DOCSLIB.ORG

Non Commercial Use Only

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Non Commercial Use Only Infectious Disease Reports 2016; volume 8:6320 First report of acute This complication often occurs sporadically, and in such situations, the common source of Correspondence: Enrique Rodríguez de la Rúa, postoperative endophthalmitis infection may be due to the conjunctival flora UGC Oftalmología, Hospital Universitario Virgen caused by Rothia mucilaginosa of the patient. The major pathogens are coagu- Macarena, AV/Fedriani sn, 41010 Sevilla, Spain. after phacoemulsification lase-negative staphylococci (70%), Tel.: +34.606.461164. Staphylococcus aureus (10%), streptococci E-mail: [email protected] Pablo Álvarez-Ramos,1 (9%), other Gram-positive cocci, including enterococci and mixed bacteria (5%), and Key words: Rothia mucilaginosa; endophthalmi- Amparo Del Moral-Ariza,1 tis; phacoemulsification; cataract; biofilm; Gram-negative bacilli (6%). The fact that José M. Alonso-Maroto,1 intraocular lens. Gram-positive bacteria cause >95% of the Pilar Marín-Casanova,2 cases reflect the usual pathogenesis, i.e. con- José M. Calandria-Amigueti,1 Acknowledgements: the Universitario Virgen tamination of the aqueous humor with skin Macarena Hospital is a clinical associated center Manuel Rodríguez-Iglesias,2 bacteria flora during surgery.1 However, of the Network: RETICS RD12/0034 1,3 Enrique Rodríguez de la Rúa unusual germs causing the infection are Enfermedades Oculares. “Prevención, detección 1Ophthalmology Unit, University Hospital sometimes isolated and should be suspected in precoz y tratamiento de la patología ocular preva- Puerta del Mar, Cádiz; 2Microbiology Unit, cases with a non-typical evolution. lente degenerativa y crónica”. Oftared. Instituto de Salud Carlos III. Spain. University Hospital Puerta del Mar, Cádiz; 3 Ophthalmology Unit, University Contributions: PAV obtained the data, performed Hospitals Virgen Macarena y Virgen del Case Report a literature review, and drafted the manuscript; Rocío, Sevilla, Spain JAM, AMA, JCA and ERR managed the case and revised the manuscript critically; MRI and PMC A 65 year-old female patient was urgently performed the microbiological analysis and criti- admitted to a hospital emergency room 48 cally revised the article. hours after a cataract surgery, referring to red Abstract eye and vision decrease in the operated eye. Conflict of interest: the authors declare no poten- Visual acuity on the initial exam was hands tial conflictonly of interest. We aimed at reporting the first case of rap- movement in the right eye and 20/40 in the left idly progressive acute postoperative endoph- eye. A hydrophobic acrylic aspheric intraocular Received for publication: 24 November 2015. thalmitis after phacoemulsification cataract lens was used. Ophthalmological examinations Revision received: 12 January 2016. Accepted for publication: 25 January 2016. surgery in an immunocompetent patient showed conjunctival injection in the rightuse eye, caused by Rothia mucilaginosa. An immuno- hypopyon, 3+ cells in anterior chamber and This work is licensed under a Creative Commons competent patient manifested endophthalmi- severe vitreitis with no fundus view. Acute Attribution-NonCommercial 4.0 International tis signs 48 hours after an uncomplicated post-cataract endophthalmitis was suspected. License (CC BY-NC 4.0). cataract surgery by phacoemulsification. A She was hospitalized and 23G pars plana vit- bacteria of the family Micrococcaceae was cul- rectomy was immediately performed and a vit- ©Copyright P. Álvarez-Ramos et al., 2016 tured in the vitreous biopsy, namely R. reous biopsy was taken for culture. Licensee PAGEPress, Italy mucilaginosa. The patient did not show a Additionally, she was treated with topical ocu- Infectious Disease Reports 2016; 8:6320 doi:10.4081/idr.2016.6320 favorable clinical response after vitrectomy lar applications of fortified tobramycin (15 and systemic, intravitreal, and topical fortified mg/mL) and ceftazidime (50 mg/mL) every antibiotics. The patient’s eye was very painful, hour and with intravitreal injections of van- and consequently, it deemed necessary to per- comycin (1 mg/0.1 mL) and ceftazidime (2 ceptible to all antibiotics tested (penicillins, form an evisceration. R. mucilaginosa may be mg/0.1commercial mL) after the vitrectomy and 2 and 4 