Citrus Viroids
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Grapevine Virus Diseases: Economic Impact and Current Advances in Viral Prospection and Management1
1/22 ISSN 0100-2945 http://dx.doi.org/10.1590/0100-29452017411 GRAPEVINE VIRUS DISEASES: ECONOMIC IMPACT AND CURRENT ADVANCES IN VIRAL PROSPECTION AND MANAGEMENT1 MARCOS FERNANDO BASSO2, THOR VINÍCIUS MArtins FAJARDO3, PASQUALE SALDARELLI4 ABSTRACT-Grapevine (Vitis spp.) is a major vegetative propagated fruit crop with high socioeconomic importance worldwide. It is susceptible to several graft-transmitted agents that cause several diseases and substantial crop losses, reducing fruit quality and plant vigor, and shorten the longevity of vines. The vegetative propagation and frequent exchanges of propagative material among countries contribute to spread these pathogens, favoring the emergence of complex diseases. Its perennial life cycle further accelerates the mixing and introduction of several viral agents into a single plant. Currently, approximately 65 viruses belonging to different families have been reported infecting grapevines, but not all cause economically relevant diseases. The grapevine leafroll, rugose wood complex, leaf degeneration and fleck diseases are the four main disorders having worldwide economic importance. In addition, new viral species and strains have been identified and associated with economically important constraints to grape production. In Brazilian vineyards, eighteen viruses, three viroids and two virus-like diseases had already their occurrence reported and were molecularly characterized. Here, we review the current knowledge of these viruses, report advances in their diagnosis and prospection of new species, and give indications about the management of the associated grapevine diseases. Index terms: Vegetative propagation, plant viruses, crop losses, berry quality, next-generation sequencing. VIROSES EM VIDEIRAS: IMPACTO ECONÔMICO E RECENTES AVANÇOS NA PROSPECÇÃO DE VÍRUS E MANEJO DAS DOENÇAS DE ORIGEM VIRAL RESUMO-A videira (Vitis spp.) é propagada vegetativamente e considerada uma das principais culturas frutíferas por sua importância socioeconômica mundial. -
The Variability of Hop Latent Viroid As Induced Upon Heat Treatment
Virology 287, 349–358 (2001) doi:10.1006/viro.2001.1044, available online at http://www.idealibrary.com on View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector The Variability of Hop Latent Viroid as Induced upon Heat Treatment Jaroslav Matousˇek,* Josef Patzak,† Lidmila Orctova´,* Jo¨rg Schubert,‡ Luka´sˇ Vrba,* Gerhard Steger,§ and Detlev Riesner§,1 *Department of Molecular Genetics, Institute of Plant Molecular Biology Czech Academy of Sciences, Branisˇovska´31, 37005 Cˇ eske´Bude˘jovice, Czech Republic; †Department of Virology, Institute of Hop Research and Breeding, Kadanˇska´2525, 438 46 Zˇatec, Czech Republic; ‡Federal Centre for Breeding Research, Institute for Resistance Research and Pathogen Diagnostics, Theodor-Roemer-Weg 4, 06449 Aschersleben, Germany; and §Institute of Physical Biology, Heinrich-Heine Universita¨t Du¨sseldorf, Universita¨tsstraße 1, D-40225 Du¨sseldorf, Germany Received March 28, 2001; returned to author for revision March 30, 2001; accepted June 11, 2001; published online August 2, 2001 We have previously shown that heat treatment of hop plants infected by hop latent viroid (HLVd) reduces viroid levels. Here we investigate whether such heat treatment leads to the accumulation of sequence variability in HLVd. We observed a negligible level of mutated variants in HLVd under standard cultivation conditions. In contrast, the heat treatment of hop led to HLVd degradation and, simultaneously, to a significant increase in sequence variations, as judged from temperature gradient–gel electrophoresis analysis and cDNA library screening by DNA heteroduplex analysis. Thirty-one cDNA clones (9.8%) were identified as deviating forms. -
Field Performance of 'Marsh Seedless' Grapefruit On
