Genscript Product Catalog 2013-2014 Genscript Product Catalog

Total Page:16

File Type:pdf, Size:1020Kb

Genscript Product Catalog 2013-2014 Genscript Product Catalog GenScript Product Catalog 2013-2014 GenScript Product Catalog www.genscript.com GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854USA Tel: 1-732-885-9188 / 1-732-885-9688 Toll-Free Tel: 1-877-436-7274 Fax: 1-732-210-0262 / 1-732-885-5878 Email: [email protected] Nucleic Acid Purification and Analysis Business Development Tel: 1-732-317-5088 PCR PCR and Cloning Email: [email protected] Protein Analysis Antibodies 2013-2014 Peptides Welcome to GenScript GenScript USA Incorporation, founded in 2002, is a fast-growing biotechnology company and contract research organization (CRO) specialized in custom services and consumable products for academic and pharmaceutical research. Built on our assembly-line mode, one-stop solutions, continuous improvement, and stringent IP protection, GenScript provides a comprehensive portfolio of products and services at the most competitive prices in the industry to meet your research needs every day. Over the years, GenScript’s scientists have developed many innovative technologies that allow us to maintain our position at the cutting edge of biological and medical research while offering cost-effective solutions for customers to accelerate their research. Our advanced expertise includes proprietary technology for custom gene synthesis, OptimumGeneTM codon optimization technology, CloneEZ® seamless cloning technology, FlexPeptideTM technology for custom peptide synthesis, BacPowerTM technology for protein expression and purification, T-MaxTM adjuvant and advanced nanotechnology for custom antibody production, as well as our ONE-HOUR WesternTM detection system and eStain® protein staining system. GenScript offers a broad range of reagents, optimized kits, and system solutions to help you unravel the mysteries of biology. We also provide a comprehensive portfolio of customized services that include Bio-Reagent, Bio-Assay, Lead Optimization, and Antibody Drug Development which can be effectively integrated into your value chain and your operations. All of these add up to maximum flexibility and convenience to meet your most demanding research needs. Our dedication to innovation and quality has helped us to establish strong partnerships with customers in over 70 countries, including many of the top pharmaceutical companies in the world. GenScript is headquartered in Piscataway, the heart of New Jersey’s pharmaceutical valley, with subsidiaries in Europe, Japan, and China. Navigating the Catalog The 2013-2014 product catalog is organized by scientific application and divided into six chapters. Browse through the catalog for information on popular products in molecular biology and cell biology and to see our featured products. We are confident you will find the 2013-2014 product catalog full of relevant and to see timely products and services to support your discovery or development program. Please visit our website at www.genscript.com to find new product information, cutting-edge tools, and resources, as well as to view the complete portfolio of products and services. As our offerings are constantly updated to meet your needs, please refer to our website for the most up-to-date information. Ordering and Technical Support Table of Contents For your convenience, you may place your order using any of the Technical Support following methods: order online, by email, phone, mail, or fax. 3 Our highly efficient customer service representatives will process Our technical support lines are open 24-hour Monday through your order quickly and accurately. Friday. Customer service representatives are here to assist you with ordering. Contact us from anywhere in the US: Order Online • Technical Support 1 Email: [email protected] Protein Analysis Online: You have two options for placing orders electronically: http://www.genscript.com/technical_support.html ....................................................... • Open a new account or login to your account and place your Protein Expression 23 • Customer Service order online at http://www.genscript.com ..................................... Email: [email protected] Protein Isolation and Purification 25 • Email your order to [email protected] Nucleic Acid Purification and Analysis Phone: 1-732-885-9188 / 1-732-885-9688 Protein Electrophoresis ................................................. 29 Toll-free phone: 1-877-436-7274 ................................................. Plasmid DNA Purification 3 Endotoxin Detection and Removal System ........................ 31 ............................................................ Order by Phone, Mail, or Fax PCR Purification 5 Protein Detection and Immunoassay ................................ 33 ............................................................... International Order Gel Extraction 6 Related Reagents for Protein Analysis .............................. 46 Please include the following information with your order: ...................................................... Nucleic Acid Markers 7 Catalog Proteins ......................................................... 51 • Name and phone number of the end user International customers should order directly from the nearest ................................. • Name of the principal investigator Custom Plasmid Preparation Services 9 Custom Protein Services ................................................ 