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GenScript Your Innovation Partner in Drug Discovery! Service GenScript The Biology CRO Biological Service Guide
Bio-Reagent Center Contact Us Gene Services Protein Services
GenScript is dedicated to serving pharmaceutical, biotech, and academic customers in the areas of drug discovery, development, GenScript Biological Service Guide Peptide Services and life science research. With a global presence and local access, GenScript provides high-quality biology CRO services and Antibody Services around-the-clock account support to our customers all over the world. Contact us any time. Cell Line Services
Bio-Assay Center Contact: Lead Optimization Center All Correspondence Email: [email protected] GenScript USA Inc. Tel: 1-877-436-7274 (Toll-Free) 120 Centennial Ave. Tel: 1-732-885-9188, 1-732-885-9688 Antibody Drug Piscataway, NJ 08854 Fax: 1-732-210-0262, 1-732-885-5878 Development Center U.S.A.
Business Development: Email: [email protected] Tel: 1-732-317-5088
Regional Numbers: Australia: 1-800-172-605 (Toll-Free) Japan: 81-3-6206-8985 Belgium: 32-27-470-921 Netherlands: 31-20-890-5456 2010–2011 Denmark: 8088-7617 (Toll-Free) Norway: 800-19-172 (Toll-Free) France: 08-05-10-22-28 (Toll-Free) Portugal: 800-815-627 (Toll-Free) Finland: 0800-913-020 (Toll-Free) Singapore: 800-120-4549 (Toll-Free) Germany: 0-800-0006-320 (Toll-Free) Spain: 800-098-920 (Toll-Free) Hong Kong: 800-964-684 (Toll-Free) Sweden: 0200-884-573 (Toll-Free) Ireland: 1800-932-547 (Toll-Free) Switzerland: 0800-369-254 (Toll-Free) Israel: 1-809-216-068 (Toll-Free) United Kingdom: 08082-380203 (Toll-Free) 2010–2011
CALL NOW FOR MORE INFORMATION GenScript 1-732-885-9188 The Biology CRO Date Version: 05/14/2010 Fax: 1-732-210-0262 Web: www.genscript.com Email: [email protected] Your Innovation Partner in Drug Discovery! Table of Contents
Service Overview ...... 1
Bio-Reagent Center
Molecular Biology Services Gene Synthesis ...... 10 Mutagenesis Services ...... 15 Mutant Libraries (Protein Engineering) ...... 17 PCR Cloning and Subcloning Services ...... 19 Custom Plasmid DNA Preparation Services ...... 20 Custom ORF Cloning Services ...... 22 Custom Vector Construction Services...... 23 Knock-in and Knock-out Vector Construction Services ...... 24 GenScript USA Inc. siRNA and miRNA Services...... 25 Vector-Based siRNA Services Vector-Based miRNA Services Large-scale DNA Sequencing Services ...... 28 CloneArchTM Clone Storage and Management Center ...... 30
Custom Protein Services Bacterial Expression System ...... 33 Yeast Expression System ...... 36 Insect Cell Expression System ...... 38 Mammalian Cell Expression System ...... 40 Large-scale Protein Production Service...... 42 Protein Purification and Characterization ...... 44
Custom Peptide Services Standard Peptide Synthesis ...... 46 Peptide Modifications ...... 47 Custom Peptide Library Services ...... 48 Cosmetic Peptide Services...... 51 Click Peptide Service ...... 52 Custom Recombinant Peptides ...... 53 cGMP Peptide Synthesis ...... 54 Peptide Purity and Application ...... 56
Email: [email protected] Tel: 1-732-885-9188 - i - Table of Contents Table of Contents
Bio-Reagent Center Bio-Assay Center
Custom Antibody Services GPCR Assay Services ...... 90 Custom Polyclonal Antibody Services ...... 58 Ion Channel Assay Services ...... 92 Custom Polyclonal Antibody Services (Rabbit)...... 59 Kinase Assay Services ...... 94 Custom Polyclonal Antibody Services (Goat) ...... 62 Protease Assay Services ...... 95 Custom Polyclonal Antibody Services (Mouse) ...... 63 Miscellaneous Enzyme Assay Services ...... 97 Custom Polyclonal Antibody Services (Rat) ...... 64 Cardiac Safety Testing Services...... 99 Custom Polyclonal Antibody Services (Chicken)...... 65 One-stop Assay Services ...... 100 Western-GUARANTEED Antibody Service ...... 67 Custom Monoclonal Antibody Services ...... 68 Custom Monoclonal Antibody Services (Mouse/Rat) Lead Optimization Center Customized Monoclonal Antibody Services Monoclonal Antibody Production Service ...... 70 Medicinal Chemistry...... 103 Ascites Production Services Discovery Biology ...... 104 Roller Bottle Cell Culture Services Express Antibody Services ...... 72 MonoExpressTM Antibody Services Antibody Drug Development Center Express Rabbit Polyclonal Antibody Services Immunochemistry Services ...... 74 Antibody Humanization ...... 107 Antibody Purification Services Affinity Maturation ...... 107 Antibody Modification Services Stable Cell Line Development and Antibody Production ...... 107 Additional Services Supporting Services ...... 108 Large-scale Antibody Production Services ...... 76 Immunoassay Development Services ...... 77
Custom Cell Line Services Stable Cell Line Services for Protein and Monoclonal Antibody Production...... 79 Custom Stable Cell Line Services for Assay Development ...... 81 Transient Gene Expression Service ...... 82 Process Development and Transfer ...... 83 Catalog Stable Cell Lines ...... 83 Ready-to-UseTM Frozen Cells ...... 85
- ii - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - iii - GenScript Service Overview GenScript—Your Innovation Partner in Drug Discovery!
Custom Protein Services Molecular Biology Services Custom Antibody Services ● Bacterial Expression System ● Custom Polyclonal Antibody Services ● Gene Synthesis ● Yeast Expression System ● Custom Monoclonal Antibody Services ● Mutagenesis Services ● Insect Cell Expression System ● Monoclonal Antibody Production Service ● Mutant Libraries (Protein Engineering) ● Mammalian Cell Expression System ● Express Antibody Services ● PCR Cloning and Subcloning Services ● Large-scale Protein Production Service ● Immunochemistry Services ● Custom Plasmid DNA Preparation Bio-Reagent Services ● Protein Purification and Characterization ● Large-scale Antibody Production Services Services ● ● Custom ORF Cloning Services Immunoassay Development Services ● Custom Vector Construction Services Custom Peptide Services Custom Cell Line Services ● Knock-in and Knock-out Vector Construction Services ● Standard Peptide Synthesis ● Stable Cell Line Services for Protein and ● Monoclonal Antibody Production ● siRNA and miRNA Services Peptide Modifications ● ● Custom Stable Cell Line Services for Assay ● Large-scale DNA Sequencing Services Custom Peptide Library Services ● Development ● CloneArchTM Clone Storage and Cosmetic Peptide Services ● Transient Gene Expression Service Management Center ● Mirror-image Peptide Services ● Process Development and Transfer ● Custom Recombinant Peptides ● Catalog Stable Cell Lines ● cGMP Peptide Synthesis ● Ready-to-UseTM Frozen Cells ● Peptide Purity and Application Primary Secondary Assay Assay GPCR Assay Services Bio-Reagent Ion Channel Assay Services Kinase Assay Services Bio-Assay Protease Assay Services Compound Center Specificity Miscellaneous Enzyme Assay Services Screening Assay Cardiac Safety Testing Services Antibody Drug Lead HTS One-stop Assay Services Development Optimization Conversion
Bio-Assay GenScript—Your Innovation Partner in Drug Discovery! GenScript—Your Medicinal Chemistry Medicinal Lead Discovery Discovery Biology
Chemistry Optimization Biology Se rvi ce
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Antibody Affinity Humanization Maturation Antibody Humanization Antibody Drug Affinity Maturation Development Stable Cell Line Development and Antibody Production Supporting Services Bioprocess and Antibody Production
- 1 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 2 - Global Services, Local Access Selected Publications Global Services, Local Access
A total of 585 research articles have been published between Peptide 2004–2009, which cite GenScript’s services and products. • Dazhi Lai, Mimi Wan, Jie Wu, Paula Preston-Hurlburt, Ritu Kushwah, Thomas Grundström, Anthony N. Imbalzano, and Gene Tian Chi. Induction of TLR4-target genes entails • Sean Chapman, Christine Faulkner, Eirini Kaiserli, Carlos calcium/calmodulin-dependent regulation of chromatin Garcia-Mata, Eugene I. Savenkov, Alison G. Roberts, Karl J. remodeling. PNAS. Jan 2009; 106(4): 1169–1174. Oparka, and John M. Christie. The photoreversible Piscataway, NJ, U.S. Nanjing, fluorescent protein iLOV outperforms GFP as a reporter of • Slofstra SH, Bijlsma MF, Groot AP, Reitsma PH, Lindhout T, (Headquarters) China plant virus infection. PNAS. Dec 2008; 105(50): Ten Cate H, Spek CA. Protease-activated receptor-4 20038–20043. inhibition protects from multi-organ failure in a murine model of systemic inflammation. Blood. Nov 2007; 110(9): • Mridula Rewal, Rachel Jurd, T. Michael Gill, Dao-Yao He, 3176–3182. Dorit Ron, and Patricia H. Janak. α4–Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Antibody Intake of Alcohol. J. Neurosci. Jan 2009; 29(2): 543–549. • Wang Y, Tandan S, Cheng J, Yang C, Nguyen L, Sugianto J, Johnstone JL, Sun Y, and Hill JA. CaMKII-dependent remodeling of Ca2+ current in pressure-overload heart Tokyo, Japan Paris, France Protein failure. J. Biol. Chem. Sep 2008; 283(37): 25524–25532. • Cheng Du, Meena Jaggi, Chuanyou Zhang, and K.C. Balaji. Global Services, Local Access Global Services, Local Protein Kinase D1–Mediated Phosphorylation and • Pablo A. González, Carolina E. Prado, Eduardo D. Leiva, Subcellular Localization of β-Catenin. Cancer Res. Feb 2009; Leandro J. Carreño, Susan M. Bueno, Claudia A. Riedel, and 69(3): 1117–1124. Alexis M. Kalergis. Respiratory syncytial virus impairs T cell activation by preventing synapse assembly with dendritic • Mala M. Shah, Michele Migliore, Ignacio Valencia, Edward C. cells. PNAS. Sep 2008; 105(39): 14999–15004. Cooper, and David A. Brown. Functional significance of axonal Kv7 channels in hippocampal pyramidal neurons. GenScript at a Glance PNAS. Jun 2008; 105(22): 7869–7874.
• Global presence with local access • Animal facility with AAALAC accreditation G en • 24-hour customer support • Full client IP protection S
• ISO 9001:2008-certified and cGMP compliance cript U S A Inc.
GenScript USA Inc. GenScript USA Customer Testimonials
"I think GenScript provides absolutely excellent products in a timely "I was truly impressed and satisfied with the excellent service I got fashion. I have primarily used GenScript to order codon optimized from GenScript. All my queries were promptly handled in a artificial genes. Ordering artificial genes on their website or via Email professional manner. I made use of the custom antibody services, makes this especially facile because I can simply inquire about a gene, which included antigenic peptide design and production. During the get a quote and then get a quote. The whole process is fast and the course of the project, I was kept up to date with regular progress turnaround time for obtaining the finished product is usually less than reports. After completion of the project, GenScript contacted me to a month. I also think their technical support are wonderful people, confirm that I was satisfied with the product. In all the performed they have been most helpful in facilitating the process. I am so assays, the antibody proved to be highly specific. I would highly impressed with them that I have recommended them to several of my recommend GenScript services to anybody." colleagues." — Dr. Soné Ungerer, University of Pretoria, South Africa ― Dr. Maria Schumacher, The M.D. Anderson Cancer Center, University of Texas "GenScript Western kits have saved me weeks or months of research time! I absolutely love them! And the customer service is excellent, "GenScript is the company that I have entrusted my project with. The friendly and efficient." delivery of your gene synthesis is on time and the OptimumGeneTM ― Dr. Sarah Yohannan, Yale University School of Medicine technology on your Gene-on-Demand® gene synthesis platform has increased my gene expression dramatically. I am very happy with my results and GenScript is always my first choice for gene services." ― Bin He, Bristol-Myers Squibb, USA
- 3 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 4 - Bio-Reagent Center Bio-Reagent Center Molecular Biology Services Custom Antibody Services —One-Stop Solutions for Biology Research! Gene Synthesis 10 Custom Polyclonal Antibody Services 58 Mutagenesis Services 15 Custom Monoclonal Antibody Services 68 Mutant Libraries (Protein Engineering) 17 Monoclonal Antibody Production Service 70 PCR Cloning and Subcloning Services 19 Express Antibody Services 72 Custom Plasmid DNA Immunochemistry Services 74 Preparation Services 20 Large-scale Antibody Custom ORF Cloning Services 22 Production Services 76 Custom Vector Construction Services 23 Immunoassay Development Services 77 Knock-in and Knock-out Vector Construction Services 24 Custom Cell Line Services siRNA and miRNA Services 25 Stable Cell Line Services for Protein Large-scale DNA Sequencing Services 28 and Monoclonal Antibody Production 79 CloneArchTM Clone Storage Custom Stable Cell Line Services and Management Center 30 for Assay Development 81 Transient Gene Expression service 82 Custom Protein Services Process Development and Transfer 83 Bacterial Expression System 33 Catalog Stable Cell Lines 83 Yeast Expression System 36 Ready-to-UseTM Frozen Cells 85 Insect Cell Expression System 38 Mammalian Cell Expression System 40 Large-scale Protein Production Service 42 Protein Purification and Characterization 44
Custom Peptide Services
Standard Peptide Synthesis 46 Peptide Modifications 47 Custom Peptide Library Services 48 Cosmetic Peptide Services 51 Mirror-image Peptide Services 52 Custom Recombinant Peptides 53 cGMP Peptide Synthesis 54 Peptide Purity and Application 56
Email: [email protected] Tel: 1-732-885-9188 Bio-Reagent Service Center Molecular Biology Services (http://www.genscript.com/bio-reagent_center.html) (http://www.genscript.com/molecular_biology_service.html) Bio-Reagent Center
GenScript is a leading biology CRO in the world and the largest gene synthesis supplier in the USA. Our bio-reagent service platform GenScript provides a comprehensive repertoire of molecular • Vector-based siRNA and miRNA: GenScript offers encompasses all aspects of biology research ranging from gene, protein, peptide, and antibody to cell line, which are corner stone of our biology services, ranging from gene cloning and characterization cost-efficient vector-based RNAi services stand on their business. With seamless integration of world-class talent, state-of-the-art equipment, and deep understanding of the economy of scales, to advanced genetic engineering and mutant libraries. We have Tet-on and inducible expression vectors with our acclaimed GenScript is offering superior customized services and quality products in the most time-efficient and cost-effective manner. delivered over 100,000 synthetic genes and constructs with the 2nd generation design tools. most complex and difficult traits through our Gene-on- • Large-scale DNA Sequencing: With years of experience, DemandTM de novo synthesis platform. Our proprietary GenScript offers quick, high-quality, and cost-effective DNA CloneEZ® seamless cloning technology is capable of placing sequencing services. We perform all sequencing services in Target Identification Assay Development Lead Identification Pre-clinical any gene in any vector independent of restriction and ligation, a GLP-compliant laboratory environment, and the data our & Validation & Screening & Optimization Development seamlessly. Our CloneArchTM clone storage and management service generates meet the requirements of FDA submission. system provides our customers with worry-free clone storage We have processed hundreds of thousands of samples, and and a portal to all molecular biology, simply one-click away. have the capacity to sequence thousands of samples per day. Bio-Reagent Center Molecular Biology Services Cell Line Services • CloneArchTM Clone Storage and Management Center: • Gene synthesis • Stable cell lines Service List: CloneArchTM is a worry-free clone storage and management • Custom cloning • GPCRs/Ion channels system. It prevents unnecessary clone loss, preserving • Mutagenesis • Frozen cells TM • Vector construction • Gene Synthesis: GenScript's Gene-on-Demand service precious time that can be reinvested in more productive • Plasmid preparation Protein Services Antibody Services offers synthesis of any gene. Our advanced OptimumGeneTM biological research and drug development projects. Peptide Services • Bacterial expression • Custom mAb services codon optimization technology can optimize your gene • Yeast expression • Peptide synthesis • Custom pAb services sequence, allowing it to reach the highest possible level of • Insect cell expression • Chemical modifications • Express services gene expression and protein solubility. Our prices are 30% Competitive Advantages: • Mammalian cell expression • Peptide libraries • Western-GUARANTEED antibodies less expensive than those of our competitors. GenScript's • Soluble expression or refolding • Cosmetic peptides • Antibody purification TM • Large-scale production • Click Peptide Service special strategic partnership promotion campaign aims to • Advanced Gene Design Algorithms (OptimumGene ) • cGMP peptide synthesis establish long-term partnerships with customers who regard GenScript's proprietary and gene design technologies can GenScript's as preferred vender and provide them with significantly improve the expression level of target proteins. large-volume services at competitive price. In order to fit Our advanced optimization takes the entire mRNA structure your budget, GenScript will serve you in a flexible manner into consideration as opposed to traditional optimization Capacity, Capability, and Track Record under current economic conditions. methodologies which only consider the codon usage • Mutagenesis: Mutagenesis is one economical alternative to frequency and the structure of mRNA partially. The
de novo gene synthesis. GenScript offers cost-effective patent-pending OptimumGeneTM Gene Design Technology M w o e Experienced team—Seasoned scientific and management team mutagenesis as a complement to its gene synthesis service can typically increase at least 10-fold of protein expression lecular Biolo
rvi Track record—On time delivery for more than 90% of all orders package. level in E.coli, provided that the protein expression and e v Comprehensive package—Custom services & catalog products • PCR Cloning and Subcloning: GenScript provides PCR purification methods are appropriate. O cloning and subcloning services. This package is highly • CloneEZ® Seamless Cloning Technology
ces flexible and can be tailored to any specific set of project This new-generation, recombination-based cloning technology rvi
requirements. can accurately and efficiently assemble genes and gene gy ® Service
Se Gene: Antibody: Our recently developed CloneEZ seamless cloning fragments in any combination of sequences.
• Over 100,000 customized genes synthesized • ELISA titer at least 1:20,000 technology allows direct fusion of any DNA sequence in one • State-of-the-art Facilities • Over 1 million ORFs from 90 species • Positive Western guaranteed 30-minute procedure. GenScript is equipped with advanced bioscience facilities • Record length of > 50,000 bp gene synthesized • Multiple host species • Plasmid Preparation: Our services include industry-scale with a working area of about 20,000 square feet. s • Genes of complex sequences and difficult traits synthesized • Express service for fast turnaround plasmid production, batch tube aliquoting, labeling, • Comprehensive Package packaging, and step-by-step total QC management. We also offer mutagenesis, protein expression and Protein: Cell Line: GenScript currently offers SC Grade and Advanced SC Grade purification, and plasmid preparation. Our full complements • Over 2,000 batches of proteins delivered • Super stability for long-term production and scalability (Supercoiled content ≥ 95%, with enzyme-free and animal-free of upstream and downstream services provide our clients • Functional proteins fitting various downstream applications • Large-scale purification and characterization process) plasmids along with routine Research Grade with a one-stop source for all their laboratory needs. • Multiple expression systems • Wide variety of cell lines and recombinant proteins plasmid production to satisfy customers’ variable needs. • Experienced Team • Soluble expression or refolding rate > 90% • Downstream process development • Mutant Libraries (Protein Engineering): GenScript's We have synthesized more than 100,000 genes and • Analytical support and stability studies strong expertise in de novo gene synthesis allows us to constructs de novo. Our record length for gene synthesis is Peptide: synthesize complex protein libraries without any dramatic over 50,000 bp. We routinely synthesize long constructs increase in cost. Our mutant library services include (> 10 kb), complex genes, and genes with extremely high or • Peptide length up to 200 AA site-directed mutagenesis libraries, sequential permutation low GC content, repetitive sequences, and strong secondary • Extensive modification offering scanning libraries, and randomized and degenerated structures. • Capacity of 6,000 peptides/month libraries. • Scale ranging from milligrams to kilograms • KI/KO Vector Construction: Record-proven expertise and experience enables GenScript to efficiently and reliably deliver traditional (or conditional) KI/KO constructs and other types of custom vectors for genetic engineering. Typical turnaround time is less than four weeks.
- 7 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 8 - Custom Gene Synthesis Services
Applications: (http://www.genscript.com/gene_synthesis.html) Bio-Reagent Center
Backed up by versatile proprietary technologies, GenScript GenScript's proprietary technology guarantees the delivery of constructs for customers around the world. Over the years, molecular biology services are applicable in many kinds of custom de novo genes using only the gene’s sequence. Our GenScript has fine-tuned and empowered its technology procedures, such as cDNA production, heterogeneous protein mature technology allows us to concurrently produce double- platform, which combines advanced gene design tools expression, and the creation of gene variants and recombinant stranded synthetic genes over 50,000 bp and perform oligo (OptimumGeneTM) and tricky methods for the synthesis of antibodies. synthesis involving only single-stranded DNA of over 100 GC-rich and extremely long genes. It has allowed GenScript to bases. offer its customers with the most challenging genes. Early Drug Discovery Research • Target identification & validation As one of the pioneering companies in gene synthesis service • Assay development & screening industry, GenScript has synthesized over 100,000 genes and • Lead identification & optimization • Pre-clinical development
Bio-Reagent Center Bio-Reagent Biologics • DNA vaccines • Recombinant antibodies Custom Gene Synthesis • Therapeutic proteins
Clinical Research
• Biomarkers GenScript Verisign The Biology CRO • Genetic SNPs Secured
Order by email, Requested/optimized Transmission Custom Cloning QC by Delivery of phone, fax, or online sequence of sequence gene synthesis into vector sequencing gene product via server M s olecular Biolo
Competitive Advantages: Delivery Specifications: Service • Gene-on-Demand® gene synthesis platform gy Gene Standard delivery time (Business days) Proprietary technology to enable mutation-free synthesis of 0–1,000 bp 12 days* gy genes of any length and complexity 1,001–3,000 bp 17 days*
S
> 3,000 bp Upon request ervice • Optimized gene expression level and protein solubility Our proprietary OptimumGeneTM technology *Applicable to genes without complex sequences
comprehensively optimizes factors that are critical to s Molecular Biolo • 4 μg lyophilized plasmid containing your gene insert transcription, translation, and co-translational protein folding • Sequence chromatograms covering your gene (electronic) to achieve the highest possible levels of expression in any • Construct map for the plasmid (electronic) system. • Quality assurance certificate
• State-of-the-art facilities Pricing: GenScript is equipped with advanced bioscience facilities with a working area of about 20,000 square feet. • GenScript’s gene synthesis is the most competitively priced service in the market. The client retains all rights to • Any gene in any vector, seamlessly the sequence data and related intellectual property. At CloneEZ® seamless cloning technology, GenScript's new GenScript, precise and efficient gene synthesis at a better recombination-based cloning system, can efficiently fuse price is an attractive alternative to PCR cloning. your gene precisely into any site on any vector in less than 30 minutes. • GenScript's special strategic partnership promotion program offers a benefit-sharing opportunity with • Cost-effective customers who would regard GenScript as a preferred The competitive prices of our economy-of-scale services can vender or long-term partner. GenScript will provide help to ease your budget crunch. large-volume services at very competitive prices.
For more information, please call us at 1-732-885-9188, or email us at [email protected].
- 9 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 10 - OptimumGeneTM Codon Optimization
Gene-on-Demand® Platform: (http://www.genscript.com/codon_opt.html) Bio-Reagent Center
GenScript’s OptimumGeneTM gene design system is a proprietary Genes of any length and De Novo Gene Before Optimization gene optimization technology that aims to achieve the highest complexity Synthesis possible levels of productivity in any given expression system by
Gene up to 50,000 bp altering both naturally occurring and recombinant gene sequences. The OptimumGeneTM algorithm considers a variety of critical High GC content OptimumGeneTM CloneEZ® ® factors involved in different stages of protein expression, including Codon Optimization Gene-on-DemandGene-on-Demand Seamless Cloning Repeat sequence codon adaptability, mRNA structure, and various cis-elements in transcription and translation.
• Fruitful results in even the most difficult sequences: GenScript's painstakingly crafted algorithms can optimize Quotations and Ordering: even highly repeated sequences, extremely long sequences, Bio-Reagent Center Bio-Reagent Relative Position of Codons and sequences having large numbers of parameters. CAI: 0.7557 Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. • Comprehensive usage tables for optimization in any host: GenScript boasts an extensive collection of proprietary After OptimumGeneTM Optimization codon usage tables, allowing you to use any host, any time Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis in either basic research or industrial applications. Specifically, we have dependable codon tables for organisms, such as Email: [email protected] Pichia and Sf9, which are widely used in development procedures. Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 • DNA vaccine design: GenScript provides an effective Fax: 1-732-210-0262; 1-732-885-5878 solution to your vaccine needs by screening and optimizing parameters involved in regulating vaccinal gene expression M
s and immunogenicity.
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data olecular Biolo Relative Position of Codons • Customized solutions: The optimization conditions can be CAI: 0.9586 tailored to meet the specific needs of even the most esoteric Service project. Fig. 1.2: The distribution of codon usage frequency along the length of gy the gene sequence. A CAI of 1.0 is considered to be perfect in the desired expression organism, and a CAI of > 0.9 is regarded as very good, in terms of high gene expression level. gy
TM
OptimumGene Codon Optimization Success S Records: ervice
GenScript has accumulated years of experience in codon s Molecular Biolo Struggling with Cloning? optimization and gene synthesis. Our codon optimization ® system has optimized over 50,000 sequences in almost all Try GenScript Gene-on-Demand Services! major expression systems.
Other Insect Plant 2% 3% 6% E. coli Yeast 29% 18%
Mammalian 42%
Fig. 1.1: Based on our historical records, the OptimumGeneTM codon optimization has optimized over 50,000 sequences in almost all major expression systems.
