A Hybrid of Buddleja Davidii Var. Nanhoensis 'Nanho Purple' and B. Lindleyana
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HORTSCIENCE 39(7):1581–1583. 2004. In total, 511 fl owers were self-pollinated on 11 infl orescences of the hybrid. In total, 774 fl owers on 11 infl orescences of B. davidii ‘Nanho A Hybrid of Buddleja davidii var. Purple’ were self-pollinated. Four panicles of B. lindleyana were self-pollinated, resulting in nanhoensis ‘Nanho Purple’ and a total of 189 fl owers. Fertility of the hybrid relative to both par- B. lindleyana ents was assessed by crossing all three taxa as male parents to B. fallowiana and to B. davidii W. Elliott1, D.J. Werner2 and P.R. Fantz2 cultivars ‘White Ball’, ‘Black Knight’, and Department of Horticultural Science, North Carolina State University, Box ‘Dartmoor’, using the hybrid and both parents 7609, Raleigh, NC 27695-7609 as the pollen source. Between 7 and 13 infl ores- cences were pollinated for each combination, Additional index words. Buddleja davidii, Buddleja lindleyana, butterfl y bush, Buddlejaceae, with total fl owers pollinated ranging from Loganiaceae, Scrophulariaceae, breeding, hybrids, RAPD analysis 430 to 1664 fl owers per combination. Seed capsules were harvested at maturity, as defi ned Abstract. A controlled cross between Buddleja davidii var. nanhoensis (Chitt.) Rehd. ‘Nanho by their dried appearance and the beginning of Purple’ and B. lindleyana Fort. ex Lindl. produced a hybrid. Pollen viability, male fertility, dehiscence. The seed capsules were placed in and the fl oral and vegetative characters are presented with a Latin diagnosis. Buddleja paper envelopes, dried for an additional 2 to 3 x luteolufaucia Elliott and Fantz is proposed as the name for this hybrid. Hybridity was d at room temperature, and stored at 4 °C until confi rmed using RAPD analysis. they were sown. For germination, seeds were Buddleja L. (orthogr. syn. Buddleia) consists (Dirr, 1998). A successful interspecifi c hybrid of sown on sterile media (Fafard # 4) and placed of ≈100 species (Griffi ths, 1994). The genus B. davidii var. nanhoensis (Chitt.) Rehd. ‘Nanho under intermittent mist. The number of germi- has been placed in several families including Purple’ and B. lindleyana has been made. The nated seedlings for each cross combination was Scrophulariaceae Juss. (Rogers, 1986) and Lo- objectives of this work described herein are to counted after 3 to 5 weeks. ganiaceae Mart. (Griffi ths, 1994) as well as its verify the hybrid status of the seedling produced, Pollen fertility of the hybrid and both parents own family Buddlejaceae K. Wilh. (Bhattacha- characterize the fl oral and vegetative characters was determined by culturing pollen obtained ryya and Johri, 1998). Most species are tropical of the hybrid, and to validate a name for the from newly opened fl owers on germination or subtropical shrubs originating in Asia, South hybrid taxon. media (Brewbaker and Kwack, 1963). A fi ne Africa, and North and South America. Buddleja camel’s hair brush was used to remove pollen has a number of qualities that make it a desir- Materials and Methods from the anthers of several fl owers representing able ornamental shrub. Plants exhibit extended several infl orescences of the same plant. Three fl owering periods and produce long panicles of Numerous controlled crosses between B. petri dishes containing media were made for fl owers that attract butterfl ies and bees. Buddleja davidii var. nanhoensis ‘Nanho Purple’ and each taxa. Pollen was placed on the media and are widely adaptable, tolerating poor soil, heat B. lindleyana were made, resulting in a single allowed to germinate for 3 h. Pollen germination and drought, and suffer from few pest problems, hybrid seedling (herein referred to as the hybrid). was measured by examining the pollen under the exceptions being nematodes (Dirr, 1998) ‘Nanho Purple’ was used as the female parent. 100× magnifi cation. Germination was defi ned and spider mites (Gillman, 1998). This seedling was grown in the N.C. State as the pollen tube being at least as long as the Garden use of Buddleja has been limited University Horticultural Science greenhouse in diameter of the pollen grain. This procedure was primarily to B. davidii Franch. However, there Raleigh, and stem cuttings were made of this replicated three times over the course of 5 weeks. are some notable exceptions. ‘Lochinch’ is an individual plant for further testing. Pollen fertility of the hybrid and both parents was offspring of hybridization between B. davidii In Spring 2001, three vegetatively propagated compared using a repeated measures ANOVA, and B. fallowiana Balf., and possesses the specimens each of the hybrid, B. davidii ‘Nanho with data composed of arcsine transformation silvery-gray foliage of B. fallowiana, and the Purple’ and B. lindleyana were transplanted of the percent pollen germination. hardiness, habit and panicle size of B. davidii. outdoors at the Horticultural Field Laboratory Randomly amplifi ed polymorphic DNA ‘Honeycomb’ and ‘Sungold’, both hybrids of B. in Raleigh and grown under standard cultural (RAPD) reactions were performed on leaf globosa Hope and B. davidii, have