachings have allowed me to pursue some of my crazy ideas and develop interesting techniques. I would not be writing this otherwise. I would like to thank my committee members, Gladys Alexandre, Eric Boder, Paul Dalhaimer and my collaborators, Fu-Min Menn and Adam Guss for their discussions and support for my PhD. I am immensely grateful for Amy Brewer, Rita Gray, Amber Tipton and Jennifer Wolfenbarger for their general guidance and help with ordering my research materials. I am additionally very grateful for the Department of Chemical and Biomolecular Engineering at the University of Tennessee and the BioEnergy Science Center at Oak Ridge National Lab for admitting me to the program and their continual support for my research. I am immensely grateful for Laura Jarboe for sparking my initial interest in pursuing my graduate degree and passion for research at Iowa State University. I would additionally like to thank both Elsevier and Wiley publishing groups for publishing my works in this dissertation. I would specifically like to thank Tyler Bennett, Brian Fane, R. Adam Thompson, and Michael Wierzbicki for lending their ear at any time of the day, sometimes at 3 am, discussing all ideas, and for their great friendship. The countless conversations, ideas, and iv guidance throughout my graduate research would not be completed without them. I would like to thank my closest friends from home and Iowa State University for their friendship and their overwhelming support and interest in my research, in particular Emily Davenport, Greg Goin, Cody Hopkins, KC Keim, Ryan Kincade, Chuck Light, Brett Mech, Cody Moore, Jake O’Brien, Jarred O’Brien, and Mark Spero. I would additionally like to thank my closest friends I have developed at the University of Tennessee for their friendship, support, ideas and discussions, in particular, Rob Atkinson, Alex Meyers, Beth Conerty. I would also like to thank my lab mates for their friendship, support, discussions and for smelling my bacterial cultures, validating I wasn’t crazy, in particular Lorenzo Briganti, D.J. Conner, Sergio Garcia, Julie Hipp, Drew Kirkpatrick, Jong-Won Lee, Katie Lutes, Brian Mendoza, Dr. Paulo Avilo Neto, Dr. Narayan Niraula, Dr. Seunghyun Ryu, Kevin Spellman, Caleb Walker, Brandon Wilbanks, and Akshitha Yarrabothoula. I would also like to further thank Brandon Wilbanks for all of the help and discussions with various aspects of my research and for making sacrifices to take time points in the middle of the night. I could not have done it all without him. Finally, I would like to thank my family members including grandparents, aunts, uncles, cousins, siblings, and parents for all of their unfathomable encouragement love, kindness, and support. I would have never accomplished this work without them. I am grateful for my sisters Shannon and Allyson for all of their laughs and phone calls always checking in with me. I am also grateful for my parents Mitch and Sherry who without their instilled drive, fight, and determination, I would not be here today. I love you all. v Abstract Renewable and sustainable fuels and chemicals are required for mankind to reduce their dependence on petroleum. Metabolic engineering and synthetic biology have provided avenues for production of renewable fuels and chemicals by using waste feedstocks derived from biomass, municipal, and off gases, such as carbon dioxide and methane, using microbial cell factories. However, development of optimal microbial cell factories has been a challenge due to the vast combinations of pathways, genetic parts, and hosts to produce a targeted product. The purpose of this work is for validation of the modular cell theory via rational pathway design and testing for development of optimal microbial cell factories. This dissertation is divided up into four different parts. Part I focuses on engineering and production of butyrate ester libraries for use as fuels, flavors, fragrances and solvents, specifically ethyl butyrate, isopropyl butyrate, and isobutyl butyrate using a modular chassis cell derived from the modular cell theory. Part II focuses on the synthesis of designer esters from waste organic acids, the carboxylates, as well as characterizing the enzyme responsible for condensing ester precursor molecules for novel activity using the modular chassis cell. Part III focuses on the expansion of part II by modulating an ester precursor molecule for the production of novel esters that can be used as next generation biofuels. Part IV focuses on further validating the modular cell theory by using growth-based selection for ethanol production by varying open reading frames and genetic parts. The work presented will validate and further provide insights to modular cell theory and ester biosynthesis from fermentable sugars and organic acids. vi Table of Contents 1 Introduction ................................................................................................................1 1.1 References ............................................................................................................ 8 2 Engineering Modular Ester Fermentative Pathways in Escherichia coli ............15 2.1 Abstract .............................................................................................................. 16 2.2 Introduction ........................................................................................................ 17 2.2.1 Materials and Methods ................................................................................ 