Animal Cell Technology: Products from Cells, Cells
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ANIMAL CELL TECHNOLOGY: PRODUCTS FROM CELLS, CELLS AS PRODUCTS Animal Cell Technology: Products from Cells, Cells as Products Proceedings of the 16th ESACT Meeting April 25–29, 1999, Lugano, Switzerland Edited by A. Bernard B. Griffiths W. Noé F. Wurm KLUWER ACADEMIC PUBLISHERS NEW YORK, BOSTON, DORDRECHT, LONDON, MOSCOW eBook ISBN: 0-306-46875-1 Print ISBN: 0-792-36075-3 ©2002 Kluwer Academic Publishers New York, Boston, Dordrecht, London, Moscow All rights reserved No part of this eBook may be reproduced or transmitted in any form or by any means, electronic, mechanical, recording, or otherwise, without written consent from the Publisher Created in the United States of America Visit Kluwer Online at: http://www.kluweronline.com and Kluwer's eBookstore at: http://www.ebooks.kluweronline.com CONTENTS 16th ESACT Meeting Organising Committee xxi ESACT Executive Committee xxii Sponsors xxiii Companies Participating in the Trade Exhibition xxiv LIST OF PARTICIPANTS xxv INTRODUCTION Products from Cells - Cellas as Products lxxi ACKNOWLEDGEMENTS lxxiii CHAPTER I. Improvement and Induction of High Productivity Recombinant Pharmaceutical Protein Overexpression in an IRF-I Proliferation Controlled Production System Geserick, C.; Schroeder, K.; Bonarius, H.; Kongerslev, L.; Schlenke, P.; Hauser, H.; Mueller, P.P. 3 The Real Meaning of High Expression Grammatikos, S.I.; Bergemann, K.; Werz, W.; Brax, I.; Bux, R.; Eberhardt, P.; Fieder, J.; Noé, W. 11 A Novel Mammalian Gene Regulation System Responsive to Streptogramin Antibiotics Fussenegger, M.; Von Stockar, B.; Fux, C.; Rimann, R.; Morris, R.; Thompson, C.J.; Bailey, J.E. 19 Feedback Control of Redox Potential in Hybridoma Cell Culture Meneses, A.; Gomez, A.; Ramirez, O.T. 23 Genetic manipulation of the Protein Synthetic Capacity of Mammalian Cells Heaton, M.; Birch, J.; Hovey, A.; Kallmeier, R.; Proud, C.; James, D. 31 vi Retinoic Acid Enhances Monoclonal Antibody Production of Human-Human Hybridoma BD9 Inoue, Y.; Fujisawa, M.; Shoji, M.; Hashizume, S.; Katakura, Y.; Shirahata 35 Extracellular Adenosine 5’ Monophosphate Controls Proliferation and Stimulates Protein Production of rCHO Cells Bonarius, H.P.J.; Hansen, K.; Kongerslev, L. 39 The Mode of Actions of Lysozyme as an Immunoglobulin Production Stimulating Factor (IPSF) Sugahara, T.; Murakami, F.; Sasaki, T. 43 A Dissection of Recombinant Protein Expression in CHO Cells Indicating Protein-Specific, not Cell Specific, Limitation Altobello, K.; Chung, YH.; Iovino, J.; Luchette, C.; Overton, S.; Cunningham, M. 47 Effect of Bioreactor Process Control Parameters on Apoptosis and Monoclonal Antibody Production During the Protein-Free Fed-Batch Culture of a Murine Myeloma Moran, E.; McGowan, St.; Reynolds, N.; Wilson, C. 51 Adenovirus Vector Production in 293 Fed-Batch Cultures Wong, K.; Guardia, M.J.; Lee, St.; Hu, W.-S. 55 Influence of BCL-2 Over-Expression on NS0 and CHO Culture Viability and Chimeric Antibody Productivity Tey, B.T.; Singh, R.P.; Al-Rubeai, M. 59 A Regulatable Selective System Facilitating Isolation of High Expression Mammalian Cells Teruya, K.; Zhang, Y.