Histomorphochemical Characterization of Meibomian and Ciliary Glands of Sheep (Ovis Aries)

HV-49-2020 Haryana Vet. (December, 2020) 59(2), 300-301 Clinical Article

OBSTETRICAL MANAGEMENT OF POSTPARTUM TOTAL UTERINE PROLAPSE BY COELIOTOMY AND SINGLE DOSE OF CEFOVECIN SODIUM IN A QUEEN CAT S. RANGASAMY, G. MONICA*, APARNA GOPINADHAN, N. NEELADEVI and U.S. KALYAAN Department of Veterinary Gynaecology and Obstetrics, Madras Veterinary College, Chennai-600 007 Tamilnadu Veterinary and Animal Sciences University Received: 27.04.2020; Accepted: 26.05.2020 SUMMARY The present paper describes the postpartum total uterine prolapse in a queen cat and its management by coeliotomy and single dose administration of injection Cefovecin sodium subcutaneously. Fig. 1. Lacerated total uterine prolapsed mass Fig. 2. Repositioned mass presented intra abdominally Fig. 3. Ovariohyterectomized total Keywords: Cat, Cefovecin sodium, Ovariohysterectomy, Postpartum, Total uterine prolapse uterine mass Total uterine prolapse is uncommon in cats. Uterine mg/kg BW @ 4:1 ratio intravenously. The prolapsed mass maintained for approximately 7 days. One antibiotic concurrent with cystocele in a queen. Iran. J. Vet. Surg. 12(1): 69-73. prolapse occurs when the uterus turns inside out and passes and surgical site was aseptically prepared and the apex of injection Cefovecin sodium given by subcutaneous route MacPhail, C.M. (2013). Surgery of the reproductive and genital through the cervix into the vagina (Deroy et al., 2015) but the uterine horns were lubricated with liquid paraffin. The provides up to 14 days of antibiotic therapy, which in many th systems. In: Small Animal Surgery. (4 Edn.) Fossum, T.W., can occur without mucosal eversion (Bigliardi et al., mass was replaced through the vagina (Fig. 2). A cases eliminates the need for owners to give their pets Dewey, C.W., Horn, C.V., Johnson, A.L., Radlinsky, M.G., 2014). Complete uterine prolapse is reported in cats aged coeliotomy (Fig. 3) was performed and the ovariohyster- medications at home. It is aqueous, non-depot injection for Schulz, K.S. and Willard, M.D. (Edts.). St. Louis: Elsevier between 10 months to 6 years (Ucmak et al., 2018) and the ectomy was done as per the standard procedure using PGA rapid release and it provides sustained, uninterrupted Mosby, pp. 826-827. incidence is less than 0.03% (Senna et al., 2015). It is (2-0). Surgical incision was closed by lockstitch suturing therapeutic drug concentrations. It gives owners peace of Sathiamoorthy, T., Raja, S., Thirumurugan, K. and Kulasekar, K. (2011). Unilateral uterine horn prolapse with retained fetus in a mostly as a sequel of dystocia and increased straining and of muscular layer followed by intra dermal closure using mind that their pets are receiving the treatment they need queen cat. Indian Vet. J. 88(9): 120-121. can occur instantly or up to 48 hours after delivery of the PGA (2-0). Post-operatively, the queen was administered a without the stress of administering daily oral medications. Senna, N.A., Farghali, H.A. and Shalaby, R.K.I. (2015). Some studies last kitten or after a prolonged queening or abortion single dose of Inj. Cefovecin sodium @ 8 mg/kg BW S/C. Thus prompt and quick decision on the treatment aspect of on feline genital system surgical affections (prevalence, (Jarolmasjed, 2017). The condition has been reported Since the animal had reduced milk secretion, kittens were total uterine prolapse renders good prognosis pertaining to histopathology and surgical interventions). Academic J. Anim. during the process of queening in a queen (Ucmak et al., fed with milk replacers and the queen recovered the animal’s livability. Dis. 4: 60-68. 2018) and in a non-pregnant queen (Valentine et al., 2015). uneventfully. REFERENCES Ucmak, Z.G., Ucmak, M., Cetin, A.C. and Tek, C. (2018). Uterine prolapse in a pregnant cat. Turkish J. Vet. Ani. Sci. 42(5): 500- Bigliardi, E., Ianni, F.D., Parmigiani, E., Cantoni, A.M. and Bresciani, In the present case, a two year old queen cat weighing In bitches, the severity of clinical signs and 502. C. (2014). Complete uterine prolapse without uterine mucosal 2.8 kg was presented with a large pink mass protruding prognosis depend on the duration of the prolapse Valentine, M.J., Porter, S., Chapwanya, A. and Callanan, J.J. (2015). eversion in a queen. J. Small Anim. Prac. 55(4): 235-237. through the vulva after labour. She had delivered six (Sathiamoorthy et al., 2011). Described methods of Uterine prolapse with endometrial eversion in association with Deroy, C., Bismuth, C. and Carozzo, C. (2015). Management of a complete an unusual diffuse, polypoid, fibrosing perimetritis and kittens on the previous night and was brought the next day treatment include external hysterectomy, manual uterine prolapse in a cat. J. Feline Med. Surg. Open Reports. parametritis in a cat. J. Feline Med. Surg. Open Reports. 