Reconstructing the Evolution of Metabolism in Budding Yeasts
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Fungal Evolutionary Genomics Provides Insight Into The
Fungal evolutionary genomics provides insight into the mechanisms of adaptive divergence in eukaryotes Pierre Gladieux, Jeanne Ropars, Helene Badouin, Antoine Branca, Gabriela Aguileta, Damien de Vienne, Ricardo C. Rodriguez de la Vega, Sara Branco, Tatiana Giraud To cite this version: Pierre Gladieux, Jeanne Ropars, Helene Badouin, Antoine Branca, Gabriela Aguileta, et al.. Fungal evolutionary genomics provides insight into the mechanisms of adaptive divergence in eukaryotes. Molecular Ecology, Wiley, 2014, 23 (4), pp.753-773. 10.1111/mec.12631. hal-01302695 HAL Id: hal-01302695 https://hal.archives-ouvertes.fr/hal-01302695 Submitted on 14 Apr 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Molecular Ecology (2014) 23, 753–773 doi: 10.1111/mec.12631 INVITED REVIEWS AND SYNTHESES Fungal evolutionary genomics provides insight into the mechanisms of adaptive divergence in eukaryotes PIERRE GLADIEUX,*†‡ JEANNE ROPARS,*† HELENE BADOUIN,*† ANTOINE BRANCA,*† GABRIELA AGUILETA,§¶ DAMIEN M. DE VIENNE,§¶** RICARDO C. RODRIGUEZ DE LA VEGA,*† SARA BRANCO‡ -
A Single Gal4-Like Transcription Factor Activates the Crabtree Effect in Komagataella Phaffii
ARTICLE DOI: 10.1038/s41467-018-07430-4 OPEN A single Gal4-like transcription factor activates the Crabtree effect in Komagataella phaffii Özge Ata1,2, Corinna Rebnegger1,3, Nadine E. Tatto1,4,5, Minoska Valli1,4, Teresa Mairinger4,6,8, Stephan Hann4,6, Matthias G. Steiger 1,4,Pınar Çalık 2,7 & Diethard Mattanovich 1,4 The Crabtree phenotype defines whether a yeast can perform simultaneous respiration and fermentation under aerobic conditions at high growth rates. It provides Crabtree positive 1234567890():,; yeasts an evolutionary advantage of consuming glucose faster and producing ethanol to outcompete other microorganisms in sugar rich environments. While a number of genetic events are associated with the emergence of the Crabtree effect, its evolution remains unresolved. Here we show that overexpression of a single Gal4-like transcription factor is sufficient to convert Crabtree-negative Komagataella phaffii (Pichia pastoris) into a Crabtree positive yeast. Upregulation of the glycolytic genes and a significant increase in glucose uptake rate due to the overexpression of the Gal4-like transcription factor leads to an overflow metabolism, triggering both short-term and long-term Crabtree phenotypes. This indicates that a single genetic perturbation leading to overexpression of one gene may have been sufficient as the first molecular event towards respiro-fermentative metabolism in the course of yeast evolution. 1 Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria. 2 Department of Biotechnology, Graduate School of Natural and Applied Sciences, Middle East Technical University, 06800 Ankara, Turkey. 3 CD-Laboratory for Growth-Decoupled Protein Production in Yeast, Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria. -
Phylogenetic Circumscription of Saccharomyces, Kluyveromyces
FEMS Yeast Research 4 (2003) 233^245 www.fems-microbiology.org Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora Cletus P. Kurtzman à Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, Peoria, IL 61604, USA Received 22 April 2003; received in revised form 23 June 2003; accepted 25 June 2003 First published online Abstract Genera currently assigned to the Saccharomycetaceae have been defined from phenotype, but this classification does not fully correspond with species groupings determined from phylogenetic analysis of gene sequences. The multigene sequence analysis of Kurtzman and Robnett [FEMS Yeast Res. 3 (2003) 417^432] resolved the family Saccharomycetaceae into 11 well-supported clades. In the present study, the taxonomy of the Saccharomyctaceae is evaluated from the perspective of the multigene sequence analysis, which has resulted in reassignment of some species among currently accepted genera, and the proposal of the following five new genera: Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. ß 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Keywords: Saccharomyces; Kluyveromyces; New ascosporic yeast genera; Molecular systematics; Multigene phylogeny 1. Introduction support the maintenance of three distinct genera. Yarrow [8^10] revived the concept of three genera and separated The name Saccharomyces was proposed for bread and Torulaspora and Zygosaccharomyces from Saccharomyces, beer yeasts by Meyen in 1838 [1], but it was Reess in 1870 although species assignments were often di⁄cult. -