- 11 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 12 - Large-scale Gene Synthesis Services
Case Study: (http://www.genscript.com/gene_synthesis_large_scale.html) Bio-Reagent Center
GenScript's proprietary OptimumGeneTM codon optimization and gene design technologies can significantly improve the expression Synthetic genes are playing an increasingly important role in • CloneEZ® seamless cloning technology is a new level of target proteins. Our advanced optimization takes the entire mRNA structure into consideration as opposed to traditional modern molecular and system biology. Utilizing advanced generation recombination-based cloning technology that can optimization methodologies which only consider the codon usage frequency and the structure of mRNA partially. The patent-pending technology platform, GenScript possesses one of the largest accurately and efficiently assemble genes and gene OptimumGeneTM Gene Design Technology can typically increase at least 10-fold of protein expression level in E. coli, provided that the gene synthesis capacities in the world and has the expertise fragments with any sequence combination. protein expression and purification methods are appropriate. and resources to commit to large projects. Our large-scale gene synthesis services of superior quality are among the most Experienced Team: Figures below manifested the effectiveness of the OptimumGeneTM technology in increasing protein expression levels for two different cost-effective services in the industry. genes expressed in E. coli. • We have synthesized more than 100,000 genes and Advanced Technologies: constructs de novo. Expression Results of Protein α • Our gene synthesis record is over 50,000 bp in length. • OptimumGeneTM codon optimization tool offers a • We routinely synthesize long constructs (> 10 kb), complex proprietary technology that comprehensively optimizes DNA genes, genes with extremely high or low GC content,
Bio-Reagent Center Optimized by Native Sequence KD M W/O Optimized by GenScript’s sequences for it to reach the highest possible expression in repetitive sequences, and strong secondary structures. Induction Competitor GA OptimumGeneTM W/O Optimization 150 M any system. 75
50 Protein α Protein Expression Level (mg/L) 32 W/O Optimized by Optimized by Native Sequence Quotations and Ordering: Induction Competitor GenScript’s W/O Optimization OptimumGeneTM Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Fig. A. GenScript's OptimumGeneTM codon optimization delivered 10 times higher expression level for protein α compared to non-optimized native gene the most convenient ordering method. sequence. The expression level was 3 times more than that from Competitor GA's optimization method.
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis M
s Expression Results of Protein β Email: [email protected] olecular Biolo
Optimized by Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 W/O Optimized by GenScript’s Native Sequence
Service Induction Competitor GA OptimumGeneTM W/O Optimization KD MM gy Fax: 1-732-210-0262; 1-732-885-5878 32
25 gy Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
S Protein Expression Level (mg/L)
Protein β ervice W/O Optimized by Optimized by Native Sequence 15 Induction Competitor GenScript’s W/O Optimization OptimumGeneTM s Molecular Biolo Fig. B. GenScript's OptimumGeneTM codon optimization delivered 20 times higher expression level for protein β compared to non-optimized native gene Related Services: sequence. The expression level was 13 times more than that from Competitor GA's optimization method. • Mutagenesis • Mutant Libraries (Protein Engineering) • PCR Cloning and Subcloning • Knock-in and Knock-out Vector Construction
Struggling with Cloning? Try Gene-on-Demand® Services!
- 13 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 14 - Mutagenesis Services
(http://www.genscript.com/mutagenesis.html) Pricing: Bio-Reagent Center
Mutagenesis is central to molecular biology, facilitating research GenScript's mutagenesis services, powered by sophisticated Cat. No. Promotion Price per mutation in a variety of fields, including studies of gene regulatory gene synthesis technology and high performance GS SC1441 Gene Synthesis and Mutagenesis Bundle Service $149a,b elements, DNA-protein interaction, protein structure/function polymerase, include point mutations, deletions, and insertions. SC1023 Standard Mutagenesis $169b relationship, enzyme active sites, and novel proteins. Note: a. Bundle discount is provided to orders with template gene synthesized at GenScript at the same time. b. The list price is valid for genes less than 1 kb in length. For genes longer than 1 kb, an additional $100 per kb will be applied. One mutation is defined as any combination of mutations within a 10-base frame.
Bio-Reagent Center Quotations and Ordering:
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form.
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis
Quotation Form: Obtainable on our website Competitive Advantages: Sample Submission Requirements: Email: [email protected] • Unparalleled Accuracy: GenScript's high performance GS Please provide the following information: polymerase ensures accuracy of mutants, avoiding creation • The complete sequence of the template including the target
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 M s
of unwanted mutations. gene and its vector olecular Biolo • Unlimited Sites: Using our sophisticated mutagenesis • Mutation specifications Fax: 1-732-210-0262; 1-732-885-5878 technology, GenScript can introduce mutations at any site. • Maps and antibiotic resistance of the template and destination
Service • Mutation on Large Construct: Our technology is optimized to vectors Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
gy introduce mutations, insertions, and deletions on constructs • Recommended to send 4 μg of template DNA as the starting up to 12 kb (including target gene and its vector). material. If the starting material is bacterial culture, please • Comprehensive Service Package: We offer comprehensive send the culture at sufficient amount. gy
upstream and downstream services, flowing from template S ervice sequencing and expression vector construction to protein expression and purification. s
Molecular Biolo Delivery Specifications:
Services: Our typical turnaround is about 10 business days. Our delivery package includes: Our mutagenesis service pipeline includes the following: • 4 μg of lyophilized destination vector containing the gene • Sequence Confirmation: The sequence of template DNA • Sequence chromatograms covering your gene (electronic) will be confirmed by sequencing upon request. • Construct map for the plasmid (electronic) • Primer Design/Synthesis: Primer design and synthesis for • Quality assurance certificate mutagenesis are included. • Mutation Introduction: Mutations are introduced by PCR. • Mutant Screening: Every mutation is verified by sequencing. • Mutant DNA Preparation: GenScript can manufacture mutant DNA plasmid up to kilogram scale with required purity and endotoxin levels.
- 15 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 16 - Mutant Libraries (Protein Engineering)
(http://www.genscript.com/synthetic_library.html) Quotations and Ordering: Bio-Reagent Center
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose In vitro molecular optimization is a very efficient means of the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, generating mutant proteins with improved or novel properties, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. identifying regulatory sequences, and probing for structurally and functionally critical residues. Synthetic libraries constructed using in vitro molecular optimization method are useful approaches toward systematic studies of protein’s properties, Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis regulations, and functions. GenScript's strong expertise in de novo gene synthesis allows Email: [email protected] us to synthesize complex protein libraries without any dramatic The final delivery of our sequential permutation library includes increase in cost. Our synthetic library services include the following: Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 site-directed mutagenesis libraries, sequential permutation • Up to 10 μg of mutation constructs scanning libraries, and randomized and degenerated libraries. • Mixed-mutagenesis library up to 20 variants for each Fax: 1-732-210-0262; 1-732-885-5878 Bio-Reagent Center position, delivered as one small library • Separated variants (optional) Secured Web Server: https://www.genscript.com/ssl-bin/secure_data Services: • Sequence verification information • Statistical analysis of nucleotide distribution at the mutation • Site-Directed Mutagenesis Libraries SC1153 sites GenScript combines its expertise in de novo gene synthesis and site-directed mutagenesis into an excellent site-directed • Randomized and Degenerated Libraries SC1155 Related Services: mutagenesis library construction service. The site-directed With our advanced degenerated oligonucleotide techniques, mutagenesis library offers a great platform for protein GenScript can generate any form of randomization or • Custom Vector Construction Services function and active center studies. In these libraries, any degeneration of the full-length gene in a synthetic DNA • Mutagenesis Services given residue can be substituted with any of other 19 fragment. This permits controlled, highly precise randomization • Knock-in and Knock-out Vector Construction Services common amino acids, creating systematic combinations of within oligonucleotides. • Protein Expression and Purification amino acid mutations that reveal any significant pattern.
GenScript's in vitro library synthesis technology can M s introduce random substitutions for maximum controlled olecular Biolo flexibility. The mutation frequency can be set to any value between 1 and 20 mutations per kb. A peer group of 48, 96, Service or 192 individual transformants is sequence-verified. gy gy
S ervice
The final delivery of our site-directed mutagenesis library
includes the following: s Molecular Biolo • Up to 10 μg of mutation constructs • Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library • Separated variants • Sequence verification information • Statistical analysis of nucleotide distribution at the mutation sites
• Scanning Point Mutation Libraries SC1154 Sequential permutation scanning is a systematic means of improving protein performance. It outperforms standard The randomized and degenerated libraries can be delivered in alanine/cysteine scanning by replacing each amino acid with any of two different formats: all 20 amino acids simultaneously. This technique provides • Degenerated PCR Products Library (up to 1011 variants): a detailed profile of each amino acid at the position. For 10 μg of linear DNA ready to clone via 5' and 3' restriction each codon of interest, a small, site-saturated library is sites. constructed. This library can be delivered as a pool or in a • Cloned Library (up to 109 transformants): Library subcloned separated format for any substitution variant (19 in total). into customer's vector. Glycerol stock, containing total The application of GenScript's expertise in de novo gene library transformants. synthesis to the field of sequential permutation scanning allows us to provide superb sequential permutation scanning library construction services.
- 17 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 18 - PCR Cloning and Subcloning Services Custom Plasmid DNA Preparation Services
(http://www.genscript.com/pcr_cloning.html) (http://www.genscript.com/custom_plasmid_preparation.html) Bio-Reagent Center
Are you tired of PCR cloning and subcloning? GenScript’s PCR Delivery Specifications: GenScript plasmid DNA preparation services cover individual Key Features: cloning and subcloning services can save you the time and constructs for research labs as well as large scale manufacturing energy doing these tedious and routine lab work, allowing you Our typical turnaround is around 14 business days depending for biotech suppliers and pharmaceutical companies. • Flexible scale: up to gram level. Starting from $50.00 for a to perform more focused and creative research. Our service is on the complexity of the project. Our delivery package includes GenScript’s high quality plasmids will help you achieve highly MidiPrep (100 μg) highly flexible and can be tailored to fit any specific project the following: efficient cell transfection, DNA vaccination, antibody production, • Completely enzyme-free and animal-free process for SC requirements. The starting materials are also flexible: we • 4 μg of lyophilized destination vector containing the gene and other preclinical studies. Grade and Advanced SC Grade plasmids' production accept PCR products, plasmid DNA, or bacterial culture. • Sequence chromatograms covering your gene (electronic) • Reproducible quality of plasmid DNA by archiving in Depending on the nature of the project, our typical turnaround is • Construct map for the plasmid (electronic) GenScript USA Inc. has upgraded its plasmid preparation CloneArchTM Clone Storage and Management Center 14 business days. • Quality assurance certificate platform. We are currently offering SC Grade and Advanced • Stringent and comprehensive quality control: at least seven SC Grade (Supercoiled content ≥ 95%, with enzyme-free and items, including appearance, A260/280, supercoiled content, animal-free process) plasmids along with routine Research residual RNA, genomic DNA, concentrations, restriction Sample Submission Requirements: Pricing: Grade plasmid production to satisfy customers’ variable needs. analysis, and endotoxin level (upon request) Bio-Reagent Center • Fast delivery time PCR products, plasmid DNA, and bacterial culture are all Cat. No. Price per PCR cloning or subcloning accepted. Please provide the following: SC1017 Starting at $295.00* • The complete sequence of the template including the target gene and its vector *The price listed above is only for genes shorter than 1 kb. For longer Services: • 2 μg of template DNA if the starting material is DNA. genes that require full sequencing, we add an additional $100.00 per kb for screening and sequencing. • A sufficient amount of bacterial culture if the starting material Quality Grades Features Typical Applications *The destination vector must be a commercially available vector. is bacterial culture Research Grade Predominantly Supercoiled • Typical molecular biology research • Maps, antibiotic resistance sites, and cloning sites of destination vectors • Recommended for effective transfection and co-transfection ≥ 95% Supercoiled • Antibody production SC Grade ≤ 0.03 EU/μg Endotoxin • Protein manufacturing
Quotations and Ordering: • Animal studies M olecular Biolo • Gene vaccine and gene therapy studies Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, • Highly effective transfection and co-transfection • Antibody production 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. ≥ 95% Supercoiled Advanced SC Grade • Protein manufacturing ≤ 0.005 EU/μg Endotoxin • Animal studies gy
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis • Gene vaccine and gene therapy studies S ervice
Quotation Form: Obtainable on our website s Email: [email protected] Additional Services: Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 • Bio-burden Assay SC1335 Fax: 1-732-210-0262; 1-732-885-5878 • Endotoxin Assay (LAL test) SC1336
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data Sample Submission Requirements:
• Plasmid DNA template of > 1 μg with a concentration of > 100 ng/μl. • Dissolved in water, TE buffer or dry in 1.5/0.5ml centrifuge tube. • Bacteria stock and plasmid DNA that is dry on paper are acceptable but not recommended.
- 19 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 20 - Custom ORF Cloning Services
Quotations and Ordering: (http://www.genscript.com/genpool.html) Bio-Reagent Center
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose The GenPoolTM ORF clones database contains over 1 million • DYKDDDDK-tagged cloning the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, open reading frames (ORFs) from 90 species, and the number ORFs cloned into pDream vector are tagged with a 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. is still growing! GenScript ORF cloning services, combined with DYKDDDDK tag. This facilitates the analysis of its our advanced Gene-on-Demand® gene synthesis technology, expression utilizing anti-DYKDDDDK antibody (GenScript, provide easy, quick, and sophisticated means to turn DNA A00187). This epitope tag is the most widely used epitope Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis sequences into expression-ready clones. tag employed in proteins’ structural and functional studies. The tag can be easily removed using enterokinase (EK, Email: [email protected] GenScript, Z01003). Competitive Advantages: Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 • GenPoolTM ORF clones database Delivery Specifications: Fax: 1-732-210-0262; 1-732-885-5878 Our ORF clones database includes over 1 million clones Bio-Reagent Center Bio-Reagent from 90 different species. Customer may search and Our typical turnaround time is one week. If the ORF clone of your Secured Web Server: https://www.genscript.com/ssl-bin/secure_data retrieve ORF information from the GenPoolTM ORF clone interest is not physically available, or it needs to be cloned into a database. vector other than pDream, the turnaround time is about 14 • Gene-on-Demand® gene synthesis technology platform business days. Our proprietary technology platform is a combination of • 10 μg lyophilized destination vector containing the gene of several advanced technologies and tools, including your interest GenPoolTM ORF clones database, OptimumGeneTM codon • Sequencing data of each clone (electronic version) optimization software, vector collection, and clone archive • Construct maps for the plasmid database. • Quality assurance certificate • Expression-ready cloning ORFs can be cloned into our pDream vector, which can be used directly in three different expression systems: bacteria, Pricing: Sf9 cells, and mammalian cells without any further subcloning.
Not only that, ORFs can still be cloned into other commercial M s Cat. No. Price per PCR cloning olecular Biolo or custom vectors. SC1200 Quote* *Please use our online quotation system for request. Service gy
Quotations and Ordering: gy
S
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose ervice the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript,
120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. s Molecular Biolo
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
- 21 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 22 - Custom Vector Construction Services Knock-in and Knock-out Vector Construction Services
(http://www.genscript.com/vector_construction_outsourcing.html) (http://www.genscript.com/targeting_vector_construction.html) Bio-Reagent Center
The construction of reliable targeting vectors is a prerequisite • Comprehensive targeting vector bank The ability to construct reliable targeting vectors is a prerequisite certain development stage. GenScript provides targeting for the generation of genetically engineered animals. Typical • Superior efficiency and fast turnaround in vector construction for generating genetically engineered animals. GenScript's vectors in specific tissues in a temporally specific manner. vector construction includes traditional and conditional and library screening advanced molecular biology platform has provided thousands of Our scientists have years of experience in modifying DNA knock-out, knock-in, and BAC construction . For many projects, targeting vectors to customers worldwide. These targeting sequences into customized targeting vectors, especially in targeting vector construction is a critical step in genetically GenScript USA Inc. has years of experience in knock-out and vectors have been successfully used in traditional and inserting loxP and FRT sequences. engineered animal research. However, without crucial skills knock-in vector construction. GenScript has provided hundreds conditional knock-out, knock-in, and many other genetic • Vector construction for knock-in SC1163 and experience for specific procedures required, targeting of targeting vectors to customers worldwide. These have been modification projects. Reporter gene knock-in provides a more predictable vector construction can become a time-consuming and costly used successfully in traditional and conditional knock-out, expression pattern than random integration (transgenic project. knock-in, and other genetic modification projects. With our methods). Similar to conventional knock-out service, services tailored to each customer’s exact requirements, typical Key Features: GenScript offers targeting vectors for efficient and precise turnaround time for targeting vector projects is usually less than genetic engineering. The knock-in service involves Competitive Advantages: four weeks. • Strong versatility: We can knock out or knock in any gene site-specific modifications in specific genes and reporter Bio-Reagent Center less than 20 kb on any site of any vector. gene knock-in. • Record-proven expertise and experience in genetic • Seamless cloning without undesired joint sequences: • Modification of targeting vector or BAC construction engineering GenScript’s proprietary CloneEZ® seamless cloning SC1164 • Gene synthesis capabilities as the largest gene synthesis technology can construct high-quality target vectors, GenScript has established several simple and effective supplier in the U.S. correctly and seamlessly. methods for plasmid and BAC modifications. This makes • Fast delivery and high success rate: Clients typically possible precise engineering, unlimited cloning and receive their custom knock-in and knock-out vector subcloning, and mutagenesis on DNA molecules of any size. constructs in less than four weeks. Our success rate has Quotations and Ordering: been kept over 95%. *GenScript's proprietary pGFk4.1 is available now! The vector is • Cost-effective solution: Most competitive prices designed for knockout vector construction containing a neo cassette flanked by two loxP sites. Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. Service Specifications: Delivery Specifications: M s olecular Biolo • Vector construction for traditional knock-out Our typical turnaround time for targeting vector projects is Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis (Conventional knock-out) SC1161 usually less than four weeks. Service delivery includes the Traditional knock-out involves deleting or disabling both
Service following: copies of a specific gene. This can be highly advantageous for
gy Email: [email protected] • 4 μg of targeting vectors both biomedical research and drug development. GenScript • Restriction enzyme digestion map for the vectors
has developed several powerful technology platforms gy Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 • Sequencing data covering the junction regions
combining de novo gene synthesis and quick cloning S techniques for efficient and precise generation of standard ervice Fax: 1-732-210-0262; 1-732-885-5878 Optional Features: and conventional knock-out targeting vectors. • 100 μg of targeting vectors
• Vector construction for conditional knock-out (Tissue- • Linear targeting vectors s Molecular Biolo Secured Web Server: https://www.genscript.com/ssl-bin/secure_data specific or developmental gene targeting) SC1162 • Targeting vectors with ultra-low endotoxin Conditional knock-out involves deleting or disabling a gene in only a particular organ, tissue, or cell type or only during a
Quotations and Ordering:
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form.
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
- 23 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 24 - siRNA and miRNA Services
(http://www.genscript.com/siRNA_interface.html) Quotations and Ordering: Bio-Reagent Center
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose GenScript RNAi technology has several advantages over GenScript's vector-based RNAi technology has been utilized to the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, synthetic siRNA and miRNA methods: it produces highly reliable produce a comprehensive collection of RNAi vectors, which are 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. siRNA and miRNA constructs at minimal cost, its products are frequently cited in our customers' scholarly and professional more stable and last longer, and it frees scientists from the publications. time-consuming and difficult task of RNAi vector construction. Secured Online Order: https://www.genscript.com/ssl-bin/order_sirna
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis
Email: [email protected] Vector-Based siRNA Services Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 Bio-Reagent Center (http://www.genscript.com/siRNA_service.html) Fax: 1-732-210-0262; 1-732-885-5878 Vector-based siRNA has stronger in vivo inhibitory effects than Service Description: synthetic siRNA and can work with tissue-specific promoters. Secured Web Server: https://www.genscript.com/ssl-bin/secure_data As regards the fact that stable cell lines can be developed, A small DNA insert (about 70–80 bp) encoding a short hairpin vector-based siRNA allows researchers to observe long term RNA targeting the gene of interest is cloned into a siRNA knock-down effects of their target genes. Not only that, the expression vector. The insert-containing vector can be used to inducible expression feature adds another layer of flexibility. transfect the cells, where it expresses the short hairpin RNA. As a powerful tool for gene function analysis and drug target validation, GenScript vector-based siRNA technology is an adaptation of GenScript gene synthesis technology. With our Delivery Specifications: OptimumRNAiTM technology and siRNA design tools, we can help you identify the most effective siRNA sequences with • Typical delivery: Includes a construct map, approximately M
s strong gene silencing effect, and clone them into any cloning 4 μg of lyophilized plasmid DNA containing the siRNA olecular Biolo vectors you desire. construct, and sequence data documenting each clone’s DNA sequence.
Service • Quantity: 4 μg (MiniPrep) or 100 μg (MaxiPrep*), and
gy Key Features: multi-milligram quantities are available. *For MaxiPrep orders, we offer our endotoxin elimination service. • Acclaimed siRNA Design Package: GenScript's 2nd • Quality Control: The insert is sequence-verified, and trace gy
generation siRNA design tool combines the industry's data is provided together with the plasmid. S ervice most advanced algorithms with GenScript's time-tested • Turnaround Time: About two weeks expertise. • Storage: Store at -20°C after delivery. s
Molecular Biolo 1. siRNA Target Finder: Identifies unique candidate siRNA target sequences in cDNA 2. siRNA Construct Builder: Builds small hairpin inserts from Pricing: siRNA targets for expression vectors 3. siRNA Sequence Scrambler: Creates negative controls for Cat. No. Description Price siRNA experiments SC1007 siRNA construction Quote 4. Genome-Wide Design: Provides a wide selection of human and mouse genes
From Design to Delivery, Choose Superior siRNA.
- 25 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 26 - Vector-Based miRNA Services Large-scale DNA Sequencing Services
(http://www.genscript.com/miRNA_services.html) (http://www.genscript.com/sequencing.html) Bio-Reagent Center
MicroRNAs (miRNAs) are small (~22 nucleotides), noncoding Key Features: DNA sequencing is an important component of genetic analysis, Large-scale Sequencing: RNAs expressed by metazoans and viruses. They are believed not only for fundamental biological process research but also to play a crucial role in eukaryotic development by controlling • Comprehensive miRNA vector collection for applied researches involving diagnostics, forensics, and GenScript has many years of experience in large-scale post-transcriptional gene expression. Equipped with years of • Custom cost-effective regenerated vectors gene therapy. sequencing. Our large-scale sequencing service is de novo, experience and expertise, GenScript has developed an • Strong silencing effects which does not require any pre-existing information about the advanced vector-based miRNA service, which can help to ease • Fast, reliable results at a competitive price Armed with years of experience, GenScript offers efficient, sequence. Our large-scale DNA sequencing service package the difficulty involved in miRNA study. The resulting miRNA- cost-effective, and high quality DNA sequencing services. We includes the following: expressing plasmid is purified and ready to transfect. They can perform all sequencing activities in GLP-compliant laboratory be used in a variety of applications to down-regulate gene environment. GenScript has processed hundreds of thousands • High-throughput single- and double-stranded sequencing expression. of samples and our daily sequencing capacity reaches to • High-throughput DNA extraction/purification (96- or 384-well thousands. format) • Data delivery via our secure web server Bio-Reagent Center • Library sequencing (BAC, PAC, Cosmid, EST) Services: Key Features: • DNA sequence finishing and assembly
GenScript's vector-based miRNA technology offers you an efficient way to clone your sequences of interest into any desired vector. • Guaranteed accuracy and coverage: Our double strand The final delivery package includes the original electropherograms, sequencing technology guarantees the sequencing accuracy consensus sequence, and QC reports. Service Type Service Description Delivery Details and coverage of over 99.0%. • Long read through length: 700–750 bp for individual Q20 1. Our optimized SV40-CMV promoter causes 1. 4 μg of lyophilized plasmid DNA containing read length values increased expression of pre-miRNA Vector based pri-miRNA construction • High single pass rate: Our GLP-compliant laboratory the desired miRNA construct 2. Selection of cGFP fluorescent tags operation, sophisticated information management system 2. Detailed sequencing data documenting the 3. Final product is confirmed by stringent sequencing and strict quality control ensure a single pass rate as high as DNA sequence of each clone 1. Selection of cGFP fluorescent tags 92–98%. Vector-based pre-miRNA construction 3. The typical delivery time is about two weeks. 2. Final product is confirmed by stringent sequencing • Data integrity: Through encrypted email and secured website, GenScript has established its own information M s safeguarding guidelines to protect customers' sequence. olecular Biolo
Service Pricing: Primer Walking: gy
Cat. No. Service Price per Construct Primer walk sequencing is the method of choice for sequencing gy
SC1080 miRNA construction $500.00 DNA fragments whose lengths are between 1.3 and 7 kb. It is S applicable to PCR amplifications, plasmid inserts, or even entire ervice Note: Maxiprep plasmid should be used for transfection. You may order MaxiPrep (> 100 μg) from GenScript starting at $50.00. plasmids. GenScript's primer walk sequencing service is
divided into single-strand primer walks and double-strand s Molecular Biolo primer walks according to the customers' specifications. Our service package includes the following: Quotations and Ordering:
• Primer design and synthesis Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose • Sequencing the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, • Primer design and synthesis 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. • Sequencing data delivery/retrieval via secure web server • Sequencing reactions • Dye terminator removal Secured Online Order: https://www.genscript.com/ssl-bin/order_miRNA • DNA sequence finishing and assembly
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
- 27 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 28 - CloneArchTM Clone Storage and Management Center
Sample Submission Requirements: (http://www.genscript.com/clonearch.html) Bio-Reagent Center
Template Concentration Volume/Reaction Solvent CloneArchTM is a worry-free clone storage and management Advantages in clone management: Genomic DNA 50–100 ng/μl ≥ 5 μl ddH O or 10 mM Tris 2 system. It prevents unnecessary clone loss, preserving
PCR fragments 20 ng DNA/100 bp ≥ 8 μl ddH2O or 10 mM Tris precious time that can be reinvested in more productive • One-click away for further construct manipulation
Plasmid DNA 200–500 ng/reaction ≥ 8 μl ddH2O or 10 mM Tris biological research and drug development projects. • Comprehensive record-keeping of all cloning information As the world's leading provider of molecular biology services, with easy online tracking and 24/7 accessibility Note: • Our large-scale sequencing service currently accepts orders involving 192 samples or more. GenScript has synthesized, engineered, and stored hundreds of • Save all the hassles involved in clone search • For combined submissions of templates and primers, the templates must meet our sample submission requirements, and the primers must be submitted thousands of our customer's clones. Our commitment to your • Save material transfer time and cost in quantities of at least 20 μl each (10 pmol/μl or 10 ng/μl). success ensures that your construct and clone information are • Standard primers are available at no extra charge [M13 (-21), M13 rev, SP6, T7, T3, BGH, GL2, RV3, NCMV30, CCMV24]. stored with all due confidentiality. With minimal cost, CloneArchTM can bring you the peace-of-mind on your clones. Bio-Reagent Center Pricing: Advantages in clone storage:
Cat. No. Service Price • GLP-compliant storage at two separate geographic SC1201 Genomic DNA Sequencing $7.00/reaction locations SC1202 PCR Fragments and Plasmid DNA Sequencing $5.00/reaction • Barcode on each individual clone with absolute precision SC1203 Plasmid DNA Extraction from E. coli (E. coli Recovery, Plasmid DNA Extraction) Additional $3.00/reaction • Guaranteed security and confidentiality
Quotations and Ordering: Quotations and Ordering:
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Call 1-732-885-9188 or email [email protected] for more information. the most convenient ordering method. For sample submission if applicable, please send to Molecular Biology Services, GenScript, For limited time, we are offering free clone storage.
