DOCSLIB.ORG

Multilamellar Bodies Linked to Two Active Plasmalemma Regions in the Pollen Grains of Sarcocapnos Pulcherrima

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Multilamellar Bodies Linked to Two Active Plasmalemma Regions in the Pollen Grains of Sarcocapnos Pulcherrima BIOLOGIA PLANTARUM 57 (2): 298-304, 2013 DOI: 10.1007/s10535-012-0295-8 Multilamellar bodies linked to two active plasmalemma regions in the pollen grains of Sarcocapnos pulcherrima M.C. FERNÁNDEZ1*, M.A. PÉREZ-GUTIERREZ2, V.N. SUAREZ-SANTIAGO2, M.J. SALINAS-BONILLO3, and A.T. ROMERO-GARCÍA2 Department of Cell Biology, Faculty of Sciences, University of Granada, E-18071 Granada, Spain1 Department of Botany, Faculty of Sciences, University of Granada, E-18071 Granada, Spain2 Department of Plant Biology and Ecology, University of Almeria, E-04071 Almeria, Spain3 Abstract The presence of visible multilamellar bodies in the cytoplasm of pollen grains of at least seven species of the family Papaveraceae has led us to study the behaviour of these bodies during pollen-grain ontogeny and in growing pollen tubes of Sarcocapnos pulcherrima C. Morales & R. Garcia germinated in vitro. Our transmission-electron-microscope (TEM) studies in pollen grains show that the multilamellar bodies may be classified as: 1) small, isolated and placed in the region of apertures in the cytoplasm; and 2) large, in clusters and in contact with the active plasmalemma apertures only when tubules are being formed in the apertural intine. Similar types of multilamellar bodies to those observed in the pollen apertures can be seen near the apex of the growing pollen tube (small and isolated) and in contact with the apex plasmalemma (large and clustered). Our results support the hypothesis that the multilamellar bodies are functionally linked to moments when the cytoplasmic membrane is very active. We have also linked the multilamellar bodies to Golgi vesicles as they both react positively to acid-phosphatase (AP) staining and also to the plasmalemma by the thiocarbohydrazide-silver proteinate-staining (TCH-Sp) electron-contrasting technique. Additional key words: ontogeny, Papaveraceae, pollen apertures, pollen tubes, TEM, ultrastructure. Introduction Intracellular membrane inclusions have been frequently of Papaveraceae and also within the apex of the pollen described. Due to the numerous locations and conditions tube in Sarcocapnos pulcherrima, germinated in vitro. in which these structures may be found, they have We also found lamellar structures in the form of myelin received various names and have been endowed with bodies in Sarcocapnos pulcherrima pollen tubes. The diverse functions. myelin and multilamellar bodies described here are not In plant cells, multivesicular bodies have been related either in their structure or location. associated with the proteolytic processes in Arabidopsis Pollen apertures play a crucial role in the sexual (Otegui et al. 2006) and the protein bodies of mung bean reproduction of plants and have a very complex structure cotyledons (Van der Wilden et al. 1980). Arabino- which undergoes significant changes during its onto- galactan proteins have been found in Nicotiana tabacum genesis (Fernández and Rodríguez-García 1989, 1995). both in plasmalemmasomes and in multi-lamellar bodies One of the clearest apertural changes observed in some in the pollen tubes as well as in other sub-cellular sites taxa is the differentiation of the apertural intine, involving (Ferguson et al. 1999). a lens-shaped thickening known as the intinous oncus We describe here multilamellar bodies observed in the which plays an important role in germination. The vicinity of apertures in the pollen grains of seven species cytoplasmic membrane below the intinous oncus is very ⎯⎯⎯⎯ Received 31 May 2012, accepted 4 September 2012. Abbreviations: AP - acid-phosphatase; TCH-Sp - thiocarbohydrazide-silver proteinate; TEM - transmission electron microscopy. Acknowledgements: This research was funded by the Spanish Ministry of Science and Innovation, CGL2008-01554/BOS. We thank Dr. Dirk de Meyere of the National Botanical Garden of Belgium and Henrik Zutterlung of the Botanical Garden of Göteborg (Sweden) for their help in the collection of material for analysis in our work. We are grateful for the support of Concepción Hernández Castillo, María José Martínez Guerrero and David Porcel Muñoz of the Scientific Instrumentation Centre of the University of Granada for preparing our samples and TEM visualization. We also thank Angela L. Tate for translating and editing our text. * Author for correspondence: fax: (+ 34) 958 243258, e-mail: [email protected] 298 MULTILAMELLAR BODIES LINKED TO PLASMALEMMA active producing tubules that extend towards the intine The aim of our study was to detect multilamellar and generate a layer of membranous tubules in the bodies in the pollen grains of seven species of the intinous