UBE2I Antibody Cat. No.: 45-164

UBE2I Antibody

45-164 (0.3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA 45-164 (0.3ug/ml) staining of Rat Ovary lysate (35ug buffer pH9, HRP-staining. in RIPA buffer). Detected by chemiluminescence.

Specifications

HOST SPECIES: Goat

SPECIES REACTIVITY: Human, Rat

HOMOLOGY: Expected Species Reactivity based on : Mouse, Dog

IMMUNOGEN: The immunogen for this antibody is: SGIALSRLAQERK-C

TESTED APPLICATIONS: ELISA, IHC, WB

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 17kDa band observed in Human Kidney lysates and in Rat Ovary, Heart and Testis lysates (calculated MW of 18.0kDa according to Human NP_003336.1). Recommended APPLICATIONS: concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration: 1ug/ml.

September 23, 2021 1 https://www.prosci-inc.com/ube2i-antibody-45-164.html Reported variants represent identical protein: NP_919237.1, NP_003336.1, NP_919236.1, SPECIFICITY: NP_919235.1

POSITIVE CONTROL: 1) Cat. No. 1305 - Human Kidney Tissue Lysate

Properties

Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

CONCENTRATION: 500 ug/mL

STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.

Additional Info

OFFICIAL SYMBOL: UBE2I

UBE2I, -conjugating E2I (UBC9 homolog, yeast), C358B7.1, P18, UBC9, SUMO-1-protein ligase, ubiquitin carrier protein, ubiquitin conjugating enzyme 9, ALTERNATE NAMES: ubiquitin-conjugating enzyme E2I, ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating enzyme UbcE2A, ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitin-protein ligase E2I, OTTHUMP00000198126, OTTHUMP00000198127

ACCESSION NO.: NP_003336.1

PROTEIN GI NO.: 4507785

GENE ID: 7329

Background and References

1) Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, REFERENCES: Shenk T, Deuel TF. Molecular cloning of the cDNA and localization of the for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996 Oct 4;271(40):24811-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 23, 2021 2 https://www.prosci-inc.com/ube2i-antibody-45-164.html