CBSE Class 8 Science Chapter 8 Cell-Structure and Functions Objective Questions
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The Morphology, Ultrastructure and Molecular Phylogeny of a New Freshwater Heterolobose Amoeba Parafumarolamoeba Stagnalis N. Sp
diversity Article The Morphology, Ultrastructure and Molecular Phylogeny of a New Freshwater Heterolobose Amoeba Parafumarolamoeba stagnalis n. sp. (Vahlkampfiidae; Heterolobosea) Anastasia S. Borodina 1,2, Alexander P. Mylnikov 1,†, Jan Janouškovec 3 , Patrick J. Keeling 4 and Denis V. Tikhonenkov 1,5,* 1 Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, 152742 Borok, Russia; [email protected] 2 Department of Zoology and Parasitology, Voronezh State University, Universitetskaya Ploshad 1, 394036 Voronezh, Russia 3 Centre Algatech, Laboratory of Photosynthesis, Institute of Microbiology, Czech Academy of Sciences, Opatovický Mlýn, 37981 Tˇreboˇn,Czech Republic; [email protected] 4 Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T1Z4, Canada; [email protected] 5 AquaBioSafe Laboratory, University of Tyumen, 625003 Tyumen, Russia * Correspondence: [email protected]; Tel.: +7-485-472-4533 † Alexander P. Mylnikov is deceased. http://zoobank.org/References/e543a49a-16c1-4b7c-afdb-0bc56b632ef0 Abstract: Heterolobose amoebae are important members of marine, freshwater, and soil microbial Citation: Borodina, A.S.; Mylnikov, communities, but their diversity remains under-explored. We studied the diversity of Vahlkampfiidae A.P.; Janouškovec, J.; Keeling, P.J.; to improve our understanding of heterolobosean relationships and their representation in aquatic Tikhonenkov, D.V. The Morphology, benthos. Using light and electron microscopy, and molecular phylogenies based on the SSU rRNA Ultrastructure and Molecular and ITS loci, we describe the fine morphology and evolutionary relationships of a new heterolobosean Phylogeny of a New Freshwater Parafumarolamoeba stagnalis n. sp. from a small pond in European Russia. Cells of P. stagnalis possess Heterolobose Amoeba a clearly distinguishable anterior hyaline pseudopodium, eruptive movement, several thin and Parafumarolamoeba stagnalis n. -
Protein Import Into Isolated Pea Root Leucoplasts
ORIGINAL RESEARCH published: 04 September 2015 doi: 10.3389/fpls.2015.00690 Protein import into isolated pea root leucoplasts Chiung-Chih Chu and Hsou-min Li* Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan Leucoplasts are important organelles for the synthesis and storage of starch, lipids and proteins. However, molecular mechanism of protein import into leucoplasts and how it differs from that of import into chloroplasts remain unknown. We used pea seedlings for both chloroplast and leucoplast isolations to compare within the same species. We further optimized the isolation and import conditions to improve import efficiency and to permit a quantitative comparison between the two plastid types. The authenticity of the import was verified using a mitochondrial precursor protein. Our results show that, when normalized to Toc75, most translocon proteins are less abundant in leucoplasts than in chloroplasts. A precursor shown to prefer the receptor Toc132 indeed had relatively more similar import efficiencies between chloroplasts and leucoplasts compared to precursors that prefer Toc159. Furthermore we found two precursors that exhibited very high import efficiency into leucoplasts. Their transit peptides may be candidates Edited by: for delivering transgenic proteins into leucoplasts and for analyzing motifs important for Bo Liu, University of California, Davis, USA leucoplast import. Reviewed by: Keywords: leucoplasts, plastid, root, protein import, translocon Kentaro Inoue, University of California, Davis, USA Danny Schnell, Introduction University of Massachusetts, USA *Correspondence: Plastids are essential plant organelles responsible for functions ranging from photosynthesis and Hsou-min Li, biosynthesis of amino acids and fatty acids to assimilation of nitrogen and sulfur (Leister, 2003; Institute of Molecular Biology, Sakamoto et al., 2008). -
Can Protozoa Prove the Beginning of the World?