oncus. Initially, the tubules are in contact with Papaveraceae family and in the pollen tubes of the plasmalemma and finally they separate from the Sarcocapnos pulcherrima using transmission electron cytoplasm membrane (Rodríguez-García and Fernández microscope and to classify them according to their size 1988, Fernández et al. 1992). and location. We used acid-phosphatase (AP) staining Another very active area of the cytoplasmic (Megías and Renau 1998) to establish a possible membrane is the apical zone of the pollen tube. This is relationship between the multilamellar bodies and other one of the areas of rapid growth in eukaryotic cells and neighbouring cytoplasmic organelles. To determine the the cytoplasmic membrane of this zone is very active relationships between the plasmalemma and multi- during exocytosis and endocytosis, although the sub- lamellar bodies we also used thiocarbohydrazide-silver cellular location of this growth is as yet unknown. Zonia proteinate-staining (TCH-Sp), an electron-contrasting and Munnik (2008) found that the apex is the area where technique specific to the plasma membrane of plant cells endocytosis and exocytosis occur but that the membrane (Weber 1992). surface area also increases rapidly. Materials and methods Developing anthers and fresh pollen were collected from: (m/v) glutaraldehyde with 0.025 M cacodylate sodium Sarcocapnos pulcherrima C. Morales & R. Garcia buffer (pH 7.2) at room temperature for 24 h. They were (subfamily Fumarioideae, tribe Fumarieae, wild washed with several changes of cacodylate buffer and population sample number GDAC 22851); Hypecoum post-fixed in 1 % (m/v) OsO4 for 2 h. They were then imberbe Sm. (subfamily Hypecoideae, wild population dehydrated in a graded series of ethanol and embedded in sample number GDAC 22824; Fumaria capreolata L. Epon. Ultra-thin sections were cut on a Ultracut E (Leica (subfamily Fumarioideae, tribe Fumarieae, wild Microsystems, Wetzlar, Germany) and stained with 2 % population sample number GDAC 22941; Platystemon (m/v) uranyl acetate and lead citrate (Reynolds 1963). californicum Benth. (subfamily Papaveroideae, tribe Observations were carried out with a Carl Zeiss (Jena Platystemonoideae, living collection accession number Germany) LIBRA 120 Plus transmission electron 20051151-87, National Botanical Garden of Belgium); microscope (TEM). Pseudofumaria alba (Mill.) Lidén (subfamily For AP cytochemistry conducted in Sarcocapnos Fumarioideae, tribe Fumarieae, living collection pulcherrima, we followed Megías and Renau’s (1998) accession number 1985-0760, Botanical Garden of procedure using samples without post-fixation in osmium Göteborg, Sweden; Euptelea poliandra Siebold & Zucc tetroxide. For TCH-Sp cytochemistry in osmium-fixed (family Eupteleaceae, (living collection accession material from Sarcocapnos pulcherrima, we followed number 19723330, National Botanical Garden of Weber’s procedure (1992). Belgium; and Pteridophyllum racemosum (subfamily For in vitro germination, pollen grains of Pteridophylloideae, living collection accession number Sarcocapnos pulcherrima were kept at 25 ºC in 1982-0246, Botanical Garden of Göteborg, Sweden). Brewbaker and Kwack’s medium (1963) and fixed after Anthers of the 7 species in different phases of pollen 1 and 2 h incubation and germination of the pollen tube. maturation were collected. Samples were prefixed in 3 % Results The multilamellar bodies found in the pollen of the seven the oncus was very active producing a large number of species studied were electron dense, irregular in shape, growing tubules that extend towards the intine (Fig. 1B). and dispersed within the cytoplasm, although clearly The tubules were formed from the plasmalemma and within the region of the pollen wall and most frequently groups of large multilamellar bodies could be seen in below the apertural zones (Fig. 1A). They appeared only contact with it (Fig. 1B). The multilamellar bodies were from the intermediate bicellular stage during pollen composed of various peripheral membranous layers ontogeny (Fig. 1), when the generative cell was arranged more or less compactly around an apparently embedded within the pollen grain, until maturity. During empty electron-transparent core (compare Fig. 2A,B,C,D, the intermediate bicellular stage, the intine of the and E). Some of the multilamellar bodies contained only apertural areas thickens into a lens shape forming the compact lamellars and sometimes had a membranous intinous oncus within which a large number of tubules edge (Fig. 2C). either in the process of formation or growth were seen After Sarcocapnos pulcherrima pollen grains were (Fig. 1A). The cytoplasmic membrane in the areas below treated by TCH-Sp staining, the multilamellar bodies 299 M.C. FERNÁNDEZ et al. appearred to be opaque with much deeper staining than isolated multilamellar bodies dispersed throughout the the cytoplasm itself (Fig. 3A,B).
