11 Review Article Page 1 of 11 Narrative review of production of antioxidants and anticancer compounds from Bryophyllum spp. (Kalanchoe) using plant cell tissue culture Eva Lozano-Milo1,2#, Pascual García-Pérez1,2#, Pedro P. Gallego1,2 1Applied Plant & Soil Biology, Plant Biology and Soil Science Department, Biology Faculty, University of Vigo, Pontevedra, Spain; 2CITACA—Agri- Food Research and Transfer Cluster, University of Vigo, Ourense, Spain Contributions: (I) Conception and design: All authors; (II) Administrative support: PP Gallego; (III) Provision of study materials or patients: All authors; (IV) Collection and assembly of data: All authors; (V) Data analysis and interpretation: All authors; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. #These authors contributed equally to this work. Correspondence to: Pedro P. Gallego. Applied Plant & Soil Biology, Plant Biology and Soil Science Department, Biology Faculty, University of Vigo, Pontevedra E-36310, Spain; CITACA—Agri-Food Research and Transfer Cluster, University of Vigo, Ourense E-32004, Spain. Email:
[email protected]. Abstract: For centuries, plants have been widely used in traditional medicine worldwide for the treatment of many diseases. The subgenus Bryophyllum (genus Kalanchoe) have been used in ethnobotanic medicine across America, Africa and Asia. Traditionally, some formulations derived from leaves and roots of Bryophyllum spp. have been applied for the treatment of common illness such as coughs, fever, infections, insect bites, wounds and burns. Phenolic compounds and bufadienolides are the two major families of secondary metabolites identified in several species of the subgenus Bryophyllum. These compounds have gained much attention due to their associated antioxidant and cytotoxic activity, but they are synthetized by plants in fairly limited amounts.