Session 1 EVOLUTION AND ENDOGENOUS RETROVIRUSES MONDAY 5/19/2014, 7:30 PM S. Goff / H. Levin

Presentation of the Ute Von Schwedler Prize for Retrovirology to Suzannah J. Rinh # lname Title Talk Length 1 Rihn Uneven mutational robustness of HIV-1 integrase 10 2 Etienne Hominid restriction factors and their evolution influence host susceptibility to circulating 10 lentiviruses 3 Blanco Melo The evolutionary origins of the HIV-1 restriction factor, tetherin 10 4 Greenberg ZNF91 and ZNF93 evolved to restrict SVA and L1 mobile elements in ape 10 5 Metzger Amplification of the steamer retrotransposon in clam leukemia 10 6 Blomberg piRNA sequences are common in HERVs—An antiretroviral restriction mechanism? 10 7 Lim Discovery of novel lemur retroviruses 10 8 Diehl Rise and fall of an ancient retroviral lineage revealed in the genomes of modern mammals 10 9 Williams Mining deep sequencing data for novel HERVs 10 10 Mulder HIV-1 interaction with HERV-K (HML-2) envelopes derived from human primary lymphocytes 10

11 Robinson Reconstructing the cell-entry pathway of HERV-K 10 12 Battini Identification of a receptor for FeLV-D and its endogenous relatives 10

Session 2 ENTRY TUESDAY 5/20/2014, 9:00 AM R. Swanstrom / G. Melikian # lname Title Talk Length 13 Jolly The retromer complex interacts with HIV-1 envelope glycoprotein and regulates intracellular 10 trafficking 14 Munro Conformational dynamics of single HIV-1 Env trimers on the surface of native virions 10 15 Pham CD4 receptor-engagement induces structural rearrangement of HIV 10 16 Herold Comparative analysis of HIV-1 entry at the plasma membrane and from endosomes 10 17 Melikian Visualization of single entry sites in cells expressing alternatively spliced receptor 10 isoforms 18 Marin Distinct requirements for pore dilation upon HIV-cell and -mediated cell-cell fusion 10 19 Gardner A CCR5-mimetic sulfopeptide converts CD4-Ig to an exceptionally broad and potent inhibitor of 10 HIV-1 entry 20 Hain ITK plays a central role in integrating signaling pathways required for HIV-1 - T-cell fusion 10

Page 1 of 14 21 Symeonides Syncytia revisited—HIV-infected T lymphocytes can form small, mobile syncytia that transfer virus 10 at sites of transient cell-cell contact 22 Sewald CD169+ macrophages mediate MLV trans-infection of B-1 cells in 10 vivo 23 Menager Mechanisms of dendritic cell-mediated transfer of HIV-1 to CD4+ T lymphocytes 10 24 Joseph HIV-1 macrophage tropism is a distinct Env phenotype that includes the ability to use low levels of 10 CD4 for entry and is a relatively rare phenotype among all virus isolates Session 3 POST-ENTRY I TUESDAY 5/20/2014, 2:00 PM Z. Ambrose / S. Ross # lname Title Talk Length 25 Olson SAXS analysis reveals structural model of the RT initiation complex and conservation of tRNA 10 structural mimicry in the HIV-1 5´UTR 26 Takeuchi AMPK-RPK is an essential host factor for optimal HIV-1 capsid disassembly to promote HIV-1 10 cDNA synthesis in human cells 27 Hulme Early steps of reverse transcription facilitate HIV-1 uncoating 10 28 Peng Quantitative microscopic analysis of functional HIV post-entry complexes 10 29 Ambrose Visualizing HIV-1 uncoating and nuclear entry by confocal and super resolution 10 30 Di Nunzio New insights in the role of nuclear pore basket nucleoporins in chromatin remodelling and HIV 10 integration 31 Stavrou MLV reverse-transcribed DNA induces innate immune responses that limit virus infection in 10 vivo 32 Cimarelli The susceptibility to Vpx and nucleosides during the early phases of myeloid cells infection are 10 determined by distinct viral determinants in the HIV-1 and HIV-2/SIVSM lineages

33 Rasaiyaah Cloaking of lentiviral replication by CPSF6 and cyclophilins in human monocyte derived 10 macrophages 34 Case The HIV-1 core shields viral DNA from the cGAS cytosolic sensing pathway 10 35 Maelfait Transfer of antiviral immunity by incorporation of the secondary messenger cGAMP into HIV-1 10 virions. 36 Towers TRIM5α constructs mixed-linkage ubiquitination to recruit the proteasome and simultaneously 10 trigger innate signaling KEYNOTE SPEAKER I TUESDAY 5/20/2014, 7:30 PM Mary Carrington, NCI Session 4 POSTER SESSION I TUESDAY 5/20/2014, 8:30 PM

