Abietane and Pimarane Diterpene Acid Evolution in Scots Pine Pinus

Total Page:16

File Type:pdf, Size:1020Kb

Abietane and Pimarane Diterpene Acid Evolution in Scots Pine Pinus Abietane and pimarane diterpene acid evolution in Scots pine Pinus sylvestris needles in relation to feeding of the pine sawfly, Diprion pini L L Buratti, Jp Allais, C Geri, M Barbier To cite this version: L Buratti, Jp Allais, C Geri, M Barbier. Abietane and pimarane diterpene acid evolution in Scots pine Pinus sylvestris needles in relation to feeding of the pine sawfly, Diprion pini L. Annales des sciences forestières, INRA/EDP Sciences, 1990, 47 (2), pp.161-171. hal-00882698 HAL Id: hal-00882698 https://hal.archives-ouvertes.fr/hal-00882698 Submitted on 1 Jan 1990 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Original article Abietane and pimarane diterpene acid evolution in Scots pine Pinus sylvestris needles in relation to feeding of the pine sawfly, Diprion pini L. L Buratti JP Allais C Geri M Barbier 1 Institut de Chimie des Substances Naturelles - CNRS 91198 Gif-sur-Yvette, Cedex ; 2 Station de Zoologie Forestière - INRA Ardon 45160 Olivet, France (Received 16 February 1989; accepted 30 June 1989) Summary - Abietane and pimarane resin acids extracted from the needles of Scots pine, Pinus sylvestris, were analysed by reverse phase HPLC followed by GC of their methyl esters, in relation to the seasons, or the age of the trees. P sylvestris is the habitual host plant of the sawfly Diprion pini (Hymenoptera, Diprionidae) and results of the analyses were correlated with the feeding pattern of this insect in nature. An increase in resin acid concentration was observed during the growing season, but no direct relationship could be established with the feeding preferences of these insects. Young pines contained lower levels of abietane and pimarane diterpene acids than 10 or 30 year-old pines. Previous defoliation induced an increase in the neutral fraction and, although less so, in the diterpene acids in the needles formed the following year. The observed results are discussed in relation to the development of Diprion pini larvae and to previous hypotheses from other authors concerning the antifee- dant properties of the resin acids. It is concluded that, if the abietane and pimarane diterpene acids interfere with the biology of Diprion pini, they cannot, however, be considered as the most important factors in the natural equilibria of this species. Pinus sylvestris / Diprion pini / Diprionidae / sawfly / abietane and pimarane diter- pene acids / pine foliage / seasonal average variation / antifeedant property Résumé - Évolution des acides diterpéniques de types abiétique et pimarique dans le feuillage du pin sylvestre pinus sylvestris L ; impact de l’âge des aiguilles et des arbres, influence des défoliations passées. Conséquences pour le lophyre du pin Diprion pini L. Les acides résiniques de types abiétique et pimarique extraits des aiguilles du pin sylvestre, Pinus sylvestris, ont été analysés par HPLC sur phase inverse et CPG de leurs esters mé- thyliques, en fonction des saisons et de l’âge des arbres. P sylvestris est la plante hôte habituelle de Diprion pini (Hyménoptères, Diprionidés) et les résultats des analyses ont été corrélés à l’aptitude de cet insecte à se nourrir sur le feuillage de cet arbre. On note une augmentation du taux des acides résiniques à la belle saison, durant la phase de croissance des aiguilles, mais aucune relation directe n’a pu être observée pour expliquer les préférences alimentaires de cet insecte. Les jeunes pins contiennent un taux plus faible d’acides résini- ques de types abiétique et pimarique que les arbres âgés de 10 ou 20 ans. La défoliation Correspondence and reprints de l’arbre induit dans les aiguilles formées l’année suivante une augmentation du taux des- lipides neutres et, à moindre titre, des acides résiniques (plus particulièrement de l’acide abiétique). Les résultats obtenus sont discutés en fonction du choix alimentaire des larves de D pini et des hypothèses émises par d’autres auteurs concernant l’action anti-appétante des acides résiniques. En conclusion, l’aptitude de D pini à consommer le feuillage du pin sylvestre, Pinus sylvestris, ne paraît pas être directement liée aux variations de sa teneur en acides résiniques de types pimarique et abiétique. Toutefois ceci n’exclut