DOCSLIB.ORG

Energy Metabolism in Human Erythrocytes: I. EFFECTS of SODIUM FLUORIDE

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Energy Metabolism in Human Erythrocytes: I. EFFECTS of SODIUM FLUORIDE Energy Metabolism in Human Erythrocytes: I. EFFECTS OF SODIUM FLUORIDE Stephen A. Feig, … , Stephen B. Shohet, David G. Nathan J Clin Invest. 1971;50(8):1731-1737. https://doi.org/10.1172/JCI106662. Exposure of red cells to fluoride produces a variety of metabolic alterations, most of which are based upon the secondary effects of enolase inhibition, which reduces pyruvate synthesis and interferes with the regeneration of diphosphopyridine nucleotide (NAD). Adenosine triphosphate (ATP) is consumed in the hexokinase and phosphofructokinase reactions but is not regenerated since the deficiency of NAD limits glyceraldehyde phosphate dehydrogenase. ATP depletion in the presence of fluoride and calcium induces a massive loss of cations and water. Of the other known sites of ATP utilization, membrane-bound ATPase is inhibited by fluoride, but the incorporation of fatty acids into membrane phospholipids is unaffected until ATP is depleted. The addition of methylene blue to fluoride-treated red cells regenerates NAD, permitting triose oxidation and the generation of 3-phosphoglycerate and 2,3-diphosphoglycerate. Enolase inhibition is then partially overcome by mass action, and sufficient glycolysis proceeds to maintain the concentration of ATP. This in turn prevents the massive cation and water loss, and permits membrane phospholipid renewal to proceed. Membrane ATPase activity is not restored by the oxidant so that normal cation leakage remains unopposed by cation pumping in red cells exposed to the combination of fluoride and methylene blue. Find the latest version: https://jci.me/106662/pdf Energy Metabolism in Human Erythrocytes I. EFFECTS OF SODIUM FLUORIDE STEPHEN A. FEIG, STEPHEN B. SHOHET, and DAvm G. NATHAN From the Division of Hematology of the Department of Medicine, Children's Hospital Medical Center, and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115 A B S T R A C T Exposure of red cells to fluoride pro- model system with particular reference to inherited duces a variety of metabolic alterations, most of which erythrocyte pyruvate kinase deficiency (2). This paper are based upon the secondary effects of enolase inhibi- presents an analysis of the metabolic perturbations pro- tion, which reduces pyruvate synthesis and interferes duced by fluoride in the red cell and also describes their with the regeneration of diphosphopyridine nucleotide partial correction by the introduction of an oxidant, (NAD). Adenosine triphosphate (ATP) is consumed methylene blue. in the hexokinase and phosphofructokinase reactions but is not regenerated since the deficiency of NAD limits METHODS glyceraldehyde phosphate dehydrogenase. ATP deple- Human blood samples were obtained from nonfasting nor- tion in the presence of fluoride and calcium induces a mal subjects and patients with young red cell populations. massive loss of cations and water. These included patients recovering from iron deficiency, Of the other known sites of ATP utilization, mem- pernicious anemia, and acute hemorrhage, as well as a pa- ATPase is inhibited by fluoride, but the tient with an unstable hemoglobin hemolytic anemia and brane-bound another with an acquired hemolytic anemia. They were col- incorporation of fatty acids into membrane phospho- lected in preservative-free heparin (0.1 mg/ml) and washed lipids is unaffected until ATP is depleted. three times at 4VC in Krebs-Henseleit buffer containing al- The addition of methylene blue to fluoride-treated bumin (1 g/100 ml). The final hematocrit was adjusted to red cells regenerates NAD, permitting triose oxidation approximately 30%7 with the same buffer. Measurements of packed cell volume, red cell count, hemoglobin concentra- and the generation of 3-phosphoglycerate and 2,3-di- tion, and reticulocyte count were performed by standard phosphoglycerate. Enolase inhibition is then partially methods (3) and in a Coulter model S counter. Glutamic overcome by mass action, and sufficient glycolysis pro- oxaloacetic transaminase activity of hemolysates