65 authorized distributor of GenScript products. For the location of • Name and phone number of the purchasing agent the nearest distributor, refer to the distributor address list at • Catalog number and description of product http://www.genscript.com/distributor.html. International • Quantity and size of product customers in most countries can also order directly from • Shipping address GenScript without the assistance of a local distributor. The • Billing address estimated shipping price is based on FedEx international priority with 1 LB FedEx envelope and 3 LB box. Order by Phone 2 4 Call 1-732-885-9188 to place your order by speak directly with a helpful customer service representative. GenScript provides 24-hour service Monday through Friday. You can also place your order by voicemail anytime, day or PCR and Cloning Antibodies night. We accept Visa, MasterCard, American Express, and the ................................................................ TM American Express Corporate Purchasing Card. Green PCR 13 THE Elite Antibodies .................................................. 69 Routine PCR ............................................................... 14 Epitope Tag Antibodies .................................................. 74 Order by Mail PCR Cloning .............................................................. 16 Control Antibodies/Loading Control Antibodies .................... 78 GenPoolTM ORF Clones ................................................. 17 Primary Antibodies ....................................................... 79 If you would like to send us written confirmation of your order, Custom Molecular Biology Services .................................. Secondary Antibodies .................................................. please be sure it is clearly marked “Confirming Order—Do Not 18 99 Duplicate”. Custom Antibody Services ............................................ 100 GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 USA Order by Fax Fax your order to: 1-732-210-0262 / 1-732-885-5878 www.genscript.com - i - - ii - 5 THETHETMTM EliteElite AntibodiesAntibodies THE unmistakable, THE irreplaceable, and Peptides THE best quality in the industry β-Amyloid Peptides .................................................... 105 D Cosmetic Peptides...................................................... 107 Y K Pharmaceuticals ....................................................... 107 D D Peptide Building Blocks .............................................. D 107 H H H H D Other Peptides .......................................................... 111 H H K Related Biology Reagents ........................................... 124 Custom Peptide Services ............................................ 131 THETM Elite Antibody series are the most precise and effective antibodies that meet the most demanding research requirements. Appendix Ultra Sensitivity- More sensitive than most GF popular antibodies on the market Amino Acids Table ..................................................... 135 P GSG Atomic Weights of the Elements ..................................... 136 Ultra Specificity- No non-specific binding T ............................................. Genetic Markers in E.coli 137 Ultra Affinity- Ultra-low dissociation constant Kd Genetic Code Table ................................................... 139 ................ Ultra Reactivity- Recognizes target antigens in Determining the Molar Mass Using S20, W Values 139 Spectrophotometric Quantitation of DNA or RNA ............... 141 a wide range of species and expression systems Agarose and Polyacrylamide Gels .................................. 142 Ultra Clear Background Miscellaneous .......................................................... 142 Ultra Convenience- Wide applications and Alphabetical Index ..................................................... 145 comprehensive products can be used for a variety of research projects Ultra Stability- Stable lyophilized form for at least two years www.genscript.com - iii - - iv - Featured Products QuickClean II Plasmid Miniprep Kit CloneEZ® PCR Cloning Kit Up to 20 μg plasmid DNA with 99% purity No more restriction site headaches
Recommended publications
  • Characterisation and Partial Purification of a Novel Prohormone Processing Enzyme from Ovine Adrenal Medulla
    Volume 246, number 1,2, 44-48 FEB 06940 March 1989 Characterisation and partial purification of a novel prohormone processing enzyme from ovine adrenal medulla N. Tezapsidis and D.C. Parish Biochemistry Group, School of Biological Sciences, University of Sussex, Falmer, Brighton, Sussex, England Received 3 January 1989 An enzymatic activity has been identified which is capable of generating a product chromatographically identical with adrenorphin from the model substrate BAM 12P. This enzyme was purified by gel filtration and ion-exchange chromatog- raphy and characterised as having a molecular mass between 30 and 45 kDa and an acidic pL The enzyme is active at the acid pH expected in the secretory vesicle interior and is inhibited by EDTA, suggesting that it is a metalloprotease. This activity could not be mimicked by incubation with lysosomal fractions and it meets the criteria to be considered as a possible prohormone processing enzyme. Prohormone processing; Adrenorphin; Secretory vesiclepurification 1. INTRODUCTION The purification of an endopeptidase responsi- ble for the generation of adrenorphin was under- Active peptide hormones are released from their taken, using the ovine adrenal medulla as a source. precursors by endoproteolytic cleavage at highly Adrenorphin is known to be located in the adrenal specific sites. The commonest of these is cleavage medulla of all species so far investigated [6]. at pairs of basic residues such as lysine and Secretory vesicles (also known as chromaffin arginine [1,2]. However another common class of granules) were isolated as a preliminary purifica- processing sites are known to be at single arginine tion step, since it is known that prohormone pro- residues, adjacent to a proline [3].
