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Rapid NH System Rapid Spot Indole Reagent (R8309002, Supplied Separately) • When Using the 1-Hour Procedure, Only Selective Notes: (15 Ml/Bottle) Agars Can Be Used
n,n-Dimethyl-1-naphthylamine ..................................... 6.0 g Selective Media: Thayer-Martin Agar; Martin-Lewis 6. Return the panel to a level position. If necessary, gently Glacial Acetic Acid ................................................... 280.0 ml Agar; New York City Agar. tap the panel on the bench top to remove any air Demineralized Water ............................................... 720.0 ml Notes: trapped in the cavities. RapID NH System RapID Spot Indole Reagent (R8309002, supplied separately) • When using the 1-hour procedure, only selective Notes: (15 ml/Bottle) agars can be used. • Examine the test cavities which should appear R8311001 .................................................20 Tests/Kit ρ-Dimethylaminocinnamaldehyde .............................. 10.0 g • Cultures used for inoculum preparation should bubble-free and uniformly filled. Slight irregularities 1. INTENDED USE Hydrochloric Acid .................................................... 100.0 ml preferably be 18-24 hours old. Slow-growing isolates in test cavity fills are acceptable and will not affect Remel RapID™ NH System is a qualitative micromethod Demineralized Water ............................................... 900.0 ml may be tested using 48-hour cultures. test performance. If the panel is grossly misfilled, employing conventional and chromogenic substrates for the *Adjusted as required to meet performance standards. a new panel should be inoculated and the misfilled • The use of media other than those recommended panel discarded. identification of medically important species of Neisseria, 5. PRECAUTIONS may compromise test performance. Haemophilus, and other bacteria isolated from human • Complete the inoculation of each panel receiving in vitro clinical specimens. A complete listing of the organisms This product is for diagnostic use and should be used 3. Using a cotton swab or inoculating loop, suspend inoculation fluid before inoculating additional addressed by RapID NH System is provided in the RapID NH by properly trained individuals. -
Comparison of Three Dispersion Procedures for Quantitative Recovery of Cultivable Species of Subgingival Spirochetes SERGIO L
JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 1987, p. 2230-2232 Vol. 25, No. 11 0095-1137/87/112230-03$02.00/0 Copyright © 1987, American Society for Microbiology Comparison of Three Dispersion Procedures for Quantitative Recovery of Cultivable Species of Subgingival Spirochetes SERGIO L. SALVADOR, SALAM A. SYED, AND WALTER J. LOESCHE* Department of Microbiology and Immunology, University of Michigan School of Dentistry and University of Michigan School of Medicine, Ann Arbor, Michigan 48109-1078 Received 9 April 1987/Accepted 3 August 1987 Spirochetes are usually the predominant organisms observed microscopically in subgingival plaques removed from tooth sites associated with periodQntitis, but these organisms are rarely isolated by cultural means, presumably because the media do not support their growth and/or because these fragile organisms are disrupted by the various procedures used to disperse plaque samples. In the present investigation, three dispersal procedures, sonification, mechanical mixing, and homogenization, were compared for their ability to permit the isolation of Treponema denticola, Treponema vincentii, Treponema socranskii, and Treponema pectinovorum from plaque samples on media that support the growth of these species. Plaque samples in which the spirochetes averaged 50% of the microscopic count were chosen. The highest viable recoveries of spirochetes were observed when the plaques were dispersed with a Tekmar homogenizer, and the lowest occurred with sonification. The highest recoveries averaged only about 1% of the total cultivable counts, indicating either that the sought-after species were minor members of the flora or that the dispersal procedures were still too harsh. A total of 91% of the isolates were T. denticola, 5% were T. -
