Anti-SIT Purified Cat

Total Page:16

File Type:pdf, Size:1020Kb

Anti-SIT Purified Cat EXBIO Praha, a.s. • Nad Safinou II 341 • 252 50 Vestec • Czech Republic [email protected][email protected][email protected] • www.exbio.cz Technical Data Sheet Product Anti-SIT Purified Cat. Number/Size 11-345-C025 0.025 mg 11-345-C100 0.1 mg For Research Use Only. Not for use in diagnostic or therapeutic procedures. Antigen SIT Clone SIT-01 Format Purified Reactivity Human Application FC, IP, WB Application details Flow cytometry: Intracellular staining. Western blotting: SIT migrates as an approximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidase treatment. Isotype Mouse IgG1 Specificity The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT. Other names SIT1 Immunogen Bacterially produced recombinant intracellular fragment of human SIT. Entrez Gene ID 27240 Gene name SIT1 NCBI Full Gene Name signaling threshold regulating transmembrane adapt UniProt ID Q9Y3P8 Concentration 1 mg/ml Preparation Purified by protein-A affinity chromatography Formulation Phosphate buffered saline (PBS) solution with 15 mM sodium azide Storage and handling Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Images and References www.exbio.cz The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. Revision date: 2021-06-08.
Recommended publications
  • Genetic Analysis of Retinopathy in Type 1 Diabetes
    Genetic Analysis of Retinopathy in Type 1 Diabetes by Sayed Mohsen Hosseini A thesis submitted in conformity with the requirements for the degree of Doctor of Philosophy Institute of Medical Science University of Toronto © Copyright by S. Mohsen Hosseini 2014 Genetic Analysis of Retinopathy in Type 1 Diabetes Sayed Mohsen Hosseini Doctor of Philosophy Institute of Medical Science University of Toronto 2014 Abstract Diabetic retinopathy (DR) is a leading cause of blindness worldwide. Several lines of evidence suggest a genetic contribution to the risk of DR; however, no genetic variant has shown convincing association with DR in genome-wide association studies (GWAS). To identify common polymorphisms associated with DR, meta-GWAS were performed in three type 1 diabetes cohorts of White subjects: Diabetes Complications and Control Trial (DCCT, n=1304), Wisconsin Epidemiologic Study of Diabetic Retinopathy (WESDR, n=603) and Renin-Angiotensin System Study (RASS, n=239). Severe (SDR) and mild (MDR) retinopathy outcomes were defined based on repeated fundus photographs in each study graded for retinopathy severity on the Early Treatment Diabetic Retinopathy Study (ETDRS) scale. Multivariable models accounted for glycemia (measured by A1C), diabetes duration and other relevant covariates in the association analyses of additive genotypes with SDR and MDR. Fixed-effects meta- analysis was used to combine the results of GWAS performed separately in WESDR, ii RASS and subgroups of DCCT, defined by cohort and treatment group. Top association signals were prioritized for replication, based on previous supporting knowledge from the literature, followed by replication in three independent white T1D studies: Genesis-GeneDiab (n=502), Steno (n=936) and FinnDiane (n=2194).
    [Show full text]
  • Towards Relating the Evolution of the Gene Repertoire in Mammals to Tissue Specialisation
    Towards Relating the Evolution of the Gene Repertoire in Mammals to Tissue Specialisation Shiri Freilich Wolfson College This dissertation is submitted to the University of Cambridge for the degree of Doctor of Philosophy 21 December 2006 To Leon, who was the wind blowing in my sails, in the deep blue sea of this journey of ours. This Thesis is the result of my own work and includes nothing which is the outcome of work done in collaboration except where specifically indicated in the text. This Thesis does not exceed the specified length limit of 300 pages as defined by the Biology Degree Committee. This Thesis has been typeset in 12pt font according to the specifications defined by the Board of Graduate Studies and the Biology Degree Committee. 3 Summary: Towards Relating the Evolution of the Gene Repertoire in Mammals to Tissue Specialisation The sequencing efforts of recent years have provided a rich source of data for investigating how gene content determines similarity and uniqueness in a species’ phenotype. Work described in this PhD Thesis attempts to relate innovations in the gene repertoire along the mammalian lineage to the most obvious phenotypic characteristic of animals: the appearance of highly differentiated tissue types. Several different approaches, outlined below, have been followed to address some aspects of this problem. Initially, a comprehensive study of the pattern of expansion of the complement of enzymes in various species was performed in order to obtain a better view of the principles underlying the expansion of the gene repertoire in mammals. Although several studies have described a tendency toward an increase in sequence redundancy in mammals, not much is known about the way such sequence redundancy reflects functional redundancy.
