Journal of Bacteriology

JOURNAL OF BACTERIOLOGY

VOLUME 126 * NUMBER 3 * JUNE 1976 EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1980) University of Delaware, Newark

S. G. BRADLEY, Editor (1979) ALLEN T. PHILLIPS, Editor (1980) Virginia Commonwealth University, Richmond Pennsylvania State University, University Park, Pa. ROY CURTISS HI, Editor (1979) CARL SCHNAITMAN, Editor (1978) University ofAlabama, Birmingham University of Virginia, Charlottesville ELIZABETH McFALL, Editor (1980) H. E. UMBARGER, Editor (1976) New York University, New York, N.Y. Purdue University, Lafayette, Ind.

James N. Adams (1976) Walton L. Fangman (1978) Eugene W. Nester (1978) Arthur I. Aronson (1976) Gerald R. Fink (1978) Donald P. Nierlich (1977) Gad Avigad (1977) W. R. Finnerty (1976) Michio Oishi (1977) Stephen D. Barbour (1976) Walter R. Guild (1978) Ronald H. Olsen (1978) Blaine Beaman (1976) George Hegeman (1977) Charles Panos (1978) Claire M. Berg (1976) James A. Hoch (1977) Leo Parks (1976) Richard S. Berk (1977) Joy Hochstadt (1977) Martin Pato (1978) Harriet Bernheimer (1977) Stanley C. Holt (1977) Paul V. Phibbs (1976) Judith S. Bond (1977) Joseph Inselburg (1978) Charles C. Remsen III (1976) Winfried Boos (1976) Sam Kaplan (1977) William S. Reznikoff (1976) Jean Brenchley (1977) Jost Kemper (1978) Palmer Rogers (1978) George H. Brownell (1977) David E. Kenneil (1977) Antonio H. Romano (1976) Richard 0. Burns (1977) Bruce C. Kline (1977) Robert Rownd (1977) Donald B. Clewell (1977) Robert L. Lester (1978) Harold L. Sadoff (1977) Stanley N. Cohen (1976) Hillel S. Levinson (1978) Irwin H. Segel (1976) Ronald S. Cole (1977) James S. Lovett (1977) Jane K. Setlow (1978) S. F. Conti (1978) K. Brooks Low (1976) Stanley K. Shapiro (1976) Stephen Cooper (1978) Calvin S. McLaughlin (1978) Simon Silver (1976) John E. Cronan, Jr. (1977) P. T. Magee (1977) Lucile Smith (1978) Walter J. Dobrogosz (1976) Michael H. Malamy (1978) James A. Wechsler (1978) Patrick R. Dugan (1977) Manley Mandel (1978) David White (1977) Alan D. Elbein (1976) Robert E. Marquis (1976) Clifford A. Woolfolk (1976) Wolfgang Epstein (1978) Neil H. Mendelson (1978) Howard Zalkin (1976) David P. Fan (1978) Gene E. Michaels (1977)

Robert A. Day, Managing Editor GiseUa Pollock, Director of Editorial Services Linda Illig, Production Editor 1913 I St., N. W., Washington, D. C. 20006 EX OFFICIO Helen R. Whiteley, President (1975-1976) Harlyn 0. Halvorson, Vice-President (1975-1976) J. Mehsen Joseph, Secretary Brinton M. Miller, Treasurer

The Journal ofBacteriology, a publication of the American submitted manuscripts, and general editorial matters should Society for Microbiology. 1913 I St., N.W., Washington, D.C. be directed to the ASM Publications Office, 1913 I St., N.W., 20006, is devoted to the advancement and dissemination of Washington, D.C. 20006 (area 202 833-9680). fundamental knowledge concerning bacteria and other microorganisms. The Journal is published monthly, and the twelve numbers are divided into four volumes per year. The non- Second class postage paid at Washington, D.C. 20006, and at member subscription price is $90 per year. Single copies are additional mailing offices. $8. The member subscription price is $17 per year. Corre- Made in the United States of America. spondence relating to subscriptions, reprints, defective copies, Copyright © 1976, American Society for Microbiology. availability of back issues, lost or late proofs, disposition of All Rights Reserved. Author Index Adams, James N., 1104 Hacking, Andrew J., 1166 Rake, Adrian V., 1305 Adhya, Sankar, 1108 Hamilton, W. Allan, 1224, 1232 Raymond, Jonathan, 1089 Hancock, Robert E. W., 1347 Rebouche, Charles J., 1207 Bass, Richard, 1119 Harriman, Philip D., 1141 Reeves, Peter, 1052, 1347 Beck, Barbara, 1250 Hebeler, Bruce H., 1180, 1186 Reyes, Oscar, 1108 Bergquist, P. L., 1063 Heffernan, Laurel, 1119, 1132 Rudd, Joanne, 1344 Bird, Robert E., 1215 Hochhauser, Steven J., 1082 Body, Barbara A., 1149 Holden, Joseph A., 1141 Sadaie, Yoshito, 1037 Broquist, Harry P., 1207 Sanemori, Hiroshi, 1030 Brownell, George H.,,1104 Ingebretsen, Ole C., 1075 Sanner, Tore, 1075 Brownstein, Bernard H., 1149 Ives, David H., 1136 Schaeffer, Pierre, 1342 Seto, Hiroyuki, 1113 Caro, Lucien, 1215 Jarvis, Audrey W., 1232 Shapiro, Lucille, 1305 Chandler, Michael, 1215 Shida, Hisatoshi, 1025 Chapman, Ann, 1351 Kadota, Hajime, 1342 Shinozawa, Takao, 1025 Cintr6n, Nitza M., 1082 Kapoor, V., 1245 Silbert, David F., 1351 Cole, Roger M., 1285 Kawasaki, Takashi, 1030 Slater, Martin L., 1339 Collins, Mary Lynne Perille, Kido, Toshiko, 1261 Soda, Kenji, 1261 1316, 1326 Kung, Fang-Chien, 1089 Subrahmanyam, C. S., 1042 Collins, Stephen H., 1224, 1232 Sypherd, Paul S., 1266 Curtiss, Roy, III, 1194 Lampky, J. R., 1278 Lin, E. C. C., 1166 Das, H. K., 1042 Lindsay, Raymond J., 1232 Tabita, F. Robert, 1271 Davies, John K., 1347 Lusk, Joan E., 1096 Tomasz, Alexander, 1113 Davis, Minh-Tam B., 1136 Truman, Penelope, 1063 McFadden, Bruce A., 1271 Egi, Yoshiko, 1030 Mendelson, Neil H., 1285 Uchida, Aritsune, 1342 Eisenberg, Roselyn J., 1344 Mooney, Duane T., 1266 Elchisak, Mary, 1344 Wall, Judy D., 1141 Englesberg, Ellis, 1119 Narui, Kikuko, 1037 Weiss, Bernard, 1082 Newman, E. B., 1245 Weiss, Richard L., 1173 Falkinham, Joseph O., III, 1194 Niederman, Robert A., 1316, White, Brian J., 1082 Foster, Gayle C., 1297 1326 Wilcox, Gary, 1132 Foster, R. Scott 1297 Wilson, David B., 1156 Park, James T., 1250 Wong, Brenda B., 1096 Glaser, Donald A., 1089 Park, Myung Hee, 1096 Wood, Nancy B., 1305 Gottesman, Max, 1108 Pohlman, Timothy, 1351 Potter, R., 1245 Yamamoto, Tatsuo, 1261 Haag, Steven M., 1285 Pugsley, Anthony P., 1052 Young, Frank E., 1180, 1186 INSTRUCTIONS TO AUTHORS Submit manuscripts in duplicate (original and Abstract. An Abstract appears at the beginning of one copy) to ASM Publications Office, 1913 I St., each paper in the Journal. The Abstract should not N.W., Washington, D.C. 20006. exceed 250 words. General policy. Any manuscript submitted must Literature Cited. In the text, references are cited be a report of unpublished original research, which by number. The Literature Cited section should be is not being considered for publication elsewhere. typed in alphabetical order, by first author, and Each manuscript should present the results of numbered. Each reference should include the title of an independent, cohesive study; numbered series the article and inclusive pagination. Names of papers are discouraged. Papers that deal primarily journals are abbreviated according to Bibliographic with a method should not be submitted. Guide for Editors & Authors (American Chemical The "editorial style" of the Journal essentially Society, 1974). Citations of abstracts, theses, "un- follows the CBE Style Manual (3rd ed., AIBS, 1972). published data," "personal communication," and, For bacterial genetic systems, the recommendations "in press" will not be accepted in the Literature of Demerec et al. (Genetics 54:61, 1966) should be Cited. followed, with the limited modifications of Taylor Tables. Each table should be typed on a separate and Trotter, Sanderson, and Low (Bacteriol. Rev. page. The data should be arranged so that columns 36:504-524; 558-586; and 587-607, 1972). For yeast, of like material read down, not across. Explanatory Chlamydomonas, and several fungal species, sym- footnotes are permitted, but detailed descriptions bols such as those given in Handbook of Micro- of the experiments are not. The materials and biology (1974, A. I. Laskin and H. A. Lechevalier, methods used to gain the data should properly eds., CRC Press, Cleveland, p. 665-711, Vol. IV) remain in the section of that name. should be employed. For genetic nomenclature, use Figures. A complete set of figures, preferably italics consistently for genotype symbols. Greater glossy photographs, should accompany each of the leniency can be allowed for phenotypic symbols. two copies of the manuscript. Graphs (submit as However, they should not be italicized. The pre- photographs) should be finished drawings not need- ferred convention is to use the same three-letter ing further artwork or type-setting. Absolutely no symbols in Roman type with the first letter capital- part of a graph should be typewritten (except the ized. legend, which should be typed on a separate page). Biochemical nomenclature, including abbrevia- Most graphs will be reduced to one-column width, tions and symbols, should follow the recommenda- and all elements in the drawing should be prepared tions of the IUPAC-IUB Commission on Biochemi- to withstand this reduction. The legend ofthe figure cal Nomenclature (CBN) and the Instructions to should provide enough information so that the Authors of J. Biol. Chem. and Arch. Biochem. figure is understandable without reference to the Biophys. (first issue of each year), which are based text. Experimental details from Materials and upon the CBN Recommendations and contain a list Methods should not be repeated in figure legends. thereof. Reprints of these Recommendations and Figure ordinate and abscissa scales, and table advice on biochemical and chemical nomenclature column headings, should avoid ambiguous use of are available from the NRC Office of Biochemical numbers with exponents (see p. 140, CBE Style Nomenclature (W. E. Cohn, Director), Biology Manual). Division, Oak Ridge Nat'l. Lab., Box Y, Oak Ridge, Nomenclature of microorganisms. In general, Tenn. 37830 (phone: 615-483-8611, Ext. 3-7514). the nomenclature presented in Bergey's Manual of Normally, abbreviations (except those of standard Determinative Bacteriology (8th ed., 1974) is used. units of measurement and symbols of the elements) If an author challenges this nomenclature, his own should be defined and introduced parenthetically at judgment will be followed, but the name in Bergey's first use in the text. Enzyme activities should be Manual should follow in parentheses the first time expressed in the terms set out in CBN's Enzyme the name is used in the text and in the Abstract. Nomenclature (1972) (Elsevier Scientific Publishing Papers which include extensive taxonomic material Co.). Lengths, weights, volumes, and molarities (e.g., description of new taxa) will not be published should make use of the prefixes m, ,u, n, and p (for in this journal. The proper place for publication of 10-3, 10-6, 10-9, and 10-12, respectively), where taxonomic material is the International Journal of applicable, avoiding such compound prefixes as m,u Systematic Bacteriology (IJSB), which is published and j±A. Metric units should be employed as much by the ASM for the International Association of as possible. The Editors reserve the privilege of Microbiological Societies. If the main thrust of such editing manuscripts to make them conform to the a paper is not taxonomy, the paper should be di- above-mentioned stylistic conventions. As a rule, vided, the taxonomy portion being submitted to all bacterial growth curves should be presented as IJSB and the other portion to this journal. semilogarithmic plots (log x vs. time). Notes. The accepted form for Notes is somewhat Form of manuscript. All parts of the manuscript different from the foregoing. Contributors should should be typed double-space or, preferably, triple- consult a recent issue of the Journal for style. Notes space. Most manuscripts can and should be divided should not exceed 500 words. The Abstract should into the following sections: Abstract, Introduction, not exceed 25 words. Materials and Methods, Results, Discussion, Ac- Copyright. Once a paper has been published in knowledgments, and Literature Cited. the Journal, which is a copyrighted publication, the Title. A single statement of the subject of a paper legal ownership of all parts of the paper, including is preferred to a main title-subtitle arrangement. A the illustrations, has passed from the author to the short version ofthe title (no more than 46 characters ASM. If the same author, or any author, wishes to and spaces) should be supplied for use as a running republish material previously published in the head. Journal, he must first receive written permission from ASM. 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Advertising in thisjournal is limited to products anid services believed to be if interest to the readers. Honever. ASM does not test n-or examinle advertised products nor claims related thereto. Therefore. ASM endorsenment or approval of advertised products should not be inferred. AUTHOR INDEX VOLUME 126