# lname Title Talk Length 37 Acosta-Hoyos HIV nucleocapsid inhibits ATP-mediated excision by HIV reverse transcriptase 38 Aguiar Reactivation of latent HIV-1 by new semi-synthetic ingenol esters 39 ALI A novel TRIM protein restricts HIV-1 integration by targeting integrase for proteasomal degradation during infection Page 2 of 14 40 ALI Non-canonical HIV-1 Tat secretion through the cellular Na+ K+ ATPase enhances HIV-1 infectivity 41 Anderson Development of droplet digital PCR approaches for detection of HIV with single copy sensitivity

42 Apolonia The hitchhikers’ guide to the viral particle 43 Ariumi Tumor suppressor restricts LINE-1 retrotransposition 44 Arriagada The cytoplasmic dynein complex is important for MLV infection 45 Bacharach MLV infection of mitotic cells—Reduced restriction and microtubule-independent chromosome- targeting 46 Ball Structural and biophysical studies of a second self-associating domain of a Spumavirus Gag

47 Bamunusinghe Most infectious xenotropic and polytropic gammaretroviruses are derived from recombination of two or more endogenous retroviruses 48 Behrens Multiplication of Rev-associated NES domains rescues HIV-1 virion production in murine cells

49 Benichou Structural basis for the inhibition of HIV-1 Nef by a high-affinity binding single-domain antibody fragment 50 Bhutoria Molecular modeling of IN-binding domain of INI1 reveals structural similarity to PLAA and residues involved in IN-INI1 interaction

51 Bitzegeio Interferon-induced, envelope dependent restriction of SIVMAC in human lymphocytes

52 Blomberg Classification and characterization of HERVs 53 Blondot Proteomic strategy to highlight how HIV-1 Vpr affects the cellular proteome 54 Bouaziz Structural studies of new bevirimat derivatives with the Gag CA-SP1 junction 55 Browne Interleukin-1 beta promotes retroviral infection and pathogenesis 56 Bruce ZBTB2 regulates HIV-1 transcription 57 Chen The Env protein of SIVsm contributes to the control of BST-2/tetherin 58 Comas-Garcia Single-molecule studies on HIV-1 Gag/RNA interactions 59 Courgnaud Interaction of HIV-2 capsids from primary isolates with cellular factors and disease progression

60 Cyburt Evidence for additional cellular cofactors required for Vpu activity 61 Czubala TGF-β induces a potent post-entry restriction to HIV-1 infection independent of SAMHD1 in immature langerhans cells 62 Daly Effect of non-canonical ribonucleotide incorporation by HIV-1 reverse transcriptase on HIV-1 genomic mutagenesis 63 Datta Associative interactions of the SP1 domain of HIV-1 Gag 64 De Houwer Reduced nuclear import of HIV carrying the transportin-SR2 interaction mutant INR263A/K264A

Page 3 of 14 65 De Rijck BET-independent MLV-based vectors integrate efficiently and associate less with proto-oncogene transcription start sites 66 Dharan Efficient HIV-1 uncoating requires microtubules and microtubule associated proteins (MAPs)

67 Diaz The E3 ubiquitin ligase protein Cullin3 regulates HIV-1 transcription in an NF-κB and IRF- dependent manner 68 Diaz-Griffero Contribution of GTPase activity to the ability of MxB to block HIV-1 infection 69 Diaz-Griffero Effects of human SAMHD1 polymorphisms on HIV-1 susceptibility 70 Domaoal Pre-steady state kinetic analysis of HIV-1 reverse transcriptase for non-canonical ribonucleoside triphosphate incorporation and DNA synthesis from mutagenic ribonucleotide-containing DNA template 71 Du Evolutionarily conserved pressure for the existence of multiple distinct substrate recognition domains in HIV-1 Vif 72 Duchon Mechanism of human Lysyl-tRNA synthetase/tRNALys primer recruitment and packaging into HIV-1 73 Dudley To the ER and back again—An unusual trafficking pattern for the Rem protein 74 Durham Distinct functional determinants in the HIV-1 gp41 cytoplasmic tail regulate cell-free versus cell- to-cell infection 75 Ebrahimi Mohammadi Signatures of APOBEC3 on the of endogenous and exogenous retroviruses 76 Ebrahimi Mohammadi Source of CpG depletion in HIV 77 Eckwahl Elucidating the retroviral RNA packageome 78 Endsley The intracellular domain of ADAM10 binds HIV-1 integrase to facilitate nuclear trafficking of the pre-integration complex 79 Erikson Mouse Siglec1 enhances the trans-infection of MLV in a sialic acid N-acyl side chain-dependent manner 80 Farley Development of a replication-competent lentivirus (RCL) assay for testing HIV-1-based vectors pseudotyped with a modified alphavirus envelope 81 Fassati Th17 cells master transcription factor RORγ/γt regulates HIV-1 gene expression and integration site selection 82 ferrer Imaging HIV-1 genomic RNA dimers in the cell 83 Finzi Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity 84 Fricke BI-2 destabilizes HIV-1 cores during infection and prevents binding of CPSF6 to the HIV-1 capsid

