Satellite Workshops Summary
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2 SATELLITE WORKSHOPS SUMMARY Satellite Workshop Asperfest 17 4 Satellite Workshop Fusarium 70 Satellite Workshop Trichoderma, Clonostachys and other biocontrol fungi 106 Satellite Workshop Colletotrichum 2020 126 Satellite Workshop Neurospora 140 Satellite Workshop Magnafest 2020 154 SUMMARY 3 SATELLITE WORKSHOP Asperfest 17 SUNDAY, FEBRUARY 16 Location: Sapienza University of Rome Building: CU022 | Side: Botanica | Floor: Ground | Room: Giacomini Registration and poster hang up, Poster and Welcome Reception (sponsored by Novozymes Inc.) 17:00 - 18:30 Odd-numbered posters 18:30 - 20:00 Even-numbered posters Judging for Novozymes Student Poster Prize, Coordinated by Ling Lu | Nanjing Normal University, Poster Session Chair Poster take down MONDAY, FEBRUARY 17 Location: Sapienza University of Rome Building: CU022 | Side: Genetica | Floor: Ground | Room: Montalenti Session I Chair: Richard Todd, Kansas State University – Norio Takeshita, University of Tsukuba 09:00 - 09:15 Welcome, introductions and announcements 09:15 - 10:45 1. Oier Etxebeste | University of the Basque Country Transcriptional networks controlling asexual development in Aspergillus nidulans: An evolutionary perspective 2. Hubertus Haas - Innsbruck Medical University Iron sensing in Aspergillus fumigatus 3. Neta Shlezinger | The Hebrew University of Jerusalem Fungal-host interactions: A duel to the (cell) death 4. Levente Karaffa | University of Debrecen Aspergillus terreus itaconic acid fermentation: the physiology behind key technological parameters 10:45 - 11:15 Coffee Break ASPERFEST 17 5 Session II Chair: Richard Todd, Kansas State University 11:15 - 11:30 N. Louise Glass | UC Berkeley Developing a high-throughput functional genomics platform for Aspergillus flavus 11:30 - 11:45 Fabio Gsaller | Innsbruck Medical University Genetic engineering of fungi exploiting pyrimidine salvage pathway- based self-encoded selectable markers Flash Talks Chair: Nancy Keller, University of Wisconsin – Michelle Momany, University of Georgia 11:45 - 12:15 1. Ignacio Bravo-Plaza | Centro de Investigaciones Biológicas Identification of the guanine nucleotide exchange factor for SAR1 in the Speed pitches filamentous fungal model Aspergillus nidulans (3 min) to posters 2. Eszter Bokor | University of Szeged The nicotinic acid pathway of Aspergillus nidulans includes a reversible conversion to 6-hydroxynicotinic acid 3. Shoki Fujita | Tohoku University CreD ubiquitination required for endocytic degradation of the maltose transporter MalP in Aspergillus oryzae 4. Sandra Garrigues | Westerdijk Fungal Biodiversity Institute Combinatorial control of transcription factors involved in sugar beet pulp utilization in the industrially relevant fungus Aspergillus niger 5. Mamun Md. Abdulla Al | The University of Tokyo Identification of novel proteins for fungal cell-to-cell communication by localization screening from multicellularity-specific uncharacterized genes 6. Hajer Alshraim Alshammri | The University of Manchester A rapid CRISPR-mediated Tet-Off system reveals the phosphoinositide kinases Stt4 and Mss4 are essential for viability of Aspergillus fumigatus 7. Amelia Barber | University of Wuerzburg Comparative genomics of Aspergillus fumigatus and the influence of agriculture on ecology and azole resistance 8. Matthew Blango | Leibniz Institute for Natural Product Research and Infection Biology Aspergillus fumigatus elicits host-derived extracellular vesicles upon infection 6 SATELLITE WORKSHOPS 9. Peter Punt | Dutch DNA Biotech & Leiden University Rewiring metabolic pathways for organic acid production in the filamentous fungus Aspergillus niger 12:15 - 12:45 David Roos | FungiDB update Community directions discussion; Elections 12:45 - 14:00 Lunch Session III Chair: David Canovas, University of Seville – Nir Osherov, Tel Aviv University 14:00 -14:15 Sjoerd Seekles | Leiden University The effect of cultivation temperature on the heat resistance of Aspergillus niger conidia 14:15 - 14:30 Irene Picazo | Centro de Investigaciones Biológicas Effects of ambient alkaline pH on gene expression: a key regulatory role for the cation-homeostasis transcription factor SltA 14:30 - 14: 45 Danielle Weaver | University of Manchester Uncovering long non-coding RNA associated with drug response in Aspergillus fumigatus 14:45 - 15:00 Gayan Abeysinghe | University of Tsukuba Exploring the variety of interactions between Fungi and Bacteria 15:00 - 15:15 Sayoko Oiki | University of Tsukuba Cellular response to farnesol and the role of nitric oxide production in Aspergillus fumigatus 15:15 - 15:30 Paul Schäpe | TU-Berlin Harnessing transcriptomic data to predict the function of proteins in the microbial cell factory Aspergillus niger 15:30 - 15:45 Anezia Kourkoulou | National and Kapodistrian University of Athens UapA-membrane lipid interactions are crucial for ER-exit, dimerization, function and expression of mammalian transporters in A. nidulans 15:45 - 16:15 Coffee break ASPERFEST 17 7 Pontecorvo Lecture (sponsored by Zymergen) Chair: Norio Takeshita, University of Tsukuba – Benjamin Knox, Zymergen, Inc. 16:15 - 17:00 Katsuya Gomi | Tohuku University How the koji mold Aspergillus oryzae produces amylolytic enzymes? 