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PCR Tools 2Nd Edition PCR Tools 2Nd Edition | 2009 Novagen® PCR Tools 2nd edition PCR Tools 2nd edition | 2009 Thermostable DNA Polymerases ......... 4 Overview and Enzyme Selection Guide ................................. 4 KOD Hot Start DNA Polymerase ............................................. 6 KOD Hot Start Master Mix ....................................................... 8 KOD Xtreme™ Hot Start DNA Polymerase .............................. 9 KOD XL DNA Polymerase .......................................................11 KOD DNA Polymerase ............................................................12 NovaTaq™ Hot Start DNA Polymerase .................................13 NovaTaq Hot Start Master Mix Kit ........................................13 NovaTaq DNA Polymerase ....................................................14 NovaTaq PCR Master Mix .....................................................14 Taq Antibody ...........................................................................15 10 mM dNTP Mix ...................................................................15 Direct PCR from Blood ........................16 BloodDirect™ PCR Buffer Kits ...............................................16 RT-PCR ....................................................18 One Step RT-PCR Master Mix Kit ........................................18 First Strand cDNA Synthesis Kit ..........................................19 Cover and Inside Photography: Chris Bucher Photography, Dale Chihuly Sculpture located at Indiana University, Medical Sciences Building, Indiana, United States. Prices and availability are subject to change. Copyright © 2009 EMD Chemicals Inc., an Mix Kit, and Taq Antibody. These products are manufactured by Toyobo and distributed affiliate of Merck KGaA, Darmstadt, Germany. All rights reserved. Each product is sold by EMD Chemicals, Inc. Patents related to these products include U.S. Patents 4,965,188, with a limited warranty which is provided with each purchase. Each product is intended 4,889,818, 5,079,352,5,075,216, 5,407,800, 5,322,770, 5,310,652, 5,436,149, 5,338,671, to be used for research purposes only. It is not to be used for drug or diagnostic purposes USSN 07/873, 897,USSN 08/384,490, and European Patent 592,035. nor is it intended for human use. EMD Chemicals products may not be resold, modified for resale, or used to manufacture commercial products without written approval of EMD KOD Hot Start DNA Polymerase, KOD XL DNA Polymerase, KOD Xtreme™ Hot Start DNA Chemicals. BacVector®, His•Tag®, HSV•Tag®, Novagen®, Pellet Paint®, Perfectly Blunt®, Polymerase, NovaTaq™ DNA Polymerase, NovaTaq Hot Start DNA Polymerase, One Step RT- and T7Select® are registered trademarks of EMD Chemicals Inc. in the United States and PCR Master Mix Kit. Use of these products is covered by one of more of the following U.S. in certain other jurisdictions. AccepTor™, BloodDirect™, Clonables™, CytoBuster™, D- Patents and corresponding patent claims outside the U.S.: 5,079,352, 5,618,711, 5,789,224, Tube™, GelMelt™, GigaSingles, HT96™, KOD Xtreme™, NovaTaq™, Perfect DNA™, pETBlue™, 6,127,155 and claims outside the U.S. corresponding to U.S. Patent 4,889,818. The S•Tag™, Singles™, SpinPrep™, Straight A’s™, Tuner™, Veggie™, and Zappers™ are trade- purchase of these products includes a limited, non-transferable immunity from suit under marks of EMD Chemicals Inc. ABI PRISM®, Big Dye®, and MicroAmp® are registered trade- the foregoing patent claims for using only this amount of product for the purchaser’s own marks of Applera Corporation. Cy5® is a registered trademark of GE Healthcare. Ex Taq™, internal research. No right under any other patent claim (such as the patented 5’ Nuclease LA Taq™, and PrimeSTAR® are trademarks of Takara Bio Inc. Herculase®, PfuTurbo®, and Process claims in U.S. Patents 5,210,015 and 5,487,972), no right to perform any patented PfuUltra® are registered trademarks of Stratagene. Platinum®, Pfx50™, and Quant-iT™ are method, and no right to perform commercial services of any kind, including without limi- trademarks of Invitrogen Corp. Norit® is a registered trademark of Norit N.V. Phusion™ is a tation reporting the results of purchaser’s activities for a fee or other commercial consid- trademark of Finnzymes Oy. PicoGreen® and SYBR® are registered trademarks of Molecular eration, is conveyed expressly, by implication, or by estoppel. This product is for research Probes, Inc. use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Patent & Licensing Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404. AccepTor™ Vectors are covered under U.S. Patent 5,856,144 issued to EMD Chemicals Inc. for a vector, method, and kit for direct cloning