2(1): morning with a mild lacerated total uterine prolapse mass. reduction through a laparotomy incision followed by 1(1): 2055116915579681. 2055116915626166. On presentation, the cat was dehydrated and did not have ovariohysterectomy, reduction and repositioning of the Jarolmasjed, S. (2017). Unusual case of utero-ovarian prolapse milk secretion. On clinical examination, heart rate, pulse prolapsed organ by abdominal palpation and use of an rate and respiratory rate were within normal limits. infusion (MacPhail, 2013). Uterine prolapse should be Obstetrical examination revealed complete total uterine considered an emergency and treatment should be done prolapsed mass (Fig 1). The uterus did not have any fetus immediately to prevent infection. Urethral catheterization and it was observed that there was a rupture of the should be done to prevent damage to the urethra during mesovarium, mesometrium and utero-vaginal connection hysterectomy (Deroy et al., 2015). Prognosis improves if around the cervix. Due to extensive damage of the uterus treatment is instituted rapidly and is excellent following and the torn ligaments, manual reduction of the prolapsed ovariohysterectomy, if hemorrhage and shock are treated uterus was not possible and an emergency ovariohyste- accordingly. rectomy was performed. The present case describes the reduction of the The animal was administered with Inj. Xylazine @ 1 prolapsed mass carefully by repositioning it followed by mg/kg I/M and Inj. Ketamine @ 5 mg/kg BW I/M as the performing ovariohysterectomy. Post operatively single pre-anaesthetics and the anaesthesia was maintained with dose Inj. Cefovecin sodium was administered as a single, Inj. Ketamine @ 5 mg/kg BW and Inj. Diazepam @ 2 one time subcutaneous injection at a dose of 3.6 mg/lb (8 mg/kg) body weight and its therapeutic concentration are *Corresponding author: [email protected]

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THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the evaluation of articles. We request them to continue to CONTRIBUTORS MAY NOTE extend their co-operation and be prompt in future to give

l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians CONTRIBUTORS MAY NOTE their valuable comments on the articles for timely engaged in Veterinary Profession. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarianspublication of the journal. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. engaged in Veterinary Profession. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Veterinary Sciences, along with each article. writing/submission. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of l Please mention your article reference number in all correspondence for a quick Veterinary Sciences, along with each article. response. l After revision, please return the revised manuscript and rebuttal at the earliest. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation. Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors

Marinelli, L., Adamelli, S., Normando, S. and Bono, G. (2007). Quality of life of the pet dog: Influence of owner and dog’s characteristics. Appl. Anim. Behav. 1 2 Sci. 108(1-

Corresponding author: [email protected] trus synchronizathod that synchronizes ovulations is named briefly as “Ovsynch” (Pursley et al., 1995). The study was aimed to evaluate the efficacy of different methods of estrus sync HV-28-2020 Haryana Vet. (June, 2020) 59(1), 154-155 Clinical Article