21 2.2.2 Strain construction ...................................................................................... 21 2.2.3 Plasmid/pathway construction .................................................................... 21 2.2.3.1 Construction of the butyryl-CoA submodule ...................................... 26 2.2.3.2 Construction of the butyryl-CoA plus AAT submodule ..................... 27 2.2.3.3 Construction of the ethanol production submodule ............................. 27 2.2.3.4 Construction of the isopropanol production submodule ...................... 28 2.2.3.5 Construction of the isobutanol production submodule. ....................... 28 2.2.4 Medium and cell culturing techniques ........................................................ 29 2.2.4.1 Culture media ...................................................................................... 29 2.2.4.2 Strain characterization for ester production ......................................... 29 2.2.5 Analytical methods ..................................................................................... 31 2.2.5.1 High performance liquid chromatography (HPLC)............................. 31 2.2.5.2 Gas chromatography coupled with mass spectroscopy (GC/MS) ....... 31 2.3 Results ................................................................................................................ 32 2.3.1 Establishing the butyrate ester fermentative pathways in E. coli ............... 32 2.3.1.1 Investigating targeted butyrate ester production by alcohol addition .. 33 2.3.1.2 Demonstrating extracellular secretion of butyrate esters .................... 35 2.3.1.3 Engineering E. coli base strain
Load more

Recommended publications
  • Retention Indices for Frequently Reported Compounds of Plant Essential Oils
    Retention Indices for Frequently Reported Compounds of Plant Essential Oils V. I. Babushok,a) P. J. Linstrom, and I. G. Zenkevichb) National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA (Received 1 August 2011; accepted 27 September 2011; published online 29 November 2011) Gas chromatographic retention indices were evaluated for 505 frequently reported plant essential oil components using a large retention index database. Retention data are presented for three types of commonly used stationary phases: dimethyl silicone (nonpolar), dimethyl sili- cone with 5% phenyl groups (slightly polar), and polyethylene glycol (polar) stationary phases. The evaluations are based on the treatment of multiple measurements with the number of data records ranging from about 5 to 800 per compound. Data analysis was limited to temperature programmed conditions. The data reported include the average and median values of retention index with standard deviations and confidence intervals. VC 2011 by the U.S. Secretary of Commerce on behalf of the United States. All rights reserved. [doi:10.1063/1.3653552] Key words: essential oils; gas chromatography; Kova´ts indices; linear indices; retention indices; identification; flavor; olfaction. CONTENTS 1. Introduction The practical applications of plant essential oils are very 1. Introduction................................ 1 diverse. They are used for the production of food, drugs, per- fumes, aromatherapy, and many other applications.1–4 The 2. Retention Indices ........................... 2 need for identification of essential oil components ranges 3. Retention Data Presentation and Discussion . 2 from product quality control to basic research. The identifi- 4. Summary.................................. 45 cation of unknown compounds remains a complex problem, in spite of great progress made in analytical techniques over 5.
    [Show full text]
  • Effect of Enzymes on Strawberry Volatiles During Storage, at Different Ripeness
    Effect of Enzymes on Strawberry Volatiles During Storage, at Different Ripeness Level, in Different Cultivars and During Eating Thesis Presented in Partial Fulfillment of the Requirements for the Degree Master of Science in the Graduate School of The Ohio State University By Gulsah Ozcan Graduate Program in Food Science and Technology The Ohio State University 2010 Thesis Committee: Sheryl Ann Barringer, Adviser W. James Harper John Litchfield 1 Copyright by Gülşah Özcan 2010 ii ABSTRACT Strawberry samples with enzyme activity and without enzyme activity (stannous chloride added) were measured for real time formation of lipoxygenase (LOX) derived aroma compounds after 5 min pureeing using selected ion flow tube mass spectrometry (SIFT-MS). The concentration of (Z)-3-hexenal and (E)-2-hexenal increased immediately after blending and gradually decreased over time while hexanal concentration increased for at least 5 min in ground strawberries. The formation of hexanal was slower than the formation of (Z)-3-hexenal and (E)-2-hexenal in the headspace of pureed strawberries. The concentration of LOX aldehydes and esters significantly increased during refrigerated storage. Damaging strawberries increased the concentration of LOX aldehydes but did not significantly affect the concentration of esters. The concentrations of many of the esters were strongly correlated to their corresponded acids and/or aldehydes. The concentration of LOX generated aldehydes decreased during ripening, while fruity esters increased. Different varieties had different aroma profiles and esters were the greatest percentage of the volatiles. The aroma release of some of the LOX derived aldehydes in the mouthspace in whole strawberries compared to chopped strawberries showed that these volatiles are formed in the mouth during chewing.