582 Stuchi et al. FIELD PERFORMANCE OF ‘MARSH SEEDLESS’ GRAPEFRUIT ON TRIFOLIATE ORANGE INOCULATED WITH VIROIDS IN BRAZIL Eduardo Sanches Stuchi1,2*; Simone Rodrigues da Silva2; Luiz Carlos Donadio2; Otávio Ricardo Sempionato2; Eduardo Toller Reiff2 1 Embrapa Mandioca e Fruticultura Tropical, R. Embrapa s/n ,C.P. 7 - 44380-000 - Cruz das Almas, BA - Brasil. 2 Estação Experimental de Citricultura de Bebedouro, C.P. 74 - 14700-971 - Bebedouro, SP - Brasil. *Corresponding author <[email protected]> ABSTRACT: Some viroids reduce citrus tree growth and may be used for tree size control aiming the establishment of orchards with close tree spacing that may provide higher productivity than conventional ones. To study the effects of citrus viroids inoculation on vegetative growth, yield and fruit quality of ‘Marsh Seedless’ grapefruit (Citrus paradisi Macf.) grafted on trifoliate orange [Poncirus trifoliata (L.) Raf.], an experiment was set up in January 1991, in Bebedouro, São Paulo State, Brazil. The experimental design was randomized blocks with four treatments with two plants per plot: viroid isolates Citrus Exocortis Viroid (CEVd) + Hop stunt viroid (HSVd - CVd-II, a non cachexia variant) + Citrus III viroid (CVd-III) and Hop stunt viroid (HSVd - CVd-II, a non cachexia variant) + Citrus III viroid (CVd-III) and controls: two healthy buds (control), and no grafting (absolute control). Inoculation was done in the field, six months after planting by bud grafting. Both isolates reduced tree growth (trunk diameter, plant height, canopy diameter and volume). Trees not inoculated yielded better (average of eleven harvests) than inoculated ones but the productivity was the same after 150 months. -
Arizona Department of Agriculture Environmental & Plant Services Division 1688 W
DOUGLAS A. DUCEY MARK W. KILLIAN Governor Director Arizona Department of Agriculture Environmental & Plant Services Division 1688 W. Adams Street, Phoenix, Arizona 85007 P. (602) 542-0994 F. (602) 542-1004 SUMMARY OF EXTERIOR QUARANTINES Updated April 16, 2021 CONTACTS Jack Peterson…...…………………………………………………………………………..…Associate Director (602) 542-3575 [email protected] Rachel Paul…………………………………………………………………………...Field Operations Manager (602) 542-3243 [email protected] Jamie Legg………………………………………………………………………..Quarantine Program Manager (602) 542-0992 [email protected] INDEX Summaries………………………………………………………………………………………...……….Page 2 Nursery Stock…………………………………………………………………………...…………Page 2 House Plants……………………………………………………………………………………….Page 2 Boll Weevil Pest…………………………………………………………………………………...Page 2 Citrus Nursery Stock Pests………………………………………………………………………...Page 3 Nut Tree Pests……………………………………………………………………………………..Page 3 Nut Pests…………………………………………………………………………………………...Page 4 Lettuce Mosaic Virus……………………………………………………………………………...Page 4 Imported Fire Ants………………………………………………………………………………...Page 5 Palm Tree Pests…………………………………………………………………………………....Page 5 Noxious Weeds…………………………………………………………………………………....Page 7 Japanese beetle…………………………………………………………………………………….Page 9 Arizona Administrative Code, Title 3, Chapter 4, Article 2 Quarantine……………………..………….Page 10 April 16, 2021 www.agriculture.az.gov Page 1 SUMMARIES Nursery Stock States Regulated - All states, districts, and territories of the United States. Regulated Commodities - All trees, shrubs, vines, cacti, agaves, succulents, -
Hops – a Guide for New Growers 2017
Hops – a guide for new growers 2017 growers new – a guide for Hops Hops a guide for new growers FIRST EDITION 2017 first edition 2017 Author: Kevin Dodds www.dpi.nsw.gov.au Hops a guide for new growers Kevin Dodds Development Officer – Temperate Fruits NSW Department of Primary industries ©NSW Department of Primary Industries 2017 Published by NSW Department of Primary Industries, a part of NSW Department of Industry, Skills and Regional Development You may copy, distribute, display, download and otherwise freely deal with this publication for any purpose, provided that you attribute NSW Department of Industry, Skills and Regional Development as the owner. However, you must obtain permission if you wish to charge others for access to the publication (other than at cost); include the publication advertising or a product for sale; modify the publication; or republish the publication on a website. You may freely link to the publication