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Toxic Effects of Crude Venom of a Desert Cobra, Walterinnesia Aegyptia, on Liver, Abdominal Muscles and Brain of Male Albino Rats
Pakistan J. Zool., vol. 45(5), pp. 1359-1366, 2013 Toxic Effects of Crude Venom of a Desert Cobra, Walterinnesia aegyptia, on Liver, Abdominal Muscles and Brain of Male Albino Rats Mohammed Khalid Al-Sadoon,1 * Gamal Mohamed Orabi1,2 and Gamal Badr3,4 1Zoology Department, College of Science, King Saud University, P.O. Box 2455Riyadh 11451, Saudi Arabia 2Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt 3Princes Johara Alibrahim Center for Cancer Research, Prostate Cancer Research Chair, College of Medicine, King Saud University, Riyadh, Saudi Arabia 4Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt Abstract.- The toxic effect of an acute dose of Walterinnesia aegyptia crude venom was studied in male albino rats. Liver enzymes,alaninetransaminase (ALT), aspartate transaminase (AST) and gamma glutamyltransferase(γ-GT), total protein concentration and Alkaline phosphatase(ALP) enzyme activity in the liver, abdominal muscles and cerebrum brain were measured at timed intervals of 1, 3, 6, 12, 24, 72 h and 7 days post envenomation. The histological changes in the liver sections were simultaneously investigated. These parameters were found to be fluctuated with time, with a tendency to regain to normal control levels within the first 6 h. Histological changes induced by treatment with LD50 of W. aegyptia crude venom in liver 3 to 6 hours post envenomation showed inflammatory cellular infiltrations(ICI) around the hepatic vein, dilated blood sinusoids (S), hepatocyticvacuolations (HV) and prominent van kuffer cells. The 12 to 24 h period seems to be crucial for the process of physiological recovery. Histological changes induced by treatment with LD50 of W. -
Medically Important Differences in Snake Venom Composition Are Dictated by Distinct Postgenomic Mechanisms
Medically important differences in snake venom composition are dictated by distinct postgenomic mechanisms Nicholas R. Casewella,b,1, Simon C. Wagstaffc, Wolfgang Wüsterb, Darren A. N. Cooka, Fiona M. S. Boltona, Sarah I. Kinga, Davinia Plad, Libia Sanzd, Juan J. Calveted, and Robert A. Harrisona aAlistair Reid Venom Research Unit and cBioinformatics Unit, Liverpool School of Tropical Medicine, Liverpool L3 5QA, United Kingdom; bMolecular Ecology and Evolution Group, School of Biological Sciences, Bangor University, Bangor LL57 2UW, United Kingdom; and dInstituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Científicas, 11 46010 Valencia, Spain Edited by David B. Wake, University of California, Berkeley, CA, and approved May 14, 2014 (received for review March 27, 2014) Variation in venom composition is a ubiquitous phenomenon in few (approximately 5–10) multilocus gene families, with each snakes and occurs both interspecifically and intraspecifically. family capable of producing related isoforms generated by Venom variation can have severe outcomes for snakebite victims gene duplication events occurring over evolutionary time (1, 14, by rendering the specific antibodies found in antivenoms in- 15). The birth and death model of gene evolution (16) is fre- effective against heterologous toxins found in different venoms. quently invoked as the mechanism giving rise to venom gene The rapid evolutionary expansion of different toxin-encoding paralogs, with evidence that natural selection acting on surface gene families in different snake lineages is widely perceived as the exposed residues of the resulting gene duplicates facilitates main cause of venom variation. However, this view is simplistic subfunctionalization/neofunctionalization of the encoded proteins and disregards the understudied influence that processes acting (15, 17–19). -
Cretaceous Fossil Gecko Hand Reveals a Strikingly Modern Scansorial Morphology: Qualitative and Biometric Analysis of an Amber-Preserved Lizard Hand
