Biodiversity of Demersal Fish Along the Estuarine - Shelf Regions of Goa
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Biodiversity of demersal fish along the estuarine - shelf regions of Goa Thesis submitted to Goa University for the degree of Doctor of Philosophy in Marine Sciences 53 7 By 7 7 D I Vinay P. Padate Department of Marine Sciences Goa University, Goa - 403 206, INDIA October, 2010 5'2G Dedicated to Late Shri (Dr) Kethavrao Yoshi Statement As required by the University ordinance 0.19.8 (vi), I state that the present thesis entitled "Biodiversity of demersal fish along the estuarine — shelf regions of Goa" is my original contribution and the same has not been submitted on any previous occasion. To the best of my knowledge the present study is the first comprehensive work of its kind from the area mentioned. The literature related to the problem investigated has been cited. Due acknowledgements have been made wherever facilities and suggestions have been availed of. Vinay P. Padate Certificate This is to certify that the thesis entitled "Biodiversity of demersal fish along the estuarine — shelf regions of Goa", submitted by Mr. Vinay P. Padate for the award of Doctor of Philosophy in Marine Sciences is based on his original studies carried out by him under my supervision. The thesis or any part thereof has not been previously submitted for any degree or diploma in any Universities or • Institutions. Dr. C.U. • ivonker Research Guide Associate Professor Department of Marine Sciences Goa University Taleigao Plateau, 403 206, Goa Ai I t•K& carr'eafrow) sw-jog*__ce .4-e-r6'e4 eczAAd)rbitP3 ACKNOWLEDGEMENTS I would like to express a deep sense of gratitude to my guide, Dr. C.U. Rivonker, for giving me an opportunity to work on a unique research problem under his able guidance. I would like to express my gratitude to the Head, Department of Marine Sciences and members of the FRC for their timely suggestions. Further, thanks are due to the Director, National Institute of Oceanography, Goa for allowing me to participate in the Ballast water Project which has been funded by the Directorate General of Shipping, Government of India. I am highly indebted to Dr. A.C. Anil, Senior Scientist, MCMRD, NIO and coordinator of the project for the timely discussions that enhanced my scientific aptitude. I would always be indebted to Dr. S.S. Sawant, Senior Scientist, NIO for his help and support. I convey my gratitude to Mr. K. Venkat, Senior Technical Officer, NIO for extending technical assistance, Dr. Dattesh Desai, Scientist, NIO and Dr. Lidita Khandeparker, Scientist, NIO for their help in the DNA sequencing. I am also indebted to Mr V. Khedekar, Senior Technical Officer, GOD, NIO for the Scanning Electron Microscope (SEM) photography of the crab specimens. The help extended by Dr. M.P. Tapaswi, Documentation Officer, NIO in obtaining the references from different places is gratefully acknowledged. I also take this opportunity to express my deep gratitude to Dr. B.F. Chhapgar, an eminent carcinologist for his valuable suggestions in confirming the identity of the newly described species. Further, I acknowledge Dr. Mark Siddall, Curator at the American Museum of Natural History, New York for his timely help in providing Charybdis philippinensis specimens on loan for reference purpose. I thank the anonymous reviewers for the constructive criticism that enhanced the quality of the published papers. I would whole — heartedly thank my colleagues Nutan and Tomchou for teaching me the intricacies of data handling, processing and presentation, Vineel for the artwork techniques, Mahabaleshwar and Ganesh for their help extended during my stay at the Department. A special thanks to my colleagues at the MCMRD, NIO (Dhiraj, Rajat, Amar, Kirti, Lalita and Vinayak) for extending help during my work at the NIO. Finally, I take this opportunity to thank my close friends at the University, Shivraj, Santosh, Sweety, Shilpa, Renosh and Milind for supporting me during the study period. I would also like to thank the non — teaching staff of the Department of Marine Sciences for extending me all the help during my laboratory work. I wish to specially thank the fishing crew of the "Jesus Bless" trawler for allowing me to collect fish samples. I wish to express deep sense of gratitude to Shri. Dada Samant, Smt. Suvarna Teli, Shri. Prashant Padate and Shri. Satish Yedve for their blessings and support for my endeavour. I would also take this opportunity to specially thank Dr. Subodh Sawant for his kind support and encouragement. I make a special mention of my mother Late Mrs. Vandana Padate, whose memory has been the sole source of inspiration on my path to seek knowledge and my parents, Mr. Pandharinath and Mrs. Yamini Padate for their blessings, without which this endeavour would not have materialized. Vinay Padate CONTENTS Page No. List of Tables i — ii List of Figures iii — vi Chapter 1. General Introduction 1 1.1. Background information 1 1.2. Literature review 2 1.3. Objectives 6 Chapter 2. Materials and Methods 7 2.1. Study area 7 2.2. Sample collection 9 2.3. Taxonomic identification and morphometry 11 2.4. Sample preservation 12 2.5. 