HTRF Assays for Oncology and Inflammation

HTRF assays for Oncology and Inflammation

SIGNALING IN THE IMMUNE SYSTEM NK / NKT CELLS

Cytokines Markers GM-CSF (CSF2) IL10 Granzyme B IFNg TNFa Perforin-1

Pi3 Syk

ME SLP6 Iβ

ER C-Raf NFkB

Cisbio cell signaling assays available

IMMUNE CHECKPOINTS Biochemical assays to identify checkpoint blockers

TCR MHC II

CT C C

G C MC II

C SIα

enosine T CELL SIGNALING

TCR CD 4/8 CTLA4 PD1

CD3 CD28

CD40L AP0 SHP1

PLC SLP6 p38 c-Raf AT

IP1 ME GS3β NFAT Iβ

NFB

AP1

nhiitory INγ I I I I TNα

timulatory (Cisio cytokine assays availale) DNA Cisio cell signaling assays availale

pSLP6 Dexamethasone inhibitory eﬀect on CD3/CD28 stimulated PBMCs 8000 10000 ** hIFNγ L m

6000 /

g hIL2 p

s 5000 4000 e

n 2000 i k o t HTRF signal 2000 y C 1000 Neg

0 0 Basal 4 40 CD3/CD28 anti-CD3 Ab (g/mL) Deamethasone

Pathway monitoring in primary T cells upon CD3 stimulation Cytokine panel on PBMCs (300,000 cells per well, 5 min CD3 stimulation) B CELL SIGNALING

BCR

PD1 CD28 CTLA4 CD40L

SHP1 AT

PLC BLN Iβ GS3β c-Raf IP1

ME NFAT NFB

nhiitory AP1 INγ I I I I I TNα timulatory (Cisio cytokine assays availale) DNA Cisio cell signaling assays availale

Stimulation eﬀect of pervanadate on Raji cells Dexamethasone inhibitory eﬀect on LPS stimlated PBMCs B o H

T hIL6 L p R

s 3 m / F o

g hIL1β h R p P

t s o i i hTNFα o t e T a n i o R k

t o F a t y R l T C H 3 3 LPS Pervanadate (M) Deamethasone

Pervanadate effect on BTK accumulation (phospho & total) Cytokine panel on PBMCs INNATE IMMUNITY

Cytokines Inflammation markers IL1b CCL3 (MIP1a) Histamine IL6 CCL4 (MIP1b) PGE2 IL8 CCL5 (RANTES) LTC4 IFNb TNFa LTB4 CXCL10 (IP-10)

TLR5 TLR10 TLR2/TLR6 TLR1/TLR2 TLR4

dsDNA cGAS MYD88 ATP + GTP

2’-3’ cGAMP TBK1 IRAK3 IRAK1 IRAK2 STING IRAK4 IRF3 STING

p38 IKKβ TAK1

JNK

NFκB NFκB IRF3 IRF3 TNFα, IL6, IL1β Type-I-IFNs Cisio cell signaling assays availale

Toll-like receptor STING signaling pathway signaling pathway CELL-BASED ASSAYS CYTOKINE PHOSPHORYLATIONCELLULAR ASSAYS www.cisbio.com • • • • • • • • • • • Allow multiple cytokine measurements from 1sample from measurements cytokine multiple Allow plate low-volume 96-well Cisbio with Compatible Jurkat...) (THP1, lines cell and PBMCs on Validated time bench < 2hrs 16 volume μL Sample modulators allosteric and agonists, inhibitors, of pathway studies Pharmacological time < 1hrbench Blot Western than sensitive More 16 volume μL Sample models cell 3D cells, iPS extracts, tissue lysates, cell with Compatible assays Cell-based 3 yse timulate Plate Tdetectionantiodies +86 21 5018 9880 |[email protected] 21 5018 9880 +86 +33(0)466 796 705 |[email protected] 705 796 +33(0)466 1-888-963-4567 |[email protected] 1-888-963-4567 +81-(0)43-306-8712 | [email protected]+81-(0)43-306-8712 supernatant sample Transfer Europe and other countries U.S.A. and Canada Canada and U.S.A. Japan China T detection antiodies ncuate ncuate