    [Show full text]
  • Expanding the Modular Ester Fermentative Pathways for Combinatorial Biosynthesis of Esters from Volatile Organic Acids
    ARTICLE Expanding the Modular Ester Fermentative Pathways for Combinatorial Biosynthesis of Esters From Volatile Organic Acids Donovan S. Layton,1,2 Cong T. Trinh1,2,3 1 Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 2 BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 3 Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, Tennessee; telephone: þ865-974-8121; fax: 865-974-7076; e-mail: [email protected] Biotechnol. Bioeng. 2016;113: 1764–1776. ABSTRACT: Volatile organic acids are byproducts of fermentative ß 2016 Wiley Periodicals, Inc. metabolism, for example, anaerobic digestion of lignocellulosic KEYWORDS: modular chassis cell; carboxylate; ester; acyl acetate; biomass or organic wastes, and are often times undesired inhibiting acyl acylate; ester fermentative pathway cell growth and reducing directed formation of the desired products. Here, we devised a general framework for upgrading these volatile organic acids to high-value esters that can be used as flavors, fragrances, solvents, and biofuels. This framework employs the acid-to-ester modules, consisting of an AAT (alcohol Introduction acyltransferase) plus ACT (acyl CoA transferase) submodule and an alcohol submodule, for co-fermentation of sugars and organic Harnessing renewable or waste feedstocks (e.g., switchgrass, corn acids to acyl CoAs and alcohols to form a combinatorial library of stover, agricultural residue, or municipal solid waste)
    [Show full text]
  • Reuse of Oak Chips for Modification of the Volatile Fraction of Alcoholic Beverages
    LWT - Food Science and Technology 135 (2021) 110046 Contents lists available at ScienceDirect LWT journal homepage: www.elsevier.com/locate/lwt Reuse of oak chips for modification of the volatile fraction of alcoholic beverages Eduardo Coelho *, Jos´e A. Teixeira , Teresa Tavares , Lucília Domingues , Jos´e M. Oliveira CEB – Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal ARTICLE INFO ABSTRACT Keywords: New or used barrels can be applied in ageing of alcoholic beverages. Compounds adsorbed in wood migrate Wood ageing between beverages along with wood extractives. As barrel ageing is costly and time-consuming, processes using Barrel alternatives wood fragments have been gaining interest. These generate wood residues for which the reuse is still not well Volatile compounds established. This work aims at the reuse of oak fragments for the additive ageing of alcoholic beverages. Oak Sensory properties chips, previously immersed in fortifiedwine, were applied to beer, wine and grape marc spirit. Wood compounds and adsorbed wine volatiles were extracted, with more impact and satisfactory yields on beer composition. Also, wood adsorbed beverages compounds in a subtractive ageing phenomena. Beer formulations using different binomial wood concentration/temperature combinations were generated and presented to trained tasters. Higher temperatures and wood concentrations led to prominence of wood descriptors and lower perception of fruity and floral aromas, reflecting the changes in chemical composition. 1. Introduction several other (Mosedale & Puech, 2003). During its lifecycle, cooperage wood can be used, reused, regenerated and eventually discarded. For Wood ageing is commonly used as a strategy to modify and enhance barrels, there is a well-established reuse flow depending on the aged the composition of alcoholic beverages.