Modeling of Overflow Metabolism in Batch and Fed-Batch Cultures of Escherichia Coli
Biotechnol. Prog. 1999, 15, 81−90 81 Modeling of Overflow Metabolism in Batch and Fed-Batch Cultures of Escherichia coli Bo Xu, Mehmedalija Jahic, and Sven-Olof Enfors* Department of Biotechnology, Royal Institute of Technology, S-100 44 Stockholm, Sweden A dynamic model of glucose overflow metabolism in batch and fed-batch cultivations of Escherichia coli W3110 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and acetate formation as well as acetate reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. E. coli excreted acetate only when specific glucose uptake exceeded a critical rate corresponding to a maximum respiration rate. In batch cultures where the glucose uptake was unlimited, the overflow acetate made up to 9.0 ( 1.0% carbon/ carbon of the glucose consumed. The applicability of the model to dynamic situations was tested by challenging the model with glucose and acetate pulses added during the fed-batch part of the cultures. In the presence of a glucose feed, E. coli utilized acetate 3 times faster than in the absence of glucose. The cells showed no significant difference in maximum specific uptake rate of endogenous acetate produced by glucose overflow and exogenous acetate added to the culture, the value being 0.12-0.18 g g-1 h-1 during the entire fed-batch culture period. Acetate inhibited the specific growth rate according to a noncompetitive model, with the inhibition constant (ki) being 9 g of acetate/L. This was due to the reduced rate of glucose uptake rather than the reduced yield of biomass. -
Candida Albicans from Wikipedia, the Free Encyclopedia
Candida albicans From Wikipedia, the free encyclopedia Candida albicans is a type of yeast that is a common member of the human gut flora. It does not proliferate outside the human body.[4] It is Candida albicans detected in the gastrointestinal tract and mouth in 40-60% of healthy adults.[5][6] It is usually a commensal organism, but can become pathogenic in immunocompromised individuals under a variety of conditions.[6][7] It is one of the few species of the Candida genus that causes the human infection candidiasis, which results from an overgrowth of the fungus.[6][7] Candidiasis is for example often observed in HIV-infected patients.[8] C. albicans is the most common fungal species isolated from biofilms either formed on (permanent) implanted medical devices or on human Candida albicans visualised using [9][10] tissue. C. albicans, together with C. tropicalis, C. parapsilosis scanning electron microscopy. Note the and C. glabrata, is responsible for 50–90% of all cases of candidiasis in abundant hypal mass. humans.[7][11][12] A mortality rate of 40% has been reported for patients with systemic candidiasis due to C. albicans.[13] Estimates Scientific classification range from 2800 to 11200 deaths caused annually in the USA due to C. Kingdom: Fungi albicans causes candidiasis.[14] Division: Ascomycota C. albicans is commonly used as a model organism for biology. It is generally referred to as a dimorphic fungus since it grows both as yeast Class: Saccharomycetes and filamentous cells. However it has several different morphological Order: Saccharomycetales phenotypes. C. albicans was for a long time considered an obligate diploid organism without a haploid stage. -
Regulation of Amino-Acid Metabolism Controls Flux to Lipid Accumulation in Yarrowia Lipolytica
View metadata,Downloaded citation and from similar orbit.dtu.dk papers on:at core.ac.uk Nov 09, 2017 brought to you by CORE provided by Online Research Database In Technology Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica Kerkhoven, Eduard J.; Pomraning, Kyle R.; Baker, Scott E.; Nielsen, Jens Published in: n p j Systems Biology and Applications Link to article, DOI: 10.1038/npjsba.2016.5 Publication date: 2016 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Kerkhoven, E. J., Pomraning, K. R., Baker, S. E., & Nielsen, J. (2016). Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica. n p j Systems Biology and Applications, 2, [16005]. DOI: 10.1038/npjsba.2016.5 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. www.nature.com/npjsba All rights reserved 2056-7189/16 ARTICLE OPEN Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica Eduard J Kerkhoven1, Kyle R Pomraning2, Scott E Baker2 and Jens Nielsen1,3 Yarrowia lipolytica is a promising microbial cell factory for the production of lipids to be used as fuels and chemicals, but there are few studies on regulation of its metabolism. -
Citric Acid and Itaconic Acid Accumulation: Variations of the Same Story?
