Defining the elastic fiber interactome

Supplemental Information

Defining elastic fiber interactions by molecular fishing: an affinity purification and mass spectrometry approach

Stuart A. Cain*, Amanda McGovern, Elaine Small, Lyle J. Ward, Clair Baldock, Adrian Shuttleworth and Cay M. Kielty Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, United Kingdom. *Corresponding author

Supplemental Fig. 1

CPL - + - + - + DTT

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Supplemental Fig.1 4-12% bis-Tris gel showing purified calsyntenin-1 (C), proepithelin (P) and LOX (L), with (+) and without (-) reduction with DTT.

1 Defining the elastic fiber interactome SUPPLEMENTAL TABLE 1A Peptides detected no-bait controls No-bait protein (bead only) controls were carried out for all four cell compartments Samples were processed as described in the Experimental Procedures, with the exception that no bait protein was added. Scaffold was used to validate protein and peptide identification. Only with >2 unique peptide sequences were counted, and each peptide had greater than 95.0% probability as specified by the Peptide Prophet algorithm (38). Shown is the number of peptides detected,with the number of unique peptide sequence number in brackets. Also shown is the experiment number for which the peptides were detected. Experiments were numbered in the sequence they were conducted and are labelled with the prefix 'ex'.

SwissProt Entry ARPE Matrix ARPE Media HDF Matrix HDF Media Protein Protein Identitiy Name ex11 ex169 ex173 ex167 ex171 ex12 ex157 ex158 ex165 ex155 ex163 ex8 Total Fibronectin FINC_HUMAN 2 (2) 2 (2) Histone H3.3 H33_HUMAN 3 (2) 3 (2) Histone H4 H4_HUMAN 12 (7) 12 (7) , type I cytoskeletal 9 K1C9_HUMAN 9 (8) 9 (8) Keratin, type II cytoskeletal 1 K2C1_HUMAN 7 (7) 7 (7) Lymphoid-restricted LRMP_HUMAN 2 (2) 2 (2) Protocadherin-19 PCD19_HUMAN 2(2) 2 (2) VIME_HUMAN 14 (13) 14 (13) Grand Total 33 (26) 000002 (2)0016 (15) 0 0 51 (43)

SUPPLEMENTAL TABLE 1B Peptides detected cell culture compartment controls Controls were carried out for all four cell compartments Samples were processed as described in the Experimental Procedures, with the exception that no bait protein was added and the samples did not undergo affinity capture LC . Scaffold was used to validate protein and peptide identification. Only proteins with >2 unique peptide sequences were counted, and each peptide had greater than 95.0% probability as specified by the Peptide Prophet algorithm (38). Shown is the number of peptides detected (indicated Pep. No), with the number of unique peptide sequence number in brackets . Also shown is the % coverage for each protein.

Protein Identity SwissProt Entry ARPE Matrix ARPE Media HDF Matrix HDF Media Name Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Serum albumin ALBU_BOVIN 16 (6) 8.6 118 (20) 29 45 (10) 17 97 (14) 21 , cytoplasmic ACTB_HUMAN 63 (12) 36 15 (5) 18 Vimentin VIME_HUMAN 13 (9) 22 43 (20) 44 Alpha-2-HS-glycoprotein FETUA_BOVIN 2 (2) 4.7 7 (4) 18 10 (4) 11 Serum albumin ALBU_HUMAN 11 (2) 3.1 Keratin, type II cytoskeletal 1 K2C1_HUMAN 6 (4) 12 14 (7) 14 Alpha-1-antiproteinase A1AT_BOVIN 4 (3) 8.4 Keratin, type I cytoskeletal 18 K1C18_HUMAN 13 (8) 18 Keratin, type I cytoskeletal 10 K1C10_HUMAN 6 (3) 6.7 6 (3) 4.7 Histone H4 H4_HUMAN 9 (5) 48 Keratin, type II cytoskeletal 2 epidermal K22E_HUMAN 3 (3) 3.6 Serotransferrin TRFE_BOVIN 5 (4) 4.7 Keratin, type II cytoskeletal 8 K2C8_HUMAN 4 (3) 6.4 Keratin, type II cytoskeletal 7 K2C7_HUMAN 4 (4) 5.1 Heterogeneous nuclear ribonucleoproteins A2/B1 ROA2_BOVIN 3 (2) 12

Supplemental Table 1 2 Defining the elastic fiber interactome SUPPLEMENTAL TABLE 2 Total number of peptides and unique peptide sequences for each protein detected after Scaffold validation Scaffold was used to validate protein and peptide identification. Only proteins with >2 unique peptide sequences were counted and each peptide had greater than 95.0% probability as specified by the Peptide Prophet algorithm (38). The proteins were then ranked by total number of peptides detected. The cell culture compartments in which the identified peptides were detected are also given. Also shown is the number of unique peptide sequence in brackets, and the total % coverage of the unique peptide sequences (calculated as described in Experimental Procedures). Peptides detected from bait proteins were included (shown in bold). The global % ubiquity was calculated for each detected protein using BEPro3. Proteins with a % ubiquity greater than 40% were treated as non-specific contaminants (indicated *). The sequences of the peptides identified for each protein are shown in Supplemental Table 4.

Protein Identity SwissProt Entry ARPE Matrix ARPE Media HDF Matrix HDF Media Protein Peptide Total % Ubiquity Name Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov -1 FBN1_HUMAN 394 (76) 35.6 760 (115) 55.8 334 (58) 26.6 649 (118) 56.5 2137 (145) 65.8 51.4 Keratin type II cytoskeletal 1 * K2C1_HUMAN 167 (34) 42.9 130 (25) 38.7 154 (32) 41.1 181 (33) 43.6 632 (42) 49.7 86.9 inhibitor 1 PAI1_HUMAN 33 (15) 49.3 320 (21) 65.4 2 (2) 6.0 54 (18) 60.4 409 (22) 65.4 38.0 PGBM_HUMAN 117 (71) 22.1 256 (96) 31.5 23 (20) 5.5 2 (2) 0.7 398 (113) 36.5 18.6 Keratin type I cytoskeletal 10 * K1C10_HUMAN 96 (32) 54.5 37 (20) 45.7 63 (21) 38.3 123 (31) 52.8 319 (40) 63.9 48.0 Keratin type I cytoskeletal 9 * K1C9_HUMAN 46 (16) 32.3 45 (15) 39.2 63 (14) 30.0 49 (21) 37.9 203 (25) 47.4 50.2 BigH3 BGH3_HUMAN 7 (6) 9.4 123 (23) 47.0 2 (2) 6.0 132 (23) 47.0 7.3 Histone H4. H4_HUMAN 66 (7) 52.4 18 (5) 50.5 32 (7) 52.4 12 (4) 42.7 128 (7) 52.4 14.6 Fibronectin FINC_HUMAN 2 (2) 0.7 57 (37) 23.6 26 (24) 14.8 19 (16) 10.2 104 (49) 30.6 17.8 Stanniocalcin-2 STC2_HUMAN 8 (6) 27.5 44 (6) 27.5 6 (6) 27.5 45 (6) 24.8 103 (7) 30.1 38.2 Thrombospondin-1 TSP1_HUMAN 93 (28) 33.2 5 (4) 6.0 98 (28) 33.2 2.0 Proepithelin GRN_HUMAN 21 (11) 21.8 46 (15) 32.2 5 (4) 9.6 17 (9) 16.5 89 (19) 34.2 8.2 Keratin type II * K22E_HUMAN 15 (18) 20.0 6 (9) 15.8 25 (22) 30.1 41 (27) 35.0 87 (36) 45.0 48.6 Sulfhydryl oxidase 1 QSOX1_HUMAN 67 (28) 47.8 5 (4) 8.3 72 (29) 50.3 0.4 Serum albumin ALBU_HUMAN 10 (7) 11.2 15 (5) 8.7 18 (8) 12.0 22 (8) 13.1 65 (12) 18.2 22.2 Collagen alpha-1(XVIII) chain COIA1_HUMAN 26 (10) 7.4 36 (13) 11.3 62 (15) 13.0 3.4 -A/C LMNA_HUMAN 17 (8) 14.6 42 (17) 31.6 59 (19) 34.5 8.1 Calsyntenin-1 CSTN1_HUMAN 29 (16) 19.9 16 (14) 18.1 45 (19) 24.1 2.9 Agrin AGRIN_HUMAN 42 (21) 12.8 42 (21) 12.8 6.4 Collagen alpha-1(I) chain CO1A1_HUMAN 5 (4) 4.6 36 (21) 24.1 41 (22) 25.3 11.4 Insulin-like growth factor-binding protein 7 IBP7_HUMAN 2 (2) 10.6 38 (11) 51.8 40 (11) 51.8 0.4 Keratin type I cytoskeletal 14 K1C14_HUMAN 2 (10) 10.0 2 (3) 7.4 15 (16) 26.1 18 (14) 24.2 37 (25) 38.1 8.7 Collagen alpha-1(IV) chain CO4A1_HUMAN 30 (8) 7.5 5 (4) 3.5 35 (8) 7.5 0.3 Insulin-like growth factor-binding protein 4 IBP4_HUMAN 2 (2) 11.2 30 (7) 31.0 32 (7) 31.0 0.4 MAGP-1 MFAP2_HUMAN 2 (2) 9.8 13 (5) 25.1 17 (6) 35.5 32 (6) 35.5 4.1 Collagen alpha-2(IV) chain CO4A2_HUMAN 31 (13) 16.1 0.0 31 (13) 16.1 0.4 Calsyntenin-2 CSTN2_HUMAN 30 (16) 21.4 30 (16) 21.4 2.3 Collagen alpha-2(I) chain CO1A2_HUMAN 28 (18) 23.4 28 (18) 23.4 3.3 Annexin A2 ANXA2_HUMAN 2 (2) 5.6 24 (15) 50.7 26 (15) 50.7 4.2 Keratin type II cytoskeletal 6 K2C6A_HUMAN 5 (4) 14.4 15 (12) 31.4 6 (4) 19.5 26 (20) 43.1 7.5 Insulin-like growth factor-binding protein 3 IBP3_HUMAN 21 (7) 24.4 4 (3) 13.1 25 (7) 24.4 5.4 TGF beta-2 TGFB2_HUMAN 3 (3) 8.5 22 (10) 30.9 25 (10) 30.9 0.6 Histone H2B H2B1M_HUMAN 18 (5) 52.4 4 (3) 27.8 2 (2) 15.9 24 (6) 52.4 2.4 Keratin type II cytoskeletal 5 K2C5_HUMAN 3 (3) 12.5 3 (3) 14.6 16 (14) 26.1 22 (14) 33.2 7.4 Retinoic acid receptor responder protein 2 RARR2_HUMAN 22 (5) 39.3 22 (5) 39.3 1.7 -9 MYH9_HUMAN 16 (12) 8.8 2 (2) 1.4 3 (3) 1.9 21 (13) 9.5 0.4 Protein NOV homolog NOV_HUMAN 21 (9) 36.1 21 (9) 36.1 0.4 Extracellular matrix protein 1 ECM1_HUMAN 20 (11) 29.1 20 (11) 29.1 0.3 Histone H2A type 2-A H2A2A_HUMAN 11 (4) 59.2 6 (4) 35.4 2 (2) 12.3 19 (5) 67.7 0.4 Collagen alpha-1(XII) chain COCA1_HUMAN 18 (15) 6.4 18 (15) 6.4 0.4 TITIN_HUMAN 3 (3) 0.2 11 (11) 0.7 2 (2) 0.1 16 (16) 0.9 0.4 Keratin type I cytoskeletal K1C16_HUMAN 15 (10) 33.8 15 (10) 33.8 7.5 Supplemental Table 2 3 Defining the elastic fiber interactome Protein Identity SwissProt Entry ARPE Matrix ARPE Media HDF Matrix HDF Media Protein Peptide Total % Ubiquity Name Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Protein S100-A7 S10A7_HUMAN 4 (3) 33.7 10 (5) 54.5 14 (5) 54.5 2.1 Vimentin VIME_HUMAN 2 (2) 3.6 11 (11) 27.9 13 (13) 31.5 4.9 Annexin V ANXA5_HUMAN 2 (2) 6.6 4 (4) 15.0 3 (3) 11.9 9 (6) 21.9 4.1 Actin cytoplasmic 1 ACTB_HUMAN 5 (3) 7.5 3 (3) 10.4 8 (5) 14.9 1.6 Follistatin-related protein FSTL1_HUMAN 8 (6) 31.5 8 (6) 31.5 0.3 Lysyl oxidase LYOX_HUMAN 3 (3) 8.6 5 (4) 12.7 8 (5) 15.3 4.1 Zinc-alpha-2-glycoprotein ZA2G_HUMAN 9(9) 1.5 8 (9) 1.5 0.3 Fibulin-1 FBLN1_HUMAN 7 (5) 8.1 7 (5) 8.1 3.6 Fibrillin-3 FBN3_HUMAN 6 (2) 0.8 6 (2) 1.6 0.3 Histone H3 H33_HUMAN 2 (2) 11.0 2 (2) 11.0 2 (2) 11.8 6 (3) 16.2 0.4 Insulin-like growth factor-binding protein 5 IBP5_HUMAN 6 (4) 18.0 6 (4) 18.0 0.4 LTBP-2 LTBP2_HUMAN 6 (5) 3.5 6 (5) 3.5 3.1 Single-stranded DNA-binding protein ALBU_HUMAN 11.2 6 (5) 8.7 12.0 13.1 6 (5) 18.2 0.3 beta chain brain 1 SPTB2_HUMAN 6 (3) 1.7 6 (3) 1.7 0.3 Protein CYR61 CYR61_HUMAN 5 (4) 12.1 5 (4) 12.1 0.3 Protein S100-A9 S10A9_HUMAN 3 (3) 31.6 2 (2) 26.3 5 (4) 44.7 1.8 Tenascin TENA_HUMAN 5 (4) 3.1 5 (4) 3.1 0.4 Alpha-2-macroglobulin A2MG_HUMAN 2 (2) 2.4 2 (2) 2.4 4 (2) 2.4 3.2 Dedicator of cytokinesis protein 2 DOCK2_HUMAN 4 (2) 2.4 4 (2) 2.4 0.4 Plexin domain-containing protein 2 PXDC2_HUMAN 4 (3) 8.9 4 (3) 8.9 0.3 Vacuolar protein sorting-associated protein VP13D_HUMAN 2 (2) 1.5 4 (4) 1.5 0.4 Zinc transporter ZIP10 S39AA_HUMAN 4 (2) 2.9 4 (2) 2.9 0.4 Amyloid A4_HUMAN 3 (3) 6.2 3 (3) 6.2 0.3 Apolipoprotein D APOD_HUMAN 3 (3) 16.4 3 (3) 16.4 0.3 ATP-binding cassette sub-family A member 13 ABCAD_HUMAN 3 (3) 1.3 3 (3) 1.3 0.4 Bullous pemphigoid antigen 1 isoforms BPA1_HUMAN 1/2/3/4/5/8 3 (3) 1.5 3 (3) 1.5 0.4 Chromodomain-helicase-DNA-binding protein 5 CHD5_HUMAN 3 (2) 1.5 3 (2) 1.5 0.4 Collagen alpha-1(XI) chain COBA1_HUMAN 3 (3) 2.9 3 (3) 2.9 0.3 Heterogeneous nuclear ribonucleoprotein M HNRPM_HUMAN 3 (2) 5.9 3 (2) 5.9 0.4 Matrilin-2 MATN2_HUMAN 3 (3) 4.0 3 (3) 4.0 0.3 Prolactin-inducible protein PIP_HUMAN 3 (3) 26.0 3 (3) 26.0 0.3 Protocadherin-17 PCD17_HUMAN 3 (2) 3.4 3 (2) 3.4 0.3 Vacuolar protein sorting-associated protein 13A VP13A_HUMAN 3 (2) 1.5 3 (2) 1.5 0.4 Activated RNA polymerase II TCP4_HUMAN 2 (2) 19.7 2 (2) 19.7 0.3 A- anchor protein 9 AKAP9_HUMAN 2 (2) 0.9 2 (2) 0.9 0.4 Amine oxidase AOFA_HUMAN 2 (2) 7.4 2 (2) 7.4 0.3 Bile salt export pump ABCBB_HUMAN 2 (2) 3.5 2 (2) 3.5 0.4 Bullous pemphigoid antigen 1 isoforms 6/9/10 BPAEA_HUMAN 2 (2) 0.9 2 (2) 0.9 0.4 Cadherin-related tumor suppressor homolog FAT1_HUMAN 2 (2) 1.1 2 (2) 1.1 0.4 Cathepsin H CATH_HUMAN 2 (2) 8.4 2 (2) 8.4 0.3 Coiled-coil domain-containing protein CCD80_HUMAN 2 (2) 2.6 2 (2) 2.6 0.4 Collagen alpha-1(VIII) chain CO8A1_HUMAN 2 (2) 3.9 2 (2) 3.9 3.2 Collagen alpha-3(IV) chain CO4A3_HUMAN 2 (3) 3.8 2 (3) 3.8 0.3 Collagen alpha-3(V) chain CO5A3_HUMAN 2 (2) 3.4 2 (2) 3.4 0.4 Complement C1q C1QT3_HUMAN 2 (2) 12.2 2 (2) 12.2 6.9 Complement C3 CO3_HUMAN 2 (2) 1.4 2 (2) 1.4 0.3 Complement factor D CFAD_HUMAN 2 (2) 19.0 2 (2) 19.0 0.3

Supplemental Table 2 4 Defining the elastic fiber interactome Protein Identity SwissProt Entry ARPE Matrix ARPE Media HDF Matrix HDF Media Protein Peptide Total % Ubiquity Name Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Pep. No. % Cov Dickkopf-related protein 1 DKK1_HUMAN 2 (2) 7.9 2 (2) 7.9 0.3 Elastin ELN_HUMAN 2 (2) 4.2 2 (2) 4.2 2.1 Fibrillin-2 FBN2_HUMAN 2 (2) 0.7 2 (2) 0.7 17.6 Fibulin-3 FBLN3_HUMAN 2 (2) 6.5 2 (2) 6.5 0.3 Golgin subfamily B member 1 GOGB1_HUMAN 2 (2) 1.3 2 (2) 1.3 0.4 Hemoglobin subunit alpha HBA_HUMAN 2 (2) 21.8 2 (2) 21.8 0.3 High mobility group protein HMGA1_HUMAN 2 (2) 23.4 2 (2) 23.4 0.3 Insulin-like growth factor II IGF2_HUMAN 2 (2) 13.9 2 (2) 13.9 0.3 Keratin type II cuticular Hb1 KRT81_HUMAN 2 (2) 3.4 2 (2) 3.4 0.3 LOXL LOXL1_HUMAN 2 (2) 5.9 2 (2) 5.9 0.4 Neutrophil defensin 13 DEF1_HUMAN 2 (2) 19.1 2 (2) 19.1 0.4 OBSCN_HUMAN 2 (2) 0.6 2 (2) 0.6 0.4 Probable JmjC domain-containing histone demethylation protein 2C JHD2C_HUMAN 2 (2) 1.5 2 (2) 1.5 0.4 Protein disulfide A6 PDIA6_HUMAN 2 (2) 7.3 2 (2) 7.3 0.3 Protein mobA MOBA_THIFE 2 (2) 13.0 2 (2) 13.0 0.4 Protein S100-A8 S10A8_HUMAN 2 (2) 23.7 2 (2) 23.7 0.7 RUN domain-containing protein 2A RUN2A_HUMAN 2 (2) 12.5 2 (2) 12.5 0.4 Ryanodine receptor 3 RYR3_HUMAN 2 (2) 0.8 2 (2) 0.8 0.4 Serine/-protein kinase SMG1 SMG1_HUMAN 2 (2) 1.1 2 (2) 1.1 0.4 Striated muscle preferentially expressed protein kinase SPEG_HUMAN 2 (2) 1.0 2 (2) 1.0 0.4

Trinucleotide repeat-containing 6A protein TNR6A_HUMAN 2 (2) 2.3 2 (2) 2.3 0.3 Tyrosine-protein kinase BTK BTK_HUMAN 2 (2) 3.9 2 (2) 3.9 0.4 Uncharacterized protein C6orf174 CF174_HUMAN 2 (2) 4.3 2 (2) 4.3 0.4 Grand Total 1200 (470) 2432 (683) 925 (374) 1475 (500) 6032 (1211)

Supplemental Table 2 5 Defining the elastic fiber interactome SUPPLEMENTAL TABLE 3 Complete list of prey proteins identified for each bait protein Table showing a complete list of each prey protein identified using each recombinant bait protein (bold). Shown is the SwissProt entry name, the averaged Bayes’ odds for each bait-prey pair, the false positive rate, and the % reproducibility generated from the Scaffold and Phenyx data using BEPro3 software. The total number of peptides identified with Scaffold and the total number of peptides identified using Phenyx (with the number of unique peptide sequences shown in brackets), and the total Phenyx score. All proteins listed from both search engines were identified by a minimum of 2 unique peptides sequences. The four highly ubiquitous identified in Table 1 have been removed. The sequence of each peptide identified is shown in Supplemental Table 5.

Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score Calsyntenin-1 Ex Calsyntenin-1 CSTN1_HUMAN 0.1715 0.037 24 (14) 22 (15) 180.6 44 Perlecan PGBM_HUMAN 0.1715 0.191 22 (12) 16 (9) 130.5 44 Fibrillin-1 FBN1_HUMAN 0.1715 0.162 5 (3) 5 (3) 42.5 44 Histone H4. H4_HUMAN 0.111 0.180 20 (6) 26 (7) 187.1 33 Plasminogen activator inhibitor 1 PAI1_HUMAN 0 0.325 8 (8) 6 (5) 45.1 22 Histone H2B H2B1M_HUMAN 0 0.057 6 (3) 23 (3) 175.0 22 Fibronectin FINC_HUMAN 0 0.190 6 (6) 6 (6) 22.0 22 Annexin A2 ANXA2_HUMAN 0 0.051 5 (5) 4 (4) 34.1 22 Lamin-A/C LMNA_HUMAN 0 0.101 3 (3) 4 (4) 29.1 22 Insulin-like growth factor-binding protein 7 IBP7_HUMAN 0 0.048 3 (3) 2 (2) 7.1 22 Tenascin TENA_HUMAN 0 0.027 2 (2) 3 (3) 16.2 22 Agrin AGRIN_HUMAN 0 0.074 2 (2) 2 (2) 8.5 22 Serum albumin ALBU_HUMAN 0 0.102 2 (2) 20 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 2 (2) 20 Titin TITIN_HUMAN 0 0.037 2 (2) 20

Collagen alpha-1(VIII) NC2 Collagen alpha-1(VIII) chain CO8A1_HUMAN 0.4905 0.024 4 (2) 5 (1) 36.2 32 Fibronectin FINC_HUMAN 0.19 0.190 16 (13) 10 (9) 69.5 42 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.112 14 (7) 4 (2) 28.5 32 Lamin-A/C LMNA_HUMAN 0 0.101 10 (4) 21 (4) 164.5 21 Keratin type II cytoskeletal 6 K2C6A_HUMAN 0 0.112 4 (4) 6 (2) 51.9 21 Hornerin HORN_HUMAN 0 0.009 3 (3) 3 (3) 25.5 11 Fibrillin-1 FBN1_HUMAN 0 0.162 3 (3) 2 (2) 14.5 16 Keratin type I cytoskeletal 14 K1C14_HUMAN 0 0.126 2 (2) 4 (3) 19.3 11 Keratin type II cytoskeletal 5 K2C5_HUMAN 0 0.095 2 (3) 4 (3) 24.5 16 Annexin A2 ANXA2_HUMAN 0 0.051 2 (2) 3 (2) 29.3 16 Collagen alpha-2(I) chain CO1A2_HUMAN 0 0.061 2 (2) 3 (1) 25.2 16 Plasminogen activator inhibitor 1 PAI1_HUMAN 0 0.325 2 (2) 3 (3) 26.0 16 Titin TITIN_HUMAN 0 0.037 2 (2) 11 Histone H4. H4_HUMAN 0 0.207 6 (2)50.8 20 Growth differentiation factor 15 GDF15_HUMAN 0 0.043 2 (2)18.1 20

Collagen alpha-1(VIII) Full Length Collagen alpha-2(VIII) chain CO8A2_HUMAN 1 0.002 9 (3) 5 (2) 39.0 64 Fibronectin FINC_HUMAN 0.989 0.190 6 (5) 13 (11) 75.6 64 Vimentin VIME_HUMAN 0.358 0.034 26 (13) 19 (11) 123.4 45 Lamin-A/C LMNA_HUMAN 0.012 0.101 30 (19) 19 (17) 104.8 36 Collagen alpha-1(I) chain CO1A1_HUMAN 0.012 0.112 10 (7) 4 (4) 36.4 36 Histone H4. H4_HUMAN 0.0035 0.180 12 (4) 8 (3) 65.1 36 Histone H2B H2B1M_HUMAN 0 0.057 2 (2) 18 (2) 141.7 18 Stanniocalcin-2 STC2_HUMAN 0 0.307 2 (2) 2 (2) 8.5 18 Tenascin-X TENX_HUMAN 0 0.002 2 (2) 20 Supplemental Table 3 6 Defining the elastic fiber interactome Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score THAP domain-containing protein 4 THAP4_HUMAN 0 0.002 2 (2) 20 DESM_HUMAN 0 0.009 2 (2) 7.0 17 Plasminogen activator inhibitor 1 PAI1_HUMAN 0 0.346 2 (2)16.2 33

Collagen alpha-2(VIII) NC2 Lamin-A/C LMNA_HUMAN 0.16 0.101 2 (2) 5 (3) 41.0 38 Serum albumin ALBU_HUMAN 0.0725 0.185 2 (2) 3 (2) 23.2 38 N-acetylmuramic acid 6-phosphate etherase MURQ_SHIDS 0.001 0.002 2 (2) 25 Fibronectin FINC_HUMAN 0 0.190 6 (6) 2 (2) 14.5 25 Annexin A2 ANXA2_HUMAN 0 0.051 3 (3) 4 (4) 17.3 25 Perlecan PGBM_HUMAN 0 0.191 3 (3) 2 (2) 8.5 25

Fibulin 5 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.4015 0.325 18 (11) 19 (10) 145.8 42 Keratin type II cytoskeletal 5 K2C5_HUMAN 0.0135 0.095 9 (8) 16 (5) 116.6 37 Keratin type II cytoskeletal 6 K2C6A_HUMAN 0.0135 0.112 3 (5) 11 (2) 54.4 26 Fibrillin-1 FBN1_HUMAN 0 0.162 28 (23) 28 (22) 208.7 26 Keratin type I cytoskeletal 14 K1C14_HUMAN 0 0.126 12 (10) 14 (6) 102.8 32 Histone H4. H4_HUMAN 0 0.180 12 (5) 12 (4) 79.6 26 Perlecan PGBM_HUMAN 0 0.191 8 (8) 10 (10) 50.0 11 TGF beta-2 TGFB2_HUMAN 0 0.033 6 (6) 5 (5) 14.0 11 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 6 (6) 11 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0 0.081 5 (5) 9 (5) 67.8 11 Stanniocalcin-2 STC2_HUMAN 0 0.307 4 (3) 8 (4) 51.2 26 Agrin AGRIN_HUMAN 0 0.074 4 (4) 4 (4) 29.1 11 Serum albumin ALBU_HUMAN 0 0.185 4 (4) 4 (4) 24.5 21 Insulin-like growth factor-binding protein 7 IBP7_HUMAN 0 0.048 4 (4) 3 (3) 16.9 11 Histone H2A type 2-A H2A2A_HUMAN 0 0.017 4 (2) 2 (2) 7.0 11 Myosin-9 MYH9_HUMAN 0 0.024 3 (3) 4 (4) 32.8 11 Collagen alpha-2(I) chain CO1A2_HUMAN 0 0.061 3 (3) 2 (2) 8.4 11 Fibronectin FINC_HUMAN 0 0.190 3 (3) 2 (2) 14.4 11 Granulins (Proepithelin) GRN_HUMAN 0 0.044 3 (3) 2 (2) 18.7 11 Retinoic acid receptor responder protein 2 RARR2_HUMAN 0 0.052 2 (2) 4 (2) 38.3 11 Keratin type II cuticular Hb1 KRT81_HUMAN,KRT 0 0.009 2 (2) 3 (1) 23.9 11 Protein S100-A8 S10A8_HUMAN 0 0.026 2 (2) 2 (2) 16.5 11 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.112 2 (2) 1 (1) 7.3 11 Histone H2B H2B1M_HUMAN 0 0.077 2 (2) 11 Histone H2A H2A1A_HUMAN 0 0.009 28 (2)98.5 10 Collagen alpha-1(VIII) chain CO8A1_HUMAN 0 0.038 3 (3)15.2 10 LTBP-2 LTBP2_HUMAN 0 0.044 3 (3)14.9 20 Lysozyme C LYSC_HUMAN 0 0.009 2 (2)14.4 10 Protein S100-A9 S10A9_HUMAN 0 0.058 2 (2)15.7 10

Lysyl Oxidase Lysyl oxidase LYOX_HUMAN 0.975 0.009 8 (5) 10 (6) 94.0 54 Stanniocalcin-2 STC2_HUMAN 0.551 0.307 8 (2) 7 (2) 62.8 54 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.511 0.325 10 (6) 8 (4) 64.1 54 Fibrillin-1 FBN1_HUMAN 0.3975 0.162 35 (21) 44 (28) 323.2 46 Perlecan PGBM_HUMAN 0.3975 0.191 25 (18) 24 (19) 186.1 46 Collagen alpha-1(XII) chain COCA1_HUMAN 0 0.009 9 (9) 9 (9) 67.9 15 Thrombospondin-1 TSP1_HUMAN 0 0.074 8 (5) 8 (5) 38.5 15 Insulin-like growth factor-binding protein 4 IBP4_HUMAN 0 0.045 6 (4) 5 (4) 34.7 15 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0 0.081 3 (3) 4 (4) 15.9 15 Protein NOV homolog NOV_HUMAN 0 0.051 3 (3) 2 (2) 10.3 15

Supplemental Table 3 7 Defining the elastic fiber interactome Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score Tenascin TENA_HUMAN 0 0.027 3 (3) 2 (2) 18.2 15 Insulin-like growth factor-binding protein 5 IBP5_HUMAN 0 0.034 2 (2) 5 (3) 29.9 15 Fibronectin FINC_HUMAN 0 0.190 2 (2) 3 (3) 24.9 15 Fibulin-1 FBLN1_HUMAN 0 0.019 2 (2) 3 (3) 26.4 15 Serine/threonine-protein kinase SMG1 SMG1_HUMAN 0 0.009 2 (2) 14 Insulin-like growth factor-binding protein 3 IBP3_HUMAN 0 0.047 3 (2)14.7 17

MAGP-1 MAGP-1 MFAP2_HUMAN 1 0.002 32 (6) 18 (5) 132.5 79 Serum albumin ALBU_HUMAN 1 0.185 30 (6) 25 (8) 156.5 100 Perlecan PGBM_HUMAN 0.995 0.191 167 (77) 117 (60) 870.3 57 Fibrillin-1 FBN1_HUMAN 0.995 0.162 35 (18) 19 (12) 145.5 57 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.9825 0.325 71 (17) 34 (16) 211.7 57 Alpha-2-macroglobulin A2MG_HUMAN 0.4985 0.009 4 (2) 6 (1) 55.7 43 Fibronectin FINC_HUMAN 0.102 0.190 28 (18) 24 (15) 158.7 43 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0.102 0.081 15 (8) 9 (5) 74.7 43 Stanniocalcin-2 STC2_HUMAN 0.102 0.307 12 (5) 10 (6) 80.0 43 Histone H4. H4_HUMAN 0.02 0.180 10 (4) 10 (3) 89.3 29 Polypyrimidine tract-binding protein 1 PTBP1_HUMAN 0.002 0.002 4 (2)30.2 29 BigH3 BGH3_HUMAN 0 0.093 46 (15) 32 (14) 250.0 29 Calsyntenin-2 CSTN2_HUMAN 0 0.023 16 (10) 11 (8) 60.9 29 Lamin-A/C LMNA_HUMAN 0 0.101 14 (8) 8 (7) 58.8 29 Retinoic acid receptor responder protein 2 RARR2_HUMAN 0 0.052 11 (4) 16 (4) 134.1 29 Thrombospondin-1 TSP1_HUMAN 0 0.074 11 (7) 11 (7) 74.6 29 Calsyntenin-1 CSTN1_HUMAN 0 0.037 11 (7) 10 (7) 55.1 29 Insulin-like growth factor-binding protein 7 IBP7_HUMAN 0 0.048 10 (7) 9 (8) 58.8 29 Insulin-like growth factor-binding protein 4 IBP4_HUMAN 0 0.045 9 (4) 4 (3) 31.8 14 Myosin-9 MYH9_HUMAN 0 0.024 7 (5) 6 (4) 46.4 29 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 7 (5) 29 Spectrin beta chain brain 1 SPTB2_HUMAN 0 0.009 6 (3) 4 (2) 34.7 14 Collagen alpha-1(IV) chain CO4A1_HUMAN 0 0.023 6 (3) 3 (3) 26.8 14 Histone H2A type 2-A H2A2A_HUMAN,H2A 0 0.025 6 (4) 29 Actin cytoplasmic 1 ACTB_HUMAN,ACT 0 0.048 5 (4) 6 (2) 41.0 29 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.112 5 (5) 1 (1) 9.2 14 Insulin-like growth factor-binding protein 3 IBP3_HUMAN 0 0.052 4 (2) 4 (3) 22.9 29 Zinc transporter ZIP10 S39AA_HUMAN 0 0.009 4 (2) 14 Protein CYR61 CYR61_HUMAN 0 0.019 3 (3) 3 (3) 14.9 14 Titin TITIN_HUMAN 0 0.037 3 (3) 14 Insulin-like growth factor-binding protein 5 IBP5_HUMAN 0 0.034 2 (2) 4 (4) 23.1 14 Protein NOV homolog NOV_HUMAN 0 0.051 2 (2) 4 (4) 21.5 14 Insulin-like growth factor II IGF2_HUMAN 0 0.009 2 (2) 3 (3) 7.7 14 LTBP-2 LTBP2_HUMAN 0 0.030 2 (2) 3 (3) 9.2 14 Dickkopf-related protein 1 DKK1_HUMAN 0 0.013 2 (2) 2 (2) 16.2 14 Collagen alpha-3(IV) chain CO4A3_HUMAN 0 0.009 2 (2) 1 (1) 7.4 14 Collagen alpha-2(IV) chain CO4A2_HUMAN 0 0.023 2 (2) 14 Collagen alpha-3(V) chain CO5A3_HUMAN 0 0.009 2 (2) 14 Golgin subfamily B member 1 GOGB1_HUMAN 0 0.009 2 (2) 14 Histone H2B H2B1M_HUMAN 0 0.077 2 (2) 14 Protein mobA MOBA_THIFE 0 0.009 2 (2) 14 RUN domain-containing protein 2A RUN2A_HUMAN 0 0.009 2 (2) 14 Striated muscle preferentially expressed protein kinase SPEG_HUMAN 0 0.009 2 (2) 14 Vacuolar protein sorting-associated protein VP13D_HUMAN 0 0.015 2 (2) 14

Supplemental Table 3 8 Defining the elastic fiber interactome Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score Actin cytoplasmic 1 (Beta-actin) ACTB_HUMAN 0 0.058 6 (2)41.0 29 Actin aortic smooth muscle (Alpha-actin-2) ACTA_HUMAN 0 0.058 5 (4)33.0 29 Apolipoprotein D APOD_HUMAN 0 0.015 4 (4)34.1 14 Prolactin-inducible protein PIP_HUMAN 0 0.016 4 (3)33.1 29 Collagen alpha-1(V) chain CO5A1_HUMAN 0 0.023 3 (2)22.4 29 Neutrophil defensin 1 DEF1_HUMAN 0 0.009 2 (2) 8.1 14 Protein S100-A8 S10A8_HUMAN 0 0.043 2 (2)17.7 29 Small breast epithelial mucin MUCL_HUMAN 0 0.015 2 (2) 7.2 14

PF1 Fibrillin-1 FBN1_HUMAN 1 0.162 369 (43) 174 (41) 1500.0 92 Fibrillin-2 FBN2_HUMAN 1 0.136 25 (4)205.9 92 Serum albumin ALBU_HUMAN 0.4605 0.185 6 (5) 12 (4) 81.0 32 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.227 0.325 92 (18) 36 (17) 271.9 44 Lamin-A/C LMNA_HUMAN 0.0585 0.101 20 (7) 15 (7) 122.1 32 Stanniocalcin-2 STC2_HUMAN 0.0585 0.307 16 (7) 16 (7) 124.9 36 Perlecan PGBM_HUMAN 0 0.191 114 (79) 87 (66) 716.4 32 Histone H4. H4_HUMAN 0 0.180 36 (5) 32 (5) 266.9 32 Collagen alpha-2(IV) chain CO4A2_HUMAN 0 0.020 29 (12) 16 (9) 139.8 12 Collagen alpha-1(IV) chain CO4A1_HUMAN 0 0.023 29 (8) 15 (7) 120.1 24 BigH3 BGH3_HUMAN 0 0.093 25 (17) 22 (15) 181.3 32 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0 0.081 21 (10) 15 (10) 120.1 24 Histone H2B H2B1M_HUMAN 0 0.057 8 (4) 29 108.5 16 Calsyntenin-1 CSTN1_HUMAN 0 0.037 8 (8) 8 (8) 77.8 8 Myosin-9 MYH9_HUMAN 0 0.024 7 (7) 7 (6) 55.1 8 Calsyntenin-2 CSTN2_HUMAN 0 0.023 7 (6) 5 (5) 41.5 8 Thrombospondin-1 TSP1_HUMAN 0 0.074 6 (6) 6 (6) 48.9 8 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 6 (6) 8 Fibulin-1 FBLN1_HUMAN 0 0.019 5 (5) 5 (6) 31.4 8 Keratin type I cytoskeletal 14 K1C14_HUMAN 0 0.126 5 (5) 5 (3) 43.6 16 Dedicator of cytokinesis protein 2 DOCK2_HUMAN 0 0.009 4 (2) 8 Protein S100-A7 SA7L1_HUMAN 0 0.037 3 8 46.5 8 Protein S100-A7 S10A7_HUMAN 0 0.037 3 (3) 8 (6) 46.5 8 Insulin-like growth factor-binding protein 3 IBP3_HUMAN 0 0.052 3 (3) 4 (3) 30.3 12