    [Show full text]
  • Constraints and Consequences of the Emergence of Amino Acid Repeats in Eukaryotic Proteins
    ARTICLES Constraints and consequences of the emergence of amino acid repeats in eukaryotic proteins Sreenivas Chavali1 , Pavithra L Chavali1,2,5, Guilhem Chalancon1,5, Natalia Sanchez de Groot1, Rita Gemayel3,4, Natasha S Latysheva1, Elizabeth Ing-Simmons1, Kevin J Verstrepen3,4, Santhanam Balaji1 & M Madan Babu1 Proteins with amino acid homorepeats have the potential to be detrimental to cells and are often associated with human diseases. Why, then, are homorepeats prevalent in eukaryotic proteomes? In yeast, homorepeats are enriched in proteins that are essential and pleiotropic and that buffer environmental insults. The presence of homorepeats increases the functional versatility of proteins by mediating protein interactions and facilitating spatial organization in a repeat-dependent manner. During evolution, homorepeats are preferentially retained in proteins with stringent proteostasis, which might minimize repeat-associated detrimental effects such as unregulated phase separation and protein aggregation. Their presence facilitates rapid protein divergence through accumulation of amino acid substitutions, which often affect linear motifs and post-translational-modification sites. These substitutions may result in rewiring protein interaction and signaling networks. Thus, homorepeats are distinct modules that are often retained in stringently regulated proteins. Their presence facilitates rapid exploration of the genotype– phenotype landscape of a population, thereby contributing to adaptation and fitness. Repetitive sequences are
    [Show full text]
  • The Transition from Primary Colorectal Cancer to Isolated Peritoneal Malignancy
    medRxiv preprint doi: https://doi.org/10.1101/2020.02.24.20027318; this version posted February 25, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license . The transition from primary colorectal cancer to isolated peritoneal malignancy is associated with a hypermutant, hypermethylated state Sally Hallam1, Joanne Stockton1, Claire Bryer1, Celina Whalley1, Valerie Pestinger1, Haney Youssef1, Andrew D Beggs1 1 = Surgical Research Laboratory, Institute of Cancer & Genomic Science, University of Birmingham, B15 2TT. Correspondence to: Andrew Beggs, [email protected] KEYWORDS: Colorectal cancer, peritoneal metastasis ABBREVIATIONS: Colorectal cancer (CRC), Colorectal peritoneal metastasis (CPM), Cytoreductive surgery and heated intraperitoneal chemotherapy (CRS & HIPEC), Disease free survival (DFS), Differentially methylated regions (DMR), Overall survival (OS), TableFormalin fixed paraffin embedded (FFPE), Hepatocellular carcinoma (HCC) ARTICLE CATEGORY: Research article NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice. 1 medRxiv preprint doi: https://doi.org/10.1101/2020.02.24.20027318; this version posted February 25, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license . NOVELTY AND IMPACT: Colorectal peritoneal metastasis (CPM) are associated with limited and variable survival despite patient selection using known prognostic factors and optimal currently available treatments.
    [Show full text]
  • Proteomic Analysis of the Marine Diatom Thalassiosira Pseudonana Upon Exposure to Benzo(A)Pyrene Raquel N Carvalho and Teresa Lettieri*
    Carvalho and Lettieri BMC Genomics 2011, 12:159 http://www.biomedcentral.com/1471-2164/12/159 RESEARCHARTICLE Open Access Proteomic analysis of the marine diatom Thalassiosira pseudonana upon exposure to benzo(a)pyrene Raquel N Carvalho and Teresa Lettieri* Abstract Background: Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants ubiquitously distributed. They are generated by incomplete combustion of organic materials such as wood or fossil fuels. Due to their carcinogenic, mutagenic effects and to their wide distribution in the environment, these pollutants pose many concerns to researchers and regulators. In our laboratories we investigated the effect of benzo(a)pyrene (BaP) exposure in the marine diatom Thalassiosira pseudonana, which has become an important model organism in aquatic toxicology studies. Results: In order to investigate the mechanism of action of PAHs, we exposed the diatoms for 24 h to 36.45 μg/L of BaP which inhibits the growth by about 30%, and analysed the relative protein expression profile by a quantitative proteomics approach based on iTRAQ labels. The proteomics profile analysis showed that around 10% of the identified proteins were regulated and one fourth of them confirmed the gene expression changes seen by DNA microarray. Particularly interesting was the down regulation of the Silicon transporter 1 (SIT1), an enzyme that is responsible for the uptake of silicon from the media into the diatom cells. Regulation of SIT1 upon BaP treatment was also confirmed at the gene expression level. Conclusions: The potential use of the regulated proteins found in this study as early indicators of environmental exposure to PAHs is discussed. In particular, SIT1 is considered a promising biomarker and SIT1 expression changes were confirmed also when the diatoms were exposed to field samples, e.g.