was per- ceeds to maintain the concentration of ATP. This in formed by the method of Karmen, Wroblewski, and LaDue turn prevents the massive cation and water loss, and (4). Cell suspensions (3-8 ml in 25-ml Erlenmeyer flasks) permits membrane phospholipid renewal to proceed. were gassed with a mixture of air and 5% C02 to bring the Membrane ATPase activity is not restored by the oxi- pH to 7.5 +0.1. Additives were prepared to provide desired dant so that normal cation leakage remains unopposed final concentrations by the addition of 10 ul to the incubation by cation pumping in red cells exposed to the combina- flask. Incubations were carried out in a 37'C water bath blue. at 60 oscillations/min. NaH2P04 was added to give a final tion of fluoride and methylene concentration of 10 mmoles/liter of incubation mixture. The pH was measured when samples were obtained. If the pH had changed by more than 0.1 U, the samples were gassed INTRODUCTION with 5% CO2 or left open briefly to bring the pH back to In the search for improved methods of management of 7.5. Filtrates were prepared at 4°C in two volumes of 1 N anemia, the cor- perchloric acid before and during the incubations. These patients with congenital hemolytic were centrifuged at 4'C twice to remove the sediment, neu- rection of induced disorders of erythrocyte metabolism tralized with 5 M K2C0, and frozen within 2 hr. These may be instructive. The inhibition of glycolysis at the were later analyzed for ATP (5), glucose (6), lactate (7), level of enolase by fluoride (1) has been used as a and 2,3-diphosphoglycerate (2,3-DPG) (8) .1 Dr. Shohet is a Fellow of the Medical Foundation Inc. 1Abbreviationis used in this paper: DHAP, dihydroxy ace- Dr. Nathan is the recipient of U. S. Public Health Service' tone phosphate; 2,3-DPG, 2,3-diphosphoglycerate; FDP, Research Career Development Award K3-AM 35361. fructose diphosphate; G3P, glyceraldehyde-3-phosphate; Received for publication 25 November 1970 and in revised GPD, glyceraldehyde-3-phosphate dehydrogenase; 2PG, 2- form 24 February 1971. phosphoglycerate; 3PG, 3-phosphoglyceric acid. The Journal of Clinical Investigation Volume 50 1971 1731 Other glycolytic intermediates in the filtrates were mea- had no discernible effect on ATP and 2,3-DPG concen- sured in assays of 1 ml final volume by modifications of trations when used alone, and NaCN increased lactate the fluorometric methods of Lowry, Passonneau, Hassel- less than The rate berger, and Schulz (9) with an Eppendorf Fluorimeter, production in immature cells 10%. model 1100 M. For hexose monophosphates, 0.1-0.4 ml of of ATP decline seemed faster in immature cells, but the neutralized filtrate was used in a system containing 100 mat difference was not statistically significant. Stable levels Tris buffer (pH 8.0), 80 AM NADP, and 10 mM MgCl2. The were attained in both in less than 1 hr. No further de- increase in fluorescence was always less than that produced cline was noted at 3 or 4 hr. The final ATP concentra- by a standard of 4 mptmoles of NADPH. For fructose di- phosphate (FDP), dihyroxy acetone phosphate (DHAP), tion, 0.49 +0.13 mmoles/liter cells, was approximately and glyceraldehyde-3-phosphate (G3P), 0.05-0.2 ml of neu- one-third of the initial value. tralized filtrate was used in a system containing 16 mm imid- Fig. 1 also shows the rate of loss of potassium from azol buffer (pH 7.0) and 4-16 /LM NADH. For 3-phospho- fluoride-treated cells. After a lag period, during which glyceric acid (3 PG), 0.2 ml of neutralized filtrate was used ensued, especially in a system containing 16 mar imidazol buffer (pH 7.0), 5 ATP fell, a dramatic loss of potassium mM 2-mercaptoethanol, 5 mat MgC12, 0.3 mm ATP, and 4 in immature cells. We also confirmed earlier findings /Lm NADH. For pyruvate, perchloric acid filtrates were (16-19) that this massive effect on potassium perme- prepared from buffer, the red cells having been eliminated ability was inhibited by 5 mM EDTA and was not ob- by centrifugation (10). 