ATP-binding cassette sub-family A member 13 ABCAD_HUMAN 0 0.009 3 (3) 8 Bullous pemphigoid antigen 1 isoforms 1/2/3/4/5/8 BPA1_HUMAN 0 0.009 3 (3) 8 Heterogeneous nuclear ribonucleoprotein M HNRPM_HUMAN 0 0.009 3 (2) 8

Vacuolar protein sorting-associated protein 13A VP13A_HUMAN 0 0.009 3 (2) 8 Complement C1q C1QT3_HUMAN 0 0.036 2 (2) 3 (2) 29.3 12 Insulin-like growth factor-binding protein 4 IBP4_HUMAN 0 0.045 2 (2) 3 (3) 27.3 8 Protein S100-A9 S10A9_HUMAN 0 0.037 2 (2) 3 (3) 24.1 8 Complement factor D CFAD_HUMAN 0 0.009 2 (2) 2 (2) 18.0 8 Fibronectin FINC_HUMAN 0 0.190 2 (2) 2 (2) 18.1 8 Histone H2A type 2-A H2A2A_HUMAN,H2A 0 0.017 2 (2) 2 14.5 12 Cathepsin H CATH_HUMAN 0 0.009 2 (2) 1 (1) 8.0 8 A-kinase anchor protein 9 AKAP9_HUMAN 0 0.009 2 (2) 8 Bile salt export pump ABCBB_HUMAN 0 0.009 2 (2) 8

Bullous pemphigoid antigen 1 isoforms 6/9/10 BPAEA_HUMAN 0 0.009 2 (2) 8 Cadherin-related tumor suppressor homolog FAT1_HUMAN 0 0.009 2 (2) 8 Histone H3 H33_HUMAN 0 0.023 2 (2) 8 Tyrosine-protein kinase BTK BTK_HUMAN 0 0.009 2 (2) 8 Uncharacterized protein C6orf174 CF174_HUMAN 0 0.009 2 (2) 8 Supplemental Table 3 9 Defining the elastic fiber interactome Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score Vacuolar protein sorting-associated protein VP13D_HUMAN 0 0.015 2 (2) 8 Histone H1 H12_HUMAN 0 0.016 4 (2)16.8 8 Keratin type I cytoskeletal 13 K1C13_HUMAN 0 0.112 3 (2)30.2 8 Connective tissue growth factor CTGF_HUMAN 0 0.016 2 (2)18.3 8

PF11 Fibrillin-1 FBN1_HUMAN 1 0.162 131 (32) 50 (21) 416.7 89 Stanniocalcin-2 STC2_HUMAN 0.649 0.307 11 (5) 4 (2) 33.9 56 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.5025 0.325 37 (15) 17 (9) 125.3 56 Histone H4. H4_HUMAN 0.111 0.180 4 (2) 8 (2) 68.2 33 Agrin AGRIN_HUMAN 0.023 0.070 4 (4) 40 BigH3 BGH3_HUMAN 0.023 0.106 4 (4) 40 Perlecan PGBM_HUMAN 0.0115 0.191 4 (4) 2 (2) 7.1 33 Collagen alpha-1(XII) chain COCA1_HUMAN 0 0.009 9 (8) 9 (9) 78.0 22 Insulin-like growth factor-binding protein 3 IBP3_HUMAN 0 0.057 5 (4) 20 Granulins (Proepithelin) GRN_HUMAN 0 0.044 4 (4) 5 (5) 30.5 22 LTBP-2 LTBP2_HUMAN 0 0.030 4 (4) 1 (1) 9.9 22 Insulin-like growth factor-binding protein 7 IBP7_HUMAN 0 0.048 3 (3) 3 (3) 27.0 22 Thrombospondin-1 TSP1_HUMAN 0 0.074 3 (3) 3 (3) 15.9 22 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 3 (3) 20 Keratin type II cytoskeletal 5 K2C5_HUMAN 0 0.095 2 (3) 3 (2) 24.5 22 Protein NOV homolog NOV_HUMAN 0 0.051 2 (2) 3 (3) 18.6 22 Collagen alpha-1(VIII) chain CO8A1_HUMAN 0 0.024 2 (2) 2 (2) 10.1 22 Protein S100-A7 S10A7_HUMAN 0 0.037 2 (2) 2 (2) 9.8 22

PF12 Fibrillin-1 FBN1_HUMAN 0.9975 0.162 84 (35) 48 (29) 355.4 64 Keratin type II cytoskeletal 5 K2C5_HUMAN 0.995 0.158 7 (2)45.2 57 Stanniocalcin-2 STC2_HUMAN 0.4975 0.307 3 (3) 4 (2) 37.4 36 Keratin type II cytoskeletal 6 K2C6A_HUMAN 0.4975 0.112 2 (2) 10 (4) 56.4 36 Granulins (Proepithelin) GRN_HUMAN 0.071 0.044 8 (5) 11 (7) 74.0 43 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.0185 0.325 13 (7) 12 (6) 80.1 43 Histone H4. H4_HUMAN 0 0.180 14 (3) 12 (3) 80.8 29 TGF beta-2 TGFB2_HUMAN 0 0.033 5 (4) 3 (3) 21.2 29 Histone H2B H2B1M_HUMAN 0 0.057 4 (2) 45 (2) 217.8 29 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0 0.081 4 (4) 5 (3) 24.2 14 Insulin-like growth factor-binding protein 7 IBP7_HUMAN 0 0.048 4 (3) 2 (2) 16.4 21 Histone H2A type 2-A H2A2A_HUMAN 0 0.025 4 (2) 29 Keratin type I cytoskeletal 14 K1C14_HUMAN 0 0.126 3 (3) 4 (3) 22.7 21 Actin cytoplasmic 1 ACTB_HUMAN,ACT 0 0.048 3 (3) 3 18.2 21 Matrilin-2 MATN2_HUMAN 0 0.012 3 (3) 2 (2) 16.8 14 Protein NOV homolog NOV_HUMAN 0 0.051 3 (3) 2 (2) 7.5 14 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 3 (3) 14 Retinoic acid receptor responder protein 2 RARR2_HUMAN 0 0.052 2 (2) 6 (3) 16.8 14 Myosin-9 MYH9_HUMAN 0 0.024 2 (2) 3 (3) 23.7 21 BigH3 BGH3_HUMAN 0 0.093 2 (2) 2 (2) 15.5 14 Protein disulfide isomerase A6 PDIA6_HUMAN 0 0.009 2 (2) 2 (2) 16.6 14 Histone H3 H33_HUMAN 0 0.023 2 (2) 14 Keratin type I cytoskeletal K1C16_HUMAN 0 0.131 4 22.7 29 Actin aortic smooth muscle (Alpha-actin-2) ACTA_HUMAN 0 0.058 3 (2)18.2 29 Agrin AGRIN_HUMAN 0 0.078 3 (2)22.1 29 Natriuretic peptides B ANFB_HUMAN 0 0.015 2 (2)16.5 14

Supplemental Table 3 10 Defining the elastic fiber interactome Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score PF13 Fibrillin-1 FBN1_HUMAN 1 0.162 185 (53) 101 (39) 869.8 100 Fibrillin-2 FBN2_HUMAN 0.4975 0.069 2 (2) 9 (4) 87.6 38 Thrombospondin-1 TSP1_HUMAN 0 0.074 29 (19) 31 (22) 239.1 23 Fibronectin FINC_HUMAN 0 0.190 23 (23) 22 (22) 136.4 15 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 20 (17) 17 Agrin AGRIN_HUMAN 0 0.074 16 (15) 17 (16) 123.8 23 Perlecan PGBM_HUMAN 0 0.191 13 (13) 13 (13) 98.6 15 BigH3 BGH3_HUMAN 0 0.093 10 (10) 11 (11) 79.8 15 Plasminogen activator inhibitor 1 PAI1_HUMAN 0 0.325 8 (8) 7 (7) 59.6 15 Extracellular matrix protein 1 ECM1_HUMAN 0 0.012 8 (7) 6 (6) 54.6 15 Protein S100-A7 S10A7_HUMAN 0 0.037 7 (4) 14 (4) 83.6 15 Insulin-like growth factor-binding protein 7 IBP7_HUMAN 0 0.048 7 (6) 6 (5) 50.4 15 Fibrillin-3 FBN3_HUMAN 0 0.006 6 (2) 3 (2) 27.0 23 Collagen alpha-2(I) chain CO1A2_HUMAN 0 0.064 6 (6) 17 Follistatin-related protein FSTL1_HUMAN 0 0.009 5 (5) 3 (3) 29.5 15 TGF beta-2 TGFB2_HUMAN 0 0.033 4 (4) 5 (4) 31.2 15 Insulin-like growth factor-binding protein 4 IBP4_HUMAN 0 0.045 4 (3) 3 (3) 16.7 15 Protein NOV homolog NOV_HUMAN 0 0.051 4 (4) 2 (2) 21.1 15 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0 0.081 3 (3) 5 (5) 25.7 15 Collagen alpha-1(XI) chain COBA1_HUMAN 0 0.013 3 (3) 3 (3) 22.7 15 Keratin type II cytoskeletal 5 K2C5_HUMAN 0 0.095 3 (3) 3 (2) 30.2 15 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.120 3 (3) 17 Protocadherin-17 PCD17_HUMAN 0 0.002 3 (2) 17 Complement C3 CO3_HUMAN 0 0.006 2 (2) 8 (3) 57.6 15 Fibulin-3 FBLN3_HUMAN 0 0.006 2 (2) 2 (2) 20.5 15 Hemoglobin subunit alpha HBA_HUMAN 0 0.006 2 (2) 2 (2) 16.7 15 Plexin domain-containing protein 2 PXDC2_HUMAN 0 0.012 2 (2) 2 (2) 15.5 15 Coiled-coil domain-containing protein CCD80_HUMAN 0 0.009 2 (2) 17 Ryanodine receptor 3 RYR3_HUMAN 0 0.002 2 (2) 17 Insulin-like growth factor-binding protein 5 IBP5_HUMAN 0 0.044 4 (2)29.7 29 Hemoglobin subunit beta HBB_HUMAN 0 0.015 2 (2)14.7 14 Matrilin-2 MATN2_HUMAN 0 0.015 2 (2) 8.1 14 1 alpha chain TPM1_HUMAN 0 0.015 2 (2)15.5 14

PF2 Fibrillin-1 FBN1_HUMAN 1 0.162 216 (55) 142 (58) 1146.9 100 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.9825 0.325 68 (19) 49 (17) 355.5 57 BigH3 BGH3_HUMAN 0.102 0.093 35 (20) 34 (19) 246.9 43 Keratin type I cytoskeletal 14 K1C14_HUMAN 0.102 0.126 16 (9) 17 (7) 142.6 43 Stanniocalcin-2 STC2_HUMAN 0.102 0.307 6 (3) 7 (3) 50.9 43 Thrombospondin-1 TSP1_HUMAN 0.051 0.074 41 (26) 38 (26) 273.0 36 Perlecan PGBM_HUMAN 0.051 0.191 28 (23) 26 (22) 177.8 36 Insulin-like growth factor-binding protein 3 IBP3_HUMAN 0.051 0.052 7 (4) 6 (3) 38.5 36 Protein S100-A7 S10A7_HUMAN 0.051 0.037 2 (2) 10 (2) 85.2 29 LOXL LOXL1_HUMAN 0.001 0.006 2 (2) 2 (1) 17.8 21 Sulfhydryl oxidase 1 QSOX1_HUMAN 0 0.047 25 (20) 29 Fibronectin FINC_HUMAN 0 0.190 19 (16) 20 (17) 90.4 29 Keratin type II cytoskeletal 6 K2C6A_HUMAN 0 0.112 18 (12) 24 (7) 216.1 29 Agrin AGRIN_HUMAN 0 0.074 14 (14) 17 (17) 90.1 14 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.112 14 (13) 2 (2) 16.7 14 Extracellular matrix protein 1 ECM1_HUMAN 0 0.012 12 (10) 9 (8) 79.2 14 Collagen alpha-2(I) chain CO1A2_HUMAN 0 0.061 12 (11) 4 (4) 14.9 14 Keratin type I cytoskeletal K1C16_HUMAN 0 0.070 10 (10) 14 (5) 128.0 21 TGF beta-2 TGFB2_HUMAN 0 0.033 10 (7) 10 (6) 69.6 21

Supplemental Table 3 11 Defining the elastic fiber interactome Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score Insulin-like growth factor-binding protein 7 IBP7_HUMAN 0 0.048 9 (6) 9 (6) 65.8 29 Insulin-like growth factor-binding protein 4 IBP4_HUMAN 0 0.045 7 (3) 5 (3) 42.8 14 Calsyntenin-2 CSTN2_HUMAN 0 0.023 7 (7) 3 (3) 23.0 29 Titin TITIN_HUMAN 0 0.037 6 (6) 14 Protein NOV homolog NOV_HUMAN 0 0.051 5 (5) 4 (4) 29.3 14 Retinoic acid receptor responder protein 2 RARR2_HUMAN 0 0.052 4 (3) 10 (3) 86.3 29 Follistatin-related protein FSTL1_HUMAN 0 0.009 3 (3) 8 (7) 56.0 21 Amyloid A4_HUMAN 0 0.009 3 (3) 5 (4) 14.5 14 Insulin-like growth factor-binding protein 5 IBP5_HUMAN 0 0.034 2 (2) 5 (2) 23.0 21 Calsyntenin-1 CSTN1_HUMAN 0 0.037 2 (2) 3 (3) 24.6 14 Plexin domain-containing protein 2 PXDC2_HUMAN 0 0.012 2 (2) 2 (2) 15.5 14 Protein CYR61 CYR61_HUMAN 0 0.019 2 (2) 2 (2) 7.9 14 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0 0.081 2 (2) 1 (1) 9.1 14 Granulins (Proepithelin) GRN_HUMAN 0 0.044 2 (2) 14 Obscurin OBSCN_HUMAN 0 0.009 2 (2) 14 Keratin type II cytoskeletal 5 K2C5_HUMAN 0 0.158 11 (4)96.7 29 Keratin type I cytoskeletal 13 K1C13_HUMAN 0 0.112 5 (2)49.1 29 Keratin type II cytoskeletal 3 K2C3_HUMAN 0 0.123 5 (2)52.8 29 Bone morphogenetic protein 1 BMP1_HUMAN 0 0.015 4 (3)25.4 14 Fibulin-1 FBLN1_HUMAN 0 0.022 2 (2)16.8 14 Lysyl oxidase LYOX_HUMAN 0 0.016 2 (2)16.2 29

PF5 Fibrillin-1 FBN1_HUMAN 1 0.162 304 (33) 149 (35) 1128.2 100 Histone H4. H4_HUMAN 1 0.180 52 (7) 52 (7) 407.4 100 Lamin-A/C LMNA_HUMAN 1 0.101 46 (19) 44 (19) 342.1 67 Annexin A2 ANXA2_HUMAN 1 0.051 43 (18) 39 (19) 291.8 67 Fibronectin FINC_HUMAN 1 0.190 43 (24) 36 (15) 225.2 67 Stanniocalcin-2 STC2_HUMAN 1 0.307 17 (6) 14 (6) 117.1 67 Annexin V ANXA5_HUMAN 1 0.002 16 (7) 17 (7) 133.8 92 Vimentin VIME_HUMAN 0.001 0.034 18 (12) 18 (10) 146.4 33 Collagen alpha-2(I) chain CO1A2_HUMAN 0.001 0.064 8 (7) 33 Histone H1 H12_HUMAN 0.001 0.016 8 (2)49.0 33 Perlecan PGBM_HUMAN 0.0005 0.191 7 (7) 5 (5) 43.1 25 Insulin-like growth factor-binding protein 5 IBP5_HUMAN 0.0005 0.034 2 (2) 5 (3) 36.6 25 Plasminogen activator inhibitor 1 PAI1_HUMAN 0 0.325 7 (6) 7 (6) 28.3 17 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.112 6 (6) 1 (1) 7.6 17 Secreted frizzled-related protein 4 SFRP4_HUMAN 0 0.012 5 (5) 4 (4) 24.8 17 Growth differentiation factor 15 GDF15_HUMAN 0 0.023 3 (3) 3 (3) 20.9 17 Activated RNA polymerase II TCP4_HUMAN 0 0.002 2 (2) 3 (3) 25.2 17 Coiled-coil domain-containing protein CCD80_HUMAN 0 0.009 2 (2) 17 Histone H3 H33_HUMAN 0 0.023 2 (2) 17 Titin TITIN_HUMAN 0 0.037 2 (2) 17 Histone H1.5 H15_HUMAN 0 0.002 2 (2) 7.2 17

PF7 Fibrillin-1 FBN1_HUMAN 1 0.162 320 (35) 100 (29) 856.7 95 Serum albumin ALBU_HUMAN 0.5 0.1845 19 (5) 14 (5) 113.3 45 Plasminogen activator inhibitor 1 PAI1_HUMAN 0 0.3245 38 (13) 8 (5) 57.7 20 Stanniocalcin-2 STC2_HUMAN 0 0.307 19 (5) 7 (4) 59.5 25 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.112 9 (8) 2 (2) 20.7 15 Insulin-like growth factor-binding protein 4 IBP4_HUMAN 0 0.0445 4 (4) 2 (2) 13.8 15 Retinoic acid receptor responder protein 2 RARR2_HUMAN 0 0.052 3 (3) 4 (2) 36.3 10 Keratin type I cytoskeletal 14 K1C14_HUMAN 0 0.1255 2 (3) 3 (2) 7.5 10 Keratin type II cytoskeletal 5 K2C5_HUMAN 0 0.0945 2 (2) 3 (3) 14.7 10

Supplemental Table 3 12 Defining the elastic fiber interactome Protein Identity SwissProt Entry Average False Scaffold Phenyx Phenyx Reproduc- Name Bayes’ Positive Peptide Peptide Total ibility % Odds rate Count Count Protein Score Elastin ELN_HUMAN 0 0.009 2 (2) 2 (2) 22.7 10 Protein NOV homolog NOV_HUMAN 0 0.051 2 (2) 1 (1) 7.3 10 Perlecan PGBM_HUMAN 0 0.189 2 (2) 10 Probable JmjC domain-containing histone demethylation protein 2C JHD2C_HUMAN 0 0.009 2 (2) 10 BigH3 BGH3_HUMAN 0 0.08 2 (2) 7.3 10

PF8 Fibrillin-1 FBN1_HUMAN 1 0.162 476 (54) 213 (50) 1984.8 100 Fibrillin-2 FBN2_HUMAN 0.915 0.136 10 (2)50.8 50 Plasminogen activator inhibitor 1 PAI1_HUMAN 0.6405 0.325 56 (21) 32 (17) 222.2 50 Stanniocalcin-2 STC2_HUMAN 0.4605 0.307 9 (3) 9 (4) 70.3 44 Fibronectin FINC_HUMAN 0 0.190 19 (19) 16 (16) 112.0 13 Perlecan PGBM_HUMAN 0 0.191 15 (13) 13 (11) 97.1 13 BigH3 BGH3_HUMAN 0 0.093 10 (9) 5 (5) 38.5 19 Collagen alpha-1(XVIII) chain COIA1_HUMAN 0 0.081 9 (8) 7 (5) 55.8 25 Zinc-alpha-2-glycoprotein ZA2G_HUMAN 0 0.012 8 (9) 9 (9) 65.8 13 Insulin-like growth factor-binding protein 3 IBP3_HUMAN 0 0.052 6 (6) 8 (7) 56.0 19 Collagen alpha-1(I) chain CO1A1_HUMAN 0 0.112 5 (4) 1 (1) 7.2 19 Histone H4. H4_HUMAN 0 0.180 4 (2) 4 (2) 32.6 13 Serum albumin ALBU_HUMAN 0 0.185 4 (2) 4 (2) 37.1 25 Keratin type II cytoskeletal 6 K2C6A_HUMAN 0 0.112 3 (5) 4 (1) 22.4 13 Prolactin-inducible protein PIP_HUMAN 0 0.013 3 (3) 3 (3) 26.6 13 Protein S100-A9 S10A9_HUMAN 0 0.037 3 (3) 3 (3) 26.7 13 Apolipoprotein D APOD_HUMAN 0 0.012 3 (3) 2 (2) 15.7 13

Chromodomain-helicase-DNA-binding protein 5 CHD5_HUMAN 0 0.009 3 (2) 13 Histone H2A type 2-A H2A2A_HUMAN,H2A 0 0.025 3 (3) 13 Titin TITIN_HUMAN 0 0.037 3 (3) 13 Amine oxidase AOFA_HUMAN 0 0.006 2 (2) 3 (2) 19.1 19 Agrin AGRIN_HUMAN 0 0.074 2 (2) 2 (2) 7.9 13 Annexin A2 ANXA2_HUMAN 0 0.051 2 (2) 2 (2) 14.7 13 High mobility group protein HMGA1_HUMAN 0 0.012 2 (2) 2 (2) 7.2 13 Collagen alpha-2(I) chain CO1A2_HUMAN 0 0.064 2 (2) 13 Histone H2B H2B1M_HUMAN 0 0.077 2 (2) 13 Keratin type I cytoskeletal 14 K1C14_HUMAN 0 0.092 2 (5) 13 Myosin-9 MYH9_HUMAN 0 0.030 2 (2) 13

Trinucleotide repeat-containing gene 6A protein TNR6A_HUMAN 0 0.009 2 (2) 13 Keratin type II cytoskeletal 5 K2C5_HUMAN 0 0.158 3 (3)22.2 13 Protein CYR61 CYR61_HUMAN 0 0.022 2 (2)14.7 13 Protein S100-A8 S10A8_HUMAN 0 0.043 2 (2)11.2 13 Small breast epithelial mucin MUCL_HUMAN 0 0.015 2 (2) 7.5 13

Proepithelin Granulins (Proepithelin) GRN_HUMAN 1 0.044 72 (19) 43 (16) 408.7 92 Plasminogen activator inhibitor 1 PAI1_HUMAN 0 0.325 5 (5) 4 (4) 30.5 25 Vimentin VIME_HUMAN 0 0.037 2 (2) 17 Serum albumin ALBU_HUMAN 0 0.267 3 (2)22.1 33

Supplemental Table 3 13 Defining the elastic fiber interactome SUPPLEMENTAL TABLE 4 Peptide sequences identified for each proteinfor each cell culture compartment

Scaffold was used to validate protein and peptide identification. Only proteins with >2 unique peptide sequences were counted for each affinity experiment, and each peptide had greater than 95.0% probability as specified by the Peptide Prophet algorithm (38). Shown are the sequences of each peptide, the total number of unique peptide sequences detected in each cell culture compartment along with the total % coverage, and the cell culture compartments in which the identified peptides were detected.

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total Actin cytoplasmic 1 ACTB_HUMAN 14.9 DSYVGDEAQSK 2 1 3 IIAPPER 2 2 AGFAGDDAPR 1 1 GYSFTTTAER 1 1 VAPEEHPVLLTEAPLNPK 1 1 Total 5 3 8

Activated RNA polymerase II TCP4_HUMAN 19.7 EQISDIDDAVR 1 1 EYWMDPEGEMKPGR 1 1 Total 2 2

Agrin AGRIN_HUMAN 12.8 LELGIGPGAATR 4 4 EPLYVGGAPDFSK 3 3 VLGAPVPAFEGR 3 3 AAAVSSGFDGAIQLVSLGGR 2 2 AIVDVHFDPTTAFR 2 2 ALEPQGLLLYNGNAR 2 2 EAAYVCLCPGGFSGPHCEK 2 2 GKDFLALALLDGR 2 2 MALEVVFLAR 2 2 QVDVTSFAGHPCTR 2 2 SFLAFPTLR 2 2 SIESTLDDLFR 2 2 TPSLDAEGSNCPATK 2 2 VLGESPVPHTVLNLK 2 2 VLPEGGAQCECPLGR 2 2 DFLALALLDGR 1 1 EAACLQQTQIEEAR 1 1 FGALCEAETGR 1 1 QLLTPEHVLR 1 1 SPCQPNPCHGAAPCR 1 1 SREPVTLGAWTR 1 1 TDGKGDFVSLALR 1 1 Total 41 41

Supplemental Table 4 14 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total A-kinase anchor protein 9 AKAP9_HUMAN 0.9 ALEIDVEEQVSR 1 1 LQMEAQRICLSLVYSTHVDQVR 1 1 Total 22

Alpha-2-macroglobulin A2MG_HUMAN 2.4 GCVLLSYLNETVTVSASLESVRGNR 1 1 2 QTVSWAVTPK 1 1 2 Total 2 2 4

Amine oxidase AOFA_HUMAN 7.4 TYTIRNEHVDYVDVGGAYVGPTQNR 1 1 WSGYMEGAVEAGER 1 1 Total 22

Amyloid A4_HUMAN 6.2 CLVGEFVSDALLVPDK 1 1 MDVCETHLHWHTVAK 1 1 STNLHDYGMLLPCGIDK 1 1 Total 3 3

Annexin A2 ANXA2_HUMAN 50.7 TNQELQEINR 1 2 3 GLGTDEDSLIEIICSR 2 2 GVDEVTIVNILTNR 2 2 QDIAFAYQR 1 1 2 RAEDGSVIDYELIDQDAR 2 2 SLYYYIQQDTK 2 2 TDLEKDIISDTSGDFR 2 2 TPAQYDASELK 2 2 AYTNFDAERDALNIETAIK 1 1 DALNIETAIK 1 1 LSLEGDHSTPPSAYGSVK 1 1 SALSGHLETVILGLLK 1 1 SYSPYDMLESIR 1 1 SYSPYDMLESIRK 1 1 TDLEKDIISDTSGDFRK 1 1 Total 2 22 24

Annexin V ANXA5_HUMAN 21.9 GLGTDEESILTLLTSR 1 1 2 GTVTDFPGFDER 1 1 2 VLTEIIASR 1 1 2 DLLDDLKSELTGK 1 1 GAGTDDHTLIR 1 1 SEIDLFNIR 1 1 Total 2 4 3 9

Supplemental Table 4 15 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total Apolipoprotein D APOD_HUMAN 16.4 NILTSNNIDVK 1 1 NPNLPPETVDSLK 1 1 VLNQELR 1 1 Total 3 3

ATP-binding cassette sub-family A member 13 ABCAD_HUMAN 1.3 AETYFFSSGGDNLDLTRVLLRKFR 1 1 EIQDLAEEIHGMMDKAKNLK 1 1 NPVLFLAEFFWPCILFVILTVLR 1 1 Total 3 3

BigH3 BGH3_HUMAN 47.0 DLLNNHILK 1 9 10 ILGDPEALR 10 10 SPYQLVLQHSR 1 9 10 LTLLAPLNSVFK 1 8 9 STVISYECCPGYEK 9 9 QAGLGNHLSGSER 2 6 8 DGTPPIDAHTR 7 7 FSMLVAAIQSAGLTETLNR 6 1 7 QHGPNVCAVQK 1 6 7 SLQGDKLEVSLK 7 7 GDELADSALEIFK 6 6 IPSETLNR 1 5 6 VLTPPMGTVMDVLK 6 6 EGVYTVFAPTNEAFR 5 5 YLYHGQTLETLGGK 5 5 YHIGDEILVSGGIGALVR 3 3 DILATNGVIHYIDELLIPDSAK 1 1 2 NSLCIENSCIAAHDK 2 2 TLFELAAESDVSTAIDLFR 2 2 VISTITNNIQQIIEIEDTFETLR 2 2 YGTLFTMDR 2 2

GCPAALPLSNLYETLGVVGSTTTQLYTDR 1 1 NSLCIENSCIAAHDKR 1 1 Total 7 118 2 127

Bile salt export pump ABCBB_HUMAN 3.5 AYAKAGVVADEVISSMRTVAAFGGEK 1 1 DKQALEMVGQITNEALSNIR 1 1 Total 2 2

Bullous pemphigoid antigen 1 isoforms 1/2/3/4/5/8 BPA1_HUMAN 1.5 EVRNLNAELNASK 1 1

Supplemental Table 4 16 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total IQADGFKDQLRSTNEHLHK 1 1 QMEKDLAFQKQVAEK 1 1 Total 3 3

Bullous pemphigoid antigen 1 isoforms 6/9/10 BPAEA_HUMAN 0.9 EKKEAVTSCQEQLDAFQVLVKSLK 1 1 FEEVLAWAKQHQQRLASALAGLIAK 1 1 Total 2 2

Cadherin-related tumor suppressor homolog FAT1_HUMAN 1.1 AEISEFAPPNTPVVMVKAIPAYSHLR 1 1 LYEMEILAADRGMKLYGSSGISSMAK 1 1 Total 2 2

Calsyntenin-1 CSTN1_HUMAN 24.1 AASEFESSEGVFLFPELR 4 1 5 EGLDLQVLEDSGR 3 1 4 ISLSGVHHFAR 3 1 4 LTVTAYDCGK 3 1 4 HHYSLYVHGCR 2 1 3 IPDGVVSVSPK 2 1 3 AMQHISYLNSR 1 1 2 ATVHIQVNDVNEYAPVFK 1 1 2 IHGQNVPFDAVVVDK 1 1 2 KYRPAEFHWK 1 1 2 VIDCLYTCK 1 1 2 DYSFTIQAYDCGK 1 1 ETILCSSDK 1 1 FAESFEVTVTK 1 1 GNLAGLTLR 1 1 GVQIQAHPSQLVLTLEGEDLGELDK 1 1 ISIKPTCTPGWQGWNNR 1 1 QFPTPGIR 1 1 QYDSILR 1 1 Total 27 14 41

Calsyntenin-2 CSTN2_HUMAN 21.4 EGLDINSLESLGQGIK 4 4 VISCLQACK 4 4 SPIDCELQK 3 3 DAPVPFAGEICAFK 2 2 DVNEFAPTFK 2 2 ETILCNSDK 2 2 QECLELNHSELHQR 2 2 QFPTAGVR 2 2 Supplemental Table 4 17 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total AVVHIQVK 1 1 ETILCNSDKTEMNR 1 1 EYTFIIQAYDCGAGPHETAWK 1 1 GTDHFWRPAAQFESAR 1 1 GVTLFPDIK 1 1 LCWVPLLLALGVGSGSGGGGDSRQR 1 1 YEQVLHHIR 1 1 YHFNPSQSILVMEGDDIGNINR 1 1 Total 29 29

Cathepsin H CATH_HUMAN 8.4 MALNQFSDMSFAEIK 1 1 VNHAVLAVGYGEK 1 1 Total 2 2

Chromodomain-helicase-DNA-binding protein 5 CHD5_HUMAN 1.5 AEEKEPIETQQNGDKEEDDEGK 1 1 EPIETQQNGDKEEDDEGKKEDKKGK 1 1 Total 2 2

Coiled-coil domain-containing protein CCD80_HUMAN 2.6 IDHFQLDNEKPMR 1 1 SVFDLIDTFQSR 1 1 Total 2 2

Collagen alpha-1(I) chain CO1A1_HUMAN 25.3 SLSQQIENIR 5 5 VLCDDVICDETK 1 3 4 DGEAGAQGPPGPAGPAGER 1 2 3 GSPGEAGRPGEAGLPGAK 1 2 3 GVQGPPGPAGPR 3 3 ALLLQGSNEIEIR 2 2 DRDLEVDTTLK 2 2 FTYSVTVDGCTSHTGAWGK 2 2 GPPGPMGPPGLAGPPGESGR 2 2 EGAPGAEGSPGR 1 1 GANGAPGIAGAPGFPGAR 1 1 GEPGSPGENGAPGQMGPR 1 1 GETGPAGPAGPVGPVGAR 1 1 GETGPAGRPGEVGPPGPPGPAGEK 1 1 GLTGPIGPPGPAGAPGDKGESGPSGPAGPTGA R 11 GLTGSPGSPGPDGK 1 1 GPAGPQGPR 1 1 GSAGPPGATGFPGAAGR 1 1 NSVAYMDQQTGNLK 1 1 Supplemental Table 4 18 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total STGGISVPGPMGPSGPR 1 1 TGPPGPAGQDGRPGPPGPPGAR 1 1 VGPPGPSGNAGPPGPPGPAGK 1 1 Total 4 35 39

Collagen alpha-1(IV) chain CO4A1_HUMAN 7.5 SAPFIECHGR 4 1 5 AHGQDLGTAGSCLR 3 1 4 HSQTIDDPQCPSGTK 4 4 ILYHGYSLLYVQGNER 2 1 3 FSTMPFLFCNINNVCNFASR 2 2 GTCNYYANAYSFWLATIER 1 1 2 KFSTMPFLFCNINNVCNFASR 1 1

NDYSYWLSTPEPMPMSMAPITGENIRPFISR 1 1 Total 18 4 22

Collagen alpha-1(VIII) chain CO8A1_HUMAN 3.9 GFLDQASGSAVLLLRPGDR 1 1 GGNVWVALFK 1 1 Total 2 2

Collagen alpha-1(XI) chain COBA1_HUMAN 2.9 AAYDYCEHYSPDCDSSAPK 1 1 ALDFHNSPEGISK 1 1 ILDEEVFEGDIQQFLITGDPK 1 1 Total 3 3

Collagen alpha-1(XII) chain COCA1_HUMAN 6.4 GEVQTVTFDTEEVK 2 2 TEFNLNQYYQR 2 2 ALALGALQNIR 1 1 GDTTNTVLQGLK 1 1 GPGDLEAPSNLVISER 1 1 HVFIVDDFESFEK 1 1 ITVIAVYEDGDGGHLTGNGR 1 1 IYIDCYEIIEK 1 1 NNVILQPLQPDTPYK 1 1 SLLQAVANLPYK 1 1 TYQIGWDTFCVK 1 1 VEDIIEAINTFPYR 1 1 VSWTPPSDSVDR 1 1 VYDPSTSTLNVR 1 1 YKVEYYPVSGGK 1 1 Total 17 17

Supplemental Table 4 19 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total Collagen alpha-1(XVIII) chain COIA1_HUMAN 13.0 TEAPSATGQASSLLGGR 5 7 12 LQDLYSIVR 3 5 8 GADFQCFQQAR 3 4 7 AVGLAGTFR 2 3 5 DELLFPSWEALFSGSEGPLKPGAR 3 2 5 EETGAALKPR 1 4 5 LTESYCETWR 3 1 4 GTDNEVAALQPPVVQLHDSNPYPR 3 3 SVWHGSDPNGR 2 1 3 IFSFDGKDVLR 2 2

LSGVQDGHQDISLLYTEPGAGQTHTAASFR 2 2 AAVPIVNLK 1 1 DFQPVLHLVALNSPLSGGMR 1 1 ELLREETGAALKPR 1 1 GERGEPGGGGFFGSSLPGPPGPPGPPGPR 1 1 Total 24 36 60

Collagen alpha-2(I) chain CO1A2_HUMAN 23.4 TGEVGAVGPPGFAGEK 4 4 GETGPSGPVGPAGAVGPR 3 3 TGPPGPSGISGPPGPPGPAGK 3 3 FTYTVLVDGCSK 2 2 SLNNQIETLLTPEGSR 2 2 AVILQGSNDVELVAEGNSR 1 1 GAPGAVGAPGPAGATGDRGEAGAAGPAGPA GPR 11 GDGGPPGMTGFPGAAGR 1 1 GEIGAVGNAGPAGPAGPR 1 1 GEPGSAGPQGPPGPSGEEGK 1 1

GEQGPAGPPGFQGLPGPSGPAGEVGKPGER 1 1 GESGNKGEPGSAGPQGPPGPSGEEGK 1 1 GLHGEFGLPGPAGPR 1 1 GLPGVAGAVGEPGPLGIAGPPGAR 1 1 GPPGAVGSPGVNGAPGEAGR 1 1 GPPGESGAAGPTGPIGSR 1 1 HGNRGETGPSGPVGPAGAVGPR 1 1 VYCDFSTGETCIR 1 1 Total 27 27

Collagen alpha-2(IV) chain CO4A2_HUMAN 16.1 AHNQDLGLAGSCLAR 3 3 ATPFIECNGGR 3 3 SVSIGYLLVK 3 3

Supplemental Table 4 20 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total CSVCEAPAIAIAVHSQDVSIPHCPAGWR 2 2 FSTMPFLYCNPGDVCYYASR 2 2 HSQTDQEPMCPVGMNK 2 2 LWSGYSLLYFEGQEK 2 2 SYWLSTTAPLPMMPVAEDEIKPYISR 2 2

GDPGQHGLPGFPGLKGVPGNIGAPGPKGAK 1 1 GFPGSPGSDIHGDPGFPGPPGER 1 1 GPRGEQGFMGNTGPTGAVGDRGPK 1 1 SLWIGYSFLMHTAAGDEGGGQSLVSPGSCLE DFR 1 1 YSFWLTTIPEQSFQGSPSADTLK 1 1 Total 24 24

Collagen alpha-3(IV) chain CO4A3_HUMAN 3.8 ASPFLECHGR 1 1 CIEGPRGAQGLPGLNGLKGQQGR 1 1

GFTGPEGLPGPQGPKGFPGLPGLTGSKGVR 1 1 Total 3 3

Collagen alpha-3(V) chain CO5A3_HUMAN 3.4 GEKGEDGFPGFKGDVGLKGDQGKPGAPGPR 1 1

TGEVGPLGERGPPGPPGPPGEQGLPGLEGR 1 1 Total 22

Complement C1q C1QT3_HUMAN 12.2 FGAPVSGVYFFTFSMMK 1 1 FSTFAGFLLFETK 1 1 Total 2 2

Complement C3 CO3_HUMAN 1.4 ACEPGVDYVYK 1 1 TIYTPGSTVLYR 1 1 Total 2 2

Complement factor D CFAD_HUMAN 19.0 AVPHPDSQPDTIDHDLLLLQLSEK 1 1 VDRDVAPGTLCDVAGWGIVNHAGR 1 1 Total 22

Dedicator of cytokinesis protein 2 DOCK2_HUMAN 2.4 EDGTTLHDGFHDLVVLKGDSK 1 1 GEQCEPILRTLKALEYVFKFIVR 1 1 Total 2 2

Dickkopf-related protein 1 DKK1_HUMAN 7.9 GQEGSVCLR 1 1

Supplemental Table 4 21 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total SSDCASGLCCAR 1 1 Total 22