Applied Microbiology and Biotechnology https://doi.org/10.1007/s00253-018-09607-9 MINI-REVIEW Citric acid and itaconic acid accumulation: variations of the same story? Levente Karaffa 1 & Christian P. Kubicek2,3 Received: 5 December 2018 /Revised: 28 December 2018 /Accepted: 28 December 2018 # The Author(s) 2019 Abstract Citric acid production by Aspergillus niger and itaconic acid production by Aspergillus terreus are two major examples of technical scale fungal fermentations based on metabolic overflow of primary metabolism. Both organic acids are formed by the same metabolic pathway, but whereas citric acid is the end product in A. niger, A. terreus performs two additional enzymatic steps leading to itaconic acid. Despite of this high similarity, the optimization of the production process and the mechanism and regulation of overflow of these two acids has mostly been investigated independently, thereby ignoring respective knowledge from the other. In this review, we will highlight where the similarities and the real differences of these two processes occur, which involves various aspects of medium composition, metabolic regulation and compartmentation, transcriptional regulation, and gene evolution. These comparative data may facilitate further investigations of citric acid and itaconic acid accumulation and may contribute to improvements in their industrial production. Keywords Aspergillus niger . Aspergillus terreus . Citric acid . Itaconic acid . Submerged fermentation . Overflow metabolism Introduction terreus—was patented in the next decade (Kane et al. 1945). Before World War II, organic acid manufacturing was exclu- Citric acid (2-hydroxy-propane-1,2,3-tricarboxylic acid) sively performed by the labor-intensive and relatively low- and itaconic acid (2-methylene-succinic acid or 2- yield surface method (Doelger and Prescott 1934;Calam methylidenebutanedioic acid) are the most well-known exam- et al. -
Complete Genome Sequence and the Expression Pattern of Plasmids Of
Yang et al. Biotechnol Biofuels (2018) 11:125 https://doi.org/10.1186/s13068-018-1116-x Biotechnology for Biofuels RESEARCH Open Access Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose‑utilizing derivatives 8b and 2032 Shihui Yang1,2*† , Jessica M. Vera3†, Jef Grass3, Giannis Savvakis4, Oleg V. Moskvin3, Yongfu Yang1, Sean J. McIlwain3, Yucai Lyu3,8, Irene Zinonos4, Alexander S. Hebert3, Joshua J. Coon3, Donna M. Bates3, Trey K. Sato3, Steven D. Brown5,6,9, Michael E. Himmel7, Min Zhang2, Robert Landick3, Katherine M. Pappas4* and Yaoping Zhang3*† Abstract Background: Zymomonas mobilis is a natural ethanologen being developed and deployed as an industrial biofuel producer. To date, eight Z. mobilis strains have been completely sequenced and found to contain 2–8 native plasmids. However, systematic verifcation of predicted Z. mobilis plasmid genes and their contribution to cell ftness has not been hitherto addressed. Moreover, the precise number and identities of plasmids in Z. mobilis model strain ZM4 have been unclear. The lack of functional information about plasmid genes in ZM4 impedes ongoing studies for this model biofuel-producing strain. Results: In this study, we determined the complete chromosome and plasmid sequences of ZM4 and its engineered xylose-utilizing derivatives 2032 and 8b. Compared to previously published and revised ZM4 chromosome sequences, the ZM4 chromosome sequence reported here contains 65 nucleotide sequence variations as well as a 2400-bp insertion. Four plasmids were identifed in all three strains, with 150 plasmid genes predicted in strain ZM4 and 2032, and 153 plasmid genes predicted in strain 8b due to the insertion of heterologous DNA for expanded substrate utilization. -
Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella Bryantii B14
proteomes Article Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella bryantii B14 Andrej Trautmann 1, Lena Schleicher 2, Simon Deusch 1 , Jochem Gätgens 3 , Julia Steuber 2 and Jana Seifert 1,* 1 Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany; [email protected] (A.T.); [email protected] (S.D.) 2 Institute of Biology, University of Hohenheim, 70599 Stuttgart, Germany; [email protected] (L.S.); [email protected] (J.S.) 3 Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425 Jülich, Germany; [email protected] * Correspondence: [email protected] Received: 22 September 2020; Accepted: 13 October 2020; Published: 16 October 2020 Abstract: Short-chain fatty acids (SCFAs) are bacterial products that are known to be used as energy sources in eukaryotic hosts, whereas their role in the metabolism of intestinal microbes is rarely explored. In the present study, acetic, propionic, butyric, isobutyric, valeric, and isovaleric acid, respectively, were added to a newly defined medium containing Prevotella bryantii B14 cells. After 8 h and 24 h, optical density, pH and SCFA concentrations were measured. Long-chain fatty acid (LCFA) profiles of the bacterial cells were analyzed via gas chromatography-time of flight-mass spectrometry (GC-ToF MS) and proteins were quantified using a mass spectrometry-based, label-free approach. Cultures supplemented with single SCFAs revealed different growth behavior. Structural features of the respective SCFAs were identified in the LCFA profiles, which suggests incorporation into the bacterial membranes. The proteomes of cultures supplemented with acetic and valeric acid differed by an increased abundance of outer membrane proteins. -
Downloaded from NCBI Genbank Or Sequence