cephalosporins, fluoroquinolones, erythromy- an aggressive etiologic agent for postoperative days after operation. Intravenous antibiotics cin, clindamycin, and tetracycline). endophthalmitis. Although the isolated R. (1 g of vancomycin) were also administered Although this organism was susceptible to mucilaginosa was susceptible to empirical twice a day, 500 mg ceftazidime/12 hours, as the empirical antibiotics used, a bad clinical treatment, it was impossible to control the well as the administration of systemic corticos- response was observed with increasing infection with standard treatment, Nonprobably teroid after 24 hours (oral prednisone 1 hypopyon. During the next days, pain, conjunc- due to its ability to create a biofilm around the mg/kg/day). Because of the bad evolution after tival injection, hypopyon and anterior chamber intraocular lens. 36 hours, systemic treatment was then empir- reaction worsened and a large vitreous abscess ically changed to linezolid 600 mg and moxi- was observed. The patient had no light percep- floxacin 400 mg, twice a day. tion in the eye, which was very painful. Vitreous was cultured in blood agar, choco- Evisceration was consequently performed. Introduction late agar (incubated 48h in microaerophilic conditions) and thioglycollate broth; and was Cataract surgery is one of the most common isolated in an all media pure culture of Gram- eye operations performed worldwide. Although positive cocci, forming white colonies and Discussion cataract surgery is highly effective and rela- catalase positive, which was identified by the tively safe, owing to the enormous numbers, Microbiology Laboratory as Rothia mucilagi- R. mucilaginosa, formerly called even uncommon surgical complications could nosa through matrix-assisted laser desorp- Stomatococcus mucilaginosus, is part of the be potentially harmful for many patients.1 tion/ionization-time of flight (MALDI-TOF, normal flora of the upper respiratory tract and Endophthalmitis is one of the most serious Bruker Daltonics, Bruker Corporation, oral cavity. It was reclassified into a new genus complications of cataract surgery, affecting Billerica, MA, USA). Direct Gram stain was belonging to the family Micrococcaceae in around 0.1% of the cases, and often resulting negative. Antimicrobial sensitivity test was 2000,3 based on 16S rRNA sequencing. Gram in severe visual impairment.2 done by the Kirby-Bauer method, being sus- staining reveals non-spore-forming, encapsu- [page 6] [Infectious Disease Reports 2016; 8:6320] Case Report lated Gram-positive cocci that can appear in patient could be the origin of the infection, but 2. Cao H, Zhang L, Li L, Lo S. Risk factors for pairs, tetrads, or irregular clusters. It is a fac- contamination of eye drops used in the periop- acute endophthalmitis following cataract ultative anaerobic bacterium, which grows erative period with R. mucilaginosa is also a surgery: a systematic review and meta- well on most nonselective media and in stan- possibility. Unfortunately, a search of R. analysis. PLoS One 2013;8:e71731. dard blood culture systems. On sheep blood mucilaginosa in the eye drops was not per- 3. Collins MD, Hutson RA, Båverud V, Falsen and chocolate agar, the bacterium forms clear formed when the patient was admitted in the E. Characterization of a Rothia-like organ- to gray/white, non-hemolytic, mucoid or sticky hospital. It would have been of interest to ism from a mouse: description of Rothia colonies, which adhere to the agar surface. It demonstrate the origin of the contamination. nasimurium sp. nov. and reclassification can be difficult to distinguish it from coagu- However, it was reported that R. mucilaginosa of Stomatococcus mucilaginosus as Rothia lase-negative staphylococci, micrococci, and is able to colonize a foreign body as a vascular mucilaginosa comb. nov. Int J Syst Evol streptococci based on the catalase test result. catheter.9 The organism’s ability to produce a Microbiol 2000;50:1247-51. Its inability to grow in 6.5% sodium chloride biofilm, similar to other Gram-positive bacte- 4. Ruoff KL. Miscellaneous catalase-nega- and its ability to hydrolyze gelatin and esculin ria, is believed to be a key pathogenic mecha- tive, gram-positive cocci: emerging oppor- distinguish it from species of Staphylococcus, nism. The physical protective layer provided by tunists. J Clin Microbiol 