0.05-0.2 ml of a neutralized filtrate free buffer (data not shown). In spite of this protec- was used in a system containing a final concentration of 40 mm phosphate buffer (pH 7.0), 4 mm MgCl2 and 4-16 tion against potassium loss, ATP stability was unaf- ILM NADH. With the methodology used, fluoride interfered served when the incubation was performed in a calcium- with the accurate measurement of 2-phosphoglycerate fected, confirming previous observations (15). The ad- (2PG), and this intermediate was not estimated. Neither dition of 2 mM ATP to the incubation mixture did not fluoride nor methylene blue interfered with the other en- protect fluoride-treated cells against potassium loss. zymatic determinations. The release of potassium from incubated red cells was estimated by the change in the po- Since the effect of fluoride is known to be dependent tassium ion concentration in the incubation medium measured upon phosphate concentration (20), cells were incu- with an internally standardized flame photometer (model bated in 10 mm sodium fluorophosphate to determine 143; Instrumentation Laboratory Inc., Watertown, Mass.). whether that complex might be directly involved in the All of the above concentrations were expressed per liter of red cells based upon the initial hematocrit of the cell sus- massive potassium loss. Neither potassium loss nor in- pension. hibition of lactate production were observed under The activity of membrane-bound ATPase (11) was mea- these conditions.2 sured in ghosts prepared by the Nakao, Kurashina, and Na- kao (12) modification of the method of Dodge, Mitchell, The effect of fluoride on ATP metabolism and Hanahan (13). The incorporation of radioactive fatty acid into red The dramatic loss of red cell ATP encouraged an cell membrane phospholipid was also measured.
Load more

Recommended publications
  • Eradication of ENO1-Deleted Glioblastoma Through Collateral Lethality
    bioRxiv preprint doi: https://doi.org/10.1101/331538; this version posted May 25, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Eradication of ENO1-deleted Glioblastoma through Collateral Lethality Yu-Hsi Lin1, Nikunj Satani1,2, Naima Hammoudi1, Jeffrey J. Ackroyd1, Sunada Khadka1, Victoria C. Yan1, Dimitra K. Georgiou1, Yuting Sun3, Rafal Zielinski4, Theresa Tran1, Susana Castro Pando1, Xiaobo Wang1, David Maxwell5, Zhenghong Peng6, Federica Pisaneschi1, Pijus Mandal7, Paul G. Leonard8, Quanyu Xu,9 Qi Wu9, Yongying Jiang9, Barbara Czako10, Zhijun Kang10, John M. Asara11, Waldemar Priebe4, William Bornmann12, Joseph R. Marszalek3, Ronald A. DePinho13 and Florian L. Muller#1 1) Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX 77054 2) Institute of Stroke and Cerebrovascular Disease, The University of Texas Health Science Center at Houston, TX 77030 3) Center for Co-Clinical Trials, The University of Texas MD Anderson Cancer Center, Houston, TX 77054 4) Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX 77054 5) Institutional Analytics & Informatics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030 6) Cardtronics, Inc., Houston, TX 77042 7) Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77054 bioRxiv preprint doi: https://doi.org/10.1101/331538; this version posted May 25, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
    [Show full text]
  • Structures, Functions, and Mechanisms of Filament Forming Enzymes: a Renaissance of Enzyme Filamentation
    Structures, Functions, and Mechanisms of Filament Forming Enzymes: A Renaissance of Enzyme Filamentation A Review By Chad K. Park & Nancy C. Horton Department of Molecular and Cellular Biology University of Arizona Tucson, AZ 85721 N. C. Horton ([email protected], ORCID: 0000-0003-2710-8284) C. K. Park ([email protected], ORCID: 0000-0003-1089-9091) Keywords: Enzyme, Regulation, DNA binding, Nuclease, Run-On Oligomerization, self-association 1 Abstract Filament formation by non-cytoskeletal enzymes has been known for decades, yet only relatively recently has its wide-spread role in enzyme regulation and biology come to be appreciated. This comprehensive review summarizes what is known for each enzyme confirmed to form filamentous structures in vitro, and for the many that are known only to form large self-assemblies within cells. For some enzymes, studies describing both the in vitro filamentous structures and cellular self-assembly formation are also known and described. Special attention is paid to the detailed structures of each type of enzyme filament, as well as the roles the structures play in enzyme regulation and in biology. Where it is known or hypothesized, the advantages conferred by enzyme filamentation are reviewed. Finally, the similarities, differences, and comparison to the SgrAI system are also highlighted. 2 Contents INTRODUCTION…………………………………………………………..4 STRUCTURALLY CHARACTERIZED ENZYME FILAMENTS…….5 Acetyl CoA Carboxylase (ACC)……………………………………………………………………5 Phosphofructokinase (PFK)……………………………………………………………………….6