on a departmental website. First published March 2017 ISBN print: 978‑1‑76058‑007‑0 web: 978‑1‑76058‑008‑7 Always read the label Users of agricultural chemical products must always read the Job number 14293 label and any permit before using the product and strictly comply with the directions on the label and the conditions of Author any permit. Users are not absolved from any compliance with Kevin Dodds, Development Officer Temperate Fruits the directions on the label or the conditions of the permit NSW Department of Primary Industries by reason of any statement made or omitted to be made in 64 Fitzroy Street TUMUT NSW 2720 this publication. -
Virus, Viroids and Mycoplasma
By: Dr. Bibha Kumari Dept. of Zoology Magadh Mahila College, Patna Email: [email protected] Virus •The viruses are non-cellular organisms. • They, in fact, have an inert crystalline structure outside the living cell. • Once they infect a cell, they take over the machinery of the host cell to replicate themselves, killing the host. •Pasteur. D.J. Ivanowsky (1892) gave the name virus. • It means venom or poisonous fluid. • According to his research, certain microbes caused the mosaic disease of tobacco. •These organisms were smaller than bacteria because they passed through bacteria-proof filters. • M.W. Beijerinek (1898) demonstrated that the extract of the infected plants of tobacco could cause infection in healthy plants. • He named the fluid as Contagium vivum fluidum (infectious living fluid). •W.M. Stanley (1935) discovered that viruses could be crystallized. These virus crystals are composed largely of proteins. •They are inert outside their specific host cell. Viruses are nothing but obligate parasites. Genetic Material of Viruses: •In addition to proteins, viruses also contain genetic material, that could be either RNA or DNA. • No virus contains both RNA and DNA. A virus is a nucleoprotein and the genetic material is infectious. •Speaking in strictly general terms, viruses infecting plants have single- stranded RNA. • On the other hand, viruses that infect animals have either single or double-stranded RNA or they might have double-stranded DNA •Bacterial viruses or bacteriophages usually have a double-stranded DNA structure. By bacteriophages, we mean viruses that infect the bacteria. • The protein coat, capsid made of small subunits (capsomeres) protects the nucleic acid. -
Hammerhead Ribozymes Against Virus and Viroid Rnas
Hammerhead Ribozymes Against Virus and Viroid RNAs Alberto Carbonell, Ricardo Flores, and Selma Gago Contents 1 A Historical Overview: Hammerhead Ribozymes in Their Natural Context ................................................................... 412 2 Manipulating Cis-Acting Hammerheads to Act in Trans ................................. 414 3 A Critical Issue: Colocalization of Ribozyme and Substrate . .. .. ... .. .. .. .. .. ... .. .. .. .. 416 4 An Unanticipated Participant: Interactions Between Peripheral Loops of Natural Hammerheads Greatly Increase Their Self-Cleavage Activity ........................... 417 5 A New Generation of Trans-Acting Hammerheads Operating In Vitro and In Vivo at Physiological Concentrations of Magnesium . ...... 419 6 Trans-Cleavage In Vitro of Short RNA Substrates by Discontinuous and Extended Hammerheads ........................................... 420 7 Trans-Cleavage In Vitro of a Highly Structured RNA by Discontinuous and Extended Hammerheads ........................................... 421 8 Trans-Cleavage In Vivo of a Viroid RNA by an Extended PLMVd-Derived Hammerhead ........................................... 422 9 Concluding Remarks and Outlooks ........................................................ 424 References ....................................................................................... 425 Abstract The hammerhead ribozyme, a small catalytic motif that promotes self- cleavage of the RNAs in which it is found naturally embedded, can be manipulated to recognize and cleave specifically -
Normes OEPP EPPO Standards