85 Fujita SAMHD1-independent functions of HIV-2 Vpx protein 86 Gao HIV-1 hijacks RuvB-like 2 to balance viral protein expression 87 Grewe Substitution of the Rev/RRE system by inclusion of codon-optimized sequences in an HIV-derived lentiviral vector 88 Grznárová Intracellular transport of the MPMV envelope glycoprotein

Page 4 of 14 89 Gummuluru GM3 functionalized plasmonic nanoparticles recapitulate CD169-dependent HIV-1 uptake and sequestration within mature dendritic cells 90 Guo A family logo of HIV-2/SIVmac Vpx and its roles in Vpx functions 91 Gupta Linking inhibition of late replication steps by an allosteric inhibitor HIV integrase with multimerization of specific protein domains

Session 5 APOBEC, VIF, MUTAGENESIS WEDNESDAY 5/21/2014, 9:00 AM V. Simon / L. Mansky # lname Title Talk Length 92 Du Blocking HIV-1 Vif function and viral replication in CD4+ T cells by CBFβ variants without 10 compromising its cellular function 93 Letko Exploiting viral-host variation to probe for the HIV-1 Vif-A3G interface 10 94 Pollpeter APOBEC3 interference with HIV-1 reverse transcription 10 95 Xiong Structural insight into the DNA substrate selection mechanism of APOBEC3G 10 96 Bohn APOBEC3A crystal structure reveals a role of functional dimerization in substrate recognition 10

97 Harris A structural model of the APOBEC3F-Vif interaction informed by adaptation and mutagenesis 10 studies 98 Pathak APOBEC3F and APOBEC3G can co-mutate the same HIV genome 10 99 Sato APOBEC3F potently promotes HIV-1 diversification and evolution in humanized mouse model 10

100 Simon HIV adaptation and evolution in humanized mice 10 101 Ikeda Natural variation in HIV-1 Vif determines the ability of endogenous levels of the polymorphic 10 APOBEC3H protein to suppresses virus replication in primary T cells 102 Ooms HIV-1 Vif adaptation to APOBEC3H in chronically HIV infected patients 10 103 Mansky HIV-1 and HIV-2 lethal mutagenesis mediated by striking levels of G-to-A hypermutation in a 10 single round of replication 104 Dudley Function of the MMTV Rem C-terminal product in innate immunity 10

Session 6 POSTER SESSION II WEDNESDAY 5/21/2014, 2:00 PM

# lname Title Talk Length 105 Haim Ligand-independent activation of the HIV-1 Envs—Exposure to cold induces transition to an unstable CD4-bound state 106 Halambage Replication of an HIV-1 capsid mutant is enhanced by the small molecule inhibitor PF-3450074

107 Halm Characterization and expression of ERV-Fc Env proteins encoded in primate genomes 108 Hammonds Siglec-1 facilitates attachment and internalization of exogenous HIV-1 and contributes to Virus containing compartment formation in infected monocyte-derived macrophages Page 5 of 14 109 Herschhorn A small-molecule inhibitor that interacts with the unliganded conformation of HIV-1 envelope glycoproteins 110 Hirnet HIV-1 variants resistant to PPARγ signaling contain mutations in nef and gp41 111 Hizi The dUTPase-related gene of BIV is critical for viral replication, despite the lack of enzymatic activity of the encoded protein 112 Hogg Mechanism of nonsense-mediated decay inhibition by the RSV RNA stability element 113 Hollenbaugh dNTP pool modulation dynamics by SAMHD1 protein in monocyte-derived macrophages 114 Hunegnaw Interaction between HIV-1 Nef and Calnexin regulates cholesterol transporter ABCA1 115 Hwang Defective gene patterns of PERV from SNU miniature pigs 116 Imle Functional relevance of the association of HIV-1 Nef with the exocyst complex and β-catenin

