Detection and Quantification of Phytophthora Species Which Are
Eur. J. For. Path. 29 (1999) 169–188 © 1999 Blackwell Wissenschafts-Verlag, Berlin ISSN 0300–1237 Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction 1 2 3 3 4 By R. SCHUBERT *, G. BAHNWEG *, J. NECHWATAL ,T.JUNG ,D.E.L.COOKE , 4 1 2 2 J. M. DUNCAN ,G.MU¨LLER-STARCK ,C.LANGEBARTELS ,H.SANDERMANN JR and 3 W. OßWALD 1Faculty of Forest Sciences, Section of Forest Genetics, Ludwig-Maximilians-University Munich, Am Hochanger 13, D-85354 Freising, Germany (R. Schubert for correspondence); 2GSF-National Research Center for Environment and Health, Institute of Biochemical Plant Pathology, Ingoldsta¨dter Landstr. 1, D-85764 Neuherberg, Germany; 3Faculty of Forest Sciences, Institute of Forest Botany, Phytopathology, Ludwig-Maximilians- University Munich, Am Hochanger 13, D-85354 Freising, Germany; 4Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK Summary Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the riboso- mal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105 bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria.
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