Photon Detection Unit

PHOTON DETECTION LOW-LIGHT-LEVEL UNIT DETECTION C9692 SERIES

C9692-11 C9692-12 C9692-13

Excitation light source unit

Optical fiber panel Sample holder unit Sample holder unit

C9692-14 C9692-15 C9692-16 Syringe (sold separately)

Dispenser unit

Optical block panel Sample holder unit Microtube unit OVERVIEW FEATURES

The C9692 series Photon Detection Units are photon count- GPhoton counting with high S/N ratio ing units designed to make low-light measurements at sin- Low noise: 50 s-1 (Typ.at 25 °C) gle photon levels without a time-consuming measurement GComputer control setup. All users need to do is prepare the sample the users Built-in USB interface want to measure and a personal computer (PC). Six models are available so the users can select one that best meets GCE marking compliance your application. GOptical fiber (FC type) compatible (C9692-11) When combined with optional modular units the C9692 is GInterlock function (C9692-12/-13/-14/-15) ideal for various types of measurement. The USB interface Automatically closes optical shutter to prevent ex- allows simple plug & play setup when connecting the C9692 cessive light from entering PMT if sample compart- to a PC. ment is accidentally opened during measurement. GBuilt-in UV LED excitation light source (C9692-13) APPLICATIONS Light source wavelength: 375 nm Output power: 10 mW/cm2 GBioluminescence, chemiluminescence Irradiation time: 0.1 to 3600 seconds GFood oxidation, antioxidant activity luminescence GReagent dispensing (C9692-14/-15) G Dispenses two types of reagents using syringe (C9692-14), Activated cell luminescence pipette (C9692-15) as a dispenser GUV-excited (UV LED) delayed fluorescence (C9692-13) GOther low-light-level measurements APPLICATIONS

Application 1 Evaluating compost maturity

Organic farming is the focus of much recent attention, and is a field 6000 where the market value of good quality compost is on the rise. To ensure that good quality compost can be quickly supplied when needed, photon 5000 detection units are used in research to develop techniques for 4000 determining compost maturity during the compost purification process. IMMATURE COMPOST Focusing on the fact that good quality compost is oxidized excrement, 3000 researchers added oxygen to liquid extracted from compost during the MEDIUM MATURE COMPOST fermentation process to accelerate oxidation of residual organic matter and then rated the maturation from the intensity of low-level luminescence 2000 FULLY MATURE COMPOST emitted during the oxidation process. Good quality compost has less unoxidized material and so emits low-level luminescence. 100

Hydrothermal Extract liquid LUMINESCENCE INTENSITY (counts/10s) Compost Measure 0 extraction from compost 0 100 200 300 400 500 600 C9692-12 Oxygen TIME (s) Filtration injection Lumines- Data courtesy of: cence Nobuya Katayama, Shizuoka Prefectural Animal Husbandry Experiment Station Mayuko Iwai, Graduate School for Creation of New Photonics Industries

Application 2 Diagnosing fungus-infected plants

Photon detection units are utilized in research to develop techniques for Results from measuring luminescence intensity detecting the low level luminescence emitted from honey fungus which is on fruited body impossible to observe visually a type of mushroom that acts as a parasite and eats into road-side trees. over elapsed time These techniques will serve as tests to diagnose whether a tree is 400000 infected with fungal filaments (hypha). Samples taken from the bark of the suspect tree are measured using a photon detection unit. 300000 The material within the tree bark being eaten away by the fungus undergoes a temperature change due to fungal action so that the luminescence intensity rises with the passage of time. Utilizing these 200000 changes in low-level luminescence intensity reveals whether there is fungal infection or not. 100000 C9692-12

Tree bark LUMINESCENCE INTENSITY (counts/s) 0 Lumines- 0 30 60 90 cence TIME (min)

Upper: Honey fungus (fruited body: luminescence cannot be observed visually) Left: Image observed on fungus culture under bright conditions Data courtesy of: Right: Image observed on fungus culture under dark conditions (luminescence can be observed visually) Masaru Hiroi, Koriyama Women’s University & College

Application 3 Oxidization on one polished rice grain

(Approximate values) The delayed fluorescence intensity from 90 the surface of a single polished rice grain PHOTON DETECTION UNIT C9692 80 irradiated with UV light for 10 seconds is PHOTO- 70 measured using a photon detection unit MULTIPLIER TUBE that contains an excitation light source MODULE 60 and is operated with dedicated software. 50 This measurement yielded a specific SHUTTER AFTER RICE MILLING 40 value for progressive oxidation after rice LOW LEVEL LIGHT FLUORESCENCE 6 MONTHS AFTER RICE MILLING milling with the 30 C9692-13 EXCITATION passage of time. LIGHT UNIT A9860 20 FLUORESCENCE INTENSITY UV LED 10