Adams, James N., 1104 Crosa, Jorge H., 454 Hacking, Andrew J., 1166 Adhya, Sankar, 1108 Culbertson, Michael R., 232, 243 Halegoua, Simon, 183 Akagi, J. M., 733 Currier, T. C., 157 Hall, Barry G., 536 Alderete, John F., 327 Haller, M. N., 417 Allen, G. V., 1020 Daneo-Moore, L., 192 Hamilton, W. Allan, 1224, 1232 Amelunxen, R. E., 520 Das, H. K., 1042 Hanawalt, Philip, 814 Anagnostopoulos, C., 609 Davies, John K., 1347 Hancock, Robert E. W., 1347 Anderson, James J., 80, 257, 264 Davis, Minh-Tam B., 1136 Harriman, Philip D., 1141 Andreoli, Peter M., 861 Davis, Patrick J., 544 Hartig-Beecken, I., 951 Armstrong, Karen A., 38, 48 Deal, S. J., 751 Hattman, Stanley, 990 Asato, Yukio, 550 de Graaff, Johannes, 439 Havekes, Louis M., 593 Aten, Jacob A., 384 Deltour, R., 937 Hebeler, Bruce H., 1180, 1186 Atryzek, Vladimir, 1005 DeMoss, John A.,.478 Heffernan, Laurel, 1119, 1132 Dempsey, Walter B., 166 Heincz, Maria C., 132 Bailey, R. B., 1012 DeVoe, I. W., 400 Helinski, Donald R., 447 Bass, Richard, 1119 Dobson, P. R., 1020 Hellermann, G. R., 785 Beck, Barbara, 1250 Doctor, Freny, 997 Hempfling, Walter P., 251 Bennett, P. M., 1 Donahue, Thomas F., 232, 243 Henry, Susan A., 232, 243 Benson, Spencer, 794 Doolittle, W. F., 1020 Herman, Robert K., 38 Berger, Lisbeth, 990 Doyle, R. J., 140 Hermann, T. E., 743 Bergquist, P. L., 1063 Drake, H. L., 733 Hershfield, Vickers, 447 Bhatti, A. R., 400 Dubnau, D., 429 Hirashima, Akikazu, 183 Billen D 785 Dworsky, Paul, 64 Hirsch, C. F., 13, 24 Bird, Robert E., 1215 Hochhauser, Steven J., 1082 Bloom, Gunnar D., 969 Edson, Clark M., 799 Hochstadt, Joy, 312 Body, Barbara A., 1149 Egi, Yoshiko, 1030 Hoekstra, Wiel P. M., 593 Bonen, L., 1020 Eisenberg, Roselyn J., 1344 Holden, Joseph A., 1141 Boos, W., 951 Elbein, Alan D., 553 Hou, Ching-Tsang, 516, 1017 Borbely, G., 630 Elchisak, Mary, 1344 Howard-Flanders, P., 646 Bossinger, June, 198 Eleuterio, Marianne, 1014 Boyer, Herbert W., 447 Elmros, Theodor, 969 Brody, Stuart, 799 Elwell, Lynn P., 439 Imae, Yasuo, 907, 914 Broquist, Harry P., 338, 1207 Englesberg, Ellis, 1119 Ingebretsen, Ole C., 1075 Ensign, J. C., 13, 24 Ingram, J. M., 400 Brownell, George H., 1104 Inouye, Masayori, 183 Brownstein, Bernard H., 1149 Falaschi, Arturo, 108 Ishii, Naoaki 529 Budd, K., 690 Falkinham, Joseph 0., III, 1194 Ives, David i., 1136 Burke, Kathleen A., 225 Falkow, Stanley, 439, 454 Izumori, Ken, 553 Burman, Lars G., 969 Fan, David P., 48 Butler, R. Christopher, 511 Farkas, G. L., 630 Feldner, Jurgen, 100 Jackman, Lance E., 312 Calhoun, David H., 56 Felix, A., 1017 James, Eric, 487 Calmes, Robert, 751 Fenske, Jane D., 501 James, Philip M., 487 Canosi, Umberto, 108 Ferrari, F. A., 533 Jarvis, Audrey W., 1232 Caro, Lucien, 1215 Firshein, William, 777 Jenkins, Arthur, 587 Chaffin, W. LaJean, 771 Fisher, W. D., 977, 985 Juretic, Davor, 542 Chakrabarty, A., 410 Flawia, Mirtha M., 91 Chandler, Michael, 1215 Foster, Gayle C., 1297 Kadota, Hajime, 1342 Chang, H. C. P., 1002 Foster, R. Scott, 1297 Kahn, Michael, 814 Chapman, Ann, 1351 Kalghatgi, Krishna K., 568 Chen, Chen-Lo H., 511 Frick, Kevin, 7 Kapoor, V., 1245 Chen, Kuo-Chun, 883 Friedrich, Barbel, 712, 723 Kawamoto, Susumu, 919 Chow, L., 447 Friedrich, Cornelius G., 712, 723 Kawasaki, Takashi, 1030 Cintr6n, Nitza M., 1082 Fukui, Saburo, 919 Keisel, Nicole, 294 Clark-Walker, G. D., 959 Garro, Anthony J., 556 Kenny, George E., 501 Cleveland, R. F., 192 Glaser, Donald A., 1089 Khachatourians, R. L., 977 Cohen, Jeffrey, 177 Glauert, Audrey M., 869 Kido, Toshiko, 1261 Cole, Roger M., 1285 Goldberg, I. D., 520 Kidson, Chev, 587 Collins, Mary Lynne Perille, Gottesman, Max, 1108 Koch, Arthur L., 140 1316, 1326 Greene, Milford, 777 Konings, Ruud N. H., 861 Collins, Stephen H., 1224, 1232 Grimminger, Herbert, 100 Kooistra, Jan, 31 Collmer, Alan, 806 Guengerich, F. Peter, 338 Krzyzek, Richard A., 348, 365 Cooper, Terrance G., 198 Gustafson, Mark E., 544 Kubitschek, H. E., 140 Curiale, Michael S., 661 Kulpa, Charles F., Jr., 467 Curtiss, Roy, III, 1194 Haag, Steven M., 1285 Kung, Fang-Chien, 1089 Crawford, Irving P., 547 Hackett, Charles J., 883 Kwon-Chung, K. J., 524 i ii AUTHOR INDEX J. BACTERIOL. Lamborg, Marvin, 806 Ornston, L. Nicholas, 272 Silbert, David F., 1351 Lampky, J. R., 1278 Orr, E., 895, 903 Silhavy, T. J., 951 Landau, J. V., 654 Osumi, Masako, 919 Siv6k, B., 630 Larson, Robert J., 282 Oxender, Dale L., 80 Slater, Martin L., 1339 Lascelles, June, 225 Sleytr, Uwe B., 377, 869 Lavin, Martin F., 587 Palchaudhuri, Sunil, 410 Small, Gary D., 31 Leive, Loretta, 467 Panos, Charles, 300 Smith, Walter P., 654 Lemke, P. A., 417 Park, James T., 1250 Soda, Kenji, 1261 Leong, John, 823 Park, Myung Hee, 1096 Sogin, Stephen J., 771 Lillard, Marjorie O., 516 Parke, Donna, 272 Sorger, G. J., 1002 Lin, E. C. C., 1166 Parkinson, John S., 758 Sprouse, Carole, 556 Lin, Jim Jung-ching, 147 Parks, L. W., 1012 Strominger, Jack L., 907, 914 Lindahl, T., 646 Pate, Jack L., 282 Struhl, Kevin, 739 Lindsay, Raymond J., 1232 Patel, Ramesh, 1017 Subba Rao, P. V., 568 Linnett, Paul E., 213 Petryna, Marta M., 661 Subrahmanyam, C. S., 1042 Ljungquist, S., 646 Pohlman, Timothy, 1351 Swenson, P. A., 977, 985 Lopez, M. E., 533 Polsinelli, M., 533 Sypherd, Paul S., 1266 Loppes, R., 937 Pope, D. H., 654 Lusk, Joan E., 1096 Popkin, Terry J., 524 Tabita, F. Robert 1271 Luttropp, Linda K., 454 Potter, R., 1245 Tanaka, Atsuo, 919 Proctor, Alan R., 547 Tellez-In6n, Maria T., 91 McArthur, C. R., 959 Pugsley, Anthony P., 1052 Terenzi, H6ctor F., 91 McFadden, Bruce A., 1271 Thomassen, Elisabeth, 999 McFall, Elizabeth, 132 Quay, Steven C., 80 Thorne, Kareen J. I., 377 MacGregor, C. H., 122 Tipper, Donald J., 213 McKeever, W. Galen, 634 Rake, Adrian V., 1305 Tomasz, Alexander, 1113 McKeown, Michael, 814 Ranhand, Jon M., 205 Torres, Hector N., 91 Magasanik, Boris, 739 Raymond, Jonathan, 1089 Trowsdale, J., 609 Mainzer, Stanley E., 251 Rebouche, Charles J., 1207 Truman, Penelope, 1063 Maloney, Peter C., 327 Reeves, Peter, 1052, 1347 Mannel, D., 579 Reusch, Victor M., Jr., 300 Uchida, Aritsune, 1342 Manson, Michael D., 668, 679 Reyes, Oscar, 1108 Udvardy, J., 630 Mantsala, Pekka, 539 Richmond, M. H., 1 Marino, R., 417 Riley, Monica, 559 van Iterson, Woutera, 384 Marrs, Barry, 619 Roehl, R. A., 852 Veltkamp, Eduard, 861 Matagne, R. F., 937 Rogers, Palmer, 348, 365 Vinopal, R. T., 852 May, Maureen S., 990 Rogers, Stephen R., 257, 264 Vold, Barbara, 294 Mayer, H., 579 Rood, Julian I., 831, 845 Mazza, Giorgio, 108, 533 Rosazza, John P., 544 Wall, Judy D., 1141 Mendelson, Neil H., 1285 Rosenberger, R. F., 895, 903 Walmsley, Roger H., 222 Miller, A. G., 690 Rowe, J. J., 520 Wayne, Rush, 7 Miller, Lynn, 1012 Rudd, Joanne, 1344 Weisburd, Martin, 177 Mills, Dallice, 661 Weiss, Bernard, 1082 Mindich, Leonard, 177 Sabet, Sohair F., 601 Weiss, Richard L., 1173 Mitchell, Mike, 553 Sadaie, Yoshito, 1037 Wetmur, James G., 556 Modi, V. V., 997 Saksena, K. N., 417 Whang, H. Y., 579 Molholt, Bruce, 563 Sanemori, Hiroshi, 1030 White, Brian J., 1082 Montoya, Alice L., 699 Sanner, Tore, 1075 Wicken, A. J., 192 Mooney, Duane T., 1266 Schaeffer, Pierre, 1342 Wilcox, Gary, 1132 Moore, Steven K., 487 Schenley, R. L., 977, 985 Wilkinson, Russell G., 831, 845 Morimyo, Mitsuoki, 529 Scher, B., 429 Willetts, Neil S., 166 SChlagman, Samuel, 990 Wilson, David B., 1156 Narui, Kikuko, 1037 &Chlegel, Hans G., 712, 723 Wilson, Dorothy M., 327 Natter, William, 487 Schmidt, G., 579 Wilson, P. W., 743 Neidhardt, Frederick C., 634 Scott, Randolph H., 478 Wilson, T. Hastings, 327 Neilands, J. B., 7, 823 Sens, Don, 487 Wong, Brenda B., 1096 Nester, E. W., 157, 699 Setlow, Jane K., 31 Wood, Nancy B., 1305 Neter, E., 579 Seto, Hiroyuki, 1113 Wu, Henry C., 147 Neuhard, Jan, 999 Shapanka, Roslyn, 31 Newman, E. B., 1245 Shapiro, James, 794 Yamamoto, Tatsuo, 1261 Ng, Ah-Kau, 511 Shapiro, Lucille, 1305 Yanofsky, Charles, 668, 679 Niederman, Robert A., 131L6, Sheppard, David E., 1014 Yasuhara, Shigeki, 919 1326 Shida, Hisatoshi, 1025 Yen, Huei-Che, 619 Nijkamp, H. John J., 861 Shimazu, Yoshie, 529 Young, Frank E., 1180, 1186 Nowotny, Alois, 511 Shinozawa, Takao, 1025 Young, M., 928 Shockman, G. D., 192 O'Conner, R. M., 959 Shugart, Lee, 1009 Zalkin, Howard, 539 Ordal, George W., 72, 706 Siccardi, Antonio G., 108, 533 Zipkas, David, 559 SUBJECT INDEX VOLUME 126 Acetyl coenzyme A carboxylase Arginine temperature-sensitive mutation in E. coli, 1351 compartmentation and metabolism in Neuro- Adenosine 5'-triphosphate spora, 1173 lactate dehydrogenase activity, regulation of, expression of the argECBH operon, regulation of, 1344 348 Adenosine 5'-triphosphate-dependent nuclease stability and size ofargECBH mRNA, 365 H. influenzae mutants deficient in, 31 Arginine deiminase Adenosine 5'-triphosphate synthesis M. hominis growth, 501 E. coli, 327 Arginine transducing deoxyribonucleic acid protonmotive force as the source of energy, 327 endonucleasesEcoRI and SmaR, cleavage by, 487 Agaricus bisporus development Aromatic amino acid and histidine biosynthesis, en- study by fluorescent microscopy and SEM, 417 zyme common to Agrobacterium B. subtilis, 699 large plasmids, 157 AS- cyanophage Alanine transport UV inactivation and photoreactivation in A. nid- E. coli, 1232 ulans, 550 proton movements, 1232 Ascospores in Sordaria brevicollis, 883 Alcaligenes eutrophus Aspergillus nidulans chorismate mutase-prephenate dehydratase, 712, nuclear replication mutants, 895, 903 723 Asticcacaulis biprosthecum prephenate dehydrogenase, 712, 723 glucose transport in prosthecae, 282 Alcohol dehydrogenase, plasmid-determined Autolysis P. putida, 794 N. gonorrhoeae, 969, 1186 Alkaline phosphatase participation in P. aeruginosa cell division, 400 Bacillus megaterium Alkaline phosphatase activity ribosomal precursor particles, 1149 dissociation ofdeveloping slime mold cells, 1005 Bacillus polymyxa nitrogenase, 743 Allophanate hydrolase, induction of Bacillus sphaericus Saccharomyces, 198 peptidoglycan synthetase activity in sporangia Amber uvrA and uvrB mutants ofEscherichia coli, and forespores, 213 529 Bacillus sphaericus spores Amino acid starvation cortex content, 907 E. coli translation, 1042 Bacillus stearothermophilus Amino acid transport mutants blocked in catabolic function, 520 regulatory loci in E. coli, 80 Bacillus subtilis Aminoacyl-transfer ribonucleic acid synthetases cell development from spores, 384 growth rate modulation, 634 cortex content of mutants, 914 Ammonia-sensitive mutant ofKlebsiella aerogenes, DNA replication inhibitors and recombination, 739 108 Ammonium ions endonuclease, Mn2+-stimulated, 429 N. crassa nitrite reductase, 1002 gene expression during sporulation, 928 amp genetic structure of strains carrying trpE26 muta- translocation in E. coli, 1 tion and strain 168, 609 Anacystis nidulans histidine and aromatic amino acid biosynthesis, AS-1 cyanophage, UV inactivation and photoreac- 699 tivation of, 550 Mn2+-stimulated endonuclease, 429 virus-induced DNase activity, 630 mutator activity in dna-8132 strains, 1037 Aphanocapsa purine markers, 533 novel, stable RNA species, 1020 recombination-deficient phenotype of recC strains Apurinic sites in deoxyribonucleic acid associated with an SP02 prophage, 556 MMS-sensitive mutant ofE. coli, 646 repair deficiency in dna-8132 strains, 1037 ara regulon in Escherichia coli repellents of, 72 catabolite deactivation, 1119, 1132 spore coat protein, 1342 argECBH messenger ribonucleic acid sporulation, gene