117 Ita Population level sequencing reveals a reproducible pattern of Env sequence evolution and antibody-escape in SIV-infected rhesus macaques 118 Ivanov Characterizing SAMHD1 tetramerization and dNTPase activity by analytical ultracentrifugation and NMR 119 Iwatani Lack of association between intact/deletion polymorphisms of the APOBEC3B gene and HIV-1 risk

120 Jaguva Vasudevan Target cell APOBEC3A inhibits the non integrating HIV-1 and MLV 121 Jain HIV accessory protein function—Exploring VPU dependent host factor degradation 122 Janvier BST2, a restriction factor of HIV-1 release—Role of the ubiquitination pathway in its regulation and countermeasure by the viral protein Vpu 123 Jauregui Vpx-mediated degradation of SAMHD1 is not DDB1-dependent 124 Joshi Hyperthermia accelerates HIV production through Hsp90-mediated promotion of viral transcription

125 Kane Cross-species analysis reveals differential composition of a lentivirus Vif-hijacked ubiquitin- ligase complex 126 Kane Antiviral activity and subcellular localization of Mx2 127 Katuwal Determinants of restriction of HIV-1 by Colobus guereza APOBEC3A 128 Keppler Impact of raltegravir on HIV-1 RNA and DNA species following initiation of antiretroviral therapy in treatment-naïve patients 129 Kirchhoff Identification of IFI16 as HIV restriction factor by combining evolutionary signatures and functional analyses 130 Kono Designing soluble and functional A3G NTD variants 131 Kozak Limited host escape mutations of the XPR1 gammaretrovirus receptor 132 Krivdova Inhibition of HIV-encoded Vif by PAP and its impact on cellular immune defense 133 Kroupa M-PMV matrix protein mutants and their interaction with membrane phospholipids 134 Ku Identification of pauses during formation of HIV-1 virus like particles

Page 6 of 14 135 Kubo Identification of a novel interferon-stimulated gene whose product significantly restricts retrovirus replication 136 Kutluay Global identification of the RNA targets of HIV-1 Gag during virion genesis 137 Landi Genome-wide shRNA screening identifies new host factors involved in HIV-1 Vpu- and Nef- mediated CD4 downmodulation 138 La Porte An HIV-1 IN mutant that is proficient in binding to LEDGF but is defective for binding to INI1/SMARCB1 and SAP18 is impaired for integration in vivo 139 Latham Developing novel allosteric fragment inhibitors of HIV-1 reverse transcriptase for HIV prevention

140 Levin A long terminal repeat retrotransposon in Schizosacchromyces japonicas integrates into upstream sequences of tRNA genes in S. pombe 141 Li Assembly pathway of retroviral intasomes 142 Li Studying the concentration dependency of Fv1b restriction using a Doxycycline- inducible expression system 143 Li Molecular evolution of HIV-1 in an individual who developed glycan-specific broadly cross-reactive neutralizing antibodies 144 Lin A transdominant Tat mutant disrupts HIV-1 Rev function by sequestering the DEAD box protein DDX1 145 Lindemann A concerted function of arginine residues in the prototype FV Gag C-terminus mediates viral and cellular RNA encapsidation 146 Lukic Modulation of HIV-1 uncoating by host cell proteins 147 Maldonado Characterization of HTLV-1 particle morphology and Gag stoichiometry 148 Mamede HIV-1 uncoating and reverse transcription—Another connection between capsid and reverse transcription 149 Manganaro Tumor suppressor cylindromatosis (CYLD) controls HIV transcription in a NF-κB/NFAT dependent manner 150 Marlett Global analysis of cellular phosphorylation events associated with HIV-1 infection—Importance of Aurora Kinase A (AURKA) 151 Matsuda Co-expression of foreign proteins tethered to HIV-1 envelope protein by introducing an intervening second membrane-spanning domain 152 Matsui CBFβ stabilizes HIV-1 Vif by inhibititing MDM2-mediated degradation 153 Maunder Infection studies of accessory gene-deficient variants of HIV-1 in C8166-45 cells towards development of a replication competent lentivirus (RCL) assay for clinical vector release 154 McKee A conserved RXL binding motif in SAMHD1 is required for its interactions with the cell cycle regulatory proteins cyclin A2, CDK1 and CDK2 155 Mendonca HIV-1 Nef impacts the protein content of mature viral particles 156 Miles MxB restriction of HIV-1 infection 157 Morrison Primate lentivirus infection is blocked in Megachiropteran cells by a cyclophilin-dependent mechanism that acts at an early post-entry step