Elastin ELN_HUMAN 4.2 AGYPTGTGVGPQAAAAAAAK 1 1 LPGGYGLPYTTGK 1 1 Total 2 2

Extracellular matrix protein 1 ECM1_HUMAN 29.1 AWEDTLDKYCDR 2 2 ELLALIQLER 2 2 FSCFQEEAPQPHYQLR 2 2 LDGFPPGRPSPDNLNQICLPNR 2 2 QGETLNFLEIGYSR 2 2 VTPNLMGHLCGNQR 2 2 ACPSHQPDISSGLELPFPPGVPTLDNIK 1 1 ELPSLQHPNEQK 1 1 FCEAEFSVK 1 1 LTFINDLCGPR 1 1 LVWEEAMSR 1 1 Total 17 17

Fibrillin-1 FBN1_HUMAN 65.8 YEDEECTLPIAGR 7 16 10 13 46 NTPEYEELCPR 8 13 11 12 44 CSNQLPQSITK 6 17 5 9 37 CWSPGVTVAPEMCPIR 5 15 2 8 30 TLPGGNQCIVPICR 7 13 6 4 30 CECEIGYELDR 683724 CLCYDGFMASEDMK 563822 GYIGTHCGQPVCESGCLNGGR 5 10 3 4 22 CPPGFYTSPDGTR 5 10 1 5 21 GQCVNTPGDFECK 546621 GTCWQTVIDGR 396321 ISPDLCGR 457521 LETCFLR 566421 YNAYCCPGWK 582621 CSCSPGWIGDGIK 573520 CACTYGFTGPQCER 492419 TGCTDINECEIGAHNCGK 652619 CDSGFALDSEER 426618 GDNGDTACSNEIGVGVSK 6 7 5 18 MIPSLCTHGK 437418 CECDMGFVPSADGK 451717 YQCACNPGYHSTPDR 247417

Supplemental Table 4 22 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total AWGHPCEMCPAQPHPCR 472316 CECFPGLAVGLDGR 2 10 1 3 16 GGGGHDALKGPNVCGSR 462416 CDEGYESGFMMMK 234615 CQCPSGMTLDATGR 353415 GFMTNGADIDECK 172515 SGNCYLDIRPR 542415 CICNSGYEVDSTGK 343414 TCVDINECLLEPR 1 8 5 14 CIDVRPGYCYTALTNGR 452213 GFIYKPDLK 443213 GQCIKPLFGAVTK 2 8 3 13 CLCPEGFSLSSSGR 2 7 3 12 EITNGKPFFK 325212 NCMDIDECQR 235212 NCMDIDECQRDPLLCR 234312 RGFIPNIR 2 7 3 12 TCVDVNECDLNPNICLSGTCENTK 431412 TGPCFTVISNQMCQGQLSGIVCTK 171312 ACEDIDECSLPNICVFGTCHNLPGLFR 421411 CICPPGYSLQNEK 2 6 3 11 GWGPHCEICPFQGTVAFK 5 6 11 MDACCCSVGAAWGTEECEECPMR 314311 SIQHCNIR 323210 TNDDQTMCLDINECER 1 5 4 10 CECPVGFFYNDK 1 6 2 9 CICKPGFQLASDGR 1 5 3 9 GGVCHNTEGSYR 21429 GTQCEDIDECEVFPGVCK 13329 AGYQSTLTR 1 6 1 8 NCEDMDECSIR 7 1 8 SCTDIDECEDNPNICDGGQCTNIPGEYR 22318 VCVDTHMR 7 1 8 CEININGATLK 12137 CPTGYYLNEDTR 1 3 3 7 DINECETPGICMNGR 4 3 7 GEGWGDPCELCPTEPDEAFR 1 5 1 7 GSFICHCDMGYSGK 3 2 2 7 GYILQEDGR 2237 IGQGHCVSGMGMGR 3 4 7 AWGTPCEMCPAVNTSEYK 2 2 2 6 CIPTPGSYR 5 1 6

Supplemental Table 4 23 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total EGYCFTEVLQNMCQIGSSNR 2 4 6 HGQCINTDGSYR 1 3 2 6 LLVCEDIDECQNGPVCQR 1 3 2 6 CINTFGSFQCR 1 3 1 5 CQHGCQNIIGGYR 2 3 5 EIPGVCENGVCINMVGSFR 4 1 5 GICQNTPGSFTCECQR 2 3 5 NCVDINECVLNSLLCDNGQCR 1 1 3 5 NTPGSFVCTCPK 1 3 1 5 CPPGFTQHHTSCIDNNECTSDINLCGSK 2 2 4 CPVGYVLR 1 2 1 4 DIDECLQNGR 3 1 4 GFSLDQTGSSCEDVDECEGNHR 1 3 4 GSYHCICK 1 3 4 HAVCTNTAGSFK 2 2 4 HSCGDGFCSRPNMCTCPSGQIAPSCGSR 3 1 4 IKGTQCEDIDECEVFPGVCK 3 1 4 KLCPHGR 2 2 4 NSPGSFICECSSESTLDPTK 1 2 1 4 QHNCQFLCVNTIGGFTCK 1 3 4 RGGGGHDALKGPNVCGSR 2114 RPDGEGCVDENECQTKPGICENGR 1 3 4 SQCCSSLGAAWGSPCTLCQVDPICGK 2114 CTDLDECSNGTHMCSQHADCK 1 1 1 3 CVNTDGSYR 3 3 CVNTYGSYECK 2 1 3 KCAPGTCQNLDGSYR 3 3 MCKDEDECEEGKHDCTEK 2 1 3 NGLCVNTR 1 2 3 NLIGTYMCICGPGYQR 2 1 3 NMCLNGMCINEDGSFK 2 1 3 NTEGSYQCSCPK 1 2 3 QECCCALK 2 1 3 RGQCIKPLFGAVTK 2 1 3

TGYTPDITGTSCVDLNECNQAPKPCNFICK 2 1 3 VLPVNVTDYCQLVR 1 2 3 ASCCCSLGK 1 1 2 CAPGTCQNLDGSYR 1 1 2 CEDIDECSTIPGICEGGECTNTVSSYFCK 1 1 2 CINTDGSFHCVCNAGFHVTR 2 2 CRCDSGFALDSEER 2 2

Supplemental Table 4 24 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total DEDECEEGKHDCTEK 1 1 2 DGKNCEDMDECSIR 1 1 2 EGYCFTEVLQNMCQIGSSNRNPVTK 2 2 GFMTNGADIDECKVIHDVCR 2 2 GFQLDLR 1 1 2 GKTGCTDINECEIGAHNCGK 1 1 2 GNPEPPVSGEMDDNSLSPEACYECK 2 2 GSFKCQCPSGMTLDATGR 1 1 2 GWGPHCEICPFQGTVAFKK 2 2 ICLDIR 1 1 2 ILELLPALTTLTNHNR 2 2 NCTDIDECR 1 1 2 QICPYGSGIIVGPDDSAVDMDECK 1 1 2

QICPYGSGIIVGPDDSAVDMDECKEPDVCK 1 1 2 SCKDLDECATK 2 2 TCEDIDECESSPCINGVCK 1 1 2 YLIESGNEDGFFK 2 2 CINTFGSFQCRCPTGYYLNEDTR 1 1 CRCDSGFALDSEERNCTDIDECR 1 1 CRNTIGSFK 1 1 CWSPGVTVAPEMCPIRATEDFNK 1 1 DPLLCR 1 1 EGYTGDGFTCTDLDECSENLNLCGNGQCLNA PGGYR 11 FTSTGQCNDR 1 1 GECIDVDECEK 1 1 GSFICHCDMGYSGKK 1 1 GSYTCECNDGFTASPNQDECLDNR 1 1 GTQCEDIDECEVFPGVCKNGLCVNTR 1 1 ILCPGGEGFRPNPITVILEDIDECQELPGLCQG GK 1 1 KKPVAGTYSLQISSTPLYK 1 1 KMCCCSYNIGR 1 1 MCCCSYNIGR 1 1 MQCCCDAGR 1 1 MSYCYAK 1 1 NAECINTAGSYR 1 1 NGQCINTVGSFQCQCNEGYEVAPDGR 1 1 NTIGSFNCR 1 1 SECCCDGGRGWGPHCEICPFQGTVAFK 1 1 VGCVDTR 1 1 VIHDVCRNGECVNDR 1 1

Supplemental Table 4 25 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total Total 211 457 183 338 1189

Fibrillin-2 FBN2_HUMAN 0.8 CLCNEGYELTPDGK 1 1 LSSTGLICIDSLK 1 1 Total 2 2

Fibrillin-3 FBN3_HUMAN 0.8 CQHGCQNQLGGYR 1 1 GYLLEEDGR 1 1 Total 22

Fibronectin FINC_HUMAN 30.6 SYTITGLQPGTDYK 4217 SSPVVIDASTAIDAPSNLR 3126 ISCTIANR 12115 VPGTSTSATLTGLTR 2125 DLQFVEVTDVK 2114 EINLAPDSSSVVVSGLMVATK 2114 GEWTCIAYSQLR 2 2 4 IYLYTLNDNAR 2 2 4 NLQPASEYTVSLVAIK 3 1 4 WLPSSSPVTGYR 3 1 4 FLATTPNSLLVSWQPPR 1113 NTFAEVTGLSPGVTYYFK 2 1 3 VDVIPVNLPGEHGQR 1113 DSMIWDCTCIGAGR 1 1 2 GATYNIIVEALK 1 1 2 HTSVQTTSSGSGPFTDVR 1 1 2 ITYGETGGNSPVQEFTVPGSK 2 2 QYNVGPSVSK 1 1 2 RPGGEPSPEGTTGQSYNQYSQR 1 1 2 TFYSCTTEGR 2 2 TYLGNALVCTCYGGSR 1 1 2 VGDTYERPK 1 1 2 VTIMWTPPESAVTGYR 1 1 2 WSRPQAPITGYR 2 2 DDKESVPISDTIIPAVPPPTDLR 1 1 EATIPGHLNSYTIK 1 1 EESPLLIGQQSTVSDVPR 1 1 ESKPLTAQQTTK 1 1 EVTSDSGSIVVSGLTPGVEYVYTIQVLR 1 1 FTNIGPDTMR 1 1 FTQVTPTSLSAQWTPPNVQLTGYR 1 1 GATYNIIVEALKDQQR 1 1

Supplemental Table 4 26 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total GDSPASSKPISINYR 1 1 GFNCESKPEAEETCFDK 1 1 GLAFTDVDVDSIK 1 1 GLKPGVVYEGQLISIQQYGHQEVTR 1 1 IAWESPQGQVSR 1 1 IGDQWDKQHDMGHMMR 1 1 LLCQCLGFGSGHFR 1 1 SDTVPSPR 1 1 TEIDKPSQMQVTDVQDNSISVK 1 1 TETITGFQVDAVPANGQTPIQR 1 1 TGLDSPTGIDFSDITANSFTVHWIAPR 1 1 TIKPDVR 1 1 TKTETITGFQVDAVPANGQTPIQR 1 1 VTDATETTITISWR 1 1 VTWAPPPSIDLTNFLVR 1 1 YEKPGSPPR 1 1 YEVSVYALK 1 1 Total 2 56 26 19 103

Fibulin-1 FBLN1_HUMAN 8.1 DCSLPYATESK 2 2 TGYYFDGISR 2 2 CCHCCLLGR 1 1 GYHLNEEGTR 1 1 SQETGDLDVGGLQETDK 1 1 Total 77

Fibulin-3 FBLN3_HUMAN 6.5 EHIVDLEMLTVSSIGTFR 1 1 NPCQDPYILTPENR 1 1 Total 2 2

Follistatin-related protein FSTL1_HUMAN 31.5 CALEDETYADGAETEVDCNR 2 2 ICANVFCGAGR 2 2 CVCACGNWVCTAMTCDGK 1 1 ECAVTEKGEPTCLCIEQCKPHK 1 1 GLCVDALIELSDENADWK 1 1 LSFQEFLK 1 1 Total 8 8

Golgin subfamily B member 1 GOGB1_HUMAN 1.3 ILALEKEKEQLQKKLQEALTSRK 1 1 REKVHLEETIGEIQVTLNK 1 1 Total 22

Supplemental Table 4 27 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total Hemoglobin subunit alpha HBA_HUMAN TYFPHFDLSHGSAQVK 1 1 VGAHAGEYGAEALER 1 1 Total 2 2

Heterogeneous nuclear ribonucleoprotein M HNRPM_HUMAN 5.9 FGSGMNMGRINEILSNALKR 1 1 GIGMGNIGPAGMGMEGIGFGINK 1 1 Total 2 2

High mobility group protein HMGA1_HUMAN 23.4 EPSEVPTPK 1 1 KQPPVSPGTALVGSQK 1 1 Total 22

Histone H2A type 2-A H2A2A_HUMAN 67.7 AGLQFPVGR 5 3 1 9 HLQLAIR 4 1 1 6 VTIAQGGVLPNIQAVLLPK 1 1 2 ARAKAKSRSSRAGLQFPVGR 1 1 LLRKGNYAERVGAGAPVYMAAVLEYLTAEI LELAGNAARDNK 1 1 Total 11 6 2 19

Histone H2B H2B1M_HUMAN 52.4 LLLPGELAK 7 1 8 QVHPDTGISSK 4 1 1 6 AMGIMNSFVNDIFER 3 1 4 ESYSVYVYK 1 1 KESYSVYVYK 1 1 MPEPVKSAPVPKKGSKKAINK 1 1 Total 16 3 2 21

Histone H3 H33_HUMAN 16.2 YRPGTVALR 1 1 1 3 KLPFQR 1 1 2 STELLIR 1 1 Total 2 2 2 6 Total 222 6 Histone H4. H4_HUMAN 52.4 ISGLIYEETR 18 4 8 4 34 DNIQGITKPAIR 12 4 8 4 28 VFLENVIR 12 4 6 22 DAVTYTEHAK 642214 TVTAMDVVYALK 624214 KTVTAMDVVYALK 4 2 6 TVTAMDVVYALKR 2 2 4 Total 60 18 32 12 122

Supplemental Table 4 28 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total

Insulin-like growth factor II IGF2_HUMAN 13.9 GIVEECCFR 1 1 SCDLALLETYCATPAK 1 1 Total 22

Insulin-like growth factor-binding protein 3 IBP3_HUMAN 24.4 ALAQCAPPPAVCAELVR 5 2 7 YGQPLPGYTTK 6 1 7 FLNVLSPR 3 3 EMEDTLNHLK 1 1 2 GVHIPNCDKK 2 2 SAGSVESPSVSSTHR 2 2 GVHIPNCDK 1 1 Total 20 4 24

Insulin-like growth factor-binding protein 4 IBP4_HUMAN 31.0 GELDCHQLADSFRE 1 7 8 CRPPVGCEELVR 7 7 THEDLYIIPIPNCDR 1 4 5 EDARPVPQGSCQSELHR 4 4 GELDCHQLADSFR 1 1 QCHPALDGQR 1 1 TGVKLPGGLEPKGELDCHQLADSFR 1 1 Total 2 25 27

Insulin-like growth factor-binding protein 5 IBP5_HUMAN 18.0 ALSMCPPSPLGCELVK 2 2 QDEEKPLHALLHGR 2 2 AVYLPNCDR 1 1 HMEASLQELK 1 1 Total 66

Insulin-like growth factor-binding protein 7 IBP7_HUMAN 51.8 AGAAAGGPGVSGVCVCK 1 7 8 ITVVDALHEIPVK 1 7 8 TELLPGDRDNLAIQTR 6 6 GTCEQGPSIVTPPK 5 5 EDAGEYECHASNSQGQASASAK 3 3 HEVTGWVLVSPLSK 3 3 GEGEPCGGGGAGR 1 1 GGPEKHEVTGWVLVSPLSK 1 1 GYCAPGMECVK 1 1 ITVVDALHEIPVKK 1 1

Supplemental Table 4 29 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total SRYPVCGSDGTTYPSGCQLR 1 1 Total 2 36 38

Keratin type I cytoskeletal K1C16_HUMAN 38.5 ALEEANADLEVK 2 2 ASLENSLEETK 2 2 GQTGGDVNVEMDAAPGVDLSR 2 2 IIAATIENAQPILQIDNAR 2 2 TRLEQEIATYR 2 2 APSTYGGGLSVSSR 1 1 TEELNKEVASNSELVQSSR 1 1 TKYEHELALR 1 1 VLDELTLAR 1 1 VLQGLEIELQSQLSMK 1 1 Total 15 15

Keratin type I cytoskeletal 10 K1C10_HUMAN 63.9 QSLEASLAETEGR 9 4 5 12 30 ALEESNYELEGK 10 2 8 9 29 GSLGGGFSSGGFSGGSFSR 926522 LKYENEVALR 516921 LENEIQTYR 614819 IRLENEIQTYR 245617 NVQALEIELQSQLALK 443516 VLDELTLTK 423615 SQYEQLAEQNR 411713 VTMQNLNDR 432413 AETECQNTEYQQLLDIK 412512 SLLEGEGSSGGGGR 512412 QSVEADINGLR 4 7 11 DAEAWFNEK 22239 ELTTEIDNNIEQISSYK 2 2 5 9 SQYEQLAEQNRK 31239 GSSGGGCFGGSSGGYGGLGGFGGGSFR 11237 LASYLDKVR 3 2 2 7 NQILNLTTDNANILLQIDNAR 1326 ADLEMQIESLTEELAYLK 1 4 5 SKELTTEIDNNIEQISSYK 1 3 1 5 ADLEMQIESLTEELAYLKK 1 1 1 3 HYSSSRSGGGGGGGGCGGGGGVSSLR 1 1 2 LAADDFR 2 2 LASYLDK 1 1 2

NVSTGDVNVEMNAAPGVDLTQLLNNMR 1 1 2

Supplemental Table 4 30 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total YENEVALR 2 2 HGNSHQGEPR 1 1 IKEWYEK 1 1 ISSSKGSLGGGFSSGGFSGGSFSR 1 1 NHEEEMKDLR 1 1 QSVEADINGLRR 1 1 SGGGGGGGGCGGGGGVSSLR 1 1 TIDDLKNQILNLTTDNANILLQIDNAR 1 1 YCVQLSQIQAQISALEEQLQQIR 1 1 Total 92 35 63 118 308

Keratin type I cytoskeletal 14 K1C14_HUMAN 38.1 VLDELTLAR 1 2 4 7 ALEEANADLEVK 3 3 6 APSTYGGGLSVSSSR 1225 TKYETELNLR 2 3 5 VTMQNLNDR 1 1 3 5 ASLENSLEETK 2 1 3 EVATNSELVQSGK 1 2 3 GSCGIGGGIGGGSSR 2 2 LASYLDKVR 1 1 2 TRLEQEIATYR 2 2 ADLEMQIESLK 1 1 GQVGGDVNVEMDAAPGVDLSR 1 1 ILNEMRDQYEK 1 1 LAADDFR 1 1 LASYLDK 1 1 TMQNLEIELQSQLSMK 1 1 Total 5 2 17 22 46

Keratin type I cytoskeletal 9 K1C9_HUMAN 47.4 TLLDIDNTR 8 10 9 4 31 VQALEEANNDLENK 469827 SGGGGGGGLGSGGSIR 578323 HGVQELEIELQSQLSK 455317 FSSSSGYGGGSSR 257216 IKFEMEQNLR 414514 QGVDADINGLR 314210 QVLDNLTMEK 2 3 4 9 STMQELNSR 22329 GGSGGSYGGGGSGGGYGGGSGSR 21317 QEIECQNQEYSLLLSIK 1247 QEYEQLIAK 2 3 1 6 TLNDMRQEYEQLIAK 3 1 2 6

Supplemental Table 4 31 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total EIETYHNLLEGGQEDFESSGAGK 2 1 1 4 SDLEMQYETLQEELMALK 2 1 3 LASYLDK 1 1 2 NHKEEMSQLTGQNSGDVNVEINVAPGK 1 1 2 SDLEMQYETLQEELMALKK 2 2 DQIVDLTVGNNK 1 1 IQDWYDKK 1 1 LASYLDKVQALEEANNDLENK 1 1 NYSPYYNTIDDLKDQIVDLTVGNNK 1 1 YCGQLQMIQEQISNLEAQITDVR 1 1 Total 45 45 62 48 200

Keratin type II K22E_HUMAN 45.0 FLEQQNQVLQTK 4 5 9 18 IEISELNR 514818 LNDLEEALQQAK 413816 NVQDAIADAEQR 3 3 5 11 VDLLNQEIEFLK 3 3 4 10 YEELQVTVGR 11237 LALDVEIATYR 2 4 6 NLDLDSIIAEVK 1 2 3 6 SISISVAGGGGGFGAAGGFGGR 1 2 2 5 YEDEINKR 1 1 3 5 GFSSGSAVVSGGSR 1214 TAAENDFVTLKK 1 1 2 4 NKLNDLEEALQQAK 2 1 3 TSQNSELNNMQDLVEDYKK 1 2 3 LLEGEECR 2 2 STSSFSCLSR 1 1 2 YLDGLTAER 1 1 2 AQYEEIAQR 1 1 GGSISGGGYGSGGGK 1 1 GSSSGGGYSSGSSSYGSGGR 1 1 HGGGGGGFGGGGFGSR 1 1 HSSGGGSRGGSSSGGGYGSGGGGSSSVK 1 1 TAAENDFVTLK 1 1 Total 25 7 35 61 128

Keratin type II cuticular Hb1 KRT81_HUMAN 3.4 LLEGEEQR 1 1 LTAEVENAK 1 1 Total 22

Keratin type II cytoskeletal 1 K2C1_HUMAN 49.7 FLEQQNQVLQTK 13 18 16 17 64

Supplemental Table 4 32 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total YEELQITAGR 11 13 13 15 52 QISNLQQSISDAEQR 13 11 11 13 48 LNDLEDALQQAK 11 12 11 12 46 TNAENEFVTIK 10 6 12 13 41 SLDLDSIIAEVK 12 9 9 8 38 SLNNQFASFIDK 988833 IEISELNR 11 3 5 9 28 WELLQQVDTSTR 876526 FSSCGGGGGSFGAGGGFGSR 666624 TNAENEFVTIKK 648523 NMQDMVEDYR 7 1 4 10 22 TLLEGEESR 365519 THNLEPYFESFINNLR 524718 NKLNDLEDALQQAK 335617 LALDLEIATYR 353516 SKAEAESLYQSK 543315 SLNNQFASFIDKVR 523313 AEAESLYQSK 313411 SGGGFSSGSAGIINYQR 31239 MSGECAPNVSVSVSTSHTTISGGGSR 2 2 2 6 SKAEAESLYQSKYEELQITAGR 4 1 1 6 SLVNLGGSKSISISVAR 2136 YEDEINKR 2 1 3 6 DYQELMNTK 1 1 3 5 SLVNLGGSK 1 1 2 4 KQISNLQQSISDAEQR 2 1 3 DVDGAYMTK 1 1 2 GENALKDAK 1 1 GGSGGGGGGSSGGRGSGGGSSGGSIGGR 1 1 KDVDGAYMTK 1 1 NKYEDEINKR 1 1 SEIDNVKK 1 1 SISISVAR 1 1 Total 161 126 147 173 607

Keratin type II cytoskeletal 5 K2C5_HUMAN 33.2 ISISTSGGSFR 1 1 1 3 YEELQQTAGR 1 2 3 LAELEEALQK 2 2 LLEGEECR 2 2 TTAENEFVMLK 2 2 YEDEINKR 2 2 FASFIDKVR 1 1

Supplemental Table 4 33 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total HEISEMNR 1 1 LALDVEIATYR 1 1 NLDLDSIIAEVK 1 1 NMQDLVEDFK 1 1 QCANLQNAIADAEQR 1 1 SFSTASAITPSVSR 1 1 VSLAGACGVGGYGSR 1 1 Total 3 3 16 22

Keratin type II cytoskeletal 6 K2C6A_HUMAN 43.1 ISIGGGSCAISGGYGSR 2 1 3 WTLLQEQGTK 1 1 1 3 ADTLTDEINFLR 2 2 AIGGGLSSVGGGSSTIK 2 2 LALDVEIATYR 2 2 NKLEGLEDALQK 2 2 NLDLDSIIAEVK 1 1 2 QNLEPLFEQYINNLR 1 1 2 TAAENEFVTLK 1 1 2 YEDEINKR 1 1 2 YEELQVTAGR 2 2 AQYEEIAQR 1 1 FLEQQNKVLETK 1 1 GMQDLVEDFK 1 1 GRLDSELR 1 1 GSGGLGGACGGAGFGSR 1 1 LLEGEECR 1 1 NKYEDEINKR 1 1 QCANLQAAIADAEQR 1 1 QEIAEINR 1 1 QLDSIVGER 1 1 TAAENEFVTLKK 1 1 Total 9 18 8 35

Lamin-A/C LMNA_HUMAN 34.5 SNEDQSMGNWQIK 3 6 9 TALINSTGEEVAMR 4 5 9 AGQVVTIWAAGAGATHSPPTDLVWK 3 4 7 VAVEEVDEEGKFVR 2 5 7 QNGDDPLLTYR 1 2 3 AQNTWGCGNSLR 1 1 2 VAVEEVDEEGK 1 1 2 AAYEAELGDAR 1 1

Supplemental Table 4 34 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total IDSLSAQLSQLQK 1 1 LADALQELR 1 1 LKDLEALLNSK 1 1 LQEKEDLQELNDR 1 1 LQTMKEELDFQK 1 1 MQQQLDEYQELLDIK 1 1 NSNLVGAAHEELQQSR 1 1 RQNGDDPLLTYR 1 1 SGAQASSTPLSPTR 1 1 SSFSQHAR 1 1 SVGGSGGGSFGDNLVTR 1 1 Total 16 35 51

LOXL LOXL1_HUMAN 5.9 GLPDLVPDPNYVQASTYVQR 1 1 YIVLESDFTNNVVR 1 1 Total 2 2

LTBP-2 LTBP2_HUMAN 3.5 STPLGQQQPAPR 2 2 EQDAPVAGLQPVER 1 1 RPGGSYPAAAAAK 1 1 SGAAPPTPPR 1 1 SLGPGTCTLPLAQR 1 1 Total 6 6

Lysyl oxidase LYOX_HUMAN 15.3 DPGAAVPGAANASAQQPR 1 1 2 TPILLIR 1 1 2 VDGMVGDDPYNPYK 2 2 CAAEENCLASTAYR 1 1 HWFQAGYSTSR 1 1 Total 3 5 8

MAGP-1 MFAP2_HUMAN 35.5 TVCAHEELLR 1 3 2 6 LYSIHRPCK 3 2 5 EEQYPCTR 1 1 2 4 QCLNEVCFYSLR 2 1 3 RVYVINK 1 2 3 CGVMASSGLCQSVAASCAR 1 1 Total 2 10 10 22

Matrilin-2 MATN2_HUMAN 4.0 ADLVFIIDSSR 1 1 THPQTALLESSCENKR 1 1

Supplemental Table 4 35 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total VGLLQYGSTVK 1 1 Total 3 3

Myosin-9 MYH9_HUMAN 9.5 QLEEAEEEAQR 3 1 1 5 ALEQQVEEMK 2 2 ANLQIDQINTDLNLER 2 2 IAQLEEQLDNETK 1 1 2 LQQELDDLLVDLDHQR 1 1 2 DELADEIANSSGK 1 1 DLEAHIDSANK 1 1 ELEDATETADAMNR 1 1 EQLEEEEEAKHNLEK 1 1 IAQLEEELEEEQGNTELINDR 1 1 LQVELDNVTGLLSQSDSK 1 1 NAEQYKDQADK 1 1 TQLEELEDELQATEDAK 1 1 Total 16 2 3 21

Neutrophil defensin 13 DEF1_HUMAN 19.1 IPACIAGER 1 1 YGTCIYQGR 1 1 Total 22

Obscurin OBSCN_HUMAN 0.6 DGRTLSPGPKYEVQASAGRR 1 1 EGATAVLQCELNSAAPVEWRKGSETLR 1 1 Total 2 2

Perlecan PGBM_HUMAN 36.5 AGLSSGFIGCVR 5 3 3 11 LHHVSPADSGEYVCR 5 4 2 11 LPAVEPTDQAQYLCR 3 6 1 10 LDVEFKPLAPDGVLLFSGGK 4 3 2 9 LHQMSVADSGEYVCR 3 4 2 9 YELGSGLAVLR 3 4 2 9 AAGVPSATITWR 1 7 8 LEGDTLIIPR 2 6 8 ASYAQQPAESR 1 6 7 EVSEAVVDTLESEYLK 7 7 LPQVSPADSGEYVCR 2 5 7 LSGSHSQGVAYPVR 7 7 SPAYTLVWTR 2 4 1 7 AVTLECVSAGEPR 2 4 6 IQVVVLSASDASPPPVK 1 5 6 LLSGPYFWSLPSR 5 1 6

Supplemental Table 4 36 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total SIEYSPQLEDAGSR 6 6 AFAHLQVPER 1 4 5 CLCLPGFSGPR 2 2 1 5 GHTPTQPGALNQR 5 5 GMVFGIPDGVLELVPQR 4 1 5 IAHVELADAGQYR 5 5 LHLVSPADSGEYVCR 1 4 5 LYIFQASPADAGQYVCR 1 4 5 LYQASPADSGEYVCR 1 4 5 VGGHLRPGIVQSGGVVR 1 4 5 VTSYGGELR 2 3 5 ALEVEECR 2 2 4 EGGQLPPGHSVQDGVLR 1 2 1 4 GPSCQDCDTGYTR 2 2 4 IESSSPTVVEGQTLDLNCVVAR 2 2 4 ITFRPDSADGMLLYNGQK 2 1 1 4 RVPGSPTNLANR 1 3 4 SAEPLALGR 2 2 4 SGPVEDFVSLAMVGGHLEFR 1 2 1 4 SIGASVEFHCAVPSDR 2 2 4 SQSVRPGADVTFICTAK 1 3 4 TCESLGAGGYR 1 3 4 TPSGLYLGTCER 2 2 4 AELLVTEAPSKPITVTVEEQR 1 2 3 CLIHDGAAPISLEWK 3 3 DGFKGDLCEHEENPCQLR 2 1 3 EHLLMALAGIDTLLIR 3 3 FDAGSGMATIR 2 1 3 GGSLPSHHQTR 2 1 3 IPGDQVVSVVFIK 3 3 IPQVTPADSGEYVCHVSNGAGSR 3 3 IQNLDQSCQGTYICQAHGPWGK 1 2 3 LCNECADGSFHLSTR 3 3 LGTVPQFPR 1 2 3 LLQVTPADSGEYVCR 1 2 3 MASVGLSDIAMDTTVTHATSHGR 3 3 NELLHFER 1 2 3 RGGSLPAR 1 1 1 3 VAEGQTLDLK 3 3 VDSYGGSLR 3 3 VTVTSEGGR 3 3 VVGSSGTQEASVLVTIQQR 3 3

Supplemental Table 4 37 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total AAGVPSATITWRKEGGSLPPQARSER 1 1 2 AMDFNGILTIR 1 1 2 AQIHGGILR 2 2 ATATSCRPCPCPYIDASR 2 2 AVLHVHGGGGPR 1 1 2

CEEGVTVTTPSLSGAGSYLALPALTNTHHELR 1 1 2 CRPVNQEIVR 1 1 2 CSATGSPAPTIHWSK 2 2 EGGSLPPQAR 1 1 2 EHLLMALADLDELLIR 2 2 EQAWQRPDGQPATR 2 2 ESDQGAYTCEAMNAR 2 2 FLVHDSFWALPEQFLGNK 2 2 GDLCEHEENPCQLR 1 1 2 GGSLPPHTQVHGSR 1 1 2 GMLEPVQRPDVVLVGAGYR 2 2 HPTPLALGHFHTVTLLR 1 1 2 HQTHGSQLR 1 1 2 IESSSSHVAEGETLDLNCVVPGQAHAQVTWH K22 LDGSLPPDSR 1 1 2 QPDFISFGLVGGRPEFR 1 1 2 QPQAIITWYK 1 1 2 RGGSLPSR 1 1 2 RLDLTYSFLGSQGIGQCYDSSPCER 2 2 SIVPQGGSHSLR 2 2 SPVISIDPPSSTVQQGQDASFK 2 2 VGSSLPGR 1 1 2 VISSGSVASYVTSPQGFQFR 2 2 AELLQVLQSLEAVLIQTVYNTK 1 1 CEHSQALHCHPEACGPDATCVNRPDGR 1 1 CSATGSPTPTLEWTGGPGGQLPAK 1 1 CVSTNMCIPASFHCDEESDCPDR 1 1 EDGRPVPSGTQQR 1 1 ETSLIVTIQGSGSSHVPSVSPPIR 1 1 FQGLDLNEELYLGGYPDYGAIPK 1 1 GCVGEVSVNGK 1 1 GQTVTFTCVAIGVPTPIINWR 1 1 GSMGTSGEACR 1 1 HLISTHFAPGDFQGFALVNPQR 1 1 HQGSELHFPSVQPSDAGVYICTCR 1 1 HQTHGSLLR 1 1 Supplemental Table 4 38 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total LRIPQVTPADSGEYVCHVSNGAGSR 1 1 LRLPQVSPADSGEYVCR 1 1 QVQFSEEHWVHESGRPVQR 1 1 RGGNLPAR 1 1 RGGSLPPHTQVHGSR 1 1 RPEEVCGPTQFR 1 1

SPGPNVAVNAKGSVYIGGAPDVATLTGGR 1 1 SPLPWQHR 1 1 VPGSPTNLANR 1 1 Total 103 240 22 2 367

Plasminogen activator inhibitor 1 PAI1_HUMAN 65.4 QFQADFTSLSDQEPLHVAQALQK 4 18 5 27 FIINDWVK 2 19 4 25 HNPTGTVLFMGQVMEP 4 17 4 25 EVPLSALTNILSAQLISHWK 2 15 7 24 VFQQVAQASK 3 17 4 24 KPLENLGMTDMFR 1 17 5 23 QVDFSEVER 3 15 3 21 TPFPDSSTHR 3 15 3 21 ELMGPWNKDEISTTDAIFVQR 1 16 2 19 FSLETEVDLR 3 13 1 17 SDGSTVSVPMMAQTNK 1 15 1 17 LVQGFMPHFFR 1 13 2 16 MAPEEIIMDRPFLFVVR 1 13 1 15 IEVNESGTVASSSTAVIVSAR 11 2 13 DEISTTDAIFVQR 1 8 9 GAVDQLTR 8 1 9 GMISNLLGK 9 9 LVLVNALYFNGQWK 1 7 1 9 GNMTRLPRLLVLPKFSLETEVDLR 1113 VKIEVNESGTVASSSTAVIVSAR 2 1 3 IDDKGMAPALR 1 1 LPRLLVLPKFSLETEVDLR 1 1 Total 31 250 2 48 331

Plexin domain-containing protein 2 PXDC2_HUMAN 8.9 EITVATGGFIYTGEVVHR 2 2 EIPVLVTQISSTNHPVK 1 1 VGLSDAFVVVHR 1 1 Total 4 4

Proepithelin GRN_HUMAN 34.2 EVVSAQPATFLAR 3 5 3 11

Supplemental Table 4 39 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total AVALSSSVMCPDAR 24129 VHCCPHGAFCDLVHTR 1 4 2 7 APAHLSLPDPQALKR 1 3 1 5 CITPTGTHPLAK 12115 QHCCPAGYTCNVK 2 2 4 GSEIVAGLEK 2 1 3 LPAHTVGDVK 2 1 3 SCEKEVVSAQPATFLAR 1113 CDMEVSCPDGYTCCR 2 2 CPDGSTCCELPSGK 2 2 GSEIVAGLEKMPAR 1 1 2 GTCEQGPHQVPWMEK 1 1 2 QGWACCPYR 1 1 2 APAHLSLPDPQALK 1 1 DVECGEGHFCHDNQTCCR 1 1 HCCPAGFR 1 1 SRCPDGSTCCELPSGK 1 1 WDAPLRDPALR 1 1 Total 16 32 4 13 65

Protein CYR61 CYR61_HUMAN 12.1 GLECNFGASSTALK 2 2 CAPGVGLVR 1 1 ELGFDASEVELTR 1 1 ILYNPLQGQK 1 1 Total 5 5

Protein disulfide isomerase A6 PDIA6_HUMAN 7.3 GSTAPVGGGAFPTIVER 1 1 LAAVDATVNQVLASR 1 1 Total 2 2

Protein mobA MOBA_THIFE 13.0 MIALSQEAVRSKDTINHYVLSWR 1 1

TGSGATLFVGDVGVKASSADRDASLSKLQK 1 1 Total 22

Protein NOV homolog NOV_HUMAN 36.1 TIQAEFQCSPGQIVK 6 6 LCMVRPCEQEPEQPTDKK 3 3 NNEAFLQELELK 3 3 DGQIGCVPR 2 2 KPVMVIGTCTCHTNCPK 2 2 KVEVPGECCEK 2 2 CQLDVLLPEPNCPAPR 1 1

Supplemental Table 4 40 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total FCGVCSDGR 1 1 QRGESCSDLEPCDESSGLYCDR 1 1 Total 21 21

Protein S100-A7 S10A7_HUMAN 54.5 GTNYLADVFEK 2 3 5 ENFPNFLSACDK 1 3 4 SIIGMIDMFHK 1 2 3 ENFPNFLSACDKK 1 1 KIDFSEFLSLLGDIATDYHK 1 1 Total 4 10 14

Protein S100-A8 S10A8_HUMAN 23.7 ALNSIIDVYHK 1 1 LLETECPQYIR 1 1 Total 22

Protein S100-A9 S10A9_HUMAN 44.7 NIETIINTFHQYSVK 1 1 2 KDLQNFLK 1 1 LGHPDTLNQGEFK 1 1 VIEHIMEDLDTNADK 1 1 Total 3 2 5

Protocadherin-17 PCD17_HUMAN 3.4 ASRDSSEMGAVLEQLDHPNR 1 1 EIDKLLQDCRGNDPVAVRK 1 1 Total 22

Retinoic acid receptor responder protein 2 RARR2_HUMAN 39.3 EAEEHQETQCLR 7 7 GLQVALEEFHK 6 6 LVHCPIETQVLR 5 5 HPPVQWAFQETSVESAVDTPFPAGIFVR 1 1 RGLQVALEEFHK 1 1 Total 20 20