expression during, 929 arginine effect on stability and size, 365 sporulation mutants, 928 argECBH operon ofEscherichia coli thermal prophage inducibility in dna-8132 regulation by arginine, 348 strains, 1037 iii iv SUBJECT INDEX J. BACTERIOL. tumbling, control by divalent cation, 706 Clo DF13 plasmid DNA-directed synthesis, 861 undermethylated tRNA, 294 Clostridium perfringens Zebra structures, 1285 hemagglutinin sialidase, 831, 845 Bacteriochlorophyll biosynthesis, genes for Clostridium thermohydrosulfuricum R. capsulata, 619 cell walls, 869 Bacteriophage cross-resistance with colicins surface layers, characterization of, 377 E. coli, 1347 Clostridium thermosaccharolyticum Bacteriophage lambda adsorption surface layers, characterization of, 377 E. coli mutant, 1025 Colicin Barotolerant protein synthesis siderophore protection against, 7 ion concentration, 654 Colicin cross-resistance with phages Beta-lactamase-specifying plasmids from Haemo- E. coli, 1347 philus influenzae, 439 Colicin K Biosynthesis of a membrane lipoprotein E. coli membrane functions, effect on, 601 E. coli, 183 Common antigen, enterobacterial Bisulfite reductase activity of Desulfovibrio vul- role of lipopolysaccharide gene for immunogenic- garis, 733 ity, 579 Blastocladiella emersonii Compartmentation of arginine in Neurospora, 1173 enzyme level variation related to energy metabo- Competence development lism, 1075 effect of pH, 205 Blue-green bacteria S. sanguis, 205 novel, stable RNA species, 1020 Conjugation E. coli, 559 Calcium method for interruption of, 559 DNA uptake in pneumococci, 1113 Conjugation-deficient mutants of Escherichia coli, Candida albicans 1194 germ tube formation, 771 Cortex content Carnitine biosynthesis in Neurospora crassa, 1207 B. sphaericus spores, 907 Carotenoid biosynthesis, genes for B. subtilis mutants, 914 R. capsulata, 619 Cross-resistance between phages and colicins Catabolite deactivation E. coli, 1347 ara regulon ofE. coli, 1119, 1132 Crown gall tumorigenesis Catabolite repression, hypersensitivity to plasmids in Agrobacterium, 157 E. coli L-arabinose operon, 1014 Cryptococcus neoformans septal complex, 524 Caulobacter C27 sterol-accumulating yeast strains DNA structure, 1305 enzymatic analysis, 1012 Cell age versus metal ion content Cyanide-resistant respiration E. coli, 1089 N. crassa mutant, 542 Cell development from Bacillus subtilis spores, 384 Cyanophage AS-1 Cell walls UV inactivation and photoreactivation in A. nid- C. thermohydrosulfuricum, 869 ulans, 550 C. thermosaccharolyticum, 869 Cyclic adenosine 3',5'-monophosphate Centrifugal separation of cell populations, 977 N. crassa morphogenesis, control of, 91 cheA, cheE, and cheC chemotaxis ofE. coli, 758 dcd, deletion of Chemotactic repellents ofBacillus subtilis altered deoxyribonucleotide pools in P2 eductants recognition sites, 72 ofE. coli, 999 Chemotaxis Deoxycytidine control of tumbling by divalent cation, 706 incorporation into DNA deoxycytidylate in L. aci- E. coli mutants, 758 dophilus, 1136 Chlamydomonas reinhardi Deoxyribonuclease activity, virus induced phosphatases, 937 A. nidulans, 630 Chorismate mutase-prephenate dehydratase from Deoxyribonucleic acid Alcaligenes eutrophus, 712, 723 calcium-requiring step in uptake in pneumococci, Chromatophore fractions 1113 R. rubrum, 1326 Caulobacter, 1305 Chromobacterium violaceum E. coli mutant deficient in an endonuclease spe- indole-3-carboxylic acid formation, 544 cific for apurinic sites, 646 Chromosomes, folded P. putida plasmids, 410 E. coli, 64 replication inhibitors and recombination, 108 Circular deoxyribonucleic acid Deoxyribonucleic acid, circular T. glabrata, 959 T. glabrata, 959 Cl- influx and efflux byNeocosmospora vasinfecta Deoxyribonucleic acid-cytosine methylase exogenous sugars and polyols, 690 methylation protects against cleavage by Cloacin DF13-specific proteins R-EcoRII, 990 VOL. 126, 1976 SUBJECT INDEX v Deoxyribonucleic acid deoxycytidylate argECBH mRNA, effect of arginine on, 365 incorporation of dCyd, 1136 argECBH operon, regulation of expression, 348 Deoxyribonucleic acid ligase ATP synthesis, 327 membrane fraction from pneumococci, 777 catabolite repression in the L-arabinose operon, repair synthesis in E. coli, 785 hypersensitivity to, 1014 Deoxyribonucleic acid polymerase cell age versus metal ion content, 1089 repair synthesis in E. coli, 785 centrifugal separation of cell populations, 977 Deoxyribonucleic acid uptake cheA, cheB, and cheC genes and their role in effect of pH, 205 chemotaxis, 758 S. sanguis, 205 chromosome replication origin, change in, 1215 Deoxyribonucleotide pools, altered Clo DF13-specific proteins, 861 P2 eductants ofE. coli, 999 colicin K and membrane functions, 601 Desulfovibrio vulgaris colicins, protection against, 7 bisulfite reductase activity, 733 conjugation-deficient mutants, 1194 Dictyostelium discoideum conjugation, method for interruption of, 559 alkaline phosphatase activity, 1005 cross-resistance between phages and colicins, 1347 Dimethyl ether oxidation, inhibition of dnaA mutation, suppression by R.100.1, 1215 M. capsulatus, 1017 essential genes in the metB-malB region, 48 M. trichosporium, 1017 excision, 985 Dimorphism in Mucor racemosus exonuclease III, structural gene for, 1082 volatile factor, 1266 F-Con- mutant, 593 Divalent cation folded chromosomes, 64 control of tumbling by divalent cation, 706 formate-nitrate electron transport pathway, 478 dnaA mutation fucose pathway, disruption of, 1166 suppression by R.100.1, 1215 glucose phosphotransferase function in mutants, 852 ebgA° gene glutamate synthase, active subunits of, 539 methyl galactosidase activity, 536 glycerol phosphate transport protein, 951 EcoRI and SmaR restriction glycine synthesis, 1245 k80dargF DNA, 487 guanine-xanthine phosphoribosyltransferase, Ectothiorhodospira halophila mutants deficient in, 1141 ribulose 1,5-bisphosphate carboxylase, 1271 hypersensitivity to catabolite repression in the L- Endonuclease-digested plasmids arabinose operon, 1014 N. gonorrhoeae, 1297 iron uptake in colicin ,8-resistant mutants, 1052 Endonuclease, Mn2+-stimulated isolation of mutants deficient in essential genes, B. subtilis, 429 38 Endonucleases EcoRI and SmaR lambda phage adsorption, 1025 arginine transducing DNA, cleavage of, 487 lipopolysaccharide insertion into outer mem- Endonuclease specific for apurinic sites in deoxyri- brane, 467 bonucleic acid lipoprotein mutants, 147 MMS-sensitive mutant ofE. coli, 646 magnesium transport in mutants, 1096 Endotoxic glycolipids mating-pair formation, temperature dependence differences in the lipid moiety, 511 of, 222 Endotoxins membrane functions and colicin K, 601 relationship of structure to function, 511 membrane lipoprotein biosynthesis, 183 Enteric bacteria metal ion content verus cell age, 1089 siderophore iron transport, 823 methylation protects against cleavage by tryptophan operon regulation, 679 R-EcoRII, 990 Enterobacterial common antigen, immunogenicity (S-methyl galactoside transport, entry and exit re- of actions of, 1156 lipopolysaccharide gene, 579 methyl methane sulfonate-sensitive mutant, 646 Entry and exit reactions missense mutations in lacZ, 1063 ,8-methyl galactoside transport in E. coli, 1156 murein hydrolases during cell division cycle, 1250 Enzyme levels in Blastocladiella emersonii, 1075 murein lipoprotein mutants, 147 Escherichia coli nucleoside transport, 814 acetyl CoA carboxylase, temperature-sensitive phosphotransferase function in mutants, 852 mutation affecting, 1351 protonmotive force, 1224 altered deoxyribonucleotide pools in P2 eductants, pyrimidine dimer excision, 985 999 ribosomal protein cistrons, extragenic suppres- amber uvrA and uvrB mutants, 529 sion of, 563 amino acid starvation and translation, 1042 R-plasmid replication, 454 amino acid transport, loci affecting, 80 separation of cell populations, 977 aminoacyl-tRNA synthetases, regulation of, 634 D-serine deaminase cleavage and aggregation, 132 ara regulon and catabolite deactivation, 1119, siderophore iron transport, 823 1132 siderophore protection against colicins, 7 vi SUBJECT INDEX J. BACTIERIOL. soluble precursor of nitrate reductase, 122 threonine deaminase from an E. coli nonsense temperature effect on yield and maintenance, 251 mutant, 100 threonine catabolism, 1245 Hansenula mrakii threonine deaminase, 56 nitroalkane-oxidizing enzyme, 1261 threonine deaminase from a nonsense mutant, 100 Heat activation of spores thymidine uptake and utilization, 814 S. viridochromogenes, 24 translation and amino acid starvation, 1042 Hemagglutinin sialidase from Clostridium perfrin- translocation of DNA carrying amp, 1 gens, 831, 845 tryptophan operon, 668, 679 Histidine and aromatic amino acid biosynthesis, en- X-ray-induced repair synthesis, 785 zyme common to xthA, 1082 B. subtilis, 699 Excision Hypersensitivity to catabolite repression in the L- surviving and nonsurviving E. coli, 985 arabinose operon Exonuclease III E. coli, 1014 xthA, mapping of, 1082 Hypoxanthine uptake Extragenic suppression ofribosomal protein cistrons S. typhimurium membrane vesicles, 312 E. coli, 563 Immunogenicity of enterobacterial common antigen Fatty acid transport lipopolysaccharide gene, 579 N. asteroides, 751 Indole-3-carboxylic acid Filobasidiella neoformans septal complex, 524 C. violaceum, formation by, 544 Folded chromosomes from Escherichia coli, 64 Inositol biosynthesis, control of Forespores ofBacillus sphaericus S. cerevisiae, 232, 243 peptidoglycan synthetase activity, 213 Inositol-phosphate synthetase Formate-nitrate electron transport pathway S. cerevisiae mutants, 243 E. coli, inactive components in, 478 Iron deposition inSphaerotilus discophorus, 257, 264 Fructose 1,6-diphosphate Iron uptake lactate dehydrogenase activity, regulation of, E. coli colicin B-resistant mutants, 1052 1344 Isoleucine-valine loci in Rhizobium japonicum, 997 Fucose pathway, disruption of genetic adaptation to propanediol as a carbon source in E. coli, 1166 f3-Ketoadipate uptake system in Pseudomonas pu- Fungi tida mutants lysine catabolism, 338 constitutive synthesis of enzymes, 272 Klebsiella aerogenes Galactosamine metabolism in Neurospora ammonia-sensitive mutant, 739 crassa,799 tryptophan operon, 679 gal operon Klebsiella pneumoniae suppression of polarity, 1108 nitrogen fixation genes, 806 Gene expression Kloeckera, methanol grown B. subtilis sporulation, 928 microbodies, 919 Genetic structure B. subtilis strains, 609 Lactate dehydrogenase activity Germ tube formation of Candida albicans, 771 R. dentocariosa, regulation in, 1344 Glucose phosphotransferase Lactobacillus acidophilus impaired function in E. coli mutants, 852 dCyd incorporation into DNA deoxycytidylate in Glucose transport L. acidophilus, 1136 A. biprosthecum prosthecae, 282 lacZ Glutamate synthase ofEscherichia coli missense mutations in E. coli, 1063 active subunits, 539 Lambda phage adsorption Glycerol phosphate transport protein E. coli mutant, 1025 E. coli, 951 Leucine loci in Rhizobiumjaponicum, 997 Glycine synthesis Lipid E. coli, 1245 wall autolysis in S. faecalis, inhibition of, 192 L-threonine dehydrogenase Lipopolysaccharide Group H streptococci, competence of, 205 insertion into E. coli outer membrane, 467 Guanine uptake Lipopolysaccharide gene S. typhimurium membrane vesicles, 312 immunogenicity of the enterobacterial common Guanine-xanthine phosphoribosyltransferase antigen, 579 E. coli mutants, 1141 