UBE2I Antibody Cat. No.: 45-164
UBE2I Antibody
45-164 (0.3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA 45-164 (0.3ug/ml) staining of Rat Ovary lysate (35ug buffer pH9, HRP-staining. protein in RIPA buffer). Detected by chemiluminescence.
Specifications
HOST SPECIES: Goat
SPECIES REACTIVITY: Human, Rat
HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Dog
IMMUNOGEN: The immunogen for this antibody is: SGIALSRLAQERK-C
TESTED APPLICATIONS: ELISA, IHC, WB
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 17kDa band observed in Human Kidney lysates and in Rat Ovary, Heart and Testis lysates (calculated MW of 18.0kDa according to Human NP_003336.1). Recommended APPLICATIONS: concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration: 1ug/ml.
September 23, 2021 1 https://www.prosci-inc.com/ube2i-antibody-45-164.html Reported variants represent identical protein: NP_919237.1, NP_003336.1, NP_919236.1, SPECIFICITY: NP_919235.1
POSITIVE CONTROL: 1) Cat. No. 1305 - Human Kidney Tissue Lysate
Properties
Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION: 500 ug/mL
STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.
Additional Info
OFFICIAL SYMBOL: UBE2I
UBE2I, ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), C358B7.1, P18, UBC9, SUMO-1-protein ligase, ubiquitin carrier protein, ubiquitin conjugating enzyme 9, ALTERNATE NAMES: ubiquitin-conjugating enzyme E2I, ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating enzyme UbcE2A, ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitin-protein ligase E2I, OTTHUMP00000198126, OTTHUMP00000198127
ACCESSION NO.: NP_003336.1
PROTEIN GI NO.: 4507785
GENE ID: 7329
Background and References
1) Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, REFERENCES: Shenk T, Deuel TF. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996 Oct 4;271(40):24811-6.
ANTIBODIES FOR RESEARCH USE ONLY.
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September 23, 2021 2 https://www.prosci-inc.com/ube2i-antibody-45-164.html