    [Show full text]
  • Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida Rugosa Lipase Immobilized in Microemulsion Based Organogels: Effect of Parameters and Reusability
    Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 353845, 14 pages http://dx.doi.org/10.1155/2014/353845 Research Article Biocatalytic Synthesis of Flavor Ester ‘‘Pentyl Valerate’’ Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels: Effect of Parameters and Reusability Tripti Raghavendra,1 Nilam Panchal,1 Jyoti Divecha,2 Amita Shah,1 and Datta Madamwar1 1 BRD School of Biosciences, Sardar Patel Maidan, Sardar Patel University, Satellite Campus, Vadtal Road, P.O. Box 39, Vallabh Vidyanagar, Gujarat 388120, India 2 Department of Statistics, Sardar Patel University, Vallabh Vidyanagar, Gujarat 388120, India Correspondence should be addressed to Datta Madamwar; datta [email protected] Received 28 February 2014; Revised 5 May 2014; Accepted 19 May 2014; Published 1 July 2014 Academic Editor: Yunjun Yan Copyright © 2014 Tripti Raghavendra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Pentyl valerate was synthesized biocatalytically using Candida rugosa lipase (CRL) immobilized in microemulsion based organogels ∘ (MBGs). The optimum conditions were found to be pH 7.0, temperature of 37 C, ratio of concentration of water to surfactant (Wo) of 60, and the surfactant sodium bis-2-(ethylhexyl)sulfosuccinate (AOT) for MBG preparation. Although kinetic studies revealed that the enzyme in free form had high affinity towards substrates ( = 23.2 mM for pentanol and 76.92 mM for valeric acid) whereas, after immobilization, the values increased considerably (74.07 mM for pentanol and 83.3 mM for valeric acid) resulting in a slower reaction rate, the maximum conversion was much higher in case of immobilized enzyme (∼99%) as compared to free enzyme (∼19%).
    [Show full text]
  • Articles Is an Important Step for the For- Mation and Transformation of Atmospheric Particles
    Atmos. Chem. Phys., 15, 5585–5598, 2015 www.atmos-chem-phys.net/15/5585/2015/ doi:10.5194/acp-15-5585-2015 © Author(s) 2015. CC Attribution 3.0 License. Compilation and evaluation of gas phase diffusion coefficients of reactive trace gases in the atmosphere: Volume 2. Diffusivities of organic compounds, pressure-normalised mean free paths, and average Knudsen numbers for gas uptake calculations M. J. Tang1, M. Shiraiwa2, U. Pöschl2, R. A. Cox1, and M. Kalberer1 1Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK 2Multiphase Chemistry Department, Max Planck Institute for Chemistry, 55128, Mainz, Germany Correspondence to: M. J. Tang ([email protected]) and M. Kalberer ([email protected]) Received: 26 January 2015 – Published in Atmos. Chem. Phys. Discuss.: 25 February 2015 Revised: 24 April 2015 – Accepted: 3 May 2015 – Published: 21 May 2015 Abstract. Diffusion of organic vapours to the surface of 1 Introduction aerosol or cloud particles is an important step for the for- mation and transformation of atmospheric particles. So far, Organic aerosols are ubiquitous in the atmosphere and can however, a database of gas phase diffusion coefficients for or- account for a dominant fraction of submicron aerosol par- ganic compounds of atmospheric interest has not been avail- ticles (Jimenez et al., 2009). Organic aerosols affect climate able. In this work we have compiled and evaluated gas phase by scattering and adsorbing solar and terrestrial radiation and diffusivities (pressure-independent diffusion coefficients) of serving as cloud condensation nuclei and ice nuclei (Kanaki- organic compounds reported by previous experimental stud- dou et al., 2005; Hallquist et al., 2009).
    [Show full text]
  • Microbial Synthesis of a Branched-Chain Ester Platform from Organic Waste Carboxylates
    Metabolic Engineering Communications 3 (2016) 245–251 Contents lists available at ScienceDirect Metabolic Engineering Communications journal homepage: www.elsevier.com/locate/mec Microbial synthesis of a branched-chain ester platform from organic waste carboxylates Donovan S. Layton a,c, Cong T. Trinh a,b,c,n a Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, The United States of America b Bredesen Center for Interdisciplinary Research and Graduate Education, The University of Tennessee, Knoxville, The United States of America c Bioenergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, The United States of America article info abstract Article history: Processing of lignocellulosic biomass or organic wastes produces a plethora of chemicals such as short, Received 6 June 2016 linear carboxylic acids, known as carboxylates, derived from anaerobic digestion. While these carbox- Received in revised form ylates have low values and are inhibitory to microbes during fermentation, they can be biologically 15 July 2016 upgraded to high-value products. In this study, we expanded our general framework for biological up- Accepted 5 August 2016 grading of carboxylates to branched-chain esters by using three highly active alcohol acyltransferases Available online 6 August 2016 (AATs) for alcohol and acyl CoA condensation and modulating the alcohol moiety from ethanol to iso- Keywords: butanol in the modular chassis cell. With this framework, we demonstrated the production of an ester Carboxylate platform library comprised of 16 out of all 18 potential esters, including acetate, propionate, butanoate, pen- Ester platform tanoate, and hexanoate esters, from the 5 linear, saturated C -C carboxylic acids.