120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. M
s Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. olecular Biolo
Secured Online Quotation: https://www.genscript.com/ssl-bin/quote_gene_synthesis Service gy Quotation Form: Obtainable on our website gy
Email: [email protected] S ervice
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 s Molecular Biolo Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
Give a boost to your research, order GenScript sequencing services!
- 29 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 30 - Custom Protein Services (http://www.genscript.com/protein_service.html) Bio-Reagent Center Protein Expression & Purification GenScript takes your project directly from gene synthesis to codon optimization technology can optimize your gene protein production! Our free rare codon analysis tool can sequence for highest possible level of gene expression and determine whether your gene sequence is optimal, and at the protein solubility. In addition, OptimumGeneTM algorithm Verisign protein GenScript same time explore means to boost your target protein optimizes a variety of parameters that are critical to Secured expression. transcription, translation, and protein folding. • Extensive recombinant protein production experience: Order by Transmission of Cloning Transformation Expression Protein QC by Delivery of Service List: We have delivered thousands of difficult proteins including email, sequence via into of purification multiple protein trans-membrane proteins, secreted proteins, proteases, and phone, fax, server expression target methods product or online vector protein • Bacterial Protein Expression：GenScript has developed other enzymes at a success rate greater than 90%. BacPowerTM technologies to tackle hard-to-express and • BacPowerTM technology and FoldArtTM protein hard-to-dissolve proteins. Guaranteed delivery of 3 mg refolding technology: Hard-to-express and hard-to- purified soluble protein, starting at only $2,200!
Bio-Reagent Center dissolve proteins are specifically tackled by our proprietary • Yeast Protein Expression：GenScript's proprietary BacPowerTM technology and FoldArtTM protein refolding YeastHIGHTM technology combines all the advantages of technology. bacterial and eukaryotic expression systems. Our yeast • Comprehensive services: GenScript provides a directional Case Study—Protein with Biological Activity expression system employs stable and durable production streamlined service platform that delivers projects directly strains that resemble mammalian protein processing. from gene synthesis, to protein expression, and further to (http://www.genscript.com/protein_case_studies.html) • Baculovirus/Insect Cell Protein Expression：GenScript stable cell line generation, antibody production, and assay has pioneered secretion methods for recombinant proteins development. Production and purification of bioactive proteins is essential in development teaming up to deliver enzyme with qualified from baculovirus-infected insect cells. With a capacity of • High quality services: GenScript provides high quality drug discovery. Utilizing our state-of-the-art equipment and specific activity, higher than the customer expected. After producing proteins in large quantities up to 100 liters per protein expression services to meet your stringent methodologies, multifunctional units at GenScript work together modifying the method of cell-breaking, we increase the final project, GenScript services suit to not only academic requirements. We strive to produce high purity, high activity to ensure successful delivery of bioactive proteins. A case yield of protein of interest with purity of above 90%. institutions but also pharmaceutical and biotechnology proteins in a timely fashion. The purity level of our study below shows groups of protein production and assay industries. deliverables has been over 95% with endotoxin level lower • Mammalian Cell Protein Expression：GenScript’s than 0.01 EU/μg. AB proprietary 293 and CHO suspension system platform 4000 KD Cus promotes fast production and delivery of recombinant Applications: 220 150
proteins and monoclonal antibodies up to grams level. 100 t o
75 m Pr • Large-scale Protein Production Service：On top of GenScript has years of experience in protein expression and Target 3000 Protein large-scale purification service, our versatile and reliable purification. Combining our expert specialists with industry- 50
leading technology and state-of-the-art facilities, we are ready 32 o
protein expression system, which includes fermentors, t
25 e
2000 in wave mixers, and bioreactors, allows GenScript to provide to deliver projects involving any expression system, construct, 15 Se proteins in gram levels to its clients in both public and or purification procedure for your individual needs. Aclivity (UL)
private research sectors, academia, and all levels of the • Target validation rvi 1000 • Enzyme characterization ces pharmaceutical industry. Fig. A. SDS-PAGE analysis of the fractions upon the IEX chromatography • High-throughput screening • Protein Purification and Characterization：GenScript Lane 1–47: Gradient elution fractions • Antibody production offers several methods for protein purification and Lane M: Low weight marker (GenScript, Cat. No. MM0906) characterization. We deliver proteins in different purity • Structural biology studies Lane S: The supernatant after homogenizer with high pressure levels, allowing customers to choose the ideal level of • Eukaryotic protein analysis Lane ft: Flow through purity for their intended downstream applications. • NMR studies with isotope-labeled proteins Fig. B. Enzyme assay monitoring the fractionation by • SAR (Structure-activity relationships) DEAD Sepharose Competitive Advantages: • Animal feed production • Environmental engineering CD • OptimumGeneTM codon optimization technology for TM gene expression: GenScript's advanced OptimumGene kDa M1 2345sftFig. C. Fractions pooled kDa MS Fig. D. SDS-PAGE analysis of the 220 150 based on activity analysis for 100 purified protein 100 75 Target 75 further ADP Sepharose affinity Target Lane M: Low weight marker Protein Protein 50 50 purification (GenScript, Cat. No. MM0906) 32 Lane M: Low weight marker 32 Lane S: The supernatant after 25 25 (GenScript, Cat. No. homogenizer with high pressure
MM0906) 15 15 Lane S: The supernatant after homogenizer with high pressure Lane ft: Flow through
- 31 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 32 - Bacterial Expression System
(http://www.genscript.com/custom_protein_bacterial_expression.html) Milestones Specifications Deliverables Timeline* Bio-Reagent Center Subcloning into one expression construct, 1. Subcloning Construct(s) report 2 weeks Bacterial protein expression systems are among the most 1. Guaranteed Package: under the choice of the client common and economical means of protein expression available Follow client's protocol requirements on TM today. However, two major problems often prevent researchers Using BacPower , GenScript can offer our customers a temperature, time course, and inducer 2. Pilot expression and from taking full advantages of the systems' advantages: guaranteed package for protein expression and purification. The concentration Expression data 1 week purification 1) The protein of interest cannot always be expressed using the package ensures the delivery of 3 mg or more of purified Appropriate E. coli strain specific vector/strain combination. soluble protein. SDS-PAGE gel and/or Western blot 2) The proteins expressed in bacterial system are sometimes 3. Protein expression One strain from above Purified protein insoluble. GenScript has developed BacPowerTM technologies E. coli Protein Expression—Guaranteed Protein Expression 1–2 weeks to tackle hard-to-express and hard-to-dissolve proteins. Package, 3 mg purified soluble protein starting at $2,200 and purification Follow client's purification protocol QC data SDS-PAGE, Western blot BacPowerTM technology package includes systematic (Cat. No. SC1253) procedures for expression optimization, such as testings of *Listed is the typical turnaround time of GenScript’s economic protein expression package. Please note that additional requirements may incur additional
Bio-Reagent Center expression vectors and bacterial strains, induction optimization, steps and extend processing time. and refolding. This service can be expanded to cover tag or endotoxin removal as well as additional custom purification for a separate fee.
Milestones Specifications Deliverables Timeline* Pricing • Subclone the gene into 3 expression vectors (Customer provides the gene or Cat. No. Service Price 1. Subcloning Construct(s) report 3 weeks GenScript synthesizes the gene for an SC1256 E .coli Protein Expression—Economic Protein Expression Package Starting at $1,750.00 additional fee)
• Transformation of 3 constructs into bacteria 3. Protein Refolding Service: • Expression optimization: induction duration, temperature, and IPTG 2. Expression optimization QC data 1–2 weeks and verification concentration Insoluble aggregates, also called inclusion bodies, are one of 3. Small molecular assistants:
• Coomassie blue stained SDS-PAGE gel the major issues facing protein expression in E. coli. The a. Denaturant Cus s • Yield estimation and feasibility proposal insoluble aggregates are non-functional but they completely b. Amino acids t block the research process toward downstream applications. o
for large-scale expression (optional) c. Polymers: micellar and liposomal m Pr ervice GenScript's protein refolding services provide a solution to this d. Reducing/oxidizing reagents S 3. Expression and • Expression in 1 L bacterial culture 3 mg or more of purified 1–2 weeks
problem. e. Salts: Na/K/Mg-Cl and (NH4)2SO4 o
purification • One-step affinity purification soluble tagged protein t f. Saccharides: glucose, sucrose, and trehalose e QC data in Our confidence is backed up by our proprietary FoldArtTM 4. Artificial chaperones: detergent and non-detergent Se protein refolding technology, which includes high-hydrostatic- 5. Matrix-assisted methods: SEC (size exclusion *Listed is the typical turnaround time of guaranteed protein expression package at GenScript. Please note that additional requirements may incur rvi
tom Protein pressure techniques, small-molecule additives, and column- chromatography), HIC (hydrophobic interaction additional steps and longer processing time. ces s
u refolding methods. chromatography), affinity column and IEC (ion exchange C chromatography) Pricing Services: 6. Chaperon-mediated methods: ATP-dependent and Upon request, protein refolding services can be incorporated to ATP-independent Cat. No. Service Price our Bacterial Protein Service Packages. Over 95% of the 7. Gradient methods: pH, Tem, hydrostatic pressure, and SC1253 E. coli Protein Expression—Guaranteed Protein Expression Package Starting at $2,200.00 inclusion bodies can be solubilized and refolded. We provide denaturant refolded protein at 85% purity or greater. The final output is dependent on the total amount of target protein comprising the Delivery Specifications: aggregates. The refolded protein is delivered in customized GenScript can provide soluble refolded protein at 85% purity or 2. Economic Package: buffer. Some major elements of GenScript’s protein refolding greater from one-liter expression system, based on total services are listed below. amount of target protein comprising the aggregates. The • Evaluation of target proteins' biochemical and biophysical refolded protein is delivered in customized buffer, along with the Our economic package targets proteins that can be easily Package starting at $1,750 (Cat. No. SC1256) properties protein’s purity and identity data determined from coomassie- expressed and those for which you can provide a standard • Refolding optimizations in the 1 L system stained gels and Western blot analyses using tag or the protocol. If customers could supply expression protocol for their proteins, • Selection of particular refolding strategy based on protein's customer's designated protein-specific antibody (must be we recommend our economic package using bacterial sequence and structural properties. Typically, our strategies provided by the customer). E. coli Protein Expression—Economic Protein Expression expression as a more economical solution. cover: 1. Direct dilution 2. Dialysis
- 33 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 34 - Yeast Expression System
4. Bacterial Fermentation Service (Cat. No. SC1027): (http://www.genscript.com/custom_protein_yeast_expression.html) Bio-Reagent Center
Large quantities of highly pure protein are a prerequisite for Services: GenScript's proprietary YeastHIGHTM technology combines all Grown in chemically defined media, the resulting purified research involving high throughput screening, functional GenScript's bacterial fermentation services are highly the advantages of bacterial and eukaryotic expression systems. secretory or intracellular proteins can be used for the following analysis, and structural biology. Large-scale protein production customizable. Expression and growth condition can be Our yeast expression system employs stable and durable applications: and purification often become bottlenecks for both core facilities optimized for large-scale production, purification, and production strains that resemble mammalian protein • Eukaryotic protein analysis and individual scientists’ work flow. characterization. Our bacterial fermentation capacity is as high processing. This system features high productivity and offers • NMR studies with isotope-labeled proteins as 1,000 L. We can produce transformed bacterial cell paste up high-yield protein expression, dramatically reducing the needed • Assay screening GenScript offers industry-scale bacterial fermentation service as to three metric tons and over 98% pure homogeneous cost and time. • Structural biology studies a solution to this bottleneck. Our service is highly customizable recombinant proteins up to grams. • Animal feed production to any specific project requirement. The capacity of our GenScript has developed a platform that allows humanized • Environmental engineering bacterial fermentation service is as high as 1,000 L. Sample Requirements: antibodies to be produced in yeast expression system. The GenScript prefers transformed bacterial culture as starting similarity between the yeast and mammalian system for Competitive Advantages: 1. YeastHIGHTM Advanced Package: Bio-Reagent Center materials for our fermentation service. Please provide detailed recombinant antibody expression enables GenScript to reduce • High capacities: GenScript's bacterial fermentation service instructions on fermentation conditions using our service order the high cost associated with manufacturing recombinant is available in scales ranging between 10 and 1,000 L. form. If starting material is an expression vector DNA, or if antibody in mammalian expression system. GenScript’s engineering-enhanced protein expression • Large-scale purification: GenScript provides fermentation condition optimization is required, additional fee technologies enable high protein expression yield and achieve comprehensive large-scale purification services that utilize applies. In addition, as the largest gene synthesis supplier in the U.S., a success rate of over 90% for a wide variety of recombinant affinity column, gel filtration, ion exchange, and GenScript can provide you with free advanced codon proteins. Our proprietary YeastHIGHTM technology pushes the hydrophobic-interaction column. Delivery Specifications: optimization specific to Pichia pastoris, Saccharomyces yield even higher. Many secretory proteins for which bacterial • Comprehensive package: GenScript offers a full range of • Cell pastes cerevisiae, and other yeast strains, to further enhance the expression systems failed to produce soluble or inactive upstream and downstream services, from gene synthesis • Batch records productivity of your protein. Our free rare codon analysis tool inclusion bodies can be produced successfully by our yeast and expression vector construction to protein purification • QC datasheets can determine whether your gene sequence is optimal, and at expression system using native or yeast secretion signals. and characterization. the same time explore means to boost your target protein • Competitive prices: Our bacterial fermentation service expression. offers the most competitive prices in the industry.
Milestones Specifications Deliverables Timeline* Cus
s • Subcloning into two appropriate Pricing expression vectors t 1. Subcloning Construct(s) report 2 weeks o
• Plasmid maxi preparation m Pr
ervice Cat. No. Service Price • Construct linearization S SC1027 Bacterial Fermentation Service Quote o
• Transformation of the linear construct into t e the appropriate host strain in • Confirmation of positive transformants via Se Quotations and Ordering: PCR rvi tom Protein ces s • Expression optimization and confirmation
u 2. Expression optimization Expression data 3–5 weeks
C 1. Screening of transformants with Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose and evaluation the most convenient ordering method. For sample submission if applicable, please send to Protein Services, GenScript, 120 multiple gene-insert with in-house Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. YeastFast technology 2. Media formulation and inducer concentrations 3. SDS-PAGE gel and/or Western blot Quotation Form: Obtainable on our website • Small-scale expression from 1 L Email: 3. Protein expression • One-step purification, or additional Purified soluble protein [email protected] 2 weeks and purification purification reaching a purity level of 90% QC data Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 or higher • Large-scale production up to 500 L Fax: 1-732-210-0262; 1-732-885-5878 4. Large-scale protein (Employs fermentors and TFF large-scale Yeast paste/ filtration system) production and purification Purified proteins 2–3 weeks Secured Web Server: https://www.genscript.com/ssl-bin/secure_data (Optional) • Deliverables available at a purity level of QC data 90% or higher • Tag removal
Guaranteed delivery of 3 mg purified soluble protein, *Listed is the typical turnaround time of GenScript’s YeastHIGHTM Advanced Protein Expression Package. Please note that additional requirements may incur additional steps and extend processing time. starting at only $2,200! (E. coli)
- 35 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 36 - Insect Cell Expression System
Pricing (http://www.genscript.com/custom_protein_baculovirus_insect_expression.html) Bio-Reagent Center
Cat. No. Service Price (USD) The Baculovirus Expression Vector System (BEVS) is expression and production. SC1346 Yeast Protein Expression—YeastHIGHTM Advanced Protein Expression Package Quote universally recognized as a powerful and versatile means of producing large quantities of recombinant proteins. Its ease of In addition, as the largest gene synthesis supplier in the U.S., use, high expression level, and ability to incorporate GenScript can provide you with free advanced codon 2. Standard Package: post-translational modifications make it a valuable part of any optimization specific to Sf9, Sf21, Hi-5, and other insect cell lines protein expression arsenal. GenScript’s baculovirus/insect cell to further enhance the your protein’s productivity. Our free rare expression service package includes virus and flexible-scale codon analysis tool can determine whether your gene sequence GenScript also provides a standard package for yeast expression service, which can be conducted in accordance with customized protein productions. GenScript has pioneered secretion is optimal and at the same time explore means to boost your protocol that customers may see fit. methods for recombinant proteins from baculovirus-infected target protein expression. insect cells. With a capacity of producing proteins in large Milestones Specifications Deliverables Timeline* TM quantities up to 100 liters per project, GenScript services suit to 1. BacuVance Optimization Package: • Subcloning into one appropriate
Bio-Reagent Center not only academic institutions but also pharmaceutical and 1. Subcloning expression vectors Construct(s) report 2 weeks biotechnology industries. For customers who wish to establish and scale up the protocols • Plasmid maxi preparation that best fit their projects, GenScript recommends them to use • Construct linearization GenScript protein expression specialists will work closely with BacuVanceTM optimization package to explore different • Transformation of the linear construct into you to clone and express full-length recombinant proteins from expression conditions for their target proteins. the appropriate host strain full-length cDNAs, EST sequence information, and genomic 2. Expression and • Confirmation of positive transformants via PCR Expression data 2 weeks sequences and offer expert advices at all stages of protein evaluation • Expression confirmation • SDS-PAGE gel and/or Western blot Milestones Specifications Deliverables Timeline • One liter yeast culture expression * 3. Pilot expression Purified proteins • One-step purification, or additional 2 weeks 1. Subcloning • Subcloning into three expression vectors Construct(s) report 2 weeks and purification QC data purification reaching a purity level of 90% or higher • Generation of recombinant Bacmid DNA • Large-scale production up to 500 L (Using • Transfection of insect cell with 4. Large-scale protein fermentors and TFF large-scale filtration system) Yeast paste/ 2. Virus generation recombinant Bacmid DNA 5 ml at > 107 pfu/ml viral stock 3–4 weeks
2–3 weeks Cus s production and purification • Delivery of proteins at > 90% purity or purified proteins • Generation of P1 stock (Low titer), P2 (optional) QC data stock (High titer), P3 determine pfu higher t o
• Tag removal m Pr ervice • Infection of insect cells with P3 stock
S 3. Expression optimization • Expression optimization: MOI, time *Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps and extend processing time. and pilot protein Expression data o 1.5 weeks t
course, cell lines e
productionpurification in • SDS-PAGE gel and Western blot Se
Pricing 4. Expression and • One liter insect cell expression Purified proteins rvi 1–2 weeks tom Protein
purification • One-step purification QC data ces s
u Cat. No. Service Price (USD) C SC1257 Yeast Protein Expression—Standard Protein Expression Package Quote *Listed is the typical turnaround time for GenScript’s BacuVanceTM Optimization Protein Expression Package. Please note that additional requirements may incur additional steps and extend processing time.
Quotations and Ordering: Pricing
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Cat. No. Service Price (USD) the most convenient ordering method. For sample submission if applicable, please send to Protein Services, GenScript, 120 SC1347 Baculovirus/Insect Cell Protein Expression—BacuVanceTM Quote Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. Optimization Protein Expression Package
Quotation Form: Obtainable on our website 2. Standard Package: Email: [email protected] If customers have already established protocols, GenScript’s choice. All of the following steps are executed in accordance Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 standard baculovirus protein expression package is the best with the customer's requirements.
Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
- 37 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 38 - Milestones Specifications Deliverables Timeline* Pricing Bio-Reagent Center 1. Subcloning • Subcloning into one expression vector Construct(s) report 2 weeks Cat. No. Service Price • Generation of recombinant Bacmid DNA SC1261 Baculovirus Preparation Service Quote 2. Recombinant virus • Transfection of insect cell with 7 generation recombinant Bacmid DNA 5 ml at > 10 pfu/ml viral stock 2–3 weeks • Generation of P1 stock (Low titer), P2 stock (High titer), and titer determination Quotations and Ordering:
3. Protein expression • Infection of insect cells with P2 stock Expression data 1.5 weeks evaluation • SDS-PAGE gel and Western blot Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For sample submission if applicable, please send to Protein Services, GenScript, 120 4. Expression and • One liter insect cell expression Purified proteins 1–2 weeks Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. purification • One-step purification QC data Bio-Reagent Center *Listed is the typical turnaround time for GenScript’s standard baculovirus protein expression package. Please note that additional requirements may incur Quotation Form: Obtainable on our website additional steps and extend processing time. Email: [email protected]
Pricing Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Cat. No. Service Price Fax: 1-732-210-0262; 1-732-885-5878 SC1258 Baculovirus/Insect Cell Protein Expression—Standard Protein Expression Package Quote Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
1. Baculovirus Preparation:
GenScript's recombinant baculovirus preparation services highly infectious, ready-to-use virus stocks, and detailed Cus
s guarantees consistent batch-to-batch virus. We provide titered, storage protocols. t Mammalian Cell Expression System o Milestones Specifications Deliverables Timeline m Pr ervice
S (http://www.genscript.com/bioprocess.html) • Subcloning into one expression vector Construct(s) report
1. Subcloning 2 weeks o t e
• Generation of recombinant Bacmid DNA in Transient Gene Expression (TGE) system offers a rapid and Competitive Advantages: • Transfection of insect cell with Se reliable solution to your protein production. One prominent
recombinant Bacmid DNA rvi 2. Virus generation Viral stock 2–3 weeks characteristic of the TGE platform is its ability to generate a •
tom Protein The proprietary 293 and CHO suspension system promotes ces
s • Generation of P1 stock (Low titer), P2 large quantity of protein samples. Expression levels of proteins u fast production and delivery of recombinant proteins and stock (High titer), titer determination C delivered via these suspension-adapted cells can reach 50–100 monoclonal antibodies up to grams level for your drug • Protein expression verification mg/L even under un-optimized conditions. Using the TGE discovery and bioanalytical assays. system, milligrams or even grams of recombinant monoclonal • The serum free cell culture condition makes the downstream antibodies can be generated within three to five weeks. Promoter purification processing much easier. GOI Tn7R Tn7L Helper Helper GenScript's transient mammalian expression system has been Transformation Donor Transformation used to produce a diverse range of protein products. The TGE Recombinant plasmid Bacmid donor plasmid Antibiotic selection system can easily produce proteins with flexible scales from several milliliters to 100 liters. We typically achieve high Competent DH 10Bac cells E. coli containing recombinant bacmid concentrations of harvestable and active products. The impressive success rate resulted from our transient production Extraction of platform has proven to be a reliable tool for rapid productions of recombinant bacmid DNA recombinant proteins and monoclonal antibodies. Recombinant virus particles
Determine viral titer via plaque assay Transfection of insect cells with recombinant bacmid DNA
Infection of insect cells Expression verification & Virus amplification Virus stock Recombinant bacmid DNA
- 39 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 40 - Large-scale Protein Production Service
Mammalian Cell Expression System: (http://www.genscript.com/custom_protein_large_scale_expression_purification.html) Bio-Reagent Center
Services Description Deliverables With the rapid growth of the protein drug market and recent expression system, which includes fermentors, wave mixers, • Amplification/isolation of the gene or advances in proteomics, the ability to supply large quantities of and bioreactors, allows GenScript to provide proteins in gram synthetic gene of interest into a pure and active proteins has become essential for the levels to its clients in both public and private research sectors, I. Gene optimization, expression • Verified sequence file mammalian cell expression vector production of protein drugs and to the development of drug academia, and all levels of the pharmaceutical industry. construct design and generation. • Procedures documentation • Subcloned gene verificaiton by qPCR assays and research methods. GenScript is fully equipped to GenScript has delivered more than 500 different kinds of and sequencing offer large-scale protein production service. On top of proteins and for one project 300 grams of protein. large-scale purification service, our versatile and reliable protein • Transfection of target cells (293 or CHO) with recombinant construct DNA • Expression data II. Transient protein • Transient protein expression • Transient protein expression expression • Expression assessment of the • Conditioned media Capabilities of Different Expression Systems: recombinant protein
Bio-Reagent Center • Establishment of stable cell line BacterialYeast Baculovirus/Insect Cells Mammalian Cells III. Small-scale • Pilot expression analysis and optimization 500 L 100 L 500 L protein expression • Generation of cell bank 1,000 L TM TM • Small-scale expression from one to five liters • Cell pellet or medium samples Fermentor Fermentor Wave Mixer Wave Mixer • Expression data IV. Large-scale • Cell harvest protein production • Large-scale production
• Protein purification by affinity column, ion Capabilities Specifications: • Purified proteins exchange, gel filtration, or hydrophobic V. Protein purification and • QC data column Expression System Capabilities Equipment characterization • Procedure • Characterization by Western blot, documentation Bacteria • Transformed bacterial culture for Fermentor SDS-PAGE, HPLC, IEF, etc. large-scale fermentation, up to 1,000 L • Homogeneous recombinant proteins over 98% pure for crystallization
• Partially purified recombinant proteins Cus
s Pricing over 70% pure t
• Large quantities of protein up to grams o
Cat. No. Service Price m Pr ervice
S SC1259 Mammalian Cell Protein Expression Services Quote Yeast • Yeast culture up to 500 L for large-scale
fermentation o t e
• Homogeneous recombinant proteins over in
98% pure for crystallization Se • Partially purified recombinant proteins Quotations and Ordering: rvi
tom Protein over 70% pure ces s
u • Large quantities of protein up to grams
C Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose BioProcess Services, the most convenient ordering method. For sample submission if applicable, please send to GenScript, 120 Baculovirus/Insect Cells • High titer recombinant baculovirus stock Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. carrying the customer's gene, up to 109 pfu/ml • Homogeneous recombinant proteins over 98% pure for crystallization Quotation Form: Obtainable on our website • Partially purified recombinant proteins TM over 70% pure Wave Mixer Email: [email protected] • Large-scale protein production option up to few hundreds of mg, using insect cells Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 and WaveTM Mixer system
Mammalian Cells • One-stop services for the production of Fax: 1-732-210-0262; 1-732-885-5878 recombinant proteins, from gene synthesis to expression, for orders of up Secured Web Server: https://www.genscript.com/ssl-bin/secure_data to 100 mg • Permits transient expression of customers' proteins of interest, up to 10 mg in 2–3 weeks • Provides conditioned media and cell pellets from stable cell lines up to 500 L, using WaveTM Mixer system
- 41 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 42 - Protein Purification and Characterization
Bacterial Fermentation Service (Cat. No. SC1027): (http://www.genscript.com/custom_protein_purification_characterization.html) Bio-Reagent Center
Having large quantities of highly purified protein is a Services: GenScript offers several methods for protein purification and 2. GST prerequisite for research involving high throughput screening, GenScript's bacterial fermentation services are highly characterization. We deliver proteins in different purity levels, 3. MBP functional analysis, and structural biology. Large-scale protein customizable. Expression and growth condition can be allowing customers to choose the ideal level of purity for their 4. FLAG production and purification often become the bottlenecks for optimized for large-scale production, purification and intended downstream applications. The options are available • Protease for Tag Removal both core facilities and individual scientists’ protein work flow. characterization. Our bacterial fermentation capacity is as high for projects involving both small and large quantities of proteins. 1. Thrombin as 1,000 L. We can produce transformed bacterial cell paste up GenScript routinely maintains stringent QC and abides by its 2. Enterokinase GenScript offers industrial-scale bacterial fermentation service to three metric tons, over 98% pure homogeneous recombinant SOPs on its protein purification processes. In addition, before 3. rTev as a solution to this bottleneck. Our service is highly proteins up to grams. shipping, our protein expression experts characterize each 4. PreScission customizable to any specific project requirement. The capacity target protein to make sure that it satisfies all of the customers' 5. Factor Xa of our bacterial fermentation service is as high as 1,000 L. Sample Requirements: stated requirements. GenScript prefers transformed bacterial culture as starting Protein Characterization: Competitive Advantages: materials for our fermentation service. Please provide detailed Protein Purification Options: Bio-Reagent Center • SDS-PAGE and Coomassie Blue staining • High capacities: GenScript's bacterial fermentation service is instructions on fermentation conditions using our service order • Chromatography • Protein concentration determination available in scales ranging between 10 and 1,000 L. form. If starting material is an expression vector DNA, or if 1. Ion-exchange • Western blot • Large-scale purification: GenScript provides comprehensive fermentation condition optimization is required, additional fee 2. Hydrophobic interaction • Absorption spectrum large-scale purification services that utilize affinity column, applies. 3. Affinity gel filtration, ion exchange, and hydrophobic-interaction 4. Size exclusion column. Delivery Specifications: • Purification Tags • Comprehensive package: GenScript offers a full range of • Cell pastes 1. 6XHis upstream and downstream services, from gene synthesis • Batch records and expression vector construction to protein purification • QC datasheets and characterization. • Competitive prices: Our bacterial fermentation service offers the most competitive prices in the industry. Quotations and Ordering:
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose
the most convenient ordering method. For sample submission if applicable, please send to Protein Services, GenScript, 120 Cus s Pricing Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. t o
Cat. No. Service Price m Pr ervice
S SC1027 Bacterial Fermentation Service Quote
Quotation Form: Obtainable on our website o t e in
Email: [email protected] Se
Quotations and Ordering: rvi tom Protein Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 ces s u
C Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Fax: the most convenient ordering method. For sample submission if applicable, please send to Protein Services, GenScript, 120 1-732-210-0262; 1-732-885-5878 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
Quotation Form: Obtainable on our website
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
- 43 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 44 - - 45 Custom Peptide Services Bio-Reagent Center Technology: peptidestogether. ligationtechnologyandmicrowaveenergytoligateshort • Peptidesof Any Length:Researcherscanuseproprietary chemicalsrequiredforsynthesis. chemicalreactionrateandreducingthequantityof decreasesthecostofpeptidesynthesisbyenhancing • DecreasedCost:Microwavetechnologysignificantly peptideyieldbypreventingunwantedaggregation. • ImprovedPeptide Yield: Microwaveenergycanimprove completeeachcycleofcouplinganddeprotection. synthesiscanmarkedlyreducethetimerequiredto • HighEfficiency: The use of microwaveheatinginpeptide asshort2 AA andaslong200 AA. proprietaryligationtechnologyallowsustoproducepeptides flexiblechoiceofLPPS,SPPS,microwavetechnology, and • HighSuccessRateDelivery: The technologicalplatformwith benefits: GenScript's peptidesynthesisoffers researchersthefollowing Advantages ofGenScript’s FlexPeptide synthesized • Ligation Technology: Longpeptideupto200 AA canbe anefficient andsmartway • Microwave Technologies: Highyieldsynthesisofpeptidesin achievedhighestsynthesissuccessrate • CombinedPlatforms:FlexiblechoicesofLPPSandSPPS, GenScript’s FlexPeptide GenScript's proprietaryFlexPeptide Custom PeptideServices GenScript alsooffers large-scalecGMP-gradepeptide cosmetic peptides,andpeptidelibraryservices. platform isanidealchoiceforstandardpeptidesynthesis, and rapidhigh-throughputpeptidelibrarysynthesis,this kilograms, comprehensivelabelingandmodificationoptions, peptides permonth.Withascalerangingfrommilligramsto peptides ofupto200residuesinlengthandacapacity6,000 High Success Rate TM Standard Price Technology Includes: Structure FlexPeptide TM platformprovidescustom TM Your InnovationPartnerinDrug Discovery!
Easy Online TM Ordering EnsuresDeliveryandQuality! Competitive
peptidemodificationservicesincludingisotopelabeling( • PeptideModifications:GenScriptoffers awiderangeof acids,whichisaremarkableachievementintheindustry. technologycansynthesizepeptideaslong200amino startingat$3.20peraminoacid.GenScriptFlexPeptide qualitypeptidesatverycompetitivepricesintheindustry, • StandardCustomPeptideSynthesis:GenScriptoffers Services: Consistency, viability, anddeliveryareguaranteed. applicationsgivesusanedgeover thecompetition. cGMP-gradepeptidesfortherapeutic anddiagnostic project.Ourcomprehensiveexperience insynthesizing peptideservicewithacapacity ofuptokilogramsper • cGMP Peptides: GenScriptprovidesalarge-scalecGMP withpeptidesofanylengthandsequence,onscale. technologiesenablesGenScripttoprovideitscustomers peptidesynthesisservice. The combinationofthetwo technologywasdevelopedtocomplementourchemical • RecombinantPeptides:GenScript'srecombinantpeptide aggregativenatureofhydrophobicpeptide. serviceisdesignedtohandlelowwater-solubilityand • ClickPeptideService:GenScript'sfeaturedclickpeptide availablequalityforallcosmeticpeptides. customer'sexactspecifications.We guaranteethebest research-andcGMP-gradecosmeticpeptidestoeach • CosmeticPeptideService:GenScriptmanufactures atverycompetitiveprices. providingcustomlibrariesofunboundpeptides5–20mer high-throughputparallelpeptidesynthesisplatform, • PeptideLibraries:GenScripthasdevelopedarapid acetylation,biotin,FITC,etc. multiplephosphorylations,KLH,BSA,ovalbumin,amidation, synthesis industry. GenScript offers themostcompetitivepricesinpeptide services, andourcollectionofcatalogpeptides.Inaddition, systems offer easyaccesstoourstandardpeptidesynthesis GenScript's secureandsimpleonlinequotation/ordering production services. 15 Price N, and 13 C), PEGylation,multipledisulfidebonds, (http://www.genscript.com/peptide_overview.html) UNBEATABLE SERVICE Peptide TM 2 H,
Standard PeptideSynthesis thisfigurerisesto30%lessexpensive. counterpartfromourcompetitors. IntheEuropeanmarket, servicerepertoireisatleast20%lessexpensivethanits aslow$3.20peraminoacid.Everyitemonourpeptide • CompetitivePrices:Ourstandardpeptideservicestartsat beenwellacceptedinthecommunity. wellasourotherproductsandservices.Bothsystemshave easyaccessestoprojectstatustrackinganddatareportsas providesasecureandconvenientonlineorderingsystemfor quotationsystemforcustompeptides.GenScriptalso GenScriptisthefirstcompanytooffer aninstantonline • InstantOnlineQuotationsandEasyOrdering: contributestoourhighsuccessrate. technologyincreaseschemicalcouplingefficiency and technologytoitspeptide-manufacturingpipeline. This • Microwave Technology: GenScripthasappliedmicrowave average)successrateis75%. competitors. The industry'sgeneral(considerchangeto peptidesynthesisisover95%,whichsurpassesmanyofour • HighSuccessRate:GenScript’s successrateforcustom peptide synthesisbyincreasingchemicalcouplingefficiency FlexPeptide rate wellabovetheindustrystandard.GenScript's GenScript producesthehighest-qualitypeptideswithasuccess • Immunizationandquantitativereceptor-ligandinteractions • Biologicaleffects ofdefinedpeptides • Antibody-antigen interactions • Enzymespecificityandenzymaticmechanismstudies electrophoresis • Newtechniquesforseparationbychromatographyor • Identification/synthesisofposttranslationalmodifications • Monoclonalandpolyclonalantibodyproduction Applications: Competitive Advantages: Prices Instant onlinequotations Flat ratesforallpeptides High successrate Feature TM technologyleadstosignificantcostsavingin Email: [email protected] Tel: 1-732-885-9188 Starting at$3.20peraminoacid Instant Yes ≥ GenScript 95% technologies. We haveapassionforinnovation! science research,GenScriptaggressivelyseekstodevelopnew As oneofthepioneersinpeptideserviceindustryforlife and shorteningcouplingtime. quality control. step ofpeptidesynthesisissubjecttoGenScript'sstringent sequence, purity, quantity, andassociatedQCreports.Every Typical deliveryconsistsoflyophilizedpeptidetherequired Delivery Specifications: ofreagentfromself-allocating. unnecessaryfreeze-thawcycles,waterabsorptionandloss solubilityforyourpilotexperiment. This prevents aliquotshavebetterstabilityandcanbeusedtotestthe theamountofpeptidesasneededeachtime. The extra packagingaslittle0.2mg/vialallowsresearcherstouse • Convenient Aliquoting on Your Demand:Conveniently project. thismayvarydependingonthesizeandcomplexityof in-house.Ourtypicalturnaroundtimeis2–3weeks,though • Fast Turnaround Time: GenScriptperformsalltasks • Epitopemapping NMR • Structure,dynamics,andfoldingofpeptidesproteinsvia • Non-quantitativepeptideblockingstudies • Non-quantitativeenzyme-substratestudies • ELISA standardsformeasuringantibodytiter • Blockingandcompetitionproteaseassay • Immunizationandquantitativereceptor-ligandinteractions (http://www.genscript.com/peptide.html) Competitor 20% higher Day (s) ≤ 75% No
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Bio-Reagent Center Bio-Reagent s Service tide p Pe Custom - 47 Custom Peptide Services Bio-Reagent Center Quotations andOrdering: Peptide Modifications Centennial Ave., Piscataway, NJ08854,U.S.Pleasecompleteandenclosethequotationform. the mostconvenientorderingmethod.Forsamplesubmissionifapplicable,pleasesendto Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech seven-atom aminohexanoylspacer(NH fluorescein labeling.Forefficient N-terminallabeling,a Fluorescein isothiocyanate(FITC)isanactivatedprecursorfor is thenremoved. side chainviaanamidebond. The positivechargeofthelysine C-terminus ofthepeptide.Biotinisthenattachedtolysine For C-terminalbiotinlabeling,aLys residueisaddedtothe Biotin andFITC degradation. peptide morestabletowardexopeptidasesenzymatic CONH its chargeandhelpitimitatenaturalstructure(amide, amidation (Cterminus)oracetylation(Nwillremove If thepeptideisfromaninternalsequenceofaprotein,terminal Amidation and Acetylation GenScript offers awiderangeofpeptidemodificationservices. peptide. able tointroduceupthreecustomized disulfidebridgesinone cysteine atspecifiedpositionsin a controlledprocess.We are them. GenScriptisabletobuilddisulfide bridgesbetween residues duetorandomformations ofdisulfidebridgesbetween challenging practiceforpeptidecontainingmultiplecysteine bridges betweencysteineresiduesonthepeptide. This isa Peptide cyclizationcanbeachievedbyintroducingdisulfide Multiple DisulfideBonds (fluoroscein) andtheN-terminusofpeptide. CH 2 -CH 2 ). Inaddition,thismodificationmakesthemodified 2 Secured Web Server: Fax: Phone: Email: Quotation Form: Secured OnlineQuotation: -COOH) isinsertedbetweenthefluorophore 1-732-210-0262; 1-732-885-5878 [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 Obtainable onourwebsite https://www.genscript.com/ssl-bin/secure_data https://www.genscript.com/ssl-bin/order_peptide_chemical 2 -CH 2 -CH 2 Your InnovationPartner inDrugDiscovery! -CH 2 - hydrophilic. The PEGylatedmacromoleculeshaveenhanced polymers thatarenonionic,nontoxic, biocompatible,andhighly (antibody, peptide,etc.)with polyethyleneglycol(PEG), PEGylation isthecovalentconjugation ofmacromolecules PEGylation Note: KLHisalarge(M.W. =4×10 carrier protein. or Cterminusofthepeptidesothatitmaybelinkedto perform thisconjugation,onecysteineresidueisaddedtotheN the cysteineresidueofpeptidetocarrierprotein. To conjugation methodisthemaleimidemethod,whichcouples not usedasablockingreagent.Onecommonmeansof does notinterferewithELISA orWestern blottingbecauseitis (BSA), andovalbumin.OneoftheadvantagesKLHisthatit as keyholelimpethemocyanin(KLH),bovineserumalbumin these peptidesareconjugatedtobiggercarrierproteins,such immune responsesontheirown. To circumvent this drawback, Peptide antigensareoftentoosmalltogeneratesignificant KLH, BSA,andOvalbumin protection orbyFmoc-protectedphosphorylatedaminoacids. selective phosphorylationcanbeachievedbyorthogonal peptides containingoneormoreofthesehydroxy-aminoacids, serine-, threonine-,andtyrosine-phosphopeptides.For kinases. GenScripthassuccessfullysynthesizednumerous and elucidatetheregulatoryprocessesmediatedbyprotein influences ofphosphorylationonpeptidesandproteinstructure Phosphopeptides canassistintheinvestigationof Multiple Phosphorylations immunogenicity andtheturbidsolutioncanbeusedforimmunizations. giving solutionsandmixturesacloudyappearance. This doesnotaffect Because ofitssizeandstructure,solubilityinwaterisoftenlimited, (http://www.genscript.com/peptide_modification.html) Peptide Services 5 to1×10 , GenScript,120 7 ) aggregatingprotein. oose the mostconvenientorderingmethod. Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech number, eachlibraryisgeneratedbybreaking theoriginal Characterized bytwoparameters, fragmentlengthandoffset essential aminoacidsthatcontribute toitsfunctionality. out whichpartofagivenproteinor peptidecontainsthe continuous epitopemapping,which caninturnbeusedtofigure Overlapping peptidelibrarycanbe usedforlinearand Overlapping PeptideLibrary: Peptide LibrariesandScreeningTools epitope mappingandsequenceoptimization. library generationprotocols. They canbeefficiently usedin peptide synthesisplatformequippedwithsixpowerful GenScript hasdevelopedarapidhigh-throughputparallel peptide/protein crosstalking,etc. epitope screening,discoveryofsignalmolecules, binding, enzymesubstrateorinhibitorscreening,antigenand protein-protein interaction,functionalproteome,nucleicacid including drugdiscovery, GPCRligandscreening, fast-growing fieldofproteomicsanditsrelatedsubfields Peptide librarieshavebecomeacommandingassetinthe Custom PeptideLibraryServices Quotations andOrdering: both nonradioactive isotopes.Peptideslabeledwith For NMRmeasurement,wecanlabelpeptideswithstable Isotope Labeling within hostthroughreducedrenalclearance. minimum immuneresponseinhost,prolongedcirculatorytime hydrophobic drugs)andbioavailability, maskedantigenicityfor therapeutic propertiesduetotheirincreasedsolubility(for customized labelingofyourpeptides. research. Pleasesubmityoursequenceandrequestfora 15 N and Secured Web Server: Fax: Phone: Email: Secured OnlineQuotation: 1-732-210-0262; 1-732-885-5878 13 C canbesynthesizedforconvenientdetectionin [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 https://www.genscript.com/ssl-bin/secure_data https://www.genscript.com/ssl-bin/order_peptide_chemical Email: [email protected] Tel: 1-732-885-9188 2 H, 15 N, 13 C, or vaccine development. determination intheareasofinfectious diseases,oncology, and example, thelibrarycanbeused for the T-cell epitope The overlappingpeptidelibraryhas manyapplications.For fragments. number andlongfragmentlengthproducestheleastof number offragmentswhilethecombinationhighoffset offset numberandshortfragmentlengthgeneratesthemost the fragmentstocoveranepitope. The combinationoflow the librarygeneratesfewerfragments. Also, itismorelikely for length. Usually, longerfragmentsaredifficult tosynthesizebut The offset numberisusuallychosentobe1/3ofthefragment can minimizetheexperimentcostswhilemaximizingdatavalue. Careful selectionsoftheoffset numberandthefragmentlength the degreeofoverlap. acid residuesshiftedbetweenadjacentfragmentsanditreflects cover anepitope. The offset numberistheofamino peptide fragmentmustbeatleastsixresiduesinlengthforitto each has8to20residuesinsize. As ageneralguideline, protein orpeptideintomanyequal-lengthoverlappingfragment, eliminates theneedtoconjugateantigenaproteincarrier. numbers ofpeptidebranchescanbesynthesized. This be attached.Dependingonthenumberoflysinetiers,different lysine toformabackbonewhichmultiplepeptidechainscan vaccines. This systemutilizesthe produce high-titeranti-peptideantibodiesandsyntheticpeptide Multiple antigenpeptide(MAP)applicationisonepotentwayto MAPs Application (http://www.genscript.com/peptide_library.html) α - and ε -amino groupsof oose
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Bio-Reagent Center Bio-Reagent s ervice S Peptide ustom C - 49 Custom Peptide Services Bio-Reagent Center peptides, receptorligands,antimicrobial compounds,and used toscreenhighlyactivecompounds suchasantigenic become keyplayersindrugdiscovery. These libraries canbe Combinatorial peptidelibrariesand HTScompoundarrayshave DNAs, etc. target moleculesofinterestincludingproteins,antibodies, scenario forsequenceoptimization.Itcanbeusedtoprobe alternative peptides,thescrambledlibrarycreatesanideal permutation oftheoriginalpeptide.Representingallpossible peptide library. The libraryisconstructedthroughsequence Scrambled librarybringsthehighestdegreeofvariabilityfor Scrambled Library: the selectedaminoacidresidues. random libraryfabricatesasmanyvariationspossiblewithin all other20naturalaminoacidsviaashotgunapproach.Our substitute selectedresiduesrandomlyandsimultaneouslywith that couldhavethepotentialforenhancedactivity. We optimization. Ithastheabilitytogeneratealternativepeptides Random libraryisanindispensabletoolforsequence Random library: synthesis andscreening. substrates withinterdependentsubsiteonlyminimum addition, thistypeoflibrarycanalsobeusedtolocate identify T-cell epitopesfromcomplexmixturesofproteins.In In particular, positionalscanninglibraryhasbeenusedto residue(s) atspecifiedposition(s)forenhancedpeptideactivity. generates highvaluedatabylocatingpotentialmorefavorable that positionwithallothernaturalaminoacidsoneatatime.It given positionorpositionsandsubstitutestheaminoacid(s)at sequence optimization.Itidentifiesaminoacidsofinterestata Positional scanninglibraryisanimportanttoolforpeptide Positional ScanningLibrary: drugs tothemarket. degradation, whichisamajorinhibitingfactorbringingthese as atooltoinvestigatepeptidedrugsundergometabolic about thepeptideswithenhancedproteolyticstability. Itcan act In manycases,truncationlibraryscreeninggivesknowledge be centeredaroundthem. Alanine ScanningLibrarystudies,thetruncationfragmentscan each terminus.Knowingthepositionsofkeyresiduesvia through asystematictruncationofthepeptide'ssequencefrom length requiredforepitopeactivity. The libraryisgenerated Truncation libraryallowsresearcherstodeterminetheminimum Truncation Library: contribution tothepeptide'sfunctionality. us toquicklydetermineeachindividualaminoacid's alanine causesdiminishedepitopeactivity. This libraryenables measured. Substitutionofkeyaminoacidresidue(s)with Subsequently, correspondingchangeinepitopeactivitycanbe to substituteeachnon-alanineresidueoneatatime. conformation. Alanine, thesmallestchiralaminoacid,isused residues responsibleforthepeptide’s function,stability, and Alanine scanninglibraryisabletoidentifyspecificaminoacid Alanine ScanningLibrary: Your InnovationPartner inDrugDiscovery! • Kinaseassays • Customizedpeptidemicroarrayproduction • High-throughputprotein-proteininteractionanalysis • Vaccine research • Epitopemappingstudies screening toolsareasfollows: enzyme inhibitors.Someoftheapplicationspeptide • Modificationsandotherspecifications • Numberofoverlappingresiduesifrelevant • Minimumquantityandpurityrequirements • Lengthandsequenceofeachpeptide • Numberofpeptidesdesired body ofthemessage,pleaseincludefollowing: (1-732-210-0262), orviaoursecuremessagingsystem.Inthe ([email protected]), phone(1-732-885-9188),fax For quotations,pleasecontactusbyemail • MSandHPLCdata • Peptidelocationtable • Lyophilized unboundpeptidesin96-wellformat The typicaldeliveryconsistsof: Delivery Specifications: • 2–3weekstypicalturnaroundtime aminoacid,etc. • Modificationsincludingbiotin,fluorescence,unnatural • Peptidelengthof5–20mer • 0.5–2.0mgforeachcrudepeptide • Cat.No.SC1177 specifications ofourlibraryservicesare: with minimumorderofoneplate48peptides. The including epitopemapping.Ourlibraryservicesareavailable GenScript peptidelibrariesaresuitableforallapplications, Service Specifications: validationdataforeachpeptide. • StringentQualityControl:GenScriptprovidesMSandHPLC 96-wellplates. • Ready-to-UseFormat:GenScriptdeliverspeptidelibrariesin andpeptidecyclizationwithdisulfidebridges. includeslabeling,theincorporationofunnaturalaminoacids, • ComprehensiveModifications:Ourmodificationservice thewellsof96-wellplates. • NoCross-Contamination:Peptidesaredirectlylyophilizedin purity, andquantitybatch-to-batchwithineachbatch. usesoptimizedchemistriestoensureconsistentquality, • OptimizedChemistries:GenScriptpeptidelibrarysynthesis Key Features: Quotations andOrdering: the mostconvenientorderingmethod. Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech Pricing: 2. The amountandpuritymayvarydependingonthesequencesinvolved. 1. FormodificationsotherthanN'biotin,suchasfluorescence,unnaturalaminoacid,etc.,pleaserequestaquotation. Notes: SC1177 Cat. No. Secured Web Server: Fax: Phone: Email: Secured OnlineQuotation: 1-732-210-0262; 1-732-885-5878 [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 Quote Prices https://www.genscript.com/ssl-bin/secure_data https://www.genscript.com/ssl-bin/order_peptide_chemical Email: [email protected] Tel: 1-732-885-9188 Quote N-Biotin 1 0.5–2.0 mg,crude Amount &Purity 2 All samples QC Turnaround 2–3 weeks oose
