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Redalyc.Comparación De Dos Métodos De Extracción De ADN A
Revista Colombiana de Biotecnología ISSN: 0123-3475 [email protected] Universidad Nacional de Colombia Colombia Cadavid Sánchez, Isabel Cristina; Rosero García, Doris Amanda; Uribe Soto, Sandra Inés Comparación de dos métodos de extracción de ADN a partir de plantas del género Solanum, subgénero Leptostemonum Revista Colombiana de Biotecnología, vol. XV, núm. 2, diciembre, 2013, pp. 186-192 Universidad Nacional de Colombia Bogotá, Colombia Disponible en: http://www.redalyc.org/articulo.oa?id=77629802021 Cómo citar el artículo Número completo Sistema de Información Científica Más información del artículo Red de Revistas Científicas de América Latina, el Caribe, España y Portugal Página de la revista en redalyc.org Proyecto académico sin fines de lucro, desarrollado bajo la iniciativa de acceso abierto ARTÍCULO CORTO Comparación de dos métodos de extracción de ADN a partir de plantas del género Solanum, subgénero Leptostemonum Comparison of two ADN extraction methods from plants belonging Solanum genus Leptostemonum subgenus. Isabel Cristina Cadavid Sánchez*, Doris Amanda Rosero García*, Sandra Inés Uribe Soto* Resumen Se evaluaron dos métodos para la extracción de ADN en plantas del género Solanum, con el fin de obtener ADN disponible y de buena calidad para la obtención de secuencias. El producto comercial DNeasy® Plant Mini Kit se comparó con un método que incluye el uso de una solución tampón de lisis. Para este último método también se evaluó si el rendimiento mejoraba cuando las muestras se maceraron previamente con nitrógeno líquido. Los resultados en términos de calidad (A260/A280) no mostraron diferencias significativas entre los métodos de extracción (índice < 1,5). Sin embargo, se encon- traron diferencias en la concentración de ADN obtenida (prueba de Dunnet, p<0,05) y en los porcentajes de amplificación mediante PCR (X2, p<0,05). -
Bulletin Biological Assessment Boletín RAP Evaluación Biológica
Rapid Assessment Program Programa de Evaluación Rápida Evaluación Biológica Rápida de Chawi Grande, Comunidad Huaylipaya, Zongo, La Paz, Bolivia RAP Bulletin A Rapid Biological Assessment of of Biological Chawi Grande, Comunidad Huaylipaya, Assessment Zongo, La Paz, Bolivia Boletín RAP de Evaluación Editores/Editors Biológica Claudia F. Cortez F., Trond H. Larsen, Eduardo Forno y Juan Carlos Ledezma 70 Conservación Internacional Museo Nacional de Historia Natural Gobierno Autónomo Municipal de La Paz Rapid Assessment Program Programa de Evaluación Rápida Evaluación Biológica Rápida de Chawi Grande, Comunidad Huaylipaya, Zongo, La Paz, Bolivia RAP Bulletin A Rapid Biological Assessment of of Biological Chawi Grande, Comunidad Huaylipaya, Assessment Zongo, La Paz, Bolivia Boletín RAP de Evaluación Editores/Editors Biológica Claudia F. Cortez F., Trond H. Larsen, Eduardo Forno y Juan Carlos Ledezma 70 Conservación Internacional Museo Nacional de Historia Natural Gobierno Autónomo Municipal de La Paz The RAP Bulletin of Biological Assessment is published by: Conservation International 2011 Crystal Drive, Suite 500 Arlington, VA USA 22202 Tel: +1 703-341-2400 www.conservation.org Cover Photos: Trond H. Larsen (Chironius scurrulus). Editors: Claudia F. Cortez F., Trond H. Larsen, Eduardo Forno y Juan Carlos Ledezma Design: Jaime Fernando Mercado Murillo Map: Juan Carlos Ledezma y Veronica Castillo ISBN 978-1-948495-00-4 ©2018 Conservation International All rights reserved. Conservation International is a private, non-proft organization exempt from federal income tax under section 501c(3) of the Internal Revenue Code. The designations of geographical entities in this publication, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of Conservation International or its supporting organizations concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. -