17:00 - 17:30 Election results; Novozymes student poster prizes; other discussion items 17:30 Dismiss Presenter indicated in bold type* denotes a student poster presenter 8 SATELLITE WORKSHOPS *1. Identification of novel proteins for fungal cell-to-cell communication by localization screening from multicellularity-specific uncharacterized genes Mamun Md. Abdulla Al1, Katayama Takuya1, Cao Wei2, Nakamura Shugo2, Maruyama Junichi1 1 Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan 2 INIAD, Toyo University, Bunkyo-ku, Tokyo, Japan Multicellular organisms have the feature of intercellular molecular exchange for cooperation among the cells or tissues. Multicellular fungi possess a primitive morphological structure for cell-to-cell communication, the septal pore. A specifically evolved fungal organelle Woronin body plugs the septal pore upon wounding, thereby protecting the flanking cell. However, comparative genomic approach between multicellular and unicellular fungal species has not yet been employed to investigate additional components/mechanisms in the regulation of cell-to-cell communication. As Ascomycota, one of fungal divisions, possesses a number of genetically characterized species, we performed a genomic comparison between multicellular and unicellular ascomycetes and subsequent localization screening to find novel proteins regulating the cell-to-cell communication via septal pore. In this study, we used Aspergillus oryzae due to having a unique experiment system of quantitatively analyzing the ability to protect the flanking cell upon hypotonic shock- induced hyphal wounding1). Here, 776 genes were selected as multicellularity-specific uncharacterized by using BLAST-based genomic comparison between multicellular ascomycetes (A. oryzae, Aspergillus fumigatus and Aspergillus nidulans) and unicellular ascomycetes (Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida albicans) along with gene ontology category “no biological data available”. Proteins encoded by the genes were expressed as EGFP fusion in A. oryzae, and 8% of the proteins tested were found to localize to the septum. Approximately 40% of deletion strains lacking the septum-localizing proteins exhibited lower abilities to protect the flanking cell upon wounding. In conclusion, the present genomic comparison along with localization screening allowed us to successfully find many novel proteins having a role in fungal cell-to-cell communication. 1. Maruyama and Kitamoto (2019) The Mycota VIII, pp. 3-14 2. Analysis of self-assembly mechanism of hydrophobin RolA of Aspergillus oryzae using Langmuir- Blodgett method Keietsu Abe1, Yuki Terauchi1, Takumi Tanaka1, Masaya Mitsuishi1, Hiroshi Yabu2, ASPERFEST 17 9 Akira Yoshimi3, Fumihiko Hasegawa3 1 Graduate School of Agricultural Science, Department of Microbial Biotechnology, Sendai, Miyagi, Japan 2 Tohoku University, WPI-Advanced Institute for Materials Research, Sendai Miyagi Japan 3 Tohoku University, New Industry Creation Hatchery Center, Sendai Miyagi Japan Hydrophobins are small secreted amphipathic proteins produced by filamentous fungi and contain eight conserved cysteine residues that form four intramolecular disulfide bonds. Hydrophobins are classified into two classes (class I and class II) according to their amino acid sequence, hydropathy profile, and the solubility of their self-assembled films. Class I hydrophobins adsorb to solid surfaces and are localized at interfaces between hydrophobic and hydrophilic phases, resulting in formation of self- assembled structures so called “rodlets” which are similar to β-amyloid fibrils. Class I hydrophobin RolA produced by Aspergillus oryzae attaches to solid surfaces, recruits polyesterase CutL1, and consequently promotes hydrolysis of polyesters. Because several positively charged residues in the N-terminal region of RolA face to water-phase and are involved in the interaction with CutL1, the orientation of RolA molecule on the solid surface is important. However, the mechanism by which RolA forms the self- assembled structure remains unclear. Using the Langmuir technique, we analyzed the process in which RolA formed the self-assembled structure at the air-water interface. We also transferred the assembled RolA structures onto hydrophobic or hydrophilic silicon basal-plates and observed