VAGINAL LEIOMYOMA IN A HOLSTEIN FRIESIAN COW: A CASE REPORT A. A. MALIK1*, R. HABIB1, T. MUSHTAQ1 , P. GOSWAMI2 and D. RASHID1 1Division of Animal Reproduction, Gynaecology and Obstetrics, 2Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST- K, Srinagar-190006, India Received: 09.03.2020; Accepted: 15.04.2020 Research Article Haryana Vet. (December, 2020) 59(2), 160-163 RESULTS AND DISCUSSION The meibomian glands of sheep were of compound SUMMARY Fig. 1. Fetal monster showing cyclopia and arhinia Histologically, the eyelids consisted of four main tubuloalveolar type. These glands were modified A four year old Holstein Friesian cow in her second parity was presented with the history of vaginal mass and little vaginal bloody discharge HISTOMORPHOCHEMICAL CHARACTERIZATION OF MEIBOMIAN AND CILIARY sebaceous glands which reside within the upper and lower from past ten days. Per vaginal examination confirmed the presence of small mass prolapsed through vulva. The growth was excised and sent for GLANDS OF SHEEP (OVIS ARIES) layers, from exterior to interior, viz., skin (epidermis and dermis), muscular layer, tarsal layer and palpebral eyelids. These glands were more numerous and better histopathological examination. Based on arrangement of collagen fibres and interlocking bundles of connective tissue fibres and cells, the growth was interpreted as collagenous leiomyoma and was surgically excised without any post-operative complication. AMANDEEP SINGH*, PARVEEN KUMAR GAHLOT and TEJ PARKASH conjunctiva. The epidermal layer comprised of stratified developed in the upper eyelids than the lower one. These Keywords: Histopathology, Holstein Friesian cow, Leiomyoma, Vagina tumor Department of Veterinary Anatomy, College of Veterinary Sciences squamous keratinized epithelium, and a dermis of loose linear arrays of sebaceous glands resembled a grape-like LUVAS, Hisar- 125 004, India collagenous connective tissue along with hair follicles. clusters attached to a central stalk (Fig. 3). These large Received: 03.01.2020; Accepted: 06.02.2020 Associated with these hair follicles, were small sebaceous multilobulated glands were arranged in the tarsal plate, a thick plaque of dense fibrous connective tissue (Fig. 1a). ABSTRACT (Zeis) glands and modified apocrine sweat (Moll) glands The posterior palpebral margins in the red kangaroo and The present study was conducted on five healthy young sheep of local mixed breed of either sex to study the histomorphology and (Fig. 1a). Similar findings were reported in mammals (McGavin histochemistry of the meibomian and ciliary glands of sheep. The meibomian glands were of compound tubuloalveolar type consisting of linear et al., 2001). However, the porcine upper eyelid contained other mammals are lined with multiple tarsal glands which arrays of sebaceous glands in the tarsal plates of the eyelids; however the ciliary glands were of simple coiled tubular type. The lobules of the prominent apocrine ciliary glands, sebaceous glands and appear as yellow columns positioned parallel to one meibomian glands consisted of clusters of secretory glandular cells (meibocytes) of various sizes, resting on a basement membrane surrounded by a less-abundant tarsal glands (Yasui et al., 2006). The another and perpendicular to the eyelid margins (Nasrin et thin connective tissue sheath. The meibocytes have a vacuolar cytoplasm and their oval nuclei were situated at centre of the cell. The peripheral al., 2013). However, the camel eyelids showed an ill- meibocytes were smaller, whereas central cells were larger and polyhedral in nature. The meibocytes were connected to a central duct, lined by muscular layer was comprised of fascicles of striated muscle stratified squamous epithelium, which directly opened at the muco-cutaneous junction of the eyelid. The secretory portion of the ciliary glands fibres of orbicularis oculi muscle. Next to this layer, was developed tarsus at the center of the eyelid which is comprised of large irregular lumen lined with cuboidal epithelial cells having eosinophilic cytoplasm. The apical surface of these cells showed the tarsal plate, which contained large sebaceous, tarsal infiltrated by some bundles of connective tissue (Saeed, varying cytoplasmic protrusions; however, the ducts of these glands were lined by stratified cuboidal epithelium and were directly associated with (meibomian) glands with alveoli emptying into long 2015). The lobules were composed of clusters of secretory hair follicles. Histochemical studies revealed the absence of neutral mucopolysaccharides, weakly acidic sulfated mucosubstances, sialomucins and glandular cells (meibocytes) of various sizes (Fig. 1b), hyaluronic acid in the secretory units of both meibomian and ciliary glands. central ducts that opened at the free edge of the eyelids resting on a basement membrane which was surrounded by Keywords: Ciliary gland, Histology, Histochemistry, Meibomian gland, Sheep (Fig. 1b). While in avian eyelids sebaceous glands are absent *Corresponding