SAMPLE 0 HOLDER 0 0.5 1 1.5 2 2.5 3 UNIT SAMPLE A9861 TIME (s) Application 4 Luminescence level in ATP method and changes in koji aspergillus body mass during cultivation of rice koji

Photon detection units were used to measure the luminescence level 12 16 ) in the APT method and changes in koji aspergillus body mass of rice ATP LUMINESCENCE LEVEL KOJI KOJI ASPERGILLUS MASS koji cultivated based on the standard koji making test. 10 12 counts/10s)

Comparing the luminescence level in the APT method with the koji as- 5 8 10

pergillus body mass, they show similar changes up to 32 consecutive × hours of rice koji cultivation. Changes in the rice body mass and enzy- matic activity (alpha-amylase) are major quality indicators of koji as- 6 8 pergillus and mainly end during the logarithmic growth phase, so how both methods related was compared in a range of the culture time 4 from 12 to 32 hours, 4 which is a transition to 2 the stationary phase. C9692-15 MASS (mg/g OF ASPERGILLUS BODY LUMINESCENCE LEVEL ( 0 0 Although the number of 0816 24 32 KOJI data was small, the re- CULTURE TIME (h) sults clearly showed a Data courtesy of: high correlation. Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University

Application 5 Viable bacteria count versus luminescence level in ATP method on fresh produce

Photon detection units are used in research to find techniques using 7 chemiluminescence for quickly and easily measuring the degree of purity in food. 6 About 3 leaves each taken from the outermost layer of several pieces 5 of Boston lettuce were thoroughly crushed and diluted about 10 times

with distilled sterile water for use as the sample fluid concentrate and 4 the luminescence intensity measured by the ATP method using the photon detection unit. 3 The number of viable C9692-15 2 y = 1.33 × -1.52 bacteria cultured by the R2 = 0.80

official analytical method 1 was then found and the VIABLE BACTERIA COUNT (Log of CFU/g) VIABLE BACTERIA 0 correlation with the lumi- 02135746 nescence level found. LUMINESCENCE LEVEL (Log of counts/10s) Data courtesy of: Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University

Application 6 Evaluation of refined sake deterioration

Refined sake (rice wine) oxidizes or in other words degrades after the con- Evaluation by XYZ measurement system tainer or bottle is opened. This oxidation was evaluated with photon detec- 100 90 60 days tion units utilizing the following two methods. 30 days 80 0 days Evaluation by XYZ measurement system (upper graph) 70 The degradation (or oxidation) occurring in refined sake was evaluated by 60 50 ranking refined sake as reactive oxygen species (X) and using a mixture of 40 anti-oxidation species (Y) and receptor species (Z). 30 Evaluation by oxidation reaction measurement system (lower graph) 20 10 The degradation (or oxidation) occurring in refined sake was evaluated us- LUMINESCENCE INTENSITY (count/s) 0 ing a mixture of 75 microliters of hypochlorous natrium to 3 milliliters of re- 0 50 100 150 200 fined sake. TIME (s) Evaluation by oxidation reaction measurement system In both methods the luminescence intensity (degradation level) rose in pro- 15000 portion to the number of days in storage. 60 days 30 days 12000 0 days Left standing C9692-14 in 30 °C 9000 environment 6000

3000

LUMINESCENCE INTENSITY (count/s) 0 0 5 10 15 20 25 30 TIME (s) Data courtesy of: Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University SPECIFICATIONS