Lipoprotein mutants ofEscherichia coli, 147 Lipoteichoic acid Haemophilus influenzae wall autolysis in S. faecalis, inhibition of, 192 beta-lactamase-specifying plasmids, 439 Lysine catabolism in fungi, 338 mutants deficient in ATP-dependent nuclease, 31 Lysine conversion to e-N-trimethyllysine Half-of-the-sites reactivity N. crassa, 1207 VOL. 126, 1976 SUBJECT INDEX vii Magnesium transport in Escherichia coli mutants, Near-ultraviolet light damage to permease 1096 E. coli, 140 Manganese-stimulated endonuclease from Bacillus Neisseria gonorrhoeae subtilis, 429 autolysis, 969, 1186 Mapping of leucine and isoleucine-valine loci in R. endonuclease-digested plasmids, 1297 japonicum, 997 peptidoglycan, 1180 Mating-pair formation in Escherichia coli Neocosmospora vasinfecta temperature dependence, 222 C1- influx and efflux, 690 Membrane Neurospora R. rubrum, 1082, 1089 arginine, compartmentation and metabolism of, Membrane-associated folded chromosomes 1173 E. coli, 64 Neurospora crassa Membrane functions, effect of colicin K on carnitine biosynthesis, 1207 E. coli, 601 cyanide-resistant respiration in mutants, 542 Membrane lipoprotein galactosamine metabolism, 799 Puromycin-resistant biosynthesis inE. coli, 183 morphology, control of, 91 Membrane transport in Escherichia coli nitrite reductase, effect of ammonium ions on, near-UV inactivation of permease system, 140 1002 Metal ion content versus cell age nif E. coli, 1089 K. pneumoniae, 806 metB-malB Nitrate reductase activity E. coli essential genes, 48 S. aureus heme mutants, 225 Methane oxidation, inhibition of Nitrate reductase in Escherichia coli M. capsulatus, 1017 soluble precursor, evidence for, 122 M. trichosporium, 1017 Nitrite reductase induction in Neurospora crassa Methylation protects against cleavage by R EcoRII, ammonium ions, effect of, 1002 990 Nitroalkane-oxidizing enzyme from Hansenula Methylation, sective mrakii, 1261 incorrect hypothesis, 1009 Nitrogenase Methyl galactosidase activity: alternative evolu- B. polymyxa, 743 tionary destination for the ebgA° gene, 536 Nitrogen fixation genes in Klebsiella pneumoniae, 13-Methyl galactoside transport inEscherichia coli 806 entry and exit reactions, 1156 N mutations in bacteriophage lambda Methyl methane sulfonate-sensitive mutant of suppression of polarity, 1108 Escherichia coli, 646 Nocardia asteroides Methylococcus capsulatus fatty acid transport, 751 dimethyl ether and methane oxidation, inhibition Nocardial lysogens, matings of of, 1017 nocardiophage OEC, inheritance of, 1104 Methylosinus trichosporium Nocardiophage OEC dimethyl ether and methane oxidation, inhibition inheritance in matings ofnocardial lysogens, 1104 of, 1017 Nonsense suppressor mutants ofPseudomonas, 177 Microbodies of methanol-grown Kloeckera, 919 Novel, stable ribonucleic acid species accumulated Micrococcus denitrificans by Aphanocapsa thiamine pyrophosphate synthesis, 1030 nonribosomal nature, 1020 Micrococcus radiophilus Nuclear replication UV-induced damage, repair of, 587 A. nidulans mutants, 895, 903 Mini-ColEl plasmid, 447 Nuclear staining method Missense mutation in lacZ ofEscherichia coli, 1063 S. cerevisiae, 1339 Mithramycin Nucleoside transport in Escherichia coli, 814 nuclear staining method for S. cerevisiae, 1339 Morphogenesis ofNeurospora crassa Outer membrane ofEscherichia coli control by cyclic AMP, 91 lipopolysaccharide insertion, 467 Mucor racemosus volatile factor involved in dimorphism, 1266 Peptidoglycan Murein hydrolases N. gonorrhoeae, 1180 E. coli cell division cycle, 1250 Peptidoglycan formation Murein lipoprotein S. pyogenes L-form, 300 E. coli mutants, 147 Peptidoglycan synthetase activity Mutator activity B. sphaericus sporangia and forespores, 213 B. subtilis dna-8132, 1037 Periplasmic protein Mycobacterium smegmatis glycerol phosphate transport system ofE. coli, 951 isomerization of D-ribose and L-rhamnose is cata- Permease lyzed by the same enzyme, 553 near-UV inactivation in E. coli, 140 Mycoplasma hominis growth pH arginine deiminase, 501 effect on competence development and DNA up- Viii SUBJECT INDEX J. BACTERIOL. take in S. sanguis, 205 Pseudomonas L-Phenylalanine ammonia-lyase regulation nonsense suppressor mutants, 177 R. solani, 568 Pseudomonas aeruginosa Phenylalanine-tyrosine alkaline phosphatase participation in cell divi- enzymes in A. eutrophus, 712 sion, 400 regulation in A. eutrophus, 723 Pseudomonas bathycetes 480dargF deoxyribonucleic acid ions and barotolerant protein synthesis, 654 EcoRI and SmaR restriction, 487 Pseudomonas fluorescens 4EC nocardiophage ions and barotolerant protein synthesis, 654 inheritance in matings ofnocardial lysogens, 1104 Pseudomonas putida Phosphatases of Chlamydomonas reinhardi, 937 constitutive synthesis ofenzymes of the protocate- Phosphofructokinase mutants ofEscherichia coli chuate pathway and the /8-ketoadipate uptake impaired phosphotransferase function, 852 system, 272 Phosphotransferase plasmid alcohol dehydrogenase, 794 impaired function in E. coli mutants, 852 P. putida Photoreactivation plasmid DNA, 410 AS-1 cyanophage in A. nidulans, 550 protocatechuate 3,4-dioxygenase isofunctional en- Plasmid alcohol dehydrogenase in Pseudomonas pu- zymes, 516 tida, 794 tryptophan synthase, autogenous regulation of, Plasmid, beta-lactamase-specifying 547 H. influenzae, 439 Purine markers in Bacillus subtilis, 533 Plasmid co-integrates of X and R100, 166 Purine utilization in bacteria, 312 Plasmid deoxyribonucleic acid Puromycin resistance of membrane lipoprotein bio- P. putida, isolation from, 410 synthesis Plasmids, endonuclease-digested E. coli, 183 N. gonorrhoeae, 1297 Pyrimidine dimer excision Plasmids, large surviving and nonsurviving E. coli, 985 Agrobacterium, 157 Pneumococcus R.100.1 DNA ligase activity in membrane fraction, 777 suppression of dnaA mutation, 1215 DNA uptake, calcium-requiring step in, 1113 R-EcoRII, cleavage by Polarity, suppression of protection against, 990 insertion mutations in gal operon and N muta- Repair deficiency tions in phage X, 1108 B. subtilis dna-8132, 1037 Polyangium cellulosum ultrastructure, 1278 Repellents ofBacillus subtilis Polysomal ribonucleic acid ofmeiotic yeast cells, 661 recognition sites, 72 Precursor of nitrate reductase Replication inhibitors E. coli, 122 recombination, effect on, 108 Prephenate dehydrogenase form Alcaligenes eutro- R factor R100 phus, 712, 723 plasmid co-integrates, 166 Propanediol as a carbon source R factor R.100.1 fucose pathway, disruption of, 1166 suppression of dnaA mutation, 1215 Prophage inducibility Rhizobiumjaponicum B. subtilis dna-8132, 1037 leucine and isoleucine-valine loci, 997 Prophage lambda Rhizoctonia leguminicola lysine catabolism, 338 plasmid co-integrates, 166 Rhizoctonia solani Prophage SP02 L-phenylalanine ammonia-lyase, regulation of, B. subtilis recC strains, recombination-deficient 568 phenotype of, 556 Rhodopseudomonas capsulata Prosthecae ofAsticcacaulis biprosthecum genes for carotenoid and bacteriochlorophyll bio- glucose transport, 282 synthesis, 619 Protein synthesis, barotolerant Rhodospirillum rubrum membranes, 1316, 1326 ion concentration, 654 Ribonucleic acid Proteus mirabilis meiotic cells of S. cerevisiae, 661 tryptophan operon, 679 nonribosomal nature ofnovel, stable species accu- Protocatechuate 3,4-dioxygenase isofunctional en- mulated by Aphanocapsa, 1020 zymes D-Ribose and L-rhamnose isomerization immunological properties, 516 catalyzed by the same enzyme in M. smegmatis, Protocatechuate pathway in Pseudomonas putida 553 mutants Ribosomal precursors ofBacillus megaterium, 1149 constitutive synthesis of enzymes, 272 Ribosomal protein cistrons Protonmotive force extragenic suppression, 563 E. coli, 1224 Ribulose 1,5-bisphosphate carboxylase S. aureus, 1224 E. halophila, 1271 Proton movement rif locus in Escherichia coli lactate and alanine transport in E. coli, 1232 ribosomal protein cistrons, extragenic suppres- VOL. 126, 1976 SUBJECT INDEX ix sion of, 563 heat activation of spores, 24 Rothia dentocariosa nutritional conditions for spore germination, 13 lactate dehydrogenase activity, 1344 Surface layers of Clostridium thermosaccharolyti- R-plasmid replication in Escherichia coli, 454 cum and Clostridium thermohydrosulfuricum, 377 Saccharomyces allophanate hydrolase activity, 198 Temperature effects on yield and maintenance Saccharomyces cerevisiae glucose-limited continuous culture ofE. coli, 251 inositol biosynthesis, control of, 232, 243 Thermal prophage inducibility nuclear staining method, 1339 B. subtilis dna-8132, 1037 RNA synthesized in meiotic cells, 661 Thiamine pyrophosphate synthesis Salmonella typhimurium M. denitrificans, 1030 aminoacyl-tRNA synthetases, regulation of, 634 Threonine catabolism hypoxanthine and guanine uptake in membrane E. coli, 1245 vesicles, 312 i-threonine dehydrogenase, 1245 siderophore iron transport, 823 Threonine deaminase tryptophan operon, 679 E. coli, 56 Selective methylation, incorrect hypothesis, 1009 E. coli nonsense mutant, 100 Separation of cell populations, 977 half-of-the-sites reactivity, 100 Septal complex in Filobasidiella neoformans (Cryp- Thymidine tococcus neoformans), 524 uptake and utilization in E. coli, 814 D-Serine deaminase ofEscherichia coli Torulopsis glabrata cleavage and aggregation, 132 circular DNA, 959 Shigella dysenteriae Transfer ribonucleic acid, undermethylated tryptophan operon, 668, 679 B. subtilis, 294 Sialidase and hemagglutinin from Clostridium per- Transloction in Escherichia coli fringens, 831, 845 DNA carrying amp, 1 Siderophore iron transport Tryptophan biosynthesis E. coli, 823 S. dysenteriae, 668 S. typhimurium, 823 Tryptophan operon Siderophore protection against colicins interspecific hybrids of enteric bacteria, 679 E. coli, 7 S. dysenteriae, 668 Slime mold Tryptophan synthase alkaline phosphatase activity, 1005 autogenous regulation in P. putida, 547 SmaR and EcoRI restriction Tumbling in chemotaxis *80dargF DNA, 487 divalent cation, control by, 706 Sodium dodecyl sulfate zebra structures, 1285 Ultraviolet-induced damage, repair of Sordaria brevicollis ascospores, 883 M. radiophilus, 587 Sphaerotilus discophorus Ultraviolet light iron deposition, 257, 264 AS-1 cyanophage in A. nidulans, inactivation of, Sporangia ofBacillus sphaericus 550 peptidoglycan synthetase activity, 213 Undermethylated transfer ribonucleic acid from SP02 prophage Bacillus subtilis B. subtilis recC strains, recombination-deficient construction and analysis, 294 phenotype of, 556 Spore coat protein Virus-induced deoxyribonuclease activity B. subtilis asporogenic mutants, 1342 A. nidulans, 630 Spore cortex precursor synthesis, site of B. sphaericus, 213 Spores Wall autolysis B. subtilis cell development, 384 inhibition by lipoteichoic acid and lipids, 192 S. viridochromogenes, 13, 24 S. faecalis, 192 Sporulation B. subtilis, 928 X-ray-induced repair-type deoxyribonucleic acid Staphylococcus aureus synthesis nitrate reductase activity in heme mutants, 225 E. coli, 785 protonmotive force, 1224 xthA locus in Escherichia coli, 1082 Streptococcus faecalis wall autolysis, inhibition of, 192 Yeast Streptococcus pyogenes L-form C27 sterol-accumulating strains, enzymatic analy- peptidoglycan fornation, 300 sis of, 1012 Streptococcus sanguis (Wicky) inositol biosynthesis, 232, 243 pH effect on competence development and DNA uptake, 205 Zebra structures Streptomyces viridochromogenes B. subtilis, 1285 INDEX TO DATE OF ISSUE