    [Show full text]
  • Carbon Partitioning in Green Algae (Chlorophyta) and the Enolase Enzyme
    Metabolites 2014, 4, 612-628; doi:10.3390/metabo403000x OPEN ACCESS metabolites ISSN 2218-1989 www.mdpi.com/journal/metabolites/ Article Carbon Partitioning in Green Algae (Chlorophyta) and the Enolase Enzyme Jürgen E. W. Polle 1,2,*, Peter Neofotis 1,2, Andy Huang 1, William Chang 1, Kiran Sury 1 and Eliza M. Wiech 1 1 Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA; E-Mails: [email protected] (P.N.); [email protected] (A.H.); [email protected] (W.C.); [email protected] (K.S.); [email protected] (E.M.W.) 2 The Graduate Center of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +1-718-951-5000; Fax: +1-718-951-4659. Received: 13 June 2014; in revised form: 25 July 2014 / Accepted: 28 July 2014 / Published: 4 August 2014 Abstract: The exact mechanisms underlying the distribution of fixed carbon within photoautotrophic cells, also referred to as carbon partitioning, and the subcellular localization of many enzymes involved in carbon metabolism are still unknown. In contrast to the majority of investigated green algae, higher plants have multiple isoforms of the glycolytic enolase enzyme, which are differentially regulated in higher plants. Here we report on the number of gene copies coding for the enolase in several genomes of species spanning the major classes of green algae. Our genomic analysis of several green algae revealed the presence of only one gene coding for a glycolytic enolase [EC 4.2.1.11].
    [Show full text]
  • Functional and Physiological Discovery in the Mannonate Dehydratase Subgroup of the Enolase Superfamily
    FUNCTIONAL AND PHYSIOLOGICAL DISCOVERY IN THE MANNONATE DEHYDRATASE SUBGROUP OF THE ENOLASE SUPERFAMILY BY DANIEL JOSEPH WICHELECKI DISSERTATION Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biochemistry in the Graduate College of the University of Illinois at Urbana-Champaign, 2014 Urbana, Illinois Doctoral Committee: Professor John Gerlt, Chair Professor John Cronan Professor Scott Silverman Professor Wilfred van der Donk ABSTRACT In the current post-genomic world, the exponential amassing of protein sequences is overwhelming the scientific community’s ability to experimentally assign each protein’s function. The use of automated, homology-based annotations has allowed a reprieve from this efflux of data, but has led to widespread misannotation and nonannotation in protein sequence databases. This dissertation details the functional and physiological characterization of the mannonate dehydratase subgroup (ManD) of the enolase superfamily (ENS). The outcome affirms the dangers of homology-based annotations while discovering novel metabolic pathways. Furthermore, the experimental verification of these pathways ( in vitro and in vivo ) has provided a platform to test the general strategies for improved functional and metabolic characterization being developed by the Enzyme Function Initiative (EFI). Prior to this study, one member of the ManD subgroup had been characterized and was shown to dehydrate D-mannonate to 2-keto-3-deoxy-D-gluconate. Forty-two additional members of the ManD, selected from across the sequence space of the subgroup, were screened for activity and kinetic constants were determined. The members of the once isofunctional subgroup were found to differ in both catalytic efficiency and substrate specificity: 1) high 3 4 -1 -1 efficiency (k cat /K M = 10 to 10 M s ) dehydration of D-mannonate, 2) low efficiency (k cat /K M = 10 1 to 10 2 M-1s-1) dehydration of D-mannonate and/or D-gluconate, and 3) no-activity with either D-mannonate or D-gluconate (or any other acid sugar tested).
