Concentrations of Some Heavy Metals in Water, Sediment, and Some
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Internatıonal Journal of Natural and Engineering Sciences 7 (3): 56-62, 2013 ISSN: 1307-1149, E-ISSN: 2146-0086, www.nobel.gen.tr Concentrations of some Heavy Metals in Water, Sediment and some Living Organisms from the Zamanti River in Turkey Didem AYDIN1 Omer Faruk COSKUN2* 1Erciyes University, Faculty of Sciences, Department of Biology 38039, Kayseri, Turkey 2Erciyes University, Institute of Science 38039, Kayseri, Turkey *Corresponding author: Received: October 15, 2013 E-mail: [email protected] Accepted: December 12, 2013 Abstract Water samples, sediments, aquatic plant (Nasturtium officinale) and fish species (Oncorhynchus mykiss, Cyprinus carpio) from the Zamantı River of Turkey were analyzed quantitatively for the presence of chromium, manganese and cadmium using an Atomic Absorption Spectrophotometer. The samples were collected from three stations. Water quality parameters, such as temperature, pH, dissolved oxygen and electrical conductivity were measured. According to the findings; pH value varied between 8.15-8.6. The gradual order of metals in water, sediment, Nasturtium officinale and fish species were given as follow; Mn>Cr>Cd in water, Mn>Cr>Cd in sediment, Mn>Cr>Cd in Nasturtium officinale , Mn>Cr>Cd in fish species. In comparison to international standards, low Cd and Cr levels were identified in all of the water, sediment, plant and fish samples. Mn levels were also low in the sediment, plant and fish samples. Only in the water samples were the Mn levels higher in comparison to the EC (Europian Community, 1998) and EPA (Environment Protection Agency, 2002) standard. Keywords: Cyprinus carpio, heavy metals, Nasturtium officinale, Oncorhynchus mykiss, Zamantı River. INTRODUCTION sediments is important for determining these metals’ environmental effects on local communities. In rivers, a correlation is observed between the concentration of heavy Pollutants and environmental pollution can lead to metals in the bottom sediments and the concentration of significant problems for both living organisms and heavy metals in water [11]. societies. The increases in worldwide population, as well as Fish are in important source of food for humans. They the advances in technology, contribute significantly to represent a rich source of protein, polyunsaturated fatty environmental pollution [1]. In our day, pollutants are acids (particularly omega-3 fatty acids), calcium, zinc (Zn) being constantly being released into the air, soil, and iron (Fe) [12]. Fish require certain metals to maintain underground water sources, lakes and rivers [2, 3]. As a and sustain their normal metabolic functions [13]. type of pollutant, heavy metals can accumulate in aquatic However, excessive intake of these metals can also lead to environments and lead to significant toxic effects [4]. In toxic effects. Heavy metals released as a result of various recent years, there has been a considerable rise in the physicochemical processes can be taken up by other number of problems associated with heavy metal pollution organisms through the food chain [3, 14]. Fish samples are in aquatic environments. Even small quantities of heavy one of the most commonly used indicators for freshwater metals can contribute significantly to environmental systems, and are employed to estimate and identify the pollution. levels of trace element pollution [15]. For this reason, Heavy metals can be classified as toxic, semi-essential numerous studies have been published on the measurement and essential [5]. Essential heavy metals can cause toxic of heavy metals in fish [10, 16-18]. effects in organisms when present in excessive quantities All metal ions, even those required for essential [6]. The concentration of certain metals in aquatic functions in plants, are extremely toxic to the metabolism environments can increase due to the release of of living organisms if taken in excessive quantities [19]. agricultural, domestic and industrial wastes into water Metals can lead to oxidative stress in plants through the sources. Suspended or dissolved heavy metals and other formation of free radicals [20]. Plants posses various pollutants can be taken up by living organisms, or they can tolerance and resistance mechanisms against metal ions precipitate and accumulate in sediments. Most lakes, rivers [21, 22]. Every plant species demonstrates a different type and ocean sediments have been contaminated with of tolerance to different pollutants [23]. Aquatic pollutants. In the past ten years, the number of studies marcophytes accumulate heavy metals in their tissues. performed to demonstrate the environmental effects of Many studies have been conducted regarding the heavy metals in river and lake fish and sediments have accumulation of metals in aquatic plants [24-28]. For this increased [7- 10]. The distribution and levels of metals in reason, these plants can potentially be used for the sediment environments neighboring residential areas can biological filtering and treatment of certain metals in water provide