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BAT2 Shrna (H) Lentiviral Particles: Sc-72612-V

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BAT2 Shrna (H) Lentiviral Particles: Sc-72612-V SANTA CRUZ BIOTECHNOLOGY, INC. BAT2 shRNA (h) Lentiviral Particles: sc-72612-V BACKGROUND APPLICATIONS BAT2, also known as G2, is a 2,157 amino acid protein that localizes to both BAT2 shRNA (h) Lentiviral Particles is recommended for the inhibition of the nucleus and the cytoplasm. Expressed in cell lines of leukemic origin, BAT2 expression in human cells. BAT2 exists as multiple alternatively spliced isoforms and is thought to play a role in the regulation of pre-mRNA splicing. The BAT2 gene maps within a SUPPORT REAGENTS cluster of BAT genes on human chromosome 6 and is implicated in the devel- Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen opment of rheumatoid arthritis and Insulin-dependent diabetes mellitus viral stock containing 1.0 x 106 infectious units of virus (IFU); contains an (IDDM). Chromosome 6, on which the BAT2 gene is localized, contains 170 shRNA construct encoding a scrambled sequence that will not lead to the million base pairs and comprises nearly 6% of the human genome. Deletion specific degradation of any known cellular mRNA. of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. GENE EXPRESSION MONITORING Additionally, Porphyria cutanea tarda, Parkinson’s disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map BAT2 (A-10): sc-373747 is recommended as a control antibody for monitoring to chromosome 6. of BAT2 gene expression knockdown by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) or immunofluorescence (starting dilution REFERENCES 1:50, dilution range 1:50-1:500). 1. Banerji, J., et al. 1990. A gene pair from the human major histocompatibility To ensure optimal results, the following support reagents are recommended: complex encodes large proline-rich proteins with multiple repeated motifs 1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (Cruz and a single ubiquitin-like domain. Proc. Natl. Acad. Sci. USA 87: 2374-2378. Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ Molecular Weight Standards: sc-2035, UltraCruz® Blocking Reagent: 2. Hashimoto, M., et al. 1999. Genetic contribution of the BAT2 gene sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- microsatellite polymorphism to the age-at-onset of Insulin-dependent rescence: use m-IgGκ BP-FITC: sc-516140 or m-IgGκ BP-PE: sc-516141 diabetes mellitus. Hum. Genet. 105: 197-199. (dilution range: 1:50-1:200) with UltraCruz® Mounting Medium: sc-24941 3. Online Mendelian Inheritance in Man, OMIM™. 2001. Johns Hopkins or UltraCruz® Hard-set Mounting Medium: sc-359850. University, Baltimore, MD. MIM Number: 142580. World Wide Web URL: http://www.ncbi.nlm.nih.gov/omim/ RT-PCR REAGENTS 4. Martinez, A., et al. 2004. Association of the major histocompatibility com- Semi-quantitative RT-PCR may be performed to monitor BAT2 gene expres- plex with response to infliximab therapy in rheumatoid arthritis patients. sion knockdown using RT-PCR Primer: BAT2 (h)-PR: sc-72612-PR (20 µl). Arthritis Rheum. 50: 1077-1082. Annealing temperature for the primers should be 55-60° C and the extension temperature should be 68-72° C. 5. Schneiders, A., et al. 2005. Antibodies generated by a novel DNA vaccina- tion identify the MHC class III encoded BAT2 polypeptide. Vaccine 23: BIOSAFETY 2540-2550. Lentiviral particles can be employed in standard Biosafety Level 2 tissue CHROMOSOMAL LOCATION culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). Lentiviral particles are repli- Genetic locus: BAT2 (human) mapping to 6p21.33. cation-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. PRODUCT BAT2 shRNA (h) Lentiviral Particles is a pool of concentrated, transduction- RESEARCH USE ready viral particles containing 3 target-specific constructs that encode The purchase of this product conveys to the buyer the nontransferable right 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each to use the purchased amount of the product and all replicates and derivatives 6 vial contains 200 µl frozen stock containing 1.0 x 10 infectious units of virus for research purposes conducted by the buyer in his laboratory only (whether (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suit- the buyer is an academic or for-profit entity). The buyer cannot sell or other- able for 10-20 transductions. Also see BAT2 siRNA (h): sc-72612 and BAT2 wise transfer (a) this product (b) its components or (c) materials made using shRNA Plasmid (h): sc-72612-SH as alternate gene silencing products. this product or its components to a third party, or otherwise use this product or its components or materials made using this product or its components STORAGE for Commercial Purposes. Store lentiviral particles at -80º C. Stable for at least one year from the date of shipment. Once thawed, particles can be stored at 4º C for up to PROTOCOLS one week. Avoid repeated freeze thaw cycles. See our web site at www.scbt.com for detailed protocols and support products. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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