author: [email protected] but the epidermal epithelium as a whole produces the a thin connective tissue sheath. The meibocytes have a The meibomian gland, also known as tarsal gland, is meibomian and ciliary glands has been studied in brown phospholipids and neutral fats usually produced by these light pink (acidophilic) cytoplasm, and the vacuoles were a holocrine type of exocrine gland, located at the rim of the brocket deer (Ajmat et al., 2004), calf (Yuksel et al., 2005), glands (Stettenheim, 2000). The conjunctiva (palpebral) filled with a homogenous eosinophilic material. The upper and lower eyelids inside the tarsal plate, responsible pig (Yasui et al., 2006), African black ostrich lined the inner surface of the eyelids, where the epithelium connective tissue sheath was mainly made up of collagen for the supply of meibum, an oily substance that prevents (Kleckowska-Nawrot et al., 2016), there is a lack of was gradually changed from stratified squamous to fibres and blood vessels of varying sizes (Fig. 4). These evaporation of the eye’s tear film. Dysfunctional literature on the light microscopic details of the pseudostratified columnar having numerous goblet cells. findings were in fully agreement with the reports in guinea meibomian glands often cause dry eyes, one of the most meibomian and ciliary glands in young sheep and its Lymphatic nodules were located in the subconjunctival pig (Gasser et al., 2011) and camel (Saeed, 2015). The oval common eye conditions. The ciliary glands, also known as comparison with other domestic animals. Keeping in view tissue towards the palpebral conjunctiva and these were nuclei of meibocytes were situated at centre of the cell. The glands of Moll, function is unknown. Moll glands have the importance of these glands, the present study describes termed as conjunctiva-associated lymphoid tissue (CALT) peripheral meibocytes were smaller, whereas central cells apocrine secretion and leave their secretions inside the the histological and histochemical findings of Meibomian (Fig. 2). CALT seems to play an important role in the were larger and polyhedral in nature (Fig. 1b). Similar eyelash follicles (McGavin et al., 2001). The tear film and ciliary glands in young sheep. protection of ocular surface by initiating and regulating findings were reported in guinea pig (Gasser et al., 2011). comprises of three layers: the superficial lipid layer MATERIALS AND METHODS immune responses (Steven and Gebert, 2009). Saeed The meibocytes were connected to a central excretory produced by the meibomian glands and Zeis glands, the duct, lined by stratified squamous epithelium, which The upper and lower eyelids used in the present (2015) reported that the several lymphoid follicles were Fig. 1 & 2. (1) Hypoechoic areas of pus inside uterus (yellow arrow) and middle aqueous layer derived from the major lacrimal directly opened at the muco-cutaneous junction of the increased endometrial thickness evident of cystic endometrial study were obtained from five healthy young sheep of local present on the palpebral surface of the eyelids of camel, glands and the accessory lacrimal glands of Krause and eyelid (Fig. 1b). The tarsal plate was covered externally by hyperplasia (orange arrow) before treatment with methylergometrine. mixed breed of either sex. The heads were procured from specifically near the medial canthus. Kleckowska-Nawrot (2) Clear uterus with no hypochoic appearance of pus (yellow arrow) Wolfring, and the inner mucinous layer predominantly skeletal muscle fibers and internally by the palpebral local slaughter house immediately after decapitation and et al. (2016) also showed the presence of organized lymphoid and reduced thickness of endometrium (orange arrow) after treatment from the goblet cells of the conjunctiva (Argueso and conjunctiva having goblet cells. Kleckowska-Nawrot et al. with methylergometrine the tissues were fixed in a 10% neutral buffered formalin follicles only in the lower eyelid of red kangaroo. Organized Gipson, 2001). So far, a contribution to the tear film from (2016) also showed the presence of goblet cells in the solution for 48 hours, subjected to routine tissue processing conjunctiva-associated lymphoid tissue, consisting of a the ciliary glands and from the surface epithelium is not palpebral and bulbar conjunctiva zones of both eyelids, but for light microscopic examination and embedded in paraffin well-developed lymphoid follicle in intimate