pas toute action des ces composés dans la relation D pini - Pinus sylvestris comme le suggèrent certains résultats: taux particulièrement élevé de l’acide abiétique dans le feuillage des pins un an après une défoliation et dans les aiguilles des essences non attaquées tel Pinus pinaster. Seule une étude exhaustive des composés de la fraction acide, et de leur variations nous permettra d’apprécier le rôle effectif des acides résiniques dans les interactions Diprions-Pins sylvestres. Pinus sylvestris / Pinacée / Diprion pini / Diprionidé / Tenthrède / acide pimarique / acide abiétique / aiguille de pin / feuillage / variation saisonnière / propriété anti- appétante INTRODUCTION prion or Diprion species living on the Jack pine, Pinus banksiana, Lambert Scots pine, Pinus sylvestris L, is an (Schuh and Benjamin, 1984a, b); they economically important European pulp- were also tested on Pristiphora erich- wood and lumber conifer. Diprion pini sonii, Hartig larvae living on Larix lari- L (Hymenoptera, Diprionidae), a wide- cinia, Du Roi (K Koch) (Wagner et al, spread pine sawfly living in European 1983). All these authors concluded that coniferous forests, is able to cause resin acids could be the compounds af- serious damage to Scots pines during fecting sawfly larval development. its outbreaks. Thus, thousands of hec- If abietane and pimarane diterpere tares can be defoliated in less than acids interfere with larval mortality and 2 years (Dusaussoy and Geri, 1966; feeding behaviour in D pini, the quali- Eichhorn, 1982; Geri et al, 1982; Geri tative and quantitative evolutions of and Goussard, 1984; Geri, 1988). these compounds in the foliage should D pini does not feed on young, but be correlated to the habitual feeding on mature, foliage, as is the case for pattern of this insect. many Diprionidae and even other Previous analyses of Pinus sylvestris sawfly species (All and Benjamin, needle resin acids reported the pre- 1975a, b; All et al, 1975; Ikeda et al, sence of labdane diterpene acids such 1977a, b. Wagner et al, 1979; Niemela as manoyl oxid acid (Bardyshev et al, et al, 1982). As previously shown, 1981), pinificolic acid (Enzell and young foliage has a deterrent effect on Theander, 1962), dehydropinifolic acid sawflies and affects larval survival of D (Norin et al, 1971, 1980), 4-epi-imbri- pini (Geri et al, 1985, 1988). cataloic acid (Tobolski and Zinkel, Previous results suggested that resin 1982), and of classical pimarane and acids could be involved in the de- abietane diterpene acids such as pi- terrence of young foliage. Thus, maric, isopimaric, sandaracopimaric, abietane and pimarane diterpene acids abietic, palustric, levopimaric, dehy- were tested for antifeedant activity droabietic and neoabietic acids (Norin, against the larvae of various Neodi- 1972; Tobolski and Zinkel, 1982). This paper attempts to correlate the (Geri et al, 1985, 1987). Systematic temporal distribution of abietane and analyses were caried out on selected pimarane diterpene acids in extracts Scots pine foliage during a period from Scots pine needles with the ability ranging from June to September. of D pini to feed on the pine foliage. D pini lives preferably on old trees, Abietane and pimarane diterpene acids the young pines being attacked only were selected because they were usu- during outbreaks (Geri and Goussard, ally tested in sawfly feeding bioassays 1984; Geri, 1988). The same phenom- (Wagner et al, 1983; Schuh and Ben- enon was observed in Sweden on jamin, 1984a, b). Scots pine defoliated by Neodiprion sertifer, Geoff. (Larsson and Tenow, As a of these SOME BIOLOGICAL DATA 1984). consequence pre- vious observations, analyses were also carried out on the of trees of In France, attacks foliage Diprion pini chiefly different Pinus sylvestris but Pinus nigra Arnold ages. Furthermore, resistance ssp. laricio is also weakly damaged at long lasting the end of such outbreaks. P pinaster induced by defoliation (Haukioja and could Aiton is almost never attacked. Exotic Hakala, 1975; Haukioja, 1980) a crucial role in the of species such as P contorta Dougl and play collapse leaf feeder For P radiata D Don show some damage populations. example, (Geri, 1988). Diterpene acid analysis needle quality of Larix decidua re- mained low for defoliators 4 after was carried out on the mature foliage years a decrease in the of these species to find