    [Show full text]
  • Name: Chem 465 Biochemistry II Test 1 Spring 2019 Multiple Choice (4 Points Apiece): 1. in an Anaerobic Muscle Preparation, Lact
    Name: Chem 465 Biochemistry II Test 1 Spring 2019 Multiple choice (4 points apiece): 1. In an anaerobic muscle preparation, lactate formed from glucose labeled in C-3 and C-4 would be labeled in: A) all three carbon atoms. B) only the carbon atom carrying the OH. C) only the carboxyl carbon atom. D) only the methyl carbon atom. E) the methyl and carboxyl carbon atoms. 2. In an anaerobic muscle preparation, lactate formed from glucose labeled in C-2 would be labeled in: A) all three carbon atoms. B) only the carbon atom carrying the OH. C) only the carboxyl carbon atom. D) only the methyl carbon atom. E) the methyl and carboxyl carbon atoms. 3. All of the following enzymes involved in the flow of carbon from glucose to lactate (glycolysis) are also involved in the reversal of this flow (gluconeogenesis) except: A) 3-phosphoglycerate kinase. B) aldolase. C) enolase. D) phosphofructokinase-1. E) phosphoglucoisomerase. 4. Cellular isozymes of pyruvate kinase are allosterically inhibited by: A) high concentrations of AMP. B) high concentrations of ATP. C) high concentrations of citrate. D) low concentrations of acetyl-CoA. E) low concentrations of ATP. 5. Which of the following is not true of the reaction catalyzed by the pyruvate dehydrogenase complex? A) Biotin participates in the decarboxylation. B) Both NAD+ and a flavin nucleotide act as electron carriers. C) The reaction occurs in the mitochondrial matrix. D) The substrate is held by the lipoyl-lysine "swinging arm." E) Two different cofactors containing -SH groups participate. 6. (20 points) This page is blank because I want you to fill it in with the glycolytic pathway from glucose to pyruvate showing the structure of all intermediates.
    [Show full text]
  • Self-Association of Enolase from Trichomonas Vaginalis. Monomers
    ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. To access the final edited and published work is available online at: http://dx.doi.org/10.1021/acsomega.8b02197 This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Article Cite This: ACS Omega 2018, 3, 17871−17880 http://pubs.acs.org/journal/acsodf Self-Association of Enolase from Trichomonas vaginalis. Monomers, Dimers, and Octamers Coexist in Solution Elibeth Mirasol-Melendez,́ † Enrique Lima,‡ Victor Lara,§ Luis G. Brieba,∥ Samuel Lara-Gonzalez,́ ⊥ and Claudia G. Benitez-Cardoza*,† † Laboratorio de Investigacioń Bioquímica, Escuela Nacional de Medicina y Homeopatía-Instituto Politecnicó Nacional, Guillermo Massieu Helguera No. 239, La Escalera Ticoman, CP 07320 Ciudad de Mexico, Mexico ‡ Instituto de Investigaciones en Materiales, Universidad Nacional Autonomá de Mexico, Circuito exterior s/n, Cd. Universitaria, Del. Coyoacan,́ CP 04510 Ciudad de Mexico, Mexico § Universidad Autonomá Metropolitana, Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, 09340 Ciudad de Mexico, Mexico ∥ Laboratorio Nacional de Genomicá para la Biodiversidad, Centro de Investigacioń y de Estudios Avanzados del IPN, Apartado Postal 629, Irapuato, CP 36821 Guanajuato, Mexico ⊥ Divisioń de Biología Molecular, IPICYT, Camino a la Presa San José2055, CP 78216 San Luis Potosí, San Luis Potosí, Mexico *S Supporting Information ABSTRACT: We used small-angle X-ray scattering to study the self-association of enolase from Trichomonas vaginalis as a function of the protein concentration and cosolute type.
