Evolution of mammalian Opn5 as a specialized UV-absorbing Title pigment by a single amino acid mutation. Yamashita, Takahiro; Ono, Katsuhiko; Ohuchi, Hideyo; Yumoto, Akane; Gotoh, Hitoshi; Tomonari, Sayuri; Sakai, Author(s) Kazumi; Fujita, Hirofumi; Imamoto, Yasushi; Noji, Sumihare; Nakamura, Katsuki; Shichida, Yoshinori Citation The Journal of biological chemistry (2014), 289(7): 3991-4000 Issue Date 2014-02-14 URL http://hdl.handle.net/2433/203075 This research was originally published in [ The Journal of Biological Chemistry, 289, 3991-4000. doi: Right 10.1074/jbc.M113.514075 © the American Society for Biochemistry and Molecular Biology Type Journal Article Textversion author Kyoto University Molecular property and expression pattern of mammalian Opn5 Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation* Takahiro Yamashita1,6, Katsuhiko Ono2,6, Hideyo Ohuchi3,6, Akane Yumoto1, Hitoshi Gotoh2, Sayuri Tomonari4, Kazumi Sakai1, Hirofumi Fujita3, Yasushi Imamoto1, Sumihare Noji4, Katsuki Nakamura5, and Yoshinori Shichida1 1Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, 2Department of Biology, Kyoto Prefectural University of Medicine, Kyoto 603-8334, Japan, 3Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan, 4Department of Life Systems, Institute of Technology and Science, University of Tokushima Graduate School, Tokushima 770-8506, Japan, 5Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan. *Running title: Molecular property and expression pattern of mammalian Opn5 Correspondence should be addressed to Y.S. (
[email protected]) 6These authors contributed equally to this work. Keywords: Rhodopsin; Photoreceptors; Signal transduction; G proteins; Molecular evolution; non- visual photoreception Background: Opn5 is considered to regulate non-visual photoreception in the retina and brain of animals.