-P.; Katakura, Y.; Shirahata, S. 63 Comparative Analysis of IRES Efficiency of Dicistronic Expression Vectorsi n Primary Cells and Permanent Cell Lines Schumacher, L.; Alves, P.M.; Wirth, M. 67 Use of Gel Microdrops Selection System for Recombinant Cells vii Hansen, K.; Bertelsen, C.; Kongerslev, L. 71 Bovine Serum-Albumin with Grafted RGD Tailed Cyclic Peptides as an Engineered Proadhesive Protein Dewelle, J.; Delforge, D.; Pirotton, S.; Remacle, J.; Raes, M. 75 Matrix Attachment Regions in Stable CHO Cell Line Development Zahn, M.M.; Kobr, M.; Mermod, N. 79 High Cell Density Cultivation of Hybridoma Cells Spin Filter vs Immobilized Culture Heine, H.; Biselli, M.; Wandrey, C. 83 Influence of the Inoculum on the Production of Antibodies with Mammalian Cells Inglin, M.; Graf-Hausner, U.; Sonnleitner, B.; Spinnler, R.; Leist, Ch. 87 Establishing and Developing CHO Cell Lines for the Commercial Production of Human Anti-Rhesus D IgG De Jesus, M.; Bourgeois, M.; Jordan, M.; Zahn, M.; Mermod, N.; Amstutz, H.P.; Wurm, F.M. 91 Glucose and Glutamine Replacement for the Enhancement of Fed-batch Proceses Using CHO Cells Altamirano, C.; Paredes, C.; Cairo, J.J.; Godia, F. 95 Pilot Scale Production of Human Prothrombin Using a Recombinant CHO Cell Line Lütkemeyer, D.; Heidemann, R.; Tebbe, H.; Gudermann, F.; Lehmann, J.; Schmidt, M.; Raditsch, M. 99 Maintenance of High Viability During Direct Transition of CHO-Kl Cell Growth from 10% to 0% Serum; No Adaptation Required Tsao, M.C.; Johnson, J.M.; Bachelder, C.; Wood, M.A.; Boline, J.A.; Berzofsky, R.N. 103 Analysis and Simultaneous Isolation of Recombinant t-PA Using High Performance Membrane Affinity Chromatography Renemann, G.; Kretzmer, G.; Tennikova, T.B. 107 viii CHAPTER II. Metabolic and Process Engineering Improvement of the Primary Metabolism of Cell Cultures Introducing a Pyruvate Carboxylase Reaction Pathway Irani, N.; Wirth, M.; V.d. Heuvel, J.; Beccaria, A.J.; Wagner, R. 113 Growth Kinetic Studies (In Hours) Based on the Fluorescence of Stable, GFP-Expressing CHO Cells Hunt, L.; Jordan H.; De Jesus, M.; Wurth, F.; 119 Intracellular Localization of Non-Secreted Recombinant -Trace Protein in Vesicle-Like Structures of Baculovirus-Infected Insect Cells Schulze, N.; Albers, U.; Grabenhorts, E.; Conradt, H.S.; Rohde, M., Nimtz, M.; Jäger 123 Induction Kinetics of Apoptosis in Mammalian Cell Cultures Linz, M.; Zeng, A.-P.; Deckwer, W.-D. 127 A Model for the On-Line Scrutiny of Metabolism: Its Application to the Changing Nutritional Demands of Cultured Animal Cells Guan, Y.H.; Kemp, R.B. 131 Caspases as a Target for Apoptosis Inhibition in Hybridoma Cells in Culture Gabernet, C.; Tinto, A.; Vives, J.; Prats, E.; Cornudella, L.; Cairo, J.J.; Godia, F. 135 Cell Activity after Cell Growth Inhibition by the IRF-1 System Coroadinha, A.S.; Carvalhal, A.V.; Moreira, J.L.; Carrondo, M.J.T. 139 Establishment of a Primary Liver Cell Culture from a Teleost, Oreochromis Mossambicus , the Tilapia: a Valid Tool for Physiological Studies Schmid, A.C.; Kloas, W.; Reinecke, M.; 143 TGB Induces Premature and Replicative Senescence in Cancer Cells ix Miura, T.; Katakura, Y.; Nakata, E.; Uehara, N.; Shirahata, S. 147 Development of a Fed-batch Strategy that Utilizes Multiple Carbon Sources to Maximize Recombinant Protein Expression in CHO Cells Deer, F., Cunningham, M.R. 151 Development of a New, Highly Flexible, Fully Computer Integrated Fermentor Control System Büntemeyer.; Nerkamp, M.; Bockholt, S.; Weber, C.; Gudermann, F.; Lehmann, J. 155 Cell Growth Rate Estimation in Packed-Bed and Hollow Fibers Bioreactors: Metabolism and Productivity Rodrigues, M.T.A.; Garbuio, A.; Nagao, L.T.; Raw, I.; Moro, A.M. 