C9692 SERIES COMMON SPECIFICATIONS Parameter Description / Value Unit Detection Method Photon counting method — Spectral Response Range 185 to 650 nm Photocathode Size 16 × 18 mm Maximum Count Rate 3 × 106 s-1 Counter Gate Time 0.001 to 10 (1, 2, 5 Steps) s Dark Count (Typ. at 25 °C) 50 s-1 Counter Capacity 32 bits/gate — Trigger Signal Input Mode External trigger, software trigger — Trigger Section Trigger Signal Level TTL negative logic — Trigger Signal Pulse Width 100 ns or more — Input Voltage (DC) +7 (supplied from AC adapter) V Input Voltage (AC) to Supplied AC Adapter 100 V to 240 V (auto switchable), single phase 50 Hz/60 Hz — Temperature +5 to +40 °C Operating Humidity Below 80 (no condensation) % Temperature 0 to +50 °C Storage Humidity Below 85 (no condensation) % OS Windows® XP-Pro, Vista Business (32 bit), 7 Pro (32 bit) — Interface USB — C9692-11 (Optical Fiber Panel Type) Parameter Description / Value Unit Optical Fiber Adapter FC type (HRFC-R1/Hirose) — Distance to Photocathode 21.0 (from fiber end) mm Weight Approx. 0.7 kg C9692-12 (Sample Holder Unit Type) Parameter Description / Value Unit Effective Size of Sample Compartment (W × D × H) 50 × 50 × 15 mm Distance to Photocathode 32.5 (from bottom of sample compartment) mm Weight Approx. 1.1 kg C9692-13 (Sample Holder Unit + Excitation Light Source Unit Type) Parameter Description / Value Unit Wavelength·Output Power 375 nm·10 mW/cm2 — Excitation Light Irradiation Time 0.1 to 3600 s Source (UV LED) Irradiation Area 10 (center of sample compartment) mm Effective Size of Sample Compartment (W × D × H) 50 × 50 × 15 mm Distance to Photocathode 49.5 (from bottom of sample compartment) mm Weight Approx. 1.2 kg C9692-14 (Sample Holder Unit + Dispenser Unit Type) Parameter Description / Value Unit Suitable Syringe Capacity 5 (Terumo syringe) ml Needle Size Outer diameter: 1 mm, inner diameter: 0.6 mm — Recommended Gas Flow Tube Outer diameter: 6 mm (Black tube is recommended for light shielding) — Distance to Photocathode 49.5 mm Weight Approx. 1.2 kg C9692-15 (Microtube Unit Type) Parameter Description / Value Unit Suitable Microtube 500 µl Distance to Photocathode (to microtube surface) 23.0 mm Weight Approx. 1.4 kg C9692-16 (Optical Block Panel Type) Parameter Description / Value Unit Suitable Optical Block V-groove type — Distance to Photocathode 23.3 mm Weight Approx. 0.7 kg ACCESSORIES (Supplied) GCD-ROM (control software) GUSB cable (1.5 m) GAC adapter GCable for external trigger (1.5 m) G1-rack (96 pcs) of pipette tips (C9692-15) CONTROL SOFTWARE FUNCTIONS

GTime-resolved measurement Resolves measurement time per unit time (1 ms or more) allowing measurement of various light emission patterns. GOptical shutter control Opens or closes optical shutter for excess light protection and dark current pulse measurement. GData display during measurement Continuously transfers measurement data to PC for data monitoring. GMeasurement data save Saves measurement data in Excel format to make data analysis easier.

OPTIONAL MODULAR UNITS (available at additional cost)

Optical Fiber Panel A9859Excitation Light Source Unit A9860 Sample Holder Unit A9861

Disperser Unit A10490 Microtube Unit A11044 Pipette Tips A11045

(A11045: 10-rack set) Optical Block Panel A9859-01 SETUP DIAGRAMS

GC9692-11 GC9692-12 Major applications: Various measurements using optical fiber (FC type) Major applications: Bioluminescence, chemiluminescence

DC +7 V IN DC +7 V IN AC ADAPTER AC ADAPTER PHOTON DETECTION UNIT C9692 (SUPPLIED) PHOTON DETECTION UNIT C9692 (SUPPLIED) AC100 V to 240 V AC100 V to 240 V

PHOTO- PHOTO- MULTIPLIER MULTIPLIER TUBE TUBE MODULE MODULE

USB USB SHUTTER SHUTTER

LOW LEVEL LOW LEVEL LIGHT PC LIGHT PC OPTICAL FIBER PANEL A9859 SAMPLE HOLDER UNIT A9861 SAMPLE OPTICAL FIBER (FC TYPE) TPMOC0190EA TPMOC0191EA

GC9692-13 GC9692-14 Major applications: Fluorescence, delayed fluorescence, phosphorescence Major applications: Chemiluminescence

DC +7 V IN DC +7 V IN AC ADAPTER AC ADAPTER PHOTON DETECTION UNIT C9692 (SUPPLIED) PHOTON DETECTION UNIT C9692 (SUPPLIED) AC100 V to 240 V AC100 V to 240 V

PHOTO- PHOTO- MULTIPLIER MULTIPLIER TUBE TUBE MODULE MODULE

USB USB SHUTTER SHUTTER

LOW LEVEL LIGHT LOW LEVEL FLUORESCENCE PC LIGHT PC

EXCITATION LIGHT UNIT DISPENSER UNIT A9860 A10490

UV LED

SAMPLE HOLDER UNIT SAMPLE HOLDER UNIT A9861 A9861 SAMPLE SAMPLE

TPMOC0188EA TPMOC0205EA

GC9692-15 GC9692-16 Major applications: Chemiluminescence Major applications: Measurements with optical blocks

