Cytotoxic and Apoptogenic Effects of Strobilanthes Crispa Blume Extracts on Nasopharyngeal Cancer Cells
MOLECULAR MEDICINE REPORTS 12: 6293-6299, 2015 Cytotoxic and apoptogenic effects of Strobilanthes crispa Blume extracts on nasopharyngeal cancer cells RHUN YIAN KOH1, YI CHI SIM2, HWEE JIN TOH2, LIANG KUAN LIAM2, RACHAEL SZE LYNN ONG2, MEI YENG YEW3, YEE LIAN TIONG4, ANNA PICK KIONG LING1, SOI MOI CHYE1 and KHUEN YEN NG3 1Department of Human Biology, School of Medicine; 2School of Pharmacy and Health Sciences, International Medical University, Kuala Lumpur 57000; 3Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, Selangor 47500; 4School of Postgraduate Studies and Research, International Medical University, Kuala Lumpur 57000, Malaysia Received May 14, 2014; Accepted June 3, 2015 DOI: 10.3892/mmr.2015.4152 Abstract. The chemotherapeutic agents used to treat nasopha- classified into three subtypes: Squamous cell carcinoma, ryngeal cancer (NPC) exhibit low efficacy. Strobilanthes crispa non‑keratinizing carcinoma and undifferentiated carcinoma. Blume is widely used for its anticancer, diuretic and anti-diabetic The exact etiology of NPC remains to be elucidated, however, properties. The present study aimed to determine the cytotoxic it has been suggested that Epstein-Barr virus may be one of the and apoptogenic effects of S. crispa on CNE‑1 NPC cells. A causes of NPC, since it has been reported to be associated with 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide epithelial cell transformation into NPC type 2 and 3 (1,2). In assay was used to evaluate the cytotoxic effects of S. crispa addition, type 2 (non-keratinizing carcinoma) and 3 (undiffer- against CNE‑1 cells. The rate of apoptosis was determined entiated carcinoma) NPCs are associated with increased titers using propidium iodide staining and caspase assays.
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