RUN domain-containing protein 2A RUN2A_HUMAN 12.5 EITASSAVSILIKPEQETDPLPVVSR 1 1 FAPTVSDLLKESTQNVTSLLK 1 1 Total 2 2

Ryanodine receptor 3 RYR3_HUMAN 0.8 CAPEMHLIQTGKGEAIRIR 1 1 TKALSMLTEAVQCSGAHIR 1 1 Total 2 2

Serine/threonine-protein kinase SMG1 SMG1_HUMAN 1.1 KAVEHNIQIGKFSQLVMNR 1 1 LLPNMLSPDPRELQKSIEVQLLR 1 1

Supplemental Table 4 41 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total Total 2 2

Serum albumin ALBU_HUMAN 18.2 KVPQVSTPTLVEVSR 234413 CCTESLVNR 13138 YLYEIAR 12148 YICENQDSISSK 11136 CASLQKFGER 2114 VPQVSTPTLVEVSR 1 1 2 4 DVFLGMFLYEYAR 1 1 DVFLGMFLYEYARR 1 1 FKDLGEENFK 1 1 LVNEVTEFAK 1 1 RHPDYSVVLLLR 1 1 TCVADESAENCDK 1 1 Total 8 11 11 19 49

Single-stranded DNA-binding protein SSB_HUMAN 1.7 IDYGEYMDK 1 1 ISVFRPGLR 1 1 NPVTIFSLATNEMWR 1 1 QATTIIADNIIFLSDQTK 1 1 SGDSEVYQLGDVSQK 1 1 VGQDPVLR 1 1 Total 6 6

Spectrin beta chain brain 1 SPTB2_HUMAN 1.7 EAVCEVALDYK 2 2 LNDGNEYLFQAK 2 2 TAASGIPYHSEVPVSLK 1 1 Total 5 5

Stanniocalcin-2 STC2_HUMAN 30.1 VIVEMIHFK 2 10 1 13 26 GLHGICMTFLHNAGK 181717 HDLCAAAQENTR 181616 DLLLHEPYVDLVNLLLTCGEEVK 141410 AHHGEAGHHLPEPSSR 1 6 1 8 EMVSQLQR 13138 SFIKDALK 1 1 Total 7 39 6 34 86

Striated muscle preferentially expressed protein kinase SPEG_HUMAN 1.0 LSALGRSPR 1 1 SRAIQECRSPVPPPAADPPEARTK 1 1 Total 2 2

Supplemental Table 4 42 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total

Sulfhydryl oxidase 1 QSOX1_HUMAN 50.3 NNEEYLALIFEK 4 1 5 DFNIPGFPTVR 4 4 EVALDLSQHK 4 4 LAGAPSEDPQFPK 4 4 FGVTDFPSCYLLFR 3 3 IYMADLESALHYILR 2 1 3 LDVPVWDVEATLNFLK 2 1 3 LEEIDGFFAR 3 3 NGSGAVFPVAGADVQTLR 3 3 SALYSPSDPLTLLQADTVR 3 3 VLNTEANVVR 3 3 AAPGQEPPEHMAELQR 2 2 AHFSPSNIILDFPAAGSAAR 2 2 AWRPALYLAALDCAEETNSAVCR 2 2 DCASHFEQMAAASMHR 2 2 FPVLEGQR 2 2 IEVGRFPVLEGQR 2 2 KVNWIGCQGSEPHFR 2 2 SFYTAYLQR 2 2 VNWIGCQGSEPHFR 2 2 VPVLMESR 2 2 CNSGSGPPPSLLLLLLWLLAVPGANAAPR 1 1 DVQNVAAAPELAMGALELESR 1 1 ELCSACHNER 1 1 GFPCSLWVLFHFLTVQAAR 1 1 GYVHYFFGCR 1 1 KFGVTDFPSCYLLFR 1 1 RVLNTEANVVR 1 1 VGSPNAAVLWLWSSHNR 1 1 Total 62 4 66

Tenascin TENA_HUMAN 3.1 SQTVSAIATTAMGSPK 2 2 ASVTGYLLVYESVDGTVK 1 1 ITYVPITGGTPSMVTVDGTK 1 1 KQSEPLEITLLAPER 1 1 Total 5 5

TGF beta-2 TGFB2_HUMAN 30.9 IEQLSNMIVK 1 5 6 FAGIDGTSTYTSGDQK 1 4 5 ALDAAYCFR 1 2 3 TPHLLLMLLPSYR 3 3

Supplemental Table 4 43 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total AEGEWLSFDVTDAVHEWLHHK 2 2 FDVSAMEK 2 2 ERSDEEYYAK 1 1 GYNANFCAGACPYLWSSDTQHSR 1 1 IELYQILK 1 1 SKDLTSPTQR 1 1 Total 3 22 25

Thrombospondin-1 TSP1_HUMAN 33.2 FVFGTTPEDILR 9 1 10 LCNNPTPQFGGK 8 8 IPESGGDNSVFDIFELTGAAR 5 1 6 TIVTTLQDSIR 6 6 DCVGDVTENQICNK 5 5 GGVNDNFQGVLQNVR 5 5 IEDANLIPPVPDDKFQDLVDAVR 4 4 MENAELDVPIQSVFTR 3 1 4 NALWHTGNTPGQVR 4 4 QHVVSVEEALLATGQWK 4 4 QVTQSYWDTNPTR 4 4 AGTLDLSLTVQGK 3 3 CENTDPGYNCLPCPPR 3 3 DLQAICGISCDELSSMVLELR 2 1 3 FTGSQPFGQGVEHATANK 3 3 SITLFVQEDR 3 3 CNYLGHYSDPMYR 2 2 DDDYAGFVFGYQSSSR 2 2 DNCQYVYNVDQR 2 2 KVTEENKELANELR 2 2 NNEEWTVDSCTECHCQNSVTICK 2 2 AQLYIDCEK 1 1 FYVVMWK 1 1 GFLLLASLR 1 1 GTSQNDPNWVVR 1 1 IMADSGPIYDK 1 1 KDHSGQVFSVVSNGK 1 1 KIMADSGPIYDK 1 1 Total 88 4 92

Titin TITIN_HUMAN 0.9 ATLTWTPPLEDGGSPIKSYILEK 1 1 EAPPKARVPEEPKRAVPEEK 1 1 EISIEAMDFVDRLLVKERKSR 1 1 ESEFVRFSKTENKITLSIK 1 1

Supplemental Table 4 44 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total FESVSADQMTLSWFPPKDDGGSK 1 1 IYAMNSEGLGEPALVPGTPKAEDR 1 1 KPEAPPAKVPEVPKEVVPEKKVAVPK 1 1 LKIGLITKNTVHLSWKPPK 1 1 NAAGVFSEPSESTGAITARDEVDPPR 1 1 NNVATLVFNQVDINDSGEYICK 1 1 QTGECKLVISMTFADDAGEYTIVVR 1 1 RDDKTLESGDKYDVIADGK 1 1 SKEEHER 1 1 TEEGYYEAITAVELK 1 1 VKVMDVPGPPKDLKVSDITRGSCR 1 1 VPEVPKEVVPEKKVAVPKKPEVPPAK 1 1 Total 3 11 2 16

Trinucleotide repeat-containing gene 6A protein TNR6A_HUMAN 2.3 NSGSSSSLNTTLPSTSAWSSIR 1 1 TDNGTEAWGSSATQTFNSGACIDK 1 1 Total 2 2

Tyrosine-protein kinase BTK BTK_HUMAN 3.9 YPVSQQNK 1 1 YPVSQQNKNAPSTAGLGYGSWEIDPK 1 1 Total 2 2

Uncharacterized protein C6orf174 CF174_HUMAN 4.3 EGGAPQPPPPRGWR 1 1 TGSRTGPPASIRGGGGGAEKATPLAPK 1 1 Total 2 2

Vacuolar protein sorting-associated protein VP13D_HUMAN 1.5 AQSPVSGPNVAHLTDGATLNDR 1 1 LLAESLPR 1 1 SLPSHMEEAPNVFQLYQRPTSASRK 1 1 YFNKLKGGLLSTDDKEEMCR 1 1 Total 2 2 4

Vacuolar protein sorting-associated protein 13A VP13A_HUMAN 1.5 LFFILLTPVNHSTADKIPLTKVGRR 1 1 VPEYKTVISFHSKDQLNITLSK 1 1 Total 2 2

Vimentin VIME_HUMAN 31.5 DGQVINETSQHHDDLE 1 1 EEAENTLQSFR 1 1 EMEENFAVEAANYQDTIGR 1 1 ETNLDSLPLVDTHSK 1 1

Supplemental Table 4 45 Defining the elastic fiber interactome

SwissProt Entry Total % ARPE ARPE HDF HDF Protein Identity Name Coverage Peptide sequence Matrix Media Matrix Media Total FADLSEAANR 1 1 ILLAELEQLK 1 1 ILLAELEQLKGQGK 1 1 KVESLQEEIAFLK 1 1 LLEGEESR 1 1 LQEEMLQREEAENTLQSFR 1 1 NLQEAEEWYK 1 1 SLYASSPGGVYATR 1 1 VELQELNDR 1 1 Total 2 11 13

Zinc transporter ZIP10 S39AA_HUMAN 2.9 GHQDLDPDNEGELR 1 1 LLTNLGLGER 1 1 Total 22

Zinc-alpha-2-glycoprotein ZA2G_HUMAN 1.5 AGEVQEPELR 1 1 AREDIFMETLK 1 1 EIPAWVPFDPAAQITK 1 1 HVEDVPAFQALGSLNDLQFFR 1 1 NILDRQDPPSVVVTSHQAPGEK 1 1 QDPPSVVVTSHQAPGEK 1 1 QKWEAEPVYVQR 1 1 YSLTYIYTGLSK 1 1 YYYDGKDYIEFNK 1 1 Total 9 9

Unique Peptide Sequence Total (Number of 970 1981 765 1130 6032 Unique Peptide Sequences) (470) (683) (374) (500) (1211)

Supplemental Table 4 46 Defining the elastic fiber interactome

SUPPLEMENTAL TABLE 5 Peptide sequences identified for each protein for each bait protein

Scaffold was used to validate protein and peptide identification. Only proteins with >2 unique peptides sequences were counted for each affinity experiment, and each peptide had greater than 95.0% probability as specified by the Peptide Prophet algorithm (38). Sequences of each peptide are shown, the total number of unique peptides and the bait protein used which the identified peptide was detected . Collagen α-1 (VIII) and α-2 (VIII) results are shown in Part 2 (Below). Part 1 Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin Actin cytoplasmic 1 ACTB_HUMAN DSYVGDEAQSK 21 IIAPPER 11 AGFAGDDAPR 1 GYSFTTTAER 1 VAPEEHPVLLTEAPLNPK 1 Total 53

Activated RNA polymerase II TCP4_HUMAN EQISDIDDAVR 1 EYWMDPEGEMKPGR 1 Total 2

Agrin AGRIN_HUMAN LELGIGPGAATR 1111 EPLYVGGAPDFSK 111 VLGAPVPAFEGR 111 AAAVSSGFDGAIQLVSLGGR 11 AIVDVHFDPTTAFR 11 ALEPQGLLLYNGNAR 11 EAAYVCLCPGGFSGPHCEK 11 GKDFLALALLDGR 11 MALEVVFLAR 11 QVDVTSFAGHPCTR 11 SFLAFPTLR 11 SIESTLDDLFR 11 TPSLDAEGSNCPATK 1 1 VLGESPVPHTVLNLK 11 VLPEGGAQCECPLGR 11 DFLALALLDGR 1 EAACLQQTQIEEAR 1 FGALCEAETGR 1 QLLTPEHVLR 1 SPCQPNPCHGAAPCR 1 SREPVTLGAWTR 1 TDGKGDFVSLALR 1 Total 24 4 1514 2

A-kinase anchor protein 9 AKAP9_HUMAN ALEIDVEEQVSR 1 LQMEAQRICLSLVYSTHVDQVR 1 Total 2

Alpha-2-macroglobulin A2MG_HUMAN GCVLLSYLNETVTVSASLESVRGNR 2 QTVSWAVTPK 2 Total 4

Amine oxidase AOFA_HUMAN TYTIRNEHVDYVDVGGAYVGPTQNR 1 WSGYMEGAVEAGER 1 Total 2

Amyloid A4_HUMAN CLVGEFVSDALLVPDK 1 MDVCETHLHWHTVAK 1 STNLHDYGMLLPCGIDK 1

Supplemental Table 5 47 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin Total 3

Annexin A2 ANXA2_HUMAN TNQELQEINR 21 GLGTDEDSLIEIICSR 1 1 GVDEVTIVNILTNR 1 1 QDIAFAYQR 11 RAEDGSVIDYELIDQDAR 1 1 SLYYYIQQDTK 2 TDLEKDIISDTSGDFR 1 1 TPAQYDASELK 2 AYTNFDAERDALNIETAIK 1 DALNIETAIK 1 LSLEGDHSTPPSAYGSVK 1 SALSGHLETVILGLLK 1 SYSPYDMLESIR 1 SYSPYDMLESIRK 1 TDLEKDIISDTSGDFRK 1 Total 5 17 2

Annexin V ANXA5_HUMAN GLGTDEESILTLLTSR 2 GTVTDFPGFDER 2 VLTEIIASR 2 DLLDDLKSELTGK 1 GAGTDDHTLIR 1 SEIDLFNIR 1 Total 9

Apolipoprotein D APOD_HUMAN NILTSNNIDVK 1 NPNLPPETVDSLK 1 VLNQELR 1 Total 3

ATP-binding cassette sub-family A member 13 ABCAD_HUMAN AETYFFSSGGDNLDLTRVLLRKFR 1 EIQDLAEEIHGMMDKAKNLK 1 NPVLFLAEFFWPCILFVILTVLR 1 Total 3

BigH3 BGH3_HUMAN DLLNNHILK 221 13 1 ILGDPEALR 4211 1 1 SPYQLVLQHSR 32 112 1 LTLLAPLNSVFK 321 12 STVISYECCPGYEK 41 13 QAGLGNHLSGSER 33 2 DGTPPIDAHTR 31 2 1 FSMLVAAIQSAGLTETLNR 21 13 QHGPNVCAVQK 32 1 1 SLQGDKLEVSLK 21 12 1 GDELADSALEIFK 33 IPSETLNR 41 1 VLTPPMGTVMDVLK 21 11 1 EGVYTVFAPTNEAFR 31 1 YLYHGQTLETLGGK 11 12 YHIGDEILVSGGIGALVR 12 DILATNGVIHYIDELLIPDSAK 2 NSLCIENSCIAAHDK 11 TLFELAAESDVSTAIDLFR 11 VISTITNNIQQIIEIEDTFETLR 11 YGTLFTMDR 11 Supplemental Table 5 48 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin GCPAALPLSNLYETLGVVGSTTTQLYTDR 1 NSLCIENSCIAAHDKR 1 Total 42 24 4 2 10 35 10

Bile salt export pump ABCBB_HUMAN AYAKAGVVADEVISSMRTVAAFGGEK 1 DKQALEMVGQITNEALSNIR 1 Total 2

Bullous pemphigoid antigen 1 isoforms 1/2/3/4/5/8 BPA1_HUMAN EVRNLNAELNASK 1 IQADGFKDQLRSTNEHLHK 1 QMEKDLAFQKQVAEK 1 Total 3

Bullous pemphigoid antigen 1 isoforms 6/9/10 BPAEA_HUMAN EKKEAVTSCQEQLDAFQVLVKSLK 1 FEEVLAWAKQHQQRLASALAGLIAK 1 Total 2

Cadherin-related tumor suppressor homolog FAT1_HUMAN AEISEFAPPNTPVVMVKAIPAYSHLR 1 LYEMEILAADRGMKLYGSSGISSMAK 1 Total 2

Calsyntenin-1 CSTN1_HUMAN AASEFESSEGVFLFPELR 221 EGLDLQVLEDSGR 211 ISLSGVHHFAR 211 LTVTAYDCGK 22 HHYSLYVHGCR 21 IPDGVVSVSPK 12 AMQHISYLNSR 11 ATVHIQVNDVNEYAPVFK 11 IHGQNVPFDAVVVDK 11 KYRPAEFHWK 2 VIDCLYTCK 2 DYSFTIQAYDCGK 1 ETILCSSDK 1 FAESFEVTVTK 1 GNLAGLTLR 1 GVQIQAHPSQLVLTLEGEDLGELDK 1 ISIKPTCTPGWQGWNNR 1 QFPTPGIR 1 QYDSILR 1 Total 21 10 8 2

Calsyntenin-2 CSTN2_HUMAN EGLDINSLESLGQGIK 21 1 VISCLQACK 21 1 SPIDCELQK 21 DAPVPFAGEICAFK 11 DVNEFAPTFK 2 ETILCNSDK 2 QECLELNHSELHQR 11 QFPTAGVR 2 AVVHIQVK 1 ETILCNSDKTEMNR 1 EYTFIIQAYDCGAGPHETAWK 1 GTDHFWRPAAQFESAR 1 GVTLFPDIK 1 LCWVPLLLALGVGSGSGGGGDSRQR 1 Supplemental Table 5 49 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin YEQVLHHIR 1 YHFNPSQSILVMEGDDIGNINR 1 Total 16 6 7

Cathepsin H CATH_HUMAN MALNQFSDMSFAEIK 1 VNHAVLAVGYGEK 1 Total 2

Chromodomain-helicase-DNA- binding protein 5 CHD5_HUMAN AEEKEPIETQQNGDKEEDDEGK 1 EPIETQQNGDKEEDDEGKKEDKKGK 1 Total 2

Coiled-coil domain-containing protein CCD80_HUMAN IDHFQLDNEKPMR 1 SVFDLIDTFQSR 1 Total 2

Collagen alpha-1(I) chain CO1A1_HUMAN SLSQQIENIR 11 1 11 VLCDDVICDETK 11 1 1 DGEAGAQGPPGPAGPAGER 111 GSPGEAGRPGEAGLPGAK 111 GVQGPPGPAGPR 111 ALLLQGSNEIEIR 11 DRDLEVDTTLK 11 FTYSVTVDGCTSHTGAWGK 11 GPPGPMGPPGLAGPPGESGR 11 EGAPGAEGSPGR 1 GANGAPGIAGAPGFPGAR 1 GEPGSPGENGAPGQMGPR 1 GETGPAGPAGPVGPVGAR 1 GETGPAGRPGEVGPPGPPGPAGEK 1

GLTGPIGPPGPAGAPGDKGESGPSGPAGPTGAR 1 GLTGSPGSPGPDGK 1 GPAGPQGPR 1 GSAGPPGATGFPGAAGR 1 NSVAYMDQQTGNLK 1 STGGISVPGPMGPSGPR 1 TGPPGPAGQDGRPGPPGPPGAR 1 VGPPGPSGNAGPPGPPGPAGK 1 Total 85 31382

Collagen alpha-1(IV) chain CO4A1_HUMAN SAPFIECHGR 14 AHGQDLGTAGSCLR 13 HSQTIDDPQCPSGTK 13 ILYHGYSLLYVQGNER 3 FSTMPFLFCNINNVCNFASR 2 GTCNYYANAYSFWLATIER 2 KFSTMPFLFCNINNVCNFASR 1 NDYSYWLSTPEPMPMSMAPITGENIRPFISR 1 Total 319

Collagen alpha-1(VIII) chain CO8A1_HUMAN GFLDQASGSAVLLLRPGDR 1 GGNVWVALFK 1 Total 2

Collagen alpha-1(XI) chain COBA1_HUMAN AAYDYCEHYSPDCDSSAPK 1 ALDFHNSPEGISK 1 Supplemental Table 5 50 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin ILDEEVFEGDIQQFLITGDPK 1 Total 3

Collagen alpha-1(XII) chain COCA1_HUMAN GEVQTVTFDTEEVK 11 TEFNLNQYYQR 11 ALALGALQNIR 1 GDTTNTVLQGLK 1 GPGDLEAPSNLVISER 1 HVFIVDDFESFEK 1 ITVIAVYEDGDGGHLTGNGR 1 IYIDCYEIIEK 1 NNVILQPLQPDTPYK 1 SLLQAVANLPYK 1 TYQIGWDTFCVK 1 VEDIIEAINTFPYR 1 VSWTPPSDSVDR 1 VYDPSTSTLNVR 1 YKVEYYPVSGGK 1 Total 98

Collagen alpha-1(XVIII) chain COIA1_HUMAN TEAPSATGQASSLLGGR 1133 11 2 LQDLYSIVR 1113 1 1 GADFQCFQQAR 1122 1 AVGLAGTFR 13 1 DELLFPSWEALFSGSEGPLKPGAR 23 EETGAALKPR 12 11 LTESYCETWR 111 1 GTDNEVAALQPPVVQLHDSNPYPR 2 1 SVWHGSDPNGR 21 IFSFDGKDVLR 11 LSGVQDGHQDISLLYTEPGAGQTHTAASFR 1 1 AAVPIVNLK 1 DFQPVLHLVALNSPLSGGMR 1 ELLREETGAALKPR 1 GERGEPGGGGFFGSSLPGPPGPPGPPGPR 1 Total 5 3 14 20 4 3 2 9

Collagen alpha-2(I) chain CO1A2_HUMAN TGEVGAVGPPGFAGEK 1 111 GETGPSGPVGPAGAVGPR 111 TGPPGPSGISGPPGPPGPAGK 111 FTYTVLVDGCSK 11 SLNNQIETLLTPEGSR 11 AVILQGSNDVELVAEGNSR 1

GAPGAVGAPGPAGATGDRGEAGAAGPAGPAGPR 1 GDGGPPGMTGFPGAAGR 1 GEIGAVGNAGPAGPAGPR 1 GEPGSAGPQGPPGPSGEEGK 1 GEQGPAGPPGFQGLPGPSGPAGEVGKPGER 1 GESGNKGEPGSAGPQGPPGPSGEEGK 1 GLHGEFGLPGPAGPR 1 GLPGVAGAVGEPGPLGIAGPPGAR 1 GPPGAVGSPGVNGAPGEAGR 1 GPPGESGAAGPTGPIGSR 1 HGNRGETGPSGPVGPAGAVGPR 1 VYCDFSTGETCIR 1 Total 5 6 11 5

Collagen alpha-2(IV) chain CO4A2_HUMAN AHNQDLGLAGSCLAR 3 Supplemental Table 5 51 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin ATPFIECNGGR 12 SVSIGYLLVK 3 CSVCEAPAIAIAVHSQDVSIPHCPAGWR 2 FSTMPFLYCNPGDVCYYASR 2 HSQTDQEPMCPVGMNK 2 LWSGYSLLYFEGQEK 2 SYWLSTTAPLPMMPVAEDEIKPYISR 2 GDPGQHGLPGFPGLKGVPGNIGAPGPKGAK 1 GFPGSPGSDIHGDPGFPGPPGER 1 GPRGEQGFMGNTGPTGAVGDRGPK 1

SLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFR 1 YSFWLTTIPEQSFQGSPSADTLK 1 Total 222

Collagen alpha-3(IV) chain CO4A3_HUMAN ASPFLECHGR 1 CIEGPRGAQGLPGLNGLKGQQGR 1 GFTGPEGLPGPQGPKGFPGLPGLTGSKGVR 1 Total 3

Collagen alpha-3(V) chain CO5A3_HUMAN GEKGEDGFPGFKGDVGLKGDQGKPGAPGPR 1 TGEVGPLGERGPPGPPGPPGEQGLPGLEGR 1 Total 2

Complement C1q C1QT3_HUMAN FGAPVSGVYFFTFSMMK 1 FSTFAGFLLFETK 1 Total 2

Complement C3 CO3_HUMAN ACEPGVDYVYK 1 TIYTPGSTVLYR 1 Total 2

Complement factor D CFAD_HUMAN AVPHPDSQPDTIDHDLLLLQLSEK 1 VDRDVAPGTLCDVAGWGIVNHAGR 1 Total 2

Dedicator of cytokinesis protein 2 DOCK2_HUMAN EDGTTLHDGFHDLVVLKGDSK 1 GEQCEPILRTLKALEYVFKFIVR 1 Total 2

Dickkopf-related protein 1 DKK1_HUMAN GQEGSVCLR 1 SSDCASGLCCAR 1 Total 2

Elastin ELN_HUMAN AGYPTGTGVGPQAAAAAAAK 1 LPGGYGLPYTTGK 1 Total 2

Extracellular matrix protein 1 ECM1_HUMAN AWEDTLDKYCDR 11 ELLALIQLER 11 FSCFQEEAPQPHYQLR 11 LDGFPPGRPSPDNLNQICLPNR 11 QGETLNFLEIGYSR 11 VTPNLMGHLCGNQR 11 ACPSHQPDISSGLELPFPPGVPTLDNIK 1 ELPSLQHPNEQK 1 FCEAEFSVK 1 LTFINDLCGPR 1 Supplemental Table 5 52 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin LVWEEAMSR 1 Total 710

Fibrillin-1 FBN1_HUMAN YEDEECTLPIAGR 2 3 5 3212236125 NTPEYEELCPR 2 1 5 2112137154 CSNQLPQSITK 1211611 9 6 CWSPGVTVAPEMCPIR 14 1 1 10 4 TLPGGNQCIVPICR 1 31611 21 5 CECEIGYELDR 1 31112 114 CLCYDGFMASEDMK 2 2 112410 GYIGTHCGQPVCESGCLNGGR 116113 CPPGFYTSPDGTR 1 13 111 4 GQCVNTPGDFECK 3 3 11 4 GTCWQTVIDGR 3 2 1 1112541 ISPDLCGR 1 3 1484 LETCFLR 1 4 2 482 YNAYCCPGWK 1115 2 2 CSCSPGWIGDGIK 1311113 CACTYGFTGPQCER 14 1 1 3 TGCTDINECEIGAHNCGK 11212 12 CDSGFALDSEER 6102 GDNGDTACSNEIGVGVSK 25 12 8 MIPSLCTHGK 11 583 CECDMGFVPSADGK 13 1210 YQCACNPGYHSTPDR 1 2 374 AWGHPCEMCPAQPHPCR 11 1 1 3 CECFPGLAVGLDGR 1294 GGGGHDALKGPNVCGSR 15 1 CDEGYESGFMMMK 1 12263 CQCPSGMTLDATGR 1 11255 GFMTNGADIDECK 1 1 2 461 SGNCYLDIRPR 213 CICNSGYEVDSTGK 1 1 1 1541 TCVDINECLLEPR 112 6121 CIDVRPGYCYTALTNGR 12 1 GFIYKPDLK 21 55 GQCIKPLFGAVTK 11 18 2 CLCPEGFSLSSSGR 1 1 5122 EITNGKPFFK 2 442 NCMDIDECQR 1 254 NCMDIDECQRDPLLCR 1 1 253 RGFIPNIR 912 TCVDVNECDLNPNICLSGTCENTK 39 TGPCFTVISNQMCQGQLSGIVCTK 81 12 ACEDIDECSLPNICVFGTCHNLPGLFR 29 CICPPGYSLQNEK 2 5112 GWGPHCEICPFQGTVAFK 1 2 152 MDACCCSVGAAWGTEECEECPMR 452 SIQHCNIR 10 TNDDQTMCLDINECER 1612 CECPVGFFYNDK 1512 CICKPGFQLASDGR 117 GGVCHNTEGSYR 252 GTQCEDIDECEVFPGVCK 1251 AGYQSTLTR 1 1 231 NCEDMDECSIR 1151 SCTDIDECEDNPNICDGGQCTNIPGEYR 143 VCVDTHMR 1421 CEININGATLK 34 Supplemental Table 5 53 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin CPTGYYLNEDTR 13111 DINECETPGICMNGR 16 GEGWGDPCELCPTEPDEAFR 331 GSFICHCDMGYSGK 7 GYILQEDGR 115 IGQGHCVSGMGMGR 12 31 AWGTPCEMCPAVNTSEYK 6 CIPTPGSYR 411 EGYCFTEVLQNMCQIGSSNR 141 HGQCINTDGSYR 24 LLVCEDIDECQNGPVCQR 15 CINTFGSFQCR 5 CQHGCQNIIGGYR 23 EIPGVCENGVCINMVGSFR 311 GICQNTPGSFTCECQR 23 NCVDINECVLNSLLCDNGQCR 41 NTPGSFVCTCPK 5 CPPGFTQHHTSCIDNNECTSDINLCGSK 22 CPVGYVLR 4 DIDECLQNGR 4 GFSLDQTGSSCEDVDECEGNHR 13 GSYHCICK 13 HAVCTNTAGSFK 4 HSCGDGFCSRPNMCTCPSGQIAPSCGSR 4 IKGTQCEDIDECEVFPGVCK 4 KLCPHGR 4 NSPGSFICECSSESTLDPTK 4 QHNCQFLCVNTIGGFTCK 13 RGGGGHDALKGPNVCGSR 4 RPDGEGCVDENECQTKPGICENGR 31 SQCCSSLGAAWGSPCTLCQVDPICGK 121 CTDLDECSNGTHMCSQHADCK 3 CVNTDGSYR 12 CVNTYGSYECK 3 KCAPGTCQNLDGSYR 3 MCKDEDECEEGKHDCTEK 3 NGLCVNTR 3 NLIGTYMCICGPGYQR 111 NMCLNGMCINEDGSFK 3 NTEGSYQCSCPK 12 QECCCALK 3 RGQCIKPLFGAVTK 3 TGYTPDITGTSCVDLNECNQAPKPCNFICK 21 VLPVNVTDYCQLVR 12 ASCCCSLGK 2 CAPGTCQNLDGSYR 2 CEDIDECSTIPGICEGGECTNTVSSYFCK 2 CINTDGSFHCVCNAGFHVTR 2 CRCDSGFALDSEER 11 DEDECEEGKHDCTEK 2 DGKNCEDMDECSIR 2 EGYCFTEVLQNMCQIGSSNRNPVTK 2 GFMTNGADIDECKVIHDVCR 2 GFQLDLR 2 GKTGCTDINECEIGAHNCGK 2 GNPEPPVSGEMDDNSLSPEACYECK 2 GSFKCQCPSGMTLDATGR 2 GWGPHCEICPFQGTVAFKK 2 ICLDIR 11 Supplemental Table 5 54 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin ILELLPALTTLTNHNR 2 NCTDIDECR 11 QICPYGSGIIVGPDDSAVDMDECK 2 QICPYGSGIIVGPDDSAVDMDECKEPDVCK 11 SCKDLDECATK 2 TCEDIDECESSPCINGVCK 2 YLIESGNEDGFFK 2 CINTFGSFQCRCPTGYYLNEDTR 1 CRCDSGFALDSEERNCTDIDECR 1 CRNTIGSFK 1 CWSPGVTVAPEMCPIRATEDFNK 1 DPLLCR 1 EGYTGDGFTCTDLDECSENLNLCGNGQCLNAPGGY R 1 FTSTGQCNDR 1 GECIDVDECEK 1 GSFICHCDMGYSGKK 1 GSYTCECNDGFTASPNQDECLDNR 1 GTQCEDIDECEVFPGVCKNGLCVNTR 1

ILCPGGEGFRPNPITVILEDIDECQELPGLCQGGK 1 KKPVAGTYSLQISSTPLYK 1 KMCCCSYNIGR 1 MCCCSYNIGR 1 MQCCCDAGR 1 MSYCYAK 1 NAECINTAGSYR 1 NGQCINTVGSFQCQCNEGYEVAPDGR 1 NTIGSFNCR 1 SECCCDGGRGWGPHCEICPFQGTVAFK 1 VGCVDTR 1 VIHDVCRNGECVNDR 1 Total 5 27 34 32 230 77 60 96 139 114 159 216

Fibrillin-2 FBN2_HUMAN CLCNEGYELTPDGK 1 LSSTGLICIDSLK 1 Total 2

Fibrillin-3 FBN3_HUMAN CQHGCQNQLGGYR 1 GYLLEEDGR 1 Total 2

Fibronectin FINC_HUMAN SYTITGLQPGTDYK 3 112 SSPVVIDASTAIDAPSNLR 1 1 121 ISCTIANR 131 VPGTSTSATLTGLTR 11 1 2 DLQFVEVTDVK 121 EINLAPDSSSVVVSGLMVATK 112 GEWTCIAYSQLR 112 IYLYTLNDNAR 211 NLQPASEYTVSLVAIK 1 111 WLPSSSPVTGYR 211 FLATTPNSLLVSWQPPR 111 NTFAEVTGLSPGVTYYFK 111 VDVIPVNLPGEHGQR 21 DSMIWDCTCIGAGR 11 GATYNIIVEALK 2 HTSVQTTSSGSGPFTDVR 11 ITYGETGGNSPVQEFTVPGSK 11 Supplemental Table 5 55 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin QYNVGPSVSK 2 RPGGEPSPEGTTGQSYNQYSQR 11 TFYSCTTEGR 2 TYLGNALVCTCYGGSR 11 VGDTYERPK 11 VTIMWTPPESAVTGYR 11 WSRPQAPITGYR 11 DDKESVPISDTIIPAVPPPTDLR 1 EATIPGHLNSYTIK 1 EESPLLIGQQSTVSDVPR 1 ESKPLTAQQTTK 1 EVTSDSGSIVVSGLTPGVEYVYTIQVLR 1 FTNIGPDTMR 1 FTQVTPTSLSAQWTPPNVQLTGYR 1 GATYNIIVEALKDQQR 1 GDSPASSKPISINYR 1 GFNCESKPEAEETCFDK 1 GLAFTDVDVDSIK 1 GLKPGVVYEGQLISIQQYGHQEVTR 1 IAWESPQGQVSR 1 IGDQWDKQHDMGHMMR 1 LLCQCLGFGSGHFR 1 SDTVPSPR 1 TEIDKPSQMQVTDVQDNSISVK 1 TETITGFQVDAVPANGQTPIQR 1 TGLDSPTGIDFSDITANSFTVHWIAPR 1 TIKPDVR 1 TKTETITGFQVDAVPANGQTPIQR 1 VTDATETTITISWR 1 VTWAPPPSIDLTNFLVR 1 YEKPGSPPR 1 YEVSVYALK 1 Total 6 3 2 272 231921

Fibulin-1 FBLN1_HUMAN DCSLPYATESK 11 TGYYFDGISR 11 CCHCCLLGR 1 GYHLNEEGTR 1 SQETGDLDVGGLQETDK 1 Total 25

Fibulin-3 FBLN3_HUMAN EHIVDLEMLTVSSIGTFR 1 NPCQDPYILTPENR 1 Total 2

Follistatin-related protein FSTL1_HUMAN CALEDETYADGAETEVDCNR 11 ICANVFCGAGR 11 CVCACGNWVCTAMTCDGK 1 ECAVTEKGEPTCLCIEQCKPHK 1 GLCVDALIELSDENADWK 1 LSFQEFLK 1 Total 53

Golgin subfamily B member 1 GOGB1_HUMAN ILALEKEKEQLQKKLQEALTSRK 1 REKVHLEETIGEIQVTLNK 1 Total 2

Hemoglobin subunit alpha HBA_HUMAN TYFPHFDLSHGSAQVK 1 VGAHAGEYGAEALER 1 Supplemental Table 5 56 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin Total 2

Heterogeneous nuclear ribonucleoprotein M HNRPM_HUMAN FGSGMNMGRINEILSNALKR 1 GIGMGNIGPAGMGMEGIGFGINK 1 Total 2

High mobility group protein HMGA1_HUMAN EPSEVPTPK 1 KQPPVSPGTALVGSQK 1 Total 2

Histone H2A type 2-A AN,H2A2C_HUMAN AGLQFPVGR 2312 1 HLQLAIR 21 2 1 VTIAQGGVLPNIQAVLLPK 11 ARAKAKSRSSRAGLQFPVGR 1 LLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAG NAARDNK 1 Total 4624 3

Histone H2B H2B1M_HUMAN LLLPGELAK 11 12 2 1 QVHPDTGISSK 122 1 AMGIMNSFVNDIFER 22 ESYSVYVYK 1 KESYSVYVYK 1 MPEPVKSAPVPKKGSKKAINK 1 Total 42 27 4 2

Histone H3 H33_HUMAN YRPGTVALR 11 1 KLPFQR 11 STELLIR 1 Total 22 2

Histone H4. H4_HUMAN ISGLIYEETR 442104 82 DNIQGITKPAIR 4 2 824 8 VFLENVIR 2 2 2624 4 DAVTYTEHAK 228 2 TVTAMDVVYALK 22 24 4 KTVTAMDVVYALK 4 2 TVTAMDVVYALKR 2 2 Total 18 12 8 36 4 12 28 4

Insulin-like growth factor II IGF2_HUMAN GIVEECCFR 1 SCDLALLETYCATPAK 1 Total 2

Insulin-like growth factor-binding protein 3 IBP3_HUMAN ALAQCAPPPAVCAELVR 2131 YGQPLPGYTTK 21121 FLNVLSPR 111 EMEDTLNHLK 11 GVHIPNCDKK 11 SAGSVESPSVSSTHR 11 GVHIPNCDK 1 Total 43476

Insulin-like growth factor-binding protein 4 IBP4_HUMAN GELDCHQLADSFRE 211 12 1 CRPPVGCEELVR 22 12 THEDLYIIPIPNCDR 1121 Supplemental Table 5 57 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin EDARPVPQGSCQSELHR 21 1 GELDCHQLADSFR 1 QCHPALDGQR 1 TGVKLPGGLEPKGELDCHQLADSFR 1 Total 662 36 4

Insulin-like growth factor-binding protein 5 IBP5_HUMAN ALSMCPPSPLGCELVK 11 QDEEKPLHALLHGR 11 AVYLPNCDR 1 HMEASLQELK 1 Total 22 2

Insulin-like growth factor-binding protein 7 IBP7_HUMAN AGAAAGGPGVSGVCVCK 111122 ITVVDALHEIPVK 1 1 1 1112 TELLPGDRDNLAIQTR 11 2 11 GTCEQGPSIVTPPK 212 EDAGEYECHASNSQGQASASAK 11 1 HEVTGWVLVSPLSK 111 GEGEPCGGGGAGR 1 GGPEKHEVTGWVLVSPLSK 1 GYCAPGMECVK 1 ITVVDALHEIPVKK 1 SRYPVCGSDGTTYPSGCQLR 1 Total 3 4 9 3469