    [Show full text]
  • Dependent Modeling Approach Derived from Semi-Empirical Quantum Mechanical Calculations
    3D-QSAR/QSPR Based Surface- Dependent Modeling Approach Derived From Semi-Empirical Quantum Mechanical Calculations 3D-QSAR/QSPR-basierter, oberflächenabhängiger Modellierungsansatz, abgeleitet von semi-empirischen quantenmechanischen Rechnungen Der Naturwissenschaftlichen Fakultät der Friedrich-Alexander-Universität Erlangen-Nürnberg Zur Erlangung des Doktorgrades Dr. rer. nat. vorgelegt von Marcel Youmbi Foka aus Kamerun Als Dissertation genehmigt von der Naturwissenschaftlichen Fakultät/ vom Fachbereich Chemie und Pharmazie der Friedrich-Alexander-Universität Erlangen-Nürnberg Tag der mündlichen Prüfung: 05.12.2018 Vorsitzender des Promotionsorgans: Prof. Dr. Georg Kreimer Gutachter/in: Prof. Dr. Tim Clark Prof. Dr. Birgit Strodel Dedication In memory of my late Mother Lucienne Metiegam, who the Lord has taken unto himself on May 3, 2009. My mother, light of my life, God rest her soul, had a special respect for my studies. She had always encouraged me to move forward. I sincerely regret the fact that today she cannot witness the culmination of this work. Maman, que la Terre de nos Ancêtres te soit légère! This is a special reward for Mr. Joseph Tchokoanssi Ngouanbe, who always supported me financially and morally. That he find here the expression of my deep gratitude. i ii Acknowledgements I would like to pay tribute to all those who have made any contribution, whether scientific or not, to help carry out this work. All my thanks go especially to Prof. Dr. Tim Clark, who gave me the opportunity and means to work in his research team. I am grateful to have had him not only supervise my work but also for his patience and for giving me the opportunity to explore this fascinating topic.
    [Show full text]
  • ENGLISH POSITIVE LIST MATERIAL (ENG).Pdf
    Attachment of Decree Number: SK15/Dir/LPPOM MUI/XI/19 HALAL POSITIVE LIST OF MATERIALS Halal Positive List of Materials consists of non critical materials, in terms of their halalness status, commonly used in prosessing industries. This material list was made based on the assessment of LPPOM MUI refer to the literature, abundance in nature, and consideration of commercially production scales. Companies that using materials listed in Halal Positive List of Materials will get advantages as follows: 1. In selection process of new materials, materials listed in Halal Positive List of Materials already get material approval from LPPOM MUI automatically prior to use. 2. In process of incoming material checking, materials listed in Halal Positive List of Materials does not require the suitability checking of the material name, manufacture's name and country of origin. 3. In product registration process, materials listed in Halal Positive List of Materials does not require supporting document. If the materials use trade name which is different with material name, the material specification document is still required. During the audit process, the auditor may check the supporting documents of material when necessary. Halal Positive List of Materials is given in the following table: Prohibited for Foods (BPOM CAS Chemical Name Regulation) Foods Cosmetics 673-84-7 2,6-Dimethyl-2,4,6-octatriene 91-57-6 2-Methylnaphthalene 1576-78-9 [(E)-hept-3-enyl] acetate 72214-23-4 7-acetoxy-3,7-dimethyl-octa-1,3- diene Page 3 of 192 2216-45-7 (4-methylphenyl)methyl
    [Show full text]
  • X International Conference “Mechanisms of Catalytic Reactions”
    Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia Lomonosov Moscow State University, Moscow, Russia 2016 X International Conference “Mechanisms of Catalytic Reactions” Svetlogorsk, Kaliningrad Region, Russia October 2 - 6, 2016 ABSTRACTS Novosibirsk-2016 Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia Lomonosov Moscow State University, Moscow, Russia X International Conference “Mechanisms of Catalytic Reactions” Svetlogorsk, Kaliningrad Region, Russia October 2 - 6, 2016 ABSTRACTS Novosibirsk-2016 УДК 544.47+66.09 ББК Г544 M45 Mechanisms of Catalytic Reactions. X International Conference (MCR-X). (October 2 - 6, 2016, Svetlogorsk, Kaliningrad Region, Russia) [Electronic