Cretaceous Research 84 (2018) 120e133 Contents lists available at ScienceDirect Cretaceous Research journal homepage: www.elsevier.com/locate/CretRes Cretaceous fossil gecko hand reveals a strikingly modern scansorial morphology: Qualitative and biometric analysis of an amber-preserved lizard hand * Gabriela Fontanarrosa a, Juan D. Daza b, Virginia Abdala a, c, a Instituto de Biodiversidad Neotropical, CONICET, Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucuman, Argentina b Department of Biological Sciences, Sam Houston State University, 1900 Avenue I, Lee Drain Building Suite 300, Huntsville, TX 77341, USA c Catedra de Biología General, Facultad de Ciencias Naturales, Universidad Nacional de Tucuman, Argentina article info abstract Article history: Gekkota (geckos and pygopodids) is a clade thought to have originated in the Early Cretaceous and that Received 16 May 2017 today exhibits one of the most remarkable scansorial capabilities among lizards. Little information is Received in revised form available regarding the origin of scansoriality, which subsequently became widespread and diverse in 15 September 2017 terms of ecomorphology in this clade. An undescribed amber fossil (MCZ Re190835) from mid- Accepted in revised form 2 November 2017 Cretaceous outcrops of the north of Myanmar dated at 99 Ma, previously assigned to stem Gekkota, Available online 14 November 2017 preserves carpal, metacarpal and phalangeal bones, as well as supplementary climbing structures, such as adhesive pads and paraphalangeal elements. This fossil documents the presence of highly specialized Keywords: Squamata paleobiology adaptive structures. Here, we analyze in detail the manus of the putative stem Gekkota. We use Paraphalanges morphological comparisons in the context of extant squamates, to produce a detailed descriptive analysis Hand evolution and a linear discriminant analysis (LDA) based on 32 skeletal variables of the manus. -
Cobra Risk Assessment
Invasive animal risk assessment Biosecurity Queensland Agriculture Fisheries and Department of Cobra (all species) Steve Csurhes and Paul Fisher First published 2010 Updated 2016 Pest animal risk assessment © State of Queensland, 2016. The Queensland Government supports and encourages the dissemination and exchange of its information. The copyright in this publication is licensed under a Creative Commons Attribution 3.0 Australia (CC BY) licence. You must keep intact the copyright notice and attribute the State of Queensland as the source of the publication. Note: Some content in this publication may have different licence terms as indicated. For more information on this licence visit http://creativecommons.org/licenses/ by/3.0/au/deed.en" http://creativecommons.org/licenses/by/3.0/au/deed.en Photo: Image from Wikimedia Commons (this image is reproduced under the terms of a GNU Free Documentation License) Invasive animal risk assessment: Cobra 2 Contents Summary 4 Introduction 5 Identity and taxonomy 5 Taxonomy 3 Description 5 Diet 5 Reproduction 6 Predators and diseases 6 Origin and distribution 7 Status in Australia and Queensland 8 Preferred habitat 9 History as a pest elsewhere 9 Uses 9 Pest potential in Queensland 10 Climate match 10 Habitat suitability 10 Broad natural geographic range 11 Generalist diet 11 Venom production 11 Disease 11 Numerical risk analysis 11 References 12 Attachment 1 13 Invasive animal risk assessment: Cobra 3 Summary The common name ‘cobra’ applies to 30 species in 7 genera within the family Elapidae, all of which can produce a hood when threatened. All cobra species are venomous. As a group, cobras have an extensive distribution over large parts of Africa, Asia, Malaysia and Indonesia. -
Original Papers Helminth Parasites of the White-Spotted Wall Gecko
Annals of Parasitology 2019, 65(1), 71-75 Copyright© 2019 Polish Parasitological Society doi: 10.17420/ap6501.184 Original papers Helminth parasites of the white-spotted wall gecko, Tarentola annularis (Squamata: Gekkonidae), from Shendi area, Sudan Yassir Sulieman 1, Randa E. Eltayeb 1, Natchadaporn Srimek 2, Theerakamol Pengsakul 3 1Department of Zoology, Faculty of Science and Technology, University of Shendi, Shendi, Sudan 2Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla, Thailand 3Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla, Thailand Corresponding Author: Yassir Sulieman; e-mail: [email protected] ABSTRACT. This is the first report on helminths parasitize the white-spotted wall gecko, Tarentola annularis from Shendi area in Sudan. A total of 32 geckos were collected between January and May 2018, and examined for helminth infections. Three nematode species of the family Pharyngodonidae were identified: Pharyngodon mamillatus , Spauligodon brevibursata and Parapharyngodon sp. The most prevalent nematode found was P. mamillatus followed by S. brevibursata . The overall prevalence and intensity of infections was 81.3% and 6.8 nematodes per one infected gecko, respectively. The prevalence and intensity of infections were observed to be more in adult male geckos compared to adult females. On the other hand, the prevalence and intensity of infections were significantly higher in adult geckos compared to the juveniles. Key words: Tarentola annularis , helminth, prevalence, intensity, Sudan Introduction small vertebrates [6,8]. Previous reports reveal infection of T. annularis with different parasite Different genera of geckos are known as species [9 −13]. However, in general, gecko species common reptile of human dwellings around the are reported to be parasitized by various parasite world. -
Experience of Snakebite Envenomation by a Desert Viper in Qatar
Hindawi Journal of Toxicology Volume 2020, Article ID 8810741, 5 pages https://doi.org/10.1155/2020/8810741 Review Article Experience of Snakebite Envenomation by a Desert Viper in Qatar Amr Elmoheen ,1 Waleed Awad Salem ,1 Mahmoud Haddad ,1 Khalid Bashir ,1 and Stephen H. Thomas1,2,3 1Department of Emergency Medicine, Hamad Medical Corporation, Doha, Qatar 2Weill Cornell Medical College in Qatar, Doha, Qatar 3Barts and "e London School of Medicine, Queen Mary University of London, London, UK Correspondence should be addressed to Amr Elmoheen; [email protected] Received 8 June 2020; Revised 8 September 2020; Accepted 28 September 2020; Published 12 October 2020 Academic Editor: Mohamed M. Abdel-Daim Copyright © 2020 Amr Elmoheen et al. &is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Crotaline and elapid snakebites are reported all over the world as well as in the Middle East and other countries around this region. However, data regarding snakebites and their treatment in Qatar are limited. &is review paper is going to investigate the presentation and treatment of snakebite in Qatar. A good assessment helps to decide on the management of the snakebites envenomation. Antivenom and conservative management are the mainstays of treatment for crotaline snakebite. Point-of-care ultrasound (POCUS) has been suggested to do early diagnosis and treatment of soft tissue problems, such as edema and compartment syndrome, after a snakebite. &e supporting data are not sufficient regarding the efficiency of POCUS in diagnosing the extent and severity of tissue involvement and its ultimate effect on the outcome. -
Ancestrally High Elastic Modulus of Gecko Setal B-Keratin
J. R. Soc. Interface (2007) 4, 1071–1076 doi:10.1098/rsif.2007.0226 Published online 20 March 2007 Ancestrally high elastic modulus of gecko setal b-keratin Anne M. Peattie1,*, Carmel Majidi2, Andrew Corder1,† and Robert J. Full1 1Department of Integrative Biology, University of California, Berkeley, 3060 Valley Life Sciences Building, Berkeley, CA 94720-3140, USA 2Department of Electrical Engineering and Computer Science, University of California, Berkeley, 333 Cory Hall, Berkeley, CA 94720-1770, USA Typical bulk adhesives are characterized by soft, tacky materials with elastic moduli well below 1 MPa. Geckos possess subdigital adhesives composed mostly of b-keratin, a relatively stiff material. Biological adhesives like those of geckos have inspired empirical and modelling research which predicts that even stiff materials can be effective adhesives if they take on a fibrillar form. The molecular structure of b-keratin is highly conserved across birds and reptiles, suggesting that material properties of gecko setae should be similar to that of b-keratin previously measured in birds, but this has yet to be established. We used a resonance technique to measure elastic bending modulus in two species of gecko from disparate habitats. We found no significant difference in elastic modulus between Gekko gecko (1.6 GPaG0.15 s.e.; nZ24 setae) and Ptyodactylus hasselquistii (1.4 GPaG0.15 s.e.; nZ24 setae). If the elastic modulus of setal keratin is conserved across species, it would suggest a design constraint that must be compensated for structurally, and possibly explain the remarkable variation in gecko adhesive morphology. Keywords: gecko; seta; fibrillar adhesive; keratin; b-keratin; elastic modulus 1. -
(Cerastes) VENOM