2002;40:1129-33. Micrococcus, and Enterococcus genera.4 the biofilm presumably facilitates adhesion of 5. Ramos JM, Mateo I, Rosillo EM, et al. Identification from automatic methods should the organisms to devices and renders them rel- Infection due to Rothia mucilaginosa. A correlate with phenotypic identification; other- atively refractory to medical therapy. Antibiotic respiratory pathogen? Enferm Infecc wise, genetic sequencing may be required to therapy alone is usually ineffective without Microbiol Clin 2014;32:306-9. identify this organism. It is an infrequent surgical removal of the infected device. This 6. Baeza Martínez C, Zamora Molina L, pathogen, mostly affecting immunocompro- could better support the failure of antibiotic García Sevila R, et al. Rothia mucilaginosa mized hosts. Recently, infections in immuno- treatment. Pneumonia in an immunocompetent competent hosts have been described in vari-
Load more

Recommended publications
  • Nitrate-Responsive Oral Microbiome Modulates Nitric Oxide Homeostasis and Blood Pressure in Humans
    This is an Open Access document downloaded from ORCA, Cardiff University's institutional repository: http://orca.cf.ac.uk/111622/ This is the author’s version of a work that was submitted to / accepted for publication. Citation for final published version: Vanhatalo, Anni, Blackwell, Jamie R., L'Heureux, Joanna E., Williams, David W., Smith, Ann, van der Giezen, Mark, Winyard, Paul G., Kelly, James and Jones, Andrew M. 2018. Nitrate-responsive oral microbiome modulates nitric oxide homeostasis and blood pressure in humans. Free Radical Biology and Medicine 124 , pp. 21-30. 10.1016/j.freeradbiomed.2018.05.078 filefile Publishers page: https://doi.org/10.1016/j.freeradbiomed.2018.05.07... <https://doi.org/10.1016/j.freeradbiomed.2018.05.078> Please note: Changes made as a result of publishing processes such as copy-editing, formatting and page numbers may not be reflected in this version. For the definitive version of this publication, please refer to the published source. You are advised to consult the publisher’s version if you wish to cite this paper. This version is being made available in accordance with publisher policies. See http://orca.cf.ac.uk/policies.html for usage policies. Copyright and moral rights for publications made available in ORCA are retained by the copyright holders. Free Radical Biology and Medicine 124 (2018) 21–30 Contents lists available at ScienceDirect Free Radical Biology and Medicine journal homepage: www.elsevier.com/locate/freeradbiomed Original article Nitrate-responsive oral microbiome modulates nitric oxide homeostasis and blood pressure in humans T ⁎ Anni Vanhataloa, , Jamie R. Blackwella, Joanna E.
    [Show full text]
  • INFECTIOUS DISEASES NEWSLETTER May 2017 T. Herchline, Editor LOCAL NEWS ID Fellows Our New Fellow Starting in July Is Dr. Najmus
    INFECTIOUS DISEASES NEWSLETTER May 2017 T. Herchline, Editor LOCAL NEWS ID Fellows Our new fellow starting in July is Dr. Najmus Sahar. Dr. Sahar graduated from Dow Medical College in Pakistan in 2009. She works in Dayton, OH and completed residency training from the Wright State University Internal Medicine Residency Program in 2016. She is married to Dr. Asghar Ali, a hospitalist in MVH and mother of 3 children Fawad, Ebaad and Hammad. She spends most of her spare time with family in outdoor activities. Dr Alpa Desai will be at Miami Valley Hospital in May and June, and at the VA Medical Center in July. Dr Luke Onuorah will be at the VA Medical Center in May and June, and at Miami Valley Hospital in July. Dr. Najmus Sahar will be at MVH in July. Raccoon Rabies Immune Barrier Breach, Stark County Two raccoons collected this year in Stark County have been confirmed by the Centers of Disease Control and Prevention to be infected with the raccoon rabies variant virus. These raccoons were collected outside the Oral Rabies Vaccination (ORV) zone and represent the first breach of the ORV zone since a 2004 breach in Lake County. In 1997, a new strain of rabies in wild raccoons was introduced into northeastern Ohio from Pennsylvania. The Ohio Department of Health and other partner agencies implemented a program to immunize wild raccoons for rabies using an oral rabies vaccine. This effort created a barrier of immune animals that reduced animal cases and prevented the spread of raccoon rabies into the rest of Ohio.