Keratin type I cytoskeletal K1C16_HUMAN ALEEANADLEVK 2 ASLENSLEETK 2 GQTGGDVNVEMDAAPGVDLSR 2 IIAATIENAQPILQIDNAR 2 TRLEQEIATYR 2 APSTYGGGLSVSSR 1 TEELNKEVASNSELVQSSR 1 TKYEHELALR 1 VLDELTLAR 1 VLQGLEIELQSQLSMK 1 Total 15

Keratin type I cytoskeletal 10 K1C10_HUMAN QSLEASLAETEGR 4 2 33616212 ALEESNYELEGK 6 2 111515312 1 GSLGGGFSSGGFSGGSFSR 5 13 173 1 1 LKYENEVALR 4 3 113142 1 1 LENEIQTYR 4 1 124 4111 IRLENEIQTYR 1 1 32 14211 1 NVQALEIELQSQLALK 31 17211 VLDELTLTK 3 114 2121 SQYEQLAEQNR 3 115 111 VTMQNLNDR 4 24111 AETECQNTEYQQLLDIK 11316 SLLEGEGSSGGGGR 514 11 QSVEADINGLR 3 1 1211 11 DAEAWFNEK 2 1 1 2111 ELTTEIDNNIEQISSYK 12 141 SQYEQLAEQNRK 2 2 1 112 GSSGGGCFGGSSGGYGGLGGFGGGSFR 12 4 LASYLDKVR 13 1 1 1 NQILNLTTDNANILLQIDNAR 1 1 121 ADLEMQIESLTEELAYLK 221 Supplemental Table 5 58 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin SKELTTEIDNNIEQISSYK 131 ADLEMQIESLTEELAYLKK 11 1 HYSSSRSGGGGGGGGCGGGGGVSSLR 11 LAADDFR 1 1 LASYLDK 11 NVSTGDVNVEMNAAPGVDLTQLLNNMR 11 YENEVALR 1 1 HGNSHQGEPR 1 IKEWYEK 1 ISSSKGSLGGGFSSGGFSGGSFSR 1 NHEEEMKDLR 1 QSVEADINGLRR 1 SGGGGGGGGCGGGGGVSSLR 1 TIDDLKNQILNLTTDNANILLQIDNAR 1 YCVQLSQIQAQISALEEQLQQIR 1 Total 2 47 19 2 28 25 39 12 66 25 14 25 4

Keratin type I cytoskeletal 14 K1C14_HUMAN VLDELTLAR 3 1111 ALEEANADLEVK 312 APSTYGGGLSVSSSR 11 3 TKYETELNLR 11 3 VTMQNLNDR 3 11 ASLENSLEETK 12 EVATNSELVQSGK 2 1 GSCGIGGGIGGGSSR 11 LASYLDKVR 1 1 TRLEQEIATYR 2 ADLEMQIESLK 1 GQVGGDVNVEMDAAPGVDLSR 1 ILNEMRDQYEK 1 LAADDFR 1 LASYLDK 1 TMQNLEIELQSQLSMK 1 Total 17 2 3 16 3 5

Keratin type I cytoskeletal 9 K1C9_HUMAN TLLDIDNTR 1 6 4 3314 513 VQALEEANNDLENK 14314 61511 SGGGGGGGLGSGGSIR 6 42 6 113 HGVQELEIELQSQLSK 2 3 12 261 FSSSSGYGGGSSR 5314 3 IKFEMEQNLR 15 1 16 QGVDADINGLR 312 211 QVLDNLTMEK 1211 111 1 STMQELNSR 31311 GGSGGSYGGGGSGGGYGGGSGSR 3111 1 QEIECQNQEYSLLLSIK 11 14 QEYEQLIAK 31 2 TLNDMRQEYEQLIAK 213 EIETYHNLLEGGQEDFESSGAGK 21 1 SDLEMQYETLQEELMALK 12 LASYLDK 11 NHKEEMSQLTGQNSGDVNVEINVAPGK 11 SDLEMQYETLQEELMALKK 2 DQIVDLTVGNNK 1 IQDWYDKK 1 LASYLDKVQALEEANNDLENK 1 NYSPYYNTIDDLKDQIVDLTVGNNK 1 YCGQLQMIQEQISNLEAQITDVR 1 Total 639222215229839513 Supplemental Table 5 59 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin

Keratin type II K22E_HUMAN FLEQQNQVLQTK 3 1 2 214211 1 IEISELNR 2 1 21314211 LNDLEEALQQAK 3 1 1131411 NVQDAIADAEQR 1 1121311 VDLLNQEIEFLK 1 1 142 1 YEELQVTVGR 1 1 11 21 LALDVEIATYR 1 131 NLDLDSIIAEVK 111111 SISISVAGGGGGFGAAGGFGGR 1 111 1 YEDEINKR 32 GFSSGSAVVSGGSR 22 TAAENDFVTLKK 111 1 NKLNDLEEALQQAK 12 TSQNSELNNMQDLVEDYKK 111 LLEGEECR 2 STSSFSCLSR 2 YLDGLTAER 11 AQYEEIAQR 1 GGSISGGGYGSGGGK 1 GSSSGGGYSSGSSSYGSGGR 1 HGGGGGGFGGGGFGSR 1 HSSGGGSRGGSSSGGGYGSGGGGSSSVK 1 TAAENDFVTLK 1 Total 18 6 8 7 20 9 29 18 5 5 3

Keratin type II cuticular Hb1 AN,KRT86_HUMAN LLEGEEQR 1 LTAEVENAK 1 Total 2

Keratin type II cytoskeletal 1 K2C1_HUMAN FLEQQNQVLQTK 4 9 7 373729363 1 YEELQITAGR 2 7 6 343939222 QISNLQQSISDAEQR 3 4 5 155537342 1 LNDLEDALQQAK 2 6 5 245817231 TNAENEFVTIK 1 7 4 33461633 SLDLDSIIAEVK 3 1 5 233239321 1 SLNNQFASFIDK 2 3 4 153323241 IEISELNR 4 2 232414312 WELLQQVDTSTR 1 1 4 1231283 FSSCGGGGGSFGAGGGFGSR 1 6 34 181 TNAENEFVTIKK 1 5 11 26312 1 NMQDMVEDYR 4 3 123114 21 TLLEGEESR 63 6 22 THNLEPYFESFINNLR 1 1 112 17211 NKLNDLEDALQQAK 4 3 162 1 LALDLEIATYR 2 2 2261 1 SKAEAESLYQSK 5 132112 SLNNQFASFIDKVR 4125 1 AEAESLYQSK 51 3 11 SGGGFSSGSAGIINYQR 12141 MSGECAPNVSVSVSTSHTTISGGGSR 21 3 SKAEAESLYQSKYEELQITAGR 1113 SLVNLGGSKSISISVAR 321 YEDEINKR 33 DYQELMNTK 1 1 111 SLVNLGGSK 2 11 KQISNLQQSISDAEQR 12 DVDGAYMTK 1 1 GENALKDAK 1 Supplemental Table 5 60 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin GGSGGGGGGSSGGRGSGGGSSGGSIGGR 1 KDVDGAYMTK 1 NKYEDEINKR 1 SEIDNVKK 1 SISISVAR 1 Total 22 71 69 26 55 45 65 31 118 36 35 28 6

Keratin type II cytoskeletal 5 K2C5_HUMAN ISISTSGGSFR 111 YEELQQTAGR 3 LAELEEALQK 2 LLEGEECR 2 TTAENEFVMLK 2 YEDEINKR 2 FASFIDKVR 1 HEISEMNR 1 LALDVEIATYR 1 NLDLDSIIAEVK 1 NMQDLVEDFK 1 QCANLQNAIADAEQR 1 SFSTASAITPSVSR 1 VSLAGACGVGGYGSR 1 Total 14 3 3 2

Keratin type II cytoskeletal 6 K2C6A_HUMAN ISIGGGSCAISGGYGSR 3 WTLLQEQGTK 21 ADTLTDEINFLR 2 AIGGGLSSVGGGSSTIK 2 LALDVEIATYR 2 NKLEGLEDALQK 2 NLDLDSIIAEVK 11 QNLEPLFEQYINNLR 2 TAAENEFVTLK 11 YEDEINKR 11 YEELQVTAGR 2 AQYEEIAQR 1 FLEQQNKVLETK 1 GMQDLVEDFK 1 GRLDSELR 1 GSGGLGGACGGAGFGSR 1 LLEGEECR 1 NKYEDEINKR 1 QCANLQAAIADAEQR 1 QEIAEINR 1 QLDSIVGER 1 TAAENEFVTLKK 1 Total 5 4 21 5

Lamin-A/C LMNA_HUMAN SNEDQSMGNWQIK 113 4 TALINSTGEEVAMR 123 3 AGQVVTIWAAGAGATHSPPTDLVWK 13 3 VAVEEVDEEGKFVR 113 2 QNGDDPLLTYR 12 AQNTWGCGNSLR 11 VAVEEVDEEGK 2 AAYEAELGDAR 1 IDSLSAQLSQLQK 1 LADALQELR 1 LKDLEALLNSK 1 LQEKEDLQELNDR 1 Supplemental Table 5 61 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin LQTMKEELDFQK 1 MQQQLDEYQELLDIK 1 NSNLVGAAHEELQQSR 1 RQNGDDPLLTYR 1 SGAQASSTPLSPTR 1 SSFSQHAR 1 SVGGSGGGSFGDNLVTR 1 Total 31016 22

LOXL LOXL1_HUMAN GLPDLVPDPNYVQASTYVQR 1 YIVLESDFTNNVVR 1 Total 2

LTBP-2 LTBP2_HUMAN STPLGQQQPAPR 11 EQDAPVAGLQPVER 1 RPGGSYPAAAAAK 1 SGAAPPTPPR 1 SLGPGTCTLPLAQR 1 Total 24

Lysyl oxidase LYOX_HUMAN DPGAAVPGAANASAQQPR 2 TPILLIR 2 VDGMVGDDPYNPYK 2 CAAEENCLASTAYR 1 HWFQAGYSTSR 1 Total 8

MAGP-1 MFAP2_HUMAN TVCAHEELLR 6 LYSIHRPCK 5 EEQYPCTR 4 QCLNEVCFYSLR 3 RVYVINK 3 CGVMASSGLCQSVAASCAR 1 Total 22

Matrilin-2 MATN2_HUMAN ADLVFIIDSSR 1 THPQTALLESSCENKR 1 VGLLQYGSTVK 1 Total 3

Myosin-9 MYH9_HUMAN QLEEAEEEAQR 1211 ALEQQVEEMK 11 ANLQIDQINTDLNLER 11 IAQLEEQLDNETK 11 LQQELDDLLVDLDHQR 2 DELADEIANSSGK 1 DLEAHIDSANK 1 ELEDATETADAMNR 1 EQLEEEEEAKHNLEK 1 IAQLEEELEEEQGNTELINDR 1 LQVELDNVTGLLSQSDSK 1 NAEQYKDQADK 1 TQLEELEDELQATEDAK 1 Total 3772 2

Neutrophil defensin 13 MAN,DEF3_HUMAN IPACIAGER 1 YGTCIYQGR 1 Total 2

Supplemental Table 5 62 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin Obscurin OBSCN_HUMAN DGRTLSPGPKYEVQASAGRR 1 EGATAVLQCELNSAAPVEWRKGSETLR 1 Total 2

Perlecan PGBM_HUMAN AGLSSGFIGCVR 2342 LHHVSPADSGEYVCR 2233 1 LPAVEPTDQAQYLCR 153 1 LDVEFKPLAPDGVLLFSGGK 224 1 LHQMSVADSGEYVCR 2 3 111 1 YELGSGLAVLR 21 42 AAGVPSATITWR 122111 LEGDTLIIPR 132 1 1 ASYAQQPAESR 31 1 2 EVSEAVVDTLESEYLK 121 12 LPQVSPADSGEYVCR 131 1 1 LSGSHSQGVAYPVR 122 1 1 SPAYTLVWTR 42 1 AVTLECVSAGEPR 132 IQVVVLSASDASPPPVK 122 1 LLSGPYFWSLPSR 21 2 1 SIEYSPQLEDAGSR 11211 AFAHLQVPER 13 1 CLCLPGFSGPR 23 GHTPTQPGALNQR 212 GMVFGIPDGVLELVPQR 2111 IAHVELADAGQYR 1111 1 LHLVSPADSGEYVCR 122 LYIFQASPADAGQYVCR 12 11 LYQASPADSGEYVCR 31 1 VGGHLRPGIVQSGGVVR 221 VTSYGGELR 31 1 ALEVEECR 31 EGGQLPPGHSVQDGVLR 4 GPSCQDCDTGYTR 31 IESSSPTVVEGQTLDLNCVVAR 121 ITFRPDSADGMLLYNGQK 211 RVPGSPTNLANR 31 SAEPLALGR 31 SGPVEDFVSLAMVGGHLEFR 211 SIGASVEFHCAVPSDR 112 SQSVRPGADVTFICTAK 22 TCESLGAGGYR 21 1 TPSGLYLGTCER 31 AELLVTEAPSKPITVTVEEQR 21 CLIHDGAAPISLEWK 11 1 DGFKGDLCEHEENPCQLR 111 EHLLMALAGIDTLLIR 11 1 FDAGSGMATIR 21 GGSLPSHHQTR 21 IPGDQVVSVVFIK 21 IPQVTPADSGEYVCHVSNGAGSR 2 1 IQNLDQSCQGTYICQAHGPWGK 21 LCNECADGSFHLSTR 111 LGTVPQFPR 21 LLQVTPADSGEYVCR 21 MASVGLSDIAMDTTVTHATSHGR 111 NELLHFER 21 RGGSLPAR 3 VAEGQTLDLK 21 Supplemental Table 5 63 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin VDSYGGSLR 21 VTVTSEGGR 21 VVGSSGTQEASVLVTIQQR 11 1 AAGVPSATITWRKEGGSLPPQARSER 2 AMDFNGILTIR 11 AQIHGGILR 11 ATATSCRPCPCPYIDASR 2 AVLHVHGGGGPR 2 CEEGVTVTTPSLSGAGSYLALPALTNTHHELR 11 CRPVNQEIVR 11 CSATGSPAPTIHWSK 11 EGGSLPPQAR 11 EHLLMALADLDELLIR 11 EQAWQRPDGQPATR 2 ESDQGAYTCEAMNAR 11 FLVHDSFWALPEQFLGNK 2 GDLCEHEENPCQLR 11 GGSLPPHTQVHGSR 11 GMLEPVQRPDVVLVGAGYR 2 HPTPLALGHFHTVTLLR 11 HQTHGSQLR 2 IESSSSHVAEGETLDLNCVVPGQAHAQVTWHK 11 LDGSLPPDSR 11 QPDFISFGLVGGRPEFR 11 QPQAIITWYK 11 RGGSLPSR 2 RLDLTYSFLGSQGIGQCYDSSPCER 11 SIVPQGGSHSLR 11 SPVISIDPPSSTVQQGQDASFK 2 VGSSLPGR 11 VISSGSVASYVTSPQGFQFR 11 AELLQVLQSLEAVLIQTVYNTK 1 CEHSQALHCHPEACGPDATCVNRPDGR 1 CSATGSPTPTLEWTGGPGGQLPAK 1 CVSTNMCIPASFHCDEESDCPDR 1 EDGRPVPSGTQQR 1 ETSLIVTIQGSGSSHVPSVSPPIR 1 FQGLDLNEELYLGGYPDYGAIPK 1 GCVGEVSVNGK 1 GQTVTFTCVAIGVPTPIINWR 1 GSMGTSGEACR 1 HLISTHFAPGDFQGFALVNPQR 1 HQGSELHFPSVQPSDAGVYICTCR 1 HQTHGSLLR 1 LRIPQVTPADSGEYVCHVSNGAGSR 1 LRLPQVSPADSGEYVCR 1 QVQFSEEHWVHESGRPVQR 1 RGGNLPAR 1 RGGSLPPHTQVHGSR 1 RPEEVCGPTQFR 1 SPGPNVAVNAKGSVYIGGAPDVATLTGGR 1 SPLPWQHR 1 VPGSPTNLANR 1 Total 20 8 23 153 104 4 13 25 2 15

Plasminogen activator inhibitor 1 PAI1_HUMAN QFQADFTSLSDQEPLHVAQALQK 1 673 4 33 FIINDWVK 1 453115 32 HNPTGTVLFMGQVMEP 121552 3 33 Supplemental Table 5 64 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin EVPLSALTNILSAQLISHWK 1 271 16 42 VFQQVAQASK 3 5413 2 42 KPLENLGMTDMFR 1 2 253 16 21 QVDFSEVER 2 5413 1 22 1 TPFPDSSTHR 1 3512 2 43 ELMGPWNKDEISTTDAIFVQR 1 1 441 15 11 FSLETEVDLR 1 3 141114 1 SDGSTVSVPMMAQTNK 1 1 2431 4 1 LVQGFMPHFFR 1 2 142 13 11 MAPEEIIMDRPFLFVVR 441 2 21 1 IEVNESGTVASSSTAVIVSAR 1 3 12 13 1 1 DEISTTDAIFVQR 1 2 12111 GAVDQLTR 132 2 1 GMISNLLGK 22131 LVLVNALYFNGQWK 1 1 12 13 GNMTRLPRLLVLPKFSLETEVDLR 21 VKIEVNESGTVASSSTAVIVSAR 12 IDDKGMAPALR 1 LPRLLVLPKFSLETEVDLR 1 Total 8 18 9 52 70 26 12 8 62 32 29 5

Plexin domain-containing protein 2 PXDC2_HUMAN EITVATGGFIYTGEVVHR 11 EIPVLVTQISSTNHPVK 1 VGLSDAFVVVHR 1 Total 22

Probable JmjC domain-containing histone demethylation protein 2C JHD2C_HUMAN GLEGERENYSRVASSSSSPKSHIIK 1 KGSDSSIPDEEK 1 Total 2

Proepithelin GRN_HUMAN EVVSAQPATFLAR 11315 AVALSSSVMCPDAR 12 6 VHCCPHGAFCDLVHTR 11 5 APAHLSLPDPQALKR 5 CITPTGTHPLAK 14 QHCCPAGYTCNVK 4 GSEIVAGLEK 12 LPAHTVGDVK 11 1 SCEKEVVSAQPATFLAR 3 CDMEVSCPDGYTCCR 2 CPDGSTCCELPSGK 11 GSEIVAGLEKMPAR 2 GTCEQGPHQVPWMEK 2 QGWACCPYR 11 APAHLSLPDPQALK 1 DVECGEGHFCHDNQTCCR 1 HCCPAGFR 1 SRCPDGSTCCELPSGK 1 WDAPLRDPALR 1 Total 348248

Prolactin-inducible protein PIP_HUMAN FYTIEILKVE 1 TVQIAAVVDVIR 1 TYLISSIPLQGAFNYK 1 Total 3

Supplemental Table 5 65 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin Protein CYR61 CYR61_HUMAN GLECNFGASSTALK 11 CAPGVGLVR 1 ELGFDASEVELTR 1 ILYNPLQGQK 1 Total 32

Protein disulfide isomerase A6 PDIA6_HUMAN GSTAPVGGGAFPTIVER 1 LAAVDATVNQVLASR 1 Total 2

Protein mobA MOBA_THIFE MIALSQEAVRSKDTINHYVLSWR 1 TGSGATLFVGDVGVKASSADRDASLSKLQK 1 Total 2

Protein NOV homolog NOV_HUMAN TIQAEFQCSPGQIVK 111111 LCMVRPCEQEPEQPTDKK 111 NNEAFLQELELK 111 DGQIGCVPR 11 KPVMVIGTCTCHTNCPK 11 KVEVPGECCEK 11 CQLDVLLPEPNCPAPR 1 FCGVCSDGR 1 QRGESCSDLEPCDESSGLYCDR 1 Total 3 2 2345 2

Protein S100-A7 S10A7_HUMAN GTNYLADVFEK 11 21 ENFPNFLSACDK 11 2 SIIGMIDMFHK 21 ENFPNFLSACDKK 1 KIDFSEFLSLLGDIATDYHK 1 Total 32 72

Protein S100-A8 S10A8_HUMAN ALNSIIDVYHK 1 LLETECPQYIR 1 Total 2

Protein S100-A9 S10A9_HUMAN NIETIINTFHQYSVK 11 KDLQNFLK 1 LGHPDTLNQGEFK 1 VIEHIMEDLDTNADK 1 Total 23

Protocadherin-17 PCD17_HUMAN ASRDSSEMGAVLEQLDHPNR 1 EIDKLLQDCRGNDPVAVRK 1 Total 2

Retinoic acid receptor responder protein 2 RARR2_HUMAN EAEEHQETQCLR 1 3 111 GLQVALEEFHK 12 21 LVHCPIETQVLR 31 1 HPPVQWAFQETSVESAVDTPFPAGIFVR 1 RGLQVALEEFHK 1 Total 2 9 243

RUN domain-containing protein 2A RUN2A_HUMAN EITASSAVSILIKPEQETDPLPVVSR 1 FAPTVSDLLKESTQNVTSLLK 1 Total 2

Supplemental Table 5 66 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin Ryanodine receptor 3 RYR3_HUMAN CAPEMHLIQTGKGEAIRIR 1 TKALSMLTEAVQCSGAHIR 1 Total 2

Serine/threonine-protein kinase SMG1 SMG1_HUMAN KAVEHNIQIGKFSQLVMNR 1 LLPNMLSPDPRELQKSIEVQLLR 1 Total 2

Serum albumin ALBU_HUMAN KVPQVSTPTLVEVSR 161 41 CCTESLVNR 14 3 YLYEIAR 161 YICENQDSISSK 13 2 CASLQKFGER 4 VPQVSTPTLVEVSR 11 2 DVFLGMFLYEYAR 1 DVFLGMFLYEYARR 1 FKDLGEENFK 1 LVNEVTEFAK 1 RHPDYSVVLLLR 1 TCVADESAENCDK 1 Total 24 245 122

Spectrin beta chain brain 1 SPTB2_HUMAN EAVCEVALDYK 2 LNDGNEYLFQAK 2 TAASGIPYHSEVPVSLK 1 Total 5

Stanniocalcin-2 STC2_HUMAN VIVEMIHFK 2 3 143 3343 GLHGICMTFLHNAGK 3 2231 213 HDLCAAAQENTR 1 3211 1223 DLLLHEPYVDLVNLLLTCGEEVK 21 331 AHHGEAGHHLPEPSSR 1 1211 2 EMVSQLQR 31 22 SFIKDALK 1 Total 4 6101493 613147

Striated muscle preferentially expressed protein kinase SPEG_HUMAN LSALGRSPR 1 SRAIQECRSPVPPPAADPPEARTK 1 Total 2

Sulfhydryl oxidase 1 QSOX1_HUMAN NNEEYLALIFEK 1112 DFNIPGFPTVR 11 11 EVALDLSQHK 1 111 LAGAPSEDPQFPK 11 11 FGVTDFPSCYLLFR 111 IYMADLESALHYILR 12 LDVPVWDVEATLNFLK 111 LEEIDGFFAR 111 NGSGAVFPVAGADVQTLR 111 SALYSPSDPLTLLQADTVR 11 1 VLNTEANVVR 12 AAPGQEPPEHMAELQR 2 AHFSPSNIILDFPAAGSAAR 11 AWRPALYLAALDCAEETNSAVCR 11 DCASHFEQMAAASMHR 11 FPVLEGQR 11 IEVGRFPVLEGQR 11 Supplemental Table 5 67 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin KVNWIGCQGSEPHFR 11 SFYTAYLQR 11 VNWIGCQGSEPHFR 11 VPVLMESR 11 CNSGSGPPPSLLLLLLWLLAVPGANAAPR 1 DVQNVAAAPELAMGALELESR 1 ELCSACHNER 1 GFPCSLWVLFHFLTVQAAR 1 GYVHYFFGCR 1 KFGVTDFPSCYLLFR 1 RVLNTEANVVR 1 VGSPNAAVLWLWSSHNR 1 Total 2 6 76331722

Tenascin TENA_HUMAN SQTVSAIATTAMGSPK 11 ASVTGYLLVYESVDGTVK 1 ITYVPITGGTPSMVTVDGTK 1 KQSEPLEITLLAPER 1 Total 23

TGF beta-2 TGFB2_HUMAN IEQLSNMIVK 1 212 FAGIDGTSTYTSGDQK 1 112 ALDAAYCFR 111 TPHLLLMLLPSYR 12 AEGEWLSFDVTDAVHEWLHHK 11 FDVSAMEK 11 ERSDEEYYAK 1 GYNANFCAGACPYLWSSDTQHSR 1 IELYQILK 1 SKDLTSPTQR 1 Total 65410

Thrombospondin-1 TSP1_HUMAN FVFGTTPEDILR 311 23 LCNNPTPQFGGK 231 11 IPESGGDNSVFDIFELTGAAR 11 13 TIVTTLQDSIR 111 21 DCVGDVTENQICNK 11 21 GGVNDNFQGVLQNVR 221 IEDANLIPPVPDDKFQDLVDAVR 11 2 MENAELDVPIQSVFTR 13 NALWHTGNTPGQVR 112 QHVVSVEEALLATGQWK 22 QVTQSYWDTNPTR 211 AGTLDLSLTVQGK 21 CENTDPGYNCLPCPPR 21 DLQAICGISCDELSSMVLELR 111 FTGSQPFGQGVEHATANK 12 SITLFVQEDR 21 CNYLGHYSDPMYR 11 DDDYAGFVFGYQSSSR 11 DNCQYVYNVDQR 11 KVTEENKELANELR 2 NNEEWTVDSCTECHCQNSVTICK 11 AQLYIDCEK 1 FYVVMWK 1 GFLLLASLR 1 GTSQNDPNWVVR 1 IMADSGPIYDK 1 KDHSGQVFSVVSNGK 1 Supplemental Table 5 68 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin KIMADSGPIYDK 1 Total 8 11 6 3 27 37

Titin TITIN_HUMAN ATLTWTPPLEDGGSPIKSYILEK 1 EAPPKARVPEEPKRAVPEEK 1 EISIEAMDFVDRLLVKERKSR 1 ESEFVRFSKTENKITLSIK 1 FESVSADQMTLSWFPPKDDGGSK 1 IYAMNSEGLGEPALVPGTPKAEDR 1 KPEAPPAKVPEVPKEVVPEKKVAVPK 1 LKIGLITKNTVHLSWKPPK 1 NAAGVFSEPSESTGAITARDEVDPPR 1 NNVATLVFNQVDINDSGEYICK 1 QTGECKLVISMTFADDAGEYTIVVR 1 RDDKTLESGDKYDVIADGK 1 SKEEHER 1 TEEGYYEAITAVELK 1 VKVMDVPGPPKDLKVSDITRGSCR 1 VPEVPKEVVPEKKVAVPKKPEVPPAK 1 Total 23 623

Trinucleotide repeat-containing gene 6A protein TNR6A_HUMAN NSGSSSSLNTTLPSTSAWSSIR 1 TDNGTEAWGSSATQTFNSGACIDK 1 Total 2

Tyrosine-protein kinase BTK BTK_HUMAN YPVSQQNK 1 YPVSQQNKNAPSTAGLGYGSWEIDPK 1 Total 2

Uncharacterized protein C6orf174 CF174_HUMAN EGGAPQPPPPRGWR 1 TGSRTGPPASIRGGGGGAEKATPLAPK 1 Total 2

Vacuolar protein sorting- associated protein VP13D_HUMAN AQSPVSGPNVAHLTDGATLNDR 1 LLAESLPR 1 SLPSHMEEAPNVFQLYQRPTSASRK 1 YFNKLKGGLLSTDDKEEMCR 1 Total 22

Vacuolar protein sorting- associated protein 13A VP13A_HUMAN LFFILLTPVNHSTADKIPLTKVGRR 1 VPEYKTVISFHSKDQLNITLSK 1 Total 2

Vimentin VIME_HUMAN DGQVINETSQHHDDLE 1 EEAENTLQSFR 1 EMEENFAVEAANYQDTIGR 1 ETNLDSLPLVDTHSK 1 FADLSEAANR 1 ILLAELEQLK 1 ILLAELEQLKGQGK 1 KVESLQEEIAFLK 1 LLEGEESR 1 LQEEMLQREEAENTLQSFR 1 NLQEAEEWYK 1 SLYASSPGGVYATR 1 Supplemental Table 5 69 Defining the elastic fiber interactome Calsyn- SwissProt Entry tenin-1 Pro- Protein Identity Name Peptide sequence Ex Fibulin 5 LOX MAGP-1 PF1 PF11 PF12 PF13 PF2 PF5 PF7 PF8 Epithelin VELQELNDR 1 Total 11 2

Zinc transporter ZIP10 S39AA_HUMAN GHQDLDPDNEGELR 1 LLTNLGLGER 1 Total 2

Zinc-alpha-2-glycoprotein ZA2G_HUMAN AGEVQEPELR 1 AREDIFMETLK 1 EIPAWVPFDPAAQITK 1 HVEDVPAFQALGSLNDLQFFR 1 NILDRQDPPSVVVTSHQAPGEK 1 QDPPSVVVTSHQAPGEK 1 QKWEAEPVYVQR 1 YSLTYIYTGLSK 1 YYYDGKDYIEFNK 1 Total 9

Supplemental Table 5 70 Defining the elastic fiber interactome Part 2 coll a-1 coll a-1 coll a-2 SwissProt Entry (VIII) (VIII) (VIII) Protein Identity Name Peptide sequence NC2 FL NC2 Annexin A2 ANXA2_HUMAN GVDEVTIVNILTNR 1 QDIAFAYQR 1 SALSGHLETVILGLLK 1 TDLEKDIISDTSGDFR 1 TPAQYDASELK 1 Total 2 3

Collagen alpha-1(I) chain CO1A1_HUMAN VLCDDVICDETK 3 2 DGEAGAQGPPGPAGPAGER 3 1 GETGPAGPAGPVGPVGAR 2 2 ALLLQGSNEIEIR 2 SGEYWIDPNQGCNLDAIK 2 GFPGADGVAGPK 1 GVPGPPGAVGPAGK 1 GVQGPPGPAGPR 1 NSVAYMDQQTGNLK 1 SGDRGETGPAGPAGPVGPVGAR 1 SLSQQIENIR 1 Total 14 9

Collagen alpha-1(VIII) chain CO8A1_HUMAN EMPHLQYGK 1 EYPHLPQYMK 1 Total 2

Collagen alpha-2(I) chain CO1A2_HUMAN GETGPSGPVGPAGAVGPR 1 GPPGESGAAGPTGPIGSR 1 Total 2

Collagen alpha-2(VIII) chain CO8A2_HUMAN GEPGPPGKPGPR 3 GQYLEMPLPLLPMDLK 3 YIQPMQK 2 Total 8

Fibrillin-1 FBN1_HUMAN CECFPGLAVGLDGR 1 CSNQLPQSITK 1 GFIYKPDLK 1 Total 3

Fibronectin FINC_HUMAN VPGTSTSATLTGLTR 3 2 EESPLLIGQQSTVSDVPR 1 1 ISCTIANR 1 1 CDPHEATCYDDGK 1 FLATTPNSLLVSWQPPR 1 FTNIGPDTMR 1 GATYNIIVEALK 1 GDSPASSKPISINYR 1 HYQINQQWER 1 LLCQCLGFGSGHFR 1 QYNVGPSVSK 1 RPGGEPSPEGTTGQSYNQYSQR 1 SSPVVIDASTAIDAPSNLR 1 STTPDITGYR 1 SYTITGLQPGTDYK 1

Supplemental Table 5 71 Defining the elastic fiber interactome coll a-1 coll a-1 coll a-2 SwissProt Entry (VIII) (VIII) (VIII) Protein Identity Name Peptide sequence NC2 FL NC2 TEIDKPSQMQVTDVQDNSISVK 1 TFYSCTTEGR 1 TYHVGEQWQK 1 VTWAPPPSIDLTNFLVR 1 WCHDNGVNYK 1 WSRPQAPITGYR 1 Total 15 6 6

Histone H2B H2B1M_HUMAN ESYSVYVYK 1 KESYSVYVYK 1 Total 2

Histone H4. H4_HUMAN DAVTYTEHAK 4 ISGLIYEETR 4 DNIQGITKPAIR 2 DNIQGITKPAIRR 2 Total 12

Hornerin HORN_HUMAN GPYESGSGHSSGLGHR 1 GPYESGSGHSSGLGHRESR 1 GSGSGQSPSSGQHGTGFGR 1 Total 3

Keratin type I cytoskeletal 10 K1C10_HUMAN GSLGGGFSSGGFSGGSFSR 2 1 LENEIQTYR 1 2 NQILNLTTDNANILLQIDNAR 1 2 VLDELTLTK 1 2 VTMQNLNDR 2 1 AETECQNTEYQQLLDIK 1 1 ALEESNYELEGK 2 DAEAWFNEK 2 ELTTEIDNNIEQISSYK 1 1 GSSGGGCFGGSSGGYGGLGGFGGGSFR 1 1 IRLENEIQTYR 1 1 NVQALEIELQSQLALK 1 1 QSLEASLAETEGR 1 1 HYSSSRSGGGGGGGGCGGGGGVSSLR 1 ISSSKGSLGGGFSSGGFSGGSFSR 1 LASYLDKVR 1 NVSTGDVNVEMNAAPGVDLTQLLNNMR 1 QSVEADINGLR 1 SQYEQLAEQNR 1 Total 17 9 11

Keratin type I cytoskeletal 14 K1C14_HUMAN TRLEQEIATYR 1 VLDELTLAR 1 Total 2

Keratin type I cytoskeletal 9 K1C9_HUMAN FSSSSGYGGGSSR 1 2 1 QEYEQLIAK 1 2 STMQELNSR 1 2 VQALEEANNDLENK 2 1 HGVQELEIELQSQLSK 2 QGVDADINGLR 1 1 SGGGGGGGLGSGGSIR 1 1

Supplemental Table 5 72 Defining the elastic fiber interactome coll a-1 coll a-1 coll a-2 SwissProt Entry (VIII) (VIII) (VIII) Protein Identity Name Peptide sequence NC2 FL NC2 TLLDIDNTR 2 EIETYHNLLEGGQEDFESSGAGK 1 GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSR 1 GGSGGSHGGGSGFGGESGGSYGGGEEASGSGGGYG 1 GSRGGSGGSYGGGGSGGGYGGGSGSR 1 IGLGGRGGSGGSYGR 1 IKFEMEQNLR 1 LASYLDKVQALEEANNDLENK 1 QEIECQNQEYSLLLSIK 1 QVLDNLTMEK 1 SDLEMQYETLQEELMALKK 1 TLNDMRQEYEQLIAK 1 Total 21 9 2

Keratin type II K22E_HUMAN FLEQQNQVLQTK 2 1 VDLLNQEIEFLK 2 1 IEISELNR 1 1 LNDLEEALQQAK 1 1 NVQDAIADAEQR 1 1 YEELQVTVGR 2 YLDGLTAER 1 1 AQYEEIAQR 1 GFSSGSAVVSGGSR 1 HGGGGGGFGGGGFGSR 1 LALDVEIATYR 1 NLDLDSIIAEVK 1 SKEEAEALYHSK 1 Total 15 7

Keratin type II cytoskeletal 1 K2C1_HUMAN SLDLDSIIAEVK 354 YEELQITAGR 343 FLEQQNQVLQTK 315 THNLEPYFESFINNLR 413 WELLQQVDTSTR 332 IEISELNR 132 TLLEGEESR 123 LNDLEDALQQAK 221 QISNLQQSISDAEQR 23 SLNNQFASFIDK 32 AEAESLYQSK 12 TNAENEFVTIK 21 TNAENEFVTIKK 12 DVDGAYMTK 11 FSSCGGGGGSFGAGGGFGSR 11 LALDLEIATYR 11 NMQDMVEDYR 11 SGGGFSSGSAGIINYQR 2 SLNNQFASFIDKVR 2 DYQELMNTK 1

GGGGGGYGSGGSSYGSGGGSYGSGGGGGGGR 1 MSGECAPNVSVSVSTSHTTISGGGSR 1 RVDQLKSDQSRLDSELK 1 SGGGFSSGSAGIINYQRR 1 SKAEAESLYQSK 1

Supplemental Table 5 73 Defining the elastic fiber interactome coll a-1 coll a-1 coll a-2 SwissProt Entry (VIII) (VIII) (VIII) Protein Identity Name Peptide sequence NC2 FL NC2 THNLEPYFESFINNLRR 1 Total 43 28 31

Keratin type II cytoskeletal 5 K2C5_HUMAN ISISTSGGSFR 1 NLDLDSIIAEVK 1 SLYNLGGSKR 1 Total 3

Keratin type II cytoskeletal 6 K2C6A_HUMAN ADTLTDEINFLR 1 GSGGLGGACGGAGFGSR 1 QNLEPLFEQYINNLR 1 WTLLQEQGTK 1 Total 4

Lamin-A/C LMNA_HUMAN TALINSTGEEVAMR 32 VAVEEVDEEGKFVR 31 ITESEEVVSR 3 LLEGEEER 3 QNGDDPLLTYR 21 SNEDQSMGNWQIK 21 DLEALLNSK 2 EDLQELNDR 2 LADALQELR 2 SLETENAGLR 2 VAVEEVDEEGK 2 AAYEAELGDAR 1 AGQVVTIWAAGAGATHSPPTDLVWK 1 AQNTWGCGNSLR 1 DLEDSLAR 1 EGDLIAAQAR 1 KLLEGEEER 1 LEAALGEAKK 1 LQEKEDLQELNDR 1 NSNLVGAAHEELQQSR 1 SNEDQSMGNWQIKR 1 Total 9302

N-acetylmuramic acid 6- phosphate etherase MURQ_SHIDS MQLEKMITEGSNAASAEIDR 1 NLDAAEAKKRLDQHGGFIR 1 Total 2

Perlecan PGBM_HUMAN CLIHDGAAPISLEWK 1 IPGDQVVSVVFIK 1 LHHVSPADSGEYVCR 1 Total 3

Plasminogen activator inhibitor 1 PAI1_HUMAN KPLENLGMTDMFR 1 VFQQVAQASK 1 Total 2

Serum albumin ALBU_HUMAN KVPQVSTPTLVEVSR 1 VPQVSTPTLVEVSR 1 Total 2

Supplemental Table 5 74 Defining the elastic fiber interactome coll a-1 coll a-1 coll a-2 SwissProt Entry (VIII) (VIII) (VIII) Protein Identity Name Peptide sequence NC2 FL NC2

Stanniocalcin-2 STC2_HUMAN HDLCAAAQENTR 1 VIVEMIHFK 1 Total 2

Tenascin-X TENX_HUMAN GTVQDLERHLRAHGYPLR 1 MHLYGLHEGQRVGPVSAVGVTAPK 1 Total 2

THAP domain-containing protein 4 THAP4_HUMAN ATGGVRGHSSAATSR 1 KDASKATGGVRGHSSAATSR 1 Total 2

Titin TITIN_HUMAN VMAVNK 1 VSDVTK 1 Total 2

Vimentin VIME_HUMAN DGQVINETSQHHDDLE 2 DNLAEDIMR 2 FADLSEAANR 2 LGDLYEEEMR 2 LLEGEESR 2 LQDEIQNMK 2 LQEEMLQR 2 QDVDNASLAR 2 QQYESVAAK 2 VELQELNDR 2 EEAENTLQSFR 1 MSTRSVSSSSYRR 1 QVDQLTNDK 1 Total 23

Supplemental Table 5 75 Defining the elastic fiber interactome

SUPPLEMENTAL TABLE 6 Gold Standard Positive Interaction Set

Table showing the complete Gold Standard (GS) dataset for published interactions with proteins identified in this study. Shown is the Protein Identity and Gene Symbol for each protein, and the type of experimental system used to identify the interaction. Also shown is the first Author and Pubmed ID number of each publication.