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Bio-Reagent Center Bio-Reagent s ervice S Peptide ustom C - 51 Custom Peptide Services Bio-Reagent Center Quotations andOrdering: cosmeticpeptidesathighlycompetitiveprices. • Prices:GenScriptprovideslargescaleproductionof weightasdeterminedbyaminoacidanalysisand/orUV. • Content:Ourcosmeticpeptidesaresoldbynetpeptide pharmaceuticalandbiotechnologyapplications. research-andcGMP-gradecosmeticpeptidesforarangeof • Applicability: GenScriptprovidescustomandgeneric • Consistency:Batch-to-batchconsistencyisguaranteed. andanti-wrinklepropertiesofeachpeptide. optionsthatcanimprovetheskinpermeating,anti-aging, • Modification:We provideanarrayofpeptidemodification needs. upto98%.Puritylevelscanbetailoredeachcustomer's • Quantity:Batchsizerangesfrommgtokgwithpuritylevel Key Features: quality ensureastrongpartnershipforsuccess. stringent QCtogetherwithourdedicationtoinnovationand exact specifications.GenScript'stechnicalexpertiseand research- andcGMP-gradecosmeticpeptidestoeachclient's and instrumentationavailable,GenScriptcanmanufacture By employingthemostadvancedpeptidesynthesistechniques over theworld. and dermaceuticalsbeingdevelopedbyskincarecompaniesall skin agingandthelatestadditiontoranksofcosmeceuticals Cosmetic peptidesarethenewweaponinbattleagainst technologically advancedcompaniesincosmeticindustry. mediate keybiologicalfunctions,hasattractedthemost The intrinsicpropertiesofpeptides,particularlytheirabilityto Cosmetic Peptides the mostconvenientorderingmethod. Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech Secured Web Server: Fax: Phone: Email: 1-732-210-0262; 1-732-885-5878 [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 https://www.genscript.com/ssl-bin/secure_data Your InnovationPartnerinDrug Discovery! • Bioburden • Inorganicflouride(ifappropriate) • Trifluoroacetic acid • Residualorganicsolvents • Specificrotation • Massbalance • Water • Counterioncontent • Bacterialendotoxins • Assay (bypeptidecontent) • Relatedsubstances(byHPLC) • Identity(byaminoacidanalysisandHPLC) charges: listed analysiscanbeprovideduponrequestwithadditional Spectral analysis,andpeptidepurity(byHPLC). The following standard COA containsappearanceinformation,solubility, Mass Every batchofproductcomeswithalot-specificCOA.Our Practice (GMP/GLP) Quality controlaccordingtoGoodManufacturing&Laboratory • Batchrecordsforeachstepinthemanufacturingprocess • Certificatesofanalysisforallrawmaterials • Batchtraceabilityofallrawmaterials • Equipmentvalidation Full supportforFDA submissions: product. sheets correspondingtoeachbatcharesentwithevery identification andveryhighpurity. Specificanalyticaldata follow stringent,preciseprocedurestoensurecomplete GenScript guaranteestopqualityforallcosmeticpeptides.We Quality Control: (http://www.genscript.com/cosmetic_peptides.html)
oose Quotations andOrdering: biologicexperiment • Noby-productintheconversionprocess,suitablefor • The conversionisaonewayandquickprocess easilyatpH7.4orabove. • The "clickpeptide"(precursor)convertstonativepeptide an exogenousaction(“click”) 2. Converttothenativepeptide(hydrophobicpeptide)insituby enzyme-substratebindingaffinity) • Biologicactivity(e.g.,ligand-receptorbindingaffinity, or self-assembly, aggregation,orfolding). • Physicochemicalproperty(e.g.,water-solubility, 1. Controlthefollowingnaturesofhydrophobicpeptide Key Features: aggregative natureofhydrophobicpeptide. (SC1506) isdesignedtohandlelowwater-solubilityand experiments. GenScript'sfeaturedclickpeptideservice proper dissolvingofpeptidesposesadifficulty priortheirassay process. Eventhepeptidescanbesuccessfullyprepared, The preparationofhydrophobicpeptidesisatimeconsuming Click PeptideService the mostconvenientorderingmethod. Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech Secured Web Server: Fax: Phone: Email: Secured OnlineQuotation: 1-732-210-0262; 1-732-885-5878 [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 https://www.genscript.com/ssl-bin/secure_data https://www.genscript.com/ssl-bin/order_peptide_chemical Email: [email protected] Tel: 1-732-885-9188 • Applicable tocellsignaltransductionandotherstudies peptide1–42(E22 • HuiWang, Taeko Kakizawa,etal.Synthesisofamyloid Nov2008;9(18):3055-3065 Water-Soluble Precursor"ClickPeptide".ChemBioChem. Productionof Amyloid • Youhei Sohma, Atsuhiko Taniguchi, etal.Controlled 823-828 ChemicalBiology-orientedanaloguesof Alzheimer's amyloid • Atsuhiko Taniguchi, Youhei Sohma,etal.Clickpeptide: References: biologicalfunctionsof • Provideanovelsystemusefulforinvestigatingthedynamic • Slowtheaggregationdramatically • Increasethesolubilitysignificantlyinyourresearchuse Key Advantages: Chemistry. July2009;17(14):4881-4887 productionofitsnativeform.Bioorganic&Medicinal β peptide1–42.J.PeptideScience.Nov2006;12(12): (http://www.genscript.com/click_peptide_service.html) Δ ) clickpeptide:pH-triggeredinsitu β β -amyloid (1-42)in AD PeptidefromaPhoto-Triggered, oose β
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Bio-Reagent Center Bio-Reagent s Service tide p Pe Custom - 53 Custom Peptide Services Bio-Reagent Center sequences. peptidetechnologycanhandlethetoughestpossible complicatedpeptides,whereasourproprietaryrecombinant synthesishaslimitedabilitytosynthesizelongor • OutstandingProcedure:Conventionalchemicalpeptide customer'sprecisespecificationsofsequenceandpurity. stringentQCguaranteesthateachdeliverymatchesthe providescustompeptidesexactlyasrequested.Our • HighPrecision:GenScript'srecombinantpeptidetechnology Key Features: customers withanypeptide,oflength,onscale. of thesetwotechnologiesallowsGenScripttoprovideits chemical peptidesynthesisservice. The powerfulcombination recombinant peptidesystemasacomplementtoitsstandard synthesize chemically. GenScripthasdevelopedaproprietary peptides (multipledisulfidebonds)areprohibitivelyexpensiveto However, longpeptides(>150residues)orcomplicated Most peptidescanbesynthesizedbychemicalmeans. Custom RecombinantPeptides Quotations andOrdering: the mostconvenientorderingmethod. Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech Secured Web Server: Fax: Phone: Email: 1-732-210-0262; 1-732-885-5878 [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 https://www.genscript.com/ssl-bin/secure_data Your InnovationPartner inDrugDiscovery! consistency, timeaftertime. technologyproducessyntheticpeptideswithunbelievable conventionalpeptidesynthesis,recombinant • Batch-to-BatchConsistency:Withfewerstepsthan expenses. peptidesmayberegenerated,furtherdecreasinglong-run complicatedpeptideproduction.Inaddition,recombinant cost-effective approachforlarge-scale,long,and • LowCost:Ourrecombinantpeptidepackageprovidesa kilograms. • ScalableSystem:Orderscanrangefrommilligramsto • • • • Applications:
Exploreclinicalapplications Performpeptidedrugresearch Performpathologicalresearch Synthesizeantigensforantibodyproduction (http://www.genscript.com/recombinant_pep.html) oose at serine,threonine,ortyrosine),amidation,acetylation, cGMP peptides. These includeglycosylation,phosphorylation We havethecapacitytointroducearangeofmodifications strictly obeyed. raw materialhandlingandtestinghavebeendevelopedare COAs canbeincludedintheproductdocumentation.SOPsfor We constantlyauditourrawmaterialsuppliers.Raw help youachieveyourgoals. experienced scientistswillworkonacustomizedprotocolto your peptideisdifficult tosynthesizebytraditionalmethods,our cost-effective manufacturingprocessforeachspecific API. If counterions, andsolventstoensurethemostsuitable strategies, protectinggroupcombinations,activationmethods, preliminary methoddevelopmenttofindoptimumsynthetic Before conductinglarge-scalepeptidesynthesis,weconduct your specificationsforeachproduct. adjust thepurityandsingleimpuritypercentageaccordingto more aminoacidsinlength,higherthan99%purity. We can milligrams toupkilograms. These peptidesmaybe50or Our facilitycansynthesizepeptidebatchesfromafew cGMP PeptideManufacturing Process marketed asdrugsandhundredsmoreareindifferent stagesof and moreprevalent.Dozensofpeptideshavealreadybeen The useofpeptidesinclinicalapplicationsisbecomingmore cGMP PeptideSynthesis Customized solutions Proven trackrecord Advanced FlexPeptide Service Includes TM technology Email: [email protected] Tel: 1-732-885-9188 FlexPeptide Key Feature Priced foranybudget Designated projectcoordinator Personalized attentiontoprojectsofallsizes Strict rawmaterialsscreening Advanced testingtechniques Fully equippedcGMP facility Cost-efficient synthesismethods Peptides upto50 AA ormore Batch sizefrommgtokg Peptide modifications • Othertestsasrequired • Thin-layer chromatography • Organicvolatileimpuritiesbygaschromatography(GC) • Endotoxinlevels • Trifluoracetic acid(TFA) content • Residualorganicsolventcontent • Water content • Counterioncontent • Peptidecontent • Molecularweightbymassspectrometry • PuritybygradientHPLC • Solubility • Appearance characteristics: For qualitycontrolofthefinishedproduct,wetestfollowing and sizeexclusionchromatography. several othermethodsareavailable,includingionexchange Our usualpurificationmethodisreversephaseHPLC,but multi-antigen peptidesynthesis. biotinylation, conjugationtoFITC,ovalbumin,KLHorBSA,and earliest stagesofyourproject. patients faster, youshouldusecGMP-gradeproductsfromthe clinical development.Ifyouwantyourpeptideideatoreachthe TM technology (http://www.genscript.com/GMP_peptide.html)
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Bio-Reagent Center Bio-Reagent s ervice S Peptide ustom C - 55 Custom Peptide Services Bio-Reagent Center Quotations andOrdering: Centennial Ave., Piscataway, NJ08854,U.S.Pleasecompleteandenclosethequotationform. the mostconvenientorderingmethod.Forsamplesubmissionifapplicable,pleasesendto Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech Secured Web Server: Fax: Phone: Email: Quotation Form: 1-732-210-0262; 1-732-885-5878 [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 Obtainable onourwebsite https://www.genscript.com/ssl-bin/secure_data Your InnovationPartner inDrugDiscovery! Peptide Services , GenScript,120 oose Quotations andOrdering: activity studies.Peptideswithpuritygreaterthan95%are greater than85%areusuallyusedinenzymologyorbiological generating ortestingantibodies.Peptideswithpuritylevel Peptides withpuritygreaterthan70%arealwaysusedfor levels ofpurityforourcustompeptidesynthesisservices. make therightchoiceforyourapplication.We proposefour GenScript proposesarangeofdifferent puritylevelstohelpyou Peptide PurityandApplication the mostconvenientorderingmethod. Quotations andordersmaybeplacedbyemail,phone,orfaxwitheitheraformalPO(PurchaseOrder)creditcard.Pleasech Secured Web Server: Fax: Phone: Email: Secured OnlineQuotation: 1-732-210-0262; 1-732-885-5878 Peptide purity>98% Industrial GradePeptide Peptide purity>95% High PurityGradePeptide Peptide purity>85% Biochemistry GradePeptide Peptide purity>70% Immunograde Peptide Recommended Purity [email protected] 1-877-436-7274 (Toll-Free); 1-732-885-9188 https://www.genscript.com/ssl-bin/secure_data https://www.genscript.com/ssl-bin/order_peptide_chemical Email: [email protected] Tel: 1-732-885-9188 • SARstudies • Clinicaltrials • Cosmeticpeptidesforcosmeceuticalsindustry • cGMP peptidesfordrug studies • Generatingstandardcurves • • • NMRstudies • Quantitativeproteolysisstudies • Quantitativephosphorylationstudies • Quantitativeblockingandcompetitiveinhibitionassays • Quantitativereceptor-ligandinteractionstudies • Cellattachmentstudies • • PeptideblockingstudiesbyWestern blotting • Phosphorylationstudies • Semi-quantitativeenzyme-substratestudies • Epitopemapping • Peptidearray • ELISA tests antibodies(OnlyGenScriptoffers thisserviceatthepurityof>85%) • Antigen forproductionandaffinity purificationofpolyclonal Applications In-vivo In-vitro In-vitro studies delivery. Spectrometry ofwhichtheQCdataisincludedinpeptide The qualityofthepeptidesisverifiedbybothHPLCandMass commercial applicationsforourindustrialcustomers. peptides withpuritygreaterthan98%inlargequantities excellent forquantitativeanalysis.We alsoprovidehighpurity bioassays bioassays (http://www.genscript.com/recommended_peptide_purity.html) oose
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Bio-Reagent Center Bio-Reagent s ervice S Peptide ustom C Custom Antibody Services
(http://www.genscript.com/antibody.html) Advanced Antigen Design: GenScript’s OptimumAntigenTM quality antibodies that generate positive Western blot results. Antigen Design Tool combines the advanced algorithms with Bio-Reagent Center GenScript's time-tested expertise to provide peptide antigens For polyclonal antibody projects that use peptide antigens A reliable analysis of biological materials demands reliable We guarantee: that produce quality antibodies. designed, synthesized, and conjugated by GenScript, we antibodies and antibody services. These in turn demand guarantee a final antibody product with an ELISA titer of industry leading technologies and experienced, professional • For antibody product generated in any host using any Nanotechnology for Antigen Preparation: Unlike those of 1:20,000 or better. staff. With state-of-the-art techniques and dedicated specialists, qualified antigen, we guarantee ELISA titer 1:20,000 or many other antibody production companies, GenScript antigen GenScript provides a one-stop solution to all its customers' better. mixtures are processed down to nanoparticles to maximize Competitive Prices: Our polyclonal antibody service starts as antibody needs. Our services include recombinant protein • For Monoclonal Antibody product generated in any host immunization efficiency and boost immune response. low as $795.00. Our monoclonal antibody service starts as low expression, peptide design and synthesis, polyclonal and using any qualified antigen, we guarantee two positive as $3,800.00. Our express polyclonal antibody service delivers monoclonal antibody production, affinity purification, conjugation, clones. T-MaxTM Adjuvant: T-MaxTM adjuvant is significantly more antibody in 5–8 weeks. fragmentation, and immunoassay development. Our animal • For both Polyclonal and Monoclonal antibody product potent in inducing the immune reaction than the standard facility is AAALAC International accredited. generated using qualified protein antigen, we guarantee Freund’s adjuvant, which ensures high specificity and high titer Comprehensive Package: GenScript's one-stop antibody positive Western blot results. of the produced antibodies. services are tailored to each customer's unique needs. For
Bio-Reagent Center Bio-Reagent example, the process of antigen design by OptimumAntigenTM Antibody Lyophilization: Lyophilization extends the shelf life design tool and carrier protein selection are dictated in part by of your antibodies, eliminates leaking problems, loosens up the customer's intended final application. In addition, we adjust stringent temperature requirement during shipping, and reduces the immunization dosage and number of booster immunizations Acclaimed Guaranteed Comprehensive Competitive UNBEATABLE the overall weight of antibodies to be shipped. All antisera or according to the titer of initial antisera from each animal. Antigen Design Immune Package Prices SERVICE Response purified antibodies are provided as lyophilized form except special requirements.
Guaranteed Immune Response: We use Western-GUARANTEED peptide polyclonal antibody package to satisfy your need for Antigen design
Immunogen preparation
Polyclonal antibody production Rabbit, mouse, rat, goat and chicken C s ustom Antibod Antibody development Custom Polyclonal Antibody Services Antigen-based affinity Monoclonal antibody production Mouse in vitro and in vivo production Protein A/G/L (http://www.genscript.com/polyclonal_antibody_service.html) Service
y Purification options lon-exchange Other methods Unlike monoclonal antibodies, polyclonal antibodies correspond were designed, synthesized, and conjugated by GenScript. For
Fragmentation y
Modification Conjugation to biotin, enzymes, and fluorescent dyes to multiple epitopes and tend to contain several immunoglobulin any protein antigen (3 mg, > 80% purity, 90% preferred) we S subtypes. They can be used in Western blotting as primary and guarantee a polyclonal antibody with positive Western blot ervice Western blot secondary antibodies, and in ELISAs and IHC. We guarantee results and an ELISA titer of 1:20,000 or better for any host. ELISA Characterization via a broad range of methods
that your polyclonal antibody produced by us in any host will Or we will restart the project free of charge until the guarantee s
Custom Antibod IHC IP have an ELISA titer of 1:20,000 or better if peptide antigens is met. Our animal facility is AAALAC International accredited. Validated Immunoassay development Through-put format Assay kit available-easy to use
Competitive Advantages: Polyclonal Antibody Services
Feature GenScript Competitors Advanced antigen design (OptimumAntigenTM) Yes No GLNTRKKLQ + R2 Antibody H N C | 3 || C cys O KLH Nanotechnology for antigen preparation Yes No CH NH C | || O T-MaxTM adjuvant Yes No R1 Antibody lyophilization Yes No Antigen design Peptide synthesis Animal Antiserum Purification QC by Delivery of Guaranteed Western and coupling immunization preparation Western blot / ELISA antibody product Guaranteed immune response (Western-GUARANTEED peptide polyclonal antibody package) Immune 1:20,000 ELISA titer (polyclonal antibody) Rare Two ELISA positive clones (monoclonal antibody) Polyclonal starts at $795.00 Prices 30% or higher Monoclonal starts at $3,800.00
- 57 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 58 - Service Packages: SC1247: Basic Affinity-Purified Polyclonal Antibody Package (Rabbit) Bio-Reagent Center
GenScript offers a variety of polyclonal antibody production packages. Our experienced sales account managers will guide you toward Service Package Peptide Antigen Protein Antigen the best selection for your specific needs. • Protein checking by SDS-PAGE • Two rabbits, pre-immune bleed, Service packages Package Includes Deliverables four immunizations/rabbit • Peptide synthesis • 3–5 mg affinity-purified antibodies • Conjugation to KLH • Final bleed SC1031: • Two rabbits, pre-immune bleed, (lyophilized) • Two rabbits, pre-immune bleed, • Protein conjugated to affinity resin Complete Affinity-Purified Peptide four immunizations and final bleed • 5 mg of unconjugated peptide four immunizations/rabbit (Dialyze the protein with PBS if needed) Polyclonal Antibody Package (Rabbit) • Peptide affinity purification • 1–2 ml of pre-immune serum (lyophilized)* Includes • Final bleed • Affinity purification of antibodies (Most Popular) • ELISA test • ELISA, MS and HPLC results • Peptide conjugated to affinity resin • ELISA test • Lyophilization • Total time: 15–17 weeks • Affinity purification of antibodies • Lyophilization • ELISA test • Antigen provided by the customer: • Peptide design and synthesis • 3–5 mg purified antibodies • Lyophilization 7.5–8 mg of protein (2.5–3 mg for Bio-Reagent Center • Peptide-KLH conjugation (lyophilized) from 2 best rabbits • Antigen provided by the customer: immunization, 5 mg for purification). SC1390: • 6 rabbits, pre-immune bleeds and test bleeds • 5 mg peptide 7.5–8 mg of peptide (2.5–3 mg for immunization, The concentration should be > 0.4 mg/ml, Western-GUARANTEED Peptide • Affinity purification and ELISA results • 1–2 ml of pre-immune serum (lyophilized)* 5 mg for purification) and no organic solvents in the antigen Polyclonal Antibody Package • Western blot test • MS and HPLC reports solution. Tris and other solution containing (Rabbit) • Lyophilization • Western blot, ELISA titer reports amino groups should not be used. • Total time: 16–18 weeks • 1–2 ml of pre-immune serum (lyophilized)* • 1–2 ml of pre-immune serum (lyophilized)* • Synthesis of two peptides, one with modification • 1–3 mg phospho-specific antibodies Delivery • ELISA results • ELISA results (10–15 AA, > 90% purity, 15 mg) and the other without (lyophilized) from two rabbits • 3–5 mg affinity-purified antibodies (lyophilized) • 3–5 mg affinity-purified antibodies (lyophilized) modification (10–15 AA, > 90% purity, 10 mg) • 5 mg of phospho-peptides and 5 mg • Remaining antigen • Remaining antigen • A cysteine is automatically added to the C terminus of unmodified-peptides (N terminus) of the peptide and conjugated to KLH. • 1–2 ml of pre-immune serum (lyophilized)* • Conjugation: 1 week • Protein checking: 1 week SC1044: • Two rabbits, pre-immune bleed, four immunizations/rabbit • ELISA results, MS and HPLC reports • Immunization: 10 weeks • Immunization: 10 weeks Phospho-Specific Polyclonal • Final bleed • Total time: 15–17 weeks Time Frame • Purification and ELISA: 1 week • Purification and ELISA: 1 week Antibody Package (Rabbit) C s • Prepare two affinity columns • Lyophilization and Shipping: 1–2 weeks • Lyophilization and Shipping: 1–2 weeks ustom Antibod • Antibody purification by modified-peptide affinity column • Total 13–14 weeks • Total 13–14 weeks and unmodified-peptide cross-adsorption.
Service • ELISA test y • Lyophilization SC1248: Basic Protein A-Purified Polyclonal Antibody Package (Rabbit)
* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. y
For antibody purification from pooled serum, 1 ml of pre-immune serum is provided. S Includes Delivery Time Frame ervice • Conjugation to KLH (optional) • 1–2 ml of pre-immune serum (lyophilized)* • Conjugation: 1 week • Two rabbits, pre-immune bleed, • ELISA results • Immunization: 10 weeks s Custom Antibod Custom Polyclonal Antibody Services (Rabbit) four immunizations/rabbit • 200–500 mg of protein A-purified • Purification and ELISA: 1 week • Final bleed antibodies (lyophilized) • Lyophilization and Shipping: 1–2 weeks • Protein A purification • Remaining antigen • Total 13–14 weeks SC1030: Basic Polyclonal Antibody Package (Rabbit) • ELISA test • Lyophilization Includes Delivery Time Frame
• Conjugation to KLH (optional) • 2 ml of pre-immune serum (lyophilized) • Conjugation: 1 week Antigen provided by the customer: 2.5–3 mg of peptide or 2.5–3 mg of protein is required. The concentration should be > 0.4 mg/ml, and no organic • Two rabbits, pre-immune bleed, • ELISA results • Immunization and ELISA: 10 weeks solvents in the antigen solution. four immunizations/rabbit • 100–120 ml of antisera (lyophilized) • Lyophilization and Shipping: • Final bleed from two rabbits 1–2 weeks • ELISA test • Total 12–13 weeks SC1015: Complete Peptide Polyclonal Antibody Package (Rabbit) • Lyophilization Includes Delivery Time Frame Antigen provided by the customer: 2.5–3 mg of peptide or 2.5–3 mg of protein is required. The concentration should be > 0.4 mg/ml, and the antigen solution must not contain organic solvents. • Peptide synthesis (< 15 AA, • 2 ml of pre-immune serum (lyophilized) • Peptide Synthesis: 2–3 weeks > 85% purity, 10 mg) • ELISA results • Conjugation: 1 week • A cysteine is automatically added to • 100–120 ml of antisera (lyophilized) • Immunization and ELISA: 10 weeks the C terminus (or N terminus) of the from two rabbits • Lyophilization and Shipping: 1–2 weeks peptide and conjugated to KLH. • 5mg of unconjugated peptide, MS and • Total 14–16 weeks • Two rabbits, pre-immune bleed, four HPLC reports immunizations/rabbit • Final bleed • ELISA test • Lyophilization
- 59 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 60 - SC1045: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Rabbit) Custom Polyclonal Antibody Services (Goat) Bio-Reagent Center
Includes Delivery Time Frame SC1052: Basic Polyclonal Antibody Package (Goat) • Peptide synthesis (< 15 AA, • 1–2 ml of pre-immune serum (lyophilized)* • Peptide Synthesis: 2–3 weeks > 85% purity, 10 mg) • ELISA results • Conjugation: 1 week Includes Delivery Time Frame • A cysteine is automatically added to • 200–500 mg protein A-purified antibodies • Immunization: 10 weeks • Conjugation to KLH (optional) • 4 ml of pre-immune sera (lyophilized) • Conjugation: 1 week the C terminus (or N terminus) of the (lyophilized) • Purification and ELISA: 1 week • Two goats, pre-immune bleed, • ELISA results • Immunization and ELISA: 10 weeks peptide and conjugated to KLH. • 5 mg of unconjugated peptide, MS and • Lyophilization and Shipping: 1–2 weeks four immunizations/goat • 1,100–1,200 ml of antisera from two goats • Lyophilization and Shipping: 1–2 weeks • Two rabbits, pre-immune, four HPLC reports • Total 15–17 weeks • Final bleed • Total 12–13 weeks immunizations/rabbit • ELISA test • Final bleed • Lyophilization • Protein A purification • ELISA test Antigen provided by the customer: 5 mg of peptide or protein is required. The concentration should be > 0.4 mg/ml, and the buffer must not contain organic • Lyophilization solvents. Bio-Reagent Center
SC1367: Basic Protein G Purified Polyclonal Antibody Package (Goat) SC1031: Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit)
Includes Delivery Time Frame Includes Delivery Time Frame • Conjugation to KLH (optional) • 4 ml of pre-immune sera (lyophilized) • Conjugation: 1 week • Peptide synthesis (< 15 AA, • 1–2 ml of pre-immune serum (lyophilized)* • Peptide Synthesis: 2–3 weeks • Two goats, pre-immune bleed, • ELISA results • Immunization: 10 weeks > 85% purity, 15 mg) • ELISA results • Conjugation: 1 week four immunizations/goat • 300–1,000 mg protein G-purified antibody • Purification and ELISA: 1 week • A cysteine is automatically added to • 3–5 mg affinity-purified antibodies • Immunization: 10 weeks • Final bleed (lyophilized) from 100 ml antisera • Lyophilization and Shipping: 1–2 weeks the C terminus (or N terminus) of the (lyophilized) • Purification and ESLIA: 1 week • Protein G purification of • ≈1,000 ml antisera • Total 13–14 weeks peptide and conjugated to KLH • 5 mg of unconjugated peptide, MS and • Lyophilization and Shipping: 1–2 weeks 100 ml antisera • Two rabbits, pre-immune bleed, four HPLC reports • Total 15–17 weeks • ELISA test immunizations/rabbit • Lyophilization • Final bleed C s • Peptide (5 mg) conjugated to affinity resin ustom Antibod Antigen provided by the customer: 5 mg of peptide or protein is required. The concentration should be > 0.4 mg/ml, and the buffer must not contain organic • Affinity purified antibodies solvents. • ELISA test
Service • Lyophilization y
SC1050: Complete Peptide Polyclonal Antibody Package (Goat) y
S
SC1044: Phospho-Specific Polyclonal Antibody Package (Rabbit) Includes Delivery Time Frame ervice • Peptide synthesis (< 15 AA, > 85% • 4 ml of pre-immune sera (lyophilized) • Peptide Synthesis: 2–3 weeks Includes Delivery Time Frame purity, 10 mg) • ELISA results • Conjugation: 1 week s Custom Antibod • Synthesis of two peptides, one with • 1–2 ml of pre-immune serum (lyophilized)* • Peptide Synthesis: 2–4 weeks • A cysteine is automatically added to • 1,100–1,200 ml of antisera (lyophilized) • Immunization and ELISA: 10 weeks modification (10–15 AA, > 90% purity, • ELISA results • Conjugation: 1 week the C terminus (or N terminus) of th from two goats • Lyophilization and Shipping: 1–2 weeks 15 mg) and the other without • 1–3 mg modified-specific antibodies • Immunization: 10 weeks peptide and conjugated to KLH. • 5 mg of unconjugated peptide, MS and • Total 14–16 weeks modification (10–15 AA, > 90% purity, (lyophilized) from two rabbits • Purification and ELISA: 1 week • Two goats, pre-immune bleed, HPLC reports 10 mg) • 5 mg of modified peptide and 5 mg of • Lyophilization and Shipping: 1–2 weeks four immunizations/goat • A cysteine is automatically added to unmodified-peptide, MS and HPLC reports • Total 15–18 weeks • Final bleed the C terminus (or N terminus) of the • ELISA test peptide and conjugated to KLH. • Lyophilization • Two rabbits, pre-immune bleed, four immunizations/rabbit • Final bleed • Prepare two affinity columns • Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption. • ELISA test • Lyophilization
* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided.
