Efficient Professional and Antibody Platforms RCHY1 Antibody Basic information: Catalog No.: UMA60398 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: Protein A affinity purified Useful Information: WB:1:500-1:1000 ICC:1:50-1:200 Applications: IHC:1:50-1:200 FC:1:100-1:200 Reactivity: Human, Rat Specificity: This antibody recognizes RCHY1 protein. Immunogen: Recombinant protein Pirh2, also known as N-terminal-interacting protein (ARNIP), ZN363 or CHIMP, has -induced ubiquitin-protein ligase activity, promoting p53 degradation. The protein physically interacts with p53 and the resulting degradation of p53 renders Pirh2 an oncogenic protein as the loss of p53 function contributes to malignant tumor development. The Description: encoding for the protein maps to 4q21.1 and transcription of this gene is regulated by p53. Pirh2 expression decreases the level of p53 and a decrease of endogenous Pirh2 expression ups p53 levels. Pirh2 is therefore considered, together with MDM2, to be acting as a negative reg- ulator of p53 function. Uniprot: Q96PM5(Human) BiowMW: 30 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Western blot analysis of Pirh2 on different cell lysate using anti-Pirh2 antibody at 1/1,000 dilu- tion. Positive control： Line1: HelaLine2: A549 Line3: MCF-7 Line4: PC-12

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms ICC staining Pirh2 (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraform- aldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraf- fin-embedded human tonsil tissue using an- ti-Pirh2 antibody. Counter stained with hematox- ylin.

Flow cytometric analysis of PC-12 cells with Pirh2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]