    [Show full text]
  • Entropy-Driven Binding of Opioid Peptides Induces a Large Domain Motion in Human Dipeptidyl Peptidase III
    Entropy-driven binding of opioid peptides induces a large domain motion in human dipeptidyl peptidase III Gustavo A. Bezerraa, Elena Dobrovetskyb, Roland Viertlmayra, Aiping Dongb, Alexandra Binterc, Marija Abramic´d, Peter Macherouxc, Sirano Dhe-Paganonb,e, and Karl Grubera,1 aInstitute of Molecular Biosciences, University of Graz, A-8010 Graz, Austria; eDepartment of Physiology and bStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada M5G 1L7; cInstitute of Biochemistry, Graz University of Technology, A-8010 Graz, Austria; and dDivision of Organic Chemistry and Biochemistry, Ruđer Boskovic Institute, 10002 Zagreb, Croatia Edited by William W. Bachovchin, Tufts University School of Medicine, Boston, MA, and accepted by the Editorial Board March 9, 2012 (received for review November 2, 2011) Opioid peptides are involved in various essential physiological of action compared with morphine, spinorphin is an analgesic, processes, most notably nociception. Dipeptidyl peptidase III (DPP potentially useful for pain treatment in morphine-resistant cases III) is one of the most important enkephalin-degrading enzymes (14). This opioid peptide was also shown to be a potent and se- associated with the mammalian pain modulatory system. Here we lective antagonist of the receptor P2X3, which is involved in pain describe the X-ray structures of human DPP III and its complex with signaling in chronic inflammatory nociception and neuropathic the opioid peptide tynorphin, which rationalize the enzyme’s sub- pain due to nerve injury (15). strate specificity and reveal an exceptionally large domain motion Tynorphin (Val-Val-Tyr-Pro-Trp), a synthetic, truncated form upon ligand binding. Microcalorimetric analyses point at an en- of spinorphin, is a highly specific inhibitor of DPP III and was tropy-dominated process, with the release of water molecules shown to induce an even more potent antinociceptive effect from the binding cleft (“entropy reservoir”) as the major thermo- (14, 16).
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 9,353,350 B2 Kobayashi Et Al
    US009353350B2 (12) United States Patent (10) Patent No.: US 9,353,350 B2 Kobayashi et al. (45) Date of Patent: May 31, 2016 (54) METHOD FOR PRODUCING MULTIPOLAR 2012fO149053 A1 6, 2012 Yoshida et al. CELL 2013,0323,776 A1 12/2013 Yoshida et al. 2015. OO18286 A1* 1/2015 Kobayashi et al. .......... 514, 19.3 (71) Applicant: TOAGOSEICO.,LTD., Tokyo (JP) FOREIGN PATENT DOCUMENTS (72) Inventors: Nahoko Kobayashi, Tsukuba (JP); CN 1763O82 A 4/2006 Tetsuhiko Yoshida, Tsukuba (JP); Yuki DE 102009021681 A1 11, 2010 Kobayashi, Fujisawa (JP) WO O3O24408 A2 3, 2003 WO O3O37172 A2 5, 2003 WO 2004.005472 A2 1, 2004 (73) Assignee: TOAGOSEICO. LTD., Tokyo (JP) WO 2004/020457 A2 3, 2004 WO 2007/004869 A2 1, 2007 (*) Notice: Subject to any disclaimer, the term of this WO 2007056188 A1 5/2007 patent is extended or adjusted under 35 WO 2008/081812 A1 T 2008 WO WO 2009,093692 A1 T 2009 U.S.C. 154(b) by 0 days. WO WO 2011/O13698 A1 2, 2011 (21) Appl. No.: 14/366,971 WO WO 2011/O13699 A1 2, 2011 (22) PCT Filed: Dec. 20, 2012 OTHER PUBLICATIONS Paradis-Bleau et al., “Peptide inhibitors of the essential cell division (86). PCT No.: PCT/UP2O12AO83110 protein FtsA'. Protein Engineering, Design & Selection, 2005, pp. S371 (c)(1), 85-91, vol. 18, No. 2, Oxford University Press. (2) Date: Jun. 19, 2014 Paradis-Bleau et al., “Identification of Pseudomonas aeruginosa FtsZ. peptide inhibitors as a tool for development of novel antimicro bials”, Journal of Antimicrobial Chemotherapy, Jun. 2004, pp.