Load more

Recommended publications
  • Assessing Sampling Coverage of Species Distribution in Biodiversity Databases
    1 Assessing sampling coverage of species distribution in biodiversity 2 databases 3 4 Running title: Sampling coverage by box-counting 5 6 Maria Sporbert 1,2 *, Helge Bruelheide 1,2, Gunnar Seidler 1, Petr Keil 1,3, Ute Jandt 1,2, Gunnar 7 Austrheim 4, Idoia Biurrun 5, Juan Antonio Campos 5, Andraž Čarni 6,7, Milan Chytrý 8, János Csiky 9, 8 Els De Bie 10, Jürgen Dengler 2,11,12, Valentin Golub 13, John-Arvid Grytnes 14, Adrian Indreica 15, 9 Florian Jansen 16, Martin Jiroušek 8,17, Jonathan Lenoir 18, Miska Luoto 19, Corrado Marcenò 5, Jesper 10 Erenskjold Moeslund 20, Aaron Pérez-Haase 21, Solvita Rūsiņa 22, Vigdis Vandvik 23,24, Kiril Vassilev 11 25, Erik Welk 1,2 12 13 1Institute of Biology / Geobotany and Botanical Garden, Martin Luther University Halle-Wittenberg, 14 Halle, Germany 15 2German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany 16 3Institute of Computer Science / Biodiversity Synthesis, Martin Luther University Halle-Wittenberg, 17 Halle, Germany 18 4Department of Natural History, University Museum Norwegian University of Science and 19 Technology, Trondheim, Norway 20 5Department Plant Biology and Ecology, University of the Basque Country UPV/EHU, Bilbao, Spain 21 6Scientific Research Centre of the Slovenian Academy of Sciences and Arts, Jovan Hadži Institute of 22 Biology, Ljubljana, Slovenia 23 7School for Viticulture and Enology, University of Nova Gorica, Nova Gorica, Slovenia 24 8Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic
    [Show full text]
  • Monitoring Methodology and Protocols for 20 Habitats, 20 Species and 20 Birds
    1 Finnish Environment Institute SYKE, Finland Monitoring methodology and protocols for 20 habitats, 20 species and 20 birds Twinning Project MK 13 IPA EN 02 17 Strengthening the capacities for effective implementation of the acquis in the field of nature protection Report D 3.1. - 1. 7.11.2019 Funded by the European Union The Ministry of Environment and Physical Planning, Department of Nature, Republic of North Macedonia Metsähallitus (Parks and Wildlife Finland), Finland The State Service for Protected Areas (SSPA), Lithuania 2 This project is funded by the European Union This document has been produced with the financial support of the European Union. Its contents are the sole responsibility of the Twinning Project MK 13 IPA EN 02 17 and and do not necessarily reflect the views of the European Union 3 Table of Contents 1. Introduction .......................................................................................................................................................... 6 Summary 6 Overview 8 Establishment of Natura 2000 network and the process of site selection .............................................................. 9 Preparation of reference lists for the species and habitats ..................................................................................... 9 Needs for data .......................................................................................................................................................... 9 Protocols for the monitoring of birds ....................................................................................................................