18S rDNA sequencing 12 2.6. Data compilation and processing 13 2.6.1. Faunal composition 13 2.6.2. Faunal abundance (numbers) and weight 13 2.6.3. Species diversity indices 14 2.6.3a. Shannon — Wiener's diversity index 14 2.6.3b. Heip's evenness index 15 2.6.3c. Margalefs species richness 15 2.6.3d. Simpson's dominance index 15 2.6.4. Cluster analysis 16 2.6.5. Functional guilds 16 2.6.6. Determination of catch rate 17 Chapter 3. Demersal faunal community structure off Goa 18 3.1. Introduction 18 3.2. Literature review 18 3.3. Results 22 3.3.1. Species composition 22 3.3.2. New records for the study area 23 3.3.3. Quantitative analysis 23 3.3.3 a. Overall data 23 3.3.3b. Crustaceans 26 3.3.3c. Fin fish 29 3.3.3d. Molluscs 36 3.3.3e. Other fauna 38 3.3.3f. Coastal residents and migrants 39 3.3.3g. Diversity 39 3.3.4. Faunal associations 41 3.3.4a. Family level 41 3.3.4b. Family level: pre — monsoon season 41 3.3.4c. Family level: post — monsoon season 42 3.3.4d. Species level 43 3.3.4e. Species level: pre — monsoon season 43 3.3.4f. Species level: post — monsoon season 44 3.3.5. Catch rates of the study area 45 3.4. Discussion 46 3.5. Conclusion 61 Chapter 4. Description of Charybdis (Charybdis) goaensis, new species 62 4.1. Introduction 62 4.2. Literature review 62 4.3. Taxonomy 65 4.3.1. Family Portunidae Rafinesque — Schmaltz, 1815 65 4.3.2. Subfamily Thalamitinae Paul'son, 1875 66 4.3.3. Genus Charybdis De Haan, 1833 66 4.3.4. Charybdis (Charybdis) goaensis sp. nov. 67 4.3.4a. Material examined 67 4.3.4b. Diagnosis 67 4.3.4c. Description 68 4.3.4d. Colour 71 4.3.4e. Etymology 71 4.3.4f. Distribution 71 4.3.4g. Habitat characteristics 71 4.3.4h. Habit 72 4.3.4i. Remarks 72 4.3.5. Charybdis (Charybdis) philippinensis Ward, 1941 74 4.3.5a. Original description by Ward (1941) 74 4.3.5b. Updated description of type specimens — C. philippinensis 76 4.3.6. Key to the species of swimming crabs of the sub — genus Charybdis 78 4.4. Conclusion 87 Chapter 5. A new record of Caesio cuning outside its known geographical array 88 5.1. Introduction 88 5.2. Literature review 88 5.3. Taxonomy 89 5.3.1. Family Caesionidae 89 5.3.2. Genus Caesio Lacepede, 1801 (Carpenter, 1988) 90 5.3.3. Caesio cuning (Bloch, 1791) . 90 5.3.3a. Material examined 90 5.3.3b. Description 91 5.4. Discussion 92 5.5. Conclusion 96 Chapter 6. A new record of Scylla olivacea from Goa — A comparative diagnosis 97 6.1. Introduction 97 6.2. Literature review 97 6.3. Taxonomy 102 6.3.1. Morph 1 103 6.3.1a. Material examined 103 6.3.1b. Description 103 6.3.2. Scylla olivacea (Herbst, 1796) description by Keenan et al. (1998) 105 6.3.3. Morph 2 105 6.3.3a. Material examined 105 6.3.3b. Description 106 6.3.4. Statistical analysis 108 6.3.5. DNA sequencing 108 6.3.6. Geographical distribution of Scylla olivacea 108 6.4. Conclusion 109 Chapter 7. Puffer fish diversity off Goa 110 7.1. Introduction 110 7.2. Literature review 110 7.3. Taxonomy 111 7.3.1. Family Tetraodontidae Fritzsche, 1982 111 7.3.2. Arothron immaculatus (Bloch & Schneider, 1801) 114 7.3.3. Chelonodon patoca (Hamilton, 1822) 115 7.3.4. Lagocephalus spadiceus (Richardson, 1845) 116 7.3.5. Takifugu oblongus (Bloch, 1786) 117 7.3.6. Genus Tetraodon Linnaeus, 1758 118 7.3.7. Tetraodon fluviatilis fluviatilis Hamilton, 1822 121 7.3.7a. Dekkers' (1975) description 121 7.3.7b. Present specimens — comparison with Dekkers' description 122 7.3.7c. DNA sequencing 123 7.4. Discussion 123 7.5. Conclusion 124 Chapter 8. Summary 125 Bibliography 127 Appendix List of Tables Table 2.1. Details of trawl sampling carried out along the potential fishing grounds and bay — estuarine waters of Goa Table 2.2. Details of shore sampling (beach seine and crab trap) carried out along the bay — estuarine waters of Goa Table 2.3. List of meristic counts used in the identification of demersal marine fauna Table 2.4. List of morphometric parameters used in the identification of demersal marine fauna Table 2.5.