Genetics: Published Articles Ahead of Print, published on December 1, 2005 as 10.1534/genetics.105.045542 An essential gene for fruiting body initiation in the basidiomycete Coprinopsis cinerea is homologous to bacterial cyclopropane fatty acid synthase genes Yi Liu*,1, Prayook Srivilai†, Sabine Loos*,2, Markus Aebi*, Ursula Kües† * Institute for Microbiology, ETH Zurich, CH-8093 Zurich, Switzerland † Molecular Wood Biotechnology, Institute for Forest Botany, Georg-August University Göttingen, D-37077 Göttingen, Germany 1 Present address: Laboratorio di Differenziamento Cellulare, Centro di Biotecnologie Avanzate, Largo R. Benzi 10, 16132 Genova, Italy 2 Present address: Hans Knöll Institut Jena e.V., Junior Research Group Bioorganic Synthesis, Beutenbergstr. 11a, D-07745 Jena, Germany Sequence data from this article have been deposited with the EMBL/Genbank Data Libraries under accession nos. AF338438 1 Running title: An essential fruiting initiation gene Key words: hyphal knots, primordia, sexual development, ∆24-sterol-C-methyltransferase, lipid modification Corresponding author: U. Kües, Georg-August University Göttingen, Institute for Forest Botany, Section Molecular Wood Biotechnology, Büsgenweg 2, D-37077 Göttingen, Germany Tel: ++49-551-397024 Fax: ++49-551-392705 e-mail:
[email protected] 2 ABSTRACT The self-compatible Coprinopsis cinerea homokaryon AmutBmut produces fruiting bodies without prior mating to another strain. Early stages of fruiting body development include the dark- dependent formation of primary hyphal knots and their light-induced transition to the more compact secondary hyphal knots. The AmutBmut UV mutant 6-031 forms primary hyphal knots, but development arrests at the transition state by a recessive defect in the cfs1 gene, isolated from a cosmid library by mutant complementation.