    [Show full text]
  • What Is Epigenetics? Two Views in Embryology
    What is Epigenetics? Two views in embryology Preformationism (17-19th century): each cell contains preformed elements that enlarge during development. Epigenesis (19th century -): chemical Humunculus reactions among soluble components in Hartsoecker 1695 the cell that execute a complex developmental plan. Chromosomes are Necessary for Development Before the 20th century Walter Sutton, 1902 Theodor Boveri, 1903 Columbia University University of Würzburg USA Germany • Determined that all chromosomes had to be present for proper embryonic development. • Development encoded by irreversible changes in chromosomes? Cell Specialization is Reversible Late 20th and early 21st centuries Skin cell nuclear transfers 99 Central Question: How can a single Original explant fertilized egg give rise to a complex removed Adult frog of \-nu strain Outgrowth of organism with cells of varied as nuclear donor epidermal cells phenotypes? Parent of 1st transfer Donor cells for recipient eggs Enucleation of nuclear transfer recipient eggs 1st nuclear transfer Cells trypsinized Foot web outgrowth and washed prove frog was 2-nu Uncleaved Completely cleaved (70 V) Martially cleaved /c o/\ • Gurdon, Laskey & Reeves 1975 (25%) demonstrated that “cell Dissociated cells for specialization does not involve serial transfer I *^i/ KJpZ* Parent of serial ttransfei r any loss, irreversible activation or Enucleation of 1 recipient eggs recipient eggs Serial nuclear transfer permanent change chromosomal Foot web outgrowth prove frog was 2-nu genes required for development” Uncleaved Completely cleaved (40/O Partially cleaved (30/0 (30%) Nuclear transplant tadpole: l-nu diploid from nucleolus and chromosome counts (present in 36% of serial clones) Fig. 2. Plan of serial nuclear transfer experiments, using nuclei from adult skin celJs.
    [Show full text]
  • Comparing the Effects of Vitamin E Tocotrienol-Rich Fraction Supplementation and Α-Tocopherol Supplementation on Gene Expression in Healthy Older Adults
    ORIGINAL ARTICLE Comparing the effects of vitamin E tocotrienol-rich fraction supplementation and α-tocopherol supplementation on gene expression in healthy older adults Siti Madiani Abdul Ghani,I Jo Aan Goon,I,* Nor Helwa Ezzah Nor Azman,I Siti Nor Asyikin Zakaria,I Zalina Hamid,II Wan Zurinah Wan NgahI I Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. II Sime Darby Foods & Beverages Marketing Sdn Bhd, Petaling Jaya, Selangor, Malaysia. Abdul Ghani SM, Goon JA, Nor Azman NHE, Asyikin Zakaria SN, Hamid Z, Wan Ngah WZ. Comparing the effects of Vitamin E Tocotrienol-Rich Fraction supplementation and α-Tocopherol supplementation on gene expression in healthy older adults. Clinics. 2019;74:e688 *Corresponding author. E-mail: [email protected] OBJECTIVES: This study aims to compare the differential gene expression resulting from tocotrienol-rich fraction and α-tocopherol supplementation in healthy older adults. METHODS: A total of 71 eligible subjects aged 50 to 55 years from Gombak and Kuala Lumpur, Malaysia, were divided into three groups and supplemented with placebo (n=23), α-tocopherol (n=24) or tocotrienol-rich fraction (n=24). Blood samples were collected at baseline and at 3 and 6 months of supplementation for microarray analysis. RESULTS: The number of genes altered by α-tocopherol was higher after 6 months (1,410) than after 3 months (273) of supplementation. α-Tocopherol altered the expression of more genes in males (952) than in females (731). Similarly, tocotrienol-rich fraction modulated the expression of more genes after 6 months (1,084) than after 3 months (596) and affected more genes in males (899) than in females (781).