contact with mentioned in the literature, however; the glandular palpebral epithelium, was reported in mammals such as numerous goblet cells were identified in the palpebral blocks. The paraffin sections (5-6 m) were made through secretions of the porcine ciliary glands may play an humans (Knop and Knop, 2000) and rabbits (Liu et al., conjunctiva zone of the lower eyelid of red kangaroo. *Corresponding author: [email protected] the upper and lower eyelids of left as well as right eyes and essential role in the preservation of humidity and the 2005). In birds, the CALT and the Harderian gland form Saeed (2015) reported that the palpebral conjunctiva of the stained with routine Harris’ hematoxylin and eosin stain for protection against environmental pathogens not only in the the head-associated lymphoid tissue (HALT) (van Ginkel dromedary camel was lined with epithelia that varied from 154 general histomorphological examination, Gomori’s stain eyelid skin, but also on the ocular surface (Yasui et al., et al., 2012). CALT was also described as solitary or stratified squamous to stratified columnar, however, for reticular fibres, Weigert’s method for elastic fibres (Luna, 2006). In certain mammals, recent studies have aggregate lymphoid follicles in Angora goats, Japanese goblet cells were present in all forms of epithelia except 1968) and Crossman’s trichrome stain for collagen fibres demonstrated that the apocrine glandular secretions monkeys and domestic animals (Cain and Philips, 2008). over the lymphoid follicles, where the epithelium was (Crossman, 1937). In addition, selected sections were processed contain antimicrobial substances such as lysozyme and Together with the orbital glands and the CALT they transformed to low stratified squamous. for histochemical demonstration of mucopolysaccharides defensins (Yasui et al., 2006). moisturize the eye and supply the cornea and conjunctival Histochemically, a periodic acid-Schiff (PAS) negative using McManus’ method, Periodic Acid Schiff-Alcian Although, the histology and histochemistry of the sacs with nutrients and antibodies to maintain their health reaction was observed in the secretory units of the gland blue method, Alcian blue (pH 2.5) method, Colloidal iron (Jochems and Philips, 2015). indicating the absence of neutral mucopolysaccharides. *Corresponding author: [email protected] method and Mayer’s mucicarmine method (Luna, 1968).

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CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. CONTRIBUTORS MAY NOTE Editors l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/ submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar- 125004. THE HARYANA VETERINARIAN Editors Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give their valuable comments on the articles for timely publication of the journal.

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Marinelli, L., Adamelli, S., Normando, S. and Bono, G. (2007). Quality of life of the pet dog: Influence of owner and dog’s characteristics. Appl. Anim. Behav. 1 2 Sci. 108(1-

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Marinelli, L., Adamelli, S., Normando, S. and Bono, G. (2007). Quality of life of the pet dog: Influence of owner and dog’s characteristics. Appl. Anim. Behav. 1 2 Sci. 108(1-

follicles, were small sebaceous (Zeis) glands (Fig. 1a). The Cain, C. and Phillips, T.E. (2008). Developmental changes in SUMMARY secretory portion was comprised of large irregular lumen conjunctiva- associated lymphoid tissue of the rabbit. Invest. Fig. 1. Fetal monster showing cyclopia and arhinia Ophthalmol. Vis. Sci. 49: 644-649. A four year old Holstein Friesian cow in her second parity was presented with the history of vaginal mass and little vaginal bloody discharge and lined with cuboidal epithelial cells having eosinophilic from past ten days. Per vaginal examination confirmed the presence of small mass prolapsed through vulva. The growth was excised and sent for Crossman, G.A. (1937). A modification of Mallory’s connective tissue histopathological examination. Based on arrangement of collagen fibres and interlocking bundles of connective tissue fibres and cells, the growth cytoplasm (Fig. 7). These findings were in fully agreement stain with a discussion of principles involved. Anat. Rec. 69: 33- with those reported in pig (Yasui et al., 2006). The apical 38. was interpreted as collagenous leiomyoma and was surgically excised without any post-operative complication. surface of the cells showed varying cytoplasmic protrusions. Gasser, K., Fuchs Baumgartinger, A., Tichy, A. and Nell, B. (2011). Keywords: Histopathology, Holstein Friesian cow, Leiomyoma, Vagina tumor These glands were separated from each other with a septa Investigations on the conjunctival goblet cells and on the composed of connective tissue fibres and vascular tissue. characteristics of glands associated with the eye in the guinea pig. Vet. Ophthalmol. 