Gene Gene EXPERIMENTAL Protein Identity A Protein Identity B Symbol A Symbol B SYSTEM SOURCE Pubmed-ID Bullous pemphigoid antigen 1 isoforms 150 kDa -associated polypeptide 1/2/3/4/5/8 DCTN1 DST Affinity Capture-Western Liu JJ (2003) 14581450 Bullous pemphigoid antigen 1 isoforms 150 kDa dynein-associated polypeptide 6/9/10 DCTN1 DST Affinity Capture-Western Liu JJ (2003) 14581450 150 kDa dynein-associated polypeptide Vimentin DCTN1 VIM Affinity Capture-Western Payne C (2003) 14600259 Bullous pemphigoid antigen 1 isoforms 180 kDa bullous pemphigoid antigen 2 1/2/3/4/5/8 COL17A1 DST Co-localization Koster J (2003) 12482924 Bullous pemphigoid antigen 1 isoforms 180 kDa bullous pemphigoid antigen 2 1/2/3/4/5/8 COL17A1 DST Two-hybrid Hopkinson SB (2000) 10637308 Bullous pemphigoid antigen 1 isoforms 180 kDa bullous pemphigoid antigen 2 1/2/3/4/5/8 COL17A1 DST Two-hybrid Koster J (2003) 12482924 Bullous pemphigoid antigen 1 isoforms 180 kDa bullous pemphigoid antigen 2 6/9/10 COL17A1 DST Co-localization Koster J (2003) 12482924 Bullous pemphigoid antigen 1 isoforms 180 kDa bullous pemphigoid antigen 2 6/9/10 COL17A1 DST Two-hybrid Hopkinson SB (2000) 10637308 Bullous pemphigoid antigen 1 isoforms 180 kDa bullous pemphigoid antigen 2 6/9/10 COL17A1 DST Two-hybrid Koster J (2003) 12482924 250/210 kDa paraneoplastic pemphigus antigen Vimentin DSP VIM Reconstituted Complex Meng JJ (1997) 9261168 250/210 kDa paraneoplastic pemphigus antigen Vimentin DSP VIM Two-hybrid Meng JJ (1997) 9261168 34 kDa nucleolar scleroderma antigen Complement C1q FBL C1QBP Affinity Capture-MS Yanagida M (2004) 14583623 38 kDa Mov34 homolog Protein S100-A7 COPS5 Affinity Capture-Western Emberley ED (2003) 12702588 55 kDa leucine-rich repeat protein of articular ca Collagen alpha-1(I) chain PRELP COL1A1 Reconstituted Complex Bengtsson E (2002) 11847210 55 kDa leucine-rich repeat protein of articular ca Perlecan PRELP HSPG2 Reconstituted Complex Bengtsson E (2002) 11847210 59 kDa serine/threonine-protein kinase [SP] Protein S100-A9 ILK S100A9 Affinity Capture-MS Ewing RM (2007) 17353931 72 kDa gelatinase Alpha-2-macroglobulin MMP2 A2M Reconstituted Complex Arbelaez LF (1997) 9344465 72 kDa gelatinase Thrombospondin-1 MMP2 THBS1 Affinity Capture-Western Bein K (2000) 10900205 abundant SRC homology Annexin A2 GRB2 ANXA2 Affinity Capture-Western Buday L (1994) 7510700

Supplemental Table 6 76 Defining the elastic fiber interactome

accessory protein BAP31 Amyloid BCAP31 APP Two-hybrid Wang B (2003) 12529377 Actin-binding LIM protein 1 Vimentin ABLIM1 VIM Two-hybrid Rual JF (2005) 16189514 active transcription factor CREB Vimentin CREB1 VIM Two-hybrid Stelzl U (2005) 16169070

ADAM 12 precursor Insulin-like growth factor-binding protein 3 ADAM12 IGFBP3 Biochemical Activity Loechel F (2000) 11095942

ADAM 12 precursor Insulin-like growth factor-binding protein 5 ADAM12 IGFBP5 Biochemical Activity Loechel F (2000) 11095942 Adapter protein X11alpha Amyloid APBA1 APP Affinity Capture-Western Borg JP (1996) 8887653 Adapter protein X11alpha Amyloid APBA1 APP Reconstituted Complex Borg JP (1996) 8887653 ADP,ATP carrier protein 3 Vimentin SLC25A6 VIM Two-hybrid Stelzl U (2005) 16169070 Agrin Amyloid AGRIN APP Invitro Cotman et al. 10673326 Agrin AGRIN DAG1 Invivo Gee et al. 8205617 Agrin Heparin-binding growth factor 2 (FGF-2) AGRIN FGF2 Invitro Cotman et al. 10328953 Agrin Laminin subunit alpha-1 AGRIN LAMA1 Invivo Denzer et al. 9430625 Agrin Laminin subunit alpha-2 AGRIN LAMA2 Invitro Cotman et al. 10328953 Muscle, skeletal receptor tyrosine protein Agrin kinase AGRIN MUSK Invivo Campanelli et al. 1659950 Agrin Neural cell adhesion molecule L1 AGRIN L1CAM Invivo Cole et al. 9117266 Agrin Pleiotrophin AGRIN PTN Invitro Dagette et al. 9026315 Receptor protein-tyrosine phosphatase Agrin sigma AGRIN ptprs Invitro Aricescu et al. 11865065 Agrin Tenacin AGRIN TNC Invitro Cotman et al. 10328953 Agrin Thrombospondin-1 AGRIN THBS1 Invitro Cotman et al. 10328953 A-kinase anchor protein 9 AKAP9 CALM1 Affinity Capture-Western Takahashi M (2002) 12221128 A-kinase anchor protein 9 calmodulin 2 AKAP9 CALM2 Two-hybrid Takahashi M (2002) 12221128

A-kinase anchor protein 9 epsilon AKAP9 CSNK1E Affinity Capture-Western Sillibourne JE (2002) 12270714

A-kinase anchor protein 9 Casein kinase I isoform delta AKAP9 CSNK1D Affinity Capture-Western Sillibourne JE (2002) 12270714 A-kinase anchor protein 9 formin-binding protein 17 AKAP9 FNBP1 Two-hybrid Larocca MC (2004) 15047863 A-kinase anchor protein 9 , RII-alpha subunit AKAP9 PRKAR2A Affinity Capture-Western Takahashi M (1999) 10358086 A-kinase anchor protein 9 salt tolerator AKAP9 TRIP10 Two-hybrid Larocca MC (2004) 15047863 A-kinase anchor protein 9 serine/threonine protein kinase N AKAP9 PKN1 Reconstituted Complex Takahashi M (1999) 10358086 , gamma complex associated A-kinase anchor protein 9 protein 3 AKAP9 TUBGCP3 Affinity Capture-Western Takahashi M (2002) 12221128 Alpha-2-macroglobulin A2M IL4 Reconstituted Complex Garber TR (2000) 10714547 A disintegrin and metalloproteinase with Alpha-2-macroglobulin thrombosp A2M ADAMTS1 Affinity Capture-Western Kuno K (1999) 10373500 Alpha-2-macroglobulin Alzheimer disease 2 A2M APOE Affinity Capture-Western Krimbou L (1998) 9831625 Alpha-2-macroglobulin Alzheimer disease 2 A2M APOE Reconstituted Complex Krimbou L (1998) 9831625 Alpha-2-macroglobulin interleukin 10 A2M IL10 Reconstituted Complex Garber TR (2000) 10714547 Alpha-2-macroglobulin nerve growth factor, beta polypeptide A2M NGFB Reconstituted Complex Gonias SL (2000) 10681572

Supplemental Table 6 77 Defining the elastic fiber interactome

Alpha-2-macroglobulin obesity (murine homolog, leptin) A2M LEP Reconstituted Complex Birkenmeier G (1998) 9724081

Alpha-2-macroglobulin Phospholipid-cholesterol acyltransferase A2M LCAT Affinity Capture-Western Krimbou L (2001) 11435418

Alpha-2-macroglobulin Phospholipid-cholesterol acyltransferase A2M LCAT Reconstituted Complex Krimbou L (2001) 11435418 progestagen-associated endometrial Alpha-2-macroglobulin protein (placen A2M PAEP Co-purification Riely GJ (2000) 11023837 progestagen-associated endometrial Alpha-2-macroglobulin protein (placen A2M PAEP Reconstituted Complex Riely GJ (2000) 11023837 Alpha- cytoskeletal isoform Titin ACTN1 TTN Reconstituted Complex Joseph C (2001) 11305911

AMBP protein precursor Alpha-2-macroglobulin AMBP A2M Affinity Capture-Western Falkenberg C (1990) 1697852

AMBP protein precursor Alpha-2-macroglobulin AMBP A2M Affinity Capture-Western Falkenberg C (1994) 7519849

AMBP protein precursor Fibronectin AMBP FN1 Affinity Capture-Western Falkenberg C (1994) 7519849 AMBP protein precursor Serum albumin AMBP ALB Affinity Capture-Western Berggard T (1997) 9183005 AMPK-related protein kinase 5 [SP,EG] Protein S100-A8 ARK5 S100A8 Affinity Capture-MS Ewing RM (2007) 17353931 AMPK-related protein kinase 5 [SP,EG] Protein S100-A9 ARK5 S100A9 Affinity Capture-MS Ewing RM (2007) 17353931 Amyloid accessory protein BAP31 APP BCAP31 Co-fractionation Wang B (2003) 12529377

Amyloid Acetylcholinesterase precursor APP ACHE Reconstituted Complex Cottingham MG (2002) 12427014 Amyloid Adapter protein X11alpha APP APBA1 Affinity Capture-Western Biederer T (2002) 12196555 Amyloid Adapter protein X11alpha APP APBA1 Reconstituted Complex Biederer T (2002) 12196555 Amyloid Adapter protein X11alpha APP APBA1 Reconstituted Complex Borg JP (1996) 8887653 Amyloid Amyloid APBA2 APP Affinity Capture-Western Araki Y (2003) 12972431 Amyloid Amyloid APBA2 APP Reconstituted Complex Tomita S (1999) 9890987 Amyloid Amyloid APBA2 APP Two-hybrid Tomita S (1999) 9890987 Amyloid Amyloid APBB1 APP Affinity Capture-Western Borg JP (1996) 8887653 Amyloid Amyloid APBB1 APP Affinity Capture-Western Guenette SY (1996) 8855266 Amyloid Amyloid Apbb1 APP Affinity Capture-Western Zambrano N (1997) 9045663 Amyloid Amyloid APBB1 APP Reconstituted Complex Borg JP (1996) 8887653 Amyloid Amyloid APBB1 APP Reconstituted Complex Tanahashi H (1999) 10081969

Amyloid Amyloid APBB1 APP Reconstituted Complex Trommsdorff M (1998) 9837937 Amyloid Amyloid APBB1 APP Reconstituted Complex Zambrano N (1997) 9045663 Amyloid Amyloid Apbb1 APP Two-hybrid Bressler SL (1996) 8894693 Amyloid Amyloid APBB2 APP Affinity Capture-Western Chang Y (2003) 14527950 Amyloid Amyloid APBB3 APP Reconstituted Complex Tanahashi H (1999) 10081969 Amyloid Amyloid APP APBA2 Affinity Capture-Western Araki Y (2003) 12972431 Amyloid Amyloid APP APBA2 Affinity Capture-Western Biederer T (2002) 12196555 Amyloid Amyloid APP APBA2 Reconstituted Complex Biederer T (2002) 12196555 Amyloid Amyloid APP APBB1 Reconstituted Complex Borg JP (1996) 8887653

Supplemental Table 6 78 Defining the elastic fiber interactome

Amyloid Amyloid APP Apbb1 Reconstituted Complex Bressler SL (1996) 8894693

Amyloid Amyloid APP APBB1 Two-hybrid Trommsdorff M (1998) 9837937 Amyloid Amyloid APP APPBP1 Reconstituted Complex Chow N (1996) 8626687 Amyloid Amyloid APP HADH2 Affinity Capture-Western Yan SD (1997) 9338779 Amyloid Amyloid APP HADH2 Reconstituted Complex Yan SD (1997) 9338779 Amyloid Amyloid APP HADH2 Two-hybrid Yan SD (1997) 9338779 Amyloid Amyloid APPBP1 APP Affinity Capture-Western Chow N (1996) 8626687 Amyloid Calsyntenin-1 APBA2 CLSTN1 Affinity Capture-Western Araki Y (2003) 12972431 Amyloid Calsyntenin-1 APBA2 CLSTN1 Two-hybrid Araki Y (2003) 12972431 Amyloid Calsyntenin-1 APP CLSTN1 Affinity Capture-Western Araki Y (2003) 12972431 Amyloid Caveolin-1 APP CAV1 Co-fractionation Ikezu T (1998) 9553108 Amyloid Caveolin-1 APP CAV1 Protein-RNA Ikezu T (1998) 9553108 Amyloid Collagen alpha-1 APP COL25A1 Reconstituted Complex Hashimoto T (2002) 11927537 Amyloid dermatan sulphate proteoglycan I APP BGN Reconstituted Complex Snow AD (1995) 7793988 Amyloid Fibulin-1 APP FBLN1 Affinity Capture-Western Ohsawa I (2001) 11238726 Amyloid Fibulin-1 APP FBLN1 Two-hybrid Ohsawa I (2001) 11238726

Amyloid Liprin-alpha-3 APBB1 LRP1 Reconstituted Complex Trommsdorff M (1998) 9837937 Amyloid Neuronal Munc18-1-interacting protein 3 APP APBA3 Affinity Capture-Western Biederer T (2002) 12196555 Amyloid Neuronal Munc18-1-interacting protein 3 APP APBA3 Reconstituted Complex Biederer T (2002) 12196555 Amyloid Neuronal Munc18-1-interacting protein 3 APP APBA3 Reconstituted Complex Tanahashi H (1999) 10049767 Amyloid Neuronal Munc18-1-interacting protein 3 APP APBA3 Two-hybrid Tanahashi H (1999) 10049767 Amyloid Protein interacting with APP tail 1 APP APPBP2 Reconstituted Complex Zheng P (1998) 9843960 Amyloid Protein interacting with APP tail 1 APP APPBP2 Two-hybrid Zheng P (1998) 9843960 SHC (Src homology 2 domain containing) Amyloid transformin APP SHC1 Affinity Capture-Western Tarr PE (2002) 11877420 synuclein, alpha (non A4 component of Amyloid amyloid prec APP SNCA Reconstituted Complex Jensen PH (1997) 9163350 Amyloid synuclein, beta APP SNCB Reconstituted Complex Jensen PH (1997) 9163350 Kontrogianni- Konstantopoulos A 1, erythrocytic Obscurin ANK1 OBSCN Two-hybrid (2003) 12631729 Kontrogianni- Konstantopoulos A ankyrin 1, erythrocytic Titin ANK1 TTN Reconstituted Complex (2003) 12444090 Kontrogianni- Konstantopoulos A ankyrin 1, erythrocytic Titin ANK1 TTN Two-hybrid (2003) 12444090 ankyrin repeat domain 1 (cardiac muscle) Titin ANKRD1 TTN Two-hybrid Miller MK (2003) 14583192 ankyrin repeat domain 23 Titin ANKRD23 TTN Two-hybrid Miller MK (2003) 14583192 Annexin A2 Anticardiolipin ANXA2 APOH Reconstituted Complex Ma K (2000) 10809787 Annexin A2 Cathepsin B1 ANXA2 CTSB Reconstituted Complex Mai J (2000) 10777578

Supplemental Table 6 79 Defining the elastic fiber interactome

Annexin A2 Cellular ligand of annexin II ANXA2 S100A10 Affinity Capture-Western He KL (2008) 18434302 Annexin A2 plasminogen activator, tissue type ANXA2 PLAT Reconstituted Complex Roda O (2003) 12468550 Annexin V Integrin beta-5 precursor. ANXA5 ITGB5 Affinity Capture-Western Cardo-Vila M (2003) 12769841 Annexin V Squalene synthetase ANXA5 FDFT1 Two-hybrid Stelzl U (2005) 16169070 WD protein associating with integrin Annexin V cytoplasmic t ANXA5 EED Two-hybrid van Lohuizen M (1998) 9584197 antiangiogenic AK38 protein Fibronectin LPA FN1 Biochemical Activity Salonen EM (1989) 2531657 antiangiogenic AK38 protein Fibronectin LPA FN1 Reconstituted Complex Salonen EM (1989) 2531657 Anti-silencing function protein 1 homolog B [SP] Titin ASF1B TTN Affinity Capture-MS Ewing RM (2007) 17353931 Apolipoprotein D Apolipoprotein A-II precursor APOD APOA2 Co-purification Yang CY (1994) 7918467 Apolipoprotein D Apolipoprotein D APOD APOD Co-purification Yang CY (1994) 7918467 apoptosis (APO-1) antigen ligand 1 Fibronectin FASLG FN1 Reconstituted Complex Aoki K (2001) 11276204 A-Raf proto-oncogene serine/threonine- protein kina Fibulin-3 ARAF EFEMP1 Two-hybrid Yuryev A (2003) 12620389 ARID domain- containing protein 3A Tyrosine-protein kinase BTK ARID3A BTK Affinity Capture-Western Nixon JC (2004) 15203319 Arrestin beta 2 Stanniocalcin-2 ARRB2 STC2 Two-hybrid Stelzl U (2005) 16169070 Arsenite-resistance protein 2. Vimentin ARS2 VIM Two-hybrid Stelzl U (2005) 16169070 aryl hydrocarbon receptor-associated 3 Vimentin IVNS1ABP VIM Two-hybrid Stelzl U (2005) 16169070 ASC-1 complex subunit p100 Myosin-9 ASCC2 MYH9 Two-hybrid Stelzl U (2005) 16169070

Atypical -lambda/iota Serine/threonine-protein kinase SMG1 PRKCI SMG1 Affinity Capture-Western Diaz-Meco MT (1996) 8524286

Atypical protein kinase C-lambda/iota Serine/threonine-protein kinase SMG1 PRKCI SMG1 Reconstituted Complex Diaz-Meco MT (1996) 8524286

Atypical protein kinase C-lambda/iota Serine/threonine-protein kinase SMG1 PRKCI SMG1 Two-hybrid Diaz-Meco MT (1996) 8524286 B-cell linker protein Tyrosine-protein kinase BTK BLNK BTK Affinity Capture-Western Hashimoto S (1999) 10498607 Gouin-Charnet A Beta-2-microglobulin precursor Alpha-2-macroglobulin B2M A2M Reconstituted Complex (2000) 10731476 BigH3 Biglycan TGFBI BGN Invitro Reinboth B et al. 16434404 BigH3 Collagen alpha-1(I) chain TGFBI COL1A1 Invitro Hashimoto K et al. 9061001 BigH3 Collagen alpha-1(II) chain TGFBI COL2A1 Invitro Hashimoto K et al. 9061001 BigH3 Collagen alpha-1(IV) chain TGFBI COL4A1 Invitro Hashimoto K et al. 9061001 BigH3 Collagen alpha-2(I) chain TGFBI COL1A2 Invitro Billings PC 12034705 BigH3 Decorin TGFBI DCN Invitro Reinboth B et al. 16434404 biliary glycoprotein adhesion molecule Annexin A2 CEACAM1 ANXA2 Affinity Capture-MS Kirshner J (2003) 14522961 biliary glycoprotein adhesion molecule Annexin A2 CEACAM1 ANXA2 Reconstituted Complex Kirshner J (2003) 14522961 bleomycin Amyloid BLMH APP Co-fractionation Lefterov IM (2000) 10973933 bleomycin hydrolase Amyloid BLMH APP Reconstituted Complex Lefterov IM (2000) 10973933 Bone morphogenetic protein 10 Fibrillin-1 BMP10 FBN1 Invitro Sengle G et al. 18339631 Bone morphogenetic protein 10 Fibrillin-2 BMP10 FBN2 Invitro Sengle G et al. 18339631 Bone morphogenetic protein 2 Fibrillin-1 BMP2 FBN1 Invitro Sengle G et al. 18339631 Bone morphogenetic protein 2 Fibrillin-2 BMP2 FBN2 Invitro Sengle G et al. 18339631

Supplemental Table 6 80 Defining the elastic fiber interactome

Bone morphogenetic protein 4 Fibrillin-1 BMP4 FBN1 Invitro Sengle G et al. 18339631 Bone morphogenetic protein 4 Fibrillin-2 BMP4 FBN2 Invitro Sengle G et al. 18339631 Bone morphogenetic protein 7 Fibrillin-1 BMP7 FBN1 Invitro Gregory KE et al. 15929982 Bone morphogenetic protein 7 Fibrillin-2 BMP7 FBN2 Invitro Sengle G et al. 18339631

Bone sialoprotein-1 Insulin-like growth factor-binding protein 5 SPP1 IGFBP5 Reconstituted Complex Nam TJ (2000) 10698186 BR140-like protein Vimentin BRD1 VIM Two-hybrid Stelzl U (2005) 16169070 Brain protein 4.1 Vimentin SYN1 VIM Two-hybrid Stelzl U (2005) 16169070 BRCA1/BRCA2-containing complex, subunit 5 Vimentin RAD51 VIM Two-hybrid Stelzl U (2005) 16169070 breast cancer, sporadic Annexin A2 PHB ANXA2 Affinity Capture-Western Bacher S (2002) 12628297 breast cancer, sporadic Annexin A2 PHB ANXA2 Reconstituted Complex Bacher S (2002) 12628297 breast cancer, sporadic Annexin A2 PHB ANXA2 Two-hybrid Bacher S (2002) 12628297 Bruton agammaglobulinemia tyrosine kinase inhibitor Tyrosine-protein kinase BTK IBTK BTK Affinity Capture-Western Liu W (2001) 11577348 Bullous pemphigoid antigen 1 isoforms 1/2/3/4/5/8 150 kDa dynein-associated polypeptide DST DCTN1 Two-hybrid Liu JJ (2003) 14581450 Bullous pemphigoid antigen 1 isoforms 1/2/3/4/5/8 erbb2 interacting protein DST ERBB2IP Two-hybrid Favre B (2001) 11375975 Bullous pemphigoid antigen 1 isoforms 1/2/3/4/5/8 integrin beta-4 subunit DST ITGB4 Two-hybrid Hopkinson SB (2000) 10637308 Bullous pemphigoid antigen 1 isoforms 6/9/10 150 kDa dynein-associated polypeptide DST DCTN1 Two-hybrid Liu JJ (2003) 14581450 Bullous pemphigoid antigen 1 isoforms 6/9/10 erbb2 interacting protein DST ERBB2IP Two-hybrid Favre B (2001) 11375975 Bullous pemphigoid antigen 1 isoforms 6/9/10 integrin beta-4 subunit DST ITGB4 Two-hybrid Hopkinson SB (2000) 10637308

CAAT-box DNA-binding protein subunit A High mobility group protein NFYA HMGA1 Far Western Currie RA (1997) 9388234

Calcitonin receptor precursor Thrombospondin-1 CALCR THBS1 Reconstituted Complex Goicoechea S (2002) 12147682 van de Graaf SF calcium transporter 2 Annexin A2 TRPV5 ANXA2 Reconstituted Complex (2003) 12660155 Calcium-regulated heat-stable protein of 24 kDa Stanniocalcin-2 CARHSP1 STC2 Two-hybrid Stelzl U (2005) 16169070 calmodulin 1 A-kinase anchor protein 9 CALM1 AKAP9 Reconstituted Complex Takahashi M (2002) 12221128 calmodulin 2 A-kinase anchor protein 9 CALM2 AKAP9 Two-hybrid Takahashi M (2002) 12221128 Calreticulin precursor Amyloid CALR APP Affinity Capture-Western Johnson RJ () 11378243 Calsyntenin-1 Amyloid CLSTN1 APBA2 Affinity Capture-Western Araki Y (2004) 15037614 Calsyntenin-1 Amyloid CLSTN1 APP Affinity Capture-Western Araki Y (2004) 15037614 Homo sapiens (clone cc44) senilin 1 (PS1; Calsyntenin-1 S182) mR CLSTN1 PSEN1 Affinity Capture-Western Araki Y (2004) 15037614

Supplemental Table 6 81 Defining the elastic fiber interactome

Carboxypeptidase N small subunit Complement C3 CPN1 C3 Biochemical Activity Campbell WD (2002) 11939578 Cartilage matrix protein precursor Matrilin-2 MATN1 MATN2 Reconstituted Complex Frank S (2002) 11896063 casein kinase 1 epsilon A-kinase anchor protein 9 Csnk1e AKAP9 Two-hybrid Sillibourne JE (2002) 12270714

Casein kinase I isoform delta A-kinase anchor protein 9 Csnk1d AKAP9 Biochemical Activity Sillibourne JE (2002) 12270714

Casein kinase I isoform delta A-kinase anchor protein 9 Csnk1d AKAP9 Two-hybrid Sillibourne JE (2002) 12270714 Caveolin-1 Tyrosine-protein kinase BTK CAV1 BTK Affinity Capture-Western Vargas L (2002) 11751885 CD247 antigen, zeta subunit Dedicator of cytokinesis protein 2 CD3Z DOCK2 Affinity Capture-Western Nishihara H (2002) 12176041 CD36 antigen (collagen type I receptor, thrombospo Thrombospondin-1 SCARB2 THBS1 Reconstituted Complex Crombie R (1998) 9478926 Cellular ligand of annexin II Annexin A2 S100A10 ANXA2 Affinity Capture-Western He KL (2008) 18434302 Cellular ligand of annexin II Annexin A2 S100A10 ANXA2 Co-crystal Structure Rety S (1999) 9886297 Class B basic helix-loop-helix protein 2 Vimentin BHLHB2 VIM Two-hybrid Stelzl U (2005) 16169070

Coagulation factor XIII A chain Fibronectin F13A1 FN1 Reconstituted Complex Achyuthan KE (1996) 8905624 Collagen alpha-1 Amyloid COL25A1 APP Co-localization Hashimoto T (2002) 11927537 Collagen alpha-1 Collagen alpha-1(I) chain COL7A1 COL1A1 Reconstituted Complex Chen M (1997) 9169408 Collagen alpha-1 Collagen alpha-1(IV) chain COL7A1 COL4A1 Reconstituted Complex Chen M (1997) 9169408 Collagen alpha-1 Fibronectin COL7A1 FN1 Reconstituted Complex Chen M (1997) 9169408

Collagen alpha-1(I) chain 72 kDa gelatinase COL1A1 MMP2 Reconstituted Complex Ellerbroek SM (2001) 11368514 Collagen alpha-1(I) chain Collagen alpha-2(I) chain COL1A1 COL1A2 Miles C A et al. 12206762 Collagen alpha-1(I) chain Insulin-like growth factor 3 COL1A1 IGFBP3 Affinity Capture-Western Liu B () 12735930

Collagen alpha-1(I) chain Insulin-like growth factor-binding protein 3 COL1A1 IGFBP3 Affinity Capture-Western Liu B () 12735930 Collagen alpha-1(I) chain integrin VLA-4 beta subunit COL1A1 ITGB1 Co-purification Staatz WD (1990) 2156854 Collagen alpha-1(I) chain nidogen (enactin) COL1A1 Nid1 Reconstituted Complex Kohfeldt E (1998) 9733643 Collagen alpha-1(I) chain Nidogen-1 precursor COL1A1 NID Reconstituted Complex Kohfeldt E (1998) 9733643 Collagen alpha-1(I) chain Nidogen-2 precursor COL1A1 NID2 Reconstituted Complex Kohfeldt E (1998) 9733643 Osteonectin (secreted protein, acidic, Collagen alpha-1(I) chain cysteine-ri COL1A1 SPARC Reconstituted Complex Sage H (1989) 2745554 Secreted protein acidic and rich in cysteine Collagen alpha-1(I) chain |Oste COL1A1 SPARC Reconstituted Complex Sage H (1989) 2745554 Collagen alpha-1(I) chain VLA-2 alpha chain COL1A1 ITGA2 Co-purification Staatz WD (1990) 2156854 Collagen alpha-1(II) chain Annexin V COL2A1 ANXA5 Reconstituted Complex Kirsch T (1992) 1397263 Collagen alpha-1(IV) chain Collagen alpha-1(IV) chain COL4A1 COL4A1 Co-crystal Structure Than ME (2002) 12011424 Collagen alpha-1(IV) chain nidogen (enactin) COL4A1 Nid1 Reconstituted Complex Kohfeldt E (1998) 9733643 Collagen alpha-1(IV) chain Nidogen-1 precursor COL4A1 NID Reconstituted Complex Kohfeldt E (1998) 9733643 Collagen alpha-1(IV) chain Nidogen-2 precursor COL4A1 NID2 Reconstituted Complex Kohfeldt E (1998) 9733643 Collagen alpha-1(VII) chain Collagen alpha-1(I) chain COL7A1 COL1A1 Reconstituted Complex Chen M (1997) 9169408

Supplemental Table 6 82 Defining the elastic fiber interactome

Collagen alpha-1(VII) chain Fibronectin COL7A1 FN1 Reconstituted Complex Chen M (1997) 9169408 Collagen alpha-1(VIII) chain Collagen alpha-2(VIII) chain COL8A1 COL8A2 Reconstituted Complex Illidge C (1998) 9705353 Collagen alpha-1(VIII) chain collagen VIII, alpha-2 polypeptide COL8A1 COL8A2 Reconstituted Complex Illidge C (1998) 9705353 Collagen alpha-1(VIII) chain kelch-like protein C3IP1 COL8A1 KLHL12 Two-hybrid Rual JF (2005) 16189514 Ultrahigh sulfur keratin-associated protein Collagen alpha-1(VIII) chain 4.12 COL8A1 KRTAP4-12 Two-hybrid Rual JF (2005) 16189514 Collagen alpha-1(VIII) chain Weakly similar to extracellular protein COL8A1 EFEMP2 Two-hybrid Rual JF (2005) 16189514 Vascular endothelial growth factor receptor Collagen alpha-1(XVIII) chain 2 COL18A1 KDR Reconstituted Complex Kim YM (2002) 12029087 Collagen alpha-2(IV) chain Collagen alpha-1(IV) chain COL4A2 COL4A1 Co-crystal Structure Than ME (2002) 12011424 Collagen alpha-2(IV) chain Collagen alpha-2(IV) chain COL4A2 COL4A2 Co-crystal Structure Than ME (2002) 12011424 collagen, type X, alpha 1 Annexin V COL10A1 ANXA5 Reconstituted Complex Kirsch T (1992) 1397263 Collagen/fibrinogen domain-containing protein 1 Elastin FCN1 ELN Reconstituted Complex Harumiya S (1996) 8947836 Complement C1q Complement C1q C1QTNF5 C1QTNF5 Reconstituted Complex Hayward C (2003) 12944416

Complement C1q LARGE PROLINE-RICH PROTEIN BAT2 C1QBP BAT2 Two-hybrid Lehner B (2004) 14667819 Complement C1q Protein kinase C alpha type C1QBP PRKCA Affinity Capture-Western Storz P (2000) 10831594 Complement C1q Protein kinase C alpha type C1QBP PRKCA Reconstituted Complex Storz P (2000) 10831594 Complement C1q protein kinase C, delta C1QBP PRKCD Affinity Capture-Western Storz P (2000) 10831594 Complement C1q protein kinase C, delta C1QBP PRKCD Reconstituted Complex Storz P (2000) 10831594 Complement C1q protein kinase C, mu C1QBP PRKD1 Affinity Capture-Western Storz P (2000) 10831594 Complement C1q protein kinase C, mu C1QBP PRKD1 Reconstituted Complex Storz P (2000) 10831594 Complement C1q protein kinase C, zeta C1QBP PRKCZ Affinity Capture-Western Storz P (2000) 10831594 Complement C1q protein kinase C, zeta C1QBP PRKCZ Reconstituted Complex Storz P (2000) 10831594 Complement C1q Protein kinase C-alpha-binding protein C1QBP PRKCA Affinity Capture-Western Storz P (2000) 10831594 Complement C1q Protein kinase C-alpha-binding protein C1QBP PRKCA Reconstituted Complex Storz P (2000) 10831594 splicing factor, arginine/serine-rich 1 Petersen-Mahrt SK Complement C1q (splicing C1QBP SFRS1 Reconstituted Complex (1999) 10022843 Petersen-Mahrt SK Complement C1q Splicing factor, arginine/serine-rich 9 C1QBP SFRS9 Far Western (1999) 10022843 Complement C3 Complement C5 precursor C3 C5 Reconstituted Complex Jokiranta TS (2001) 11367533 Complement C3 Complement factor H C3 CFH Reconstituted Complex Jokiranta TS (2001) 11367533 Complement C3 factor H-related protein 5 C3 CFHL5 Reconstituted Complex McRae JL (2001) 11058592 Complement C3 Glycine-rich beta glycoprotein C3 BF Reconstituted Complex Jokiranta TS (2001) 11367533 Complement C3 H factor 1 (complement) C3 CFH Reconstituted Complex Jokiranta TS (2001) 11367533 integrin, alpha X (antigen CD11C (p150), Complement C3 alpha pol C3 ITGAX Reconstituted Complex Micklem KJ (1985) 4062888 Complement C3 Properdin precursor C3 PFC Reconstituted Complex Farries TC (1988) 3421908

Complement C3 Properdin precursor C3 PFC Reconstituted Complex Perdikoulis MV (2001) 11513971 complement component C1q, A chain Fibronectin C1QA FN1 Reconstituted Complex Bing DH (1982) 6981115

Supplemental Table 6 83 Defining the elastic fiber interactome

Complement factor H-related protein 3 precursor Complement C3 CFHL3 C3 Reconstituted Complex Hellwage J (1999) 10622723 Complement factor H-related protein 4 precursor Complement C3 CFHL4 C3 Reconstituted Complex Hellwage J (1999) 10622723 complement S-protein Insulin-like growth factor II VTN IGF2 Reconstituted Complex Upton Z (1999) 10342887 Mulligan-Kehoe MJ complement S-protein Plasminogen activator inhibitor 1 VTN SERPINE1 Reconstituted Complex (2001) 11113116 complement S-protein TGF beta-2 VTN TGFB2 Reconstituted Complex Schoppet M (2002) 11796824 covering first half of fourth kringle Insulin-like growth factor-binding protein 3 PLG IGFBP3 Co-purification Campbell PG (1998) 9688635 covering first half of fourth kringle Insulin-like growth factor-binding protein 3 PLG IGFBP3 Reconstituted Complex Campbell PG (1998) 9688635 Mulligan-Kehoe MJ covering first half of fourth kringle Plasminogen activator inhibitor 1 PLG SERPINE1 Reconstituted Complex (2001) 11113116 covering first half of fourth kringle Thrombospondin-1 PLG THBS1 Reconstituted Complex DePoli P (1989) 2522013 cystatin A (stefin A) Cathepsin H CSTA CTSH Co-crystal Structure Jenko S (2003) 12581647 cystatin B (liver thiol proteinase inhibitor) Cathepsin H CSTB CTSH Reconstituted Complex Pol E (2001) 11514663 cysteine protease Vimentin CASP8 VIM Two-hybrid Stelzl U (2005) 16169070 Dedicator of cytokinesis protein 2 Proto-oncogene vav. DOCK2 VAV1 Affinity Capture-Western Nishihara H (2002) 12393632 v-crk sarcoma virus CT10 oncogene Dedicator of cytokinesis protein 2 homolog (avian)- DOCK2 CRKL Affinity Capture-Western Nishihara H (2002) 12393632 Dehydrodolichyl diphosphate synthase Lysyl oxidase DHDDS LOX Two-hybrid Kharel Y (2004) 15110773 delta helicase Serine/threonine-protein kinase SMG1 RENT1 SMG1 Affinity Capture-Western Yamashita A (2001) 11544179 dentatorubral-pallidoluysian atrophy (atrophin-1) Vimentin ATN1 VIM Two-hybrid Stelzl U (2005) 16169070 Vimentin DSP VIM Reconstituted Complex Meng JJ (1997) 9261168 Desmoplakin Vimentin DSP VIM Two-hybrid Meng JJ (1997) 9261168 Developmental pluripotency-associated protein 4. Vimentin DPPA4 VIM Two-hybrid Stelzl U (2005) 16169070 Dickkopf-related protein 1 actin-binding protein DKK1 CORO1A Affinity Capture-MS Ewing RM (2007) 17353931 Dickkopf-related protein 1 low density lipoprotein-related protein 6 DKK1 LRP6 Affinity Capture-Western Semenov MV (2001) 11448771 dihydrofolate reductase Thrombospondin-1 DHFR THBS1 Reconstituted Complex Vanguri VK (2000) 10749676 disabled (Drosophila) homolog 2 (mitogen- responsive phosphoprotein) Amyloid DAB2 APP Reconstituted Complex Morris SM (2001) 11247302 Disrupted in schizophrenia 1 protein. Collagen alpha-1(IV) chain DISC1 COL4A1 Two-hybrid Morris JA (2003) 12812986 DNA-directed RNA polymerase II 33 kDa polypeptide Stanniocalcin-2 POLR2C STC2 Two-hybrid Stelzl U (2005) 16169070 dominant-negative kinase-deficient Brutons tyrosin Tyrosine-protein kinase BTK BTK BTK Co-crystal Structure Mao C (2001) 11527964 Elastin dermatan sulphate proteoglycan I ELN BGN Reconstituted Complex Reinboth B (2002) 11723132 Elastin dermatan sulphate proteoglycans II ELN DCN Reconstituted Complex Reinboth B (2002) 11723132