INSECTA: LEPIDOPTERA) DE GUATEMALA CON UNA RESEÑA HISTÓRICA Towards a Synthesis of the Papilionoidea (Insecta: Lepidoptera) from Guatemala with a Historical Sketch
ZOOLOGÍA-TAXONOMÍA www.unal.edu.co/icn/publicaciones/caldasia.htm Caldasia 31(2):407-440. 2009 HACIA UNA SÍNTESIS DE LOS PAPILIONOIDEA (INSECTA: LEPIDOPTERA) DE GUATEMALA CON UNA RESEÑA HISTÓRICA Towards a synthesis of the Papilionoidea (Insecta: Lepidoptera) from Guatemala with a historical sketch JOSÉ LUIS SALINAS-GUTIÉRREZ El Colegio de la Frontera Sur (ECOSUR). Unidad Chetumal. Av. Centenario km. 5.5, A. P. 424, C. P. 77900. Chetumal, Quintana Roo, México, México. [email protected] CLAUDIO MÉNDEZ Escuela de Biología, Universidad de San Carlos, Ciudad Universitaria, Campus Central USAC, Zona 12. Guatemala, Guatemala. [email protected] MERCEDES BARRIOS Centro de Estudios Conservacionistas (CECON), Universidad de San Carlos, Avenida La Reforma 0-53, Zona 10, Guatemala, Guatemala. [email protected] CARMEN POZO El Colegio de la Frontera Sur (ECOSUR). Unidad Chetumal. Av. Centenario km. 5.5, A. P. 424, C. P. 77900. Chetumal, Quintana Roo, México, México. [email protected] JORGE LLORENTE-BOUSQUETS Museo de Zoología, Facultad de Ciencias, UNAM. Apartado Postal 70-399, México D.F. 04510; México. [email protected]. Autor responsable. RESUMEN La riqueza biológica de Mesoamérica es enorme. Dentro de esta gran área geográfi ca se encuentran algunos de los ecosistemas más diversos del planeta (selvas tropicales), así como varios de los principales centros de endemismo en el mundo (bosques nublados). Países como Guatemala, en esta gran área biogeográfi ca, tiene grandes zonas de bosque húmedo tropical y bosque mesófi lo, por esta razón es muy importante para analizar la diversidad en la región. Lamentablemente, la fauna de mariposas de Guatemala es poco conocida y por lo tanto, es necesario llevar a cabo un estudio y análisis de la composición y la diversidad de las mariposas (Lepidoptera: Papilionoidea) en Guatemala. -
(Lepidoptera: Nymphalidae) En La Cuenca Del Río Coello, Colombia
Diversidad y distribución de mariposas Satyrinae (Lepidoptera: Nymphalidae) en la cuenca del río Coello, Colombia Jack F. García-Perez, Leonardo A. Ospina-López, Francisco A. Villa-Navarro & Gladys Reinoso-Flórez Grupo de Investigación en Zoología. Facultad de Ciencias. Universidad del Tolima (Colombia); Fax: (098) 2669182; [email protected], [email protected], [email protected], [email protected] Recibido 30-V-2004. Corregido 02-V-2006. Aceptado 15-I-2007. Abstract: Diversity and distribution of Satyrinae butterflies (Lepidoptera: Nymphalidae) in the Coello river basin, Colombia. We describe the patterns of diversity and distribution of the subfamily Satyrinae in the Coello river basin (4º 17’ 08’’ N - 74º 35’ 36’’ W; 1 899.31 km2) from 433 to 3 600 m. Eleven sampling stations were located, in several ecosystems. The samples were collected during a period of 11 days, in March, May, July and October 2003, in three types of habitat (forest, forest edge and meadow). We collected at random, between 09:00 and 15:00 hr, using entomological nets. A total of 239 individuals (13 genera, 34 species) were collected. The most abundant species were in the genus Pedaliodes (41.4 %). Richness and diversity had high values in mountainous zones and paramo (> 2 000 m). The greater diversity was measured in the forest edge. The similarity analysis produced two groups: species from warm (Euptychia hesione and Hermeuptychia hermes) and middle (Pseudohaetera hypaesia and Taygetis celia) climate zones; and species from mountain and paramo (Lymanopoda obsoleta, Pedaliodes polusca and Eretris calisto). Rev. Biol. Trop. 55 (2): 645-653. Epub 2007 June, 29. -