© 2004 OEPP/EPPO, Bulletin OEPP/EPPO Bulletin 34, 155 –157 Blackwell Publishing, Ltd. Organisation Européenne et Méditerranéenne pour la Protection des Plantes European and Mediterranean Plant Protection Organization Normes OEPP EPPO Standards Diagnostic protocols for regulated pests Protocoles de diagnostic pour les organismes réglementés PM 7/33 Organization Européenne et Méditerranéenne pour la Protection des Plantes 1, rue Le Nôtre, 75016 Paris, France 155 156 Diagnostic protocols Approval • laboratory procedures presented in the protocols may be adjusted to the standards of individual laboratories, provided EPPO Standards are approved by EPPO Council. The date of that they are adequately validated or that proper positive and approval appears in each individual standard. In the terms of negative controls are included. Article II of the IPPC, EPPO Standards are Regional Standards for the members of EPPO. References Review EPPO/CABI (1996) Quarantine Pests for Europe, 2nd edn. CAB Interna- tional, Wallingford (GB). EPPO Standards are subject to periodic review and amendment. EU (2000) Council Directive 2000/29/EC of 8 May 2000 on protective The next review date for this EPPO Standard is decided by the measures against the introduction into the Community of organisms EPPO Working Party on Phytosanitary Regulations harmful to plants or plant products and against their spread within the Community. Official Journal of the European Communities L169, 1–112. FAO (1997) International Plant Protection Convention (new revised text). Amendment record FAO, Rome (IT). IPPC (1993) Principles of plant quarantine as related to international trade. Amendments will be issued as necessary, numbered and dated. ISPM no. 1. IPPC Secretariat, FAO, Rome (IT). -
1 Method for the Detection of Pospiviroids on Tomato Seed Crop
Method for the Detection of Pospiviroids on Tomato Seed Crop Tomato (Solanum lycopersicum) Pathogens Pospiviroidae: Citrus exocortis viroid (CEVd), Columnea latent viroid (CLVd), Mexican papita viroid (MPVd), Pepper chat fruit viroid (PCFVd), Potato spindle tuber viroid (PSTVd), Tomato apical stunt viroid (TASVd), Tomato chlorotic dwarf viroid (TCDVd), Tomato planta macho viroid (TPMVd) ISHI-Veg recommends using the Naktuinbouw protocol Sample and sub-sample sizes The Naktuinbouw protocol uses 3,000 or 20,000 seeds in the sample. A sample size of 3,000 seeds with a maximum sub-sample size of 1,000 seeds is adequate for detecting pospiviroidae in tomato seed. NOTE: An important factor that determines the sample size of the seed to be tested is the epidemiology of the disease, i.e. 1. the rate of disease transmission from infected seed to seedling and 2. the spread of the disease in a seed production unit 1. Rate of disease transmission Several studies have experimentally demonstrated variable rates of transmission of pospiviroids from infected seed to seedlings (Kryczynski et al., 1988 for PSTVd, Singh and Dilworth, 2009 for TCDVd, Antignus et al., 2007 for TASVd). However, there are also studies in which no seed transmission was observed (Singh et al., 1999 and Matsushita et al., 2011 for TCDVd). In all these studies the infected seeds were obtained by artificially infecting mother plants. In the few studies using naturally infected seed lots, transmission rates are very low; 0,08 % for TCDVd based on 2500 seeds (Candresse et al., 2010) and 0,27% for PSTVd based on 370 seeds (Brunschot et al., 2014). -
Citrus Industry Biosecurity Plan 2015
Industry Biosecurity Plan for the Citrus Industry Version 3.0 July 2015 PLANT HEALTH AUSTRALIA | Citrus Industry Biosecurity Plan 2015 Location: Level 1 1 Phipps Close DEAKIN ACT 2600 Phone: +61 2 6215 7700 Fax: +61 2 6260 4321 E-mail: [email protected] Visit our web site: www.planthealthaustralia.com.au An electronic copy of this plan is available through the email address listed above. © Plant Health Australia Limited 2004 Copyright in this publication is owned by Plant Health Australia Limited, except when content has been provided by other contributors, in which case copyright may be owned by another person. With the exception of any material protected by a trade mark, this publication is licensed under a Creative Commons Attribution-No Derivs 3.0 Australia licence. Any use of this publication, other