    [Show full text]
  • Evolutionary History of Fumitories (Subfamily Fumarioideae, Papaveraceae): an Old Story Shaped by the Main Geological and Climatic Events in the Northern Hemisphere Q
    Molecular Phylogenetics and Evolution 88 (2015) 75–92 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Evolutionary history of fumitories (subfamily Fumarioideae, Papaveraceae): An old story shaped by the main geological and climatic events in the Northern Hemisphere q Miguel A. Pérez-Gutiérrez a, Ana T. Romero-García a, M. Carmen Fernández b, G. Blanca a, ⇑ María J. Salinas-Bonillo c, Víctor N. Suárez-Santiago a, a Department of Botany, Faculty of Sciences, University of Granada, c/ Severo Ochoa s/n, 18071 Granada, Spain b Department of Cell Biology, Faculty of Sciences, University of Granada, c/ Severo Ochoa s/n, 18071 Granada, Spain c Department of Biology and Geology, University of Almería, c/ Carretera de Sacramento s/n, 04120 Almería, Spain article info abstract Article history: Fumitories (subfamily Fumarioideae, Papaveraceae) represent, by their wide mainly northern temperate Received 21 July 2014 distribution (also present in South Africa) a suitable plant group to use as a model system for studying Revised 30 March 2015 biogeographical links between floristic regions of the Northern Hemisphere and also the Southern Accepted 31 March 2015 Hemisphere Cape region. However, the phylogeny of the entire Fumarioideae subfamily is not totally Available online 7 April 2015 known. In this work, we infer a molecular phylogeny of Fumarioideae, which we use to interpret the bio- geographical patterns in the subfamily and to establish biogeographical links between floristic regions, Keywords: such as those suggested by its different inter- and intra-continental disjunctions. The tribe Hypecoeae Ancestral-area reconstruction is the sister group of tribe Fumarieae, this latter holding a basal grade of monotypic or few-species genera Biogeography Fumarioideae with bisymmetric flowers, and a core group, Core Fumarieae, of more specious rich genera with zygomor- Molecular dating phic flowers.
    [Show full text]
  • Recorder 14 (Spring 2010)
    BSBI Recorder www.bsbi.org.uk Spring 2010 Viscum album – our flagship species for the start of Date Class 5. Blue dots show records so far in 2010. BBSSBBII RReeccoorrddeerr NNoo.. 1144 A newsletter for county recorders, referees and herbarium curators in the Botanical Society of the British Isles Spring 2010 Contents Summary....................................................................3 Maps Scheme report...................................................4 Filling the gaps...........................................................6 Herbaria at Home.......................................................7 Using the BSBI Web Sites.........................................8 Plant Record notes .....................................................9 The status of Fumaria purpurea ..............................10 The status of Crepis mollis .......................................11 Meet the BSBI..........................................................16 County Roundup ......................................................19 Referees..........................................................27 Herbaria..........................................................27 © The Botanical Society of the British Isles, 2010 Botany Department, Natural History Museum, Cromwell Road, London, SW7 5BD Charity number 212560 in England & Wales; SC038675 in Scotland. www.bsbi.org.uk 1 Contacts Chair of Records Committee David Pearman, Algiers, Feock, Truro, Cornwall, TR3 6RA 01872 863388, [email protected] Head of Research & Development Kevin Walker, 97 Dragon
    [Show full text]
  • A Checklist of the Higher Plants of Vice-County Warwickshire
    A CHECKLIST OF THE HIGHER PLANTS OF VICE-COUNTY WARWICKSHIRE Notes to help you use the checklist The Checklist takes the form of a table, with columns for: scientific name, taken from Stace (2010) popular name, mostly taken from the same source category i.e. whether native, archaeophyte, neophyte, casual or extinct notes, giving an indication of frequency (including whether rare or very rare in Warwickshire), main habitat(s), localities for some of the rarities, whether the plants are annuals, biennial or perennial) rarity class i.e. whether a National Rarity, Warwickshire Rarity or Warwickshire Notable The sequence of the checklist is alphabetical by scientific name. Most of the terms used (e.g. archaeophyte, neophyte and hybrid) are explained in the introduction and the glossary, but two sets of terms used in the Checklist (Life expectancy and Frequency) are briefly explained here. Life expectancy An annual is a plant that lives for a maximum of one year; a biennial lives for two years (typically flowering in its second year), and a perennial lives for several or many years. Frequency The terms scarce, occasional, frequent, and abundant indicate increasing degrees of abundance. A local species is one that is very restricted in geographical terms, though it might be quite frequent where it occurs. An endemic is a species that only occurs in a particular place and nowhere else in the world: a hybrid water crowfoot and extinct hybrid pondweed have so far only been found in Warwickshire (so may be endemic to the County). The checklist is based upon Copson, Partridge & Roberts, 2008, and largely relies upon this work for decisions relating to a plant’s category (e.g.