the yellow conditions. Characterization of floral and samples of the hybrid and each parent to confi rm fl ower color of B. globosa and foliar character vegetative characters of the hybrid and both hybridity. RAPD primers OPAV11, OPW-16, and plant habit intermediate between these two parents were made for two consecutive years. OPX-18, OPAA-11,(AC)8CA, (AC)8CT, species (Dirr, 1998). Winter leaf defoliation was assessed visually in (AC)8TT and (AG)8CC were used in accordance To date, hybridization of the Asian species B. January 2003. with previously reported procedures (Chaparro davidii and B. lindleyana Fort. ex Lindl. has not Color was assessed by observation using et al., 1994). been reported in literature. Buddleja lindleyana the Royal Horticultural Society Colour Chart Vouchers in fl ower of B. davidii ‘Nanho possesses characteristics that would be desirable (Royal Horticultural Society, 1986). Color Purple (6611 and 6613, NCSC; 6612, NA), to incorporate into B. davidii. It has evergreen to designations (e.g., reddish-brown) represent the B. lindleyana (6614 and 6616, NCSC; 6615, semi-evergreen foliage in zones 7 to 9, produces author’s personal perceptions, while the color NA) and the hybrid (6617 and 6619, NCSC; purple-colored panicles that are considerably value designations (e.g., RHS 174A) refer to 6618, NA) were collected by Fantz and Elliott. longer than B. davidii, and can be encouraged to corresponding RHS color chips. Vouchers sent to the United States National grow on a trellis or to weave through the canopy Self- and cross-fertility of the hybrid and Arboretum (NA) included 6612, 6615, and 6618, of a tree, where its long drooping panicles can be both parents were assessed by making controlled and the remaining vouchers were deposited at displayed with maximal effectiveness. Also, B. self- and cross-pollinations in the greenhouse. the NCSU Herbarium (NCSC). lindleyana is considered to be more mite resistant Cross-pollinations were made using standard hybridization procedures. For each pollination, Results and Discussion panicles containing fl owers one to two days Received for publication 21 Jan. 2004. Accepted before anthesis were selected. The fl owers were Morphological comparison of taxa. The for publication 27 Mar. 2004. The authors appreci- hybrid resembles both parents in having ate the contributions of W.E. Kloos for generating emasculated and fresh pollen of the male parent thought; L. Snelling, for support of this project; J. was applied with a camel’s hair brush to the angulate stems, opposite green leaves, purple- Chaparro for conducting the RAPD reactions; and stigmatic surface of each fl ower immediately violet corolla lobes, glabrous ovules, and four J. Dietz for statistical consultation. after emasculation. Pollinations were performed stamens. The infl orescence of each taxa is an 1Graduate student. over a four-month period of time, from July indeterminate thyrse. Fruits are capsules. A 2Professor. through November 2000. summary of distinguishing characters compar- HORTSCIENCE VOL. 39(7) DECEMBER 2004 1581 11581–1583COLOR.indd581–1583COLOR.indd 11581581 110/28/040/28/04 44:43:58:43:58 PPMM Table 1. A comparison of the hybrid characters with those of the parents Buddleja davidii ‘Nanho Purple’ and B. lindleyana. Characteristic B. davidii ‘Nanho Purple’ Hybrid B. lindleyana Stems Color older stem 165A-B 174A, 175A-B 172A, 174A Dark brown Reddish-brown Reddish-brown Suckering No No Yes Leaves Petiole length (mm) (0) 2–4 0–3 (5) (2) 3–6 (7) Length/width ratio 3.1–5.6 2.3–3.4 1.4–2.2 (2.6) Shape Lanceolate, oblong, oblanceolate, linear Lanceolate, oblong, elliptical Ovate-lanceolate Margin Serrate-crenate Serrate Entire (crenate) Lower surface color 194A-195A-196A 148C, 147B (194A) 147A, 147B, 147C Silver-white Pale green Pale green Lower surface pubescence White tomentose Pale tan on midrib of young leaves Brown pubescence on young leaves Upper surface Dull color Dull color Shiny Winter leaf defoliation 80% 15% 25% Infl orescence Rachis length (mm) 63–110 (125) (5) 85–212 (383) (132) 185–400 (450) Grouping 3, 5, 7 3 3 Flowers Flower length (mm) 13.1–16.9 (18.5) 16.4–21.8 (13.6) 14.3–18.7 Total number fl owers (39) 48–90 (26) 53–133 161–280 Number open fl owers (34) 41–90 3–27 (34) (1) 3–11 Odor Fragrant Nonfragrant Nonfragrant Calyx Width (mm) 0.9–1.6 1.3–2.2 1.8 – 2.3 Corolla Shape Salverform Salverform Tubular Straight Mostly straight, a few Curved curved Pubescence Sparse Dense Dense Lobe/tube width 4.1–7.6 3.0–5.6 1.6–2.8 Color–tube 80B-C 88D 84C, 85A Tube width (mm) 0.8–1.7 0.9–2.6 1.6–3.0 Lobe width (mm) (5.1) 5.5–8.4 (5.4) 6.1–9.1 4.1–6.1 Color–throat 25A (edge), 28A, 31A 158A, 20C 84C, 85C Orange-yellow Creamy yellow Purplish-white Stamen Length (mm) 12 12 7 Anther color 162A 166A 164A Muddy yellow Dark brown Medium red-brown Filament color 156B 166D,199B Clear, match throat Green-gray Taupe Seed capsules Length (mm) 6–12 6–12 4–6 Width (mm) (0.9) 1.3–2.1 1.9–2.9 (3.4) 2.2–3.6 Length/width 4.6–8.3 (10.8) (2.8) 3.6–4.7 (5.3) 1.1–2.4 ing the hybrid and both parents is presented in juvenile leaves, with less obvious brown pu- lindleyana produced viable seed after self-pol- (Table 1).