resourse]: Book of abstracts / Boreskov Institute of Catalysis SB RAS ed.: prof. V.I. Bukhtiyarov, - Novosibirsk: BIC, 2016. p.328, – 1 electronic optical disc (CD-R). ISBN 978-5-906376-15-2 В надзаг.: Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia Lomonosov Moscow State University, Moscow, Russia Topics of book: – First-principles approach, theory and simulation in catalysis; – Advanced methods for studies of mechanisms of catalyzed reactions; – In-situ and operando studies of model and real catalysts; – Kinetics and reaction intermediates of catalyzed processes; – From mechanistic studies to design of advanced catalyst systems. The Conference is accompanied
    [Show full text]
  • Nicolet Vapor Phase
    Nicolet Vapor Phase Library Listing – 8,654 spectra This library is one the most comprehensive collections of vapor phase FT-IR spectra. It is an invaluable tool for scientist involved in investigations on gas phase materials. The Nicolet Vapor Phase Library contains 8654 FT-IR spectra of compounds measured in gas phase. Most spectra were acquired by the Sigma-Aldrich Co. using product samples. Additional spectra were collected by Hannover University, University of Wurzburg and Thermo Fisher Scientific applications scientists. Spectra were collected using sampling techniques including heated or room temperature gas cell or a heated light-pipe connected to the outlet of a gas chromatograph. Nicolet Vapor Phase Index Compound Name Index Compound Name 8402 ((1- 5457 (-)-8-Phenylmenthol; (-)-(1R,2S,5R)-5- Ethoxycyclopropyl)oxy)trimethylsilane Methyl-2-(2-phenyl-2-propyl)cyc 4408 (+)-1,3-Diphenylbutane 1095 (-)-Carveol, mixture of isomers; p- 4861 (+)-1-Bromo-2,4-diphenylbutane Mentha-6,8-dien-2-ol 2406 (+)-3-(Heptafluorobutyryl)camphor 3628 (-)-Diisopropyl D-tartrate 2405 (+)-3-(Trifluoroacetyl)camphor 1427 (-)-Limonene oxide, cis + trans; (-)-1,2- 281 (+)-3R-Isolimonene, trans-; (1R,4R)- Epoxy-4-isopropenyl-1-methyl (+)-p-Mentha-2,8-diene 1084 (-)-Menthol; [1R-(1a,2b,5a)]-(-)-2- 289 (+)-Camphene; 2,2-Dimethyl-3- Isopropyl-5-methylcyclohexanol methylenebicyclo[2.2.1]heptane 2750 (-)-Menthoxyacetic acid 3627 (+)-Diisopropyl L-tartrate 1096 (-)-Myrtanol, cis-; (1S,2R)-6,6- 2398 (+)-Fenchone; (+)-1,3,3- Dimethylbicyclo[3.1.1]heptane-2-metha
    [Show full text]
  • Oral Release Behavior of Wine Aroma Compounds by Using In-Mouth Headspace Sorptive Extraction (HSSE) Method
    foods Article Oral Release Behavior of Wine Aroma Compounds by Using In-Mouth Headspace Sorptive Extraction (HSSE) Method María Pérez-Jiménez, Carolina Muñoz-González and María Angeles Pozo-Bayón * Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, C/Nicolás Cabrera, 9, 28049 Madrid, Spain; [email protected] (M.P.-J.); [email protected] (C.M.-G.) * Correspondence: [email protected] Abstract: The oral release behavior of wine aroma compounds was determined by using an in-mouth headspace sorptive extraction (HSSE) procedure. For this, 32 volunteers rinsed their mouths with a red wine. Aroma release was monitored at three time points (immediately, 60 s, and 120 s) after wine expectoration. Twenty-two aroma compounds belonging to different chemical classes were identified in the mouth. Despite the large inter-individual differences, some interesting trends in oral release behavior were observed depending on the chemical family. In general, esters and linear alcohols showed rapid losses in the mouth over the three sampling times and therefore showed a low oral aroma persistence. On the contrary, terpenes, lactones, and C13 norisoprenoids showed lower variations in oral aroma release over time, thus showing a higher oral aroma persistence. Additionally, and despite their low polarity, furanic acids and guaiacol showed the highest oral aroma persistence. This work represents the first large study regarding in-mouth aroma release behavior after wine tasting, using real wines, and it confirmed that oral release behavior does not only Citation: Pérez-Jiménez, M.; depend on the physicochemical properties of aroma compounds but also on other features, such as Muñoz-González, C.; Pozo-Bayón, the molecular structure and probably, on the characteristics and composition of the oral environment.
    [Show full text]