Supplemental Table 6 84 Defining the elastic fiber interactome

Elastin Fibrillin-2 ELN FBN2 Reconstituted Complex Trask TM (2000) 10825173 Elastin fibulin 2 ELN FBLN2 Reconstituted Complex Sasaki T (1999) 10544250 Elastin Fibulin-1 ELN FBLN1 Reconstituted Complex Sasaki T (1999) 10544250 Elastin Nidogen-2 precursor ELN NID2 Reconstituted Complex Sasaki T (1999) 10544250 elongin binding protein Fibronectin VHL FN1 Affinity Capture-Western Ohh M (1998) 9651579 emerin (Emery-Dreifuss muscular dystrophy) Lamin-A/C EMD LMNA Affinity Capture-Western Lattanzi G (2003) 12670476 emerin (Emery-Dreifuss muscular dystrophy) Lamin-A/C EMD LMNA Affinity Capture-Western Wilkinson FL (2003) 12755701 emerin (Emery-Dreifuss muscular dystrophy) Lamin-A/C EMD LMNA Two-hybrid Sakaki M (2001) 11173535 emerin (Emery-Dreifuss muscular dystrophy) Lamin-A/C EMD LMNA Two-hybrid Wilkinson FL (2003) 12755701 endocrine adenomatosis, multiple Vimentin MEN1 VIM Affinity Capture-Western Lopez-Egido J (2002) 12169273 endocrine adenomatosis, multiple Vimentin MEN1 VIM Reconstituted Complex Lopez-Egido J (2002) 12169273 Bullous pemphigoid antigen 1 isoforms erbb2 interacting protein 1/2/3/4/5/8 ERBB2IP DST Reconstituted Complex Favre B (2001) 11375975 Bullous pemphigoid antigen 1 isoforms erbb2 interacting protein 6/9/10 ERBB2IP DST Reconstituted Complex Favre B (2001) 11375975 eukaryotic initiation factor 2B, subunit 3 (gamma, 58kD) Stanniocalcin-2 EIF2B3 STC2 Two-hybrid Stelzl U (2005) 16169070 Ewing sarcoma breakpoint region 1 High mobility group protein EWSR1 HMGA1 Two-hybrid Rual JF (2005) 16189514 EWS oncogene High mobility group protein EWSR1 HMGA1 Two-hybrid Rual JF (2005) 16189514 Extracellular glycoprotein lacritin precursor. Fibronectin LACRT FN1 Reconstituted Complex Sanghi S (2001) 11419941 fatty acid binding protein 4, adipocyte Vimentin FABP4 VIM Two-hybrid Stelzl U (2005) 16169070 FGF-binding protein 1 Perlecan FGFBP1 HSPG2 Affinity Capture-Western Mongiat M (2001) 11148217 FGF-binding protein 1 Perlecan FGFBP1 HSPG2 Two-hybrid Mongiat M (2001) 11148217 Fibrillin-1 Elastin FBN1 ELN Invitro Rock MJ et al. 15039439 Fibrillin-1 Fibrillin-1 FBN1 FBN1 Invitro Lin G et al. 12399449 Fibrillin-1 Fibrillin-1 FBN1 FBN1 Invitro Marson A et al. 15569675

Fibrillin-1 Fibrillin-2 FBN1 FBN2 Invitro Charbonneau NL et al. 12429739 Fibrillin-1 Fibrillin-2 FBN1 FBN2 Invitro Lin G et al. 12399449 Fibrillin-1 Fibronectin FBN1 FN1 Invitro Kinsey R et al. 18653538 Fibrillin-1 Fibronectin FBN1 FN1 Invitro Sabatier L et al. 19037100 Fibrillin-1 Fibulin-2 FBN1 FBLN2 Invitro El-Hallous E, et al. 17255108 Fibrillin-1 Fibulin-4 FBN1 EFEMP2 Invitro El-Hallous E, et al. 17255108 Fibrillin-1 Fibulin-5 FBN1 FBLN5 Invitro El-Hallous E, et al. 17255108 Fibrillin-1 LTBP-1 FBN1 LTBP1 Invitro Isogai Z et al. 12429738

Supplemental Table 6 85 Defining the elastic fiber interactome

Fibrillin-1 MAGP-1 FBN1 MFAP2 Invitro Rock MJ et al. 15039439 Fibrillin-1 MAGP-1 FBN1 MFAP2 Reconstituted Complex Jensen SA (2001) 11481325 Fibronectin BigH3 FN1 TGFBI Invitro Billings PC 12034705 Fibronectin BigH3 FN1 TGFBI Reconstituted Complex Kim JE (2002) 11867580 Fibronectin Collagen alpha-1 FN1 COL7A1 Reconstituted Complex Lapiere JC (1994) 7963647 Fibronectin Collagen alpha-1(I) chain FN1 COL1A1 Invitro Steffensen B et al. 12225805 Fibronectin Collagen alpha-1(II) chain FN1 COL2A1 Invitro Steffensen B et al. 12225805 Fibronectin Collagen alpha-1(III) chain FN1 COL3A1 Invitro Steffensen B et al. 12225805 Fibronectin Collagen alpha-1(IV) chain FN1 COL4A1 Invitro Steffensen B et al. 12225805 Fibronectin Collagen alpha-1(V) chain FN1 COL5A1 Invitro Steffensen B et al. 12225805 Fibronectin Collagen alpha-1(VII) chain FN1 COL7A1 Reconstituted Complex Lapiere JC (1994) 7963647 Fibronectin Collagen alpha-1(X) chain FN1 COL10A1 Invitro Steffensen B et al. 12225805 Fibronectin Collagen alpha-2(I) chain FN1 COL1A2 Invitro Steffensen B et al. 12225805 Fibronectin covering first half of fourth kringle FN1 PLG Reconstituted Complex Moser TL (1993) 8360181 Fibronectin C-reactive protein precursor FN1 CRP Reconstituted Complex Salonen EM (1984) 6693419 Fibronectin fibronectin receptor, alpha subunit FN1 ITGA5 Reconstituted Complex Busk M (1992) 1532572 Fibronectin fibulin 2 FN1 FBLN2 Reconstituted Complex Sasaki T (1995) 7500359 Fibronectin Fibulin-1 FN1 FBLN1 Reconstituted Complex Balbona K (1992) 1400330 Fibronectin integrin beta 7 subunit FN1 ITGB7 Affinity Capture-Western Ruegg C (1992) 1372909 integrin, alpha 5 (fibronectin receptor, Fibronectin alpha pol FN1 ITGA5 Reconstituted Complex Busk M (1992) 1532572 Fibronectin integrin, beta 6 FN1 ITGB6 Reconstituted Complex Busk M (1992) 1532572 Melanoma-derived growth regulatory Fibronectin protein precurs FN1 MIA Reconstituted Complex Stoll R (2001) 11157741 Fibronectin plasminogen activator, tissue type FN1 PLAT Reconstituted Complex Moser TL (1993) 8360181 Fibronectin Serum amyloid P-component precursor FN1 APCS Affinity Capture-Western Sen JW (2002) 12100475 Fibronectin Stromal cell-derived factor 1 precursor FN1 CXCL12 Reconstituted Complex Pelletier AJ (2000) 11023498 Fibronectin Tenascin FN1 TNC Affinity Capture-Western Chung CY (1995) 7499434 Fibronectin Tenascin FN1 TNC Reconstituted Complex Chung CY (1995) 7499434 Fibronectin Thrombospondin-1 FN1 THBS1 Invitro Zafar R S et al. 1497347 Fibronectin thyrotropin receptor FN1 TSHR Reconstituted Complex Ghinea N (2002) 11981027 Fibronectin Tribbles homolog 3 FN1 TRIB3 Affinity Capture-Western Zhou Y (2008) 18276110 fibulin 2 Fibrillin-1 FBLN2 FBN1 Reconstituted Complex Reinhardt DP (1996) 8702639 Fibulin-1 Amyloid FBLN1 APP Reconstituted Complex Ohsawa I (2001) 11238726 Fibulin-1 Connective tissue growth factor FBLN1 CTGF Reconstituted Complex Perbal B (1999) 9927660 Fibulin-1 Fibrinogen beta chain FBLN1 FGB Reconstituted Complex Tran H (1995) 7642629 Fibulin-1 Fibulin-1 FBLN1 FBLN1 Reconstituted Complex Tran H (1997) 9278415 Fibulin-1 Nidogen-1 precursor FBLN1 NID Reconstituted Complex Adam S (1997) 9299350 Fibulin-1 Nidogen-1 precursor FBLN1 NID Reconstituted Complex Tran H (1997) 9278415 Fibulin-1 Protein NOV homolog FBLN1 NOV Reconstituted Complex Perbal B (1999) 9927660 A-Raf proto-oncogene serine/threonine-

Supplemental Table 6 86 Defining the elastic fiber interactome

Small glutamine-rich tetratricopeptide Fibulin-3 repeat-cont EFEMP1 SGTA Two-hybrid Rual JF (2005) 16189514 Fibulin-4 Fibrillin-1 EFEMP2 FBN1 Invitro Choudhury R et al. 19570982 Fibulin-4 Lysyl oxidase EFEMP2 LOX Invitro Choudhury R et al. 19570982 Fibulin-5 Elastin FBLN5 ELN Invitro Choudhury R et al. 19570982 Fibulin-5 Fibrillin-1 FBLN5 FBN1 Invitro Choudhury R et al. 19570982 FK506-binding protein, T-cell, 12-kD Ryanodine receptor 3 FKBP1A RYR3 Reconstituted Complex Bultynck G (2001) 11171121 formin-binding protein 17 A-kinase anchor protein 9 FNBP1 AKAP9 Two-hybrid Larocca MC (2004) 15047863 Four and a half LIM domains protein 2 Titin FHL2 TTN Reconstituted Complex Lange S (2002) 12432079 Four and a half LIM domains protein 2 Titin FHL2 TTN Two-hybrid Lange S (2002) 12432079 gastric intrinsic factor (vitamin B synthesis) Complement C3 IF C3 Biochemical Activity Lambris JD (1996) 8648130 gastric intrinsic factor (vitamin B synthesis) Complement C3 IF C3 Reconstituted Complex Soames CJ (1997) 9291131 (amyloidosis, Finnish type) Amyloid GSN APP Affinity Capture-Western Chauhan VP (1999) 10329371 gelsolin (amyloidosis, Finnish type) Amyloid GSN APP Reconstituted Complex Chauhan VP (1999) 10329371 gelsolin (amyloidosis, Finnish type) Fibronectin GSN FN1 Reconstituted Complex Lind SE (1984) 6092370 General transcription factor II-I Tyrosine-protein kinase BTK GTF2I BTK Affinity Capture-Western Novina CD (1999) 10373551 General transcription factor II-I Tyrosine-protein kinase BTK GTF2I BTK Affinity Capture-Western Yang W (1997) 9012831 Glial fibrillary acidic protein Vimentin GFAP VIM Two-hybrid Rual JF (2005) 16189514

RGD130509 Golgin subfamily B member 1 GOLGB1 0 predicted Two-hybrid Stelzl U (2005) 16169070 Golgin subfamily B member 1 breast cancer metastasis suppressor 1 GOLGB1 BRMS1 Two-hybrid Stelzl U (2005) 16169070 Golgin subfamily B member 1 hypothetical protein LOC57562 GOLGB1 KIAA1377 Two-hybrid Stelzl U (2005) 16169070 Peripheral benzodiazepine receptor- Golgin subfamily B member 1 associated prot GOLGB1 ACBD3 Far Western Sohda M (2001) 11590181 Peripheral benzodiazepine receptor- Golgin subfamily B member 1 associated prot GOLGB1 ACBD3 Two-hybrid Sohda M (2001) 11590181 Golgin subfamily B member 1 2 GOLGB1 PFN2 Two-hybrid Stelzl U (2005) 16169070 Dirac-Svejstrup AB Golgin subfamily B member 1 Vesicle docking protein GOLGB1 VDP Reconstituted Complex (2000) 10931861 GRB2-associated binder 1 Complement C1q GAB1 C1QBP Affinity Capture-Western Braun L (2000) 10747014 Growth differentiation factor 5 Fibrillin-1 GDF5 FBN1 Invitro Sengle G et al. 18339631 Growth differentiation factor 5 Fibrillin-2 GDF5 FBN2 Invitro Sengle G et al. 18339631 Growth/differentiation factor 8 precursor Follistatin-related protein GDF8 FSTL3 Affinity Capture-MS Hill JJ (2002) 12194980 Growth/differentiation factor 8 precursor Follistatin-related protein GDF8 FSTL3 Affinity Capture-Western Hill JJ (2002) 12194980 Growth/differentiation factor 8 precursor Follistatin-related protein GDF8 FSTL3 Reconstituted Complex Hill JJ (2002) 12194980 Heterogeneous nuclear ribonucleoprotein GST class-kappa [SP] M GSTK1 HRNR Affinity Capture-MS Ewing RM (2007) 17353931 H factor 1 (complement) Complement C3 CFH C3 Reconstituted Complex Soames CJ (1997) 9291131

Supplemental Table 6 87 Defining the elastic fiber interactome

heparan sulfate proteoglycan Fibronectin CD44 FN1 Affinity Capture-Western Jalkanen S (1992) 1730778 heparan sulfate proteoglycan Fibronectin CD44 FN1 Reconstituted Complex Jalkanen S (1992) 1730778 heparan sulfate proteoglycan core protein |Heparan Fibronectin SDC2 FN1 Reconstituted Complex Kusano Y (2000) 10772816 hepatocellular carcinoma-related putative Heterogeneous nuclear ribonucleoprotein tumor suppressor [EG] M PINX1 HRNR Affinity Capture-MS Ewing RM (2007) 17353931 Hepatocyte growth factor-regulated tyrosine kinase Amyloid HGS APP Reconstituted Complex Pridgeon JW (2009) 19019082 High mobility group protein Citrulline--aspartate HMGA1 ATF2 Reconstituted Complex Yie J (1999) 10357819 High mobility group protein E74-like factor 1 HMGA1 ELF1 Reconstituted Complex John S (1995) 7862168 High mobility group protein interferon regulatory factor 1 HMGA1 IRF1 Reconstituted Complex Yie J (1999) 10357819 High mobility group protein Nuclear factor NF- IL6 HMGA1 CEBPB Reconstituted Complex Foti D (2003) 12665574 High mobility group protein Sp1 transcription factor HMGA1 SP1 Affinity Capture-Western Foti D (2003) 12665574 High mobility group protein Sp1 transcription factor HMGA1 SP1 Reconstituted Complex Foti D (2003) 12665574 v-jun sarcoma virus 17 oncogene homolog High mobility group protein (avian) HMGA1 JUN Reconstituted Complex Yie J (1999) 10357819 high-mobility group 20B Vimentin HMG20B VIM Two-hybrid Stelzl U (2005) 16169070

HK III Insulin-like growth factor-binding protein 4 HK3 IGFBP4 Reconstituted Complex Sui D (2000) 11068878

HK III Insulin-like growth factor-binding protein 4 HK3 IGFBP4 Two-hybrid Sui D (2000) 11068878

Hornerin Histone H2B HSPD1 HIST2H2BE Affinity Capture-Western Khan IU (1998) 9724719 hypothetical protein LOC55701 Stanniocalcin-2 FLJ10357 STC2 Two-hybrid Stelzl U (2005) 16169070 IgE-binding protein Elastin LGALS3 ELN Affinity Capture-Western Ochieng J (1999) 10536372 IgE-binding protein Elastin LGALS3 ELN Reconstituted Complex Ochieng J (1999) 10536372 Immunoglobulin heavy chain-binding protein Alpha-2-macroglobulin HSPA5 A2M Reconstituted Complex Misra UK (2002) 12194978 Inhibitor of nuclear factor kappa-B kinase subunit epsilon [SP] Protein disulfide isomerase A6 IKBKE PDIA6 Affinity Capture-MS Ewing RM (2007) 17353931 Insulin protease Insulin-like growth factor II IDE IGF2 Reconstituted Complex Ding L (1992) 1733942 Insulin-like growth factor 3 Collagen alpha-1(I) chain IGFBP3 COL1A1 Co-purification Liu B () 12735930 Insulin-like growth factor 3 Collagen alpha-1(I) chain IGFBP3 COL1A1 Two-hybrid Liu B () 12735930 Insulin-like growth factor 3 Fibronectin IGFBP3 FN1 Affinity Capture-Western Gui Y (2001) 11344214 Insulin-like growth factor 3 Fibronectin IGFBP3 FN1 Reconstituted Complex Gui Y (2001) 11344214 Insulin-like growth factor 3 Fibronectin IGFBP3 FN1 Reconstituted Complex Martin JA (2002) 12127836 Insulin-like growth factor 3 Fibronectin IGFBP3 FN1 Two-hybrid Gui Y (2001) 11344214 Insulin-like growth factor 3 Insulin-like growth factor II IGFBP3 IGF2 Reconstituted Complex Buckway CK (2001) 11600567 Insulin-like growth factor 3 Insulin-like growth factor II IGFBP3 IGF2 Reconstituted Complex Firth SM (1998) 9446566 Insulin-like growth factor 3 Insulin-like growth factor II IGFBP3 Igf2 Two-hybrid Gui Y (2003) 12962157 Insulin-like growth factor 4 Insulin-like growth factor II IGFBP4 IGF2 Reconstituted Complex Qin X (1998) 9722589

Supplemental Table 6 88 Defining the elastic fiber interactome

Insulin-like growth factor IB precursor Insulin-like growth factor-binding protein 3 IGF1 IGFBP3 Reconstituted Complex Liu B () 12735930

Insulin-like growth factor IB precursor Insulin-like growth factor-binding protein 3 IGF1 IGFBP3 Reconstituted Complex Twigg SM (1998) 9497324

Insulin-like growth factor IB precursor Insulin-like growth factor-binding protein 4 IGF1 IGFBP4 Reconstituted Complex Bach LA (1993) 7683646

Insulin-like growth factor IB precursor Insulin-like growth factor-binding protein 5 IGF1 IGFBP5 Reconstituted Complex Twigg SM (1998) 9497324

Insulin-like growth factor IB precursor Insulin-like growth factor-binding protein 7 IGF1 IGFBP7 Reconstituted Complex Ahmed S (2003) 14521955 Insulin-like growth factor II IGF-binding protein 6 IGF2 IGFBP6 Reconstituted Complex Bach LA (1993) 7683646 Insulin-like growth factor II Insulin-like growth factor 3 IGF2 IGFBP3 Reconstituted Complex Storch S (2001) 11749962 Insulin-like growth factor II Insulin-like growth factor 3 IGF2 IGFBP3 Reconstituted Complex Twigg SM (1998) 9497324 Insulin-like growth factor II Insulin-like growth factor 5 IGF2 IGFBP5 Reconstituted Complex Twigg SM (1998) 9497324 Insulin-like growth factor-binding protein 1 Insulin-like growth factor II precu IGF2 IGFBP1 Reconstituted Complex Bond JJ (2000) 10810289

Insulin-like growth factor II Insulin-like growth factor-binding protein 3 IGF2 IGFBP3 Reconstituted Complex Storch S (2001) 11749962

Insulin-like growth factor II Insulin-like growth factor-binding protein 3 IGF2 IGFBP3 Reconstituted Complex Twigg SM (1998) 9497324

Insulin-like growth factor II Insulin-like growth factor-binding protein 5 IGF2 IGFBP5 Reconstituted Complex Twigg SM (1998) 9497324 Insulin-like growth factor II Serotransferrin precursor IGF2 TF Reconstituted Complex Storch S (2001) 11749962

Insulin-like growth factor-binding protein 3 ADAM 12 precursor IGFBP3 ADAM12 Affinity Capture-Western Shi Z (2000) 10849447

Insulin-like growth factor-binding protein 3 ADAM 12 precursor IGFBP3 ADAM12 Two-hybrid Shi Z (2000) 10849447

Insulin-like growth factor-binding protein 3 Collagen alpha-1(I) chain IGFBP3 COL1A1 Co-purification Liu B () 12735930

Insulin-like growth factor-binding protein 3 Collagen alpha-1(I) chain IGFBP3 COL1A1 Two-hybrid Liu B () 12735930

Insulin-like growth factor-binding protein 3 Fibronectin IGFBP3 FN1 Affinity Capture-Western Gui Y (2001) 11344214

Insulin-like growth factor-binding protein 3 Fibronectin IGFBP3 FN1 Reconstituted Complex Gui Y (2001) 11344214

Insulin-like growth factor-binding protein 3 Fibronectin IGFBP3 FN1 Reconstituted Complex Martin JA (2002) 12127836

Insulin-like growth factor-binding protein 3 Fibronectin IGFBP3 FN1 Two-hybrid Gui Y (2001) 11344214

Insulin-like growth factor-binding protein 3 heparan sulfate proteoglycan IGFBP3 CD44 Reconstituted Complex Martin JA (2002) 12127836

Supplemental Table 6 89 Defining the elastic fiber interactome

Insulin-like growth factor-binding protein 3 Insulin-like growth factor IB precursor IGFBP3 IGF1 Affinity Capture-Western Buckway CK (2001) 11600567

Insulin-like growth factor-binding protein 3 Insulin-like growth factor IB precursor IGFBP3 IGF1 Affinity Capture-Western Cohen P (1992) 1383255

Insulin-like growth factor-binding protein 3 Insulin-like growth factor IB precursor IGFBP3 IGF1 Reconstituted Complex Buckway CK (2001) 11600567

Insulin-like growth factor-binding protein 3 Insulin-like growth factor IB precursor IGFBP3 IGF1 Reconstituted Complex Firth SM (1998) 9446566

Insulin-like growth factor-binding protein 3 Insulin-like growth factor II IGFBP3 IGF2 Reconstituted Complex Buckway CK (2001) 11600567

Insulin-like growth factor-binding protein 3 Insulin-like growth factor II IGFBP3 IGF2 Reconstituted Complex Firth SM (1998) 9446566

Insulin-like growth factor-binding protein 3 Insulin-like growth factor II IGFBP3 Igf2 Two-hybrid Gui Y (2003) 12962157 Latent-transforming growth factor beta- Insulin-like growth factor-binding protein 3 binding pro IGFBP3 Ltbp1 Two-hybrid Gui Y (2003) 12962157 polymerase (DNA directed), alpha 2 (70kD Insulin-like growth factor-binding protein 3 subunit) IGFBP3 POLA2 Two-hybrid Stelzl U (2005) 16169070

Insulin-like growth factor-binding protein 3 Serotransferrin precursor IGFBP3 TF Affinity Capture-Western Weinzimer SA (2001) 11297622

Insulin-like growth factor-binding protein 3 Serotransferrin precursor IGFBP3 TF Reconstituted Complex Weinzimer SA (2001) 11297622

Insulin-like growth factor-binding protein 3 TFIIIC 220 kDa subunit IGFBP3 GTF3C1 Two-hybrid Stelzl U (2005) 16169070

Insulin-like growth factor-binding protein 4 Insulin-like growth factor IB precursor IGFBP4 IGF1 Reconstituted Complex Qin X (1998) 9722589

Insulin-like growth factor-binding protein 4 Insulin-like growth factor II IGFBP4 IGF2 Reconstituted Complex Qin X (1998) 9722589

Insulin-like growth factor-binding protein 5 complement S-protein IGFBP5 VTN Reconstituted Complex Nam T (2002) 11751588

Insulin-like growth factor-binding protein 5 Four and a half LIM domains protein 2 IGFBP5 FHL2 Affinity Capture-Western Amaar YG (2002) 11821401

Insulin-like growth factor-binding protein 5 Four and a half LIM domains protein 2 IGFBP5 FHL2 Reconstituted Complex Amaar YG (2002) 11821401

Insulin-like growth factor-binding protein 5 Four and a half LIM domains protein 2 IGFBP5 FHL2 Two-hybrid Amaar YG (2002) 11821401

Insulin-like growth factor-binding protein 5 Protein G3 IGFBP5 BAT3 Two-hybrid Stelzl U (2005) 16169070

Insulin-like growth factor-binding protein 7 Collagen alpha-1(II) chain Igfbp7 COL2A1 Affinity Capture-Western Nagakubo D (2003) 12847218

Supplemental Table 6 90 Defining the elastic fiber interactome

Insulin-like growth factor-binding protein 7 Collagen alpha-1(V) chain Igfbp7 COL5A1 Affinity Capture-Western Nagakubo D (2003) 12847218

Insulin-like growth factor-binding protein 7 gamma IP10 Igfbp7 CXCL10 Reconstituted Complex Nagakubo D (2003) 12847218

Insulin-like growth factor-binding protein 7 Insulin precursor IGFBP7 INS Reconstituted Complex Yamanaka Y (1997) 9388210

Insulin-like growth factor-binding protein 7 Insulin-like growth factor IB precursor IGFBP7 IGF1 Reconstituted Complex Oh Y (1996) 8939990

Insulin-like growth factor-binding protein 7 Insulin-like growth factor II IGFBP7 IGF2 Reconstituted Complex Oh Y (1996) 8939990

Insulin-like growth factor-binding protein 7 Neuroendocrine differentiation factor IGFBP7 VPS24 Affinity Capture-Western Wilson EM (2001) 11549700

Insulin-like growth factor-binding protein 7 Neuroendocrine differentiation factor IGFBP7 VPS24 Two-hybrid Wilson EM (2001) 11549700

Insulin-like growth factor-binding protein 7 Secondary lymphoid-tissue chemokine Igfbp7 CCL21 Reconstituted Complex Nagakubo D (2003) 12847218 small inducible cytokine subfamily A (Cys- Insulin-like growth factor-binding protein 7 Cys), me Igfbp7 CCL5 Reconstituted Complex Nagakubo D (2003) 12847218 Vascular endothelial growth factor A Insulin-like growth factor-binding protein 7 precursor IGFBP7 VEGF Reconstituted Complex Usui T (2002) 12407018 Insulin-like growth factor-binding protein complex Insulin-like growth factor-binding protein 3 IGFALS IGFBP3 Reconstituted Complex Firth SM (1998) 9446566 Insulin-like growth factor-binding protein complex Insulin-like growth factor-binding protein 3 IGFALS IGFBP3 Reconstituted Complex Twigg SM (1998) 9497324 Insulin-like growth factor-binding protein complex Insulin-like growth factor-binding protein 5 IGFALS IGFBP5 Reconstituted Complex Twigg SM (1998) 9497324 Grzeszkiewicz TM integrin alpha-V Protein CYR61 ITGAV CYR61 Reconstituted Complex (2001) 11287419 Integrin beta-5 precursor. Annexin V ITGB5 ANXA5 Reconstituted Complex Cardo-Vila M (2003) 12769841 Grzeszkiewicz TM Integrin beta-5 precursor. Protein CYR61 ITGB5 CYR61 Reconstituted Complex (2001) 11287419 intracellular antigen detected by monoclonal antib Vimentin HABP4 VIM Two-hybrid Stelzl U (2005) 16169070 Takahashi-Tezuka M janus kinase 1 Tyrosine-protein kinase BTK JAK1 BTK Affinity Capture-Western (1997) 9178903 Kallikrein-like protein 4 Alpha-2-macroglobulin KLK13 A2M Reconstituted Complex Kapadia C (2004) 14687906 Keratinocyte growth factor precursor Perlecan FGF7 HSPG2 Affinity Capture-Western Mongiat M (2000) 10702276 Keratinocyte growth factor precursor Perlecan FGF7 HSPG2 Reconstituted Complex Mongiat M (2000) 10702276 Keratinocyte growth factor precursor Perlecan FGF7 HSPG2 Two-hybrid Mongiat M (2000) 10702276 2 60/70kDa Amyloid KNS2 APP Reconstituted Complex Kamal A (2000) 11144355

Supplemental Table 6 91 Defining the elastic fiber interactome

emerin (Emery-Dreifuss muscular Lamin-A/C dystrophy) LMNA EMD Reconstituted Complex Clements L (2000) 10673356 Lamin-A/C HIV-1 Tat interactive protein LMNA HTATIP Two-hybrid Stelzl U (2005) 16169070 Lamin-A/C LMNA LMNB1 Two-hybrid Ostlund C (2001) 11792809 Lamin-A/C lamina associated polypeptide 1B LMNA LAP1B Reconstituted Complex Foisner R (1993) 8324822 Lamin-A/C NARF protein. LMNA NARF Two-hybrid Barton RM (1999) 10514485 Lamin-A/C Nesprin-1 LMNA SYNE1 Affinity Capture-Western Mislow JM (2002) 11801724 Lamin-A/C retinoblastoma 1 LMNA RB1 Reconstituted Complex Mancini MA (1994) 8278403 Lamin-A/C Retinoblastoma-associated protein LMNA RB1 Reconstituted Complex Mancini MA (1994) 8278403

Lamin-A/C Sterol regulatory element-binding protein 1 LMNA SREBF1 Affinity Capture-Western Lloyd DJ (2002) 11929849

Lamin-A/C Sterol regulatory element-binding protein 1 LMNA SREBF1 Reconstituted Complex Lloyd DJ (2002) 11929849

Lamin-A/C Sterol regulatory element-binding protein 1 LMNA SREBF1 Two-hybrid Lloyd DJ (2002) 11929849 Thymopoietin-related peptide isoforms Lamin-A/C beta/gamma LMNA TMPO Far Western Dechat T (2000) 10984438 Lamin-A/C Zinc finger protein LMNA ZNF239 Reconstituted Complex Dreuillet C (2002) 12409453 Lamin-A/C Zinc finger protein LMNA ZNF239 Two-hybrid Dreuillet C (2002) 12409453 Laminin subunit alpha-1 precursor Perlecan LAMA1 HSPG2 Reconstituted Complex Ettner N (1998) 9688542 Large proline-rich protein BAT2 Complement C1q BAT2 C1QBP Two-hybrid Lehner B (2004) 14667819 Leucine-rich repeat serine/threonine- protein kinase 1 [SP] Protein S100-A8 LRRK1 S100A8 Affinity Capture-MS Ewing RM (2007) 17353931 limb girdle muscular dystrophy 2G (autosomal reces Titin TCAP TTN Reconstituted Complex Mues A (1998) 9645487 limb girdle muscular dystrophy 2G (autosomal reces Titin TCAP TTN Two-hybrid Gregorio CC (1998) 9817758 lipoprotein-associated coagulation inhibitor |tiss Thrombospondin-1 TFPI THBS1 Reconstituted Complex Mast AE (2000) 10922378 Liprin-alpha-3 Alpha-2-macroglobulin LRP1 A2M Co-crystal Structure Dolmer K (2000) 10652313 Liprin-alpha-3 Amyloid LRP1 APBA2 Two-hybrid Gotthardt M (2000) 10827173

Liprin-alpha-3 Amyloid LRP1 APBB1 Two-hybrid Trommsdorff M (1998) 9837937 Liprin-alpha-3 Plasminogen activator inhibitor 1 LRP1 SERPINE1 Affinity Capture-Western Degryse B (2004) 15001579 Liprin-alpha-3 Thrombospondin-1 LRP1 THBS1 Reconstituted Complex Godyna S (1995) 7775583 Long-chain fatty acid transport protein 6 Vimentin SLC27A6 VIM Two-hybrid Stelzl U (2005) 16169070 Loricrin Vimentin LOR VIM Two-hybrid Stelzl U (2005) 16169070 low density lipoprotein-related protein 6 Dickkopf-related protein 1 LRP6 DKK1 Affinity Capture-Western Semenov MV (2001) 11448771 Low-density lipoprotein receptor-related protein 1 Alpha-2-macroglobulin LRP1 A2M Co-crystal Structure Dolmer K (2000) 10652313

Supplemental Table 6 92 Defining the elastic fiber interactome

Low-density lipoprotein receptor-related protein 1 Thrombospondin-1 LRP1 THBS1 Reconstituted Complex Godyna S (1995) 7775583 Low-density lipoprotein receptor-related protein 2 Serum albumin LRP2 ALB Reconstituted Complex Cui S (1996) 8898021 Low-density lipoprotein receptor-related protein 2 Thrombospondin-1 LRP2 THBS1 Reconstituted Complex Godyna S (1995) 7775583 LOXL Fibulin-5 LOXL1 FBLN5 Affinity Capture-Western Liu X (2004) 14745449 LOXL Fibulin-5 LOXL1 FBLN5 Reconstituted Complex Liu X (2004) 14745449 LOXL Fibulin-5 LOXL1 FBLN5 Two-hybrid Liu X (2004) 14745449 LTBP-2 Fibrillin-1 LTBP2 FBN1 Invitro Hirani R, et al. 17293099 Lysyl oxidase Elastin LOX ELN Invitro Choudhury R et al. 19570982 Lysyl oxidase Fibrillin-1 LOX FBN1 Invitro Choudhury R et al. 19570982 Lysyl oxidase Histone H1.5 LOX HIST1H1B Invitro Giampuzzi M et al. 12686141 Lysyl oxidase Histone H2B LOX HIST1H2BJ Invitro Giampuzzi M et al. 12686141 MAGP-1 Bone morphogenetic protein 7 MFAP2 BMP7 Invitro Weinbaum et al. 18625713 MAGP-1 Collagen alpha-3(IV) chain MFAP2 COL4A3 Reconstituted Complex Finnis ML (1997) 9278443 MAGP-1 dermatan sulphate proteoglycan I MFAP2 BGN Reconstituted Complex Reinboth B (2002) 11723132 MAGP-1 Fibrillin-2 MFAP2 FBN2 Invitro Hanssen et al. 15131124 MAGP-1 Protein jagged-1 MFAP2 JAG1 Reconstituted Complex Nehring LC et al. 15788413 MAGP-1 Protein jagged-2 MFAP2 JAG2 Reconstituted Complex Nehring LC et al. 15788413 MAGP-1 TGF beta-1 MFAP2 TGFB1 Invitro Weinbaum et al. 18625713 mannosidase, alpha type II-X Vimentin MAN2A2 VIM Two-hybrid Stelzl U (2005) 16169070 MAP kinase 2 Complement C1q MAPK1 C1QBP Biochemical Activity Majumdar M (2002) 11866440 MAP/ affinity-regulating kinase 4 Myosin-9 MARK4 MYH9 Affinity Capture-MS Trinczek B (2004) 14594945 Matrilin-2 MATN2 MATN3 Reconstituted Complex Frank S (2002) 11896063 Matrilin-2 Collagen alpha-1(I) chain MATN2 COL1A1 Reconstituted Complex Piecha D (2002) 12180907 Matrilin-2 Fibrillin-2 MATN2 FBN2 Reconstituted Complex Piecha D (2002) 12180907 Matrilin-2 Matrilin-2 MATN2 MATN2 Reconstituted Complex Piecha D (2002) 12180907 Matrilin-2 Matrilin-4 precursor. MATN2 MATN4 Reconstituted Complex Frank S (2002) 11896063 matrix metalloproteinase 9 (gelatinase B, 92kDa ge Thrombospondin-1 MMP9 THBS1 Affinity Capture-Western Bein K (2000) 10900205 meprin A, beta Fibronectin MEP1B FN1 Biochemical Activity Kaushal GP (1994) 8063866 metalloproteinase-like, disintegrin-like, cysteine-rich protein Stanniocalcin-2 ADAM11 STC2 Two-hybrid Stelzl U (2005) 16169070 mitochondrial ribosomal protein L44 Vimentin MRPL44 VIM Two-hybrid Stelzl U (2005) 16169070 Mitogen-activated protein kinase 3 Complement C1q MAPK3 C1QBP Biochemical Activity Majumdar M (2002) 11866440 MMP- X1 Complement C1q MMP14 C1QBP Protein-peptide Rozanov DV (2002) 12220632 Mob1 alpha [SP] Protein S100-A8 MOBK1B S100A8 Affinity Capture-MS Ewing RM (2007) 17353931 Moderately similar to PROTEIN

Supplemental Table 6 93 Defining the elastic fiber interactome

Moderately similar to PROTEIN PHOSPHATASE 2C ALPHA ISOFORM [H.sapiens] [GDB] Protein S100-A8 PPM1B S100A8 Affinity Capture-MS Ewing RM (2007) 17353931 Moesin-ezrin-radixin-like protein Spectrin beta chain brain 1 NF2 SPTBN1 Affinity Capture-Western Neill GW (2001) 11535133 Moesin-ezrin-radixin-like protein Spectrin beta chain brain 1 NF2 SPTBN1 Reconstituted Complex Neill GW (2001) 11535133 Moesin-ezrin-radixin-like protein Spectrin beta chain brain 1 NF2 SPTBN1 Two-hybrid Neill GW (2001) 11535133 Mono-ADP-ribosyltransferase sirtuin-6 Vimentin SIRT6 VIM Two-hybrid Stelzl U (2005) 16169070 Muscle-specific calcium-activated neutral protease Titin CAPN3 TTN Two-hybrid Ono Y (1998) 9642272 Muscle-specific calcium-activated neutral protease Titin CAPN3 TTN Two-hybrid Sorimachi H (1995) 8537379 Muscle-specific calcium-activated neutral protease 3 Titin CAPN3 TTN Two-hybrid Ono Y (1998) 9642272 Muscle-specific calcium-activated neutral protease 3 Titin CAPN3 TTN Two-hybrid Sorimachi H (1995) 8537379 myelin associated glycoprotein Collagen alpha-1(I) chain MAG COL1A1 Reconstituted Complex Fahrig T (1987) 2446864 myelin associated glycoprotein Collagen alpha-1(IV) chain MAG COL4A1 Reconstituted Complex Fahrig T (1987) 2446864 Myogenic repressor I-mf Dickkopf-related protein 1 MDFI DKK1 Two-hybrid Rual JF (2005) 16189514 myosin regulatory light chain 2, smooth Myosin-9 muscle isoform MYH9 MYL9 Two-hybrid Stelzl U (2005) 16169070 NARF protein. Lamin-A/C NARF LMNA Reconstituted Complex Barton RM (1999) 10514485 Nesprin-1 Lamin-A/C SYNE1 LMNA Reconstituted Complex Mislow JM (2002) 12163176