14: 26-40. 1a 1b 2 The ducts of these glands were lined by stratified cuboidal epithelium and were connected with hair follicles. Jochems, B. and Phillips, T.E. (2015). Histological and ultrastructural studies on the conjunctiva of the Barred Owl (Strix varia). PLoS Histochemical studies revealed the absence of both acidic One. 10: e0142783. https://doi.org/10.1371/journal.pone. and neutral mucopolysaccharides in secretory cells by 0142783. PAS-Alcian blue method. The reaction intensities of the Kleckowska-Nawrot, J., Gozdziewska-Har³ajczuk, K. and Nowaczyk, secretory epithelium and luminal secretion were generally R. (2016). Morphological study of the upper, lower and third weak as reported by Yasui et al. (2006) in pig. The eyelids in the African black ostrich (Struthio camelus camelus) secretory units of the gland also showed the absence of during the embryonic and postnatal period. Ital. J. Zool. 83: 312- 328. neutral mucopolysaccharides by McManus’ method. Also, Knop, N. and Knop, E. (2000). Conjunctiva-associated lymphoid tissue these secretory cells showed negative reaction to Alcian in the human eye. Invest. Ophthalmol. Vis. Sci. 41: 1270-1279. 3 4 5 blue (pH 2.5) and Mayer’s mucicarmine methods. Liu, H., Meagher, C.K., Moore, C.P. and Phillips, T.E. (2005). M cells in It may be concluded that the dermal layer of the the follicle-associated epithelium of the rabbit conjunctiva *Corresponding author: [email protected] eyelids was comprised of loose collagenous connective preferentially bind and translocate latex beads. Invest. tissue that contains hair follicles and associated unilobular Ophthalmol. Vis. Sci. 46: 4217-4223. Luna, L.G. (1968). Manual of Histologic Staining methods of Armed sebaceous glands (Zeis glands), modified multilobular rd Forces Institute of Pathology (3 Edn.), McGraw Hill Book Co., sebaceous glands (meibomian or tarsal glands) and New York. apocrine sweat glands (Moll or ciliary glands). The large McGavin, M.D., Carlton, W.W. and Zachary, J.F. (2001). Thomson’s meibomian glands were arranged as separate, single Special Veterinary Pathology (3rd Edn.), Mosby Inc., St. Louis, strands in parallel arrangement in the tarsal plates of the Missouri. 6 7 both eyelids. The secretory cells (meibocytes) emptied Nasrin, M.K., Khan, M.Z.I., Siddiqi, M.N.H. and Masum, M.A. (2013). into a large central duct, which directly opened at the Mobilization of immunoglobulin (Ig)-containing plasma cells in Figs. 1-7. 1a. Photomicrograph of eyelid of sheep showing various histological layers (E- epidermis; D- dermis; H- hair follicles; Z- zeis Harderian gland, cecal tonsil and trachea of broilers vaccinated glands; Cg- ciliary/Moll glands, Mf- muscle fibres of orbicularis oculi; Mg- meibomian/tarsal glands; Cd- central duct; J- muco-cutaneous muco-cutaneous junction of the eyelids. The ciliary glands with Newcastle Disease vaccine. Tissue Cell. 45: 191- 197. junction; C- conjunctiva) (H & E x 40). 1b. Photomicrograph showing large sized multilobular sebaceous (meibomian) glands of sheep eyelid were modified apocrine sweat glands and located at the with alveoli (A) emptying into long central duct (Cd) that opened at the muco-cutaneous junction (J) (H & E x 100). 2. Photomicrograph of eyelid Saeed, Y. AL-Ramadan. (2015). Histological features and mucidistribution of sheep showing the palpebral conjunctiva (C) lined the inner surface of the eyelids. The epithelium gradually changed from stratified squamous base of the hair follicles. The secretory portion of the in the palpebral conjunctiva of the dromedary camel (Camelus to pseudostratified columnar having numerous goblet cells (arrow). Lymphatic nodules (Ln) were also seen in the subconjunctival tissue towards the ciliary glands was comprised of large irregular lumen and dromedarius) Assiut. Vet. Med. J. 61: 179-186. Fig. 1 & 2. (1) Hypoechoic areas of pus inside uterus (yellow arrow) and palpebral conjunctiva (H & E x 40). 