Fine Spatial Scale Variation of Soil Microbial Communities Under European Beech and Norway Spruce
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Sphingomonas Sp. Cra20 Increases Plant Growth Rate and Alters Rhizosphere Microbial Community Structure of Arabidopsis Thaliana Under Drought Stress
fmicb-10-01221 June 4, 2019 Time: 15:3 # 1 ORIGINAL RESEARCH published: 05 June 2019 doi: 10.3389/fmicb.2019.01221 Sphingomonas sp. Cra20 Increases Plant Growth Rate and Alters Rhizosphere Microbial Community Structure of Arabidopsis thaliana Under Drought Stress Yang Luo1, Fang Wang1, Yaolong Huang1, Meng Zhou1, Jiangli Gao1, Taozhe Yan1, Hongmei Sheng1* and Lizhe An1,2* 1 Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China, 2 The College of Forestry, Beijing Forestry University, Beijing, China The rhizosphere is colonized by a mass of microbes, including bacteria capable of Edited by: promoting plant growth that carry out complex interactions. Here, by using a sterile Camille Eichelberger Granada, experimental system, we demonstrate that Sphingomonas sp. Cra20 promotes the University of Taquari Valley, Brazil growth of Arabidopsis thaliana by driving developmental plasticity in the roots, thus Reviewed by: Muhammad Saleem, stimulating the growth of lateral roots and root hairs. By investigating the growth Alabama State University, dynamics of A. thaliana in soil with different water-content, we demonstrate that Cra20 United States Andrew Gloss, increases the growth rate of plants, but does not change the time of reproductive The University of Chicago, transition under well-water condition. The results further show that the application of United States Cra20 changes the rhizosphere indigenous bacterial community, which may be due *Correspondence: to the change in root structure. Our findings provide new insights into the complex Hongmei Sheng [email protected] mechanisms of plant and bacterial interactions. The ability to promote the growth of Lizhe An plants under water-deficit can contribute to the development of sustainable agriculture. -
A Noval Investigation of Microbiome from Vermicomposting Liquid Produced by Thai Earthworm, Perionyx Sp
International Journal of Agricultural Technology 2021Vol. 17(4):1363-1372 Available online http://www.ijat-aatsea.com ISSN 2630-0192 (Online) A novel investigation of microbiome from vermicomposting liquid produced by Thai earthworm, Perionyx sp. 1 Kraisittipanit, R.1,2, Tancho, A.2,3, Aumtong, S.3 and Charerntantanakul, W.1* 1Program of Biotechnology, Faculty of Science, Maejo University, Chiang Mai, Thailand; 2Natural Farming Research and Development Center, Maejo University, Chiang Mai, Thailand; 3Faculty of Agricultural Production, Maejo University, Thailand. Kraisittipanit, R., Tancho, A., Aumtong, S. and Charerntantanakul, W. (2021). A noval investigation of microbiome from vermicomposting liquid produced by Thai earthworm, Perionyx sp. 1. International Journal of Agricultural Technology 17(4):1363-1372. Abstract The whole microbiota structure in vermicomposting liquid derived from Thai earthworm, Perionyx sp. 1 was estimated. It showed high richness microbial species and belongs to 127 species, separated in 3 fungal phyla (Ascomycota, Basidiomycota, Mucoromycota), 1 Actinomycetes and 16 bacterial phyla (Acidobacteria, Armatimonadetes, Bacteroidetes, Balneolaeota, Candidatus, Chloroflexi, Deinococcus, Fibrobacteres, Firmicutes, Gemmatimonadates, Ignavibacteriae, Nitrospirae, Planctomycetes, Proteobacteria, Tenericutes and Verrucomicrobia). The OTUs data analysis revealed the highest taxonomic abundant ratio in bacteria and fungi belong to Proteobacteria (70.20 %) and Ascomycota (5.96 %). The result confirmed that Perionyx sp. 1 -
Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure
Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure Authors: Suzanne L. Ishaq, Stephen P. Johnson, Zach J. Miller, Erik A. Lehnhoff, Sarah Olivo, Carl J. Yeoman, and Fabian D. Menalled The final publication is available at Springer via http://dx.doi.org/10.1007/s00248-016-0861-2. Ishaq, Suzanne L. , Stephen P. Johnson, Zach J. Miller, Erik A. Lehnhoff, Sarah Olivo, Carl J. Yeoman, and Fabian D. Menalled. "Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure." Microbial Ecology 73, no. 2 (February 2017): 417-434. DOI: 10.1007/s00248-016-0861-2. Made available through Montana State University’s ScholarWorks scholarworks.montana.edu Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure 1,2 & 2 & 3 & 4 & Suzanne L. Ishaq Stephen P. Johnson Zach J. Miller Erik A. Lehnhoff 1 1 2 Sarah Olivo & Carl J. Yeoman & Fabian D. Menalled 1 Department of Animal and Range Sciences, Montana State University, P.O. Box 172900, Bozeman, MT 59717, USA 2 Department of Land Resources and Environmental Sciences, Montana State University, P.O. Box 173120, Bozeman, MT 59717, USA 3 Western Agriculture Research Center, Montana State University, Bozeman, MT, USA 4 Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces, NM, USA Abstract Farming practices affect the soil microbial commu- then individual farm. Living inoculum-treated soil had greater nity, which in turn impacts crop growth and crop-weed inter- species richness and was more diverse than sterile inoculum- actions. -