than as authorised under this licence or copyright law, is prohibited. http://creativecommons.org/licenses/by-nd/3.0/ - This details the relevant licence conditions, including the full legal code. This licence allows for redistribution, commercial and non-commercial, as long as it is passed along unchanged and in whole, with credit to Plant Health Australia (as below). In referencing this document, the preferred citation is: Plant Health Australia Ltd (2004) Industry Biosecurity Plan for the Citrus Industry (Version 3.0 – July 2015). Plant Health Australia, Canberra, ACT. Disclaimer: The material contained in this publication is produced for general information only. It is not intended as professional advice on any particular matter. No person should act or fail to act on the basis of any material contained in this publication without first obtaining specific and independent professional advice. -
EPPO Reporting Service
ORGANISATION EUROPEENNE ET MEDITERRANEENNE POUR LA PROTECTION DES PLANTES EUROPEAN AND MEDITERRANEAN PLANT PROTECTION ORGANIZATION EPPO Reporting Service NO. 10 PARIS, 2020-10 General 2020/209 New additions to the EPPO A1 and A2 Lists 2020/210 New data on quarantine pests and pests of the EPPO Alert List 2020/211 New and revised dynamic EPPO datasheets are available in the EPPO Global Database 2020/212 Recommendations from Euphresco projects Pests 2020/213 First report of Spodoptera frugiperda in Jordan 2020/214 Trogoderma granarium does not occur in Spain 2020/215 First report of Scirtothrips dorsalis in Mexico 2020/216 First report of Scirtothrips dorsalis in Brazil 2020/217 Scirtothrips dorsalis occurs in Colombia 2020/218 Update on the situation of Megaplatypus mutatus in Italy 2020/219 Update on the situation of Anoplophora chinensis in Croatia 2020/220 Update on the situation of Anoplophora chinensis in Italy 2020/221 Update on the situation of Anoplophora glabripennis in Italy Diseases 2020/222 Eradication of thousand canker disease in disease in Toscana (Italy) 2020/223 First report of tomato brown rugose fruit virus in the Czech Republic 2020/224 Update on the situation of tomato brown rugose fruit virus in Greece 2020/225 Update on the situation of tomato brown rugose fruit virus in the Netherlands 2020/226 New finding of ‘Candidatus Liberibacter solanacearum’ in Estonia 2020/227 Haplotypes and vectors of ‘Candidatus Liberibacter solanacearum’ in Scotland (United Kingdom) 2020/228 First report of wheat blast in Zambia and in -
Pospiviroidae Viroids in Naturally Infected Stone and Pome Fruits In
21st International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops Pospiviroidae viroids in naturally infected stone and pome fruits in Greece Kaponi, M.S.1, Luigi, M.2, Barba, M.2, Kyriakopoulou, P.E.I I Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece 2 CRA-PAV, Centro di Ricerca per la Patologia Vegeta le, 00156 Rome, Italy Abstract Viroid research on pome and stone fruit trees in Greece is important, as it seems that such viroids are widespread in the country and may cause serious diseases. Our research dealt with three Pospiviroidae species infecting pome and stone fruit trees, namely Apple scar skin viroid (ASSVd), Pear blister canker viroid (PBCVd) and Hop stunt viroid (HSVd). Tissue-print hybridization, reverse transcription-polymerase chain reaction (RT-PCR), cloning and sequencing techniques were successfully used for the detection and identification of these viroids in a large number of pome and stone fruit tree samples from various areas of Greece (Peloponnesus, Macedonia, Thessaly, Attica and Crete). The 58 complete viroid sequences obtained (30 ASSVd, 16 PBCVd and 12 HSVd) were submitted to the Gen Bank. Our results showed the presence of ASSVd in apple, pear, wild apple (Malus sylvestris), wild pear (Pyrus amygdaliformis) and sweet cherry; HSVd in apricot, peach, plum, sweet cherry, bullace plum (Prunus insititia), apple and wild apple; and PBCVd in pear, wild pear, quince, apple and wild apple. This research confirmed previous findings of infection of Hellenic apple, pear and wild pear with ASSVd, pear, wild pear and quince with PBCVd and apricot with HSVd.