Southeastern University FireScholars Classical Conversations Spring 2020 Can Protozoa Prove the Beginning of the World? Karina L. Burton Southeastern University - Lakeland, [email protected] Follow this and additional works at: https://firescholars.seu.edu/ccplus Part of the Cell Biology Commons, and the Evolution Commons Recommended Citation Burton, Karina L., "Can Protozoa Prove the Beginning of the World?" (2020). Classical Conversations. 9. https://firescholars.seu.edu/ccplus/9 This Term Paper is brought to you for free and open access by FireScholars. It has been accepted for inclusion in Classical Conversations by an authorized administrator of FireScholars. For more information, please contact [email protected]. 1 Can Protozoa Prove the Beginning of the World? Karina L. Burton Classical Conversations: Challenge 4; Southeastern University ENGL 1233: English Composition II Grace Veach April 16, 2020 2 Abstract Protozoa are magnificent creatures. They exhibit all of the functions intrinsic to living organisms: irritability, metabolism, growth and reproduction. Within these functions, there are numerous examples of mutations that occur in order for organisms to adapt to their given environments. Irritability is demonstrated in protozoa by their use of pseudopodia, flagella, or cilia for motility; it has been shown that such locomotors exhibit diversity while maintaining similar protein and chemical structures that appear to be a result of evolutionary processes. Metabolism in protozoa is similar to that of larger animals, but their diet is unique. They primarily feast upon bacteria, which have begun mutating to evade easy ingestion and digestion by protozoa, therefore increasing their survival rate and making it necessary for protozoa to adapt. -
Amoeba, Paramoecium, Euglena and Diatom
BIOLOGY CLASSIFICATION OF PLANTS Kingdom Protista Introduction to Kingdom Protista Kingdom Protista consists of unicellular organisms. They contain a well-defined nucleus and a nucleolus enclosed in a nuclear membrane. The protoplasm is surrounded by the plasma membrane. The cytoplasm contains various cell organelles. Nuclear material is organised in the form of a linear, double-stranded and helical DNA, along with proteins. The mode of nutrition is either autotrophic or heterotrophic. Examples: Amoeba, Paramoecium, Euglena and diatom Amoeba Paramoecium Euglena Diatom Amoeba Amoeba is microscopic and one of the simplest organisms made of just one cell. It is found in ponds, ditches, mud or on submerged water plants in freshwater bodies. The body of Amoeba is irregular in shape. The outer membrane called cell membrane or plasmalemma encloses the cytoplasm. A prominent nucleus, several food vacuoles, a contractile vacuole and reserve food granules are present. Amoeba uses pseudopodia for feeding and locomotion. It reproduces by binary fission. During unfavourable conditions (e.g. drying up of pond), it forms a protective cyst within which it reproduces by multiple fission, producing several offspring. Structure of Amoeba www.topperlearning.com 2 BIOLOGY CLASSIFICATION OF PLANTS Movement in Amoeba Movement in Amoeba occurs with the help of temporary or false feet called pseudopodia. Pseudopodia are finger-like projections formed by the flowing of cytoplasm into these extensions. At a time, several pseudopodia can be seen projecting out from the body of an Amoeba. However, only one of them extends longer than the others towards the direction it wants to move. This type of movement is called amoeboid movement. -
Protozoa : Locomotion and Nutrition
Protozoa : locomotion and Nutrition Lecturer : P. V. Deokar Department of Zoology R.K.M.M. Ahmednagar Locomotion in Protozoa • The following points highlight the three main types of locomotion exhibited by protozoans. The types of locomotion are: • 1. Amoeboid Movement • 2. Flagellar Movement • 3. Ciliary Movement. Protozoans: Type of Locomotion # 1. Amoeboid Movement: • movement of the animal is made by the throwing of pseudopodium, called amoeboid movement • In the direction of movement of Amoeba a new pseudopodium is formed and the pseudopodium at the opposite side gradually disappears. • Types of pseudopodia: • According to form, structure and activity four different kinds of pseudopodia are recognised • These are: • (a) Lobopodium • (b) Filopodium • (c) Reticulopodium or Rhizopodium • (d) Axopodium or Actinopodium (a) Lobopodium [Gk. lobes = lobe; podium = foot]: • It is a short, finger or tongue-like