Received: June 20, 2005 J. Venom. Anim. Toxins incl. Trop. Dis. Accepted: October 27, 2005 V.12, n.3, p.400-417, 2006. Abstract published online: December 14, 2005 Original paper. Full paper published online: August 31, 2006 ISSN 1678-9199. PHARMACOLOGICAL CHARACTERIZATION OF RAT PAW EDEMA INDUCED BY Cerastes gasperettii (cerastes) VENOM AL-ASMARI A. K. (1), ABDO N. M. (1) (1) Research Center, Armed Forces Hospital, Riyadh, Saudi Arabia. ABSTRACT: Inflammatory response induced by the venom of the Arabian sand viper Cerastes gasperettii was studied by measuring rat hind-paw edema. Cerastes gasperettii venom (CgV, 3.75-240 µg/paw), heated for 30s at 97°C, caused a marked dose and time-dependent edema in rat paw. Response was maximal 2h after venom administration and ceased within 24h. Heated CgV was routinely used in our experiments at the dose of 120 µg/paw. Among all the drugs and antivenoms tested, cyproheptadine and 5-nitroindazole were the most effective in inhibiting edema formation. Aprotinin, mepyramine, dexamethasone, diclofenac, dipyridamole, Nω- nitro-L-arginine, quinacrine, and nordihydroguaiaretic acid showed statistically (p<0.001) significant inhibitory effect, but with variations in their inhibition degree. Equine polyspecific and rabbit monospecific antivenoms significantly (p<0.001) reduced edema when locally administered (subplantar) but were ineffective when intravenously injected. We can conclude that the principal inflammatory mediators were serotonin, histamine, adenosine transport factors, phosphodiesterase (PDE), cyclooxygenase, lipoxygenase and phospholipase A2 (PLA2), in addition to other prostaglandins and cytokines. KEY WORDS: inflammatory mediators, Cerastes gasperettii venom, edema, antagonist, antivenom. CORRESPONDENCE TO: ABDULRAHMAN KHAZIM AL-ASMARI. P.O. -
XIV Jornades Herpetològiques Catalanes
XIV Jornades Herpetològiques Catalanes Marçà (Priorat) 10 i 11 de novembre de 2012 ORGANITZADES PER: AMB LA COL·LABORACIÓ DE: SOCIETAT CATALANA D’HERPETOLOGIA XIV JORADES HERPETOLÒGIQUES CATALAES Llibre de Resums Marçà (Priorat, Catalunya) 10 i 11 d’octubre de 2012 Organitzades per: Socetat Catalana d’Herpetologia Amb la col·laboració de: Grup d’Estudi i Protecció dels Ecosistemes Catalans – Ecologistes de Catalunya Ajuntament de Marçà Institució Catalana d’Història atural 2 PRESETACIÓ DE LES XIV JORADES HERPETOLÒGIQUES CATALAES Marçà (Priorat, Catalunya) 10 i 11 d’octubre de 2012 Des de la Societat Catalana d’Herpetologia (SCH), i també des de les enti- tats col·laboradores Grup d’Estudi i Protecció dels Ecosistemes Catalans – Ecologistes de Catalunya (GEPEC.EdC), Ajuntament de Marçà i la Institu- ció Catalana d’Història Natural (ICHN), es fa un esforç important perquè les Jornades Herpetològiques de Marçà esdevinguin el punt de trobada tant d’herpetòlegs professionals com d’afeccionats als amfibis i rèptils. En aquest sentit, i com en les anteriors edicions, l’esperit de les Jornades dóna cabuda a estudiants i persones que s’inicien en aquest món, en emular una escola de joves naturalistes a l’estil d’antigues trobades que s’organitzaven, ja fa dècades, al nostre país. Amb la presència de científics d’alt nivell, i alhora pedagògics, amens i propers, amb presentacions de gran qualitat, esperem satisfer les expectatives de tothom, i que ens trobem tots, aquest cap de setmana a Marçà, on podrem preguntar, aprendre, compartir vivèn- cies, inquietuds, etc. Os desitgem que gaudiu d’aquestes Jornades SOCIETAT CATALAA D’HERPETOLOGIA SOCIETAT CATALANA D’HERPETOLOGIA Museu de Ciències Naturals de Barcelona. -
Actiflagelin, a New Sperm Activator Isolated From
Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening Tarek Mohamed Abd El-Aziz, Sawsan Al Khoury, Lucie Jaquillard, Mathilde Triquigneaux, Guillaume Martinez, Sandrine Bourgoin-Voillard, Michel Sève, Christophe Arnoult, Rémy Beroud, Michel de Waard To cite this version: Tarek Mohamed Abd El-Aziz, Sawsan Al Khoury, Lucie Jaquillard, Mathilde Triquigneaux, Guillaume Martinez, et al.. Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening. Journal of Venomous Animals and Toxins including Tropical Diseases, Biomed Central, 2018, 24 (1), pp.2. 10.1186/s40409-018-0140-4. hal-01833333 HAL Id: hal-01833333 https://hal.archives-ouvertes.fr/hal-01833333 Submitted on 11 Jul 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Abd El-Aziz et al. Journal of Venomous Animals and Toxins including Tropical Diseases (2018) 24:2 DOI 10.1186/s40409-018-0140-4 RESEARCH Open Access Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening Tarek Mohamed Abd El-Aziz1,2,3†, Sawsan Al Khoury1,2†, Lucie Jaquillard4, Mathilde Triquigneaux4, Guillaume Martinez5,6, Sandrine Bourgoin-Voillard5,7,8, Michel Sève5,7,8, Christophe Arnoult5,6, Rémy Beroud4 and Michel De Waard1,2,4* Abstract Background: Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. -