    [Show full text]
  • PRODUCT INFORMATION Spinorphin Item No
    PRODUCT INFORMATION Spinorphin Item No. 29914 NH CAS Registry No.: 137201-62-8 Formal Name: L-leucyl-L-valyl-L-valyl-L-tyrosyl-L- O H O N prolyl-L-tryptophyl-L-threonine N OH O N H Synonyms: Leu-Val-Val-Tyr-Pro-Trp-Thr, O OH H LVVYPWT O O N MF: C45H64N8O10 N N O FW: 877.0 H H NH2 Purity: ≥95% UV/Vis.: λmax: 222 nm Supplied as: A crystalline solid Storage: -20°C OH Stability: ≥2 years Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis. Laboratory Procedures Spinorphin is supplied as a crystalline solid. A stock solution may be made by dissolving the spinorphin in the solvent of choice, which should be purged with an inert gas. Spinorphin is soluble in organic solvents such as DMSO and dimethyl formamide. The solubility of spinorphin in these solvents is approximately 30 mg/ml. Description Spinorphin is a heptapeptide inhibitor of the enkephalin-degrading enzymes aminopeptidase, dipeptidyl aminopeptidase, angiotensin-converting enzyme (ACE), and enkephalinase (IC50s = 3.3, 1.4, 2.4, and 10 µg/ml, respectively, for monkey brain enzymes).1 It is selective for these enzymes over human serum aminopeptidase A (IC50 = >100 µg/ml), as well as porcine kidney aminopeptidase B, aminopeptidase M, dipeptidyl peptidase 1 (DPP-1), DPP-2, DPP-3, and DPP-4 (IC50s = >55 µg/ml for all). Spinorphin inhibits chemotaxis, production of reactive oxygen species (ROS), and exocytosis of glucuronidase and collagenase in polymorphonuclear neutrophils (PMNs). It potentiates enkephalin-induced action potentials in rat hippocampal slices.
    [Show full text]
  • Biological Redundancy of Endogenous GPCR Ligands in the Gut and the Potential for Endogenous Functional Selectivity
    REVIEW ARTICLE published: 28 November 2014 doi: 10.3389/fphar.2014.00262 Biological redundancy of endogenous GPCR ligands in the gut and the potential for endogenous functional selectivity Georgina L. Thompson1, Meritxell Canals1 and Daniel P.Poole1,2 * 1 Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Parkville, VIC, Australia 2 Department of Anatomy and Neuroscience, The University of Melbourne, Parkville, VIC, Australia Edited by: This review focuses on the existence and function of multiple endogenous agonists Dominique Massotte, Institut des of the somatostatin and opioid receptors with an emphasis on their expression in the Neurosciences Cellulaires et Intégratives, France gastrointestinal tract. These agonists generally arise from the proteolytic cleavage of prepropeptides during peptide maturation or from degradation of peptides by extracellular Reviewed by: Jakub Fichna, Medical University of or intracellular endopeptidases. In other examples, endogenous peptide agonists for the Lodz, Poland same G protein-coupled receptors can be products of distinct genes but contain high Pamela J. Hornby, Johnson & sequence homology. This apparent biological redundancy has recently been challenged Johnson, USA by the realization that different ligands may engender distinct receptor conformations *Correspondence: linked to different intracellular signaling profiles and, as such the existence of distinct Daniel P.Poole, Drug Discovery Biology, Monash Institute of ligands may underlie mechanisms to finely tune physiological responses. We propose that Pharmaceutical Sciences, 381 Royal further characterization of signaling pathways activated by these endogenous ligands will Parade, Parkville, VIC 3052, Australia provide invaluable insight into the mechanisms governing biased agonism. Moreover, these e-mail: [email protected] ligands may prove useful in the design of novel therapeutic tools to target distinct signaling pathways, thereby favoring desirable effects and limiting detrimental on-target effects.
