www.impactjournals.com/oncotarget/ Oncotarget, Vol. 7, No. 32 Research Paper Induction of HOXA9 expression in three-dimensional organotypic culture of the Claudin-low breast cancer cells Miao Li1, Xi Li2, Yan Zhuang3, Yan Wang4, Matthew E. Burow3, Bridgette Collins- Burow3, Min Xue5, Chengjie Song5, Bin Shan6 1Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University, Shenyang, China 2Department of Sports Medicine and Joint Surgery, The People’s Hospital of Liaoning Province, Shenyang, China 3Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA 4Department of Biological Engineering, Zunyi Medical College Zhuhai Campus, Zhuhai, China 5Department of Physiology, Xuzhou Medical College, Xuzhou, China 6Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University Spokane, Spokane, WA, USA Correspondence to: Miao Li, email:
[email protected] Bin Shan, email:
[email protected] Keywords: breast cancer, extracellular matrix, three-dimensional organotypic culture, gene expression, homeobox gene Received: February 05, 2016 Accepted: June 29, 2016 Published: July 08, 2016 ABSTRACT The gene expression signatures of the molecular intrinsic subtypes of breast cancer are regulated by epigenetic mechanisms such as methylation of CpG islands in gene promoters. Epigenetic codes can be regulated by the tumor microenvironment. The Claudin-low subtype is associated with triple-negative invasive ductal carcinomas in patients. Herein we explored epigenetic regulation of gene expression in the Claudin-low breast cancer cells by extracellular matrix (ECM), a key component of the tumor microenvironment. We modeled attachment to ECM using laminin rich ECM three-dimensional organotypic culture (lrECM 3D). In 2D and lrECM 3D cultures we examined expression of the homeobox (HOX) genes that epigenetically regulated in development and cancer.