The Speciation History of Heliconius: Inferences from Multilocus DNA Sequence Data
The speciation history of Heliconius: inferences from multilocus DNA sequence data by Margarita Sofia Beltrán A thesis submitted for the degree of Doctor of Philosophy of the University of London September 2004 Department of Biology University College London 1 Abstract Heliconius butterflies, which contain many intermediate stages between local varieties, geographic races, and sympatric species, provide an excellent biological model to study evolution at the species boundary. Heliconius butterflies are warningly coloured and mimetic, and it has been shown that these traits can act as a form of reproductive isolation. I present a species-level phylogeny for this group based on 3834bp of mtDNA (COI, COII, 16S) and nuclear loci (Ef1α, dpp, ap, wg). Using these data I test the geographic mode of speciation in Heliconius and whether mimicry could drive speciation. I found little evidence for allopatric speciation. There are frequent shifts in colour pattern within and between sister species which have a positive and significant correlation with species diversity; this suggests that speciation is facilitated by the evolution of novel mimetic patterns. My data is also consistent with the idea that two major innovations in Heliconius, adult pollen feeding and pupal-mating, each evolved only once. By comparing gene genealogies from mtDNA and introns from nuclear Tpi and Mpi genes, I investigate recent speciation in two sister species pairs, H. erato/H. himera and H. melpomene/H. cydno. There is highly significant discordance between genealogies of the three loci, which suggests recent speciation with ongoing gene flow. Finally, I explore the phylogenetic relationships between races of H. melpomene using an AFLP band tightly linked to the Yb colour pattern locus (which determines the yellow bar in the hindwing). -
Combining Taxonomic and Functional Approaches to Unravel the Spatial Distribution of an Amazonian Butterfly Community
Environmental Entomology Advance Access published December 7, 2015 Environmental Entomology, 2015, 1–9 doi: 10.1093/ee/nvv183 Community and Ecosystem Ecology Research article Combining Taxonomic and Functional Approaches to Unravel the Spatial Distribution of an Amazonian Butterfly Community Marlon B. Grac¸a,1,2,3 Jose´W. Morais,1 Elizabeth Franklin,1,2 Pedro A. C. L. Pequeno,1,2 Jorge L. P. Souza,1,2 and Anderson Saldanha Bueno,1,4 1Biodiversity Coordination, National Institute for Amazonian Research, INPA, Manaus, Brazil ([email protected]; [email protected]; [email protected]; [email protected]; [email protected]; [email protected]), 2Center for Integrated Studies of Amazonian Biodiversity, CENBAM, Manaus, Brazil, 3Corresponding author, e-mail: marlon_lgp@hotmail. com, and 4Campus Ju´lio de Castilhos, Farroupilha Federal Institute of Education, Science and Technology, Brazil ([email protected]) Received 24 August 2015; Accepted 10 November 2015 Abstract This study investigated the spatial distribution of an Amazonian fruit-feeding butterfly assemblage by linking spe- cies taxonomic and functional approaches. We hypothesized that: 1) vegetation richness (i.e., resources) and abun- dance of insectivorous birds (i.e., predators) should drive changes in butterfly taxonomic composition, 2) larval diet breadth should decrease with increase of plant species richness, 3) small-sized adults should be favored by higher abundance of birds, and 4) communities with eyespot markings should be able to exploit areas with higher predation pressure. Fruit-feeding butterflies were sampled with bait traps and insect nets across 25 km2 of an Amazonian ombrophilous forest in Brazil. We measured larval diet breadth, adult body size, and wing marking of all butterflies. -