    [Show full text]
  • Mechanistic Model of Rothia Mucilaginosa Adaptation Toward Persistence in the CF Lung, Based on a Genome Reconstructed from Metagenomic Data
    Mechanistic Model of Rothia mucilaginosa Adaptation toward Persistence in the CF Lung, Based on a Genome Reconstructed from Metagenomic Data Yan Wei Lim1*, Robert Schmieder2, Matthew Haynes1¤, Mike Furlan1, T. David Matthews1, Katrine Whiteson1, Stephen J. Poole3, Christopher S. Hayes3,4, David A. Low3,4, Heather Maughan5, Robert Edwards2,6, Douglas Conrad7, Forest Rohwer1 1 Department of Biology, San Diego State University, San Diego, California, United States of America, 2 Computational Science Research Center, San Diego State University, San Diego, California, United States of America, 3 Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America, 4 Biomolecular Science and Engineering Program, University of California Santa Barbara, Santa Barbara, California, United States of America, 5 Ronin Institute, Montclair, New Jersey, United States of America, 6 Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, Illinois, United States of America, 7 Department of Medicine, University of California San Diego, La Jolla, California, United States of America Abstract The impaired mucociliary clearance in individuals with Cystic Fibrosis (CF) enables opportunistic pathogens to colonize CF lungs. Here we show that Rothia mucilaginosa is a common CF opportunist that was present in 83% of our patient cohort, almost as prevalent as Pseudomonas aeruginosa (89%). Sequencing of lung microbial metagenomes identified unique R. mucilaginosa strains in each patient, presumably due to evolution within the lung. The de novo assembly of a near-complete R. mucilaginosa (CF1E) genome illuminated a number of potential physiological adaptations to the CF lung, including antibiotic resistance, utilization of extracellular lactate, and modification of the type I restriction-modification system.
    [Show full text]
  • Rothia Mucilaginosa, Rarely Isolated Pathogen As an Etiological Factor of Infection of Soft Tissues in Young, Healthy Woman Roth
    ® Postepy Hig Med Dosw (online), 2013; 67: 1-5 www.phmd.pl e-ISSN 1732-2693 Case Report Received: 2012.05.30 Accepted: 2012.11.15 Rothia mucilaginosa, rarely isolated pathogen as an Published: 2013.01.11 etiological factor of infection of soft tissues in young, healthy woman Rothia mucilaginosa rzadko izolowany patogen jako czynnik etiologiczny zakażenia tkanek miękkich twarzy u młodej zdrowej kobiety Authors’ Contribution: Hanna Tomczak1A, Joanna Bilska-Stokłosa2B, Krzysztof Osmola2D, A Study Design Michał Marcinkowski2F, Wioleta Błażejewska1F, Katarzyna Myczko1F, B Data Collection 2D 2B C Statistical Analysis Bartosz Mańkowski , Iwetta Kaczmarek D Data Interpretation 1 E Manuscript Preparation Central Laboratory of Microbiology Heliodor Święcicki Clinical Hospital of the Poznań University of Medical F Literature Search Sciences, Poland 2 G Funds Collection Department of Maxillofacial Surgery Heliodor Święcicki Clinical Hospital of the Poznań University of Medical Sciences, Poland Summary This paper presents a rare case of facial soft tissue infection caused by the bacterial strain of Rothia mucilaginosa. Odontogenic background of infection and initial clinical presentation suggested the presence of typical bacterial flora and uncomplicated course of treatment. However, despite surgical intervention and broad-spectrum antibiotic therapy, the expected improvement of a cli- nical status was not achieved. Only detailed bacteriological examination allowed to establish a bacterial pathogen and start a targeted antibiotic therapy. The unusual clinical course was moni- tored by imaging CT examination and further surgical interventions. A significant improvement was obtained in the third week of hospitalization and further antibiotic therapy was continued by means of outpatient treatment. Rothia mucilaginosa infection together with dental intervention is a rare case, since most of the reports in the literature concern the patients with decreased im- munity.