PIPETTE TIP A11045 DC +7 V IN AC ADAPTER PHOTON DETECTION UNIT C9692 DC +7 V IN (SUPPLIED) PHOTON DETECTION UNIT C9692 AC ADAPTER AC100 V to 240 V (SUPPLIED) AC100 V to 240 V PHOTO- SHUTTER PHOTO- MULTIPLIER MULTIPLIER TUBE LOW LEVEL LIGHT TUBE MODULE MODULE MICROTUBE USB USB SHUTTER

PC LOW LEVEL MICROTUBE LIGHT PC UNIT OPTICAL BLOCK PANEL A11044 A9859-01

OPTICAL BLOCK TPMZC0014EA TPMZC0015EA DIMENSIONAL OUTLINES (Unit: mm)

GC9692-11 SIDE VIEWFRONT VIEW SIDE VIEW USB DC IN ON OFF 100 EXT. TRIG PMT POWER ERROR POWER PANEL PHOTON DETECTION UNIT C9692

100 101.5 8.5 42 OPTICAL FIBER ADAPTER

FC TYPE: HRFC-R1 (HIROSE) TPMOA0033EA GC9692-12 TOP VIEW FRONT VIEW SIDE VIEW

PHOTON DETECTION UNIT C9692 EXT. TRIG

PMT POWER USB

ERROR 100

POWER 124.5

OFF

PANEL DC IN

ON 8 15 30 100 50 17 SAMPLE COMPARTMENT 78 TPMOA0034EA

G C9692-13 TOP VIEW FRONT VIEW SIDE VIEW

PHOTON DETECTION UNIT C9692 EXT. TRIG

PMT POWER USB

ERROR 100

POWER 142

OFF

PANEL DC IN ON UV LED

100 8 15 30 18

50 17 SAMPLE COMPARTMENT 78 100 TPMOA0035EA GC9692-14 TOP VIEW FRONT VIEW SIDE VIEW

PHOTON DETECTION UNIT C9692 EXT. TRIG ID SW

PMT POWER USB ERROR SYRINGE 100 POWER INSERTION

142 PORT GAS PORT OFF

PANEL DC IN

ON

100 8 15 30 18

50 17 100±0.5 78 SAMPLE COMPARTMENT

100 TPMOA0045EA DIMENSIONAL OUTLINES (Unit: mm)

GC9692-15 PHOTON DETECTION UNIT C9692

PIPETTE TIP INSERTION PORT

SAMPLE EXCHANGE DOOR

ON

DC IN DC PANEL OFF 2.0

±

POWER ERROR

126.5

USB

PMT POWER PMT

ID SW ID EXT. TRIG EXT.

72 ± 1 12 100 ± 1 160 ± 1 TPMOA0003EA

G C9692-16 SIDE VIEWFRONT VIEW SIDE VIEW USB ID SW DC IN ON OFF 100 EXT. TRIG PMT POWER ERROR POWER PANEL PHOTON DETECTION UNIT C9692

100 96.5 10.8 42 TPMZA0013EA

GSample compartment tray (same for C9692-12, -13, -14)

TOP VIEW 4-R3

56 78 50 69.8 56

17 EFFECTIVE SIZE OF SAMPLE COMPARTMENT 96 (W50 × D50 × H15) TPMOA0034EA / TPMOA0035EA / TPMOA0045EA

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Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office. Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2012 Hamamatsu Photonics K.K.

HAMAMATSU PHOTONICS K.K. www.hamamatsu.com HAMAMATSU PHOTONICS K.K., Electron Tube Division 314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205 U.S.A.: Hamamatsu Corporation: 360 Foothill Road, P. O. Box 6910, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected] Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: [email protected] France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected] United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road Welwyn Garden City Hertfordshire AL7 1BW, United Kingdom, Telephone: 44-(0)1707-294888, Fax: 44(0)1707-325777 E-mail: [email protected] North Europe: Hamamatsu Photonics Norden AB: Smidesvägen 12, SE-171-41 SOLNA, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected] Italy: Hamamatsu Photonics Italia: S.R.L.: Strada della Moia, 1/E, 20020 Arese, (Milano), Italy, Telephone: (39)02-935 81 733, Fax: (39)02-935 81 741 E-mail: [email protected] TPMZ1010E01 China: HAMAMATSU PHOTONICS (CHINA) Co., Ltd.: 1201 Tower B, Jiaming Center, No.27 Dongsanhuan Beilu, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected] JAN. 2012 IP