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QL 541 .1866 ENT The Journal of Research Lepidoptera Volume 46 2013 ISSN 0022 4324 (PRINT) 2156 5457 (ONLINE) THE LEPIDOPTERA RESEARCH FOUNDATION The Journal of Research on the Lepidoptera www.lepidopteraresearchfoundation.org ISSN 0022 4324 (print) 2156 5457 (online) Published by: The Lepidoptera Research Foundation, Inc. 9620 Heather Road Beverly Hills, California 90210-1757 TEL (310) 274 1052 E-mail: Editorial: [email protected] Technical: [email protected] Founder: William Hovanitz (1915-1977) Editorial Staff: Konrad Fiedler, University of Vienna, Editor [email protected] Nancy R. Vannucci, info manager [email protected] Associate Editors: Annette Aiello, Smithsonian Institution [email protected] Joaquin Baixeras, Universitat de Valencia [email protected] Marcelo Duarte, Universidade de Sao Paulo [email protected] Klaus Fischer, University of Greifswald [email protected] Krushnamegh Kunte, Natl. Center for Biol. Sci, India [email protected] Gerardo Lamas, Universidad Mayor de San Marcos [email protected]. pe Rudi Mattoni [email protected] Soren Nylin, Stockholm University [email protected] Naomi Pierce, Harvard University [email protected] Robert Robbins, Smithsonian Institution [email protected] Daniel Rubinoff, University of Hawaii [email protected] Josef Settele, Helmholtz Cntr. for Environ. Research-UFZ [email protected] Arthur M. Shapiro, University of California - Davis [email protected] Felix Sperling, University of Alberta [email protected] Niklas Wahlberg, University of Turku [email protected] Shen Horn Yen, National Sun Yat-Sen University [email protected] Manuscripts and notices material must be sent to the editor, Konrad Fiedler [email protected]. -
Castniidae of the Museum of Natural History of the University of Wrocław: New Findings from Friedrich Wilhelm Niepelt's Co
Nota Lepi. 44 2021: 123–132 | DOI 10.3897/nl.44.60261 Research Article Castniidae of the Museum of Natural History of the University of Wrocław: new findings from Friedrich Wilhelm Niepelt’s collection with comments on Karl Adolf Georg Lauterbach and August Weberbauer Jorge M. González1, Paweł J. Domagała2 1 Austin Achieve Public Schools, Austin, TX 78723, (Research Associate, McGuire Center for Lepidoptera and Biodiversity), USA; e-mail: [email protected] 2 University of Opole, Institute of Biology, ul. Oleska 22, 45-052 Opole, Poland; e-mail: [email protected] http://zoobank.org/14CA338A-3748-493B-9EB5-45C6583B0BEF Received 1 November 2020; accepted 8 March 2021; published: 23 April 2021 Subject Editor: Alberto Zilli. Abstract. Further results of our research into the Giant Butterfly-Moths (Castniidae) of the Museum of Nat- ural History (University of Wrocław) are presented. Castniids of the Niepelt collection had previously been reviewed. However, while curating other sections of the Lepidoptera collection, we discovered 18 misplaced specimens belonging to nine taxa of Castniidae, several of them bearing typical labels by Niepelt. Among them, two are of particular interest, insofar as they are associated with the world-class botanists August We- berbauer (1871–1948) and Karl Adolf Georg Lauterbach (1864–1937). Introduction Examination of rich collections of Castniidae and other insect groups in several Polish museums (González et al. 2013a, b; Domagała et al. 2015, 2017a, b; Domagała and Dobosz 2019) led the authors to continue the exploration of such ever-surprising depositories. The role of museums as important repositories of biodiversity has been stressed on many occasions (e.g., Burrell et al. -