Identification by 16S Ribosomal RNA Gene Sequencing of Arcobacter
182 ORIGINAL ARTICLE Identification by 16S ribosomal RNA gene sequencing of Mol Path: first published as 10.1136/mp.55.3.182 on 1 June 2002. Downloaded from Arcobacter butzleri bacteraemia in a patient with acute gangrenous appendicitis SKPLau,PCYWoo,JLLTeng, K W Leung, K Y Yuen ............................................................................................................................. J Clin Pathol: Mol Pathol 2002;55:182–185 Aims: To identify a strain of Gram negative facultative anaerobic curved bacillus, concomitantly iso- lated with Escherichia coli and Streptococcus milleri, from the blood culture of a 69 year old woman with acute gangrenous appendicitis. The literature on arcobacter bacteraemia and arcobacter infections associated with appendicitis was reviewed. Methods: The isolate was phenotypically investigated by standard biochemical methods using conventional biochemical tests. Genotypically, the 16S ribosomal RNA (rRNA) gene of the bacterium was amplified by the polymerase chain reaction (PCR) and sequenced. The sequence of the PCR prod- uct was compared with known 16S rRNA gene sequences in the GenBank by multiple sequence align- ment. Literature review was performed by MEDLINE search (1966–2000). Results: The bacterium grew on blood agar, chocolate agar, and MacConkey agar to sizes of 1 mm in diameter after 24 hours of incubation at 37°C in 5% CO2. It grew at 15°C, 25°C, and 37°C; it also grew in a microaerophilic environment, and was cytochrome oxidase positive and motile, typically a member of the genus arcobacter. Furthermore, phenotypic testing showed that the biochemical profile See end of article for of the isolate did not fit into the pattern of any of the known arcobacter species. -
Bacterial Diversity Within the Human Subgingival Crevice
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln U.S. Department of Veterans Affairs Staff Publications U.S. Department of Veterans Affairs 12-7-1999 Bacterial diversity within the human subgingival crevice Ian Kroes Stanford University School of Medicine Paul W. Lepp Stanford University School of Medicine, [email protected] David A. Relman Stanford University School of Medicine, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/veterans Kroes, Ian; Lepp, Paul W.; and Relman, David A., "Bacterial diversity within the human subgingival crevice" (1999). U.S. Department of Veterans Affairs Staff Publications. 18. https://digitalcommons.unl.edu/veterans/18 This Article is brought to you for free and open access by the U.S. Department of Veterans Affairs at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in U.S. Department of Veterans Affairs Staff Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Bacterialdiversity within the human subgingivalcrevice Ian Kroes, Paul W. Lepp, and David A. Relman* Departmentsof Microbiologyand Immunology,and Medicine,Stanford University School of Medicine,Stanford, CA 94305, and VeteransAffairs Palo Alto HealthCare System, Palo Alto,CA 94304 Editedby Stanley Falkow, Stanford University, Stanford, CA, and approvedOctober 15, 1999(received for review August 2, 1999) Molecular, sequence-based environmental surveys of microorgan- associated with disease (9-11). However, a directcomparison isms have revealed a large degree of previously uncharacterized between cultivation-dependentand -independentmethods has diversity. However, nearly all studies of the human endogenous not been described. In this study,we characterizedbacterial bacterial flora have relied on cultivation and biochemical charac- diversitywithin a specimenfrom the humansubgingival crevice terization of the resident organisms. -
Bacterial Communities Associated with Chronic Rhinosinusitis and the Impact of Mucin