Page 7 of 14 158 Mousseau The Tat inhibitor didehydro-Cortistatin A establishes a state of deep-latency that prevents HIV-1 reactivation 159 Mueller HIV-1 Nef and Vpu are functionally redundant broad-spectrum modulators of cell surface receptors including tetraspanins 160 Mukherjee Alpha-helices in the coiled coil – linker region govern rhesus TRIM5α assembly and restriction

161 Muriaux Requirement of the Rac1-IRSp53-Wave2 signaling pathway during HIV-1 Gag assembly and particle release in CD4 T cells 162 Naghavi FEZ1 regulates Kinesin-1 motor activity to promote movement of HIV-1 towards the nucleus

163 Nakashima Fine-tuned HIV-1 Vif-interaction interface of anti-retroviral cytidine deaminase APOBEC3F 164 Nakayama Novel mutant HIV-1 strains with high degree of resistance to cynomolgus macaque TRIMCyp generated by random mutagenesis 165 Neil Counteraction of tetherin by Env proteins in natural HIV-2 infection 166 Neil Direct binding of a phosphorylation mutant of Vpu to Clathrin rescues tetherin antagonism in the absence of ubiquitin ligase recruitment 167 Nepveu-Traversy TRIM5α motifs relevant to the SUMOylation pathway are determinants for the activation of innate immunity pathways 168 Nikolaitchik Deciphering the role of the Gag-Pol ribosomal frameshift signal in HIV-1 RNA genome packaging

Session 7 POST-ENTRY II WEDNESDAY 5/21/2014, 7:30 PM E. Campbell / S. Wilson # lname Title Talk Length 169 Lee CPSF6 Interaction with HIV-1 specifies a reproducible global pattern of viral integration 10 170 Burse Cyclophilin A enhances TRIM5α-dependent restriction of HIV-1 by accentuating the block to 10 reverse transcription 171 Campbell Preventing rhesus TRIM5α degradation via chaperone mediated autophagy increases restriction 10 of HIV-1 172 Ivanov Activation of the Ubc13-Ub adduct by the RING domain of TRIM5α 10 173 McCarthy Ancient primate lentiviruses selected for evolutionary innovation within the first variable loop of old 10 world monkey TRIM5α 174 Pornillos TRIM5α dimerization implies direct structural coupling of the capsid-binding SPRY domain to the 10 tripartite motif 175 Goldstone Structure of the core self-association domain of Trim5α 10 Awarding of 8th Annual Andy Kaplan Prize to Caroline Goujoun 176 Goujon Converting human MX1 GTPase into a potent HIV-1 inhibitor 10 177 Fricke MxB binds to the HIV-1 core and prevents the uncoating process of HIV-1 10 178 Busnadiego Host and viral determinants of Mx2 antiretroviral activity 10 179 Wei Viral evasion of the anti-HIV-1 activity of MX2 10 Page 8 of 14 180 Liang HIV-1 evades MxB restriction 10 181 Fribourgh Structural insight into HIV restriction by myxovirus resistance protein 2 (MxB) 10

Session 8 VPX, SAMHD1, AND VPR THURSDAY 5/22/2014, 9:00 AM R. Gummuluru / F. Diaz-Griffero # lname Title Talk Length 182 Taylor Structural basis of lentiviral hijacking of the host ubiquitin ligase machinery 10 183 Tang SAMHD1 in viral restriction and cellular dNTP regulation 10 184 Wu Identification of cellular proteins interacting with the retroviral restriction factor SAMHD1 10 185 Diaz-Griffero SAMHD1 Triphosphohydrolase activity is necessary but not sufficient for HIV-1 restriction 10 186 Gramberg The antiviral activity of SAMHD1 in the mouse affects reverse transcription and correlates with its 10 phosphorylation status. 187 Shingai Vpx mediates suppression of SAMHD1 during pathogenic SIVmac infections of rhesus macaque 10

188 Lenzi Mechanistic variations among reverse transcriptases of HIV and SIV variants 10 189 Bloch Vpx promotes HIV-1 DNA synthesis in resting CD4+ T cells but infection is blocked at integration 10

190 Berger Correlation between primate lentiviral Vpr interaction with the SLX4 complex and induction of 10 G2/M arrest in human cells 191 Fregoso Activation of the DNA damage response is a conserved function of primate lentiviral Vpr proteins 10

