bioRxiv preprint doi: https://doi.org/10.1101/640623; this version posted May 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Root microbiota assembly and adaptive differentiation among European 2 Arabidopsis populations 3 4 Thorsten Thiergart1,7, Paloma Durán1,7, Thomas Ellis2, Ruben Garrido-Oter1,3, Eric Kemen4, Fabrice 5 Roux5, Carlos Alonso-Blanco6, Jon Ågren2,*, Paul Schulze-Lefert1,3,*, Stéphane Hacquard1,*. 6 7 1Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany 8 2Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, SE‐752 36 9 Uppsala, Sweden 10 3Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 11 50829 Cologne, Germany 12 4Department of Microbial Interactions, IMIT/ZMBP, University of Tübingen, 72076 Tübingen, 13 Germany 14 5LIPM, INRA, CNRS, Université de Toulouse, 31326 Castanet-Tolosan, France 15 6Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología (CNB), Consejo 16 Superior de Investigaciones Científicas (CSIC), 28049 Madrid, Spain 17 7These authors contributed equally: Thorsten Thiergart, Paloma Durán 18 *e-mail:
[email protected],
[email protected],
[email protected] 19 20 Summary 21 Factors that drive continental-scale variation in root microbiota and plant adaptation are poorly 22 understood. We monitored root-associated microbial communities in Arabidopsis thaliana and co- 23 occurring grasses at 17 European sites across three years.