Neuroendocrine differentiation factor Insulin-like growth factor-binding protein 7 VPS24 IGFBP7 Affinity Capture-Western Wilson EM (2001) 11549700 Nidogen-1 precursor Fibulin-1 NID FBLN1 Reconstituted Complex Pan TC (1993) 8354280 Notch (Drosophila) homolog 1 (translocation-associ Protein NOV homolog NOTCH1 NOV Affinity Capture-Western Sakamoto K (2002) 12050162 Nuclear chloride ion channel 27 A-kinase anchor protein 9 CLIC1 AKAP9 Affinity Capture-Western Shanks RA (2002) 12163479 Nuclear factor NF- IL6 High mobility group protein CEBPB HMGA1 Affinity Capture-Western Foti D (2003) 12665574 nuclear factor of activated T-cells, preexisting component Vimentin NFATC2 VIM Two-hybrid Stelzl U (2005) 16169070

Nucleoside diphosphate kinase B Histone H2B NME2 HIST1H2BC Affinity Capture-MS Ewing RM (2007) 17353931 Nucleoside diphosphate kinase B Protein disulfide isomerase A6 NME2 PDIA6 Affinity Capture-MS Ewing RM (2007) 17353931 NY- REN-13 antigen Spectrin beta chain brain 1 CTNNA1 SPTBN1 Reconstituted Complex Pradhan D (2001) 11069925 Kontrogianni- Konstantopoulos A Obscurin ankyrin 1, erythrocytic OBSCN ANK1 Reconstituted Complex (2003) 12631729 overlapping activity with M-AAA protease Amyloid DAB1 APP Reconstituted Complex Homayouni R (1999) 10460257

Supplemental Table 6 94 Defining the elastic fiber interactome

PAI-1 mRNA binding protein Vimentin PAI-RBP1 VIM Two-hybrid Stelzl U (2005) 16169070 papillary serous carcinoma of the peritoneum Activated RNA polymerase II BRCA1 SUB1 Synthetic Rescue Bennett CB (2008) 18197258 Parotid salivary glycoprotein G1 Serum albumin PRB3 ALB Reconstituted Complex Hatton MN (1985) 4062880

Periplakin Vimentin PPL VIM Two-hybrid Kazerounian S (2002) 12366696 Paine-Saunders S et Perlecan Bone morphogenetic protein 2 HSPG2 BMP2 al. 11706034 Perlecan Collagen alpha-1(IV) chain HSPG2 COL4A1 Iozzo RV et al. 16064139 Perlecan Collagen alpha-1(XVIII) chain HSPG2 COL18A1 Invivo Miosge et al. 12588956 Perlecan Collagen alpha-2(IV) chain HSPG2 COL4A2 Iozzo RV et al. 16064139 Perlecan Connective tissue growth factor HSPG2 CTGF Invitro Nishida T 12811819 Perlecan FGF-1 HSPG2 FGF1 Invitro Knox S et al. 11747213 Perlecan FGF-18 HSPG2 FGF18 Invitro Melrose J et al. 16984910 Perlecan FGF-2 HSPG2 FGF2 Invitro Knox S et al. 11847221 Perlecan FGF-9 HSPG2 FGF9 Invitro Melrose J et al. 16984910 Perlecan Fibrillin-1 HSPG2 FBN1 Invitro Tiedemann K, et al. 15657057 Perlecan Fibrillin-1 HSPG2 FBN1 Invivo Tiedemann K, et al. 15657057 Perlecan Fibronectin HSPG2 FN1 Invitro Hopf M et al. 10092882 Perlecan Fibronectin HSPG2 FN1 Reconstituted Complex Hopf M (2001) 11493006 Perlecan fibulin 2 HSPG2 FBLN2 Reconstituted Complex Hopf M (2001) 11493006 Perlecan fibulin 2 HSPG2 FBLN2 Reconstituted Complex Sasaki T (1995) 7500359 Perlecan Granulins (Proepithelin) HSPG2 GRN Invitro Gonzalez EM et al. 12900424 Perlecan Integrin beta-1 HSPG2 ITGB1 Invitro Brown JC et al. 9431988 Perlecan leucine-rich repeat protein HSPG2 PRELP Invitro Bengtsson E et al. 11847210 Perlecan Nidogen-2 precursor HSPG2 NID2 Reconstituted Complex Hopf M (2001) 11493006 platelet-derived growth factor alpha Perlecan polypeptide HSPG2 PDGFA Reconstituted Complex Gohring W (1998) 9692901 Platelet-derived growth factor, beta Perlecan polypeptide ( HSPG2 PDGFB Reconstituted Complex Gohring W (1998) 9692901 transthyretin (prealbumin, amyloidosis type Perlecan I) HSPG2 TTR Reconstituted Complex Smeland S (1997) 9307034 Phosphoinositide phospholipase C Tyrosine-protein kinase BTK PLCG2 BTK Affinity Capture-Western Guo B (2000) 10981967 phospholipase A2 Vimentin YWHAZ VIM Affinity Capture-Western Tzivion G (2000) 10887173 1 Vimentin PKP1 VIM Reconstituted Complex Hofmann I (2000) 10852826 Plasminogen activator inhibitor 1 AGP 1 SERPINE1 ORM1 Reconstituted Complex Boncela J (2001) 11418606 Plasminogen activator inhibitor 1 AGP 1 SERPINE1 ORM1 Two-hybrid Boncela J (2001) 11418606 Plasminogen activator inhibitor 1 Insulin-like growth factor 5 SERPINE1 IGFBP5 Affinity Capture-Western Nam TJ (1997) 9202242

Plasminogen activator inhibitor 1 Insulin-like growth factor-binding protein 5 SERPINE1 IGFBP5 Affinity Capture-Western Nam TJ (1997) 9202242

Supplemental Table 6 95 Defining the elastic fiber interactome

Plasminogen activator inhibitor 1 plasminogen activator, tissue type SERPINE1 PLAT Reconstituted Complex Madison EL (1990) 2110366 Platelet-type lipoxygenase 12 Lamin-A/C ALOX12 LMNA Affinity Capture-Western Tang K (2000) 10727209 Platelet-type lipoxygenase 12 Lamin-A/C ALOX12 LMNA Two-hybrid Tang K (2000) 10727209 -1 Vimentin PLEC1 VIM Reconstituted Complex Herrmann H (1987) 3027087 polycystin 1 Collagen alpha-1(I) chain PKD1 COL1A1 Reconstituted Complex Malhas AN (2002) 11752017 polycystin 1 Fibronectin PKD1 FN1 Reconstituted Complex Malhas AN (2002) 11752017 polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa Stanniocalcin-2 POLR3F STC2 Two-hybrid Stelzl U (2005) 16169070 potassium voltage-gated channel, KQT- like subfamil A-kinase anchor protein 9 KCNQ1 AKAP9 Affinity Capture-Western Marx SO (2002) 11799244 potassium voltage-gated channel, KQT- like subfamil A-kinase anchor protein 9 KCNQ1 AKAP9 Reconstituted Complex Marx SO (2002) 11799244

POU domain, class 3, transcription factor 1 High mobility group protein POU3F1 HMGA1 Reconstituted Complex Leger H (1995) 7791781 PPH alpha Fibronectin MEP1A FN1 Biochemical Activity Kaushal GP (1994) 8063866 Pregnancy zone protein TGF beta-2 PZP TGFB2 Reconstituted Complex Philip A (1994) 7513640

Pregnancy-associated plasma protein-E1 Insulin-like growth factor-binding protein 3 PAPPA2 IGFBP3 Biochemical Activity Overgaard MT (2001) 11264294 Presenilin-binding protein Stanniocalcin-2 PBP STC2 Two-hybrid Stelzl U (2005) 16169070 Probable E3 ubiquitin-protein ligase TRIP12 Amyloid TRIP12 APPBP1 Affinity Capture-Western Park Y (2008) 18627766 Probable E3 ubiquitin-protein ligase TRIP12 Amyloid TRIP12 APPBP1 Biochemical Activity Park Y (2008) 18627766 programmed cell death 8 Stanniocalcin-2 PDCD8 STC2 Two-hybrid Stelzl U (2005) 16169070 Proliferating cell nuclear antigen Annexin A2 PCNA ANXA2 Affinity Capture-MS Ohta S (2002) 12171929 Proliferating cell nuclear antigen Annexin A2 PCNA ANXA2 Affinity Capture-Western Ohta S (2002) 12171929 proliferation-inducing protein 16 [EG] Fibulin-1 MPG FBLN1 Affinity Capture-MS Ewing RM (2007) 17353931 proliferation-inducing protein 42 Fibronectin REG3A FN1 Reconstituted Complex Christa L (1996) 8997243 proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) Vimentin PSME1 VIM Two-hybrid Stelzl U (2005) 16169070 proteasome nu chain Vimentin PSMA1 VIM Two-hybrid Rual JF (2005) 16189514 Protein 4.1 Myosin-9 EPB41 MYH9 Affinity Capture-MS Ewing RM (2007) 17353931 Protein inhibitor of neuronal nitric oxide synthas Vimentin DNCL1 Vim Affinity Capture-MS Navarro-Lerida I (2004) 14760703 protein kinase A, RII-alpha subunit A-kinase anchor protein 9 PRKAR2A AKAP9 Protein-peptide Alto NM (2003) 12672969 Protein kinase C alpha type Lamin-A/C PRKCA LMNA Protein-peptide Martelli AM (2002) 12112001 Protein kinase C epsilon type Myosin-9 PRKCE MYH9 Affinity Capture-MS England K (2002) 11897493 Protein kinase C epsilon type Myosin-9 PRKCE MYH9 Affinity Capture-Western England K (2002) 11897493 protein kinase C, mu Complement C1q PRKD1 C1QBP Affinity Capture-Western Storz P (2000) 10831594 protein kinase C, mu Complement C1q PRKD1 C1QBP Two-hybrid Storz P (2000) 10831594

Supplemental Table 6 96 Defining the elastic fiber interactome

Protein NOV homolog Fibronectin NOV FN1 Reconstituted Complex Ellis PD () 12902636 Protein NOV homolog Fibulin-1 NOV FBLN1 Two-hybrid Perbal B (1999) 9927660 Human RNA polymerase II subunit Protein NOV homolog (hsRPB10) mRNA, co NOV POLR2L Two-hybrid Perbal B (1999) 10474687 Protein NOV homolog Insulin precursor NOV INS Reconstituted Complex Burren CP (1999) 10084601 Protein NOV homolog Insulin-like growth factor IB precursor NOV IGF1 Reconstituted Complex Burren CP (1999) 10084601 Protein NOV homolog Insulin-like growth factor II NOV IGF2 Reconstituted Complex Burren CP (1999) 10084601 Protein NOV homolog integrin alpha-V NOV ITGAV Reconstituted Complex Ellis PD () 12902636 Notch (Drosophila) homolog 1 Protein NOV homolog (translocation-associ NOV NOTCH1 Affinity Capture-Western Sakamoto K (2002) 12050162 Notch (Drosophila) homolog 1 Protein NOV homolog (translocation-associ NOV NOTCH1 Reconstituted Complex Sakamoto K (2002) 12050162 regulatory inhibitor subunit 8 Stanniocalcin-2 PPP1R8 STC2 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A7 38 kDa Mov34 homolog S100A7 COPS5 Two-hybrid Emberley ED (2003) 12702588 -associated fatty acid-binding Protein S100-A7 protein ho S100A7 FABP5 Affinity Capture-Western Hagens G (1999) 10331666 Psoriasis-associated fatty acid-binding Protein S100-A7 protein ho S100A7 FABP5 Affinity Capture-Western Ruse M (2003) 12839573 Protein S100-A8 Alpha-tubulin 1 S100A8 Tuba1 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 Cellular tumor antigen p53 S100A8 TP53 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 14 open reading frame 1 S100A8 C14orf1 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 Growth/differentiation factor 9 precursor S100A8 GDF9 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 interferon receptor 1-bound protein 4 S100A8 HRMT1L2 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 mmunoglobulin superfamily member 21 S100A8 MGC15730 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 peptidylprolyl isomerase A S100A8 PPIA Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 Protein S100-A8 S100A8 S100A8 Co-crystal Structure Ishikawa K (2000) 10771424 Protein S100-A8 RRP5 protein homolog S100A8 PDCD11 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 Secretogranin I S100A8 CHGB Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 Telomere-associated protein RIF1 S100A8 RIF1 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 Tumor suppressor p53 S100A8 TP53 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A8 unc119 (C.elegans) homolog S100A8 UNC119 Two-hybrid Stelzl U (2005) 16169070 Protein S100-A9 Protein S100-A9 S100A9 S100A9 Co-crystal Structure Itou H (2002) 11851337 PROTHROMBIN PRECURSOR Plasminogen activator inhibitor 1 F2 SERPINE1 Reconstituted Complex Dekker RJ (2003) 12709053 Rab acceptor 1 Vimentin RABAC1 VIM Two-hybrid Stelzl U (2005) 16169070 RAB-8b protein Vimentin RAB8B VIM Affinity Capture-Western Lau AS (2003) 12639940 ran binding protein, centrosomal Protein S100-A7 RANBP9 S100A7 Reconstituted Complex Emberley ED (2002) 12421467 ran binding protein, centrosomal Protein S100-A7 RANBP9 S100A7 Two-hybrid Emberley ED (2002) 12421467 Ras-related C3 botulinum toxin substrate 1 precurs Dedicator of cytokinesis protein 2 RAC1 DOCK2 Reconstituted Complex Nishihara H (1999) 10559471

Supplemental Table 6 97 Defining the elastic fiber interactome

Retinoid X receptor alpha Insulin-like growth factor-binding protein 3 RXRA IGFBP3 Affinity Capture-Western Liu B (2000) 10874028

Retinoid X receptor alpha Insulin-like growth factor-binding protein 3 RXRA IGFBP3 Reconstituted Complex Liu B (2000) 10874028

Rho-type GTPase-activating protein 17 Tyrosine-protein kinase BTK ARHGAP17 BTK Reconstituted Complex Richnau N (2001) 11431473 Ro ribonucleoprotein-binding protein 1 [TR,EG] Titin SIAHBP1 TTN Affinity Capture-MS Ewing RM (2007) 17353931 Ro ribonucleoprotein-binding protein 1 [TR,EG] Vimentin SIAHBP1 VIM Two-hybrid Stelzl U (2005) 16169070 Ryanodine receptor 2 Ryanodine receptor 3 RYR2 RYR3 Affinity Capture-Western Xiao B (2002) 12213830 Ryanodine receptor 3 FK506-binding protein, T-cell, 12-kD RYR3 FKBP1A Reconstituted Complex Bultynck G (2001) 11598113 Ryanodine receptor 3 Ryanodine receptor 2 RYR3 RYR2 Affinity Capture-Western Xiao B (2002) 12213830 S. cerevisiaelike 2. Vimentin MGC42174 VIM Two-hybrid Stelzl U (2005) 16169070 salt tolerator A-kinase anchor protein 9 TRIP10 AKAP9 Reconstituted Complex Larocca MC (2004) 15047863 sarcomeric protein myopalladin, 145 kDa (MYOP) Titin MYPN TTN Two-hybrid Miller MK (2003) 14583192 serine/threonine protein kinase N A-kinase anchor protein 9 PKN1 AKAP9 Affinity Capture-Western Takahashi M (1999) 10358086 serine/threonine protein kinase N A-kinase anchor protein 9 PKN1 AKAP9 Two-hybrid Takahashi M (1999) 10358086 serine/threonine protein kinase N Vimentin PKN1 VIM Biochemical Activity Matsuzawa K (1997) 9175763 serine/threonine protein kinase N Vimentin PKN1 VIM Two-hybrid Matsuzawa K (1997) 9175763 Serine/threonine-protein kinase PAK 7 [SP] Protein S100-A7 PAK7 S100A7 Affinity Capture-MS Ewing RM (2007) 17353931 Serine/threonine-protein kinase PAK 7 [SP] Protein S100-A9 PAK7 S100A9 Affinity Capture-MS Ewing RM (2007) 17353931 Serine/threonine-protein kinase SMG1 delta helicase SMG1 RENT1 Biochemical Activity Yamashita A (2001) 11544179 Serine/threonine-protein kinase SMG1 delta helicase SMG1 RENT1 Protein-peptide Yamashita A (2001) 11544179 Serine/threonine-protein kinase SMG1 Serine/threonine-protein kinase SMG1 SMG1 SMG1 Biochemical Activity Yamashita A (2001) 11544179 Serologically defined breast cancer antigen NY-BR- Vimentin KIF15 VIM Two-hybrid Stelzl U (2005) 16169070 Serologically defined colon cancer antigen 3 Vimentin SDCCAG3 VIM Two-hybrid Stelzl U (2005) 16169070 Serotransferrin precursor Insulin-like growth factor II TF IGF2 Reconstituted Complex Storch S (2001) 11749962 Serum albumin LUC7-like 2 (S. cerevisiae) ALB LUC7L2 Two-hybrid Stelzl U (2005) 16169070 Serum albumin neonatal Fc-receptor ALB FCGRT Co-purification Chaudhury C (2003) 12566415 Serum albumin neonatal Fc-receptor ALB FCGRT Reconstituted Complex Chaudhury C (2003) 12566415 serum response factor (c-fos serum response elemen High mobility group protein SRF Hmga1 Phenotypic Enhancement Chin MT (1998) 9545312 serum response factor (c-fos serum

Supplemental Table 6 98 Defining the elastic fiber interactome

SH3-domain binding protein 5 (BTK- associated) Tyrosine-protein kinase BTK SH3BP5 BTK Affinity Capture-Western Yamadori T (1999) 10339589 SH3-domain binding protein 5 (BTK- associated) Tyrosine-protein kinase BTK SH3BP5 BTK Far Western Matsushita M (1998) 9571151 SH3-domain binding protein 5 (BTK- associated) Tyrosine-protein kinase BTK SH3BP5 BTK Reconstituted Complex Yamadori T (1999) 10339589 SH3YL1 protein. Vimentin SH3YL1 VIM Two-hybrid Stelzl U (2005) 16169070 SHC (Src homology 2 domain containing) transformin Amyloid SHC1 APP Reconstituted Complex Tarr PE (2002) 11877420 Single-stranded DNA-binding protein Single-stranded DNA-binding protein SSBP1 SSBP1 Co-crystal Structure Yang C (1997) 9033597 slit-like 2 (Drosophila) |slit-like 2 |SLITL2; ORF TGF beta-2 SLITL2 TGFB2 Reconstituted Complex Ikeda Y (2004) 15247411 Smooth muscle protein 22-alpha [SP] Protein S100-A9 TAGLN S100A9 Affinity Capture-MS Ewing RM (2007) 17353931 SMT3 homolog 1 Vimentin SUMO3 VIM Two-hybrid Stelzl U (2005) 16169070

SMT3 suppressor of mif two 3 homolog 2 Vimentin SUMO2 VIM Two-hybrid Stelzl U (2005) 16169070 Son of sevenless homolog 1 |son of sevenless homol Annexin A2 SOS1 ANXA2 Reconstituted Complex Buday L (1994) 7510700 Spectrin alpha chain brain Vimentin SPTAN1 VIM Affinity Capture-Western Brown MJ (2001) 11441066 Spectrin alpha chain, brain Vimentin SPTAN1 VIM Affinity Capture-Western Brown MJ (2001) 11441066 Spectrin beta chain brain 1 150 kDa dynein-associated polypeptide SPTBN1 DCTN1 Affinity Capture-Western Holleran EA (1996) 8991093 Spectrin beta chain brain 1 Band 4.1-like protein 3 SPTBN1 EPB41L3 Reconstituted Complex Robb VA (2004) 15116094 Spectrin beta chain brain 1 Glutamate receptor SPTBN1 GRIA2 Far Western Hirai H (1999) 10576550 Stanniocalcin-2 neurite growth-promoting factor 1 STC2 PTN Two-hybrid Stelzl U (2005) 16169070 sterile 20-like kinase 3 [EG] Apolipoprotein D STK24 APOD Affinity Capture-MS Ewing RM (2007) 17353931 syntaxin 1A (brain) |Neuron-specific antigen HPC-1 Vimentin STX1A VIM Two-hybrid Stelzl U (2005) 16169070 tal-1 product Single-stranded DNA-binding protein TAL1 SSBP1 Affinity Capture-MS Goardon N (2006) 16407974 HRIHFB212 tara-like protein Vimentin 2 VIM Two-hybrid Stelzl U (2005) 16169070 Tenascin TNC ITGA9 Affinity Capture-Western Young BA (2001) 11598204 Tenascin TNC ITGA9 Reconstituted Complex Young BA (2001) 11598204 Tenascin chondroitin sulfate proteoglycan 3 TNC CSPG3 Far Western Rauch U (1997) 9341124 TGase- H Fibronectin TGM2 FN1 Reconstituted Complex Heath DJ (2002) 11829448 TGF beta-2 TGFB2 DAXX Reconstituted Complex Perlman R (2001) 11483955 TGF beta-2 TGFB2 TGFBRAP1 Affinity Capture-Western Wurthner JU (2001) 11278302 TGF beta-2 dermatan sulphate proteoglycans II TGFB2 DCN Reconstituted Complex Schonherr E (1998) 9675033 TGF beta-2 transforming growth factor, beta 1 TGFB2 TGFB1 Two-hybrid Perlman R (2001) 11483955 Thioredoxin Collagen alpha-1(I) chain TXN COL1A1 Reconstituted Complex Matsumoto K (2002) 12099690 Thioredoxin Collagen alpha-1(I) chain TXN COL1A1 Two-hybrid Matsumoto K (2002) 12099690

Supplemental Table 6 99 Defining the elastic fiber interactome

Thrombospondin-1 Collagen alpha-1(I) chain THBS1 COL1A1 Reconstituted Complex Galvin NJ (1987) 3571333 Thrombospondin-1 Collagen alpha-1(II) chain THBS1 COL2A1 Reconstituted Complex Galvin NJ (1987) 3571333 Thrombospondin-1 Collagen alpha-1(III) chain THBS1 COL3A1 Reconstituted Complex Galvin NJ (1987) 3571333 Thrombospondin-1 Collagen alpha-1(IV) chain THBS1 COL4A1 Reconstituted Complex Galvin NJ (1987) 3571333 Thrombospondin-1 Collagen alpha-1(V) chain THBS1 COL5A1 Reconstituted Complex Galvin NJ (1987) 3571333 Thrombospondin-1 Collagen alpha-1(VII) chain THBS1 COL7A1 Two-hybrid Aho S (1998) 9840442 collagen, type III, alpha 1 (Ehlers-Danlos Thrombospondin-1 syndrom THBS1 COL3A1 Reconstituted Complex Galvin NJ (1987) 3571333

Thrombospondin-1 covering first half of fourth kringle THBS1 PLG Reconstituted Complex Silverstein RL (1984) 6438154 Thrombospondin-1 Fibrinogen alpha chain THBS1 FGA Reconstituted Complex Panetti TS (1999) 9867861 Thrombospondin-1 fibrinogen, alpha polypeptide THBS1 FGA Reconstituted Complex Panetti TS (1999) 9867861 fibroblast growth factor homologous factor Thrombospondin-1 2 THBS1 FGA Reconstituted Complex Panetti TS (1999) 9867861 Thrombospondin-1 Insulin-like growth factor 5 THBS1 IGFBP5 Reconstituted Complex Nam TJ (2000) 10698186

Thrombospondin-1 Insulin-like growth factor-binding protein 5 THBS1 IGFBP5 Reconstituted Complex Nam TJ (2000) 10698186 Thrombospondin-1 integrin beta chain, beta 3 THBS1 ITGB3 Reconstituted Complex Lawler J (1989) 2478219 Thrombospondin-1 leukocyte elastase THBS1 ELA2 Reconstituted Complex Hogg PJ (1993) 8463250 Thrombospondin-1 Liprin-alpha-3 THBS1 LRP1 Reconstituted Complex Mikhailenko I (1997) 9045712 Thrombospondin-1 Liprin-alpha-3 THBS1 LRP1 Reconstituted Complex Wang S (2004) 14991768 Low-density lipoprotein receptor-related Thrombospondin-1 protein 1 THBS1 LRP1 Reconstituted Complex Mikhailenko I (1997) 9045712 Low-density lipoprotein receptor-related Thrombospondin-1 protein 1 THBS1 LRP1 Reconstituted Complex Wang S (2004) 14991768 Thrombospondin-1 Perlecan THBS1 HSPG2 Invitro Feitsma K et al. 10734084 Platelet-derived growth factor, beta Thrombospondin-1 polypeptide ( THBS1 PDGFB Reconstituted Complex Hogg PJ (1997) 9334164 Thrombospondin-1 PROTHROMBIN PRECURSOR THBS1 F2 Reconstituted Complex Asch AS (1987) 2435757 Thrombospondin-1 TGF beta-1 THBS1 TGFB1 Invitro Ribeiro SM et al. 10224129 Thrombospondin-1 TGF beta-2 THBS1 TGFB2 Invitro Ribeiro SM et al. 10224129 Thymopoietin-related peptide isoforms beta/gamma Lamin-A/C TMPO LMNA Affinity Capture-Western Dechat T (2000) 10984438 Thymopoietin-related peptide isoforms beta/gamma Lamin-A/C TMPO LMNA Affinity Capture-Western Markiewicz E (2002) 12475961 Thymopoietin-related peptide isoforms beta/gamma Lamin-A/C TMPO LMNA Co-localization Dechat T (2000) 10984438 Thymopoietin-related peptide isoforms beta/gamma Lamin-A/C TMPO LMNA Far Western Dechat T (2000) 10984438

Supplemental Table 6 100 Defining the elastic fiber interactome

Titin ankyrin repeat domain 23 TTN ANKRD23 Reconstituted Complex Miller MK (2003) 14583192 Titin ankyrin repeat domain 23 TTN ANKRD23 Two-hybrid Miller MK (2003) 14583192

Titin Ankyrin repeat domain-containing protein 2 TTN ANKRD2 Reconstituted Complex Miller MK (2003) 14583192 Titin cardiomyopathy, hypertrophic 4 TTN MYBPC3 Far Western Freiburg A (1996) 8631348 limb girdle muscular dystrophy 2G Titin (autosomal reces TTN TCAP Biochemical Activity Mayans O (1998) 9804419 limb girdle muscular dystrophy 2G Titin (autosomal reces TTN TCAP Co-purification Zou P (2003) 12446666 limb girdle muscular dystrophy 2G Titin (autosomal reces TTN TCAP Two-hybrid Mues A (1998) 9645487 limb girdle muscular dystrophy 2G Titin (autosomal reces TTN TCAP Two-hybrid Zou P (2003) 12446666 Titin Obscurin TTN OBSCN Reconstituted Complex Young P (2001) 11448995 Titin Obscurin TTN OBSCN Two-hybrid Young P (2001) 11448995 Titin Protein MICAL-2 TTN MYBPC3 Far Western Freiburg A (1996) 8631348 Titin TRIM63 TTN TRIM63 Reconstituted Complex Centner T (2001) 11243782 Titin Ubiquitin ligase TRIM63 TTN TRIM63 Two-hybrid Centner T (2001) 11243782 Torsin-1A Lamin-A/C TOR1A LMNA Reconstituted Complex Foisner R (1993) 8324822 trabecular meshwork-induced glucocorticoid respons Fibrillin-1 MYOC FBN1 Reconstituted Complex Ueda J (2002) 11923248 trabecular meshwork-induced glucocorticoid respons Fibronectin MYOC FN1 Reconstituted Complex Filla MS (2002) 11773026 transcriptional intermediary factor 1-beta Vimentin TRIM28 VIM Two-hybrid Stelzl U (2005) 16169070 Transforming acidic coiled-coil-containing protein A-kinase anchor protein 9 TACC3 AKAP9 Two-hybrid Steadman BT (2002) 12015314 transforming growth factor beta receptor type II TGF beta-2 TGFBR2 TGFB2 Affinity Capture-Western Rotzer D (2001) 11157754 Murphy-Ullrich JE transforming growth factor, beta 1 Thrombospondin-1 TGFB1 THBS1 Co-purification (1992) 1550960 transthyretin (prealbumin, amyloidosis type I) Perlecan TTR HSPG2 Affinity Capture-Western Smeland S (1997) 9307034 transthyretin (prealbumin, amyloidosis type I) Vimentin TTR VIM Two-hybrid Stelzl U (2005) 16169070 Tribbles homolog 3 Fibronectin TRIB3 FN1 Affinity Capture-Western Zhou Y (2008) 18276110 Tribbles homolog 3 Fibronectin TRIB3 FN1 Two-hybrid Zhou Y (2008) 18276110

Supplemental Table 6 101 Defining the elastic fiber interactome

receptor superfamily, member 14 (herpes virus entry mediator) [GDB] Protein disulfide isomerase A6 TNFRSF14 PDIA6 Affinity Capture-MS Ewing RM (2007) 17353931 tumor necrosis factor receptor superfamily, member 14 (herpes virus entry mediator) [GDB] Vimentin TNFRSF14 VIM Two-hybrid Stelzl U (2005) 16169070 type 1 transmembrane glycoprotein interacting with Amyloid NCSTN APP Affinity Capture-Western Yu G (2000) 10993067 Tyrosine-protein kinase BTK ARID domain- containing protein 3A BTK ARID3A Affinity Capture-Western Nixon JC (2004) 15203319 Tyrosine-protein kinase BTK B-cell linker protein BTK BLNK Affinity Capture-MS Hashimoto S (1999) 10498607 Tyrosine-protein kinase BTK B-cell linker protein BTK BLNK Affinity Capture-Western Yasuda T (2002) 12093870 Bruton agammaglobulinemia tyrosine Tyrosine-protein kinase BTK kinase inhibitor BTK IBTK Reconstituted Complex Liu W (2001) 11577348 Cas-Br-M (murine) ecotropic retroviral Tyrosine-protein kinase BTK transforming sequence [GDB] BTK CBL Reconstituted Complex Cory GO (1995) 7629518 Tyrosine-protein kinase BTK Caveolin-1 BTK CAV1 Affinity Capture-Western Vargas L (2002) 11751885 dominant-negative kinase-deficient Tyrosine-protein kinase BTK Brutons tyrosin BTK BTK Co-crystal Structure Mao C (2001) 11527964 Tyrosine-protein kinase BTK E3 ubiquitin-protein ligase CBL-B BTK CBLB Affinity Capture-Western Yasuda T (2002) 12093870

Tyrosine-protein kinase BTK Ewing sarcoma breakpoint region 1 BTK EWSR1 Affinity Capture-Western Guinamard R (1997) 9201297

Tyrosine-protein kinase BTK EWS oncogene BTK EWSR1 Affinity Capture-Western Guinamard R (1997) 9201297 GAP- associated tyrosine phosphoprotein Tyrosine-protein kinase BTK p62 BTK KHDRBS1 Affinity Capture-Western Guinamard R (1997) 9201297 Tyrosine-protein kinase BTK General transcription factor II-I BTK GTF2I Affinity Capture-Western Sacristan C (2004) 14623887 Tyrosine-protein kinase BTK General transcription factor II-I BTK GTF2I Reconstituted Complex Sacristan C (2004) 14623887 Tyrosine-protein kinase BTK General transcription factor II-I BTK GTF2I Reconstituted Complex Yang W (1997) 9012831 Tyrosine-protein kinase BTK Guanine -binding protein G BTK GNAQ Affinity Capture-Western Ma YC (1998) 9770463 Tyrosine-protein kinase BTK Guanine nucleotide-binding protein G BTK GNAQ Reconstituted Complex Ma YC (1998) 9770463 Tyrosine-protein kinase BTK hemopoietic cell kinase BTK HCK Reconstituted Complex Cheng G (1994) 8058772 Tyrosine-protein kinase BTK p70 S6 kinase, alpha 2 BTK RPS6KB1 Biochemical Activity Rebholz H (2006) 16640565 Tyrosine-protein kinase BTK Phosphoinositide phospholipase C BTK PLCG2 Affinity Capture-Western Yasuda T (2002) 12093870 Tyrosine-protein kinase BTK Protein kinase C beta type BTK PRKCB1 Reconstituted Complex Yang XL (2003) 12679936 Tyrosine-protein kinase BTK protein kinase C, mu BTK PRKD1 Affinity Capture-Western Johannes FJ (1999) 10561498 Tyrosine-protein kinase BTK protein kinase C, mu BTK PRKD1 Reconstituted Complex Johannes FJ (1999) 10561498

Tyrosine-protein kinase BTK Proto-oncogene vav. BTK VAV1 Affinity Capture-Western Guinamard R (1997) 9201297 Tyrosine-protein kinase BTK Serine/threonine-protein kinase 14 beta BTK RPS6KB2 Biochemical Activity Rebholz H (2006) 16640565

Supplemental Table 6 102 Defining the elastic fiber interactome

Uncharacterized protein C7orf36. Vimentin C7orf36 VIM Two-hybrid Stelzl U (2005) 16169070 UPase 1 Vimentin UPP1 VIM Affinity Capture-MS Russell RL (2001) 11278417 UPase 1 Vimentin UPP1 VIM Co-purification Russell RL (2001) 11278417 Up-frameshift suppressor 2 homolog Serine/threonine-protein kinase SMG1 UPF2 SMG1 Affinity Capture-Western Yamashita A (2001) 11544179 Up-frameshift suppressor 3 homolog A Serine/threonine-protein kinase SMG1 UPF3A SMG1 Affinity Capture-Western Yamashita A (2001) 11544179 U-plasminogen activator Plasminogen activator inhibitor 1 PLAU SERPINE1 Reconstituted Complex Nykjaer A (1997) 9184208 upstream stimulatory factor 2 [EG] Protein S100-A8 USF2 S100A8 Affinity Capture-MS Ewing RM (2007) 17353931 upstream stimulatory factor 2 [EG] Protein S100-A9 USF2 S100A9 Affinity Capture-MS Ewing RM (2007) 17353931 uridine phosphorylase-2 Vimentin UPP2 VIM Affinity Capture-MS Russell RL (2001) 11278417 uridine phosphorylase-2 Vimentin UPP2 VIM Co-purification Russell RL (2001) 11278417 Vascular endothelial growth factor receptor 2 Annexin V KDR ANXA5 Reconstituted Complex Wen Y (1999) 10329451 Vascular endothelial growth factor receptor 2 Annexin V KDR ANXA5 Two-hybrid Wen Y (1999) 10329451 Vascular endothelial-cadherin Vimentin CDH5 VIM Affinity Capture-Western Shasby DM (2002) 12003790 v-crk sarcoma virus CT10 oncogene homolog (avian)- Dedicator of cytokinesis protein 2 CRKL DOCK2 Affinity Capture-Western Nishihara H (2002) 12393632 Versican core protein Fibrillin-1 CSPG2 FBN1 Reconstituted Complex Isogai Z (2002) 11726670 Vimentin Actin, alpha skeletal muscle VIM ACTA1 Affinity Capture-Western Brown MJ (2001) 11441066 Vimentin ALS2CR1; ORFNames=MDS015 VIM NIF3L1 Two-hybrid Rual JF (2005) 16189514 Amyotrophic lateral sclerosis 2 Vimentin chromosomal region VIM ALS2CR11 Two-hybrid Rual JF (2005) 16189514 Vimentin APAF1-interacting protein VIM MMRP19 Two-hybrid Rual JF (2005) 16189514 beaded filament structuralsprotein 1, Vimentin filensin VIM BFSP1 Reconstituted Complex Merdes A (1991) 1918147 Vimentin C- terminal LIM domain protein 1 VIM PDLIM1 Two-hybrid Rual JF (2005) 16189514 Vimentin CasL interacting molecule VIM MICAL1 Affinity Capture-Western Suzuki T (2002) 11827972 Vimentin downregulated in renal cell carcinoma VIM TU3A Two-hybrid Rual JF (2005) 16189514 Vimentin Fused in glioblastoma VIM GOPC Two-hybrid Rual JF (2005) 16189514 Vimentin Gamma-tubulin complex component 4 VIM 76P Two-hybrid Rual JF (2005) 16189514 Vimentin Keratin, type I cytoskeletal 20 VIM KRT20 Two-hybrid Rual JF (2005) 16189514 Vimentin KIAA0408 gene product VIM KIAA0408 Two-hybrid Rual JF (2005) 16189514 Vimentin lamin B1 VIM LMNB1 Affinity Capture-Western Brown MJ (2001) 11441066 Vimentin MYST protein 2 VIM MYST2 Two-hybrid Rual JF (2005) 16189514 Vimentin MYST protein 2 VIM MYST2 Two-hybrid Stelzl U (2005) 16169070 Vimentin NOC4-like protein VIM MGC3162 Two-hybrid Rual JF (2005) 16189514 Vimentin Nucleoside diphosphate kinase B VIM NME2 Affinity Capture-Western Roymans D (2000) 11082283 Vimentin oxysterol binding protein-like 1 VIM OSBP2 Co-fractionation Wang C (2002) 11802775 Vimentin phospholipase A2 VIM YWHAZ Affinity Capture-Western Tzivion G (2000) 10887173

Supplemental Table 6 103 Defining the elastic fiber interactome

Vimentin Spectrin alpha chain, brain VIM SPTAN1 Affinity Capture-Western Brown MJ (2001) 11441066 Vimentin Telomere-associated protein RIF1 VIM RIF1 Two-hybrid Stelzl U (2005) 16169070

Vimentin Trichoplein keratin filament-binding protein VIM MGC10854 Two-hybrid Rual JF (2005) 16189514 Vimentin Vimentin VIM VIM Co-crystal Structure Strelkov SV (2001) 11243787 Vimentin Vimentin VIM VIM Co-crystal Structure Strelkov SV (2002) 11889032 Vimentin Vimentin VIM VIM Two-hybrid Stelzl U (2005) 16169070 v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) Vimentin MAFG VIM Two-hybrid Stelzl U (2005) 16169070 von Willebrand factor Collagen alpha-1(I) chain VWF COL1A1 Co-fractionation Pareti FI (1986) 3490481 von Willebrand factor Collagen alpha-1(I) chain VWF COL1A1 Reconstituted Complex Pareti FI (1986) 3490481 Weakly similar to ANKYRIN, BRAIN VARIANT 2 [H.sapiens] [GDB] Protein S100-A8 ASB3 S100A8 Affinity Capture-MS Ewing RM (2007) 17353931 Weakly similar to ANKYRIN, BRAIN VARIANT 2 [H.sapiens] [GDB] Protein S100-A9 ASB3 S100A9 Affinity Capture-MS Ewing RM (2007) 17353931

Supplemental Table 6 104