Bacterial communities associated with chronic rhinosinusitis and the impact of mucin degradation on Staphylococcus aureus physiology A DISSERTATION SUBMITTED TO THE FACULTY OF THE UNIVERSITY OF MINNESOTA BY Sarah Kathleen Lucas IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY Advisor: Ryan C. Hunter August 2020 © Sarah Kathleen Lucas 2020 Acknowledgements First, this thesis was made possible by the support of the Microbiology, Immunology, and Cancer Biology graduate program, and the faculty and staff of the Department of Microbiology & Immunology at the University of Minnesota. The contribution of the research community and resources available to me at the University of Minnesota cannot be overstated, and I feel very fortunate to have had the opportunity to work within a community that upholds high scholarly standards and are also a pleasure to be around. I would like to extend my deepest gratitude to several individuals for their support during my doctoral training: My advisor, Dr. Ryan Hunter: Thank you for the confidence you had in me, especially in the times when I scarcely believed in myself. Thank you for your patience, and for being the first true mentor I ever had. My labmates: it was a pleasure to conduct research alongside you all. My daily sounding boards for far-out ideas, and encouragement to jump into experiments and just “see what happens”. The members of my thesis committee: Gary Dunny, Mark Herzberg, and Dan Knights. Thanks for all the great feedback in our meetings. Your comments on my work over the years have always been constructive. There would be times in our annual meetings where I would find great motivation in your enthusiasm of the data I presented. -
M0495datasheet-Lot0011212
1X Standard Taq Reaction Buffer: Reaction setup: analyze primers. The final concentration of Hot Start 10 mM Tris-HCl Due to the hot start nature of the enzyme, reactions each primer in a PCR may be 0.05–1 µM, typi- 50 mM KCl can be assembled on the bench at room temperature cally 0.1–0.5 µM. Taq DNA Polymerase 1.5 mM MgCl 2 and transferred to a thermocycler. No separate activa- ++ pH 8.3 @ 25°C tion step is required to release the inhibitor from the 3. Mg and additives: 1-800-632-7799 enzyme. Mg++ concentration of 1.5–2.0 mM is optimal [email protected] Unit Definition: One unit is defined as the amount for most PCR products generated with Hot www.neb.com of enzyme that will incorporate 15 nmol of dNTP Add to a sterile thin-walled PCR tube: Start Taq DNA Polymerase. The final Mg++ M0495S 001121214121 into acid insoluble material in 30 minutes at 75°C. 25 µl 50 µl FInaL concentration in 1X Standard Taq Reaction COMPONENT REacTION REacTION CONCENTRATION Buffer is 1.5 mM. This supports satisfactory Unit Assay Conditions: 1X ThermoPol™ Reaction amplification of most amplicons. However, M0495S Buffer, 200 µM dNTPs including [3H]-dTTP and 10X Standard Taq Reaction Buffer 2.5 µl 5 µl 1X Mg++ can be further optimized in 0.5 or 1.0 mM 200 µg/ml activated Calf Thymus DNA. 200 units 5,000 U/ml Lot: 0011212 10 mM dNTPs 0.5 µl 1 µl 200 µM increments using MgCl2 (sold separately). -
Applied Microbiology VOLUME 60 This Page Intentionally Left Blank ADVANCES in Applied Microbiology
ADVANCES IN Applied Microbiology VOLUME 60 This page intentionally left blank ADVANCES IN Applied Microbiology Edited by ALLEN I. LASKIN Somerset, New Jersey SIMA SARIASLANI Wilmington, Delaware GEOFFREY M. GADD Dundee, United Kingdom VOLUME 60 AMSTERDAM • BOSTON • HEIDELBERG • LONDON NEW YORK • OXFORD • PARIS • SAN DIEGO SAN FRANCISCO • SINGAPORE • SYDNEY • TOKYO Academic Press is an imprint of Elsevier Academic Press is an imprint of Elsevier 525 B Street, Suite 1900, San Diego, California 92101-4495, USA 84 Theobald’s Road, London WC1X 8RR, UK This book is printed on acid-free paper. Copyright ß 2006, Elsevier Inc. All Rights Reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the Publisher. The appearance of the code at the bottom of the first page of a chapter in this book indicates the Publisher’s consent that copies of the chapter may be made for personal or internal use of specific clients. This consent is given on the condition, however, that the copier pay the stated per copy fee through the Copyright Clearance Center, Inc. (www.copyright.com), for copying beyond that permitted by Sections 107 or 108 of the U.S. Copyright Law. This consent does not extend to other kinds of copying, such as copying for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale. Copy fees for pre-2006 chapters are as shown on the title pages. If no fee code appears on the title page, the copy fee is the same as for current chapters. -