    [Show full text]
  • Desktop Biodiversity Report
    Desktop Biodiversity Report Land at Aldingbourne Parish ESD/13/509 Prepared for Martin Beaton 27th September 2013 This report is not to be passed on to third parties without prior permission of the Sussex Biodiversity Record Centre. Please be aware that printing maps from this report requires an appropriate OS licence. Sussex Biodiversity Record Centre report regarding land at Aldingbourne Parish 27/09/2013 Prepared for Martin Beaton Aldingbourne Parish Council ESD/13/509 The following information is enclosed within this report: Maps Sussex Protected Species Register Sussex Bat Inventory Sussex Bird Inventory UK BAP Species Inventory Sussex Rare Species Inventory Sussex Invasive Alien Species Full Species List Environmental Survey Directory SNCI Ar01 - Fontwell Park Racecourse; Ar09 - Slindon Bottom. SSSI None Other Designations/Ownership Environmental Stewardship Agreement; National Park; National Trust Property; Notable Road Verge. Habitats Ancient tree; Ancient woodland; Chalk stream; Coastal and floodplain grazing marsh; Lowland calcareous grassland; Lowland meadow; Traditional orchard. Important information regarding this report It must not be assumed that this report contains the definitive species information for the site concerned. The species data held by the Sussex Biodiversity Record Centre (SxBRC) is collated from the biological recording community in Sussex. However, there are many areas of Sussex where the records held are limited, either spatially or taxonomically. A desktop biodiversity report from SxBRC will give the user a clear indication of what biological recording has taken place within the area of their enquiry. The information provided is a useful tool for making an assessment of the site, but should be used in conjunction with site visits and appropriate surveys before further judgements on the presence or absence of key species or habitats can be made.
    [Show full text]
  • 2016 Newsletter 17
    SOMERSET RARE PLANTS GROUP Recording all plants growing wild in Somerset, not just the rarities 2016 Newsletter Issue no. 17 Editor Liz McDonnell Introduction We welcome all our new members and hope that you will fully participate in our activities in the com- ing year. Visit www.somersetrareplantsgroup.org.uk to see the current year’s meetings programme, Somerset Rare Plant Register, Newsletter archive, information on SRPG recording in Somerset and much more. In 2016 we started the year by participating in the Botanical Society of Britain & Ireland (BSBI) New year Plant Hunt. This is now an annual event and is gaining popularity each year. We spent the al- lotted -3 hour period on the sand dunes, foreshore, road verges and hedgerows and recorded 65 spe- cies in flower. We had three indoor meetings and 19 field meetings, some of them jointly with other groups—including BSBI, the Wild Flower Society (WFS), Bristol Naturalists’ Society (BNS) and Somerset Archaeological and Natural History Society (SANHS). Most of our meetings this year were for general recording, as all our Somerset records will go to the BSBI Atlas 2020 recording scheme, but individuals were also recording and monitoring our rare species for the ongoing Somerset Rare Plants Register. An important meeting this year was the Dandelion Weekend, a joint BSBI/SRPG venture which resulted in a large number of new county records—see the Field meeting reports and Plant Records later in this newsletter. We held one identification workshop (on the Daisy family) which was very successful, where SRPG members were helped to separate their Hawkbits from their Hawksbeards, and the other yellow and white daisies in this large complex family.
