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HSV-1) Glycoprotein K (Gk) in the Morphogenesis of Infectious Virion Particles

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HSV-1) Glycoprotein K (Gk) in the Morphogenesis of Infectious Virion Particles Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1999 Molecular Genetics and Functions of Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein K (gK) in the Morphogenesis of Infectious Virion Particles. Timothy Paul Foster Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Foster, Timothy Paul, "Molecular Genetics and Functions of Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein K (gK) in the Morphogenesis of Infectious Virion Particles." (1999). LSU Historical Dissertations and Theses. 7082. https://digitalcommons.lsu.edu/gradschool_disstheses/7082 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor qualify illustrations and photographs, print bleedthrough. substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher qualify 6" x 9” black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. Bell & Howell Information and Learning 300 North Zeeb Road. Ann Arbor, Ml 48106-1346 USA 800-521-0600 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. MOLECULAR GENETICS AND FUNCTIONS OF HERPES SIMPLEX VIRUS TYPE 1 (HSV-1) GLYCOPROTEIN K (gK) IN THE MORPHOGENESIS OF INFECTIOUS VIRION PARTICLES A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor o f Philosophy in The Interdepartmental Program in Veterinary Medical Sciences through the Department of Veterinary Microbiology And Parasitology By Timothy P. Foster B.Sc. Louisiana State University, 1995 B.Sc. Louisiana State University, 1995 December, 1999 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. UMI Number 9960053 UMI* UMI Microform9960053 Copyright 2000 by Bell & Howell Information and Learning Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. Bell & Howelt Information and Learning Company 300 North Zeeb Road P.O. Box 1346 Ann Arbor, Ml 48106-1346 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. DEDICATION This work is in memory of my grandfather, Charles Gillis Carraway, whom despite quite debilitating circumstances, which took away both his mental and physical capacities, always displayed the qualities of patience and understanding. Although it is the latter years of his life that I most remember, those memories do not do justice to a man who throughout his life struggled to better himself and those around him. His faith in my endeavors and vigilance in asking how my research was progressing will never be forgotten. He is my inspiration and motivation to keep reaching for my dreams in the midst of any and all adversity. ii Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. ACKNOWLEDGEMENTS I would like to express my deepest gratitude to my advisor Dr. K. G. “Gus” Kousoulas for his support, mentorship, confidence, and most of all friendship. He has been my father, brother, confidant and friend throughout both the good and the difficult times. He has provided me not only a sound base to build my research efforts upon, but also the knowledge that it takes to acquire funding for those efforts. The state of the art facilities, equipment, reagents and experience that his laboratories provided, gave me the wherewithal to succeed in the face of adversity. His dedication to his students, his laboratory, and his resourcefulness in funding will certainly always be remembered and admired. All to often Dr. Kousoulas spent long nights away from his family and often took time out of his personal life to help advance my research efforts and graduate studies. For this reason, I must also acknowledge the patience and understanding of his wife, Effie Kousoulas and his daughter Elena Kousoulas. I am indebted to the efforts of my department head, Dr. Johannes Storz for his guidance throughout my studies in developing important leadership qualities and organizational skills. His attention to detail has helped me develop both as a researcher and as a person. I am also extremely grateful for his efforts in acquiring my graduate fellowship through the Louisiana Board of Regents Fellowship Program. I send my thanks to my committee members, Dr. Ding Shih, Dr. Kathy O’Reilly, Dr. Elmer Godeny, Dr. Roger Laine, and Dr. Fred Rainey. This is not only for their efforts during my proposal, general exam, and defense, but for their iii Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. efforts in the classroom as well. I have not known a more qualified and diligent group of teachers. Each has contributed their own unique style and interpretations to the way that I now perceive so many things in life and in science. Their efforts through courses in the Department of Biological Sciences and the Department of Veterinary Microbiology and Parasitology has provided me with a diverse and well rounded background in the biological sciences that will follow me in all my future endeavors. I would like to thank Dr. Roger Laine for his insight into the entrepreneurial workings of both science and academics. I have spent many hours with he and his family discussing science, business, and life as well as partaking in his incredible cooking. His efforts at taking basic science into real-world application must be applauded. I would like to give special thanks to all those in GeneLab that provided both personal and professional assistance, Dr. Vladimir Chouljenko, Dr. A. Baghian, Li Huang, Susan Newman, Rafael Luna, Sukhanaya Jayachandra, Jim Cavacoli, and Tamara Chouljenko. I have learned so much from each of you and your diverse cultures and thank you all for your patience and tolerance during some of my worse moments. To the most special person in my life during this sometimes trying ordeal, I would like Galena Rybachuk to know that I have the greatest love and admiration for her. Despite her seemingly gentle nature, you have been the rock that has always been there when I needed someone to lean upon. I know I leaned a bit too hard at times and for this I am sorry. I hope that in our future years together we will iv Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. be able to look on this time as not only productive and enjoyable, but as some o f the fondest memories that we will ever share. Last but certainly not least, I would like to especially thank my family. Despite their initial efforts to steer me in some (or any) other direction, my father, Mr. Edwin E. Foster Jr. and mother, Mrs. Cheryl A. Carraway Foster have been extremely supportive throughout these four years. We have certainly grown closer during this time than we ever have been in our lives. Your undying and unfettered support is greatly appreciated and acknowledged. I must especially thank you for all the sacrifices you have made along the way to make my dreams a reality. My time here would not have been as cherished without the continuous chiming in of my sister, Amy E. Foster. Her unique outlook on life and on me has been a driving force in my life. I have never known someone so opposite and yet so similar as her. I would like to wish her the best in her efforts to follow in my footsteps in attaining an advanced degree, but she is one that must and will blaze her own trails. v Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. TABLE OF CONTENTS DEDICATION ________________________________________________________ ii ACKNOWLEDGEMENTS____________________________________________ iii LIST OF TABLES____________________________________________________ r i LIST OF FIGURES__________________________________________________ xu ABSTRACT _________________________________________________________ xx CHAPTER I: INTRODUCTION -------------------------------------------------------------
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