11|1111 Technical Tips
Downloaded from genome.cshlp.org on October 3, 2021 - Published by Cold Spring Harbor Laboratory Press 11|1111 Technical Tips PCR has revolutionized nucleic acid vapor barrier on the surface of the aque- Wax-embedded analysis in many scientific disciplines, ous mixture. With this approach, the PCR Reagents including molecular biology, medical di- wax must first be melted and solidified agnostics, population genetics, and fo- on top of the lower aqueous layer prior rensic analysis. (1~ PCR is a highly sensi- to addition of the missing compo- tive technique that can selectively nent(s). In a similar approach, wax is enrich for a specific target from a back- melted and solidified to cover the lower Patricia Blair, ground of nonrelated sequences. How- aqueous layer completely. (7~ The miss- Rama Ramanujam, and ever, if all of the reaction components ing reagent is then layered onto the wax Brent A. Burdick (nucleotides, buffer containing magne- cap, followed by two drops of mineral sium, primers, a thermostable DNA poly- oil. When the temperature of the tubes Pharmacia Biotech, Milwaukee, merase, and sample containing the tar- in the thermocycler exceeds the melting Wisconsin get to be amplified) are mixed at room temperature of the wax, the wax rises to temperature prior to thermocycling, pre- the top of the tube and the upper and mature mispriming events such as non- lower aqueous layers mix by convection. specific annealing of primers to nontar- Another variation of the hot start get nucleic acid sequences and the technique involves drying one of the re- production of primer oligomerization action components in trehalose and em- (primer dimer) artifacts may occur. -
Haemophilus] Haemoglobinophilus As Canicola Haemoglobinophilus Gen
Scotland's Rural College Reclassification of [Haemophilus] haemoglobinophilus as Canicola haemoglobinophilus gen. nov., comb. nov. including Bisgaard taxon 35 Christensen, Henrik; Kuhnert, Peter; Foster, Geoffrey; Bisgaard, Magne Published in: International Journal of Systematic and Evolutionary Microbiology DOI: 10.1099/ijsem.0.004881 First published: 15/07/2021 Document Version Peer reviewed version Link to publication Citation for pulished version (APA): Christensen, H., Kuhnert, P., Foster, G., & Bisgaard, M. (2021). Reclassification of [Haemophilus] haemoglobinophilus as Canicola haemoglobinophilus gen. nov., comb. nov. including Bisgaard taxon 35. International Journal of Systematic and Evolutionary Microbiology, 71(7), [004881]. https://doi.org/10.1099/ijsem.0.004881 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Download date: 27. Sep. 2021 1 Supplemetary material for the paper: 2 Reclassification of [Haemophilus] haemoglobinophilus as Canicola haemoglobinophilus 3 gen. nov., comb. nov. including Bisgaard taxon 35 4 By Henrik Christensen, Peter Kuhnert, Geoff Foster and Magne Bisgaard 5 1 Table S1. -
PCR Tools 2Nd Edition PCR Tools 2Nd Edition | 2009
Novagen® PCR Tools 2nd edition PCR Tools 2nd edition | 2009 Thermostable DNA Polymerases ......... 4 Overview and Enzyme Selection Guide ................................. 4 KOD Hot Start DNA Polymerase ............................................. 6 KOD Hot Start Master Mix ....................................................... 8 KOD Xtreme™ Hot Start DNA Polymerase .............................. 