Revised Species Definitions and Nomenclature of the Rose Colored Cithaerias Butterflies (Lepidoptera, Nymphalidae, Satyrinae)
Zootaxa 3873 (5): 541–559 ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ Article ZOOTAXA Copyright © 2014 Magnolia Press ISSN 1175-5334 (online edition) http://dx.doi.org/10.11646/zootaxa.3873.5.5 http://zoobank.org/urn:lsid:zoobank.org:pub:05BD334C-493D-4688-92E8-602943ECF57D Revised species definitions and nomenclature of the rose colored Cithaerias butterflies (Lepidoptera, Nymphalidae, Satyrinae) CARLA M. PENZ1, LAURA G. ALEXANDER2 & PHILIP J. DEVRIES3 Department of Biological Sciences, University of New Orleans, 2000 Lakeshore Dr. New Orleans, LA 70148, USA. E-mail: [email protected]; [email protected]; [email protected] Abstract This study provides updated species definitions for five rose-colored Cithaerias butterflies, starting with a historical over- view of their taxonomy. Given their mostly transparent wings, genitalia morphology yielded the most reliable characters for species definition and identification. Genitalic divergence is more pronounced when multiple species occur in sympa- try than between parapatric taxa. Cithaerias aurorina is granted full species status, C. cliftoni is reinstated as a full species, and one new combination is proposed, i.e. C. aurora tambopata. Two new synonyms are proposed, Callitaera phantoma and Callitaera aura = Cithaerias aurora. Key words: pireta, menander, aurorina, cliftoni, aurora, aura, phantoma, pyritosa Introduction Some of the most visually striking Neotropical butterflies belong to the genus Cithaerias Hübner (Satyrinae, Haeterini), which inhabit sea level to mid-elevation rainforests from Mexico through Central and South America. A characteristic of all Cithaerias species is their mostly transparent wings with the distal portions of the hind wing overlaid with partially lustrous rose, purple or blue scales. -
Phylogeny of Neotropical Castniinae (Lepidoptera: Cossoidea: Castniidae)
bs_bs_banner Zoological Journal of the Linnean Society, 2014, 170, 362–399. With 143 figures Phylogeny of Neotropical Castniinae (Lepidoptera: Cossoidea: Castniidae): testing the hypothesis of the mimics as a monophyletic group and implications for the arrangement of the genera SIMEÃO DE SOUZA MORAES1,2* and MARCELO DUARTE2 1Curso de Pós-Graduação em Ciências Biológicas (Zoologia), Instituto de Biociências, Departamento de Zoologia, Universidade de São Paulo, Rua do Matão, travessa 14, número 321, CEP 05508-900, São Paulo, São Paulo, Brazil 2Museu de Zoologia da Universidade de São Paulo, Avenida Nazaré 481, 04263-000, São Paulo, São Paulo, Brazil Received 19 March 2013; revised 11 October 2013; accepted for publication 13 October 2013 A cladistic analysis of the Neotropical Castniidae is presented using 120 morphological characters, and a taxonomic treatment based on that analysis is also presented. The tribe Gazerini as previously delimited was found to be paraphyletic with respect to the genera Ceretes, Divana, Riechia, Frostetola, and Oiticicastnia. The genera Castnia, Geyeria, and Athis were also found to be non-monophyletic taxa. The mimicry pattern had multiple independent origins in the Neotropical castniids, and at least two lineages, Riechia and Prometheus, are involved in Batesian mimicry rings with unpalatable butterfly models in the tribes Acraeini and Heliconiini (Nymphalidae). We propose for Castniini 13 new synonymies and 27 new combinations. Geyeria strigata (Walker, 1854) is revalidated. The generic placements of Athis superba (Strand, 1912) and Castnia eudesmia Gray, 1838 are questionable, but presently upheld. © 2014 The Linnean Society of London, Zoological Journal of the Linnean Society, 2014, 170, 362–399. doi: 10.1111/zoj.12102 ADDITIONAL KEYWORDS: Batesian mimicry – new synonyms – taxonomy. -