192 HERATE Uracil DNA glycosylase is required for optimal virus spreading in the primary target cells of HIV-1 10

193 Miller HIV-1 Vpr is necessary for gp120 production and incorporation into myeloid cell-derived progeny 10 virions

Session 9 INTEGRATION AND INTEGRASE THURSDAY 5/22/2014, 2:00 PM M. Kvaratskhelia / A. Engelman # lname Title Talk Length 194 Serrao Mechanistic basis of palindrome site recognition at sites of retroviral integration 10 195 Grandgenett RSV intasome capable of concerted integration is kinetically trapped by HIV-1 strand transfer 10 inhibitors 196 Larue BET proteins act as a bimodal tether of MLV integrase and mononucleosomes 10 197 Aiyer Altering MLV integration through disruption of the integrase and BET protein family interaction 10

198 Bushman Gene therapy for SCID-X1—Comparing trials carried out using gammaretroviral vectors with 10 intact or deleted (SIN) LTRs by tracking integration site distributions 199 Demeeulemeester HIV-1 integrase variants retarget viral integration on both a local as well as on a global scale 10

Page 9 of 14 200 Lusic Nuclear architecture favors HIV-1 integration into a subset of active genes bound to the nuclear 10 pore in CD4+ T-cells 201 Singh Analysis of 3-million independent HIV-1 integration sites identifies a link with mRNA splicing 10

202 Hughes The role of HIV integration sites in extensive clonal expansion of infected cells in patients 10 203 Fadel TALEN knock-out of human PSIP1 (LEDGF/p75 gene)—Characterization of HIV-1 10 phenotypes and ALLINI mechanism 204 Kvaratskhelia A new class of multimerization selective inhibitors of HIV-1 integrase 10 205 Jurado Inhibition of HIV-1 particle maturation by allosteric integrase inhibitors 10

KEYNOTE SPEAKER II THURSDAY 5/22/2014, 8:30 PM

Paul Bieniasz, The

Session 10 POSTER SESSION III THURSDAY 5/22/2014, 8:30 PM

# lname Title Talk Length

206 Olety Amaranath PI(4,5)P2 binding—Differences between HIV-Gag and the PH domain of PLCδ1

207 Ostrowski Rab27a controls HIV-1 Gag trafficking and virus assembly by regulating plasma membrane levels of PI(4,5)P2 208 Pache Identification of host cell mechanisms restricting HIV-1 replication 209 Pacheco Adaptation of HIV-1 to common marmoset BST-2 and APOBEC3G 210 Paillart Relocation of APOBEC3G mRNA by the HIV-1 Vif protein 211 Patel Reconstructing the life history of ERV-Fc in Old World primates 212 Pawlica Dynein motor complexes and microtubules are important for HIV-1 uncoating 213 Pocock Dissecting HIV-1’s post-transcriptional stages using an imaging based approach 214 Potempa The catalytic activity of the HIV-1 Protease is dramatically enhanced by RNA 215 Qi A YW motif in helix 2 of the gp41 cytoplasmic tail mediates cell type- and FIP1C-dependent incorporation of the HIV-1 envelope glycoprotein complex into virions 216 Quenneville A VLP-Vpx treatment of quiescent primary CD4+ T cells allows the development of a HIV-1 latency model 217 Rahmberg High-throughput transcriptional analysis of HIV/SIV restriction and dependency factors in CD4+ T cells 218 Rice Deciphering the role of RSV Gag within the nucleus 219 Rizvi Role of purine rich sequences in MPMV genomic RNA (gRNA) packaging 220 Robinson Naturally-arising polymorphisms in HIV-2 Gag increase capsid assembly kinetics and VLP production 221 Roesch Vpr potentiates innate sensing of HIV-1 infected cells 222 Rosa Understanding the nature of the Nef requirement for HIV-1 infectivity Page 10 of 14 223 Rouzina Studies of the HIV-1 Reverse Transcriptase DNA polymerase and pyrophosphatase activities by applying stretching force to the template DNA strand 224 Ruiz HIV-1 subtype-specific polymorphism in Tat basic domain - Effects on cellular uptake and transcellular transactivation 225 Rye-McCurdy Distinct binding interactions of RSV NC and MA to Psi and non-Psi RNA 226 Sabo Aladin, a nuclear pore complex-associated protein, affects the late stage of the retrovirus life cycle

227 Sakuragi SL1 revisited—Functional analysis of the structure and conformation of HIV-1 psi RNA. 228 Salamango Elucidating the mechanism of MLV envelope glycoprotein recruitment to viral assembly sites