9 KOD XL DNA Polymerase .......................................................11 KOD DNA Polymerase ............................................................12 NovaTaq™ Hot Start DNA Polymerase .................................13 NovaTaq Hot Start Master Mix Kit ........................................13 NovaTaq DNA Polymerase ....................................................14 NovaTaq PCR Master Mix .....................................................14 Taq Antibody ...........................................................................15 10 mM dNTP Mix ...................................................................15 Direct PCR from Blood ........................16 BloodDirect™ PCR Buffer Kits ...............................................16 RT-PCR ....................................................18 One Step RT-PCR Master Mix Kit ........................................18 First Strand cDNA Synthesis Kit ..........................................19 Cover and Inside Photography: Chris Bucher Photography, Dale Chihuly Sculpture located at Indiana University, Medical Sciences Building, Indiana, United States. Prices and availability -
Treponema Amylovorum Sp. Nov., a Saccharolytic Spirochete of Medium Size Isolated from an Advanced Human Periodontal Lesion
Wyss, C; Choi, B K; Schüpbach, P; Guggenheim, B; Göbel, U B. Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int. J. Syst. Bacteriol. 1997, 47(3):842-5. Postprint available at: http://www.zora.unizh.ch University of Zurich Posted at the Zurich Open Repository and Archive, University of Zurich. Zurich Open Repository and Archive http://www.zora.unizh.ch Originally published at: Winterthurerstr. 190 Int. J. Syst. Bacteriol. 1997, 47(3):842-5 CH-8057 Zurich http://www.zora.unizh.ch Year: 1997 Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion Wyss, C; Choi, B K; Schüpbach, P; Guggenheim, B; Göbel, U B Wyss, C; Choi, B K; Schüpbach, P; Guggenheim, B; Göbel, U B. Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int. J. Syst. Bacteriol. 1997, 47(3):842-5. Postprint available at: http://www.zora.unizh.ch Posted at the Zurich Open Repository and Archive, University of Zurich. http://www.zora.unizh.ch Originally published at: Int. J. Syst. Bacteriol. 1997, 47(3):842-5 Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion Abstract A highly motile, medium-size, saccharolytic spirochete was isolated from an advanced human periodontal lesion in medium OMIZ-Pat supplemented with 1% human serum. The growth of this organism is dependent on either glucose, maltose, starch, or glycogen. The cells contain six endoflagella, three per pole, which overlap in the central region of the cell body. -
Actinomyces Naeslundii and Aggregatibacter Aphrophilus Brain Abscess in an Adolescent
Arch Clin Med Case Rep 2019; 3 (6): 409-413 DOI: 10.26502/acmcr.96550112 Case Report Actinomyces Naeslundii and Aggregatibacter Aphrophilus Brain Abscess in an Adolescent Michael Croix1, Christopher Schwarz2, Ryan Breuer3,4, Amanda B. Hassinger3,4, Kunal Chadha5, Mark Daniel Hicar4,6 1Division of Internal Medicine and Pediatrics, University at Buffalo. Buffalo, New York, USA 2Division of Emergency Medicine, University at Buffalo. Buffalo, New York, USA 3Division of Pediatric Critical Care, John R. Oishei Children’s Hospital. Buffalo, New York, USA 4Department of Pediatrics, University at Buffalo. Buffalo, New York, USA 5Division of Pediatric Emergency Medicine, University at Buffalo. Buffalo, New York, USA 6Division of Pediatric Infectious Diseases, University at Buffalo. Buffalo, New York, USA *Corresponding Authors: Dr. Mark Daniel Hicar, Department of Pediatrics, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, 1001 Main Street, Buffalo, NY, 14203 USA, Tel: (716) 323-0150; Fax: (716) 888-3804; E-mail: [email protected] (or) [email protected] Dr. Michael Croix, Division of Internal Medicine and Pediatrics, 300 Linwood Ave, Buffalo, NY, 14209 USA, Tel: (217) 840-5750; Fax: (716) 888-3804; E-mail: [email protected] Received: 20 July 2019; Accepted: 02 August 2019; Published: 04 November 2019 Abstract We report the case of a child with a brain abscess from which Actinomyces naeslundii and Aggregatibacter aphrophilus were isolated. The is the first case describing A. naeslundii causing a brain abscess. This case highlights the association of these two organisms which may affect antibiotic choice and therapy length. Keywords: Brain; Abscess; Actinomyces; Aggregatibacter 1. Case Report A 13 year old male with no known past medical history initially presented to the Emergency Department with one week of headache, nausea, and vomiting.