    [Show full text]
  • Enolase 1 Regulates Stem Cell-Like Properties in Gastric Cancer Cells by Stimulating Glycolysis
    Yang et al. Cell Death and Disease (2020) 11:870 https://doi.org/10.1038/s41419-020-03087-4 Cell Death & Disease ARTICLE Open Access Enolase 1 regulates stem cell-like properties in gastric cancer cells by stimulating glycolysis Ting Yang1, Xiong Shu2,Hui-WenZhang1,Li-XinSun1,LongYu1,JunLiu1, Li-Chao Sun1,Zhi-HuaYang1 and Yu-Liang Ran1 Abstract Recent studies have demonstrated that gastric cancer stem cells (CSCs) are a rare sub-group of gastric cancer (GC) cells and have an important role in promoting the tumor growth and progression of GC. In the present study, we demonstrated that the glycolytic enzyme Enolase 1 (ENO1) was involved in the regulation of the stem cell-like characteristics of GC cells, as compared to the parental cell lines PAMC-82 and SNU16, the expression of ENO1 in spheroids markedly increased. We then observed that ENO1 could enhance stem cell-like characteristics, including self- renewal capacity, cell invasion and migration, chemoresistance, and even the tumorigenicity of GC cells. ENO1 is known as an enzyme that is involved in glycolysis, but our results showed that ENO1 could markedly promote the glycolytic activity of cells. Furthermore, inhibiting glycolysis activity using 2-deoxy-D-glucose treatment significantly reduced the stemness of GC cells. Therefore, ENO1 could improve the stemness of CSCs by enhancing the cells’ glycolysis. Subsequently, to further confirm our results, we found that the inhibition of ENO1 using AP-III-a4 (ENOblock) could reduce the stemness of GC cells to a similar extent as the knockdown of ENO1 by shRNA. Finally, increased expression of ENO1 was related to poor prognosis in GC patients.
    [Show full text]
  • Cysteine As a Carbon Source, a Hot Spot in Cancer Cells Survival
    MINI REVIEW published: 23 June 2020 doi: 10.3389/fonc.2020.00947 Cysteine as a Carbon Source, a Hot Spot in Cancer Cells Survival Jacinta Serpa 1,2* 1 CEDOC, Chronic Diseases Research Centre, NOVA Medical School - Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisbon, Portugal, 2 Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG), Lisbon, Portugal Cancer cells undergo a metabolic rewiring in order to fulfill the energy and biomass requirements. Cysteine is a pivotal organic compound that contributes for cancer metabolic remodeling at three different levels: (1) in redox control, free or as a component of glutathione; (2) in ATP production, via hydrogen sulfide (H2S) production, serving as a donor to electron transport chain (ETC), and (3) as a carbon source for biomass and energy production. In the present review, emphasis will be given to the role of cysteine as a carbon source, focusing on the metabolic reliance on cysteine, benefiting the metabolic fitness and survival of cancer cells. Therefore, the interplay between cysteine metabolism and other metabolic pathways, as well as the regulation of cysteine metabolism related enzymes and transporters, will be also addressed. Finally, the usefulness of cysteine metabolic route as a target in cancer treatment will be highlighted. Edited by: Keywords: cysteine, cysteine metabolism, cysteine transport, cancer metabolic remodeling, targeting cysteine Fatima Baltazar, route University of Minho, Portugal Reviewed by: Andreas Papapetropoulos, INTRODUCTION National and Kapodistrian University of Athens, Greece Posited as a glutathione precursor or as a source of sulfur and carbon, cysteine contributes for Cesare Indiveri, cancer cell strongness and prosperity, allowing their survival upon stressful microenvironmental University of Calabria, Italy conditions and upon drugs exposure (1, 2).