out if the dam- defoliation, inducing success of the insect dini- age could be correlated to pimarane Zeiraphera and abietane resin acid rates. ana Guenée (Benz, 1974; Baltensweiler et Fischlin and Baltensweiler, pini is usually bivoltine in the al, 1977; Diprion Quantitative resin acid Paris Basin. The first generation 1979). changes were shown to occur after wounds in develops from April to July. Eggs are Pinus sylvestris bark (Gref and Erics- laid as as mid-April and hatch be- early We found that the tween late May and early June. The son, 1984). develop- ment of D pini larvae feeding on new larvae feed on the foliage of the pre- of pines defoliated the previous vious years and their growth generally foliage was altered. We observed in ends at the beginning of July. At this year par- ticular a decrease in female time the larvae disperse and transform significant fecondity (Geri et al, 1988). Newly to which spin cocoons on eonymphs, formed Scots needles from trees or in the duff, and then pine vegetation defoliated either in the change successively into pronymphs, artificially pre- vious or by Diprion pini in the pupae, and adults.
Recommended publications
  • Forestry Department Food and Agriculture Organization of the United Nations
    Forestry Department Food and Agriculture Organization of the United Nations Forest Health & Biosecurity Working Papers OVERVIEW OF FOREST PESTS ROMANIA January 2007 Forest Resources Development Service Working Paper FBS/28E Forest Management Division FAO, Rome, Italy Forestry Department DISCLAIMER The aim of this document is to give an overview of the forest pest1 situation in Romania. It is not intended to be a comprehensive review. The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. © FAO 2007 1 Pest: Any species, strain or biotype of plant, animal or pathogenic agent injurious to plants or plant products (FAO, 2004). Overview of forest pests - Romania TABLE OF CONTENTS Introduction..................................................................................................................... 1 Forest pests and diseases................................................................................................. 1 Naturally regenerating forests..................................................................................... 1 Insects ..................................................................................................................... 1 Diseases................................................................................................................
    [Show full text]
  • And Its Glucoside in Pinus Sylvestris Needles Consumed by Diprion Pini Larvae
    Original article Quantitative variations of taxifolin and its glucoside in Pinus sylvestris needles consumed by Diprion pini larvae MA Auger C Jay-Allemand C Bastien C Geri 1 INRA, Station de Zoologie Forestière; 2 INRA, Station d’Amélioration des Arbres Forestiers, F-45160 Ardon, France (Received 22 March 1993; accepted 2 November 1993) Summary — The relationships between quantitative variations of 2 flavanonols in Scots pine needles and Diprion pini larvae mortality were studied. Those 2 compounds were characterized as taxifolin (T) and its glucoside (TG) after hydrolysis and analysis by TLC, HPLC and spectrophotometry. Quantitative differences between 30 clones were more important for TG than for T, nevertheless clones which presented a content of taxifolin higher than 1.5 mg g-1 DW showed a T/TG ratio equal to or greater than 0.5 (fig 2). Quantitative changes were also observed throughout the year. The amount of taxifolin peaked in autumn as those of its glucoside decreased (fig 3). Darkness also induced a gradual increase of T but no significant effect on TG (fig 4). Storage of twigs during feeding tests and insect defoliation both induced a strong glucosilation of taxifolin in needles (table I). High rates of mortality of Diprion pini larvae were associated with the presence of T and TG both in needles and faeces (table II). Preliminary experiments of feeding bioassay with needles supplemented by taxifolin showed a significant reduction of larval development but no direct effect on larval mortality (table III). Regulation processes between taxifolin and its glucoside, which could involve glucosidases and/or transferases, are discussed for the genetic and environmental factors studied.