    [Show full text]
  • Nitrate-Responsive Oral Microbiome Modulates Nitric Oxide Homeostasis and Blood Pressure in Humans
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Online Research @ Cardiff This is an Open Access document downloaded from ORCA, Cardiff University's institutional repository: http://orca.cf.ac.uk/111622/ This is the author’s version of a work that was submitted to / accepted for publication. Citation for final published version: Vanhatalo, Anni, Blackwell, Jamie, L'Heureux, Joanna, Williams, David, Smith, Ann, van der Giezen, Mark, Winyard, Paul, Kelly, James and Jones, Andrew 2018. Nitrate-responsive oral microbiome modulates nitric oxide homeostasis and blood pressure in humans. Free Radical Biology and Medicine 124 , pp. 21-30. 10.1016/j.freeradbiomed.2018.05.078 file Publishers page: https://doi.org/10.1016/j.freeradbiomed.2018.05.07... <https://doi.org/10.1016/j.freeradbiomed.2018.05.078> Please note: Changes made as a result of publishing processes such as copy-editing, formatting and page numbers may not be reflected in this version. For the definitive version of this publication, please refer to the published source. You are advised to consult the publisher’s version if you wish to cite this paper. This version is being made available in accordance with publisher policies. See http://orca.cf.ac.uk/policies.html for usage policies. Copyright and moral rights for publications made available in ORCA are retained by the copyright holders. 1 Nitrate-responsive oral microbiome modulates nitric oxide 2 homeostasis and blood pressure in humans 3 4 Anni Vanhatalo1, Jamie R. Blackwell1, Joanna L’Heureux1, David W. Williams2, Ann 5 Smith3, Mark van der Giezen1, Paul G.
    [Show full text]
  • Influence of the Lung Microbiome on Antibiotic Susceptibility of Cystic Fibrosis Pathogens
    LUNG SCIENCE CONFERENCE CYSTIC FIBROSIS Influence of the lung microbiome on antibiotic susceptibility of cystic fibrosis pathogens Eva Vandeplassche, Sarah Tavernier, Tom Coenye and Aurélie Crabbé Affiliation: Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium. Correspondence: Aurélie Crabbé, Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. E-mail: [email protected] @ERSpublications Interspecies interactions in the lung microbiome may influence the outcome of antibiotic treatment targeted at cystic fibrosis pathogens http://bit.ly/2WQp0iQ Cite this article as: Vandeplassche E, Tavernier S, Coenye T, et al. Influence of the lung microbiome on antibiotic susceptibility of cystic fibrosis pathogens. Eur Respir Rev 2019; 28: 190041 [https://doi.org/ 10.1183/16000617.0041-2019]. ABSTRACT The lungs of patients with cystic fibrosis (CF) are colonised by a microbial community comprised of pathogenic species, such as Pseudomonas aeruginosa and Staphylococcus aureus, and microorganisms that are typically not associated with worse clinical outcomes (considered as commensals). Antibiotics directed at CF pathogens are often not effective and a discrepancy is observed between activity of these agents in vitro and in the patient. This review describes how interspecies interactions within the lung microbiome might influence the outcome of antibiotic treatment targeted at common CF pathogens. Protective mechanisms by members of the microbiome such as antibiotic degradation (indirect pathogenicity), alterations of the cell wall, production of matrix components decreasing antibiotic penetration, and changes in metabolism are discussed. Interspecies interactions that increase bacterial susceptibility are also addressed. Furthermore, we discuss how experimental conditions, such as culture media, oxygen levels, incorporation of host–pathogen interactions, and microbial community composition may influence the outcome of microbial interaction studies related to antibiotic activity.
    [Show full text]
  • Identification of Staphylococcus Species, Micrococcus Species and Rothia Species
    UK Standards for Microbiology Investigations Identification of Staphylococcus species, Micrococcus species and Rothia species This publication was created by Public Health England (PHE) in partnership with the NHS. Identification | ID 07 | Issue no: 4 | Issue date: 26.05.20 | Page: 1 of 26 © Crown copyright 2020 Identification of Staphylococcus species, Micrococcus species and Rothia species Acknowledgments UK Standards for Microbiology Investigations (UK SMIs) are developed under the auspices of PHE working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk-standards-for-microbiology- investigations-smi-quality-and-consistency-in-clinical-laboratories. UK SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for- microbiology-investigations-steering-committee). The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the medical editors for editing the medical content. PHE publications gateway number: GW-634 UK Standards for Microbiology Investigations are produced in association with: Identification | ID 07 | Issue no: 4 | Issue date: 26.05.20 | Page: 2 of 26 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public
    [Show full text]
  • S41467-019-13549-9.Pdf
    ARTICLE https://doi.org/10.1038/s41467-019-13549-9 OPEN A 5700 year-old human genome and oral microbiome from chewed birch pitch Theis Z.T. Jensen 1,2,10, Jonas Niemann1,2,10, Katrine Højholt Iversen 3,4,10, Anna K. Fotakis 1, Shyam Gopalakrishnan 1, Åshild J. Vågene1, Mikkel Winther Pedersen 1, Mikkel-Holger S. Sinding 1, Martin R. Ellegaard 1, Morten E. Allentoft1, Liam T. Lanigan1, Alberto J. Taurozzi1,Sofie Holtsmark Nielsen1, Michael W. Dee5, Martin N. Mortensen 6, Mads C. Christensen6, Søren A. Sørensen7, Matthew J. Collins1,8, M. Thomas P. Gilbert 1,9, Martin Sikora 1, Simon Rasmussen 4 & Hannes Schroeder 1* 1234567890():,; The rise of ancient genomics has revolutionised our understanding of human prehistory but this work depends on the availability of suitable samples. Here we present a complete ancient human genome and oral microbiome sequenced from a 5700 year-old piece of chewed birch pitch from Denmark. We sequence the human genome to an average depth of 2.3× and find that the individual who chewed the pitch was female and that she was genetically more closely related to western hunter-gatherers from mainland Europe than hunter-gatherers from central Scandinavia. We also find that she likely had dark skin, dark brown hair and blue eyes. In addition, we identify DNA fragments from several bacterial and viral taxa, including Epstein-Barr virus, as well as animal and plant DNA, which may have derived from a recent meal. The results highlight the potential of chewed birch pitch as a source of ancient DNA.