projection which is accompanied by a flow of endoplasm and ectoplasm. • The pseudopodium is broad with rounded or blunt tips. • The ectoplasmmic area is distinctly clear, called the hyaline cap. • It is the characteristic of many amoebas such as Amoeba. (b) Filopodium [L.filo = a thread; podium = foot]: • The filopodium is a slender, thread-like or filamentous projection. • It is formed by the ectoplasm alone and without a hyaline cap. • The filaments are narrow and may be branched but do not anastomose, Filopodium is the characteristic in Filosea (e.g., Euglypha ). (c) Reticulopodium or Rhizopodium [L. reticulos = a net, podium = foot]: • Similar in structure to that of filopodium but the branches anastomose. • The numerous branched and anastomosed pseudopodia form a dense network, help primarily in capturing the prey and the secondary function is locomotion. -
Brown Algae and 4) the Oomycetes (Water Molds)
Protista Classification Excavata The kingdom Protista (in the five kingdom system) contains mostly unicellular eukaryotes. This taxonomic grouping is polyphyletic and based only Alveolates on cellular structure and life styles not on any molecular evidence. Using molecular biology and detailed comparison of cell structure, scientists are now beginning to see evolutionary SAR Stramenopila history in the protists. The ongoing changes in the protest phylogeny are rapidly changing with each new piece of evidence. The following classification suggests 4 “supergroups” within the Rhizaria original Protista kingdom and the taxonomy is still being worked out. This lab is looking at one current hypothesis shown on the right. Some of the organisms are grouped together because Archaeplastida of very strong support and others are controversial. It is important to focus on the characteristics of each clade which explains why they are grouped together. This lab will only look at the groups that Amoebozoans were once included in the Protista kingdom and the other groups (higher plants, fungi, and animals) will be Unikonta examined in future labs. Opisthokonts Protista Classification Excavata Starting with the four “Supergroups”, we will divide the rest into different levels called clades. A Clade is defined as a group of Alveolates biological taxa (as species) that includes all descendants of one common ancestor. Too simplify this process, we have included a cladogram we will be using throughout the SAR Stramenopila course. We will divide or expand parts of the cladogram to emphasize evolutionary relationships. For the protists, we will divide Rhizaria the supergroups into smaller clades assigning them artificial numbers (clade1, clade2, clade3) to establish a grouping at a specific level. -
Microscope Studies on the Morphogenesis of Plastids V
Bot. Mag. Tokyo 84: 123-136 (March 25, 1971) Electron Microscope Studies on the Morphogenesis of Plastids V. Concerning One-dimensional Metamorphosis of the Plastids in Cryptomeriac Leaves* by Susumu TOYAMA** and Kazuo FUNAZAKIS** Received November 25, 1970 Abstract To know about the mechanism of interconversion of plastids, electron micro- scopic observations were made on Cryptomeria leaves which aquire a reddish brown color in winter and recover their green color in coming spring to summer. In normal green leaves, two different kinds of plastids have been observed, viz, the chloroplasts having well organized grana structure in mesophyll cells and those completely lacking grana lamellae in bundle sheath cells. General feature of plastids in reddish brown leaves, may be summarized as follows : (1) The presence of red granules of rhodoxanthin (a carotenoid), and a well developed lamellar system involving grana- and intergrana-lamellae. (2) The plastoglobules, osmio- philic granules in plastids, increase in number and size as compared with the chloroplasts in normal green leaves. (3) Shrinkage which is one of the character- istic features of senescent plastids is not observed. (4) RNA content in plastid stroma is almost unchanged throughout an entire leaf stage ranging from normal green to subsequent regreening. Basic structure of the plastids in regreened leaves is quite similar to that in winter leaves, except for some increase of thylakoid membrane and decrease of osmiophilic granules. Accordingly, it is presumed that the plastids appearing in reddish brown leaves are not mere chro- moplasts but those having an incipient nature of the chloroplast, since real chromoplasts can not be converted into the chloroplasts. -