Month Date of Issue Pages

April 9 April 1976 1-561 May 10 May 1976 563-1023 June 7 June 1976 1025-1356 JOURNAL

BACTERIOLOGY

VOLUME 126

WASHINGTON, D.C. 20006

1976 JOURNAL OF BACTERIOLOGY

VOLUME 126 * 1976 EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1980) University of Delaware, Newark

S. G. BRADLEY, Editor (1979) ALLEN T. PHILLIPS, Editor (1980) Virginia Commonwealth University, Richmond Pennsylvania State University, University Park, Pa. ROY CURTISS HI, Editor (1979) CARL SCHNAITMAN, Editor (1978) University of Alabama, Birmingham University of Virginia, Charlottesville ELIZABETH McFALL, Editor (1980) H. E. UMBARGER, Editor (1976) New York University, New York, N.Y. Purdue University, Lafayette, Ind.

James N. Adams (1976) Walton L. Fangman (1978) Eugene W. Nester (1978) Arthur I. Aronson (1976) Gerald R. Fink (1978) Donald P. Nierlich (1977) Gad Avigad (1977) W. R. Finnerty (1976) Michio Oishi (1977) Stephen D. Barbour (1976) Walter R. Guild (1978) Ronald H. Olsen (1978) Blaine Beaman (1976) George Hegeman (1977) Charles Panos (1978) Claire M. Berg (1976) James A. Hoch (1977) Leo Parks (1976) Richard S. Berk (1977) Joy Hochstadt (1977) Martin Pato (1978) Hardet Bernheimer (1977) Stanley C. Holt (1977) Paul V. Phibbs (1976) Judith S. Bond (1977) Joseph Inselburg (1978) Charles C. Remsen IU (1976) Winfried Boos (1976) Sam Kaplan (1977) William S. Reznikoff (1976) Jean Brenchley (1977) Jost Kemper (1978) Palmer Rogers (1978) George H. Brownell (1977) David E. Kennel (1977) Antonio H. Romano (1976) Richard 0. Burns (1977) Bruce C. Kline (1977) Robert Rownd (1977) Donald B. Clewell (1977) Robert L. Lester (1978) Harold L. Sadoff (1977) Stanley N. Cohen (1976) Hillel S. Levinson (1978) Irwin H. Segel (1976) Ronald S. Cole (1977) James S. Lovett (1977) Jane K. Setlow (1978) S. F. Conti (1978) K. Brooks Low (1976) Stanley K. Shapiro (1976) Stephen Cooper (1978) Calvin S. McLaughlin (1978) Simon Silver (1976) John E. Cronan, Jr. (1977) P. T. Magee (1977) Lucile Smith (1978) Walter J. Dobrogosz (1976) Michael H. Malamy (1978) James A. Wechsler (1978) Patrick R. Dugan (1977) Manley Mandel (1978) David White (1977) Alan D. Elbein (1976) Robert E. Marquis (1976) Clifford A. Woolfolk (1976) Wolfgang Epstein (1978) Neil H. Mendelson (1978) Howard Zalkin (1976) David P. Fan (1978) Gene E. Michaels (1977)

Robert A. Day, Managing Editor Gisella Pollock, Director of Editorial Services Linda Illig, Production Editor 1913 I St., N. W., Washington, D. C. 20006 EX OFFICIO Helen R. Whiteley, President (1975-1976) Harlyn 0. Halvorson, Vice-President (1975-1976) J. Mehsen Joseph, Secretary Brinton M. Miller, Treasurer

AND P. A. LEMKE ...... 417 Ultrastructure of Septal Complex in Filobasidiella neoformans (Cryptococcus neoformans). K. J. KWON-CHUNG* AND TERRY J. POPKIN ...... 524 Genetics and Molecular Biology Translocation of a Discrete Piece of Deoxyribonucleic Acid Carrying an amp Gene Between Replicons in Escherichia coli. P. M. BENNETT* AND M. H. RICHMOND ...... 1 Genetics and Complementation of Haemophilus influenzae Mutants Deficient in Adenosine 5'-Triphosphate-Dependent Nuclease. JAN KOOISTRA, GARY D. SMALL, JANE K. SETLOW,* AND ROSLYN SHAPANKA ...... 31 Method for the Isolation ofEscherichia coli K-12 Mutants Deficient in Essential Genes. KAREN A. ARMSTRONG* AND ROBERT K. HERMAN ...... 38 Essential Genes in the metB-malB Region of Escherichia coli K-12. KAREN A.