159 Influence of Oxygen Limitation on Myeloma Cell Cultures: Various Methods of Inoculum Proliferation for Bioreactor Cultivations Spinnler, R.; Graf-Hausner, U.; Inglin, M.; Leist, Ch. 163 Comparison of Fed-batch Strategies in Hybridoma Cultures Schwabe, J.O.; Wilkens, I.; Pöortner, R. 167 Non-Invasive Adaptive Control of the Feed Rate for High Cell Density CHO Cultures According to the On-line pO2 Profile Müller, D.; Doblhoff-Dier, O.; Katinger, H. 171 Cell Growth Control by the IRF-1 System in Perfusion Culture Carvalhal, A.V.; Moreira, J.L.; Carrondo, M.J.T. 175 Modulation of Cell Cycle for Optimal Recombinant Protein Production Hendrick, V.; Vandeputte, O.; Raschella, A.; Marique, T.; Cherlet, M.; Abdelkafi, C.; Wérenne, J. 179 Application of Bioassays for Suspended 293 Cell Enumerations in Chemically Defined Medium (CDM) Tsao, M.C.; Jacko, B.A.; Curnow, E.; Berzofsky, R.N. 183 X Efficient Cell Growth by an Optimised Protein Free Medium Design and a Controlled Feeding Strategy Loa, A.; Lefebvre, P.; Kretzmer, G. 187 Development of a Serum Free Medium for a Human Diploid Fibroblast Cell Line Pirotton, S.; Dewelle, J.; Eliaers, F.; Delforge, D.; Raes, M. 191 The Use of Peptones as Medium Additives for High-Density Perfusion Cultures of Animal Cells Heidemann, R.; Zhang, C.; Qi, H.; Rule, J.L.; Rozales, C.; Park, S.; Chuppa, S.; Ray, M.; Michaels, J.; Konstantinov, K.; Naveh, D.. 195 Determination of Animal Cell Densities and Viability by the New Analyser CEDEX2 When, T.; Lorenz, Th.; Behrendt, U.; Wallerius, C.; Büntemeyer, H.; Lehmann, J. 199 Determination of Animal Cell Densities and Viability; Comparison of the Microcyte Flow Cytometer with Trypan Blue Counts and Coulter Count Vriezen, N. 203 Mid-Infrared Spectroscopic Monitoring of Animal Cell Culture Bioractor Processes Rhiel, M.; Ziegler, T.; Ducommun, P.; Von Stockar, U.; Marison, I.W. 207 Mammalian Cell Monitoring using the Microcyte Flow Cytometer Harding, C.L.; Lloyd, D.r.; McFarlane, C.M.; Al-Rubeai, M. 211 Indirect Biomass Determination in Case of Non-Constant Metabolic Rates Ducommun, P., Ziegler, T.; Rhiel, M.; Von Stockar, U.; Marison, I.W. 215 Whole Cell – Smart Microsensor: A first Approach Towards Interfacing Live Cells and Electronics Charlier, O.; Goffaux, M.-J.; Marique, T.; Hendrick, V.; De Vos, L.; Werenne, J. 219 xi Comparison of Immobilised Group Specific Affinity Ligands for the Bioseparation of Antibodies by High Performance Membrane Affinity Chromatography Berruex, L.G.; Freitag, R. 223 CHAPTER III. Post-Translational Modifications Posttranslational Modifications Limit High Level Expression of Functionally Active Chimeric P-Selectin Glycoprotein Ligand-1 in rCHO Cells Sinacore, M.S.; Richards, T.; Francullo, L.; Woodard, A.; Hardy, M.; Cornell, R.; Koza, S.; Davies, M.; Ellis, D.; Harrison, S. 229 Glycolsylation Analysis of Nanomolar Amounts of Glycoprotein Comtining In-gel Enzymatic Digestion and FACE™ Kloth, C.; Leibiger, H.; Valley, U.; Yalcin, E.; Buchholz, R.; Emmrich, F.; Marx, U. 237 Glycosylation Patterns of a rec-Fusion Protein Expressed in BHK Cells at Different Metabolic States Cruz, H.J.; Conradt, H.S.; Peixoto, C.M.; Alves, P.M.; Nimtz, M.; Dias, E.M.; Santos, H.; Moreira, J.L.; Carrondo, M.J.T. 241 Elimination of N-Glycolylneuraminic Acid Residues in Recombinant Glycoproteins: A Gene Knock-Out Approach Fontana, O.; Covini, N.; Pighini, M.; Carpani, D.; Buono, P.; Soria, M.R.; Monaco, L.