Hyvästit, Potkut Vaiko Lobbauksen Hedelmä? : Yhdysvaltain
B 128 OULU 2015 B 128 UNIVERSITY OF OULU P.O. Box 8000 FI-90014 UNIVERSITY OF OULU FINLAND ACTA UNIVERSITATIS OULUENSIS ACTA UNIVERSITATIS OULUENSIS ACTA HUMANIORAB Ari Uusitalo Ari Uusitalo Professor Esa Hohtola HYVÄSTIT, POTKUT VAIKO University Lecturer Santeri Palviainen LOBBAUKSEN HEDELMÄ? Postdoctoral research fellow Sanna Taskila YHDYSVALTAIN SUHTAUTUMINEN FILIPPIINIEN ITSENÄISYYSKYSYMYKSEEN JA SEN RATKAISUUN 1929–1934 Professor Olli Vuolteenaho University Lecturer Veli-Matti Ulvinen Director Sinikka Eskelinen Professor Jari Juga University Lecturer Anu Soikkeli Professor Olli Vuolteenaho OULUN YLIOPISTON TUTKIJAKOULU; OULUN YLIOPISTO, HUMANISTINEN TIEDEKUNTA, HISTORIA Publications Editor Kirsti Nurkkala ISBN 978-952-62-0822-0 (Paperback) ISBN 978-952-62-0823-7 (PDF) ISSN 0355-3205 (Print) ISSN 1796-2218 (Online) ACTA UNIVERSITATIS OULUENSIS B Humaniora 128 ARI UUSITALO HYVÄSTIT, POTKUT VAIKO LOBBAUKSEN HEDELMÄ? Yhdysvaltain suhtautuminen Filippiinien itsenäisyyskysymykseen ja sen ratkaisuun 1929–1934 Esitetään Oulun yliopiston ihmistieteiden tohtorikoulutus- toimikunnan suostumuksella julkisesti tarkastettavaksi Linnanmaan Kuusamonsalissa (YB210) 4. kesäkuuta 2015 klo 12.00 OULUN YLIOPISTO, OULU 2015 Copyright © 2015 Acta Univ. Oul. B 128, 2015 Työn ohjaajat Professori Kari Alenius Professori Olavi K. Fält Esitarkastajat Tohtori Tuula Okkonen Dosentti Markku Ruotsila Vastaväittäjä Tohtori Juhana Aunesluoma ISBN 978-952-62-0822-0 (Paperback) ISBN 978-952-62-0823-7 (PDF) ISSN 0355-3205 (Printed) ISSN 1796-2218 (Online) Kannen suunnittelu Raimo Ahonen JUVENES PRINT TAMPERE 2015 Uusitalo, Ari, Goodbye, lay-off of fruit of lobbying? The United States’ attitude towards the question of the independence of the Philippines and its solution in 1929–1934 University of Oulu Graduate School; University of Oulu, Faculty of Humanities, History Acta Univ. Oul. B 128, 2015 University of Oulu, P.O. -
Peru Conservation Recorded Wildlife at Taricaya
Peru Conservation Recorded Wildlife at Taricaya Butterflies (Mariposas) in Taricaya Reserve, Madre de Dios CLASS: Insecta ORDER: Lepidoptera 1. Familia Nymphalidae Subfamilia Apaturinae Doxocopa kallina (Staudinger, 1886). Doxocopa laure (Drury, 1776). Doxocopa lavinia (Butler, 1886). Doxocopa linda (C. Felder & R. Felder, 1860). Doxocopa pavon (Latreille, 1809). Subfamilia Nymphalinae Tribu Coeini Baetus aelius (Stoll, 1780). Baetus deucaliom (C. Felder & R. Felder, 1860). Baetus japetus (Staudinger, 1885). Colobura annulata (Willmot, Constantino & J. Hall, 2001). Colobura dirce (Linnaeus, 1758). Historis acheronta (Fabricius, 1775). Historis odius (Fabricius, 1775). Smyrna blomfilda (Fabricius, 1781). Tigridia acesta (Linnaeus, 1758). Tribu Kallimini Anartia jatrophae (Linnaeus, 1763). Junonia everate (Cramer, 1779). Junonia genoveva (Cramer, 1780). Metamorpha elissa (Hübner, 1818). Siproeta stelenes (Linnaeus, 1758). Tribu Melitaeini Eresia clio (Linnaeus, 1758). Eresia eunice (Hübner, 1807). Eresia nauplios (Linnaeus, 1758). Tegosa claudina (Escholtz, 1821). Tegosa fragilis (H. W. Bates, 1864). Tribu Nymphalini Hypanarthia lethe (Fabricius, 1793). Tribu Acraeini Actinote pellenea (Hübner, 1821). Subfamilia Charaxinae Tribu Preponini Agrias amydon (Hewitson, 1854). Agrias claudina (Godart, 1824). Archaeoprepona amphimacus (Fabricius, 1775). Archaeoprepona demophon (Linnaeus, 1758). Archaeoprepona meander (Cramer, 1775). Prepona dexamenus (Hopffer, 1874). Prepona laertes (Hübner, 1811). Prepona pheridamas (Cramer, 1777). Prepona pylene