    [Show full text]
  • The Pennsylvania State University
    The Pennsylvania State University The Graduate School PLANTS AS ILLEGITIMATE RECEIVERS OF INSECT SIGNALS: INSIGHT FROM MAIZE AND TEOSINTE A Thesis in Entomology by Julianne Golinski © 2020 Julianne Golinski Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science August 2020 ii The thesis of Julianne Golinski was reviewed and approved by the following: John F. Tooker Professor of Entomology and Extension Specialist Thesis Advisor Jared G. Ali Assistant Professor of Entomology Ben McGraw Associate Professor of Turfgrass Science Gary W. Felton Professor of Entomology Head of Department of Entomology iii ABSTRACT Plants have evolved to be sensitive to a range of insect-associated cues to detect the presence of herbivorous species and mount effective defense responses against future attack. Insect pheromones have recently been added to this library of cues as some plant species can act as “illegitimate receivers” that eavesdrop on the pheromones of insect herbivores, which act as reliable indicators of future larval damage. My thesis begins with a review that explores the perceptive capabilities of plants and how the ability to eavesdrop on insect pheromone cues may have evolved. I also cover the three known examples of plant illegitimate receivers and use shared traits in these systems to build criteria for finding other host plant species that may also utilize this strategy; native host plants species with (1) native, co-evolved insect herbivore species (2) that release abundant amounts of chemicals on or near the host plant, and (3) substantially reduce host plant fitness. My second chapter highlights two agriculturally relevant plant species that fit the criteria, maize (Zea mays ssp.
    [Show full text]
  • Plant Industry Annual Report
    New Hampshire Department of Agriculture, Markets & Food Shawn N. Jasper, Commissioner New Hampshire Department of Agriculture, Markets & Division of Plant Report to the Eastern Plant Summary of 2020 Nursery program https://www.agriculture.nh.gov/divisions/plant-industry/nursery-plant-dealers.htm The New Hampshire Plant Dealer license encompasses growers, wholesalers, and retailers of nursery and florists stock. Anyone engaged in the sale or transfer of rooted plants is required to have a Plant Dealer license. Table 1: Licensees and nursery inspections: 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 Licensed plant dealers 700 725 746 722 736 782 818 831 828 829 Newly licensed plant dealers 53 50 13 24 64 70 29 45 38 43 Exporting nurseries 65 65 65 65 65 65 65 70 51 79 Nursery inspections 152 119 120 123 248 181 135 179 315 456 Despite a late start in the season for nursery inspections due to COVID19 restrictions in the state, the nursery inspection season was active with inspections at box stores, grocery stores, and other retail outlets continuing past the peak plant sales season. Out of the 456 visits to New Hampshire nurseries, greenhouses, landscapers, and other plant dealers, 83 of the inspections focused on the CAPS nursery pests, and an additional 132 inspections were focused on larger nurseries and out-of-state shippers. Quality pests detected during inspection were: aphids, lily leaf beetle, powdery mildew, thrips, whitefly, elongate hemlock scale, mealybug, daylily leaf miner, leaf tier, woolly bark aphid, wooly adelgid, scale, cabbage worm, fall web worm, red humped caterpillar, boxwood volutella blight, Colorado potato beetle, and weeds.
    [Show full text]
  • Abietane and Pimarane Diterpene Acid Evolution to Feeding of the Pine
    Original article Abietane and pimarane diterpene acid evolution in Scots pine Pinus sylvestris needles in relation to feeding of the pine sawfly, Diprion pini L. L Buratti JP Allais C Geri M Barbier 1 Institut de Chimie des Substances Naturelles - CNRS 91198 Gif-sur-Yvette, Cedex ; 2 Station de Zoologie Forestière - INRA Ardon 45160 Olivet, France (Received 16 February 1989; accepted 30 June 1989) Summary - Abietane and pimarane resin acids extracted from the needles of Scots pine, Pinus sylvestris, were analysed by reverse phase HPLC followed by GC of their methyl esters, in relation to the seasons, or the age of the trees. P sylvestris is the habitual host plant of the sawfly Diprion pini (Hymenoptera, Diprionidae) and results of the analyses were correlated with the feeding pattern of this insect in nature. An increase in resin acid concentration was observed during the growing season, but no direct relationship could be established with the feeding preferences of these insects. Young pines contained lower levels of abietane and pimarane diterpene acids than 10 or 30 year-old pines. Previous defoliation induced an increase in the neutral fraction and, although less so, in the diterpene acids in the needles formed the following year. The observed results are discussed in relation to the development of Diprion pini larvae and to previous hypotheses from other authors concerning the antifee- dant properties of the resin acids. It is concluded that, if the abietane and pimarane diterpene acids interfere with the biology of Diprion pini, they cannot, however, be considered as the most important factors in the natural equilibria of this species.