Soil Ph Is the Primary Factor Driving the Distribution and Function of Microorganisms in Farmland Soils in Northeastern China
Annals of Microbiology (2019) 69:1461–1473 https://doi.org/10.1007/s13213-019-01529-9 ORIGINAL ARTICLE Soil pH is the primary factor driving the distribution and function of microorganisms in farmland soils in northeastern China Cheng-yu Wang1 & Xue Zhou1 & Dan Guo1 & Jiang-hua Zhao1 & Li Yan1 & Guo-zhong Feng1 & Qiang Gao1 & Han Yu2 & Lan-po Zhao1 Received: 26 May 2019 /Accepted: 3 November 2019 /Published online: 19 December 2019 # The Author(s) 2019 Abstract Purpose To understand which environmental factors influence the distribution and ecological functions of bacteria in agricultural soil. Method A broad range of farmland soils was sampled from 206 locations in Jilin province, China. We used 16S rRNA gene- based Illumina HiSeq sequencing to estimated soil bacterial community structure and functions. Result The dominant taxa in terms of abundance were found to be, Actinobacteria, Acidobacteria, Gemmatimonadetes, Chloroflexi, and Proteobacteria. Bacterial communities were dominantly affected by soil pH, whereas soil organic carbon did not have a significant influence on bacterial communities. Soil pH was significantly positively correlated with bacterial opera- tional taxonomic unit abundance and soil bacterial α-diversity (P<0.05) spatially rather than with soil nutrients. Bacterial functions were estimated using FAPROTAX, and the relative abundance of anaerobic and aerobic chemoheterotrophs, and nitrifying bacteria was 27.66%, 26.14%, and 6.87%, respectively, of the total bacterial community. Generally, the results indicate that soil pH is more important than nutrients in shaping bacterial communities in agricultural soils, including their ecological functions and biogeographic distribution. Keywords Agricultural soil . Soil bacterial community . Bacterial diversity . Bacterial biogeographic distribution . -
Cave Silver’ Biofilms on Rocks in the Former Homestake Mine in South Dakota, USA Amanpreet K
International Journal of Speleology 48 (2) 145-154 Tampa, FL (USA) May 2019 Available online at scholarcommons.usf.edu/ijs International Journal of Speleology Off icial Journal of Union Internationale de Spéléologie Culture-based analysis of ‘Cave Silver’ biofilms on Rocks in the former Homestake mine in South Dakota, USA Amanpreet K. Brar1 and David Bergmann2* 1Florida State University, 319 Stadium Dr., Tallahassee, FL 32306-4295, USA 2Black Hills State University, 1200 W. University St., Spearfish, SD 57789, USA Abstract: Tunnels in a warm, humid area of the 1478 m level of the Sanford Underground Research Facility (SURF), located in a former gold mine in South Dakota, USA, host irregular, thin whitish, iridescent biofilms, which appear superficially similar to ‘cave silver’ biofilms described from limestone and lava tube caves, despite the higher rock temperature (32°C) and differing rock surface (phyllite) present at SURF. In this study, we investigated the diversity of cultivable bacteria constituting the cave silver by using several media: CN agar, CN gellan gum and 0.1X R2A agar. The highest colony count (CFU/g of sample) was observed on 0.1X R2A medium. The bacterial strains were grouped into 39 distinct genotypes by randomly amplified polymorphic DNA (RAPD) analysis. In addition, the bacterial strains were further characterized based on their phenotypic and biochemical properties. 16S rRNA gene sequencing classified the cave silver isolates into three major bacterial phyla: Proteobacteria, Actinobacteria and Firmicutes. Isolates included some known genera; such as Taonella, Dongia, Mesorhizobium, Ralstonia, Pedomicrobium, Bauldia, Pseudolabrys, Reyrnella, Mizugakiibacter, Bradyrhizobium, Pseudomonas, Micrococcus, Sporichthya, Allokutzneria, Amycolatopsis, Pseudonocardia, and Paenibacillus. -
Genome-Informed Bradyrhizobium Taxonomy: Where to from Here?