    [Show full text]
  • GAPDH Enhances the Aggressiveness and the Vascularization of Non-Hodgkin’S B Lymphomas Via NF-Κb-Dependent Induction of HIF-1Α
    Leukemia (2015) 29, 1163–1176 © 2015 Macmillan Publishers Limited All rights reserved 0887-6924/15 www.nature.com/leu ORIGINAL ARTICLE GAPDH enhances the aggressiveness and the vascularization of non-Hodgkin’s B lymphomas via NF-κB-dependent induction of HIF-1α J Chiche1,2, S Pommier1,2,3, M Beneteau1,2, L Mondragón1,2, O Meynet1,2, B Zunino1,2, A Mouchotte1,2, E Verhoeyen1,2, M Guyot2,4, G Pagès2,4, N Mounier5, V Imbert2,6, P Colosetti2,7, D Goncalvès2,7, S Marchetti2,7, J Brière8, M Carles1,2,3, C Thieblemont8 and J-E Ricci1,2,3 Deregulated expression of glycolytic enzymes contributes not only to the increased energy demands of transformed cells but also has non-glycolytic roles in tumors. However, the contribution of these non-glycolytic functions in tumor progression remains poorly defined. Here, we show that elevated expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), but not of other glycolytic enzymes tested, increased aggressiveness and vascularization of non-Hodgkin’s lymphoma. Elevated GAPDH expression was found to promote nuclear factor-κB (NF-κB) activation via binding to tumor necrosis factor receptor-associated factor-2 (TRAF2), enhancing the transcription and the activity of hypoxia-inducing factor-1α (HIF-1α). Consistent with this, inactive mutants of GAPDH failed to bind TRAF2, enhance HIF-1 activity or promote lymphomagenesis. Furthermore, elevated expression of gapdh mRNA in biopsies from diffuse large B-cell non-Hodgkin’s lymphoma patients correlated with high levels of hif-1α, vegf-a, nfkbia mRNA and CD31 staining. Collectively, these data indicate that deregulated GAPDH expression promotes NF-κB-dependent induction of HIF-1α and has a key role in lymphoma vascularization and aggressiveness.
    [Show full text]
  • A Developmental Analysis of the Enolase Isozymes from Ricinus Communis'
    Plant Physiol. (1992) 99, 748-750 Received for publication October 28, 1991 0032-0889/92/99/0748/03/$01 .00/0 Accepted December 2, 1991 Communication A Developmental Analysis of the Enolase Isozymes from Ricinus communis' Jan A. Miernyk* and David T. Dennis Seed Biosynthesis Research Unit, U.S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, Illinois 61604 (J.A.M.); and Biology Department, Queen's University, Kingston Ontario, Canada K7L 3N6 (D. T.D.) ABSTRACT clarified by centrifugation as previously described (11). In Enolase activity was measured in clarified homogenates of Ricinus, it is difficult to determine the precise date of polli- various tissues during the life cycle of the castor oil plant (Ricinus nation. Hence, a staging system was developed, based upon communis L. cv Baker 296). The proportions of total activity due seed size, endosperm fresh and dry weights, percentage of to the plastid and cytosolic isozymes were determined after total lipid, and seed coat morphology, similar to the system separation by ion-exchange chromatography. The contribution of described for developing cotton seeds (14). For germinating the plastid isozyme varied from more than 30% of the total at the endosperm, quiescent seeds were imbibed overnight in run- midpoint of endosperm development to less than 1% in mature ning tap water (day 0), then planted in trays containing leaves and roots. During endosperm development, enolase activ- vermiculite and placed ity increased to a peak coincident with the maximum rate of in a glasshouse at 28°C and ambient storage lipid accumulation, then decreased to nearly undetecta- light.