    [Show full text]
  • Emerging Issues for Biodiversity Conservation in a Changing Climate
    Secretariat of the CBD Technical Series No. 29 Convention on Biological Diversity Emerging Issues for Biodiversity Conservation in a Changing Climate Abstracts of Poster Presentations at the 12th Meeting of the Subsidiary Body on 29Scientific, Technical and Technological Advice of the Convention on Biological Diversity 2–6 July 2007 in Paris, France CBD Technical Series No. 29 Emerging Issues for Biodiversity Conservation in a Changing Climate Abstracts of Poster Presentations at the 12th Meeting of the Subsidiary Body on Scientific, Technical and Technological Advice of the Convention on Biological Diversity 2–6 July 2007 in Paris, France Published by the Secretariat of the Convention on Biological Diversity. ISBN: 92-9225-073-6 Copyright © 2007, Secretariat of the Convention on Biological Diversity The designations employed and the presentation of material in this publication do not imply the expres- sion of any opinion whatsoever on the part of the Secretariat of the Convention on Biological Diversity concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views reported in this publication do not necessarily represent those of the Convention on Biological Diversity nor those of the reviewers. This publication may be reproduced for educational or non-profit purposes without special permission from the copyright holders, provided acknowledgement of the source is made. The Secretariat of the Convention would appreciate receiving a copy of any publications that uses this document as a source. Citation Secretariat of the Convention on Biological Diversity (2007). EMERGING ISSUES FOR BIODIVERSITY CONSERVATION IN A CHANGING CLIMATE.
    [Show full text]
  • Defense of Scots Pine Against Sawfly Eggs (Diprion Pini) Is Primed by Exposure to Sawfly Sex Pheromones
    Defense of Scots pine against sawfly eggs (Diprion pini) is primed by exposure to sawfly sex pheromones Norbert Bittnera, Janik Hundackera, Ander Achotegui-Castellsb,c, Olle Anderbrantd, and Monika Hilkera,1 aDahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 12163 Berlin, Germany; bCentre de Recerca Ecològica i Aplicacions Forestals (CREAF), Barcelona, 08193 Catalonia, Spain; cGlobal Ecology Unit, CREAF-Consejo Superior de Investigaciones Científicas, Universitat Autònoma de Barcelona, Barcelona, 08193 Catalonia, Spain; and dDepartment of Biology, Lund University, 223 62 Lund, Sweden Edited by James H. Tumlinson, Pennsylvania State University, University Park, PA, and approved October 30, 2019 (received for review June 26, 2019) Plants respond to insect infestation with defenses targeting insect egg deposition (16). In addition, many plant species have been eggs on their leaves and the feeding insects. Upon perceiving cues shown to change their leaf odor in response to insect egg de- indicating imminent herbivory, such as damage-induced leaf odors position; the egg-induced leaf odor attracts parasitic wasps that emitted by neighboring plants, they are able to prime their defenses kill the eggs (17). Since insect mating precedes egg deposition, against feeding insects. Yet it remains unknown whether plants can cues like insect sex pheromones might serve as reliable stimuli amplify their defenses against insect eggs by responding to cues indicative of imminent egg deposition, thus eliciting plant re- indicating imminent egg deposition. Here, we tested the hypothesis sponses harming the eggs. that a plant strengthens its defenses against insect eggs by respond- Here we present a study testing the hypothesis that exposure ing to insect sex pheromones.