    [Show full text]
  • Bioorthogonal Non-Canonical Amino Acid Tagging Reveals Translationally Active Subpopulations of the Cystic fibrosis Lung Microbiota
    ARTICLE https://doi.org/10.1038/s41467-020-16163-2 OPEN Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota Talia D. Valentini 1,4, Sarah K. Lucas 1,4, Kelsey A. Binder1,4, Lydia C. Cameron1, Jason A. Motl2, ✉ Jordan M. Dunitz3 & Ryan C. Hunter 1 fi 1234567890():,; Culture-independent studies of cystic brosis lung microbiota have provided few mechanistic insights into the polymicrobial basis of disease. Deciphering the specific contributions of individual taxa to CF pathogenesis requires comprehensive understanding of their ecophy- siology at the site of infection. We hypothesize that only a subset of CF microbiota are translationally active and that these activities vary between subjects. Here, we apply bioor- thogonal non-canonical amino acid tagging (BONCAT) to visualize and quantify bacterial translational activity in expectorated sputum. We report that the percentage of BONCAT- labeled (i.e. active) bacterial cells varies substantially between subjects (6-56%). We use fluorescence-activated cell sorting (FACS) and genomic sequencing to assign taxonomy to BONCAT-labeled cells. While many abundant taxa are indeed active, most bacterial species detected by conventional molecular profiling show a mixed population of both BONCAT- labeled and unlabeled cells, suggesting heterogeneous growth rates in sputum. Differ- entiating translationally active subpopulations adds to our evolving understanding of CF lung disease and may help guide antibiotic therapies targeting bacteria most likely to be susceptible. 1 Department of Microbiology & Immunology, University of Minnesota, 689 23rd Avenue SE, Minneapolis, MN 55455, United States. 2 Academic Health Center, University Flow Cytometry Resource, University of Minnesota, 6th St SE, Minneapolis, MN 55455, United States.
    [Show full text]
  • 323C950e4135eb873c5e06ee2f
    Journal of Clinical Medicine Article Characterization of Microbiota in Bronchiectasis Patients with Different Disease Severities Sang Hoon Lee 1 , YeonJoo Lee 2, Jong Sun Park 2, Young-Jae Cho 2, Ho Il Yoon 2, Choon-Taek Lee 2 and Jae Ho Lee 2,* 1 Division of Pulmonology, Department of Internal Medicine, Severance Hospital, Institute of Chest Diseases, Yonsei University College of Medicine, Seoul 120-752, Korea; [email protected] 2 Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Korea; [email protected] (Y.L.); [email protected] (J.S.P.); [email protected] (Y.-J.C.); [email protected] (H.I.Y.); [email protected] (C.-T.L.) * Correspondence: [email protected]; Tel.: +82-317-877-054 Received: 10 October 2018; Accepted: 6 November 2018; Published: 9 November 2018 Abstract: The applications of the 16S rRNA gene pyrosequencing has expanded our knowledge of the respiratory tract microbiome originally obtained using conventional, culture-based methods. In this study, we employed DNA-based molecular techniques for examining the sputum microbiome in bronchiectasis patients, in relation to disease severity. Of the sixty-three study subjects, forty-two had mild and twenty-one had moderate or severe bronchiectasis, which was classified by calculating the FACED score, based on the FEV1 (forced expiratory volume in 1 s, %) (F, 0–2 points), age (A, 0–2 points), chronic colonization by Pseudomonas aeruginosa (C, 0–1 point), radiographic extension (E, 0–1 point), and dyspnoea (D, 0–1 point).