Classic Versus Metabarcoding Approaches for the Diet Study of a Remote Island Endemic Gecko
Questioning the proverb `more haste, less speed': classic versus metabarcoding approaches for the diet study of a remote island endemic gecko Vanessa Gil1,*, Catarina J. Pinho2,3,*, Carlos A.S. Aguiar1, Carolina Jardim4, Rui Rebelo1 and Raquel Vasconcelos2 1 Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal 2 CIBIO, Centro de Investigacão¸ em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Universidade do Porto, Vairão, Portugal 3 Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal 4 Instituto das Florestas e Conservacão¸ da Natureza IP-RAM, Funchal, Madeira, Portugal * These authors contributed equally to this work. ABSTRACT Dietary studies can reveal valuable information on how species exploit their habitats and are of particular importance for insular endemics conservation as these species present higher risk of extinction. Reptiles are often neglected in island systems, principally the ones inhabiting remote areas, therefore little is known on their ecological networks. The Selvagens gecko Tarentola (boettgeri) bischoffi, endemic to the remote and integral reserve of Selvagens Archipelago, is classified as Vulnerable by the Portuguese Red Data Book. Little is known about this gecko's ecology and dietary habits, but it is assumed to be exclusively insectivorous. The diet of the continental Tarentola species was already studied using classical methods. Only two studies have used next-generation sequencing (NGS) techniques for this genus thus far, and very few NGS studies have been employed for reptiles in general. Considering the lack of information on its diet Submitted 10 April 2019 and the conservation interest of the Selvagens gecko, we used morphological and Accepted 22 October 2019 Published 2 January 2020 DNA metabarcoding approaches to characterize its diet. -
Conservation of South African Tortoises with Emphasis on Their Apicomplexan Haematozoans, As Well As Biological and Metal-Fingerprinting of Captive Individuals
CONSERVATION OF SOUTH AFRICAN TORTOISES WITH EMPHASIS ON THEIR APICOMPLEXAN HAEMATOZOANS, AS WELL AS BIOLOGICAL AND METAL-FINGERPRINTING OF CAPTIVE INDIVIDUALS By Courtney Antonia Cook THESIS submitted in fulfilment of the requirements for the degree PHILOSOPHIAE DOCTOR (Ph.D.) in ZOOLOGY in the FACULTY OF SCIENCE at the UNIVERSITY OF JOHANNESBURG Supervisor: Prof. N. J. Smit Co-supervisors: Prof. A. J. Davies and Prof. V. Wepener June 2012 “We need another and a wiser and perhaps a more mystical concept of animals. Remote from universal nature, and living by complicated artifice, man in civilization surveys the creature through the glass of his knowledge and sees thereby a feather magnified and the whole image in distortion. We patronize them for their incompleteness, for their tragic fate of having taken form so far below ourselves. And therein we err, and greatly err. For the animal shall not be measured by man. In a world older and more complete than ours they move finished and complete, gifted with extensions of the senses we have lost or never attained, living by voices we shall never hear. They are not brethren, they are not underlings; they are other nations caught with ourselves in the net of life and time, fellow prisoners of the splendour and travail of the earth.” Henry Beston (1928) ABSTRACT South Africa has the highest biodiversity of tortoises in the world with possibly an equivalent diversity of apicomplexan haematozoans, which to date have not been adequately researched. Prior to this study, five apicomplexans had been recorded infecting southern African tortoises, including two haemogregarines, Haemogregarina fitzsimonsi and Haemogregarina parvula, and three haemoproteids, Haemoproteus testudinalis, Haemoproteus balazuci and Haemoproteus sp.