    [Show full text]
  • Sized Neuropeptides
    M ETHODS IN MOLECULAR BIOLOGY™ Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Neuropeptides Methods and Protocols Edited by Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria, Università degli Studi di Torino, Grugliasco, TO, Italy; Istituto Nazionale di Neuroscienze (INN), Università degli Studi di Torino, Grugliasco, TO, Italy Editor Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria Università degli Studi di Torino and Istituto Nazionale di Neuroscienze (INN) Università degli Studi di Torino Grugliasco, TO, Italy [email protected] Please note that additional material for this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-309-7 e-ISBN 978-1-61779-310-3 DOI 10.1007/978-1-61779-310-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011936011 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights.
    [Show full text]
  • Formylpeptide Receptor N Antagonist at The
    The Endogenous Opioid Spinorphin Blocks fMet-Leu-Phe-Induced Neutrophil Chemotaxis by Acting as a Specific Antagonist at the N-Formylpeptide Receptor This information is current as Subtype FPR of October 1, 2021. Thomas S. Liang, Ji-Liang Gao, Omid Fatemi, Mark Lavigne, Thomas L. Leto and Philip M. Murphy J Immunol 2001; 167:6609-6614; ; doi: 10.4049/jimmunol.167.11.6609 Downloaded from http://www.jimmunol.org/content/167/11/6609 References This article cites 48 articles, 13 of which you can access for free at: http://www.jimmunol.org/content/167/11/6609.full#ref-list-1 http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists by guest on October 1, 2021 • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2001 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Endogenous Opioid Spinorphin Blocks fMet-Leu-Phe-Induced Neutrophil Chemotaxis by Acting as a Specific Antagonist at the N-Formylpeptide Receptor Subtype FPR Thomas S.
    [Show full text]
  • Peptide Handbook a Guide to Peptide Design and Applications in Biomedical Research
    Peptide Handbook A Guide to Peptide Design and Applications in Biomedical Research First Edition www.GenScript.com GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 USA Phone: 1-732-885-9188 Toll-Free: 1-877-436-7274 Fax: 1-732-885-5878 Table of Contents The Universe of Peptides Reliable Synthesis of High-Quality Peptides Molecular structure 3 by GenScript Characteristics 5 Categories and biological functions 8 Analytical methods 10 Application of Peptides Research in structural biology 12 Research in disease pathogenesis 12 Generating antibodies 13 FlexPeptideTM Peptide Synthesis Platform which takes advantage of the latest Vaccine development 14 peptide synthesis technologies generates a large capacity for the quick Drug discovery and development 15 synthesis of high-quality peptides in a variety of lengths, quantities, purities Immunotherapy 17 and modifications. Cell penetration-based applications 18 Anti-microorganisms applications 19 Total Quality Management System based on multiple rounds of MS and HPLC Tissue engineering and regenerative medicine 20 analyses during and after peptide synthesis ensures the synthesis of Cosmetics 21 high-quality peptides free of contaminants, and provides reports on peptide Food industry 21 solubility, quality and content. Synthesis of Peptides Diverse Delivery Options help customers plan their peptide-based research Chemical synthesis 23 according to their time schedule and with peace of mind. Microwave-assisted technology 24 ArgonShield™ Packing eliminates the experimental variation caused by Ligation technology 26 oxidization and deliquescence of custom peptides through an innovative Recombinant technology 28 Modifications packing and delivery technology. 28 Purification 30 Expert Support offered by Ph.D.-level scientists guides customers from Product identity and quality control 31 peptide design and synthesis to reconstitution and application.