    [Show full text]
  • Antibiotic Resistance Genes in the Actinobacteria Phylum
    European Journal of Clinical Microbiology & Infectious Diseases (2019) 38:1599–1624 https://doi.org/10.1007/s10096-019-03580-5 REVIEW Antibiotic resistance genes in the Actinobacteria phylum Mehdi Fatahi-Bafghi1 Received: 4 March 2019 /Accepted: 1 May 2019 /Published online: 27 June 2019 # Springer-Verlag GmbH Germany, part of Springer Nature 2019 Abstract The Actinobacteria phylum is one of the oldest bacterial phyla that have a significant role in medicine and biotechnology. There are a lot of genera in this phylum that are causing various types of infections in humans, animals, and plants. As well as antimicrobial agents that are used in medicine for infections treatment or prevention of infections, they have been discovered of various genera in this phylum. To date, resistance to antibiotics is rising in different regions of the world and this is a global health threat. The main purpose of this review is the molecular evolution of antibiotic resistance in the Actinobacteria phylum. Keywords Actinobacteria . Antibiotics . Antibiotics resistance . Antibiotic resistance genes . Phylum Brief introduction about the taxonomy chemical taxonomy: in this method, analysis of cell wall and of Actinobacteria whole cell compositions such as various sugars, amino acids, lipids, menaquinones, proteins, and etc., are studied [5]. (ii) One of the oldest phyla in the bacteria domain that have a Phenotypic classification: there are various phenotypic tests significant role in medicine and biotechnology is the phylum such as the use of conventional and specific staining such as Actinobacteria [1, 2]. In this phylum, DNA contains G + C Gram stain, partially acid-fast, acid-fast (Ziehl-Neelsen stain rich about 50–70%, non-motile (Actinosynnema pretiosum or Kinyoun stain), and methenamine silver staining; morphol- subsp.
    [Show full text]
  • VMB Safety Efficacy Supplement2 190619.Xlsx
    List of taxa (alphabetical order) Bacterial Phylum/Class (Order) based on NCBI taxonomy Minority group browser taxon? Abiotrophia defectiva BV Firmicutes/Bacilli (Lactobacillales) Yes Actinobacillus genus Pathobionts Gammaproteobacteria (Pasteurellales) Yes Actinomyces family BV Actinobacteria/Actinobacteria (Actinomycetales) No Actinomyces genus BV Actinobacteria/Actinobacteria (Actinomycetales) Yes Actinomyces europaeus BV Actinobacteria/Actinobacteria (Actinomycetales) Yes Actinomyces funkei BV Actinobacteria/Actinobacteria (Actinomycetales) Yes Actinomyces neuii BV Actinobacteria/Actinobacteria (Actinomycetales) No Actinomyces odontolyticus BV Actinobacteria/Actinobacteria (Actinomycetales) Yes Actinomyces turicensis BV Actinobacteria/Actinobacteria (Actinomycetales) Yes Actinomyces urogenitalis BV Actinobacteria/Actinobacteria (Actinomycetales) Yes Aerococcus genus BV Firmicutes/Bacilli (Lactobacillales) No Aerococcus christensenii BV Firmicutes/Bacilli (Lactobacillales) No Aeromonas caviae/dhakensis/ Pathobionts Gammaproteobacteria (Aeromonadales) Yes enteropelogenes/hydrophila/janda ei/taiwanensis/veronii Alistipes finegoldii/onderdonkii BV Bacteroidetes/Bacteroidia (Bacteroidales) Yes Alloiococcus genus BV Firmicutes/Bacilli (Lactobacillales) Yes Alloprevotella genus BV Bacteroidetes/Bacteroidia (Bacteroidales) No Alloprevotella rava BV Bacteroidetes/Bacteroidia (Bacteroidales) No Alloscardovia omnicolens Other Actinobacteria/Actinobacteria (Bifidobacteriales) Yes bacteria Anaerococcus genus BV Firmicutes/Tissierellia (Tissierellales)
    [Show full text]