of PCR products. Pellet Paint® Co-Precipitant is covered under U.S. Patent 7,144,713 and European Patent 0,853,680 issued to EMD Chemicals Inc. for a method for precipitating nucleic acid with D-Tube™ Dialyzers are covered under U.S. Patent 7,074,313, European Patent 1,285,257, a visible carrier. Australian Patent 2001,262,612, Canadian Patent 2,410,322, Indian Patent 201,731, and Israeli Patent 152,986 assigned to Gene Bio-Application Ltd. pETBlue™ Perfectly Blunt® Cloning Kits are licensed under U.S. Patent 5,693,489. For aca- demic or non-profit laboratories, a nondistribution agreement accompanies the products. KOD DNA Polymerase, KOD Hot Start DNA Polymerase, KOD XL DNA Polymerase, KOD Commercial laboratories must obtain a research-use license from Brookhaven Science Xtreme™ Hot Start DNA Polymerase, KOD Hot Start Master Mix, One Step RT-PCR Master Associates prior to purchase of the products. Novagen • PCR Tools RESOURCE GUIDE ........................20 PCR Clean Up and Nucleic Acid Preparation ...................36 Clonables 2X Ligation Premix ...............................................49 SpinPrep™ PCR Clean-Up Kit ................................................36 NovaBlue Competent Cell Formats .......................................50 SpinPrep Gel DNA Kit ............................................................37 NovaBlue GigaSingles™ Competent Cells ............................50 Pellet Paint® Co-Precipitant ..................................................38 NovaBlue Singles™ Competent Cells .................................... 51 Pellet Paint NF Co-Precipitant ..............................................39 NovaBlue T1R Singles Competent Cells ............................... 51 Veggie™ NovaBlue Singles Competent Cells .......................52 Molecular Size Markers ......................40 Zappers™ Electrocompetent Cells .........................................52 Perfect DNA™ Markers ...........................................................40 HT96™ NovaBlue Competent Cells .......................................53 Perfect DNA Ladders .............................................................. 41 HT96 Isothermal Block ..........................................................53 PCR Markers ............................................................................ 41 Molecular Biology Essentials ............54 PCR Cloning ...........................................42 Molecular Biology Reagents ...................................................54 Cloning Kits Overview ............................................................42 Molecular Biology Grade Buffers ..........................................55 AccepTor™ Vector Kits .............................................................44 Molecular Biology Enzymes ...................................................56 Perfectly Blunt® Cloning Kits ................................................46 D-Tube™ Dialyzers ...................................................................58 Clonables™ Ligation/Transformation Kit ..............................49 D-Tube Electroelution Accessory Kit .....................................59 Resource Guide Look for these 20 FAQ icons on the 22 Protocol Comparison 23 Comparing the Speed and Product Yield of 7 High product pages: Fidelity DNA Polymerases S E D N 27 Reliable and Efficient PCR from Whole Blood and E F O C I R Indicates that the enzyme L Crude Tissue Lysates Using the KOD Xtreme™ Hot is licensed for PCR. Start DNA Polymerase System P C R 30 Reliable and Robust Real-time Amplification Using N T P d s NovaTaq™ Hot Start Master Mix Indicates that dNTPs I N are included. 32 One Step Real-Time Amplification of mRNA Using C D L U D E the One Step RT-PCR Master Mix Kit 35 Detection of Shiga toxin-producing E. coli using ON THE W Find more information E B multiplex colony-direct PCR with KOD XL DNA on our website. www.novagen.com Polymerase [email protected] 3 [email protected] Visit our website www.merckbio.com PCR • Thermostable DNA Polymerases Thermostable DNA Polymerases DNA Thermostable Overview and Enzyme Selection Guide A complete selection of enzymes and kits for PCR In the 25 years following the invention of PCR by Dr. Kary KOD Xtreme polymerase provides high accuracy, specificity, Mullis, PCR has evolved to become an integral laboratory and robust yield. tool. High quality thermostable polymerases are critical for consistent performance. We offer a complete selection of Not all applications require high-fidelity, high-performance high-quality Novagen enzymes and kits for a variety of PCR enzymes, but quality is still important. NovaTaq™ DNA applications. Polymerase is a high-purity, recombinant enzyme suitable for any
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