3. Photomicrograph of eyelid of sheep showing the linear arrays of meibomian glands (Mg) arranged in grape- increased endometrial thickness evident of cystic endometrial like clusters attached to a central stalk (Cs) (H & E x 100). 4. Photomicrograph of eyelid of sheep showing the connective tissue sheath which was lined with cuboidal epithelial cells having eosinophilic Stettenheim, P.R. (2000). The integumetary morphology of modern mainly made up of collagen fibres (Cf) and blood vessels of varying sizes (Crossman Trichrome x 40). 5. Photomicrograph of eyelid of sheep showing cytoplasm. Histochemical studies revealed the absence of birds - An overview. Amer. Zoolog. 40: 461-477. hyperplasia (orange arrow) before treatment with methylergometrine. (2) Clear uterus with no hypochoic appearance of pus (yellow arrow) the strong PAS positive reaction in the goblet cells (arrow) of the palpebral conjunctiva indicating the presence of more amounts of neutral neutral mucopolysaccharides, weakly acidic sulfated Steven, P. and Gebert, A. (2009). Conjunctiva-associated lymphoid mucopolysaccharides (PAS x 40). 6. Photomicrograph of eyelid of sheep showing a negative PAS-AB reaction for both neutral and acid and reduced thickness of endometrium (orange arrow) after treatment mucopolysaccharides in the secretory units; however, the goblet cells (arrow) showed the presence of these substances (PAS-AB x 40). 7. mucosubstances, sialomucins and hyaluronic acid in the tissue - Current knowledge, animal models and experimental with methylergometrine Photomicrograph of eyelid of sheep showing the Zeis glands (Z) and modified apocrine (ciliary) glands (Cg). The secretory portion of ciliary glands secretory units of both Meibomian and ciliary glands. prospects. Ophthalmic Res. 42: 2-8. comprised of large irregular lumen and lined with cuboidal epithelial cells having eosinophilic cytoplasm (H & E x 100). van Ginkel, F.W., Gulley, S.L., Lammers, A., Hoerr, F.J., Gurjar, R. and ACKNOWLEDGEMENT However, a strong PAS positive reaction was seen in the revealed negative reaction for both neutral and acid Toro, H. (2012). Conjunctiva-associated lymphoid tissue in avian mucosal immunity. Dev. Comp. Immunol. 36: 289–297. goblet cells of the palpebral conjunctiva indicating the mucopolysaccharides in the secretory units; however the The authors are thankful to Lala Lajpat Rai *Corresponding author: [email protected] presence of more amounts of neutral mucopolysaccharides goblet cells showed the presence of these substances (Fig. 6). University of Veterinary and Animal Sciences (LUVAS), Voigt, S., Fuchs-Baumgartinger, A., Egerbacher, M., Tichy, A. and Nell, Hisar for providing all type of facilities to carry out the B. (2012). Investigations on the conjunctival goblet cells and the (Fig. 5). These results were in accordance with the findings Mayer’s mucicarmine showed negative reaction which characteristics of the glands associated with the eye in 154 in camel (Saeed, 2015). In gerbil, however, goblet cells indicated the absence of acid mucopolysaccharides in the study. The funding for this study was provided by College chinchillas. Vet. Ophthalmol. 15: 333-344. were observed either as solitary or in clusters, but their secretory cells, whereas the goblet cells showed the of Veterinary Sciences, LUVAS, Hisar. Yasui, T., Tsukise, A., Nara, T., Kuwahara, Y. and Meyer, W. (2006). distribution was detected throughout the entire conjunctiva presence of same. REFERENCES Morphological, histochemical and immunohistochemical characterization of secretory productions of the ciliary gland of Ajmat, M.T., Chamut, S. and Black, D.P. (2004). A histological study of (Voigt et al., 2012). These glandular units also showed The ciliary glands of sheep were of simple coiled the porcine eyelid. Europ. J. Histochem. 50: 99-108. negative reaction to weakly acidic sulfated mucosubstances, cutaneous glands in the brown brocket deer. Acta Theriol. 49: tubular type. These were modified apocrine sweat glands 93-102. Yuksel, H., Gulbahar, M.Y. and Aslan, L. (2005). Congenital sialomucins and hyaluronic acid with Alcian blue (AB) at synchronous adenomas of meibomian and moll glands of the and were located at the base of the hair follicles, anterior to Argueso, P. and Gipson, I.K. (2001). Epithelial mucins of the ocular eyelid in a calf. Vet. Med. 50: 379–383. pH 2.5, while the unicellular mucous glands showed the Meibomian glands. 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