ARMSTRONG* AND DAVID F. FAN ...... 48 Threonine Deaminase from Escherichia coli: Feedback-Hypersensitive Enzyme from a Genetic Regulatory Mutant. DAVID H. CALHOUN ...... 56 Comparative Studies on Membrane-Associated, Folded Chromosomes from Esche- richia coli. PAUL DWORSKY ...... 64 Mapping of Two Loci Affecting the Regulation of Branched-Chain Amino Acid Transport in Escherichia coli K-12. JAMES J. ANDERSON, STEVEN C. QUAY, AND DALE L. OXENDER* ...... 80 Effect of Deoxyribonucleic Acid Replication Inhibitors on Bacterial Recombina- tion. UMBERTO CANOSI,* ANTONIO G. SICCARDI, ARTURO FALASCHI, AND GIORGIO MAZZA ...... 108 Escherichia coli Mutants Altered in Murein Lipoprotein. HENRY C. WU* AND JIM JUNG-CHING LIN ...... 147 Evidence for Diverse Types of Large Plasmids in Tumor-Inducing Strains of Agrobacterium. T. C. CURRIER AND E. W. NESTER* ...... 157 Plasmid Co-Integrates of Prophage Lambda and R Factor R100. WALTER B. DEMPSEY* AND NEIL S. WILLETTS ...... 166 Isolation of Nonsense Suppressor Mutants in Pseudomonas. LEONARD MIN- DICH,* JEFFREY COHEN, AND MARTIN WEISBURD ...... 177

* Asterisk refers to person to whom inquiries regarding the paper should be addressed. xiii XiV CONTENTS Effect of pH on Competence Development and Deoxyribonucleic Acid Uptake in Streptococcus sanguis (Wicky). JON M. RANHAND ...... 205 Temperature Dependence of Mating-Pair Formation in Escherichia coli. ROGER H. WALMSLEY ...... 222 Constitutive Synthesis of Enzymes of the Protocatechuate Pathway and of the ,8-Ketoadipate Uptake System in Mutant Strains of Pseudomonas putida. DONNA PARKE* AND L. NICHOLAs ORNSTON ...... 272 Undermethylated Transfer Ribonucleic Acid from a Relaxed Strain of Bacillus subtilis: Construction of the Strain and Analysis of the Transfer Ribonucleic Acid. NIcoLE KEISEL AND BARBARA VOLD* ...... 294 Dual Regulation by Arginine of the Expression of the Escherichia coli argECBH Operon. RICHARD A. KRZYZEK AND PALMER ROGERS* ...... 348 Effect of Arginine on the Stability and Size of argECBH Messenger Ribonucleic Acid in Escherichia coli. RICHARD A. KRZYZEK AND PALMER ROGERS*.. 365 Cell Division in Pseudomonas aeruginosa: Participation of Alkaline Phospha- tase. A. R. BHATTI, I. W. DEVOE, AND J. M. INGRAM* ...... 400 Isolation of Plasmid Deoxyribonucleic Acid from Pseudomonas putida. SUNIL PALCHAUDHURI* AND A. CHAKRABARTY ...... 410 Molecular Nature of Two Beta-Lactamase-Specifying Plasmids Isolated from Haemophilus influenzae Type b. JOHANNES DE GRAAFF, LYNN P. ELWELL,* AND STANLEY 'FALKOW ...... - 439 Characterization of a Mini-ColEl Plasmid. VICKERS HERSHFIELD, HERBERT W. BOYER, L. CHOW, AND DONALD R. HELINSKI* ...... 447 Mode of Replication of the Conjugative R-Plasmid RSF1040 in Escherichia coli. JORGE H. CROSA,* LINDA K. LurTTopp, AND STANLEY FALKOW ...... 454 Isolation and Characterization of the Specialized Transducing Bacteriophages .80dargF and Xh8OcI857dargF: Specific Cleavage of Arginine-Transducing Deoxyribonucleic Acid by the Endonucleases EcoRI and SmaR. PHILIP M. JAMES, DON SENS, WILLIAM NATTER, STEVEN K. MOORE, AND ERIC JAMES* . 487 Isolation and Genetic Analysis of Amber uvrA and uvrB Mutants. MITSUOKI MORIMYO,* YOSHIE SHIMAZU, AND NAOAKI ISHII ...... 529 New Purine Markers in Bacillus subtilis. M. E. LOPEz, F. A. FERRARI, A. G. SICCARDI,* G. MAZZA, AND M. POLSINELLI ...... 533 Methyl Galactosidase Activity: an Alternative Evolutionary Destination for the ebgAo Gene. BARRY G. HALL...... 536 Evidence for Autogenous Regulation of Pseudomonas putida Tryptophan Synthase. ALAN R. PROCTOR AND IRVING P. CRAWFORD* ...... 547 Ultraviolet Light Inactivation and Photoreactivation of AS-i Cyanophage in Anacystis nidulans. YUKIo ASATO...... 550 Association of the Recombination-Deficient Phenotype of Bacillus subtilis recC Strains with the Presence of an SP02 Prophage. ANTHONY J. GARRO,* CAROLE SPROUSE, AND JAMES G. WETMUR...... 556 Simplified Method for Interruption of Conjugation in Escherichia coli. DAVID ZIPKAS AND MONICA RILEY* ...... 559 Physiology and Metabolism Siderophore Protection Against Colicins M, B, V, and Ia in Escherichia coli. RUSH WAYNE, KEVIN FRICK, AND J. B. NEILANDS*...... 7 Nutritionally Defined Conditions for Germination of Streptomyces viridochromogenes Spores. C. F. HIRSCH AND J. C. ENSIGN* ...... 13 Heat Activation of Streptomyces viridochromogenes Spores. C. F. HIRSCH AND J. C. ENSIGN* ...... 24 CONTENTS xv Biosynthesis of Membrane-Bound Nitrate Reductase in Escherichia coli: Evidence for a Soluble Precursor. C. H. MAcGREGOR ...... 122 Specific In Vivo Cleavage of D-Serine Deaminase and Properties of Tetrameric Polypeptide Aggregates of the Fragments. MARIA C. HEINCZ AND ELIZABETH MCFALL* ...... 132 Inactivation ofMembrane Transport inEscherichia coli by Near-Ultraviolet Light. ARTHUR L. KOCH, R. J. DOYLE, AND H. E. KUBITSCHEK* ...... 140 Puromycin-Resistant Biosynthesis of a Specific Outer-Membrane Lipoprotein of Escherichia coli. SIMON HALEGOUA, AiuKAzu HIRASHIMA, AND MASAYORI INOUYE* ...... 183 Inhibition of Wall Autolysis in Streptococcus faecalis by Lipoteichoic Acid and Lipids. R. F. CLEVELAND,* A. J. WICKEN, L. DANEO-MOORE, AND G. D. SHOCKMAN ...... 192 Sequence of Molecular Events Involved in Induction of Allophanate Hydrolase. JUNE BOSSINGER AND TERRANCE G. COOPER*...... 198 Nitrate Reductase Activity in Heme-Deficient Mutants of Staphylococcus aureus. KATHLEEN A. BURKE AND JUNE LASCELLES* ...... 225 Control of Inositol Biosynthesis in Saccharomyces cerevisiae: Properties of a Re- pressible Enzyme System in Extracts of Wild-Type (Ino+) Cells. MICHAEL R. CULBERTSON, THOMAS F. DONAHUE, AND SUSAN A. HENRY* ...... 232 Control of Inositol Biosynthesis in Saccharomyces cerevisiae: Inositol-Phosphate Synthetase Mutants. MICHAEL R. CULBERTSON, THOMAS F. DONAHUE, AND SUSAN A. HENRY* ...... 243 Effects of Growth Temperature on Yield and Maintenance During Glucose- Limited Continuous Culture of Escherichia coli. STANLEY E. MAINZER AND WALTER P. HEMPFLING* ...... 251 Measurement of Growth and Iron Deposition in Sphaerotilus discophorus. STEPHEN R. ROGERS* AND JAMES J. ANDERSON ...... 257 Role of Iron Deposition in Sphaerotilus discophorus. STEPHEN R. ROGERS* AND JAMES J. ANDERSON ...... 264 Glucose Transport in Isolated Prosthecae ofAsticcacaulis biprosthecum. ROBERT J. LARSON AND JACK L. PATE* ...... 282 Defective Synthesis of Lipid Intermediates for Peptidoglycan Formation in a Stabilized L-Form of Streptococcus pyogenes. VICTOR M. REUSCH, JR., AND CHARLES PANOS* ...... 300 Regulation of Purine Utilization in Bacteria. VI. Characterization of Hypo- xanthine and Guanine Uptake into Isolated Membrane Vesicles from Salmo- nella typhimurium. LANCE E. JACKMAN AND Joy HOCHSTADT* ...... 312 Protonmotive Force as the Source of Energy for Adenosine 5'-Triphosphate Syn- thesis in Escherichia coli. DOROTHY M. WUSON,* JOHN F. ALDERETE, PETER C. MALONEY, AND T. HASTINGS WILSON ...... 327 Lysine Catabolism in Rhizoctonia leguminicola and Related Fungi. F. PETER GUENGERICH AND HARRY P. BROQUIST* ...... 338 Mode of Insertion of Lipopolysaccharide into the Outer Membrane of Escherichia coli. CHARLES F. KULPA, JR., AND LORETTA LEIVE* ...... 467 Formation of the Formate-Nitrate Electron Transport Pathway from Inactive Components in Escherichia coli. RANDOLPH H. SCorr AND JOHN A. DEMOsS* 478 Role of Arginine Deiminase in Growth ofMycoplasma hominis. JANE D. FENSKE AND GEORGE E. KENNY* ...... 501 Relationship of Structure to Function in Bacterial Endotoxins: Differences in the Lipid Moiety of Endotoxic Glycolipids. AH-KAU NG, R. CHRISTOPHER BUTLER, CHEN-LO H. CHEN, AND ALOIs NOWOTNY* ...... 511 XVi CONTENTS Characteristics of Bacillus stearothermophilus Mutants Blocked in Catabolic Function. J. J. ROWE, I. D. GOLDBERG, AND R. E. AMELUNXEN* ...... 520 Cyanide-Resistant Respiration of a Neurospora crassa Membrane Mutant. DAVOR JURETIC...... 542 Formation of Indole-3-Carboxylic Acid by Chromobacterium violaceum. PATRICK J. DAVIS, MARK E. GUSTAFSON, AND JOHN P. RoSAZZA* ...... 544 Enzymology Threonine Deaminase from a Nonsense Mutant of Escherichia coli Requiring Isoleucine or Pyridoxine: Evidence for Half-of-the-Sites Reactivity. JtCRGEN FELDNER AND HERBERT GRIMMINGER* ...... 100 Purification and Properties of a Manganese-Stimulated Endonuclease from Bacillus subtilis. B. SCHER AND D. DUBNAU* ...... 429 Immunological Properties of Protocatechuate 3,4-Dioxygenase Isofunctional Enzymes. CHING-TSANG Hou* AND MARJORIE O. LILLARD ...... 516 Active Subunits ofEscherichia coli Glutamate Synthase. PEKKA MANTSALA AND HOWARD ZALKIN* ...... 539 Evidence that the Isomerization of D-Ribose and L-Rhamnose Is Catalyzed by the Same Enzyme in Mycobacterium smegmatis. KEN IZUMORI, MIKE MITCHELL, AND ALAN D. ELBEIN* ...... 553 Erratum Galactosaminogalactan from Cell Walls of Aspergillus niger. PROMODE C. BARDALAYE AND JOHN H. NORDIN* ...... 561