- 61 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 62 - SC1051: Complete Protein G-Purified Peptide Polyclonal Antibody Package (Goat) SC1046: Complete Peptide Polyclonal Antibody Package (Mouse) Bio-Reagent Center
Includes Delivery Time Frame Includes Delivery Time Frame • Peptide synthesis (< 15 AA, > 85% • 4 ml of pre-immune sera (lyophilized) • Peptide Synthesis: 2–3 weeks • Peptide synthesis (< 15 AA, > 85% • 50–100 μl of pre-immune serum (lyophilized) • Peptide Synthesis: 2–3 weeks purity, 10 mg) • ELISA results • Conjugation: 1 week purity, 10 mg) • ELISA results • Conjugation: 1 week • A cysteine is automatically added to • 300–1,000 mg protein G-purified antibody • Immunization: 10 weeks • A cysteine is automatically added to • 2.5 ml of antisera (lyophilized) from all mice • Immunization and ELISA: 10 weeks the C terminus (or N terminus) of the (lyophilized) from 100 ml antisera • Purification and ELISA: 1 week the C terminus (or N terminus) of the • 5 mg of unconjugated peptide, MS and • Lyophilization and Shipping: 1–2 weeks peptide and conjugated to KLH. • ≈1,000 ml antisera • Lyophilization and Shipping: 1–2 weeks peptide and conjugated to KLH. HPLC report • Total 14–16 weeks • Two goats, pre-immune bleed, • 5 mg of unconjugated peptide, MS and • Total 15–17 weeks • Five BALB/c mice, pre-immune bleed, four immunizations/goat HPLC reports four immunizations/mouse • Final bleed • Final bleed • Protein G purification of 100 ml antisera • ELISA test • ELISA test • Lyophilization • Lyophilization Bio-Reagent Center
SC1049: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse)
Custom Polyclonal Antibody Services (Mouse) Includes Delivery Time Frame • Peptide synthesis (< 15 AA, > 85% • 50–100 μl of pre-immune serum (lyophilized) • Peptide Synthesis: 2–3 weeks SC1047: Basic Polyclonal Antibody Package (Mouse) purity, 10 mg) • ELISA results • Conjugation: 1 week • A cysteine is automatically added to • 2.5–12.5 mg purified antibody (lyophilized) • Immunization: 10 weeks Includes Delivery Time Frame the C terminus (or N terminus) of the from all antisera • Purification and ELISA: 1 week • Conjugation to KLH (optional) • 50–100 μl of pre-immune serum (lyophilized) • Conjugation: 1 week peptide and conjugated to KLH. • 5 mg of unconjugated peptide, MS and • Lyophilization and Shipping: 1–2 weeks • Five BALB/c mice, pre-immune bleed, • ELISA results • Immunization and ELISA: 10 weeks • Five BALB/c mice, pre-immune, HPLC report • Total 15–17 weeks four immunizations/mouse • 2.5 ml of antisera (lyophilized) from all mice • Lyophilization and Shipping: 1–2 weeks four immunizations/mouse • Final bleed • Total 12–13 weeks • Final bleed • ELISA test • Antibody purification by protein A • Lyophilization column C s • ELISA test ustom Antibod Antigen provided by the customer: 2.5–3 mg of peptide or 1.5 mg protein (> 85% purity, 90% preferred) is required. The concentration should be > 0.4 • Lyophilization mg/ml, and the antigen solution must not contain organic solvents. Service y
SC1250: Basic Protein A-Purified Polyclonal Antibody Package (Mouse) Custom Polyclonal Antibody Services (Rat) y
SC1311: Basic Polyclonal Antibody Package (Rat) S Includes Delivery Time Frame ervice • Conjugation to KLH (optional) • 50–100 μl of pre-immune serum (lyophilized) • Conjugation: 1 week Includes Delivery Time Frame • Five BALB/c mice, pre-immune bleed, • ELISA results • Immunization and ELISA: 10 weeks s Custom Antibod • Conjugation to KLH (optional) • 50–100 μl of pre-immune serum (lyophilized) • Conjugation: 1 week four immunizations/mouse • 2.5 ml of antisera (lyophilized) from all mice • Lyophilization and Shipping: 1–2 weeks • Three rats, pre-immune bleed, • ELISA results • Immunization and ELISA: 10 weeks • Final bleed • Total 12–13 weeks four immunizations/rat • 5 ml of antisera (lyophilized) from all rats • Lyophilization and Shipping: 1–2 weeks • Protein A purification • Final bleed • Total 12–13 weeks • ELISA test • ELISA test • Lyophilization • Lyophilization
Antigen provided by the customer: 2.5–3 mg of peptide or 1.5 mg protein (> 85% purity, 90% preferred) is required. The concentration should be > 0.4 Antigen provided by the customer: 2.5–3 mg of peptide or protein is required. The concentration should be > 0.4 mg/ml and the antigen solution must not mg/ml, and no organic solvents in the antigen solution. contain organic solvents.
- 63 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 64 - SC1312: Basic Protein G-Purified Polyclonal Antibody Package (Rat) SC1370: Basic Affinity-Purified Polyclonal Antibody Package (Chicken) Bio-Reagent Center
Includes Delivery Time Frame Includes Delivery Time Frame • Conjugation to KLH (optional) • 50–100 μl of pre-immune serum (lyophilized) • Conjugation: 1 week • Conjugation to KLH (optional) • 1–2 ml of pre-immune serum (lyophilized)* • Conjugation: 1 week • Two chickens, pre-immune bleed, • 4 ml of antisera (lyophilized) • Immunization: 10–11 weeks • Three rats, pre-immune bleed, • ELISA results • Immunization: 10 weeks four immunizations/chicken • ELISA results • Purification and ELISA: 2 week four immunizations/rat • 4.5–22.5 mg protein G-purified antibodies • Protein G purification and ELISA: 1 week • Final bleed • Affinity-purified IgY antibodies (lyophilized) • Lyophilization and Shipping: 1–2 weeks • Final bleed (lyophilized) • Lyophilization and Shipping: 1–2 weeks • Antibody purification by peptide affinity • Total 14–16 weeks • Protein G purification • Remaining antigen • Total 13–14 weeks column from yolk • ELISA test • ELISA test • Lyophilization • Lyophilization
Antigen provided by the customer: 2.5–3 mg of peptide or 2.5–3 mg of protein is required. The concentration should be > 0.4 mg/ml, and no organic Antigen provided by the customer: 2 mg of peptide or protein is required. The concentration should be > 0.4 mg/ml and the antigen solution must not solvents in the antigen solution. contain organic solvents. Another 5 mg of antigen is required for purification. Bio-Reagent Center
SC1313: Complete Peptide Polyclonal Antibody Package (Rat) SC1055: Complete Peptide Polyclonal IgY Antibody Package (Chicken)
Includes Delivery Time Frame Includes Delivery Time Frame • Peptide synthesis (< 15 AA, > 85% • 1–2 ml of pre-immune serum (lyophilized)* • Peptide Synthesis: 2–4 weeks • Peptide synthesis (< 15 AA, > 85% • 50–100 μl of pre-immune serum (lyophilized) • Peptide synthesis: 2–3 weeks purity, 10 mg) • 4 ml of antisera (lyophilized) • Conjugation: 1 week purity, 10 mg) • ELISA results • Conjugation: 1 week • A cysteine is automatically added to • ELISA results • Immunization: 10 weeks • A cysteine is automatically added to • 5 ml of antisera (lyophilized) from all rats • Immunization and ELISA: 10 weeks the C terminus (or N terminus) of the • Purified IgY antibodies (lyophilized) • Purification and ELISA: 1 week the C terminus (or N terminus) of the • 5 mg of unconjugated peptide, MS and • Lyophilization and Shipping: 1–2 weeks peptide and conjugated to KLH. • 5 mg of unconjugated peptide, MS and • Lyophilization and Shipping: 1–2 weeks peptide and conjugated to KLH. HPLC report • Total 14–16 weeks • Two chickens, pre-immune bleed, four HPLC reports • Total 15–18 weeks • Three rats, pre-immune bleed, four immunizations/chicken immunizations/rat • Final bleed • Final bleed • IgY Antibody purification • ELISA test • ELISA test • Lyophilization
• Lyophilization C s ustom Antibod
SC1056: Complete Affinity-Purified Peptide Polyclonal Antibody Package (Chicken) SC1314: Complete Protein G-Purified Peptide Polyclonal Antibody Package (Rat) Service y Includes Delivery Time Frame Includes Delivery Time Frame • Peptide synthesis (< 15 AA, > 85% • 1–2 ml of pre-immune serum (lyophilized)* • Peptide Synthesis: 2–4 weeks
• Peptide synthesis (< 15 AA, > 85% • 50–100 μl of pre-immune serum (lyophilized) • Peptide synthesis: 2–3 weeks purity, 15 mg) • 4 ml of antisera (lyophilized) • Conjugation: 1 week y
purity, 10 mg) • ELISA results • Conjugation: 1 week • A cysteine is automatically added to • ELISA results • Immunization: 10 weeks S ervice • A cysteine is automatically added to • 4.5–22.5 mg purified antibody • Immunization: 10 weeks the C terminus (or N terminus) of the • Affinity-purified IgY antibodies (lyophilized) • Purification and ELISA: 2 week the C terminus (or N terminus) of the (lyophilized) from all antisera • Purification and ELISA: 1 week peptide and conjugated to KLH. • 5 mg of unconjugated peptide, MS and • Lyophilization and Shipping: 1–2 weeks
• Two chickens, pre-immune bleed, four HPLC reports • Total 16–19 weeks s
Custom Antibod peptide and conjugated to KLH. • 5 mg of unconjugated peptide, MS and • Lyophilization and shipping: 1–2 weeks immunizations/chicken • Three rats, pre-immune, four HPLC report • Total 15–17 weeks • Final bleed immunizations/rat • Antibody purification by peptide affinity • Final bleed column from yolk • Antibody purification by protein G column • ELISA test • ELISA test • Lyophilization • Lyophilization * For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided. Custom Polyclonal Antibody Services (Chicken)
SC1369: Basic Polyclonal IgY Antibody Package (Chicken)
Includes Delivery Time Frame • Conjugation to KLH (optional) • 1–2 ml of pre-immune serum (lyophilized)* • Conjugation: 1 week • Two chickens, pre-immune bleed, • 4 ml of antisera (lyophilized) • Immunization: 10–11 weeks four immunizations/chicken • ELISA results • Purification and ELISA: 1 week • Final bleed • Purified IgY antibodies (lyophilized) • Lyophilization and Shipping: 1–2 weeks • IgY Antibody purification • Total 13–15 weeks • ELISA test • Lyophilization
Antigen provided by the customer: 2 mg of peptide or protein is required. The concentration should be > 0.4 mg/ml and the antigen solution must not contain organic solvents.
- 65 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 66 - Western-GUARANTEED Antibody Service Custom Monoclonal Antibody Services
(http://www.genscript.com/Guaranteed_Antibody.html) (http://www.genscript.com/monoclonal_antibody_service_detail.html) Bio-Reagent Center
GenScript has a unique antibody production technology. We Our Western guarantee is possible because of proprietary GenScript's monoclonal antibodies are produced in hybridoma is AAALAC International accredited. intend to use this new service package–Western-GUARANTEED OptimumAntigenTM design tool, nanotechnology, and proprietary cell lines derived from the fusion of myeloma cells and peptide polyclonal antibody package to satisfy your need for T-MaxTM adjuvant. Full antibody production documentation antibody-producing B-lymphocytes. GenScript's innovations We guarantee two positive clones for monoclonal antibodies quality antibodies that generate positive Western blot results. including quality assurance comes with the delivery. include advanced OptimumAntigenTM antigen design tool, developed in any chosen host if peptide antigens were Our confidence is backed up by our track record of having GenScript's experienced and knowledgeable staff is there to proprietary T-MaxTM adjuvant, and nanotechnology technology designed, synthesized, and conjugated by GenScript. For any produced thousands of successful custom polyclonal antibodies help you with the antibody you need for your research. for antigen preparation. In addition, our state-of-the-art protein antigen (at least 2 mg, > 50% purity) we guarantee two with a success rate over 98%. hybridoma technology ensures the high quality and competitive positive clones with positive Western blot results for any host, or pricing of our custom monoclonal antibodies. Our animal facility client pays only $800.00 set up fee.
SC1390: Western-GUARANTEED Peptide Polyclonal Antibody Package (Rabbit) Bio-Reagent Center Includes Delivery Time Frame Monoclonal Antibody Services • Peptide design and synthesis > 85% • 1–2 ml of pre-immune serum (lyophilized)* • Total 16–18 weeks
purity, 15 mg • 3–5 mg purified antibodies (lyophilized)
GLNTRKKLQ O
|| R2 Antibody + C H N | 3 C cys • Peptide-KLH conjugation from 2 best rabbits CH NH KLH C | || O R1 • 6 rabbits immunization, pre-immune • 5 mg peptide bleeds and test bleeds • MS and HPLC reports Antigen design Peptide synthesis Animal Antiserum Antiserum Purification QC by Delivery of and coupling immunization production preparation Western blot / ELISA antibody product • Affinity purification and ELISA results • Western blot, ELISA titer reports • Western blot test • Lyophilization
* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided. Custom Monoclonal Antibody Services (Mouse/Rat): Note: All antisera or purified antibodies are provided as lyophilized form except special requirements. Lyophilization extends the shelf life of your antibodies,
Specific services C eliminates leaking problems, loosens up stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped. s ustom Antibod
Cat. No. Service Items SC1301 Phospho-Specific Monoclonal Antibody Package (Mouse) Service
y Quotations and Ordering: SC1398 Phospho-Specific Monoclonal Antibody Package (Rat) SC1184 Anti-idiotype Monoclonal Antibody Package (Mouse)
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the SC1185 Anti-idiotype Monoclonal Antibody Package (Rat) y
most convenient ordering method. For sample submission if applicable, please send to Antibody Services, GenScript, 120 Centennial S Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. ervice Services Specifications Deliverables Timeline s
Custom Antibod • Conjugation to carrier protein • Immunization and ELISA Quotation Form: • Five BALB/c mice (three rats) Obtainable on our website screening: 8–10 weeks SC1040: Basic Monoclonal immunization • Two vials of cell culture for • Fusion and screening: 4–8 Antibody Package (Mouse) • ELISA screening each subclone Email: [email protected] weeks SC1391: Basic Monoclonal • Splenocyte fusions • 5 ml of culture supernatants • Subcloning and delivery: 4–6 Antibody Package (Rat) • Expansion and screening • Parental clones (optional) Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 weeks • Subclone up to five parental • Total Time: 4–6 months Fax: 1-732-210-0262; 1-732-885-5878 clones • Peptide synthesis • Peptide synthesis: 3–4 • Conjugation to carrier protein weeks Secured Web Server: https://www.genscript.com/ssl-bin/secure_data SC1041: Complete Peptide • Five BALB/c mice (three rats) • 5 mg of free peptide • Immunization and ELISA Monoclonal Antibody Package immunization • Two vials of cell culture for screening: 8–10 weeks (Mouse) • ELISA screening each subclone • Fusion and screening: 4–8 SC1392: Complete Peptide • Splenocyte fusions • 5 ml of culture supernatants weeks Monoclonal Antibody Package • Expansion and screening • Parental clones (optional) • Subcloning and delivery: 4–6 (Rat) • Subclone up to five parental weeks clones • Total Time: 5–7 months
- 67 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 68 - Monoclonal Antibody Production Service
(http://www.genscript.com/monoclonal_antibody_production_service.html) Bio-Reagent Center Services Specifications Deliverables Timeline • Peptide synthesis • Peptide synthesis: 3–4 weeks Monoclonal antibodies are extremely useful in both the specific • Unlimited Production: GenScript can produce and purify • Conjugation to carrier protein • Immunization and ELISA screening: SC1301: Phospho-Specific • 5 mg of free modified peptide detection and purification of a variety of substances and in antibodies from any cell line from any species. • 10 BALB/c mice (6 rats) 8–10 weeks Monoclonal Antibody Package • 5 mg of unmodified peptide targeted drug discovery. It is estimated that the advances in • Scalability: Our monoclonal antibody production capability immunization • Fusion and screening: 4–8 weeks (Mouse) • Two vials of frozen cells biopharmaceutical technology and increases in demand will ranges from milligram to gram quantities. • ELISA screening • Subcloning and delivery: 4–6 weeks SC1398: Phospho-Specific • 5 ml of culture supernatants bring the market for monoclonal antibodies to over 30 billion • Competitive Prices: Our guaranteed monoclonal antibody • Splenocyte fusions • Total Time: 5–7 months Monoclonal Antibody Package • Parental clones (optional) USD by 2010. service costs as little as many other companies' polyclonal • Expansion and screening (Rat) While high-affinity monoclonal antibodies make an irreplaceable services. • Subclone up to five positive contribution to many different kinds of research, large-quantity • Strict Confidentiality: The customer wholly owns the parental clones production of these antibodies can be a roadblock. GenScript's information, cell lines, and antibodies generated from his/her tailored facilities and experienced staff offer specialized project. If necessary, GenScript will sign non-disclosure monoclonal antibody production, purification, and modification documents.
Bio-Reagent Center services, sidestepping the slowing effects of monoclonal Customized Monoclonal Antibody Services: antibody product in the pharmaceutical process. GenScript Ascites Production Services offers ascites in vivo and roller bottle in vitro production services as well as test run services for large-scale production. Our GenScript offers a variety of monoclonal antibody production you toward the best selection for your specific needs. Our GenScript ascites production is performed in BALB/c or animal facility is AAALAC International accredited. packages. Our experienced sales account managers will guide monoclonal antibody production service starts at $3,800.00. immunodeficient mice. Our typical deliverables include 2–5 ml ascites per mouse at antibody concentration of 1–10 mg/ml Services: depending on the productivity of individual cell line. Our typical Key Features: turnaround time is 4–6 weeks. Assemble your own packages: • Guaranteed Delivery: We guarantee two ELISA-positive GenScript also offers mycoplasma testing, mycoplasma clones with any antigen for a complete hybridoma removal, and clonality services for premium ascites production. Cat. No. Specifications development project. Monoclonal Antibody Project, Phase I, Immunization of 10 mice, test bleed and ELISA, up to 10 months of maintenance SC1384 • Handling Problematic Cell Lines: GenScript can produce Monoclonal Antibody Project, Phase I, cell culture and immunogen preparation, immunization of 10 mice, test bleed and SC1385 ascites from solid-tumor-producing cell lines. ELISA, up to 10 month of animal maintenance C s Monoclonal Antibody Project, Phase I, immunization of up to 5 BALB/c mice with protein or carrier protein conjugated ustom Antibod SC1216 haptens, test bleed, ELISA, and evaluation SC1217 Monoclonal Antibody Project, Phase I, immunization of up to 3 rats with protein or carrier protein conjugated haptens Ascites Production in BALB/c Mice
Service SC1219 Monoclonal Antibody Project, Phase II, cell fusions, screening, positive clone expansion, and isotyping y SC1220 Monoclonal Antibody Project, Phase III, subcloning, screening and expansion SC1221 Additional Subcloning (in Phase II) Cat. No. No. of Mice Price
SC1110 5 $350.00 y SC1223 Monoclonal Antibody Production, Antigen Screening and Testing S
SC1111 10 $525.00 ervice SC1112 20 $630.00 SC1113 50 $1,125.00 s
Custom Antibod SC1114 100 $1,880.00 Quotations and Ordering: SC1115 200 $3,030.00 SC1116 > 300 Upon Request Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For sample submission if applicable, please send to Antibody Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. Ascites Production in Immunodeficient Mice (Nude/Scid)
Cat. No. No. of Mice Price Quotation Form: Obtainable on our website SC1302 5 $525.00 SC1303 10 $787.50 Email: [email protected] SC1304 20 $945.00 SC1305 50 $1,687.50 Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 SC1306 100 $2,820.00 SC1307 200 $4,545.00 Fax: 1-732-210-0262; 1-732-885-5878 SC1308 > 300 Upon Request
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
- 69 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 -70 - Express Antibody Services
Roller Bottle Cell Culture Services (http://www.genscript.com/Express_Antibody.html) Bio-Reagent Center
Roller bottle cell culture allows for high-quality, reproducible deliverables include 15–50 mg/L monoclonal antibodies GenScript's proprietary protocol delivers superior quality custom Key Features: antibody production without the use of animals. Typically, either depending on the productivity of individual cell line. Our typical polyclonal antibody service in 33 days and hybridomas low-bovine-IgG fetal bovine sera or serum-free media are used turnaround time is 5–7 weeks. We offer mycoplasma testing, development in 60 days. These two service protocols deliver • Fast Turnaround Time: Just 8 weeks from immunization to in roller bottle cell culture to control the possible bovine IgG mycoplasma removal, and clonality services for optimized antibodies in a timely fashion and aim to satisfy your urgent hybridoma cell production. contamination. Typically, monoclonal antibodies produced in production. antibody needs. This is achieved through a smooth integration • Superior Quality: We guarantee two ELISA positive clones roller bottles are purified before shipment. Our typical of GenScript’s proprietary OptimumAntigenTM design tool, for antigens designed and produced by GenScript, and any nanotechnology, and proprietary T-MaxTM adjuvant. Our animal other qualified immunogens. facility is AAALAC International accredited. • Advanced Technologies: Proprietary OptimumAntigenTM Roller Bottle Cell Culture design tool, nanotechnology, and proprietary T-MaxTM MonoExpressTM Antibody Services adjuvant. Cat. No. No. of Mice Price • High Capability: Customize antibody production for difficult
Bio-Reagent Center SC1117 1 $315.00 This service protocol produces monoclonal antibody with quality antigens. SC1118 2–5 $295.00 meeting your less demanding needs. The express service SC1119 6–10 $260.00 delivers monoclonal antibody in only 60 days. SC1120 11–20 $245.00 SC1121 21–50 $230.00 SC1122 > 50 Upon Request Service Packages and Timelines
Low Endotoxin Unit (EU) Roller Bottle Cell Culture Services Specifications Deliverables • 10 BALB/c mice (6 rats) immunization, pre-immune • Cryopreservation and delivery of up SC1342: Basic MonoExpressTM bleeds and test bleeds to 5 positive hybridoma clones and Cat. No. No. of Mice Price Antibody Service (Mouse) • Cell fusion, cloning and ELISA screening 3–5 ml of culture supernatant for SC1373 1 $345.00 SC1343: Basic MonoExpressTM • ELISA/Western blot test each clone. SC1374 2–5 $325.00 Antibody Service (Rat) Total time: 8–10 weeks SC1375 6–10 $290.00 • Frozen positive cell lines • Custom Antibod
s • Peptide synthesis • Cryopreservation and delivery of up SC1376 11–20 $270.00 SC1344: Peptide Supplied • Peptide-KLH conjugation to 5 positive hybridoma clones and SC1377 21–50 $255.00 MonoExpressTM Antibody • 10 BALB/c mice (6 rats) immunization, pre-immune 3–5 ml of culture supernatant for SC1378 > 50 Upon Request Service (Mouse) bleeds and test bleeds each clone. Service
y SC1345: Peptide Supplied • Cell fusion, cloning and ELISA screening • 3 mg unconjugated peptide MonoExpressTM Antibody • ELISA/Western blot test • MS and HPLC reports Service (Rat) • Frozen positive cell lines • Total time: 10–12 weeks y Service Quotations and Ordering: Note: Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose 1. If the project is terminated at any time during Immunization, the client needs only pay $1,500.00 plus the cost of any additional services ordered that has s
Custom Antibod the most convenient ordering method. For sample submission if applicable, please send to Antibody Services, GenScript, 120 been performed. Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. 2. To start any project, the customer should pay $1,500 startup fee plus the cost of services to be performed during Immunization. The rest of the bill should be paid upon the final delivery.