    [Show full text]
  • Opioids, Neutral Endopeptidase, Its Inhibitors and Cancer: Is There a Relationship Among Them?
    Arch. Immunol. Ther. Exp. DOI 10.1007/s00005-014-0311-0 REVIEW ARTICLE Opioids, Neutral Endopeptidase, its Inhibitors and Cancer: Is There a Relationship among them? Magdalena Mizerska-Dudka • Martyna Kandefer-Szerszen´ Received: 11 March 2014 / Accepted: 18 June 2014 Ó The Author(s) 2014. This article is published with open access at Springerlink.com Abstract The role of endogenous animal opioids in the CKI Cyclin dependent inhibitory kinases biology of cancer is widely recognized but poorly under- ECM Extracellular matrix stood. This is, among others, because of the short half-life FAK Focal adhesion kinase of these peptides, which are quickly inactivated by endo- GPI-complex Glycosyl phosphatidyl inositol complex peptidases, e.g., neutral endopeptidase (NEP, CD10). It has MAP kinases Mitogen-activated protein kinases been established that NEP is engaged in the modulation of mRNA Messenger RNA the tumor microenvironment, among others that of colon NEP Neutral endopeptidase cancer, by exerting influence on cell growth factors, the NK Natural killer cells extracellular matrix and other biologically active sub- OGF Opioid growth factor stances. Although there are some discrepancies among the OGFr Opioid growth factor receptor findings on the role of both opioids and NEP in cancer PROL1 Proline rich, lacrimal 1 development, authors agree that their role seems to depend PTEN Phosphatase and tensin homolog deleted on the origin, stage and grade of tumor, and even on the on chromosome Ten method of examination. Moreover, recently, natural SGP-T Submandibular gland peptide-T inhibitors of NEP, such as sialorphin, opiorphin and spin- SMR1 Submandibular rat1 protein orphin have been detected.
    [Show full text]
  • CEM Peptide Synthesis Brochure
    Unparalleled Peptide Synthesis cempeptides.com Contents CEM Overview 2 Innovations in Microwave Peptide Synthesis 2 Founding Fathers 3 Corporate Legacy Chemistry Technologies 4 HE-SPPS 6 CarboMAXTM 8 One Pot Coupling/Deprotection Peptide Synthesizers 10 Sequential vs Parallel 11 Synthesizer Comparison 12 Discover BioTM 12 Liberty LiteTM 13 Liberty BlueTM 13 Liberty Blue HT12TM 14 Liberty PRIMETM 16 Accessories & Upgrades Peptide Cleavage 17 Razor® SPPS Reagents 18 Fmoc Amino Acids 19 Oxyma Pure 19 Resins (ProTide™, Polystyrene) Large Scale Microwave Peptide Synthesis 22 Liberty PRO™ Customers 24 Testimonials 25 Support CEM Overview Innovations in Microwave Peptide Synthesis 1978 CEM Corporation founded as a new company, based on microwave laboratory instrumentation 2001 CEM launches a single mode microwave system for chemical synthesis 2003 CEM develops the world’s first automated microwave peptide synthesizer1 2007 CEM publishes research for optimized methods for aspartimide formation and epimerization under microwave SPPS2 2013 Liberty Blue™ peptide synthesizer developed based on High Efficiency Solid Phase Peptide Synthesis (HE-SPPS) 2014 HE-SPPS methodology published3 2016 CEM launches new universal load resins eliminating the need for pre-loaded resins historically used 2016 CEM offers the world’s first large-scale microwave peptide synthesis, with capabilities of up to 500 grams of a purified peptide, in a single batch 2016 CEM develops improved carbodiimide coupling methods for peptide synthesis at elevated temperature (CarboMAX™) 2017 CEM develops a novel one-pot coupling/ deprotection process reducing SPPS cycle time and waste usage (Liberty PRIME™) Founding Fathers (circa 1980) Chemist: Dr. Michael J. Collins (Middle) Electrical Engineer: Ron Goetchius (Left) Mechanical Engineer: Bill Cruse Jr.