Volume 126 Contents for May Number 2 Morphology and Ultrastructure Germ Tube Formation from Zonal Rotor Fractions of Candida albicans. W. LAJEAN CHAFFIN* AND STEPHEN J. SOGIN ...... 771 Ultrastructure of the Cell Walls of Two Closely Related Clostridia That Possess Different Regular Arrays of Surface Subunits. UwE B. SLEYTR* AND AUDREY M. GLAUERT ...... 869 Ultrastructure of Developing Ascospores in Sordaria brevicollis. CHARLES J. HACKETT AND KUO-CHUN CHEN* ...... 883 Relationship Between Cortex Content and Properties of Bacillus sphaericus Spores. YASUO IMAE AND JACK L. STROMINGER* ...... 907 Cortex Content of Asporogenous Mutants of Bacillus subtilis. YASUO IMAE AND JACK L. STROMINGER* ...... 914 Development of Microbodies in the Yeast Kloeckera Growing on Methanol. ATSuo TANAKA, SHIGEKI YASUHARA, SusuMu KAWAMOTO, SABURO FUKUI,* AND MASAKO OSUMI ...... 919 Genetics and Molecular Biology Extragenic Suppression of Two Ribosomal Protein Cistrons Lying near the rif Locus in Escherichia coli. BRUCE MOLHOLT ...... 563 Role of a Lipopolysaccharide Gene for Immunogenicity of the Enterobacterial Common Antigen. G. SCHMIDT,* D. MANNEL, H. MAYER, H. Y. WHANG, AND E. NETER ...... 579 Repair of Ultraviolet Light-Induced Damage in Micrococcus radiophilus, an CONTENTS * ai Extremely Resistant Microorganism. MARTIN F. LAVIN, ARTHUR JENKINS, AND CHEV KIDSON* ...... 587 Characterization of an Escherichia coli K-12 F-Con- Mutant. Louis M. HAVEKES AND WIEL P. M. HOEKSTRA* ...... 593 Differences in the Genetic Structure of Bacillus subtilis Strains Carrying the trpE26 Mutation and Strain 168. J. TROWSDALE AND C. ANAGNOSTOPOULOS* 609 Map of Genes for Carotenoid and Bacteriochlorophyll Biosynthesis in Rhodopseu- domonas capsulata. HUEI-CHE YEN AND BARRY MARRS* ...... 619 Formation, in the Dark, of Virus-Induced Deoxyribonuclease Activity inAnacystis nidulans, an Obligate Photoautotroph. J. UDVARDY, B. SIV6K, G. BORBELY, AND G. L. FARKAS* ...... 630 Growth Rate Modulation of Four Aminoacyl-Transfer Ribonucleic Acid Synthe- tases in Enteric Bacteria. W. GALEN MCKEEVER AND FREDERICK C. NEIDHARDT* ...... 634 Methyl Methane Sulfonate-Sensitive Mutant of Escherichia coli Deficient in an Endonuclease Specific for Apurinic Sites in Deoxyribonucleic Acid. S. LJUNGQUIST, T. LINDAHL,* AND P. HOWARD-FLANDERS ...... 646 Naturally Occurring Sites Within the Shigella dysenteriae Tryptophan Operon Severely Limit Tryptophan Biosynthesis. MICHAEL D. MANSON AND CHARLES YANOFSKY*...... 668 Tryptophan Operon Regulation in Interspecific Hybrids of Enteric Bacteria. MICHAEL D. MANSON AND CHARLES YANOFSKY*...... 679 An Enzyme Common to Histidine and Aromatic Amino Acid Biosynthesis in Bacillus subtilis. EUGENE W. NESTER* AND ALICE L. MONTOYA ...... 699 cheA, cheB, and cheC Genes of Escherichia coli and Their Role in Chemotaxis. JOHN S. PARKINSON ...... 758 Role of Deoxyribonucleic Acid Ligase in a Deoxyribonucleic Acid Membrane Frac- tion Extracted from Pneumococci. MILFORD GREENE AND WILLIAM FIRSHEIN* 777 Role of Deoxyribonucleic Acid Polymerases and Deoxyribonucleic Acid Ligase in X-Ray-Induced Repair Synthesis in Toluene-Treated Escherichia coli K-12. D. BILLEN* AND G. R. HELLERMANN ...... -785 Plasmid-Determined Alcohol Dehydrogenase Activity in Alkane-Utilizing Strains of Pseudomonas putida. SPENCER BENSON AND JAMES SHAPIRO* ...... 794 Arrangement and Regulation of the Nitrogen Fixation Genes in Klebsiella pneumoniae Studied by Derepression Kinetics. ALAN COLLMER AND MARVIN LAMBORG* ...... 806 Clo DF13 Plasmid Deoxyribonucleic Acid-Directed In Vitro Synthesis of Biologi- cally Active Cloacin DF13 and Clo DF13 Immunity Protein. RUUD N. H. KONINGS,* PETER M. ANDREOLI, EDUARD VELTKAMP, AND H. JOHN J. NIJKAMP 861 Initial Characterization of Aspergillus nidulans Mutants Blocked in the Nuclear Replication Cycle. E. ORR AND R. F. ROSENBERGER*...... 895 Determination of the Execution Points of Mutationsin the Nuclear Replication Cycle ofAspergillus nidulans. E. ORR AND R. F. ROSENBERGER* ...... 903 Respiratory-Deficient Mutants of Torulopsis glabrata, a Yeast with Circular Mito- chondrial Deoxyribonucleic Acid of 6 am. R. M. O'CONNOR, C. R. McARTHUR, AND G. D. CLARK-WALKER*...... 959 Centrifugal Separation of Irradiated Cultures ofEscherichia coli Cells into Viable and Nonviable Populations. R. L. SCHENLEY,* W. D. FISHER, P. A. SWENSON, AND G. G. KHACHATOURIANS ...... 977 Pyrimidine Dimer Excision in Surviving and Nonsurviving Cells of Ultraviolet- Irradiated Cultures ofEscherichia coli. R. L. SCHENLEY,* W. D. FISHER, AND P. A. SWENSON...... 985 xviii CONTENTS In Vivo Methylation by Escherichia coli K-12 mec+ Deoxyribonucleic Acid-Cytosine Methylase Protects Against In Vitro Cleavage by the RII Restriction Endo- nuclease (R EcoRII). SAMUEL SCHLAGMAN, STANLEY HATTMAN,* MAUREEN S. MAY, AND LISBETH BERGER ...... 990 Genetic Mapping of Leucine and Isoleucine-Valine Loci in Rhizobiumjaponicum. FRENY DOCTOR AND V. V. MODI* ...... 997 Altered Deoxyribonucleotide Pools in P2 Eductants ofEscherichia coli K-12 due to Deletion of the dcd Gene. JAN NEUHARD* AND ELISABETH THOMASSEN .... 999 Selective Methylation: an Incorrect Hypothesis. LEE SHUGART ...... 1009 Hypersensitivity to Catabolite Repression in the L-Arabinose Operon of Esche- richia coli B/r Is trans Acting. DAVID E. SHEPPARD* AND MARIANNE ELEUTERIO ...... 1014 Nonribosomal Nature of Novel, Stable Ribonucleic Acid Species Accumulated by Blue-Green Bacteria. L. BONEN, G. V. ALLEN, P. R. DOBSON, AND W. F. DOOLITTLE* ...... 1020 Physiology and Metabolism Regulation of L-Phenylalanine Ammonia-Lyase from Rhizoctonia solani. KRISHNA K. KALGHATGI AND P. V. SUBBA RAO* ...... 568 Effect of Colicin K on a Membrane-Associated, Energy-Linked Function. SOHAIR F. SABET ...... 601 Specific Ion Concentration as a Factor in Barotolerant Protein Synthesis in Bac- teria. WALTER P. SMITH, J. V. LANDAU,* AND D. H. POPE ...... 654 Ribonucleic Acid Synthesized in Meiotic Cells of Saccharomyces cerevisiae: Effect of Culture Medium pH. MICHAEL S. CURIALE, MARTA M. PETRYNA, AND DALLICE MILLS* ...... 661 Influence of Exogenous Sugars and Polyols on Cl- Influx and Efflux by the Ascomycete Neocosmospora vasinfecta. A. G. MILLER* AND K. BUDD ...... 690 Control of Tumbling in Bacterial Chemotaxis by Divalent Cation. GEORGE W. ORDAL ...... 706 Ammonia-Sensitive Mutant of Klebsiella aerogenes. KEVIN STRUHL AND BORIS MAGASANIK* ...... 739 Fatty Acid Transport by the Lipophilic Bacterium Nocardia asteroides. ROBERT CALMES* AND S. J. DEAL ...... 751 Biochemical and Genetic Studies on Galactosamine Metabolism in Neurospora crassa. CLARK M. EDSON AND STUART BRODY* ...... 799 Thymidine Uptake and Utilization in Escherichia coli: a New Gene Controlling Nucleoside Transport. MICHAEL McKEOWN, MICHAEL KAHN, AND PHILIP HANAWALT* ...... 814 Mechanisms of Siderophore Iron Transport in Enteric Bacteria. JOHN LEONG AND J. B. NEILANDS* ...... 823 Relationship Between Hemagglutinin and Sialidase from Clostridium perfringens CN3870: Chromatographic Characterization of the Biologically Active Pro- teins. JULIAN I. ROOD* AND RUSSELL G. WILKINSON ...... 831 Relationship Between Hemagglutinin and Sialidase from Clostridium perfringens CN3870: Gel Filtration of Mutant and Revertant Activities. JULIAN I. ROOD* AND RUSSELL G. WILaINSON ...... 845 Lack of Glucose Phosphotransferase Function in Phosphofructokinase Mutants of Escherichia coli. R. A. ROEHL AND R. T. VINOPAL* ...... 852 Use of Temperature-Sensitive Mutants to Study Gene Expression During Sporula- tion in Bacillus subtilis. M. YOUNG ...... 928 CONTENTS XiX Phosphatases of Chlamydomonas reinhardi: Biochemical and Cytochemical Ap- proach with Specific Mutants. R. F. MATAGNE,* R. LOPPES, AND R. DELTOUR 937 Periplasmic Protein Related to the sn-Glycerol-3-Phosphate Transport System of Escherichia coli. T. J. SILHAVY, I. HARTIG-BEECKEN, AND W. BOOS* ...... 951 Autolysis of Neisseria gonorrhoeae. THEODOR ELMROS,* LARS G. BURMAN, AND GUNNAR D. BLOOM ...... 969 Effect of Ammonium Ions on the Induction of Nitrite Reductase in Neurospora crassa. H. C. P. CHANG AND G. J. SORGER* ...... 1002 Dissociation of Developing Slime Mold Cells Does Not Inhibit the Developmen- tally Regulated Rise in Alkaline Phosphatase Activity. VLADIMIR ATRYZEK 1005 Inhibition of Dimethyl Ether and Methane Oxidation in Methylococcus capsulatus and Methylosinus trichosporium. RAMESH PATEL,* C. T. Hou, AND A. FELIX 1017 Enzymology Purification and Properties of Chorismate Mutase-Prephenate Dehydratase and Prephenate Dehydrogenase from Alcaligenes eutrophus. BARBEL FRIED- RICH,* CORNELIUS G. FRIEDRICH, AND HANS G. SCHLEGEL ...... 712 Regulation of Chorismate Mutase-Prephenate Dehydratase and Prephenate Dehydrogenase from Alcaligenes eutrophus. CORNELIUS G. FRIEDRICH, BARBEL FRIEDRICH,* HANS G. SCHLEGEL ...... 723 Product Analysis of Bisulfite Reductase Activity Isolated from Desulfovibrio vulgaris. H. L. DRAKE AND J. M. AKAGI* ...... 733 Kinetic Studies on Bacillus polymyxa Nitrogenase. T. E. HERMANN* AND P. W. WILSON ...... 743 Enzymatic Analysis of C27 Sterol-Accumulating Yeast Strains. R. B. BAILEY, LYNN MILLER, AND L. W. PARKS* ...... 1012 Volume 126 Contents for June Number 3 Morphology and Ultrastructure Volatile Factor Involved in the Dimorphism of Mucor racemosus. DUANE T. MOONEY AND PAUL S. SYPHERD* ...... 1266 Ultrastructure of Polyangium cellulosum. J. R. LAMPKY ...... 1278 Cellular Organization of Bacillus subtilis: Sodium Dodecyl Sulfate-Induced Cell Partitioning into Zebra Structures. NEIL H. MENDELSON,* STEVEN M. HAAG, AND ROGER M. COLE ...... 1285 Rapid Nuclear Staining Method for Saccharomyces 'cerevisiae. MARTIN L. SLATER ...... 1339 Genetics and Molecular Biology Mutant of Escherichia coli That Instantaneously Loses the Ability to Adsorb Lambda Bacteriophage upon Exposure to High Temperature. TAKAO SHINOZAWA* AND HISATOSHI SHIDA ...... 1025 Repair Deficiency, Mutator Activity, and Thermal Prophage Inducibility in dna- 8132 Strains ofBacillus subtilis. YOSHITO SADAIE* AND KIKUKO NARUI .... 1037 Amino Acid Starvation in Escherichia coli K-12: Characteristics of the Translation Process. C. S. SUBRAHMANYAM AND H. K. DAS* ...... 1042 Genetic and Biochemical Characterization of Some Missense Mutations in the lacZ Gene of Escherichia coli K-12. PENELOPE TRUMAN AND P. L. BERGQUIST* ...... 1063 Genetic Mapping ofxthA, the Structural Gene for Exonuclease III in Escherichia xx CONTENTS coli K-12. BRIAN J. WHITE, STEVEN J. HOCHHAUSER, NITZA M. CINTR6N, AND BERNARD WEISS*...... 1082 Mutants in Three Genes Affecting Transport of Magnesium in Escherichia coli: Genetics and Physiology. MYUNG HEE PARK, BRENDA B. WONG, AND JOAN E. LUSK* ...... 1096 Inheritance of Nocardiophage 4EC in Matings of Nocardial Lysogens. GEORGE H. BROWNELL* AND JAMES N. ADAMS ...... 1104 Suppression of Polarity of Insertion Mutations in the gal Operon and N Muta- tions in Bacteriophage Lambda. OSCAR REYES, MAX GOTTESMAN, AND SANKAR ADHYA*...... 1108 Calcium-Requiring Step in the Uptake of Deoxyribonucleic Acid Molecules Through the Surface of Competent Pneumococci. HIROYUKI SETO AND ALEXANDER TOMASZ* ...... 1113 Mutations Affecting Catabolite Repression of the L-Arabinose Regulon in Escherichia coli B/r. LAUREL HEFFERNAN,* RICHARD BASS, AND ELLIS ENGLESBERG...... 1119 Effect of araC Gene Product on Catabolite Repression in the L-Arabinose Regulon. LAUREL HEFFERNAN* AND GARY WILCOX...... 1132 Escherichia coli Mutants Deficient in Guanine-Xanthine Phosphoribosyltransfer- ase. JOSEPH A. HOLDEN, PHILIP D. HARRIMAN,* AND JUDY D. WALL...... 1141 Ribosomal Precursor Particles of Bacillus megaterium. BARBARA A. BODY AND BERNARD H. BROWNSTEIN* ...... 1149 Isolation and Characterization of Conjugation-Deficient Mutants of Escherichia coli K-12. JOSEPH 0. FALKINHAMIII* AND RoY CURTIS III...... 1194 Suppression of an Escherichia coli dnaA Mutation by the Integrated R Factor R.100.1: Change of Chromosome Replication Origin in Synchronized Cultures. ROBERT E. BIRD, MICHAEL CHANDLER,* AND LUCIEN CARO...... 1215 Electrophoretic Comparison of Endonuclease-Digested Plasmids from Neisseria gonorrhoeae. R. SCOTT FOSTER* AND GAYLE C. FOSTER ...... 1297 Structure of Caulobacter Deoxyribonucleic Acid. NANCY B. WOOD, ADRIAN V. RAKE, AND LUCILLE SHAPIRO*...... 1305 Cross-Resistance Between Bacteriophages and Colicins in Escherichia coli K-12. ROBERT E. W. HANCOCK, JOHN K. DAVIES,* AND PETER REEVES...... 1347 Partial Characterization of a Temperature-Sensitive Mutation Affecting Acetyl Coenzyme A Carboxylase in Escherichia coli K-12. DAVID F. SILBERT,* TIMOTHY POHLMAN, AND ANN CHAPMAN...... 1351 Physiology and Metabolism Pathway of Thiamine Pyrophosphate Synthesis in Micrococcus denitrificans. HIROSHI SANEMORI, YOSHIKo EGI, AND TAKASHI KAWASAKI* ...... 1030 Iron Uptake in Colicin B-Resistant Mutants of Escherichia coli K-12. ANTHONY P. PUGSLEY* AND PETER REEVES ...... 1052 Variation in Levels of Enzymes Related to Energy Metabolism in Alternative Developmental Pathways ofBlastocladiella emersonii. OLE C. INGEBRETSEN AND TORE SANNER* ...... 1075 Metal Ion Content of Escherichia coli Versus Cell Age. FANG-CHIEN KUNG, JONATHAN RAYMOND, AND DONALD A. GLASER* ...... 1089 Incorporation of Deoxycytidine into Deoxyribonucleic Acid Deoxycytidylate in Lactobacillus acidophilus R-26. MINH-TAM B. DAVIS AND DAVID H. IVES*.. 1136 Properties of the Entry and Exit Reactions of the Beta-Methyl Galactoside Trans- port System in Escherichia coli. DAVID B. WILSON ...... 1156 Disruption of the Fucose Pathway as a Consequence of Genetic Adaptation to Propanediol as a Carbon Source in Escherichia coli. ANDREW J. HACKING CONTENTS XXi