Quotation Form: Obtainable on our website
Email: [email protected] Express Rabbit Polyclonal Antibody Services Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 Key Features: The lengthy and complex procedures involved in antibody Fax: 1-732-210-0262; 1-732-885-5878 production increase the cost, duration, and uncertainty of R&D • Fast Protocol：Only 33 days needed from immunization to projects. Using OptimumAntigenTM design tool, antisera extraction. Secured Web Server: https://www.genscript.com/ssl-bin/secure_data nanotechnology, and our proprietary T-MaxTM adjuvant, • Guaranteed final ELISA titer of 1:32,000 and positive GenScript provides both confidence and speed in polyclonal Western blot results for any protein antigen (3–5 mg, > 85% antibody production. purity, 90% preferred)
- 71 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 72 - Immunochemistry Services
(http://www.genscript.com/Immunochemistry_Services.html)
Service Packages and Timelines Bio-Reagent Center
Services Specifications Deliverables Timeline GenScript Immunochemistry Services focus on antibody stage of its antibody service package. These range from • Antigen submission: purification, modification, and other related services. relatively common methods, such as Protein A and Protein G, to o 5–6 mg peptide or protein Protein L. Our Antibody Purification Services offer GenScript’s o 0.4 mg/ml or higher Antibody Purification Services clients a solution package that can purify any antibody quickly • 2 ml of pre-immune sera o No organic solvents • Conjugation: 3 days and efficiently. SC1178: Basic Express (lyophilized) • Conjugated to KLH (optional) • Immunization: 33–36 days GenScript provides a wide array of options at the purification Polyclonal Antibody Package • Four rabbits, pre-immune bleed • ELISA results • ELISA and shipping: 7 days (Rabbit) • Three immunizations/rabbit • 80 ml of antisera (lyophilized) • Total 43–46 days Cat. No. Service Items • Final bleed from two rabbits SC1043 Protein A/G Affinity Purification (up to 100 ml of sera or ascites) • ELISA test SC1127 Protein A/G Affinity Purification (100–1,000 ml of sera or ascites) • Lyophilization
Bio-Reagent Center SC1227 Protein A/G Affinity Purification (up to 2.0 liters of culture supernatant) • Peptide synthesis: SC1228 Protein A/G Affinity Purification (> 2.0 liters of culture supernatant) o < 15 mer SC1039 Peptide Affinity Column Purification (up to 100 ml of sera or ascites) o 20 mg SC1123 Peptide Affinity Column Purification (100–1,000 ml of sera or ascites) o > 85% purity • 2 ml of pre–immune sera • Peptide synthesis: 14 days SC1365 Ammonium Sulfate Precipitation Egg Yolks (up to 10 yolks) • Cysteine addition (C-terminal or (lyophilized) • Conjugation: 3 days SC1179: Complete Express SC1366 Antigen Affinity Purification for Chicken IgY (up to 10 yolks) N-terminal) • ELISA results • Immunization: 33–36 days Peptide Polyclonal Antibody SC1131 Protein L Affinity Purification (up to 100 ml of sera or ascites) • Conjugation to KLH (optional) • 80 ml of antisera (lyophilized) • ELISA and shipping: 7 days Package (Rabbit) SC1132 Protein L Affinity Purification (100–1,000 ml of sera or ascites) • Four rabbits, pre-immune bleed from two rabbits • Total 57–60 days SC1213 Protein-based Immuno-affinity Purification (up to 100 ml of antisera) • Three immunizations/rabbit • 5 mg of unconjugated peptide SC1341 GST/His/Trx/Flag Affinity Column Cross Absorption • Final bleed • MS and HPLC reports SC1124 Ion Exchange Purification (up to 100 ml of sera or ascites) • ELISA test SC1125 Ion Exchange Purification (100–1,000 ml of sera or ascites) • Lyophilization SC1128 Low EU (Endotoxin Unit) Protein A/G Purification (up to 100 ml of sera or ascites) • Peptide synthesis: SC1129 Low EU Protein A/G Purification (100–1,000 ml of sera or ascites) o < 15 mer SC1381 Low EU Protein A/G Affinity Purification (up to 2.0 liter of culture supernatant) C
s o 20 mg SC1382 Low EU Protein A/G Affinity Purification (> 2.0 liter culture supernatant) ustom Antibod o > 85% purity SC1126 Low EU Ion Exchange Purification (up to 100 ml of sera or ascites) • Cysteine addition (C-terminal or • 2 ml of pre-immune sera • Peptide synthesis: 14 days SC1130 Low EU Ion Exchange Purification (100–1,000 ml sera or ascites) SC1180: Complete Express N-terminal) (lyophilized) • Conjugation: 3 days Service SC1133 Low EU Protein L Purification (up to 100 ml of sera or ascites) y Affinity-Purified Peptide • Conjugation to KLH • ELISA results • Immunization: 33–36 days SC1134 Low EU Protein L Purification (100–1,000 ml of sera or ascites) Polyclonal Antibody Package • Four rabbits, pre-immune bleed • 3–5 mg of affinity-purified • Purification: 5 days antibodies (lyophilized) (Rabbit) • Three immunizations/rabbit • ELISA and shipping: 7 days Note: y
• Final bleed • 5 mg unconjugated peptide • Total 62–65 days S For purification of larger quantities of antibody please request a quotation. ervice • 5 mg of peptide conjugated to resin • MS and HPLC reports • Affinity purified antibody s
Custom Antibod • ELISA test Antibody Modification Services • Lyophilization GenScript offers many antibody modification options, including antigen-binding (Fab) portion of the antibody. For these antibody labeling and antibody fragmentation. Labeling applications, antibodies may be enzymatically digested to antibodies with biotin, HRP and various fluorescent probes produce either an Fab or an F(ab')2 fragment of the antibody. Quotations and Ordering: provide signals for visualization and quantitation of the target GenScript's high-quality modification services will give our molecule. In some assays, it is preferable to use only the clients better results in experiments and other downstream Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose applications. the most convenient ordering method. For sample submission if applicable, please send to Antibody Services, GenScript, 120 Services Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form.
Cat. No. Service Items SC1315 Fab Fragmentation (up to 100 mg) Quotation Form: Obtainable on our website SC1060 F(ab')2 Fragmentation (up to 100 mg) SC1061 Biotin Conjugation (up to 20 mg) Email: [email protected] SC1062 HRP Conjugation (up to 20 mg) SC1063 FITC Conjugation (up to 20 mg) Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 SC1348 Texas Red Conjugation (up to 5 mg) SC1349 Other Antibody Conjugate preparation Fax: 1-732-210-0262; 1-732-885-5878 Note: Secured Web Server: https://www.genscript.com/ssl-bin/secure_data For purification of larger quantities of antibody please request a quotation.
- 73 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 74 - Large-scale Antibody Production Services Additional Services (http://www.genscript.com/antibody_large_scale.html) Relevant Antibody Services Bio-Reagent Center Cat. No. Services Price Antibody production is widely needed in biological research SC1020 Peptide Synthesis Starting at $3.20/residue Benefits of Core Technologies: nowadays, although difficulties for making an antibody include SC1032 Peptide Antigen Design $100.00 poor immune response, high background for applications and SC1035 KLH/OVA/BSA Conjugation (up to 20 mg) $150.00 These technologies allow us to offer our customer with: hard-to-obtain antigen. GenScript has developed several novel SC1371 Affinity Resin Shipment (price per custom project) $50.00/per shipping 1) GenScript’s antibody production service guarantees: technologies to overcome the difficulties, allowing us to provide SC1386 ELISA (indirect, price per test for up to 5 testing samples) $35.00 • A final product with an ELISA titer of 1:32,000 or better for Additional Polyclonal Antibody Services high quality and high throughput antibody production services. any host polyclonal antibody project that uses peptide AAALAC International accredited. Cat. No. Services Price Our animal facility is antigens designed, synthesized, and conjugated by SC1036 Antibody Titer Test by ELISA $100.00 GenScript. For any protein antigen (3 mg, > 85% purity, 90% Core Technologies: SC1037 Western Blot Testing (up to 5 antibody samples for one antigen or up to 5 antigens for one antibody) $150.00 preferred), we guarantee a polyclonal antibody with positive SC1196 Pre-screen (hold for up to 10 days after bleed) $19.00/rabbit Western blot results and an ELISA titer of 1:32,000 or better Our proprietary technologies include the followings: SC1350 Rabbit Additional Production Bleed (10–15 ml) and ELISA Test (per animal) every 14 days $45.00 for any host. Or we will restart the project free of charge Bio-Reagent Center 1. OptimumAntigenTM design tool that combines the industry's SC1351 Goat Additional Production Bleed (50–100 ml) and ELISA Test (per animal) $95.00 until the guarantee is met. most advanced algorithms with our time-tested expertise on SC1352 Chicken Additional Production Bleed with ELISA $150.00 • Two ELISA positive clones with any qualified antigen for thousands of antibody production experiments, taking into SC1353 Additional 1 Week Egg Collection and ELISA Test $150.00 monoclonal antibody production. For any protein antigen (at account all available factors for high-quality antibodies SC1355 Mouse Additional Production Bleed with ELISA $165.00 least 2 mg, > 50% purity) we guarantee two positive clones 2. Immuno-modulator that is twice as efficient as Freund's SC1356 Rat Additional Production Bleed with ELISA $165.00 with positive Western blot results for any host, or client pays adjuvant, increasing antibody titer at least 10 folds SC1357 Additional Boost Immunization (rabbit/goat/chicken/mouse/rat) $100.00/injection only $800.00 set up fee. 3. Nanotechnology that processes antigen mixtures down to SC1387 Additional Goat $380.00 2) Large-scale antibody production capability: nanoparticles for maximal immunization efficiency SC1388 Additional Mouse $150.00 • GenScript has delivered more than 5,000 high-quality 4. T-MaxTM adjuvant is significantly more potent in inducing the SC1389 Additional Rat $150.00 custom monoclonal and polyclonal antibodies to our immune reaction than the standard Freund’s adjuvant, SC1358 Animal Maintenance (mouse) $0.5/day customers worldwide. which ensures high specificity and high titer of the produced SC1360 Animal Maintenance (rat) $0.6/day • GenScript has an extensive collection of over 500 antibodies SC1195 Animal Maintenance (rabbit) $1.30/day phospho-specific antibodies covering 13 of the most 5. Diverse species including rabbit, mouse, rat, and goat for SC1361 Animal Maintenance (goat) $1.80/day important signal transduction pathways and an extensive polyclonal antibody production and mouse and rat for SC1362 Animal Maintenance (chicken) $1.40/day panel of tag antibodies including all major tags. monoclonal antibody production nd Custom Antibod s SC1363 Sterile Filtration and Packaging (up to 20 ml) $15.00 • The 2 generation of our anti-His monoclonal antibody is the SC1364 Egg Yolk Separation and Storage ( up to 3 months) $7.00/egg best in the industry. SC1033 Additional Animal for Polyclonal Antibody(rabbit) $250.00/rabbit
Service Extended Protocol for Polyclonal Antibody (per rabbit, additional injection, additional production
y SC1034 $100.00/rabbit bleed with ELISA and animal maintenance) Extended Protocol for Polyclonal Antibody (per goat per month, 1 immunization, 1 production bleed Quotations and Ordering: SC1372 $150.00 (50–100 ml), ELISA test and animal maintenance) y Service SC1322 Antibody Lyophilization, with non-Protein Stabilizer (for up to 10 mg in up to 50 vials) $150.00 Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Extended Monoclonal Antibody Service the most convenient ordering method. For sample submission if applicable, please send to Antibody Services, GenScript, 120 Cat. No. Services Price Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. s
Custom Antibod SC1057 Cell Line Storage (LN2) (6 vials/cell line/year) $60.00 SC1058 Working Cell Bank (50 vials/cell line) $350.00 SC1059 Master Cell Bank (100 vials/cell line) $950.00 Quotation Form: Obtainable on our website SC1379 Cell Culture Supernatant Concentration, 10–20 Times, price per liter $45.00 SC1380 Low EU Cell Culture Supernatant Concentration, 10–20 Times, Price per Liter $75.00 Email: [email protected] SC1383 Additional Cell Fusion, 1–2 Mice or 1 Rat per Fusion $800.00 Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878 Quotations and Ordering:
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method.
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
- 75 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 76- Immunoassay Development Services
(http://www.genscript.com/antibody_assay_development.html) Delivery Specifications: Bio-Reagent Center
The delivery specifications of each service vary depending on and delivery information with each client. In general, we deliver Modern drug discovery, pre-clinical, and clinical studies demand • Comprehensive Services: Our immunoassay development the types of services requested and the assay requirements. cell lines, antibodies and conjugates, assay protocols, assay reliable high-throughput assays for drug compound detections service package spans from immunogen preparation, Since every project is designed-to-order, our scientists and reagents, assay kits, experiment records, and specific data in serum and other biological samples. Because of the antibody production and characterization to immunoassay project managers will discuss project design, cost estimates, derived from a given project. complexity and long time commitments involved in the development and validation. development of such assays, particularly highly specific or • Strong Scientific Support: Each project is carefully evaluated efficient assays and immunoassays, many pharmaceutical and designed by experienced scientists. companies, biotechs, and academic institutions have found that immunoassay development has become a bottleneck that holds Services: Quotations and Ordering: up valuable research. Our immunoassay development services are project-based and Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose GenScript's efficient and reliable antibody production and designed for drug discovery and clinical studies. Our scientists the most convenient ordering method. Bio-Reagent Center immunoassay development services provide a solution to this and project managers will discuss with each client regarding the problem, delivering top-quality assays and freeing up necessary project design, cost estimates, and delivery specifications. We staff and facilities. offer two basic categories of services: Immunoassay Quotation Form: Obtainable on our website Development Service for Drug Discovery and Immunoassay Key Features: Development Service for Therapeutic Antibody Detection. In Email: [email protected] general, our services include the following: • Fast Turnaround: At GenScript, most projects of this kind • Antigen and immunogen preparation Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 take only 7–9 months from immunogen preparation and • Antibody production, purification and modification animal immunization to antibody production and • Cell line generation Fax: 1-732-210-0262; 1-732-885-5878 immunoassay development. This is 25%–40% faster than • Immunoassay development the average industry standard of 12 months. • Reagent preparation • High Efficiency: Our proprietary technologies, combined with • Assay kit manufacturing Secured Web Server: https://www.genscript.com/ssl-bin/secure_data over 25 years of experience, ensure the project's success and the quality of the results. C s ustom Antibod
SC1181: Immunoassay Development Services for Drug Discovery Service y y
Drug Compound S ervice
Compound-carrier Antibody + protein conjugate →→→ →→ s
Custom Antibod KLH/BSA Immunization Polyclonal and/or Antibody purification Immunoassay Assay kit monoclonal conjugation and development and manufacturing antibody production characterization validation
SC1182: Immunoassay Development Services for Therapeutic Antibody Detection
→ → →→ →→Antibody →
Therapeutic Fab Animal Anti-idiotype Antibody Antibody purification Immunoassay Assay kit antibody Fragments immunization monocional screening and conjugation and development and manufacturing antibody production characterization characterization validation
- 77 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 78 - Cell Line Services (http://www.genscript.com/cell_line_services.html) Service Specifications: Bio-Reagent Center
Milestones Specifications Deliverables Timeline With state-of-the-art facilities and seasoned industry Core In-house Technologies: • Amplification/isolation of the gene experience, GenScript offers quality services for generating and Generation of recombinant or synthetic gene of interest into a evaluating primary clones for productivity and adaptability in • 293TGETM transient expression system: Protein can be mammalian cell expression vector Construct(s) report 3 weeks serum-free suspension culture, identifying cell lines for stable, produced ranging from a few milliliters to 100 liters. Our vector construct • Verification of the subcloned long-term production and scalability, and for developing typical results achieve high concentrations of harvestable genes by sequencing large-scale purification and analytical processes. We also and active products. Expression data provide assay-ready frozen cells, preserving your time for • OptimumGeneTM codon optimization: The expression • Conduct transient expression trial Pilot transient expression Transient expression cells 1 weeks high-value projects. enhancement technology has been applied to design tens • SDS-PAGE/WB/ELISA analysis Conditioned media of thousands of genes achieving optimized expression level • Stable transfection and improved protein solubility. Stable cell lines Service List: TM TM • Clone selection and screening • Ready-to-Use frozen cells: The series of Ready-to-Use Stable cell line generation Expression data 7 weeks • Select top three lines,expand
Bio-Reagent Center frozen cells enable assays to be done in 24 hours. QC data • Stable Cell Line Services for Protein and Monoclonal culture, and monitor stability Antibody Production • Optimize various productivity- • Custom Stable Cell Line Services for Assay Development Applications: enhancing conditions • Transient Gene Expression Service Stable cell line • Analyze cell growth rate, subcultivation ratios and cell Stable cell lines • Process Development and Transfer Satisfy your needs in drug discovery: development for Request stability Expression data • Catalog Stable Cell Lines • For small molecule drug discovery: Stable cell lines for large-scale production TM • Expand cell culture,protein • Ready-to-Use Frozen Cells cell-based assay and compound screening QC data purification and process • For large molecule drug discovery: Production of development recombinant proteins and monoclonal antibodies
Pricing:
Cat. No. Service Price Cus s SC1260 Custom Stable Cell Line Generation Quote
Stable Cell Line Services for Protein & Monoclonal Antibody Production t o m
ervice (http://www.genscript.com/stable_cell_line.html) Ce S ll Lin Development of stable cell lines capable of producing fastest results suiting your individual needs. GenScript
Quotations and Ordering: e ell Line
recombinant proteins and monoclonal antibodies at high and scientists will work with you to develop your stable cell line and Se C reliable levels can be critical to a biopharmaceutical research perform functional testing. It is our commitment to ensure that
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose rvi project. GenScript is proud to offer our custom services in the cell line meets all your requirements. tom the most convenient ordering method. For sample submission if applicable, please send to BioProcess Services, GenScript, 120 ces s creating and selecting stable clones for protein production. u Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. C Equipped with state-of-the-art facilities, GenScript's expert staff is ready to carry out complete studies on cell lines having Key Features: genetic and productive stability. We evaluate primary clones for Quotation Form: Obtainable on our website productivity and adaptability for growth in serum-free • A committed team of scientists with years of experience in suspension culture, and identify cell lines with the best potential developing high-productivity stable cell lines Email: [email protected] for stable, long-term production and scalability. • Customize stable cell line development based on customers’ specifications Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 The selected cell lines matching the production • Wide range of functional testing assays requirements can save a significant amount of time during later • Flexibility in parental cells: 293, CHO, BHK, NS0 cells, or Fax: stages of your project. GenScript is capable of delivering gene your specified cell lines 1-732-210-0262; 1-732-885-5878 synthesis, cloning, vector design and construction, protein • State-of-the-art facilities capable of supporting large-scale purification and characterization, as well as assay development protein productions Secured Web Server: https://www.genscript.com/ssl-bin/secure_data services. Our integrated services are at your immediate • Track record of fast on-time delivery fingertips and can be easily leveraged to produce the best and
- 79 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 -80 - Custom Stable Cell Line Services for Assay Development Transient Gene Expression Service
(http://www.genscript.com/stable_cell_line_development.html) (http://www.genscript.com/transient_gene_expression.html) Bio-Reagent Center
Stable cell line development is the key step to develop a Key Features: Transient Gene Expression (TGE) system offers a rapid and impressive success rate resulted from our transient production cell-based assay development. Based on proprietary codon reliable solution to your protein production. One prominent platform has proven to be a reliable tool for rapid productions of optimization and vector design technologies, GenScript has • Any GPCR and ion channel of your interest characteristic of the TGE platform is its ability to generate a recombinant proteins and monoclonal antibodies. developed over 60 GPCR and ion channel stable cell lines with • Stable cell lines for increased reliability and reproducibility large quantity of protein samples. Expression levels of proteins quality and speed. Custom Stable Cell Line Service is also • Flexible Functional readouts are available delivered via these suspension-adapted cells can reach 50–100 Competitive Advantages: available for your target of interest. • Rapid turnaround mg/L even under un-optimized conditions. Using the TGE system, milligrams or even grams of recombinant monoclonal • The proprietary 293 and CHO suspension system antibodies can be generated within three to five weeks. promote fast production and delivery of recombinant GenScript's transient mammalian expression system has been proteins and monoclonal antibodies up to grams level used to produce a diverse range of protein products. The TGE for your drug discovery and bioanalytical assays. Milestones Specification Delivery Timeline system can easily produce proteins with flexible scales from • The serum free cell culture condition makes the • Subclone the synthetic gene of interest several milliliters to 100 liters. We typically achieve high downstream purification processing much easier. Bio-Reagent Center Generation of recombinant vector into the target vector – 3 weeks concentrations of harvestable and active products. The constructs • Verify the subcloned genes by sequencing • Mycoplasma test of host cells Transfection and cell selection • Transfection – 10 –14 days Pricing: • Cell selection Isolation of antibiotics resistant clones Pick 96 positive clones into 96-well plate – 2–3 days Cat. No. Service Price First functional assay 2 conc. against stimulation – 1 day SC1259 Mammalian Cell Protein Expression Services Quote • Pick 6 best clones into 24-well plate
Second functional assay • functional assay: 2 conc. (EC50 and – 3–4 days
Cmax), at n=2 • Mycoplasma test Quotations and Ordering: Subcloning • Pick 1 best clone to second round of – 14 days subcloning Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Cus
s • Pick 15 subclones for assay the most convenient ordering method. For sample submission if applicable, please send to BioProcess Services, GenScript, 120 Third functional assay • Functional assay: 2 conc. (EC and – 1 day
50 t
Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. o
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Stable cell line, EC50 report, Forth functional assay • Pick the best subclone into 6-well plate 7–10 days ll Lin and QC report Quotation Form: Obtainable on our website • EC50 determination
• 16 passages e ell Line
Stability test Stability test report 5 weeks Email: [email protected] Se C • EC50 determination rvi
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Quotations and Ordering:
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For sample submission if applicable, please send to Bio-Assay Center, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form.
Quotation Form: Obtainable on our website
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878
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- 81 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 82 - Process Development and Transfer
(http://www.genscript.com/process_development.html) GPCR Stable Cell Lines Bio-Reagent Center
With in-house experienced scientist team and proprietary and hydrophobic interactions are available. The purification Cat. No. Family Receptor Cat. No. Family Receptor development platform, GenScript delivers custom services for tags can be removed via a specific protease if desired, and the M00185 Acetylcholine (muscarinic) M1 M00198 Lysophospholipid EDG3 growth and production media optimization for target cell lines. protein is further purified by gel filtration. M00135 Acetylcholine (muscarinic) M1 M00197 Lysophospholipid EDG5 We define feeding strategies and study cell line stability to M00259 Acetylcholine (muscarinic) M3 M00151 Lysophospholipid EDG8 increase production scale. We also perform thorough analysis M00186 Acetylcholine (muscarinic) M5 M00211 Melanin-concentrating Hormone MCHR1 on growth rate, subcultivation ratios, cell mass, and other Customized Services: M00225 Adrenoceptors ADRA1A M00127 Melanin-concentrating Hormone MCHR2 parameters. Upon request, we can also carry out additional M00260 Adrenoceptors ADRA1B M00272 Melanocortin MC4 specific process optimization studies and employ our • Flexible Expression Cell Lines: Hybridoma, 293, CHO, BHK, M00269 Adrenoceptors ADRB1 M00191 Neuromedin U NMU1 scaled-down models to predict cell performance. NS0, and more M00182 Bombesin BB2 M00194 Neurotensin NTS1 GenScript also optimizes protein purification and process • Flexible Purification Assays: SDS-PAGE, IEF, HPLC, ELISA, M00183 Bombesin BB3 M00224 Orexin OX1 development. Down-stream protein purification is typically and more M00184 Bradykinin B2 M00195 Oxytocin OT/OXTR conducted using affinity chromatography, other • Flexible Accessory Services: Vector improvement, protein M00271 Cholecystokinin CCKA M00148 Parathyroid Hormone PTH1 Bio-Reagent Center chromatographic methods including gel filtration, ion exchange, characterization, and specific optimization services M00154 Cholecystokinin CCKB M00141 Parathyroid Hormone PTH2 M00208 Corticotropin Releasing Factor CRF2 M00262 Platelet Activating Factor PAF/PTAFR M00128 Dopamine D1 M00147 Platelet Activating Factor PAF/PTAFR M00152 Dopamine D2 M00155 Prostanoid FP M00129 Dopamine D2 M00228 Prostanoid PTGER1 Quotations and Ordering: M00130 Dopamine D5 M00206 Proteinase-activated Receptor PAR4 M00149 Endothelin EDNRA M00251 Serotonin 5-HT2A Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose M00150 Endothelin EDNRB M00249 Serotonin 5-HT2B the most convenient ordering method. M00263 Free Fatty Acid FFA1/GPR40 M00253 Serotonin 5-HT2C M00273 Free Fatty Acid FFA1/GPR40 M00144 Tachykinin NK1/SPR M00137 Free Fatty Acid FFA1/GPR40 M00199 Tachykinin NK1 Email:[email protected] M00277 Galanin GAL1 M00200 Tachykinin NK2 M00209 Galanin GAL2 M00201 Tachykinin NK3 Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 M00189 Ghrelin GHSR M00202 Thyrtropin-releasing Hormone TRH1 Cus
s M00136 Gonadotropin-Releasing Hormone GnRH M00181 Vasopressin V1A
Fax: t
1-732-210-0262; 1-732-885-5878 M00131 Histamine H1 M00205 Vasopressin V1B o m
ervice M00132 Histamine H2 M00170 Vasopressin V2 Ce S Secured Web Server: https://www.genscript.com/ssl-bin/secure_data M00133 Histamine H3 M00187 Host Cell Line N/A
M00134 Histamine H4 M00188 Host Cell Line N/A ll Lin
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u Cat. No. Family Name Cat. No. Family Name C M00233 TRP HEK293/TRPV1 M00235 Purinoceptor HEK293/P2X1 Catalog Stable Cell Lines M00234 TRP HEK293/TRPA1 M00236 Purinoceptor HEK293/P2X3
http://www.genscript.com/cell_lines.html
GenScript offers a wide selection of GPCR and ion channel Key Features: stable cell lines. Proprietary codon optimization and vector design technologies enable GenScript to overcome low • High functional expression of proteins at the cell surface functional expression of protein at the cell surface. All cell lines • Stable cell lines for increased reliability and reproducibility have been optimized for S/N ratio in calcium flux assay or other • All ion channel stable cell lines are validated by manual assays and shown stability after over 16 passages. patch clamp, and Qpatch • Flexible functional readouts are available Tailored to your specifications, GenScript Custom Stable Cell Line Service is available.
