First Record of the Mediterranean Species Ciboria Brunneorufa in the Balkan Peninsula

CZECH MYCOLOGY 68(2): 127–137, JULY 29, 2016 (ONLINE VERSION, ISSN 1805-1421) First record of the Mediterranean species Ciboria brunneorufa in the Balkan Peninsula NEDIM JUKIĆ Trg Zlatnih Ljiljana 34, BA-71000 Sarajevo, Bosnia and Herzegovina; [email protected] Jukić N. (2016): First record of the Mediterranean species Ciboria brunneorufa in the Balkan Peninsula. – Czech Mycol. 68(2): 127–137. To date, there are no published studies or official papers on mycobiota in coastal Bosnia and Herzegovina. In light of this fact, the author conducted a brief mycological study in the Mediterra- nean part of the country in February 2016. This paper offers a morphological description of the rare Mediterranean species Ciboria brunneorufa, an analysis of microscopic characters and a comparison with data of other authors. It also discusses the global distribution pattern and abundance of C. brunneorufa. An increased level of negative anthropogenic influence is expected on the Klek Peninsula in the future. In order to conserve this habitat unique in Bosnia and Herzegovina and to protect C. brunneorufa and other fungal species, it is necessary to make an environmental risk assessment and to carry out further systematic mycological field studies. Key words: Helotiales, Sclerotiniaceae, Bosnia and Herzegovina, coastal habitat, anthropogenic influence. Article history: received 29 March 2016, revised 26 June 2016, accepted 29 June 2016, published online 29 July 2016. Jukić N. (2016): První nález mediteránního druhu Ciboria brunneorufa na Bal- kánském poloostrově. – Czech Mycol. 68(2): 127–137. Pobřeží Bosny a Hercegoviny je územím, o jehož mykobiotě dosud nebyly publikovány žádné stu- die. Ve světle této skutečnosti provedl autor krátký mykologický průzkum středomořské části země v únoru 2016. Článek předkládá morfologický popis vzácného mediteránního druhu Ciboria brunneorufa, analýzu mikroskopických znaků a srovnání s údaji jiných autorů. Diskutováno je též celkové rozšíření a hojnost výskytu C. brunneorufa. V budoucnu je očekáván nárůst negativního vlivu lidské činnosti na poloostrově Klek. V zájmu ochrany zdejšího biotopu – unikátního v rámci Bosny a Hercegoviny–avzácnýchdruhůhub, jako je právě Ciboria brunneorufa, je nezbytné posouzení environmentálních rizik se zahrnutím dalších systematických terénních průzkumů zdejší mykobioty. INTRODUCTION Although Bosnia and Herzegovina has a very narrow access to the Adriatic Sea (20 km of coastline), its maritime region is part of a distinct geographical area and plays a significant role in terms of Mediterranean biodiversity and its 127 CZECH MYCOLOGY 68(2): 127–137, JULY 29, 2016 (ONLINE VERSION, ISSN 1805-1421) conservation. This small mainland area should be particularly considered an im- portant part of the Mediterranean biodiversity corridor. The vegetation of the Neum-Klek bay is quite similar to that of the surround- ing areas and ecosystems in other Mediterranean countries. Consequently, this area may represent a very interesting habitat in Bosnia and Herzegovina, with a fungal diversity worth investigated. The main feature of the coastal region of Bosnia and Herzegovina is its rather degraded vegetation of the Orno-Quercetum ilicis association, typical of this climate zone (Barudanović et al. 2015), with a predominance of Quercus ilex, Carpinus orientalis, Fraxinus ornus, Laurus nobilis, Juniperus oxycedrus, Erica arborea, Pistacia lentiscus, Pistacia terebinthus and other Mediterranean species. In the past Mediterranean species of fungi were only poorly inventoried in Bosnia, with no published data or official list. Only a couple of recent papers and works address ascomycetes and their distribution in Bosnia and Herzegovina in general. The main content of these notes and publications are preliminary check- lists and taxonomic classifications and descriptions of ecological preferences of certain species (Jukić & Omerović 2011, Usćuplić 2012, Omerović & Jukić 2015). On the other hand, research, inventories and monitoring of certain species of ascomycetes in neighbouring Mediterranean countries have been undertaken more systematically (Matočec 2000, Matočec & Kušan 2008, Van Vooren 2010, Doveri 2011, Kušan et al. 2014, Kušan et al. 2015, Van Vooren et al. 2015a, Van Vooren et al. 2015b, Loizides et al. 2016). In February 2016, the author conducted a brief mycological study in the Neum-Klek bay and a find of the Mediterranean fungus Ciboria brunneorufa is presented in this paper. The presence of other ascomycetes was not recorded at this time of the year. The only previously published notes dealing with the Sclerotiniaceae family in Bosnia and Herzegovina can be found in Palmer et al. (1994), while some re- cords of different species of the Ciboria genus are mentioned in Jukić & Omerović (2011). Ciboria brunneorufa was first described by Bresadola (1903). Saccardo (1906) later cited the same description. More recently, Ormad & García (2007), Ormad et al. (2010) and Pancorbo & Ribes (2010) provided additional information on its morphol- ogy and ecology including a description of relevant microscopic characteristics. In the past, more detailed and comprehensive studies of the Sclerotiniaceae family and Ciboria genus were conducted by Whetzel (1945), Buchwald (1949), Dumont & Korf (1971), Galán et al. (1996), and Holst-Jensen et al. (1997). The collection of Ciboria brunneorufa from the Neum-Klek bay represents the first record of this species in the territory of Bosnia and Herzegovina and the first record from the Balkan Peninsula as well. 128 JUKIĆ N.: FIRST RECORD OF CIBORIA BRUNNEORUFA IN THE BALKAN PENINSULA MATERIAL AND METHODS Fruitbodies of Ciboria brunneorufa were found and collected at a single lo- cality on 11 February 2016. The collection included the substratum in order to ob- tain vital characteristics of the collected material. The material was analysed according to vital taxonomy methods (Baral 1992). The analysis was performed using a Nikon SE type 102 compound light micro- scope with an oil-immersion lens and 1000× magnification. Micro-photographs were taken using a Sony DSC-H2 camera. Ascospores and other microscopic elements were measured using the Piximčtre software (Henriot & Cheype on-line). Measurements and observations were made on multiple preparations. Living cells were first observed in a tap water mount, later IKI – Lugol’s solution (Baral 1987) and Congo red (0.04% aqueous) were used. Afterwards, additional analyses were conducted on herbarium specimens using Melzer’s reagent (Huhtinen 1990). In all media, 20 spores were measured to statistically analyse their characteristics (length, width, Qe). Qe stands for ascospore length/width ratio and is given here as the mean value. The exsiccate has been preserved and adequately stored in a private herbarium (N.J. – Nedim Jukić). Relevant data have been entered into the Amateur My- cological Association’s electronic database (FAMU). RESULTS AND DISCUSSION Ciboria brunneorufa Bres. 1903 Figs. 1–4 D e s c r i p t i o n. Ascomata apothecial, stipitate, 2–4 mm high and 1–3 mm in diam. Hymenium smooth, beige to brown with a pinkish or rust tint (but paler colours are quite dominant during dry weather conditions). Margin conspicuous in mature apothecia, usually slightly darker than hymenium. Outer side of apothecia brighter than hymenium, with fine furfuraceous, sometimes almost hairy surface all over the stipe. Asci cylindrical, eight-spored, 105–135(140) × 8–12.5 μm, emerging from cro- ziers, in IKI euamyloid, type bb (Baral 1987). Ascospores biseriate, in tap water mount (13.4)14–16.8(17.4) × (4.5)5–5.6(5.8) μm, Qe = 2.9; treated with Lugol’s solution (11.2)12.9–15.3(15.5) × (3.3)4.6–5.4(5.5) μm; in Congo red (12.7)13–14.9(15.6) × (4.4)4.7–5.6(6.3) μm; in herbarium material treated with Melzer’s reagent (9.9)11.4–13.1(13.9) × (3.4)3.7–4.2(4.3) μm, Qe = 3.1; smooth and hyaline, aseptate, ellipsoid to fusiform, often slightly inequilat- eral, with 1–4 larger lipid bodies of 0.7–1.5 μm diam. and several smaller ones in 129 CZECH MYCOLOGY 68(2): 127–137, JULY 29, 2016 (ONLINE VERSION, ISSN 1805-1421) each polar half. The number of lipid bodies seemed to be reduced when treated with Congo red. This is probably a consequence of either coalescence of minute lipid bodies with larger ones or minute lipid bodies becoming masked in this medium. Ascospores containing a single central nucleus, 3 μm diam. Presence of a nucleolus, about 1 μm diam., was also recorded. Paraphyses cylindrical, septate, up to 130 μm in length, usually as long as asci. Two types observed: one type rather wide, 3.5–5.5 μm at apex, septate, containing refractive vacuoles with pink-reddish pigment, the other one narrower, 2.2–3.7 μm, septate, non-refractive and hyaline, rarely bifurcate. Medullary excipulum composed of cylindrical elongated, subhyaline to brownish, slightly curved cells with rounded ends forming a textura porrecta, 5.4–9.2 μm wide and 50–110 μm in length. Ectal excipulum consisting of irregularly globose cells, 7–35 μm diam., forming a textura globulosa-subangularis. Towards the external part cells become grad- ually elongated, resembling short hyphoid hairs, 4.5–6 μm wide at the top, containing refractive vacuoles and a pigment similar to those in the paraphyses (Fig. 4c). Comparison of microscopic characters.Somesmallinconsisten- cies and differences are revealed by comparison of data sets from other papers and the present data (Tab. 1). The measurements of ascospores, asci and paraphyses in Ormad et al. (2010) correspond best to the measurements from the Neum-Klek bay collection. Ascal length and description of aseptate paraphyses in Ormad & García (2007) were a result of errors in the description, as stated and corrected afterwards in Ormad et al. (2010). The presence of an interior nucleolus, 1 μm diam., is pointed out in Ormad et al. (2010) as well. The dimensions of the textura porrecta cells in Ormad et al. (2010) are 8–10 × 100–130 μm and are slightly longer and wider than those in the collection from the Neum-Klek bay.

Load more