Malate- and Pyruvate-Dependent Fattyacid Synthesis In
Plant Physiol. (1992) 98, 1233-1238 Received for publication July 9, 1991 0032-0889/92/98/1 233/06/$01 .00/0 Accepted October 10, 1991 Malate- and Pyruvate-Dependent Fatty Acid Synthesis in Leucoplasts from Developing Castor Endosperm' Ronald G. Smith*2, David A. Gauthier, David T. Dennis3, and David H. Turpin Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 ABSTRACT or leucoplast glycolytic enzymes are more than adequate to endosperm Leucoplasts were isolated from the endosperm of developing account for the triacylglycerol synthesis of castor castor (Ricinis communis) endosperm using a discontinuous Per- (17). However, glycolytic intermediates have not been well coil gradient. The rate of fatty acid synthesis was highest when characterized as carbon sources for fatty acid synthesis (18). malate was the precursor, at 155 nanomoles acetyl-CoA equiva- In this study, we present data regarding the kinetics and lents per milligram protein per hour. Pyruvate and acetate also exogenous cofactor requirements for fatty acid synthesis in were precursors of fatty acid synthesis, but the rates were ap- isolated leucoplast preparations using pyruvate, malate, and proximately 4.5 and 120 times less, respectively, than when acetate as carbon sources. We show that malate and pyruvate malate was the precursor. When acetate was supplied to leuco- support much higher rates of fatty acid synthesis than does plasts, exogenous ATP, NADH, and NADPH were required to acetate and the metabolism of both these substrates alleviates obtain maximal rates of fatty acid synthesis. In contrast, the any requirement for externally added reductant. Based on the incorporation of malate and pyruvate into fatty acids did not measurement of leucoplast-associated NADP+-malic enzyme require a supply of exogenous reductant. -
The Pseudopodia of the Leucocytes of Invertebrates
PSEUDOPODIA OF THE LEUCOCYTES OF INA^EBTDBEATES. 19 The Pseudopodia of the Leucocytes of Invertebrates. By Edwin S. Ooodricli, F.R.S., Fellow of Merton College, Oxford. With Plates 1 and 2. IT is to Wharton Jones that we owe the first comprehensive account of the leucocytes in the various groups of Inverte- brata: in an important paper, published in 1846 (6), he described and figured them as of "stellate appearance/' and sometimes as shooting out "cilia-like processes." Since then numberless authors have studied these cells and pictured them as provided with free outstanding pseudopodia. Evidence is brought forward in this paper that the spiny appearance of leucocytes is of the nature of an optical illusion or due to changes taking place under abnormal conditions, that freely projecting fine processes are seldom, if ever, produced in the fluids of living invertebrates, and that the pointed pseudo- podia so often figured are merely the optical sections of deliciite, more or less folded films. Various authors have from time to time mentioned the flattened expanded shape of the processes of the leucocytes of the inv- rtebrates, as, for instance, Cattaneo (1) and Dek- hiu'M'ii (2), referred to below ; but none seem to have realised thai i his is ihe normal shape of the pseudopodia. In 1898 (4) I drew attention to the presence of membranous expansions of the lriicocytes in the Polychaste worm G-lycera, ahd at that tim<" observed the same structures in other invertebrates; but 20 EDWIN S. GOODRICH. other work prevented my pursuing the subject, and it was not till last winter that it was again taken up, when I had the opportunity of confirming and extending my observations at the Marine Biological Laboratory in Plymouth. -
A Contribution to the Life History of Spongospora Subterránea
A CONTRIBUTION TO THE LIFE HISTORY OF SPONGOSPORA SUBTERRÁNEA [A PRELIMINARY REPORT] By L. O. KUNKEL, Pathologist, Office of Cotton- and Truck-Disease Investigations, Bureau of Plant Industry INTRODUCTION The manner of infection of tubers of the potato (Solatium tuberosum) by Spongospora subterránea seems never to have been observed. Osbora (14)1 has described and figured what he considers a single amœba in a potato cell, but as will be seen from the writer's descriptions this can not be regarded as a significant step in the process of infection. It has usually been assumed that each potato cell becomes parasitized by one, or at most only a very few, amoebae in much the same way as suggested by Woronin (17) for cabbage cells attacked by Plasmodiophora