229 Salamango Evidence for additional cellular cofactors required for Vpu activity 230 Sanborn Functional correlates of mother-to-infant HIV transmission 231 Sardo The dynamics of HIV-1 RNA on the plasma membrane 232 Schmidt Antagonism of SAMHD1 restriction of HIV in resting CD4 T cells and depletion of SAMHD1/dNTP elevation are separable activities of Vpx 233 Setiawan The effects of viral escape from HLA B57-restricted cytotoxic T lymphocytes on viral replication and innate responses in primary cells 234 Setz Mutation of the highly conserved Ser-40 of HIV-1 p6 to Phe does not affect structure but enhances membrane association, polyubiquitination and immunogenicity of Gag 235 Sewald MLV Gag localizes to the uropod in migrating lymphocytes 236 Sherpa The HIV-1 Rev response element exists in two alternative secondary structures which promote different replication activities 237 Sherrill-Mix HIV and host cell RNA abundance analyzed by RNA-Seq 238 Silvas The Vif/CBFβ interface provides a structural basis for molecular evolution of species specificity of retroviral replication 239 Slaughter Two distinct mechanisms of resistance to allosteric HIV-1 integrase inhibitors 240 Sloan Mutations in capsid overcome IFITM-mediated restriction of HIV-1 241 Smith Developing a series of 1- hydroxy-1,8-naphthyridin-2(1H)-one compounds to overcome HIV-1 drug resistance 242 Soll Undiscovered cis-acting RNA elements in the HIV-1 genome 243 Song Elements of Lentiviral P6 domain modulating viral assembly 244 Stieh Hotspots of SIV infection in the female reproductive tract after vaginal exposure of rhesus macaques to single round dual reporter vector 245 Sukegawa Suppressor of cytokine signaling 1 counteracts rhesus macaque TRIM5α-mediated inhibition of HIV-1 production 246 Swann Sulfonation-dependent control of HIV-1 replication—SULT1A1 regulates viral reverse transcription

247 Swanson Regulation of HIV-1 Gag expression by SR proteins

Page 11 of 14 248 Tanaka Major homology region mutations arrest HIV-1 Gag as a membrane-targeted assembly intermediate containing the cellular factors ABCE1 and DDX6 249 Tedbury Cell type-dependent requirement for the gp41 cytoplasmic tail is regulated by the trafficking factor Rab11a-family interacting protein 1c 250 Trinite Common gamma chain cytokines protect CD4 T Cells from death induced by HIV-1 reverse transcription independently of RT efficiency 251 Turner Selected A3G ubiquitin modification is critical for Vif induced degradation 252 Tyagi DNA-PK inhibition potently represses HIV transcription and replication by suppressing RNAP II CTD phosphorylation 253 Vermeire HIV triggers a post-integration type 1 IFN response in primary CD4+ T cells 254 Walker Increased levels of MIPs result in resistance to M-tropic HIV-1 in two elite controllers 255 Wang ZFP809, the DNA-binding component of embryonic-specific transcriptional silencing complex, is regulated by ubiquitin-dependent protein degradation 256 Wang Ventricular septal defect underlies embryonic lethality of PSIP1 / HDGFRP2 knockout mice

257 Wang Retrovirus detection by rolling circle amplification of an Integrase product 258 Wang MCPIP1 restricts HIV infection and is rapidly degraded in activated CD4+ T cells 259 Wang Expression of APOBEC3 proteins in the rhesus macaque brain 260 Wei Substrate-controlled GTP-activated SAMHD1 tetramer dNTPase 261 Wille The cis-acting genomic RNA packaging enhancer (GRPE) enhances HIV-based lentiviral vector genome packaging and infectivity 262 Wu Development of MLV based viral like particles for the delivery of toxic proteins and nuclear transcription factors 263 Wu Probing the structural determinants of human restriction factor APOBEC3H antiviral and cytidine deaminase activities 264 Wudzinska Intracellular localization of Mo-MLV integrase and a host cofactor 265 Yang Cell-to-cell transmission of HIV-1 is required to trigger innate recognition and pyroptosis of lymphoid CD4 T Cells 266 Yoshii-Kamiyama Elucidation of the mechanism of IRF-3 downregulation by HIV-1 267 Yoshinaga A screening for DNA repair enzymes related to HIV-1 life cycle 268 Zhao Determinants of HIV-1 Vif-induced G2/M cell cycle arrest 269 Zhao SAMHD1 suppresses HIV-1 through a mechanism independent of its dNTPase activity yet still distinct from LINE-1 inhibition 270 Zheng TSPO inhibits HIV-1 Env biogenesis likely by interfering with its folding