Systematic and Applied Microbiology 42 (2019) 427–439 Contents lists available at ScienceDirect Systematic and Applied Microbiology jou rnal homepage: http://www.elsevier.com/locate/syapm Genome-informed Bradyrhizobium taxonomy: where to from here? a a a a,b Juanita R. Avontuur , Marike Palmer , Chrizelle W. Beukes , Wai Y. Chan , a c d e Martin P.A. Coetzee , Jochen Blom , Tomasz Stepkowski˛ , Nikos C. Kyrpides , e e f a Tanja Woyke , Nicole Shapiro , William B. Whitman , Stephanus N. Venter , a,∗ Emma T. Steenkamp a Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa b Biotechnology Platform, Agricultural Research Council Onderstepoort Veterinary Institute (ARC-OVI), Onderstepoort 0110, South Africa c Bioinformatics and Systems Biology, Justus-Liebig-University Giessen, Giessen, Germany d Autonomous Department of Microbial Biology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Poland e DOE Joint Genome Institute, Walnut Creek, CA, United States f Department of Microbiology, University of Georgia, Athens, GA, United States a r t i c l e i n f o a b s t r a c t Article history: Bradyrhizobium is thought to be the largest and most diverse rhizobial genus, but this is not reflected in Received 1 February 2019 the number of described species. Although it was one of the first rhizobial genera recognised, its taxon- Received in revised form 26 March 2019 omy remains complex. Various contemporary studies are showing that genome sequence information Accepted 26 March 2019 may simplify taxonomic decisions. Therefore, the growing availability of genomes for Bradyrhizobium will likely aid in the delineation and characterization of new species. -
Conserved and Reproducible Bacterial Communities Associate with Extraradical Hyphae of Arbuscular Mycorrhizal Fungi
The ISME Journal (2021) 15:2276–2288 https://doi.org/10.1038/s41396-021-00920-2 ARTICLE Conserved and reproducible bacterial communities associate with extraradical hyphae of arbuscular mycorrhizal fungi 1,2 1,3 1 Bryan D. Emmett ● Véronique Lévesque-Tremblay ● Maria J. Harrison Received: 21 September 2020 / Revised: 21 January 2021 / Accepted: 29 January 2021 / Published online: 1 March 2021 © The Author(s) 2021. This article is published with open access Abstract Extraradical hyphae (ERH) of arbuscular mycorrhizal fungi (AMF) extend from plant roots into the soil environment and interact with soil microbial communities. Evidence of positive and negative interactions between AMF and soil bacteria point to functionally important ERH-associated communities. To characterize communities associated with ERH and test controls on their establishment and composition, we utilized an in-growth core system containing a live soil–sand mixture that allowed manual extraction of ERH for 16S rRNA gene amplicon profiling. Across experiments and soils, consistent enrichment of members of the Betaproteobacteriales, Myxococcales, Fibrobacterales, Cytophagales, Chloroflexales, and Cellvibrionales was observed on ERH samples, while variation among samples from different soils was observed primarily 1234567890();,: 1234567890();,: at lower taxonomic ranks. The ERH-associated community was conserved between two fungal species assayed, Glomus versiforme and Rhizophagus irregularis, though R. irregularis exerted a stronger selection and showed greater enrichment for taxa in the Alphaproteobacteria and Gammaproteobacteria. A distinct community established within 14 days of hyphal access to the soil, while temporal patterns of establishment and turnover varied between taxonomic groups. Identification of a conserved ERH-associated community is consistent with the concept of an AMF microbiome and can aid the characterization of facilitative and antagonistic interactions influencing the plant-fungal symbiosis. -
Characterization of Axenic Immune Deficiency in Arabidopsis Thaliana