brassicae. Spongospora subterránea was first technically described by Wallroth (16) in 1842 as Erysibe subterránea. Although it seems to have been widely distributed in Europe, it received little attention until 1885, when Brun- chorst (2), a Norwegian botanist, made a careful study of the disease. His observation of plasmodia in the potato cells led him to place it in the group of the Plasmodiophoraceae. It was his opinion that the organism could live saprophytically in the soil, and he states that it is a general belief among farmers that lime and excess moisture favor the disease. In 1892 Von Lagerheim (9) reported 5. subterránea as very generally distributed in Quito, South America, which is the native habitat of the potato and may also be the home of Spongospora. Johnson (7) studied the germination of the spores and describes eight swarm spores coming from each cell of the spore ball. -
Revised ST.26 – 26/Oct/2016 ANNEX I
Revised ST.26 – 26/Oct/2016 ANNEX I ST.26 - ANNEX I CONTROLLED VOCABULARY Final Draft TABLE OF CONTENTS SECTION 1: LIST OF NUCLEOTIDES ................................................................................................................................. 2 SECTION 2: LIST OF MODIFIED NUCLEOTIDES .............................................................................................................. 2 SECTION 3: LIST OF AMINO ACIDS ................................................................................................................................... 4 SECTION 4: LIST OF MODIFIED AND UNUSUAL AMINO ACIDS ..................................................................................... 5 SECTION 5: FEATURE KEYS FOR NUCLEIC SEQUENCES ............................................................................................ 6 SECTION 6: QUALIFIERS FOR NUCLEIC SEQUENCES ................................................................................................ 21 SECTION 7: FEATURE KEYS FOR AMINO ACID SEQUENCES ..................................................................................... 40 SECTION 8: QUALIFIERS FOR AMINO ACID SEQUENCES ........................................................................................... 47 SECTION 9: GENETIC CODE TABLES ............................................................................................................................. 48 Revised ST.26 – 26/Oct/2016 ANNEX I SECTION 1: LIST OF NUCLEOTIDES The nucleotide base codes to be used in sequence -
ZOOLOGY Biology of Parasitism Morphology, Life Cycle
Paper : 08 Biology of Parasitism Module : 18 Morphology, Life cycle, Pathogenecity, Diagnosis and Prophylaxis of Entamoeba Part 1 Development Team Principal Investigator : Prof. Neeta Sehgal Department of Zoology, University of Delhi Co-Principal Investigator : Prof. D.K. Singh Department of Zoology, University of Delhi Paper Coordinator : Dr. Pawan Malhotra ICGEB, New Delhi Content Writer : Dr. Ranjana Saxena Dyal Singh College, University of Delhi Content Reviewer : Prof. Rajgopal Raman Department of Zoology, University of Delhi 1 Biology of Parasitism ZOOLOGY Morphology, Life cycle, Pathogenecity, Diagnosis and Prophylaxis of Entamoeba Part 1 Description of Module Subject Name ZOOLOGY Paper Name Biology of Parasitism; Zool 008 Module Name/Title Protozoans Module Id M18: Morphology, Life cycle, Pathogenecity, Diagnosis and Prophylaxis of Entamoeba Part 1 Keywords Trophozoite, precyst, cyst, chromatoidal bars, excystation, encystation, metacystictrophozoites, amoebiasis, amoebic dysentery, extraintestinalinvasion. Contents 1. Learning Outcomes 2. Introduction 3. History of Entamoeba 4. Classification of Entamoeba 5. Geographical distribution of Entamoeba histolytica 6. Habit and Habitat 7. Host 8. Reservoir 9. Morphology 10. Life cycle 11. Transmission 12. Entamoeba dispar 13. Entamoeba gingivalis 14. Entamoeba coli 15. Entamoeba hartmanni 16. Comparison between the various Entamoeba 17. Summary of Entamoeba histolytica 2 Biology of Parasitism ZOOLOGY Morphology, Life cycle, Pathogenecity, Diagnosis and Prophylaxis of Entamoeba Part 1 1. Learning Outcomes After studying this unit you will be able to: Classify Entamoeba Understand the medical importance of Entamoeba Distinguish between the different species of Entamoeba Identify the pathogenic species of Entamoeba Describe the morphology ofEntamoeba histolytica Explain the life cycle of Entamoeba histolytica Compare the life cycle of different species of Entamoeba 2.