1 1 Methods and Resource Article 2 3 Microsatellite discovery in an insular amphibian (Grandisonia 4 alternans) with comments on cross-species utility and the accuracy of 5 locus identification from unassembled Illumina data 6 7 Eleanor A. S. Adamson1 • Anwesha Saha1, 2, 3 • Simon T. Maddock1, 2, 4 • Ronald 5 1 1 8 A. Nussbaum • David J. Gower • Jeffrey W. Streicher 9 10 11 1Department of Life Sciences, The Natural History Museum, London SW7 5BD, UK 12 13 2Department of Genetics, Evolution and Environment, University College London, 14 London WC1E 6BT, UK 15 16 3Ashoka Trust for Research of Ecology and the Environment, Bangalore, India 17 18 4Department of Animal Management, Reaseheath College, Nantwich, CW5 6DF, UK. 19 20 5Department of Ecology and Evolutionary Biology, University of Michigan, Ann 21 Arbor, Michigan 48109, USA 22 23 _____________________________________________________________________ 24 Eleanor A. S. Adamson and Anwesha Saha have contributed equally to this work. 25 _____________________________________________________________________ 26 27 Corresponding Author: Jeffrey W. Streicher (
[email protected]) 28 29 Submitted to Conservation Genetics Resources 30 5 Figures, 2 Tables, 1 Table Online 31 Word counts: Abstract, 250 words [250 max]; Main text/references, 4,997 [6000 max] 32 33 2 34 35 Abstract The Seychelles archipelago is unique among isolated oceanic islands 36 because it features an endemic radiation of caecilian amphibians (Gymnophiona). In 37 order to develop population genetics resources for this system, we identified 38 microsatellite loci using unassembled Illumina MiSeq data generated from a genomic 39 library of Grandisonia alternans, a species that occurs on multiple islands in the 40 archipelago.