AND E. C. C. LIN* ...... 1166 Compartmentation and Control of Arginine Metabolism in Neurospora. RICHARD L. WEISS ...... 1173 Chemical Composition and Turnover of Peptidoglycan in Neisseria gonorrhoeae. BRUCE H. HEBELER AND FRANK E. YOUNG* ...... 1180 Mechanism of Autolysis of Neisseria gonorrhoeae. BRUCE H. HEBELER AND FRANK E. YOUNG* ...... 1186 Carnitine Biosynthesis in Neurospora crassa: Enzymatic Conversion of Lysine to E-N-Trimethyllysine. CHARLES J. REBOUCHE AND HARRY P. BROQUIST* .... 1207 Magnitude of the Protonmotive Force in Respiring Staphylococcus aureus and Escherichia coli. STEPHEN H. COLLINS AND W. ALLAN HAMILTON* ...... 1224 Proton Movements Coupled to Lactate and Alanine Transport in Escherichia coli: Isolation of Mutants with Altered Stoichiometry in Alanine Transport. STEPHEN H. COLLINS, AUDREY W. JARVIS, RAYMOND J. LINDSAY, AND W. ALLAN HAMILTON* ...... 1232 Role Of L-Threonine Dehydrogenase in the Catabolism of Threonine and Synthe- sis of Glycine by Escherichia coli. E. B. NEWMAN,* V. KAPOOR, AND R. POTTER ...... 1245 Activity ofThree Murein Hydrolases During the Cell Division Cycle of Escherichia coli K-12 as Measured in Toluene-Treated Cells. BARBARA BECK AND JAMES T. PARK* ...... 1250 Membranes ofRhodospirillum rubrum: Isolation and Physicochemical Properties of Membranes from Aerobically Grown Cells. MARY LYNNE PERILLE COLLINS AND ROBERT A. NIEDERMAN* ...... 1316 Membranes of Rhodospirillum rubrum: Physicochemical Properties of Chro- matophore Fractions Isolated from Osmotically and Mechanically Disrupted Cells. MARY LYNNE PERILLE COLLINS AND ROBERT A. NIEDERMAN* ...... 1326 Appearance of Spore Coat Protein in the Cell Extracts ofBacillus subtilis Asporo- genic Mutants. ARITSUNE UCHIDA,* HAJIME KADOTA, AND PIERRE SCHAEFFER ...... 1342 Enzymology Purification and Properties of Nitroalkane-Oxidizing Enzyme from Hansenula mrakii. TOSHIKO KIDO, TATSUO YAMAMOTO, AND KENJI SODA* ...... 1261 Molecular and Catalytic Properties of Ribulose 1,5-Bisphosphate Carboxylase from the Photosynthetic Extreme Halphile Ectothiorhodospira halophila. F. ROBERT TABITA AND BRUCE A. MCFADDEN* ...... 1271 Regulation of Lactate Dehydrogenase Activity in Rothia dentocariosa by Fructose 1,6-Diphosphate and Adenosine 5'-Triphosphate. ROSELYN J. EISENBERG,* MARY ELCHISAK, AND JOANNE RUDD ...... 1344 Errata Analytical Isoelectric Focusing of R Factor-Determined f3-Lactamases: Correlation with Plasmid Compatibility. MARGARET MATTHEW* AND R. W. HEDGES ... 1355 Adaptation of a Stable L-Form of Bacillus subtilis to Minimal Salts Medium Without Osmotic Stabilizers. RICHARD W. GILPIN* AND SUZANNE K. PATTERSON ...... 1355 Inhibition of Wall Autolysis in Streptococcus faecalis by Lipoteichoic Acid and Lipids. R. F. CLEVELAND,* A. J. WICKEN, L. DANEO-MOORE, AND G. D. SHOCKMAN ...... 1355 Identification of Coreplicating Chromosomal Sectors in Bacillus subtilis by Nitro- soguanidine-Induced Comutation. ANTONIo G. SICCARDI, FRANCO A. FERRARI, GIORGIO MAZZA, AND ALESSANDRO GALIZZI* ...... 1356