Lind and Pollard Microbiome (2021) 9:58 https://doi.org/10.1186/s40168-021-01015-y METHODOLOGY Open Access Accurate and sensitive detection of microbial eukaryotes from whole metagenome shotgun sequencing Abigail L. Lind1 and Katherine S. Pollard1,2,3,4,5* Abstract Background: Microbial eukaryotes are found alongside bacteria and archaea in natural microbial systems, including host-associated microbiomes. While microbial eukaryotes are critical to these communities, they are challenging to study with shotgun sequencing techniques and are therefore often excluded. Results: Here, we present EukDetect, a bioinformatics method to identify eukaryotes in shotgun metagenomic sequencing data. Our approach uses a database of 521,824 universal marker genes from 241 conserved gene families, which we curated from 3713 fungal, protist, non-vertebrate metazoan, and non-streptophyte archaeplastida genomes and transcriptomes. EukDetect has a broad taxonomic coverage of microbial eukaryotes, performs well on low-abundance and closely related species, and is resilient against bacterial contamination in eukaryotic genomes. Using EukDetect, we describe the spatial distribution of eukaryotes along the human gastrointestinal tract, showing that fungi and protists are present in the lumen and mucosa throughout the large intestine. We discover that there is a succession of eukaryotes that colonize the human gut during the first years of life, mirroring patterns of developmental succession observed in gut bacteria. By comparing DNA and RNA sequencing of paired samples from human stool, we find that many eukaryotes continue active transcription after passage through the gut, though some do not, suggesting they are dormant or nonviable. We analyze metagenomic data from the Baltic Sea and find that eukaryotes differ across locations and salinity gradients. -
Recent Progress in the Microbial Production of Pyruvic Acid
fermentation Review Recent Progress in the Microbial Production of Pyruvic Acid Neda Maleki 1 and Mark A. Eiteman 2,* 1 Department of Food Science, Engineering and Technology, University of Tehran, Karaj 31587-77871, Iran; [email protected] 2 School of Chemical, Materials and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA * Correspondence: [email protected]; Tel.: +1-706-542-0833 Academic Editor: Gunnar Lidén Received: 10 January 2017; Accepted: 6 February 2017; Published: 13 February 2017 Abstract: Pyruvic acid (pyruvate) is a cellular metabolite found at the biochemical junction of glycolysis and the tricarboxylic acid cycle. Pyruvate is used in food, cosmetics, pharmaceutical and agricultural applications. Microbial production of pyruvate from either yeast or bacteria relies on restricting the natural catabolism of pyruvate, while also limiting the accumulation of the numerous potential by-products. In this review we describe research to improve pyruvate formation which has targeted both strain development and process development. Strain development requires an understanding of carbohydrate metabolism and the many competing enzymes which use pyruvate as a substrate, and it often combines classical mutation/isolation approaches with modern metabolic engineering strategies. Process development requires an understanding of operational modes and their differing effects on microbial growth and product formation. Keywords: auxotrophy; Candida glabrata; Escherichia coli; fed-batch; metabolic engineering; pyruvate; pyruvate dehydrogenase 1. Introduction Pyruvic acid (pyruvate at neutral pH) is a three carbon oxo-monocarboxylic acid, also known as 2-oxopropanoic acid, 2-ketopropionic acid or acetylformic acid. Pyruvate is biochemically located at the end of glycolysis and entry into the tricarboxylic acid (TCA) cycle (Figure1). -
Modelling Overflow Metabolism in Escherichia Coli with Flux Balance
Zeng and Yang BMC Systems Biology (2019)13:3 https://doi.org/10.1186/s12918-018-0677-4 RESEARCH ARTICLE Open Access Modelling overflow metabolism in Escherichia coli with flux balance analysis incorporating differential proteomic efficiencies of energy pathways Hong Zeng and Aidong Yang* Abstract Background: The formation of acetate by fast-growing Escherichia coli (E. coli) is a commonly observed phenomenon, often referred to as overflow metabolism. Among various studies that have been carried over decades, a recent work (Basan, M. et al. Nature 528, 99–104, 2015) suggested and validated that it is the differential proteomic efficiencies in energy biogenesis between fermentation and respiration that lead to the production of acetate at rapid growth conditions, as the consequence of optimally allocating the limited proteomic resource. In the current work, we attempt to incorporate this newly developed proteome allocation theory into flux balance analysis (FBA) to capture quantitatively the extent of overflow metabolism in different E. coli strains. Results: A concise constraint was introduced into a FBA-based model with three proteomic cost parameters to represent constrained allocation of proteome over two energy (respiration and fermentation) pathways and biomass synthesis. Linear relationships were shown to exist between the three proteomic cost parameters. Tests with three different strains revealed that the proteomic cost of fermentation was consistently lower than that of respiration. A slow-growing strain appeared to have a higher proteomic cost for biomass synthesis than fast-growing strains. Different assumed levels of carbon flowing into pentose phosphate pathway affected the absolute value of model parameters, but had no qualitative impact on the comparative proteomic costs.