    [Show full text]
  • Pupation Site Selection and Enemy Avoidance in the Introduced Pine Sawfly (Diprion Similis)
    2017 Winter Ecology:Northeastern Insights from Naturalist Biology and History Vol. 24, Special Issue 7 2017 N.T.Northeastern Wheelwright, Naturalist et al. 24(Special Issue 7):B19–B31 Pupation Site Selection and Enemy Avoidance in the Introduced Pine Sawfly (Diprion similis) Nathaniel T. Wheelwright1,*, Liam U. Taylor1, Benjamin M. West1, Erin R. Voss1, Sabine Y. Berzins1, Andrew R. Villeneuve1, Hannah R. LeBlanc1, Victor B. Leos1, Samuel J. Mayne1, Sarah A. McCarthy1, Shan J. Nagar1, and Jenna S. Watling1 Abstract - Insects that pupate on the branches of trees and shrubs suffer mortality from both predators and parasitic wasps. Which natural enemy represents the greater threat and there- fore the stronger selection force on pupation site selection depends upon the time of year, the relative abundance of predators versus parasitoids, and the availability of alternative prey or hosts. Predation by foraging birds and mammals is likely to occur most commonly in winter when leaves have fallen, cocoons are conspicuous, and higher quality prey are scarcer. Inaccessibility and crypsis of pupation sites may provide protection from visu- ally hunting predators. Attacks by parasitic wasps, which take place only during warmer months, may not be as easily avoided by inaccessibility or crypsis. We studied the patterns and mortality risks of pupation site selection in Diprion similis (Hymenoptera: Diprionidae; Introduced Pine Sawfly). Cocoons that were smaller than average and situated in relatively inaccessible sites (thinner branches, underside of branches) were less likely to be attacked by predators; background matching in terms of branch size proved not to improve survival. In contrast, the probability that a cocoon would be attacked by parasitic wasps (primarily Monodontomerus dentipes; Hymenoptera: Torymidae) was unaffected by location along branches, indicating that parasitoids are more difficult to escape through pupation site selection.
    [Show full text]
  • Annex 1. Pest Species Distribution in the Selected Countries, by Region
    Annex 1: Pest species distribution in the selected countries, by region 193 Annex 1 Pest species distribution in the selected countries, by region AFRICA Order/phylum: South Pest species Ghana Kenya Malawi Mauritius Morocco Sudan family Africa Insects Amyna punctum Lepidoptera: DNC Noctuidae Anacridum Orthoptera: DNB melanorhodon Acrididae Analeptes trifasciata Coleoptera: DNB Cerambycidae Anaphe panda Lepidoptera: DNB Thaumetopoeidae Anaphe venata Lepidoptera: DNB Notodontidae Ancistrotermes Isoptera: DNB latinotus Termitidae Apate indistincta Coleoptera: DPB Bostrichidae Apate monachus Coleoptera: DPB DPB Bostrichidae Apate terebrans Coleoptera: DPB Bostrichidae Bruchidius uberatus Coleoptera: DPB Bruchidae Buzura abruptaria Lepidoptera: DNC Geometridae Caryedon serratus Coleoptera: DPB Bruchidae Chrysobothris Coleoptera: TPB dorsata Buprestidae Cinara cronartii Hemiptera: TPC Aphididae Cinara cupressivora Hemiptera: TPC TPC TPC Aphididae Cinara pinivora Hemiptera: TPC Aphididae Cisseis rufobasalis Coleoptera: DNB Buprestidae Coryphodema tristis Lepidoptera: DPB Cossidae T= Introduced pest P= Planted forest B= Broadleaf trees D= Indigenous pest N= Naturally regenerated forest C= Coniferous trees 194 Global review of forest pests and diseases Order/phylum: South Pest species Ghana Kenya Malawi Mauritius Morocco Sudan family Africa Cubitermes spp. Isoptera: DNB Termitidae Diclidophlebia Hemiptera: DPB eastopi Psyllidae Distantiella Hemiptera: DPB theobroma Miridae Dysmicoccus brevipes Hemiptera: DPB Pseudococcidae Ellimenistes laesicollis
    [Show full text]
  • Biological Control of Pine Sawfly (Diprion Pini L.) and Molecular Characterisation of Effective Strains
