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Sarcoma Fusions 101 Flyer Get more from your sarcoma analysis Detect known and novel fusion partners for fast insights with NGS Sarcomas are rare but complex Gene fusion partners matter Sarcomas are cancers found in connective tissues such Some genes, such as EWSR1, are particularly as bone and soft tissues. While rare—with more than associated with sarcomas and have multiple fusion 15,000 new diagnoses each year in the US—27% are partners. These varied fusion partnerships result in found in children and young adults under age 30.1,2 different sarcoma subtypes.5 DDIT3 Myxoid Liposarcoma ERG FLI1 ETV1 Ewing's Sarcoma E1AF Gene FEV fusion EWSR1 ATF1 Clear Cell Sarcoma WT1 Desmoplastic Small Cell Tumor ZSG Askin Like CD99 Negative Sarcoma Translocation TEC Extraskeletal Myxoid Chondrosarcoma NMP4 Acute Lymphatic Leukemia ~1/3 of sarcomas are caused by chromosomal Sarcomas include more than 50 subtypes based on translocations that lead to gene fusions.3,4 the cell of origin.6 Accurate subtype identification is essential for implementing insights Detecting sarcoma subtypes is more complicated than identifying the cell of origin, but conventional molecular analysis methods can have limitations. Fluorescence in situ hybridization (FISH) • Usually interrogates only one pair of genes • Translocations of one gene can occur with multiple fusion partners, resulting in incorrect sarcoma subtype identification Reverse transcription polymerase chain reaction (RT-PCR) • Requires knowledge of both partners and anticipated break points TruSight™ RNA Fusion Panel: Comprehensive coverage in a single assay Next-generation sequencing (NGS) covers hundreds of fusion-associated genes—and can detect novel fusions—so you can identify chromosomal abnormalities in the first try. For Research Use Only. Not for use in diagnostic procedures. The TruSight RNA Fusion Panel Known and novel fusions. Fast and accurate insights. TruSight RNA Fusion Panel covers a broad range of fusion-associated genes Novel without prior knowledge >500 142 Fusions Fusion of specic translocations Fusion-associated genes previously found in sarcomas4 Detection or chromosomal breakpoints Identify fusions associated with common sarcomas Ewing’s Sarcoma Alveolar Rhabdomyosarcoma Synovial Sarcoma Myxoid Liposarcoma Other subtypes PAX7 TAF15 SSX1 WT1 COL1A1 TFG FOX01 SS18 TCF12 SS18L1 NR4A3 ETV4 SSX2 PDGFB PATZ1 ATF1 PAX3 SSX4 CLTC ATIC EWSR1 CREB3L1 SEC31A FLI1 DDIT3 FOX04 CREB3L2 NCOA1 TPM4 ALK CARS ASPSCR1 FEV ERG NTRK3 ETV1 FUS RANBP2 TPM3 TFE3 ETV6 Round cell tumors Spindle cell tumors The TruSight RNA Fusion Panel provides a reproducible and economical solution7 for identifying gene fusions in sarcomas. Learn More For more information about the TruSight RNA Fusion Panel, visit illumina.com/RNAFusion. References 1. Estimated New Cases, 2017. American Cancer Society. https://cancerstatisticscenter.cancer.org/#/data-analysis/module/BmVYeqHT?type=barGraph. Accessed June 23, 2017. 2. SEER Cancer Statistics Review 1975-2004: Table I-10, Age Distribution at Diagnosis and Death. National Cancer Institute Surveillance, Epidemiology, and End Results Program. https://seer.cancer.gov/archive/csr/1975_2004/results_merged/topic_age_dist.pdf. Accessed July 17, 2017. 3. Taylor BS, Barretina J, Maki RG, Antonescu CR, Singer S, Ladanyi M. Advances in sarcoma genomics and new therapeutic targets. Nat Rev Cancer. 2011;11(8):541-557. 4. Mertens F, Antonescu CR, Mitelman F, et al. Gene fusions in soft tissue tumors: Recurrent and overlapping pathogenetic themes. Genes Chromosomes Cancer. 2016;55(4):291-310. 5. Åman P. Fusion Oncogenes in tumor development. Semin Cancer Biol. 2005;15(3):236-243. 6. What is a soft tissue sarcoma? American Cancer Society. https://www.cancer.org/cancer/soft-tissue-sarcoma/about/soft-tissue-sarcoma.html. Accessed June 23, 2017. 7. Data calculations on file. Illumina, Inc. 2017. For Research Use Only. Not for use in diagnostic procedures. © 2017 Illumina, Inc. All rights reserved. Pub. No. 1170-2017-011.
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