    [Show full text]
  • Opiorphin- Inhibitors of Enkephalins by Inactivating Ectopeptidases
    Human Journals Review Article April 2020 Vol.:18, Issue:1 © All rights are reserved by GAURAV M. PRAJAPATI Opiorphin- Inhibitors of Enkephalins by Inactivating Ectopeptidases Keywords: Injury, opiorphin, enkephalins, growth, glutamine, NEP & AP-N Sensitive, bioavailability ABSTRACT GAURAV M. PRAJAPATI*1 The unpleasant physical sensation caused by illness or injury is *1Department of pharmacology, Kasturi Shikshan called pain and substances which are used in reducing or giving Sanstha’s college of Pharmacy, Pune-412208, relief in pain are called analgesics. Human saliva is a natural pain Maharashtra, India killer which is six times more potent than morphine and consists of enkephalins inhibiting enzymes in human neutral ecto Submission: 24 March 2020 endopeptidase and ectoamino peptidase. Without directly Accepted: 31 March 2020 interacting with an opioid receptor opiorphin extracts with Published: 30 April 2020 antinociceptive effect by activating of mu and delta receptor. Opiorphin and its derivatives treated with the disorder that included pain and mood-related disorder. Gene coding opiorphin can be the biomarker of erectile dysfunction. Glutamine is the first position that is crucial for its pharmacological actions on gastrointestinal motility. Opiorphin play physiological roles in www.ijppr.humanjournals.com follicular growth, ovulation, and embryo implantation processes including maternal-fetal including control of the local concentration of NEP & AP- NSensitive. It also helps in the modulation of lachrymal homeostatic by increasing the bioavailability of enkephalins. www.ijppr.humanjournals.com INTRODUCTION Pain is defined as an unpleasant emotional and sensory experience associated with actual and potential tissue damage1. Pain is rarely of two types chronic and acute that differs from each other based on etiology and pathophysiology.
    [Show full text]
  • Five Decades of Research on Opioid Peptides: Current Knowledge and Unanswered Questions
    Molecular Pharmacology Fast Forward. Published on June 2, 2020 as DOI: 10.1124/mol.120.119388 This article has not been copyedited and formatted. The final version may differ from this version. File name: Opioid peptides v45 Date: 5/28/20 Review for Mol Pharm Special Issue celebrating 50 years of INRC Five decades of research on opioid peptides: Current knowledge and unanswered questions Lloyd D. Fricker1, Elyssa B. Margolis2, Ivone Gomes3, Lakshmi A. Devi3 1Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; E-mail: [email protected] 2Department of Neurology, UCSF Weill Institute for Neurosciences, 675 Nelson Rising Lane, San Francisco, CA 94143, USA; E-mail: [email protected] 3Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, Annenberg Downloaded from Building, One Gustave L. Levy Place, New York, NY 10029, USA; E-mail: [email protected] Running Title: Opioid peptides molpharm.aspetjournals.org Contact info for corresponding author(s): Lloyd Fricker, Ph.D. Department of Molecular Pharmacology Albert Einstein College of Medicine 1300 Morris Park Ave Bronx, NY 10461 Office: 718-430-4225 FAX: 718-430-8922 at ASPET Journals on October 1, 2021 Email: [email protected] Footnotes: The writing of the manuscript was funded in part by NIH grants DA008863 and NS026880 (to LAD) and AA026609 (to EBM). List of nonstandard abbreviations: ACTH Adrenocorticotrophic hormone AgRP Agouti-related peptide (AgRP) α-MSH Alpha-melanocyte stimulating hormone CART Cocaine- and amphetamine-regulated transcript CLIP Corticotropin-like intermediate lobe peptide DAMGO D-Ala2, N-MePhe4, Gly-ol]-enkephalin DOR Delta opioid receptor DPDPE [D-Pen2,D- Pen5]-enkephalin KOR Kappa opioid receptor MOR Mu opioid receptor PDYN Prodynorphin PENK Proenkephalin PET Positron-emission tomography PNOC Pronociceptin POMC Proopiomelanocortin 1 Molecular Pharmacology Fast Forward.
    [Show full text]