CHARACTERIZATION OF AXENIC IMMUNE DEFICIENCY IN ARABIDOPSIS THALIANA By James Michael Kremer A DISSERTATION Submitted to Michigan State University in partial fulfillment of the requirements for the degree of Microbiology and Molecular Genetics – Doctor of Philosophy 2017 ABSTRACT CHARACTERIZATION OF AXENIC IMMUNE DEFICIENCY IN ARABIDOPSIS THALIANA By James Michael Kremer Evolution of land plants began and has since occurred, in concert with complex communities of microorganisms, giving rise to a vast spectrum of plant-microbe relationships. Over the past decade, plant molecular biologists and microbial ecologists have worked together to identify drivers of microbiome composition that inspire hypotheses about microbiome functional potential, but many fall short of offering empirical evidence of microbiome-mediated influence on host phenotypes. Herein, I introduce a new suite of tools to explore microbiome function and report that many facets of plant immunocompetence are microbiome-dependent. Chapter One summarizes the current understanding of plant innate immunity and notable progress of plant microbiome research, including: (1) detection and response to microbe-associated molecular patterns, (2) hormone signaling during biotic interactions, (3) technology for exploration of plant microbiome ecology, (4) factors that influence microbiome community structure, and (5) a review of relevant model systems and gnotobiotic growth platforms. Chapter Two describes the development of a novel “FlowPot” growth system: a peat-based platform conducive -
Microbial Community Response to Environmental Changes in A
Microbial community response to environmental changes in a technosol historically contaminated by the burning of chemical ammunitions Hugues Thouin, Fabienne Battaglia-Brunet, Marie-Paule Norini, Catherine Joulian, Jennifer Hellal, Lydie Le Forestier, Sébastien Dupraz, Pascale Gautret To cite this version: Hugues Thouin, Fabienne Battaglia-Brunet, Marie-Paule Norini, Catherine Joulian, Jennifer Hellal, et al.. Microbial community response to environmental changes in a technosol historically contaminated by the burning of chemical ammunitions. Science of the Total Environment, Elsevier, 2019, 697, 134108 (11 p.). 10.1016/j.scitotenv.2019.134108. insu-02279732 HAL Id: insu-02279732 https://hal-insu.archives-ouvertes.fr/insu-02279732 Submitted on 10 Dec 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 1 Microbial community response to environmental changes in a technosol 2 historically contaminated by the burning of chemical ammunitions. 3 Hugues Thouin a,b Fabienne Battaglia-Brunet a,b, Marie-Paule Norini b, Catherine Joulian a, 4 Jennifer Hellal -
Successional Variation in the Soil Microbial Community in Odaesan National Park, Korea
sustainability Article Successional Variation in the Soil Microbial Community in Odaesan National Park, Korea Hanbyul Lee 1, Seung-Yoon Oh 2 , Young Min Lee 1, Yeongseon Jang 3, Seokyoon Jang 1, Changmu Kim 4, Young Woon Lim 5,* and Jae-Jin Kim 1,* 1 Division of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, Korea; [email protected] (H.L.); [email protected] (Y.M.L.); [email protected] (S.J.) 2 Department of Biology and Chemistry, Changwon National University, Changwon 51140, Korea; [email protected] 3 Division of Special Forest Production, National Institute of Forest Science, Seoul 02455, Korea; [email protected] 4 Microorganism Resources Division, National Institute of Biological Resources, Incheon 22689, Korea; [email protected] 5 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Korea * Correspondence: [email protected] (Y.W.L.); [email protected] (J.-J.K.); Tel.: +82-2-880-6708 (Y.W.L.); +82-2-3290-3049 (J.-J.K.); Fax: +82-2-871-5191 (Y.W.L.); +82-2-3290-9753 (J.-J.K.) Received: 4 May 2020; Accepted: 10 June 2020; Published: 11 June 2020 Abstract: Succession is defined as variation in ecological communities caused by environmental changes. Environmental succession can be caused by rapid environmental changes, but in many cases, it is slowly caused by climate change or constant low-intensity disturbances. Odaesan National Park is a well-preserved forest located in the Taebaek mountain range in South Korea. The forest in this national park is progressing from a mixed-wood forest to a broad-leaved forest. -