    [Show full text]
  • Table S1. 103 Ferroptosis-Related Genes Retrieved from the Genecards
    Table S1. 103 ferroptosis-related genes retrieved from the GeneCards. Gene Symbol Description Category GPX4 Glutathione Peroxidase 4 Protein Coding AIFM2 Apoptosis Inducing Factor Mitochondria Associated 2 Protein Coding TP53 Tumor Protein P53 Protein Coding ACSL4 Acyl-CoA Synthetase Long Chain Family Member 4 Protein Coding SLC7A11 Solute Carrier Family 7 Member 11 Protein Coding VDAC2 Voltage Dependent Anion Channel 2 Protein Coding VDAC3 Voltage Dependent Anion Channel 3 Protein Coding ATG5 Autophagy Related 5 Protein Coding ATG7 Autophagy Related 7 Protein Coding NCOA4 Nuclear Receptor Coactivator 4 Protein Coding HMOX1 Heme Oxygenase 1 Protein Coding SLC3A2 Solute Carrier Family 3 Member 2 Protein Coding ALOX15 Arachidonate 15-Lipoxygenase Protein Coding BECN1 Beclin 1 Protein Coding PRKAA1 Protein Kinase AMP-Activated Catalytic Subunit Alpha 1 Protein Coding SAT1 Spermidine/Spermine N1-Acetyltransferase 1 Protein Coding NF2 Neurofibromin 2 Protein Coding YAP1 Yes1 Associated Transcriptional Regulator Protein Coding FTH1 Ferritin Heavy Chain 1 Protein Coding TF Transferrin Protein Coding TFRC Transferrin Receptor Protein Coding FTL Ferritin Light Chain Protein Coding CYBB Cytochrome B-245 Beta Chain Protein Coding GSS Glutathione Synthetase Protein Coding CP Ceruloplasmin Protein Coding PRNP Prion Protein Protein Coding SLC11A2 Solute Carrier Family 11 Member 2 Protein Coding SLC40A1 Solute Carrier Family 40 Member 1 Protein Coding STEAP3 STEAP3 Metalloreductase Protein Coding ACSL1 Acyl-CoA Synthetase Long Chain Family Member 1 Protein
    [Show full text]
  • Allele-Specific Genome Editing Using CRISPR-Cas9 Causes Off-Target
    bioRxiv preprint doi: https://doi.org/10.1101/397984; this version posted August 22, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Allele-specific genome editing using CRISPR-Cas9 causes off- 2 target mutations in diploid yeast 3 Arthur R. Gorter de Vries, Lucas G. F. Couwenberg, Marcel van den Broek, Pilar de la Torre Cortés, 4 Jolanda ter Horst, Jack T. Pronk and Jean-Marc G. Daran* 5 Department of Biotechnology, Delft University of Technology, Delft, 2629HZ, The Netherlands 6 * To whom correspondence should be addressed. Tel: 0031152782412; Email: [email protected] 7 ABSTRACT 8 Targeted DNA double-strand breaks (DSBs) with CRISPR-Cas9 have revolutionized genetic 9 modification by enabling efficient genome editing in a broad range of eukaryotic systems. Accurate 10 gene editing is possible with near-perfect efficiency in haploid or (predominantly) homozygous 11 genomes. However, genomes exhibiting polyploidy and/or high degrees of heterozygosity are less 12 amenable to genetic modification. Here, we report an up to 99-fold lower gene editing efficiency when 13 editing individual heterozygous loci in the yeast genome. Moreover, Cas9-mediated introduction of a 14 DSB resulted in large scale loss of heterozygosity affecting DNA regions up to 360 kb that resulted in 15 introduction of nearly 1700 off-target mutations, due to replacement of sequences on the targeted 16 chromosome by corresponding sequences from its non-targeted homolog. The observed patterns of 17 loss of heterozygosity were consistent with homology directed repair.
    [Show full text]
  • Systematic Analysis, Comparison, and Integration of Disease Based Human
    Zhang et al. BMC Medical Genomics 2010, 3:1 http://www.biomedcentral.com/1755-8794/3/1 RESEARCH ARTICLE Open Access Systematic analysis, comparison, and integration of disease based human genetic association data and mouse genetic phenotypic information Yonqing Zhang1†, Supriyo De1†, John R Garner1, Kirstin Smith1, S Alex Wang2, Kevin G Becker1* Abstract Background: The genetic contributions to human common disorders and mouse genetic models of disease are complex and often overlapping. In common human diseases, unlike classical Mendelian disorders, genetic factors generally have small effect sizes, are multifactorial, and are highly pleiotropic. Likewise, mouse genetic models of disease often have pleiotropic and overlapping phenotypes. Moreover, phenotypic descriptions in the literature in both human and mouse are often poorly characterized and difficult to compare directly. Methods: In this report, human genetic association results from the literature are summarized with regard to replication, disease phenotype, and gene specific results; and organized in the context of a systematic disease ontology. Similarly summarized mouse genetic disease models are organized within the Mammalian Phenotype ontology. Human and mouse disease and phenotype based gene sets are identified. These disease gene sets are then compared individually and in large groups through dendrogram analysis and hierarchical clustering analysis. Results: Human disease and mouse phenotype gene sets are shown to group into disease and phenotypically relevant groups at both a coarse and fine level based on gene sharing. Conclusion: This analysis provides a systematic and global perspective on the genetics of common human disease as compared to itself and in the context of mouse genetic models of disease.