    [Show full text]
  • Contribution to the Knowledge on the Flora of Mt. Luboten, Sharri Mts., Kosovo
    Thaiszia - J. Bot., Košice, 30 (2): 115-160, 2020 THAISZIA https://doi.org/10.33542/TJB2020-2-01 JOURNAL OF BOTANY Contribution to the knowledge on the flora of Mt. Luboten, Sharri Mts., Kosovo Naim Berisha1,2*, Renata Ćušterevska2, Fadil Millaku1,3, Mitko Kostadinovski2 & Vlado Matevski2,4 1 Faculty of Mathematics and Natural Sciences, University of Prishtina, Kosovo, [email protected] 2 Institute of Biology, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University in Skopje, North Macedonia 3 Faculty of Agribusiness, University “Haxhi Zeka”, Peja, Kosovo 4 Macedonian Academy of Sciences and Arts, Skopje, North Macedonia Berisha N., Ćušterevska R., Millaku F., Kostadinovski M. & Matevski V. (2020): Contribution to the knowledge on the flora of Mt. Luboten, Sharri Mts., Kosovo. – Thaiszia – J. Bot. 30 (2): 115-160. Abstract: With the aim of improving the floristic knowledge of Kosovo, here we present an inventory of the plant taxa recorded and collected between the March 2015 and September 2019, in the mountain massif of Luboten, Sharri Mts., SE Kosovo. Field surveys were conducted repeatedly for four years, on each vegetation season. With this work we aimed to provide detailed data concerning the vascular flora richness and distributional patterns. Floristic samples were studied in all representative habitats and sites, concerning climate, exposition, altitude and bedrock composition. This research led to the identification of a total 853 plant taxa of vascular plants, belonging to 354 genera and 93 families. Among these taxa, 82 are Balkan endemics and 53 are included into the Red Book of Vascular Flora of Kosovo.
    [Show full text]
  • The Flora Checklist
    THE FLORA CHECKLI ST OF NORTHAMPTONSHIRE AND THE SOKE OF PETERBOROUGH ROB WILSON 2014 1 A FLORA CHECKLIST OF NORTHAMPTONSHIRE & THE SOKE OF PETERBOROUGH : 2014 INTRODUCTION Neophyte The first edition of this checklist was published in 2008 as A naturalised plant believed to have been introduced into an aide to members of the Northamptonshire Flora Group the vice-county by man, after the year 1500 AD; it is who were recording the distribution of species for the considered to be naturalised if it maintains itself or forthcoming new Flora of Northamptonshire and the Soke increases year by year either by seed set or vegetative of Peterborough, due for publication in 2012 - 2013. It spread. was based on the species detailed in the 1995 Flora of Introduction Northamptonshire and the Soke of Peterborough. Recording for the projected new flora has brought to light An alien plant that has been deliberately introduced into a considerable number of species not previously found in the countryside or urban areas; included in this section the county. This has made it necessary to update the are trees that have been deliberately introduced and checklist. This edition contains all the species ever found which will survive for many years but will become extinct in the vice-county up to end of 2011. It is the intention to if not re-introduced. As a general rule trees in gardens keep this check-list updated and to post an updated have not been recorded although occasionally exceptions version on the BSBI web site at regular intervals. have been made for specimen trees of trees of some historic interest.
    [Show full text]
  • Vascular Flora of the Prometanj Site (Mokra Gora, Northern Prokletije Mt.)