-83 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 84 - Ready-to-UseTM Frozen Cells
(http://www.genscript.com/ready-to-use_frozen_cells.html) Product List: Bio-Reagent Center
Cat. No. Name Family Receptor Size Price (USD) Cell-based assays can often be challenging and time- validated with stringent criteria to assure consistent results. M00175 CHO-K1/M5 Cells Ready-to-UseTM Acetylcholine M5 1 vial $399.00 consuming. To smooth and streamline this complicated • Convenience: GenScript’s Ready-to-UseTM Frozen Cells can M00178 CHO-K1/BB2 Cells Ready-to-UseTM Bombesin BB2 1 vial $399.00 process, GenScript has developed a series of Ready-to-UseTM trim time and aggravation out of functional testing. Instead M00164 CHO-K1/BB3 Cells Ready-to-UseTM Bombesin BB3 1 vial $399.00 Frozen Cells that can be conveniently employed in functional of engaging in a lengthy in-house culturing process, simply M00157 CHO-K1/EDNRA Cells Ready-to-UseTM Endothelin EDNRA 1 vial $399.00 GPCR receptor assays. GenScript scientists culture and freeze thaw the cells, plate them, and run the assays. M00158 CHO-K1/EDNRB Cells Ready-to-UseTM Endothelin EDNRA 1 vial $399.00 the cells using optimized protocols that can arrest cell growth to • Variability arrest: Chemically treated cells with optimized M00180 CHO-K1/GHSR Cells Ready-to-UseTM Ghrelin GHSR 1 vial $399.00 reduce variability in cellular assays. protocol to alleviate biological variability. M00162 293/H1 Cells Ready-to-UseTM Histamine H1 1 vial $399.00 • Speed: Assays can be finished within 24 hours after thawing M00159 CHO-K1/EDG8/Gα15 Cells Ready-to-UseTM Lysophospholipid S1P5/EDG8 1 vial $399.00 Key Features: the cells. M00165 CHO-K1/NMUR1 Cells Ready-to-UseTM Neuromedin U NMU1 1 vial $399.00 M00168 CHO-K1/NTSR1 Cells Ready-to-UseTM Neurotensin NTS1 1 vial $399.00 • Reliability: Each batch of Ready-to-UseTM Frozen Cells are TM Bio-Reagent Center M00267 CHO-K1/OX1 Cells Ready-to-Use Orexin OX1 1 vial $399.00 M00179 CHO-K1/OXTR Cells Ready-to-UseTM Oxytocin OT/OXTR 1 vial $399.00 M00265 CHO-K1/PTAFR Cells Ready-to-UseTM Platelet Activating Factor PTAFR 1 vial $399.00 M00161 CHO-K1/NK1 Cells Ready-to-UseTM Tachykinin NK1/SPR 1 vial $399.00 Examples: M00171 CHO-K1/NK2 Cells Ready-to-UseTM Tachykinin NK2 1 vial $399.00 M00223 CHO-K1/V1B Cells Ready-to-UseTM Vasopressin V1B 1 vial $399.00 M00231 CHO-K1/PAC1/Gα15 Cells Ready-to-UseTM VIP/PACAP PAC1 1 vial $399.00
Quotations and Ordering:
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. C ustom Cell Line
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This diagram depicts the calcium dose-response of This diagram depicts the calcium dose-response of
ll Lin Neurotensin stimulation of CHO-K1/NTS1 Cells Ready-to-UseTM(●) Neurokinin A stimulation of CHO-K1/NK2 Cells Ready-to-UseTM(●) and Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 S Ce and non-treated cells (●). The result showing an increase in non-treated cells (●). The result showing an increase in intracellular ervice
m intracellular calcium was measured on a FlexStation fluorescent plate calcium was measured on a FlexStation fluorescent plate reader. Data Fax: 1-732-210-0262; 1-732-885-5878 o t reader. Data points represent the average +/- standard deviation of points represent the average +/- standard deviation of triplicate
triplicate determinations. determinations. Secured Web Server: https://www.genscript.com/ssl-bin/secure_data s Cus
- 85 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 86 - Bio-Assay Center —Flexible Solutions for Quality Assay and Screening!
Bio-Assay Center
GPCR Assay Services...... 90
Ion Channel Assay Services...... 92
Kinase Assay Services ...... 94
Protease Assay Services ...... 95
Miscellaneous Enzyme Assay Services...... 97 Cardiac Safety Testing Services ...... 99
One-stop Assay Services ...... 100
Email: [email protected] Tel: 1-732-885-9188 Bio-Assay Center GPCR Assay Services (http://www.genscript.com/bio-assay_center.html) (http://www.genscript.com/gpcr_assay_services.html ) Bio-Assay Center
Assay development plays a very important role in the identification of potential "hits", confirmation of the hits to lead compounds, and GPCR, G protein-coupled receptor, is the largest known GenScript not only provides a comprehensive collection of subsequent lead optimization, generating drug candidates for further development. GenScript has built a series of functional assay category of molecular targets with verified therapeutic value. It GPCR and parental cell lines, but also is able to construct platforms. These platforms implement the highest quality detection technology for early drug discovery. is comprised of approximately 600–1,000 members. Currently, advanced instrumentation and versatile assay platforms, a great percentage of pharmaceutical drugs targets GPCRs for GenScript brings a total solution for your biochemical and treatment of many diseases such as pain, asthma, cell-based GPCR assays and speed up your drug discovery. hypertension, as well as immunological and neurological Primary Assay HTS Conversion disorders. To expedite your drug discovery process, GenScript offers a variety of stable cell line products in our catalog, and a Compound Screening To meet the demands of GPCR screening and profiling, comprehensive collection of custom reagent services for gene,
Bio-Assay Center GPCRs, Ion Channels, Kinases, Proteases, Other Targets Assay Setup Showcases:
Secondary Assay Specificity Assay
Primary Assay GenScript has developed a series of specificity assay platforms integrating biochemical and cell-based method to cover a wide GenScript’s assay development process is based on stringent range of targets. Following clients’ requests to develop assay quality criteria and comprehensive controls. Equipped with on new targets, GenScript will offer a customized service based state-of-the-art instruments, GenScript has developed many on a feasibility study. advanced technology platforms that include fluorometry, electrophysiology, radioactivity, and in-cell Western assays. HTS Conversion Our scientific team has extensive experience in developing Fig. 1. Shown above are intracellular calcium responses from CHO-K1 Fig. 2. Shown above are the intracellular calcium responses of CHO-K1 pharmacologically sensitive assays for various drug target Facing a large compound library, GenScript can help clients G
cells stably expressing human NK3 Neurokinin receptors and from cells stably expressing human arginine vasopressin ( AVP) receptor 1B P convert the conventional primary assay into a high-throughput
classes that include GPCRs, ion channels, kinases, and C untransfected control cells. Cells were loaded with Calcium-4 and then and of untransfected control cells. Cells were loaded with Calcium-4 and proteases. Furthermore, GenScript’s scientists can skillfully screening (HTS) format and offer an assay validation for HTS. R Assa stimulated with the indicated concentrations of Neurokinin B. Calcium then stimulated with the indicated concentrations of AVP. Calcium integrate biochemical and cell-based assays to perform To meet your demand for HTS reagents, GenScript, as a world responses were recorded on a FlexStation plate reader. Data represent responses were recorded on a FlexStation plate reader. Data represent pharmacological profiling in order to improve safety and efficacy leading bio-reagent manufacturer, offers large-scale reagent the average +/- standard deviation of triplicate determinations. the average +/- standard deviation of triplicate determinations. y verview
of drug candidates with quality and speed. preparation services such as protein production and large-scale S O
cell culture. ervice Notably, our comprehensive services in recombinant protein Compound Screening
ervice production, antibody generation, peptide synthesis, and cell line s
S development allow you to take the advantages of our reputable in-house reagents supply to speed up assay development and Tailored to client’s specification, GenScript offers customizable Pricing: compound screening projects in a reliable, reproducible, and biochemical-based and cell-based compound screening cost-effective manner. services. Our compound screening service is conducted in Cat. No. Service Price 96-well plate or 384-well plate formats based on three SC1402 Custom GPCR Assay Services Quote Secondary Assay platforms: fluorescence, radioactivity, and in-cell Western. In addition, our patch clamp platform equipped with QPatch-16 After the compound library screening via the primary assay, screening station can run a medium throughput screening secondary assay is essential for hit confirmation and evaluation. (MTS) at about 1,000 data points per working day. Moreover, secondary assay can also be used to evaluate the improvement of pharmacokinetic performance following lead Secure Confidentiality optimization. GenScript has developed a panel of secondary assays focusing on both biochemical and cell-based modalities. Materials and information provided and sent to GenScript are With ample expertise in screening assays, we are dedicated to kept private by the strictest security measures. Assay deliver top-quality and fast-turnaround data analysis. outcomes and experimental data are proprietary to our clients and are also kept confidential. GenScript does not disclose Specificity Assay confidential information to third parties unless instructed to do so by the client. Even an efficacious candidate interacting with a therapeutic target may trigger unexpected or off-target effects in a living cell. Specificity assay is an effective method to evaluate the limitation and potential risk of a candidate compound.
- 89 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 90 - Ion Channel Assay Services
Quotations and Ordering: (http://www.genscript.com/ion_channel_assay_services.html) Bio-Assay Center
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose GenScript's proprietary assay techniques and novel expression and radioactivity to obtain top-quality and reliable data. Based the most convenient ordering method. For sample submission if applicable, please send to Bio-Assay Center, GenScript, 120 technologies can circumvent two of the major challenges on the methodologies, GenScript has gained extensive Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose thequotation form. associated with the search for novel ion channel drugs: low expertise on ion channel assays covering a wide range of ion protein expression levels and the limitations of traditional channel targets such as calcium channels, potassium channels, high-throughput screening (HTS). Our comprehensive ion sodium channels, and chloride channels. Quotation Form: Obtainable on our website channel services include assay development, primary screening, secondary screening, specificity assay and HTS To expedite your drug discovery process, GenScript offers a Email: [email protected] conversion. variety of stable cell line products in our catalog, and a comprehensive collection of custom reagent services for gene, Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 Equipped with state-of-the-art facilities, GenScript can offer protein, peptide, and antibody.
Bio-Assay Center various methodologies such as fluorometry, electrophysiology, Fax: 1-732-210-0262; 1-732-885-5878
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data Assay Setup Showcases: Ion C hannel Assa s Service y y
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Fig. 1. Dose-response relationship of TRPV1 agonist and antagonist in calcium influx assay. ervice
Changes in relative fluorescence units (RFU) are shown in (A) Dose-response relationship of capsaicin to 293/TRPV1 and 293 control cells. Cells were incubated with Calcium-4 and then stimulated with the indicated concentrations of capsaicin. (B) Dose-response relationship of capsazepine to 293/TRPV1 s GPCR Assa cells. Cells were incubated with Calcium-4 and indicated concentrations of capsazepine for 1 hour, then stimulated with 50 μM capsaicin. Calcium responses were recorded on a FlexStationIII plate reader. Data represent the average +/- standard deviation of triplicate determinations.
- 91 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 92 - Kinase Assay Services
(http://www.genscript.com/kinase_assay_services.html) Bio-Assay Center
Kinases play many important roles in cellular signaling Featured Services pathways and various other complex processes involving phosphorylation. The dysregulation of kinases is often • Assay Development associated with pathogenic pathways of many diseases, such • Assay Reagent Preparation as cancer, inflammatory diseases, neural disorders, and many • Compound Screening metabolic disorders. Protein kinases have extraordinary potential as drug targets. GenScript also provides Ki determination, substrate determination, and research collaboration services. GenScript provides a series of kinase assay services aiming to accelerate your drug discovery processes involving kinase
Bio-Assay Center targets.
Fig. 2. Dose-response relationship of P2X3 agonist and antagonist in membrane potential assay. Assay Setup Showcases:
Changes in relative fluorescence units (RFU) are shown in (A) Dose-response relationship of ATP to 293/P2X3 and 293 control cells. Cells were loaded with membrane potential sensitive dye (Molecular Devices) and then stimulated with the indicated concentrations of ATP. (B) Dose-response relationship of TN-ATP to 293/P2X3 cells. Cells were incubated with membrane potential sensitive dye (Molecular Devices) and indicated concentrations of TN-ATP for 45 minutes, then stimulated with 50 μM ATP. Calcium responses were recorded on a FlexStationIII plate reader. Data represent the average +/- standard deviation of triplicate determinations.
Pricing: s
Cat. No. Service Price K inase Assa Quote
Service SC1403 Custom Ion Channel Assay Services y y
Quotations and Ordering: Name: EGFR T790M Name: PKA S Source: Human Source: Human ervice Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Substrate: Abl1 peptide substrate (EAIYAAPFAKKK) Substrate: Kemptide peptide substrate (LRRASLG) s on Channel Assa on Channel the most convenient ordering method. For sample submission if applicable, please send to Bio-Assay Center, GenScript, 120 Reference Compound: Staurosprine Reference Compound: H-89 I Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. Method: HTRF Method: HTRF Testing: 8 conc. at n = 2 Testing: 8 conc. at n = 2
Quotation Form: Obtainable on our website
Email: [email protected] Pricing:
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 Cat. No. Service Price
SC1395 Custom Kinase Assay Services Quote Fax: 1-732-210-0262; 1-732-885-5878
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- 93 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 94 - Quotations and Ordering: Assay Setup Showcases: Bio-Assay Center
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method. For your sample submission if applicable, please send to Bio-Assay Center, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form.
Quotation Form: Obtainable on our website
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 Bio-Assay Center
Fax: 1-732-210-0262; 1-732-885-5878
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Name: MMP1 Name: Factor Xa Source: Human Source: Human Substrate: Substrate: CH OCO-D-CHA-Gly-Arg-pNA-AcOH Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 3 Reference Compound: NNGH Reference Compound: PPACK Protease Assay Services Method: Spectrofluorimetric Method: Spectrophotometric Testing: 10 conc. at n = 2 Testing: 8 conc. at n = 3 (http://www.genscript.com/protease_assay_services.html)
Approximately 500 putative proteases have been identified in Featured Services Pricing:
human genome. Through controlling complex proteolytic Protease Assa s activities, proteases participate in a wide range of physiological • Assay Development processes. Many of them have been utilized as targets for • Assay Reagent Preparation Cat. No. Service Price therapeutic intervention, such as Dipeptidyl Peptidase IV • Compound Screening SC1396 Custom Protease Assay Services Quote
Service (DPP-IV) and HIV-1 protease for diabetes and AIDS, y respectively. GenScript also provides Ki determination, substrate
determination, and research collaboration services. y
To speed up the drug discovery process that involves protease S Quotations and Ordering: ervice targets, GenScript has developed a protease assay panel for protease screening and profiling. Moreover, custom assay Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose s
Protease Assa development service is available to facilitate your drug the most convenient ordering method. For sample submission if applicable, please send to Bio-Assay Center, GenScript, 120 screening against protease targets of your interest. Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form.
Quotation Form: Obtainable on our website
Email: [email protected]
Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188
Fax: 1-732-210-0262; 1-732-885-5878
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- 95 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 96 - Miscellaneous Enzyme Assay Services
(http://www.genscript.com/enzymes_assay_services.html) Quotations and Ordering: Bio-Assay Center
Enzymes are attractive targets for pharmacological intervention, Featured Services: Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Bio-Assay Center owing to their essential roles in life processes and the most convenient ordering method. For sample submission if applicable, please send to , GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. pathophysiology. Because of their critical role in the study of • Assay Development drug metabolism, pharmacokinetics and toxicity reactions, the • Assay Reagent Preparation demand for the new enzymes assay development is increasing. • Compound Screening Quotation Form: Obtainable on our website GenScript's scientists are committed to develop a GenScript also provides Ki determination, substrate determination, comprehensive panel of assays. In addition to the popular and research collaboration services. Email: [email protected] kinase and protease assay panels, GenScript offers miscellaneous enzyme assay services, including but not limited Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 Bio-Assay Center to, lipases, oxidoreductases, deacetylases, CYP450 enzymes, and phosphodiesterases (PDE), many of which have been Fax: 1-732-210-0262; 1-732-885-5878 identified as prospect druggable entities. Secured Web Server: https://www.genscript.com/ssl-bin/secure_data
Assay Setup Showcases: M s iscellaneous Enz Service y y me Assa me Assa y y
S ervice
Name: Monoamine Oxidase A (MAO-A) Name: Monoamine Oxidase B (MAO-B) iscellaneous Enz s
M Source: Human Source: Human Substrate: Kynuramine Substrate: Kynuramine Reference Compound: Clorgyline Reference Compound: R-(-)-Deprenyl Hydrochloride Method: Spectrofluorimetric quantitation of 4-hydroxyquinoline Method: Spectrofluorimetric quantitation of 4-hydroxyquinoline Testing: 13 conc. at n = 2 Testing: 13 conc. at n = 2
Pricing:
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SC1406 Other Custom Assay Services Quote
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For cardiac safety assessment, various in vitro ion channel Featured Services Cat. No. Service Price assays are utilized at the early stage of drug discovery to avoid SC1309 Rapid Patch Screening Service Quote Quote potential expensive failure in late-stage drug development. Manual Patch Clamp Platform: SC1310 hERG IC50 Screening Assay Service Equipped with Qpatch 16 automated patch clamp and manual • hERG Manual Patch Clamp Testing Services patch clamp, GenScript offers cardiac safety testing services include hERG and Kv1.5 channels. Medicinal chemists and • Kv1.5 Manual Patch Clamp Testing Services toxicologists can efficiently have their compounds tested for potential cardiac liability using GenScript cardiac safety testing Automated Patch Clamp Platform: services. To offer more flexibility, more cardiac ion channels will • hERG ExpressPatch Testing Services be added in GenScript cardiac safety testing services. One-stop Assay Services Bio-Assay Center (www.genscript.com/one-stop_assay_services.html) Moreover, custom stable cell line service is available to your specifications. GenScript abides by its one-stop philosophy for assay development (GPCRs, ion channels), as well as biochemical development services, providing precision and convenience at and cell-based assay services. With our determination to competitive prices. GenScript offers an array of high-quality provide world-class profiling and screening services, and the Quotations and Ordering: reagents and services that are coordinated into a harmonious dedication of our experienced team, GenScript can design union for assay development and drug discovery. These specific assay to fit the needs of your projects. Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose services range from custom gene synthesis (drug target the most convenient ordering method. For sample submission if applicable, please send to Bio-Assay Center, GenScript, 120 expression), protein (drug targets including kinases, proteases, Please contact us to discuss how GenScript's One-stop Assay Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form. and more) and peptide production to custom cell line Services can support your research.
s Quotation Form: Obtainable on our website Quotations and Ordering:
Email: [email protected] O ne-stop Assa
Service Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose g Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 the most convenient ordering method. For sample submission if applicable, please send to Bio-Assay Center, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, U.S. Please complete and enclose the quotation form.
Testin Fax: 1-732-210-0262; 1-732-885-5878 y y
Secured Web Server: https://www.genscript.com/ssl-bin/secure_data Quotation Form: Obtainable on our website S ervice Email: [email protected] ardiac Safet s
C Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 hERG Manual Patch Clamp Testing Services Fax: 1-732-210-0262; 1-732-885-5878 Secured Web Server: https://www.genscript.com/ssl-bin/secure_data (http://www.genscript.com/hERG_safety_testing.html)
hERG testing for drug candidates is a crucial element of drug potentially lethal arrhythmia Torsades de Pointes (TdP), is now development, as an estimated 25–40% of all lead compounds well documented. Genscript helps our clients solve hERG show some level of hERG related toxicity. As a result, safety issues at early discovery stages and during preclinical regulatory agencies including FDA and EMEA require hERG/IKr development. pre-clinical safety data as part of any investigational new drug submission. The human cardiac delayed rectifier potassium GenScript’s premium technology and facilities enable the current (IKr) is carried by a channel encoded by the human delivery of this valuable service to you at the most affordable ether-a-go-go–related gene (hERG). Suppression of IKr by loss price in market. All assays are performed using our robust of function mutations in the hERG gene or by untoward drug hERG stable cell lines (HEK-293). block can prolong the QT interval and predispose patients to the
- 99 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 100 - Lead Optimization Center
—Integrated Solutions for Enhancing the Pipeline!
Lead Optimization Center
Medicinal Chemistry ...... 103
Discovery Biology ...... 104
Email: [email protected] Tel: 1-732-885-9188 Lead Optimization Center Discovery Biology (http://www.genscript.com/lead_optimization.html) Lead Optimization Center
GenScript's seasoned biologists, in alliance with partner medicinal chemists, offer both exploratory and full medicinal chemistry services, GenScript’s bio-assay center is capable of specificity and this stage, more sophisticated and reproducible readouts are and discovery biology services for assessing structure-activity relationships (SAR). The integrated services include custom organic secondary assays. Our assay services cover major classes of performed by more than 200 routine assays (cell-based or synthesis, peptide synthesis, intermediate and active drug substance production, high-value focused library design and synthesis, drug targets which include GPCR, ion channels, and enzymes biochemical). In addition, we can develop tailored assays primary and secondary assays, bioavailability, and toxicity assessment for advancing compounds from hit to lead, and lead optimization. (kinases, proteases, etc). GenScript’s lead optimization which rapidly turn hits into drug candidates. strategies are further validated by our secondary assays. At
Quotations and Ordering: Medicinal Chemistry Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose the most convenient ordering method.
With an experienced team in custom organic synthesis, custom peptide synthesis, intermediate and active drug substances Email: under GMP requirements, library design and synthesis, and [email protected] Lead Optimization Center medicinal chemistry, GenScript can provide cost-efficient alternatives for hit-to-lead and lead optimization in drug Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 discovery. Our libraries can be designed based on small molecular modeling and multi-parameter optimization, covering Fax: 1-732-210-0262; 1-732-885-5878 biochemical potency, efficacy, pharmacokinetics and pharmacodynamics, and toxicity. Our services include, but are Secured Web Server: https://www.genscript.com/ssl-bin/secure_data not limited to:
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Integrated Solutions for Enhancing the Pipeline!
- 103 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 104 - Antibody Drug Development Center —Comprehensive Solutions for Biologics Discovery!
Antibody Drug Development Center
Antibody Humanization ...... 107
Affinity Maturation ...... 107
Stable Cell Line Development and Antibody Production ...... 107
Supporting Services ...... 108
Email: [email protected] Tel: 1-732-885-9188 Antibody Drug Development Center Supporting Services (http://www.genscript.com/antibody_drug_development.html) Antibody Drug Development Center
As one of the world's leading biology contract research organizations, GenScript provides custom solutions to antibody humanization, Built on our assembly-line mode and one-stop solution, antibody sequencing service can speed and facilitate your affinity maturation, stable cell line development and antibody production. Our commitment to our customers' success is demonstrated GenScript's antibody drug development services are patent applications, database banking, and antibody by our proven track record of fast delivery and premium-quality, cost-effective services. Our commitment is also shown in our constant accelerated by our extensive supporting services that include therapeutics. efforts to provide the highest level of intellectual property protection for our clients. mAb sequencing, library construction, panning services, and • Antigen Production: Various antigens can be generated antigen production. by GenScript's custom peptide service or recombinant GenScript offers clients a comprehensive service line for antibody drug development, including antigen preparation, hybridoma • mAb Sequencing: GenScript's advanced technology offers protein expression service. We deliver target peptides or generation, monoclonal antibody sequencing, antibody humanization and affinity maturation, stable cell line development, and fast and professional sequencing services for your valuable proteins at > 95% purity. large-scale manufacturing of therapeutic antibody. monoclonal antibodies. Our ISO 9001:2000 certified
Services and Delivery Specifications: Antibody Humanization
Cat. No. ServiceSamples Submission Delivery Specifications Timeline The clinical utility of the murine monoclonal antibodies will elicit current CDR grafting approaches. All known heavy and light • VH, VL sequence and leader human anti-mouse antibody (HAMA) responses. To reduce the chain human germline genes are included in our proprietary 6 SC1408 Antibody sequencing • > 1x10 frozen hybridoma cells 3 Weeks immunogenicity of mAb from rodent sources and improve their sequence human framework library to ensure the effectiveness of our • Isotype information therapeutic efficacy, GenScript has developed and validated selective humanization strategy. • V-region storage for future use technologies and strategies for antibody humanization based on • > 1x106 frozen hybridoma • 0.5 mg of chimeric antibody Antibody Drug Development Center Chimeric antibody cells or sequences SC1409 • Antibody V-region sequence 8 Weeks generation • Target isotype information • V-region storage for future use • Specific antigen
• 0.5 mg of lead humanized mAb • > 1x106 frozen hybridoma • 0.5 mg of chimeric antibody Affinity Maturation cells or sequences • Plasmid DNA for Assessed SC1410 Antibody humanization • Target isotype information humanized mAbs by project • Specific antigen • Full report including High specificity and affinity of an antibody allows lower drug mutagenesis, structural analysis and prediction, and in silico sequences and results dosage to elicit more profound biological activity, which in turn, modeling based on our proprietary human antibody database. increases the therapeutic window and decreasing side-effects. This process, integrated with library screening by panning • 0.5 mg of affinity maturated GenScript's optimized antibody affinity maturation is achieved against the specific antigen, is a rapid pathway to obtain • > 1x106 frozen hybridoma antibodies • Plasmid DNA for affinity through three well-established approaches, including random affinity-matured antibody. SC1411 Antibody affinity maturation cells or sequences Assessed • Target isotype information maturated antibody by project • Specific antigen • Full report including sequences and results
Stable Cell Line Development and Antibody Production Quotations and Ordering:
Quotations and orders may be placed by email, phone, or fax with either a formal PO (Purchase Order) or credit card. Please choose Our extensive experience in mammalian cell culture has cell types (CHO,NS0, BHK, 293, and hybridomas, including the most convenient ordering method. allowed us to develop several highly effective proprietary adherent culture and suspension culture) are available for technologies for large-scale monoclonal antibody production. development and optimization. Our robust and scalable Over the years, we have developed and manufactured standard operating procedures in antibody identification and Email: [email protected] thousands of monoclonal antibodies. We provide a full range of purification can speed up and scale up process development supporting services in custom monoclonal antibody production and transfer. GenScript's state-of-the-art bioprocessing Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188 all the way from antibody engineering and cell line facilities enable us to quickly and reliably deliver for both small- establishment to downstream process development for and large-scale projects. Our bioreactor system platform gives Fax: 1-732-210-0262; 1-732-885-5878 fermentation and GLP-grade manufacturing. Flexible parental us antibody fermentation capacity of over 300 L.
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- 107 - Your Innovation Partner in Drug Discovery! Email: [email protected] Tel: 1-732-885-9188 - 108 - Bio-Reagent Center Custom Gene Services Custom Protein Services
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