evidence on the effect of humans on aquatic [29]. environments. Assessing the accumulation of metals in D. Aydin and O. F. Coskun / IJNES, 7 (3): 56-62, 2013 57 The aim of this study was to determine the concentration The branchia tissues were dissected using pincers and of certain heavy metals in water, sediment, plant scalpel, then 0.5 g of each was taken by weighing them on (Nasturtium officinale) and fish (Oncorhynchus mykiss and an analytical balance and put into microwave. They were Cyprinus carpio) samples from the Zamantı River, and to left to cool down the solvation process and transferred into provide information regarding the heavy metal levels of volumetric flasks after dilution. The prepared samples were these samples. Furthermore, this study will allow us to analyzed using the ICP-OES device. obtain information on the overall health of the The presence or absence of significant differences abovementioned organisms in this river ecosystem. between the Mn, Cd and Cr levels measured in Plant-root, Plant-body, water, sediment and fish samples from MATERIALS AND METHODS different stations was tested with the Kruskal Wallis test, which is a nonparametric test. For each heavy metal parameter, the mean and standard deviation of triplicate The headwater of the Zamanti River, which is the measurements was calculated. All statistical analyses were subject of the study, is located in the village of Serefiye, performed using SPSS 15.0 statistical software. The results Uzunyayla in the Pınarbası district of the province of were considered significant in the case of p < 0.05. Kayseri, at an elevation of 1637 meters. The Zamantı River then unifies with the Göksu River at about 80 km from Adana and they form the Seyhan River, which flows into RESULTS AND DISCUSSION the Mediterranean Sea. The Seyhan River is 560 km long 2 and its basin area is 20,100 km . The Zamantı River is Certain physical parameters of the Zamantı River were made up of quite a number of creeks and brooks. The part measured at three different stations; a comparative of the Zamanti River examined in this study is between the evaluation of these physical parameters is shown in Table location of its headwaters (Serefiye Village) and Bahcelik 1. The average water temperature of the Zamanti River Dam Lake (Pınarbaşı), where it flows first. Three stations during the course of the study was dedected as 8.6 oC. The equally distributed in the specified area were determined highest temperature was measured at the 1st station (10.1 during the study. oC); and the lowest temperature was measured at 2nd Some environmental parameters were studied at each station (6.2 oC). The pH values did not differ much among station during the field study using a multiparameter the stations and were determined to be 8.49 on the average. device. The water samples were taken in accordance with The lowest dissolved oxygen levels were measured at the the sampling rules from the stations located on the river 1st station (86 %) and the highest dissolved oxygen levels [30]. The pH, temperature, dissolved oxygen and electrical were measured at the 2nd station (108 %). The electrical conductivity values of the water samples were measured in conductivity values were measured to be 340, 438, and 440 the field using a WTW Multi 3400i device. microsiemens/cm at the 1st, 2nd, and 3rd stations respectively The water samples that were brought to the laboratory (Table 1). The results acquired from some environmental were filtered using a membrane filter of 0.45 μm pore parameters of the Zamanti River are shown in Table 1. diameter in order to detect the dissolved elements. The Although the pH values differ according to some water samples taken from the filtrate was buffered using environmental parameters measured at all of the stations, nitric acid. An appropriate volume of nitric acid was added. the water was found to be 1st class quality in terms of The sample was prepared for analysis by sealing and temperature and dissolved oxygen levels. However, the mixing the tube. The contents of the dissolved elements lightly alkali nature of the water stems mostly from the were measured using an ICP-OES device in compliance presence of bicarbonate ions. The temperature with relevant standards. measurements were found to be low. Since the water The sediment samples were put into colored glass boxes. temperature is significant in fish life, the fish were After the water was completely removed from the samples classified as cold water, mild and warm water fish [33]. The using a drying oven, 1 g was taken from each sample and water from the river provides optimum conditions for cold put into microwave solvation tubes. 5 mL HNO3 and 1 mL water fish. The dissolved oxygen values were also found to H2O2 was added on top of each tube and solvated in a be the lowest at the stations where the measured microwave oven. After this process, the tubes were cooled temperature was found to be the highest. However, the to room temperature and the solution in the tubes was dissolved oxygen concentration measured in the surface transferred into 25 mL polypropylene volumetric flasks.