    Romanian Biotechnological Letters Vol. 21, No. 2, 2016 Copyright © 2016 University of Bucharest Printed in Romania. All rights reserved ORIGINAL PAPER Biological Control of Pine Sawfly (Diprion pini L.) and Molecular Characterisation of Effective Strains Received for publication, January 24, 2015 Accepted, February 25, 2015 FATIH DADASOGLU1,, GOKSEL TOZLU2, RECEP KOTAN2, TEMEL GOKTURK3 , KENAN KARAGOZ1,4 1Faculty of Science and Letters, Department of Molecular Biology and Genetics, Ağrı İbrahim Çeçen University Ağrı, Turkey 2 Faculty of Agriculture, Department of Plant Protection, Ataturk University, Erzurum, Turkey 3Faculty of Forestry, Department of Forest Engineering, Artvin Coruh University, Artvin, Turkey 4Central Research and Application Laboratory, Agri Ibrahim Cecen University, Ağrı, Turkey *Correspondence address: Faculty of Science and Letters, Department of Molecular Biology and Genetics, Agri Ibrahim Cecen University Ağrı, Turkey e-mail: [email protected]; Tel: +90 0472 215 4073; Fax: +90 0472 215 6554 Abstract Diprion pini is one of the most important pests in forest trees around the world. Also the pest was determined in pine forests around the Turkey, causing outbreaks in some regions. Therefore, this study aimed to develop bacterial biopesticides, which can be used to control Diprion pini remarkably and at the same time which do not threaten the environment and human health. Bacterial strains which were effective against different pests in some studies were used. Their insecticidal effects were tested on larvae of Diprion pini in vitro on petri dishes. Seven days after applications, some of the tested strains showed more or less insecticidal activity against this pest. The highest activity was obtained for strains FD-17 and FD-1causing 66.7-80% mortality, respectively.
    [Show full text]
  • Pine Sawfly (Diprion Pini L.) – Related Changes in Scots Pine Crown Defoliation and Possibilities of Recovery
    Polish J. of Environ. Stud. Vol. 16, No. 3 (2007), 363-369 Original Research Pine Sawfly (Diprion pini L.) – Related Changes in Scots Pine Crown Defoliation and Possibilities of Recovery A. Augustaitis* lithuanian university of Agriculture, Forest monitoring laboratory, studentų 13, lT-53362 Kaunas, Lithuania Received: April 19, 2006 Accepted: November 30, 2006 Abstract recently, outbreaks of pine sawfly (Diprion pini [l.]) became one of the most relevant stresses resulting in widespread deterioration of scots pine (Pinus sylvestris [l.]) conditions in central and Northern europe. At the beginning of the 1990s lithuania experienced the largest outbreak of Diprion pini L. Field studies were carried out in middle-aged pine stands, exhibiting different degrees of dam- age caused by pine sawfly, in order to examine the resultant changes in scots pine defoliation and death probability. “mirror effect” in defoliation changes from tree decline to recovery was detected. if in the period of pest outbreak the largest pine trees, crown condition of which had been better than that of the other, suffered the most serious needle losses, then in the period of recovery these trees re- covered most intensively. Intensity of tree mortality was in relation to stands mean defoliation degree. however, the relative diameter of dead trees did not differ among stands significantly (p>0.05) as suppressed trees died first. No threshold for crown defoliation, exceedance of which would inevitably result in Scots pine tree death, was found. Keywords: Diprion pini, crown defoliation, crown recovery, tree mortality Introduction currently outbreaks of common pine sawfly (Diprioni- dae) have to be regarded as a significant threat to pine forests, Recently considerable attention has been paid to the not only because of the increase in affected areas, but also study of natural stresses contributing to changes in forest because of their high damage potential [8].
    [Show full text]
  • (Coleoptera: Buprestidae) Y Dociostaurus Maroccanus (Orthoptera: Acrididae), Dos Insectos Plaga De La Península Ibérica
    Comunicación química intraespecífica de Coroebus spp. (Coleoptera: Buprestidae) y Dociostaurus maroccanus (Orthoptera: Acrididae), dos insectos plaga de la Península Ibérica Benjamin Fürstenau ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tdx.cat) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR (www.tdx.cat) ha sido autorizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR. No se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al resumen de presentación de la tesis como a sus contenidos.
    [Show full text]