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Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1986 Effect of Experimental Ostertagia Ostertagi Infection or Chronic Pentagastrin Treatment on Abomasal Pathophysiology and Morphology of Goats. Judit Eva Markovits Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Markovits, Judit Eva, "Effect of Experimental Ostertagia Ostertagi Infection or Chronic Pentagastrin Treatment on Abomasal Pathophysiology and Morphology of Goats." (1986). LSU Historical Dissertations and Theses. 4193. https://digitalcommons.lsu.edu/gradschool_disstheses/4193 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. 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Zeeb Road, Ann Arbor, Michigan 48106 8625344 Markovits, Judit Eva EFFECT OF EXPERIMENTAL OSTERTAGIA OSTERTAGI INFECTION OR CHRONIC PENTAGASTRIN TREATMENT ON ABOMASAL PATHOPHYSIOLOGY AND MORPHOLOGY OF GOATS The Louisiana State University and Agricultural and Mechanical Ph.D. Col. 1986 University Microfilms International300 N. Zeeb Road, Ann Arbor, Ml 48106 PLEASE NOTE: In all cases this material has been filmed in the best possible way from the available copy. Problems encountered with this document have been identified here with a check mark V 1. Glossy photographs or pagesJ 2. Colored illustrations, paper or______ print 3. Photographs with dark backgroundJ 4. Illustrations are poor copy______ 5. Pages with black marks, not original______ copy 6. Print shows through as there is text on both sides______ of page 7. Indistinct, broken or small print on several pages 8. Print exceeds margin requirements_____ 9. Tightly bound copy with print lost_____ In spine 10. Computer printout pages with indistinct___ print 11. Page(s) lacking when material received, and not available from school or author. 12. Page(s)___________ seem to be missing in numbering only as text follows. 13. Two pages numbered . Text follows. 14. Curling and wrinkled pages______ 15. Dissertation contains pages with print at a slant, filmed as received 16. Other___________________________________________________ University Microfilms International EFFECT OF EXPERIMENTAL OSTERTAGIA OSTERTAGI INFECTION OR CHRONIC PENTAGASTRIN TREATMENT ON ABOMASAL PATHOPHYSIOLOGY AND MORPHOLOGY OF GOATS A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Interdepartmental Program in Veterinary Medical Sciences Veterinary Pathology by Judit E. Markovits D.V.M., University of Veterinary Science, Hungary, 1978 August 1986 ACKNOWLEDGEMENTS I wish to express my gratitude to Dr. T.G. Snider who awakened my interest in gastroenterology and was also instrumental to gain the support of Ayerst Laboratories. Dr. Snider also gave guidance in the preparation of this dissertation. I am indebted to Dr. W.G. Henk for his suggestions and help in morphometric and electron microscopic techniques. I would also like to thank the other members of my graduate committee, Drs. H.W. Taylor, J.C. Williams and K.A. Gossett for their advice and editorial comments. A special thanks is due to Dr. H.W. Casey whose advice was invaluable throughout this study. I would like to thank' Dr. K.J. Wolfsheimer who gave excellent advice on the RIA technique. I also thank the support of Drs. D.C. Baker and S.D. Gaunt throughout this experiment. Dr. A.M. Saxton was helpful in the statistical evaluation of morphometry. Terry Romaire shared with me the responsibilities of taking care of the animals. I am also very grateful for Terry's editorial comments. All my light microscopic tissues were processed by Cheryl Crowder, whom I have to thank for her patience and talent during the frustrating months of modification of special stains. Without the efforts of Dr. V.J. Banks and Pam Ellis the morphometry would have been impossible to complete. Gary Bates. Rick Knight, Kenny Marbury, Rick Ramsey and Ted Vance helped on the farm. Serum chemistry and hematology was performed by Maureen Stockwell, Carol Turnwald and Linda West. Sherry Gibson and Laura Tounger had some excellent practical suggestion on EM techniques. I wish to thank Jason Osburn for his help with parasite recovery. Andy Smith made contact prints and developed film for me. Michael Broussard and Harry Cowgill were helpful with illustrations for the dissertation and seminars. This work was supported by a grant from the School of Veterinary Medicine Organized Research Fund, Louisiana State University. Additional support was provided by Ayerst Laboratories, New York, NY. iii Table of Contents Pa.fi e Acknowledgements .. ........................... ....11 Table of Contents. ........... .............. iv List of Figures...............................................v List of Tables .................... ix Abstract.................. xi Chapter I - Literature Review and Objectives ....... 1 Chapter II - Experimental Ostertagia ostertagi Infection or Chronic Pentagastrin Treatment of Goat Kids: Parasitological, Clinicopathologic and Macroscopic Observations Introduction. ............... 29 Materials and Methods........... 31 Results ................................................... 39 Discussion.................... 43 Chapter III - Qualitative and Quantitaive Light Microscopic Studies Following Ostertagia ostertagi Infection or Chronic Pentagastrin Treatment of Goat Kids Introduction................................................. 66 Materials and Methods................................ .. 67 Results................................ 73 Discussion.............................................. • 80 Chapter IV - U1trastructural Abomasal Changes Following Ostertagia ostertagi Infection or Chronic Pentagastrin Treatment of Goat Kids Introduction.................. 107 Materials and Methods ................................... 108 Results.....................................................113 Discussion....................... 119 Chapter V - Summary and Conclusions.................... 147 Bibliography................................................ 152 Vita........................... 172 Approval Sheets iv LIST OF FIGURES Page CHAPTER II Fig. 1 Gastrin RIA kit validation, specificity . 56 Fig. 2 Serum pepsinogen levels of Group I control goat kids .................... 57 Fig. 3 Serum pepsinogen levels of Group III goat k i d s ................................ 58 Fig. A Serum pepsinogen levels of Group II goat k i d s ...................................59 Fig. 5 Gastrin immunoreactivity of Group I control goat k i d s .......................... 60 Fig. 6 Gastrin immunoreactivity of Group III goat kids ........................... .61 Fig. 7 Gastrin immunoreactivity of Group II goat k i d s ....................................62 Fig. 8 Abomasa of control and Ostertagia ostertagi-infected goat kids opened along the greater curvature............... 63 Fig. 9 Abomasum of an Ostertagia ostertagi- infected goat k i d .......................... 64 Fig. 10 Abomasum of a pentagastrin-treated goat kid. ................................65 CHAPTER III Fig. 1 Schematic drawing of a caprine abomasum opened along the greater curvature........ 90 Fig. 2 Non-folded fundus of a control animal .. 91 Fig. 3 Fundic fold of a control a n i m a l ...........92 Fig. A Non-folded fundus of a pentagastrin- treated animal............................... 93 Fig. 5 Fundic fold of a pentagastrin- treated animal............. .........94 v CHAPTER III Con't Fig. 6 Non-folded fundus of an 0 ostertaRi- infected animal ......................... Fig.

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