Page 12 of 14 Session 11 ASSEMBLY FRIDAY 5/23/2014, 9:00 AM K. Bishop / J. Lingappa # lname Title Talk Length

271 Luban Polysome loading of gammaretrovirus gag mRNA requires cis-acting pol sequences that recruit 10 NXF1/NXT1 and SRp20 272 Ahi Investigating function of glycogag in MLV life cycle 10 273 Kaddis Dissecting the mechanism of RSV genomic RNA encapsidation 10 274 Bernacchi Early steps in HIV-1 assembly process—How the Pr55Gag precursor selects specifically the 10 genomic RNA 275 Chen Cytoplasmic HIV-1 RNA transport is mainly driven by diffusion 10

276 Inlora The roles of RNA and PI(4,5)P2 in regulating retroviral MA membrane binding 10 277 Bouamr Host factor-mediated mechanisms of cholesterol recognition at HIV-1 budding sites 10 278 Ehrlich PTAP L domain-dependent modulation of Ca2+ signaling facilitates HIV-1 Gag 10 assembly and budding 279 Lingappa Assembly of Ty3 yeast retrotransposon capsids in human cells reveals that Ty3, like HIV-1, forms 10 ABCE1- and DDX6-containing assembly intermediates 280 Tedbury HIV-1 envelope glycoprotein incorporation requires matrix trimerization 10 281 Sarafianos Crystal structure of the native hexameric full-length HIV-1 capsid 10 282 Wight The N-terminal domain of p12 is required for core formation during maturation 10

Session 12 LATE REPLICATION, VPU, BST2/TETHERIN FRIDAY 5/23/2014, 2:00 PM S. Neil / E. Cohen # lname Title Talk Length 283 Saffarian ALIX is recruited temporarily into HIV-1 budding sites at the end of Gag assembly 10 284 Urano Recent progress in the development of HIV-1 maturation inhibitors—Structure-function analysis of 10 the maturation inhibitor binding site 285 Li Tetherin/BST-2 promotes the innate and adaptive cell-mediated immune response to retrovirus 10 infection in vivo 286 Bego HIV-1 Vpu exploits the crosstalk between BST2 and ILT7 to dampen the antiviral response of 10 plasmacytoid dendritic cells 287 Galao Retroviral retention by tetherin induces Syk recruitment to initiate proinflammatory NFkB activation 10

288 Liberatore Tetherin expression predominantly restricts cell-free virus release in vitro, but broadly 10 limits MLV dissemination in vivo 289 Kirchhoff The resistance of human tetherin to counteraction by primate lentiviral Nef proteins is not absolute 10

290 Lewinski A C-terminal residue of HIV-1 clade B Vpu supports the displacement of BST2 from Gag at the 10 cell surface and optimal virion release 291 Lukhele HIV-1 Vpu dileucine motifs are important for Vpu-mediated tetherin antagonism and virus release 10

292 Waheed Role of the Vpu-interacting protein SGTA in regulation of tetherin and HIV-1 release 10 Page 13 of 14 293 Madjo Emerging role of the autophagy-related protein Atg5 in HIV production 10 294 Liu The TIM family proteins inhibit HIV-1 release 10 Session 13 INNATE / INTRINSIC IMMUNITY SATURDAY 5/24/2014, 9:00 AM D. Evans / M. Li # lname Title Talk Length 295 Evans Modulation of natural killer cell activation by SIV 10 296 Sauter Primate lentiviral Nef and Vpu proteins tightly regulate NF-κB-dependent expression of viral and 10 antiviral genes 297 Li HIV-1 exploits antiviral TLR3 signaling to enhance infection of macrophages via chromatin 10 remodeling 298 Akiyama HIV-1 evades IFNα-induced antiviral state in target cells by exploiting CD169 in cis and in trans 10

299 Iyer Type I IFN resistance of mucosally transmitted HIV-1 10 300 Yoh PQBP1 is an innate immune receptor for HIV-1 10 301 Muñoz-Arias Blood CD4 T cells are resistant to pyroptosis during HIV-1 infection 10 302 Johnson Global landscape of HIV-mediated ubiquitination 10 303 Stephens Cellular restriction of HIV-1 by truncated Old World primate APOBEC3A proteins lacking a 10 complete deaminase domain 304 Compton Interferon induced transmembrane (IFITM) proteins incorporate into HIV-1 virions and impair viral 10 cell-to-cell spread 305 Tartour IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity 10

306 McKnight The potency of RNA-associated early-stage anti-viral factor (REAF) depends on the entry pathway 10 used by HIV to infect cells

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