Impact of Agro-Farming Activities on Microbial Diversity of Acidic Red Soils in a Camellia Oleifera Forest
Rev Bras Cienc Solo 2019;43:e0190044 Article Division – Soil Processes and Properties | Commission – Soil Biology Impact of Agro-Farming Activities on Microbial Diversity of Acidic Red Soils in a Camellia Oleifera Forest Jun Li(1) , Zelong Wu(1) and Jun Yuan(1)* (1) Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China. ABSTRACT: The production of Camellia oleifera (oil tea), typically planted in acidic red soils in southern China, is limited by low soil fertility. Agro-farming is one way to promote soil fertility by increasing organic matter and microbial communities. To understand the impact of agro-farming activity on soil fertility, three types of agro-farming, namely, raising laying hens under forest (RLH), cultivating Lolium perenne grass under forest (LPG), and maintenance of native grass (MNG), were employed in an oil tea farm with acidic red soil in Changsha, China. Soil samples were collected from the farm to estimate microbial communities, pH, and total organic carbon (TOC) in different seasons. The results indicated that TOC and temperature were the dominant factors influencing the variations of bacterial communities, while temperature and pH affected the fungal communities in the soil. The most abundant bacterial phyla were Acidobacteria, Proteobacteria, Actinobacteria, and Chloroflexi, while the most abundant fungal phyla were Ascomycota, Basidiomycota, and Zygomycota. Regardless of treatment, the bacterial richness and diversity were both low in spring, and the fungal richness and diversity in summer and * Corresponding author: autumn were higher than in spring and winter. -
Supplemental Materials Oxidation of Ammonium by Feammox Acidimicr
Electronic Supplementary Material (ESI) for Environmental Science: Water Research & Technology. This journal is © The Royal Society of Chemistry 2019 Supplemental Materials Oxidation of Ammonium by Feammox Acidimicr:ae sp. A6 in Anaerobic Microbial Electrolysis Cells Melany Ruiz-Urigüen, Daniel Steingart, Peter R. Jaffé Corresponding author: P.R. Jaffé: [email protected] Reduction potential calculation for anaerobic ammonium oxidation to nitrite + The anaerobic ammonium (NH4 ) oxidation reaction that takes place in the absence of + - + iron oxides, in MECs is NH4 + 2H2O NO2 + 3H2 + 2H , where the anode functions as the electron acceptor. The reduction potential (△Eº) difference between two half reactions measured in volts (V) (△Eº = Eanode – Eº’substrate) determines the feasibility of such reaction. Therefore, to make the reaction feasible, Eanode needs to be above Eº’substrate which is equal to 0.07 V as shown in the calculations below based on equation S1. Eº’ = Eº’acceptor – Eº’donor (Eq. S1) Anodic half reaction in MEC Eº’ Reference - + - + NO3 + 10H + 8e ⇌ NH4 + 3H2O 0.36 V (Schwarzenbach et al. 2003) - + - - NO3 + 2H + 2e ⇌ NO2 + H2O 0.43 V (Schwarzenbach et al. 2003) - + - + NO2 + 8H + 6 e ⇌ NH4 + 2H2O - 0.07 V or + - + - NH4 + 2H2O ⇌ NO2 + 8H + 6 e 0.07 V Figure S1. Average current density measured in MECs with pure live A6 in Feammox medium + without NH4 under stirring conditions. Marks show the mean and lines the standard error (n=3). a. b. 0.8 1.2 0 mM ) 0.125 mM n 1 ) o 0.6 i t M 0.25 mM c 0.8 a m 0.5 mM r ( f ( - 0.4 1 mM 0.6 0 mM 2 ) I O I 0.125 mM ( 0.4 N 0.2 e 0.25 mM F 0.2 0.5 mM 1 mM 0 0 0 2 4 6 0 2 4 6 Time (days) Time (days) - - Figure S2.