    Зборник Матице српске за природне науке / Matica Srpska J. Nat. Sci. Novi Sad, № 130, 53—73, 2016 UDC 581.92 (497.11 Mokra gora) DOI: 10.2298/ZMSPN1630053R Boris Đ. RADAK1 * , Bojana S. BOKIĆ1 , Nusret F. PRELJEVIĆ2 , Milica M. RAT1 , Đurđica B. JANJIĆ1 , Jelena M. KNEŽEVIĆ1 , Goran T. ANAČKOV1 1 University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia 2 State University of Novi Pazar, Department of Biomedical Sciences, Vuka Karadžića b.b., 36300 Novi Pazar, Serbia VASCULAR FLORA OF THE PROMETANJ SITE (MOKRA GORA, NORTHERN PROKLETIJE MT.) ABSTRACT: Floristic research of the Prometanj site, located in the northwestern part of Mokra Gora Mt. along the right bank of the Ibar River, was conducted during 2011. A total of 340 species and five subspecies of vascular plant taxa were registered. Families with the larg- est number of species were Asteraceae, Fabaceae, Rosaceae, Lamiaceae, Ranunculaceae, while the most numerous genera were Trifolium, Acer, Campanula, Geranium, Veronica, Ranunculus and Vicia. Floral elements of analyzed plant taxa were grouped into ten areal types, with domination of Central European and Eurasian and significant participation of Mediterranean-Submediterranean. The biological spectrum was characterized by the dom- inance of hemicryptophytes. Five strictly protected and 43 protected species were registered. Prometanj is the only remaining locality in Serbia for tertiary species Adenophora liliifolia. Floristic research of Prometanj should be extended to entire area of Mokra Gora Mt. together with the Ibar River gorge, in order to explore the whole botanical richness of this area. KEYWORDS: arel types spectrum, biological spectrum, Prometanj, Prokletije, vas- cular flora INTRODUCTION Serbian part of the Prokletije mountain range is located in the extreme southwest of the country, on the tripoint with Montenegro and Albania.
    [Show full text]
  • Vascular Plants of Greece: an Annotated Checklist
    Vascular plants of Greece: An annotated checklist. Supplement Author(s): Panayotis Dimopoulos, Thomas Raus, Erwin Bergmeier, Theophanis Constantinidis, Gregoris Iatrou, Stella Kokkini, Arne Strid & Dimitrios Tzanoudakis Source: Willdenowia, 46(3):301-347. Published By: Botanic Garden and Botanical Museum Berlin (BGBM) https://doi.org/10.3372/wi.46.46303 URL: http://www.bioone.org/doi/full/10.3372/wi.46.46303 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. Willdenowia Annals of the Botanic Garden and Botanical Museum Berlin-Dahlem PANAYOTIS DIMOPOULOS1*, THOMAS RAUS2, ERWIN BERGMEIER3, THEOPHANIS CONSTANTINIDIS4, GREGORIS IATROU1, STELLA KOKKINI5, ARNE STRID6 & DIMITRIOS TZANOUDAKIS1 Vascular plants of Greece: An annotated checklist. Supplement Version of record first published online on 26 October 2016 ahead of inclusion in December 2016 issue. Abstract: Supplementary information on taxonomy, nomenclature, distribution within Greece, total range, life form and ecological traits of vascular plants known to occur in Greece is presented and the revised data are quantitatively analysed.
    [Show full text]
  • Assessing Sampling Coverage of Species Distribution in Biodiversity
    Assessing sampling coverage of species distribution in biodiversity databases Maria Sporbert, Helge Bruelheide, Gunnar Seidler, Petr Keil, Ute Jandt, Gunnar Austrheim, Idoia Biurrun, Juan Antonio Campos, Andraž Čarni, Milan Chytrý, et al. To cite this version: Maria Sporbert, Helge Bruelheide, Gunnar Seidler, Petr Keil, Ute Jandt, et al.. Assessing sampling coverage of species distribution in biodiversity databases. Journal of Vegetation Science, Wiley, 2019, 30 (4), pp.620-632. 10.1111/jvs.12763. hal-02357061 HAL Id: hal-02357061 https://hal.archives-ouvertes.fr/hal-02357061 Submitted on 13 Nov 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 1 Assessing sampling coverage of species distribution in biodiversity 2 databases 3 4 Running title: Sampling coverage by box-counting 5 6 Maria Sporbert 1,2 *, Helge Bruelheide 1,2, Gunnar Seidler 1, Petr Keil 1,3, Ute Jandt 1,2, Gunnar 7 Austrheim 4, Idoia Biurrun 5, Juan Antonio Campos 5, Andraž Čarni 6,7, Milan Chytrý 8, János Csiky 9, 8 Els De Bie 10, Jürgen Dengler 2,11,12, Valentin Golub 13, John-Arvid
    [Show full text]