    [Show full text]
  • Histone Chaperone Paralogs Have Redundant, Cooperative, and Divergent Functions in Yeast
    | INVESTIGATION Histone Chaperone Paralogs Have Redundant, Cooperative, and Divergent Functions in Yeast Neda Savic,* Shawn P. Shortill,* Misha Bilenky,† Joseph M. Dobbs,* David Dilworth,* Martin Hirst,† and Christopher J. Nelson*,1 *Department. Biochemistry and Microbiology, University of Victoria, BC V8W 3P6, Canada and †BC Cancer Agency Genome Sciences Centre and the Department of Microbiology & Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z3, Canada ORCID IDs: 0000-0001-8742-7442 (S.P.S.); 0000-0002-4123-2209 (J.M.D.) ABSTRACT Gene duplications increase organismal robustness by providing freedom for gene divergence or by increasing gene dosage. The yeast histone chaperones Fpr3 and Fpr4 are paralogs that can assemble nucleosomes in vitro; however, the genomic locations they target and their functional relationship is poorly understood. We refined the yeast synthetic genetic array approach to enable the functional dissection of gene paralogs. Applying this method to Fpr3 and Fpr4 uncovered redundant, cooperative, and divergent functions. While Fpr3 is uniquely involved in chromosome segregation, Fpr3 and Fpr4 cooperate to regulate genes involved in polyphosphate metabolism and ribosome biogenesis. We find that the TRAMP5 RNA exosome is critical for fitness in Dfpr3Dfpr4 yeast and leverage this information to identify an important role for Fpr4 at the 59 ends of protein coding genes. Additionally, Fpr4 and TRAMP5 negatively regulate RNAs from the nontranscribed spacers of ribosomal DNA. Yeast lacking Fpr3 and Fpr4 exhibit a genome instability phenotype at the ribosomal DNA, which implies that these histone chaperones regulate chromatin structure and DNA access at this location. Taken together. we provide genetic and transcriptomic evidence that Fpr3 and Fpr4 operate separately, cooperatively, and redundantly to regulate a variety of chromatin environments.
    [Show full text]
  • Biomedical Informatics
    BIOMEDICAL INFORMATICS Abstract GENE LIST AUTOMATICALLY DERIVED FOR YOU (GLAD4U): DERIVING AND PRIORITIZING GENE LISTS FROM PUBMED LITERATURE JEROME JOURQUIN Thesis under the direction of Professor Bing Zhang Answering questions such as ―Which genes are related to breast cancer?‖ usually requires retrieving relevant publications through the PubMed search engine, reading these publications, and manually creating gene lists. This process is both time-consuming and prone to errors. We report GLAD4U (Gene List Automatically Derived For You), a novel, free web-based gene retrieval and prioritization tool. The quality of gene lists created by GLAD4U for three Gene Ontology terms and three disease terms was assessed using ―gold standard‖ lists curated in public databases. We also compared the performance of GLAD4U with that of another gene prioritization software, EBIMed. GLAD4U has a high overall recall. Although precision is generally low, its prioritization methods successfully rank truly relevant genes at the top of generated lists to facilitate efficient browsing. GLAD4U is simple to use, and its interface can be found at: http://bioinfo.vanderbilt.edu/glad4u. Approved ___________________________________________ Date _____________ GENE LIST AUTOMATICALLY DERIVED FOR YOU (GLAD4U): DERIVING AND PRIORITIZING GENE LISTS FROM PUBMED LITERATURE By Jérôme Jourquin Thesis Submitted to the Faculty of the Graduate School of Vanderbilt University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE in Biomedical Informatics May, 2010 Nashville, Tennessee Approved: Professor Bing Zhang Professor Hua Xu Professor Daniel R. Masys ACKNOWLEDGEMENTS I would like to express profound gratitude to my advisor, Dr. Bing Zhang, for his invaluable support, supervision and suggestions throughout this research work.
    [Show full text]