    [Show full text]
  • Isomerases from Three Clostridial Species YUEN WAN SHING, JAMES M
    JouRNAL oF BACToOLOGY, Apr. 1975, p. 177-184 Vol. 122, No. 1 Copyright 0 1975 American Society for Microbiology Printed in U.SA. Psychrophilic, Mesophilic, and Thermophilic Triosephosphate Isomerases from Three Clostridial Species YUEN WAN SHING, JAMES M. AKAGI,* AND RICHARD H. HIMES Departments of Biochemistry and Microbiology,* University of Kansas, Lawrence, Kansas 66045 Received for publication 27 December 1974 Triosephosphate isomerase was purified to homogeneity as judged by analyti- cal gel electrophoresis from Clostridium sp. strain 69, Clostridium pasteurianum, and C. thermosaccharolyticum, which grow optimally at 18, 37, and 55 C, respectively. Comparative studies on these purified proteins showed that they had the same molecular weight (53,000) and subunit molecular weight (26,500). They were equally susceptible to the active site-directed inhibitor, glycidol phosphate. However, their temperature and pH optima, as well as their stabilities to heat, urea, and sodium dodecyl sulfate, differed. The proteins also had different mobilities in acrylamide gel electrophoresis. This difference in ionic character was also reflected in the elution behavior of the enzymes from hydroxyapatite and in the isoelectric points determined by isoelectric focusing in acrylamide gel. The amino acid composition of these proteins showed that the thermophilic enzyme contains a greater amount of proline than the other enzymes. The ratio of acidic amino acids to basic amino acids was 1.79, 1.38, and 1.66 for the thermophilic mesophilic and psychrophilic enzymes, respectively. This is consistent with the relative iscelectric point values of these three enzymes. The biochemical basis for the loss in viability Enzyme assay. Triosephosphate isomerase activ- of psychrophiles at moderate temperatures is ity was routinely measured in the direction from now generally accepted to be protein denatura- DL-glyceraldehyde-3-phosphate to dihydroxyacetone tion (4, 8, 12).
    [Show full text]
  • Neuron-Specific Enolase As an Index of Neuronal Regeneration and Reinnervation’
    0270-6474/83/0305-0915$02.00/O ‘The Journal of Neuroscience Copyright 0 Society for Neuroscience Vol. 3, No. 5, pp. 915-923 Printed in U.S.A. May 1983 NEURON-SPECIFIC ENOLASE AS AN INDEX OF NEURONAL REGENERATION AND REINNERVATION’ TAKAAKI KIRINO,*, ’ MILTON W. BRIGHTMAN,*, WOLFGANG H. OERTEL,$ 4 DONALD E. SCHMECHEL,$ 5 AND PAUL J. MARANGOSt 6 * Laboratory of Neuropathology and Neuroanatomical Sciences and $ Laboratory of Clinical Sciences, National Institutes of Health, Bethesda, Maryland 20205 Received August 3,1982; Revised October 18, 1982; Accepted November 9,1982 Abstract Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. Another enolase isoenzyme, non-neuronal enolase (NNE), occurs in glial cells. The purpose of this study was to follow any changes in NSE and/or NNE in cranial motor neurons after separation of their cell bodies from their axon terminals. One hypoglossal nerve in the rat and the cynomolgus monkey was thus crushed or cut and, after a given period, the brains were perfusion fixed. Immunocytochemistry, using anti-rat NSE and NNE or anti-human NSE and NNE, was performed on Vibratome-sectioned specimens of the hypoglossal nuclei. In the rat, NSE immunostaining decreased in the affected neurons 2 to 10 days following axonal injury. The change was greatest on the 10th day. Twenty days following nerve crush, NSE staining began to recover on the operated side and by the 45th day had returned to normal levels. NSE changes in the monkey were similar to those in the rat. In rats, where the nerve was cut and the proximal stump was translocated to a normally innervated muscle to inhibit re-formation of synaptic contacts, the NSE remained low for 60 days after nerve injury.
    [Show full text]