CV - Line Hjort, PhD., MSc.

Værnedamsvej 4A, 3. th. • 1619 København V • [email protected] • 25 78 41 08 From Assens, Fyn, Birthdate: 30.06.1985

Education

Mar 2018: PhD degree: Project title: “Diabetes in pregnancy and epigenetic mechanisms”, 2nd March 2018, Faculty of Health and Medical Sciences, Copenhagen University. Dec 2011: MSc. Human Biology. Faculty of Health and Medical Sciences, Copenhagen University. Jun 2008: BSc. Biology. Faculty of Natural Sciences, Copenhagen University.

Employments

Jan 2019 – : Postdoc, Dept. of Obstetrics, Rigshospitalet, Copenhagen, Denmark. Research of diabetes and obesity in pregnancy and its impact on offspring DNA and later health. Working with international collaborators on European, LMICs and Australian birth cohort studies.

May 2018 – Jan 2019: Project manager, Sens Innovations, Copenhagen, Denmark. Research consultant at biotech company producing activity sensors for clinical studies.

Aug – Oct 2018 -: Research fellow, The and Diabetes Research Group, Clinical Research Center, Lund University, Sweden. Performing epigenetic studies in human and mouse muscle tissue/stem cell cultures.

Sep 2013 – Mar 2018: PhD student, Dept. of Endocrinology, Rigshospitalet, Denmark. - Other work responsibilities besides PhD studies: Project collaborator of Tanzanian study of anemia during pregnancy, and project assistant of Indian-Danish collaboration study in pregnancy diabetes research.

Sep 2014 – May 2015: Visiting PhD student, The Cancer and Disease Epigenetics Group, Murdoch Children’s Research institute, Melbourne, Australia. Temporarily stationed at a top-experienced epigenetic laboratory, conducting epigenetic experiments and bioinformatic training including analysis of microarray data.

2011 – 2013: Research assistant, Dept. of Endocrinology, Rigshospitalet, Denmark.

- Project assistant of a NIH-funded pregnancy diabetes study in 2400 mothers and children, including performing clinical examinations and sample processing. Responsible for the biospecimen procedures. - Performing epigenetic studies in human muscle and adipose tissue/stem cell cultures. - Writing international/national grant applications. Biology

2010 – 2011: Master thesis student, Steno Diabetes Center/ Dept. Endocrinology, Rigshospitalet, Denmark, and The Epigenetics and Diabetes Research Group, Clinical Research Center, Lund University, Sweden.

Human MSc.

Scientific presentations and teaching ., PhD Presentations: From 2012-2019, more than 45 oral- and poster presentations at international and national , conferences/meetings, including:

- Apr 2019: Invited speaker at the Medical University of Graz, Austria, for one-day symposium. Line Hjort Line

- Sep 2018: Oral presentation at the 50th Diabetic Pregnancy Study Group Meeting (DPSG), Rome. – Recipient of Young Investigator Award. - Sep 2017: Oral presentation at the 49th DPSG Meeting, Nyborg. Recipient of Young Investigator Award. - Nov 2016: Oral presentation at the 3rd BBDC-Joslin-UCPH Conference, Harvard Medical School, Boston. Recipient of Travel grant award. - May 2014: Poster presentation. Annual PhD day, Faculty of Health and Medical Sciences, Copenhagen

University, Copenhagen. Winner of best poster presentation. Vitae Curriculum 1

Teaching: - Mar 2012 – : Supervising student assistants, bachelor- and master students. - Sep 2018 and 2019: Lecturer in basic genetics, epigenetics and fetal development at the course “Ethnicity and Cardiometabolic Diseases in a Global Perspective” at Dept. of Global Health, Copenhagen University. - Sep-Dec 2015 and 2016: Teaching at Nørre Gymnasium and Christianshavn´s Gymnasium, as part of research internship project for high school students.

Mobility and international experience

- Visiting PhD stud. (2014-2015) and planned visit as postdoc (Oct-Nov 2019) in Prof. Richard Saffery´s lab, Epigenetics group, Murdoch Children’s Research institute, Melbourne, Australia (ongoing collaboration). - Collaborator of a Tanzanian study of anemia and malaria during pregnancy, responsible of writing protocols for sampling of biospecimens and implementing these locally in Tanzania, at the Magunga Hospital, Korogwe, Tanga region. - Project assistant of Indian-Danish collaboration study in pregnancy diabetes research, including training local researchers in DNA techniques, at the Centre of Cellular and Molecular Biology in Hyderabad. - In total ~12 months’ full-time work in Prof. Charlotte Ling´s lab “Diabetes and Epigenetics”, Lund Universi- ty Diabetes Centre, Malmö, Sweden, from 2013 (ongoing collaboration).

Other ongoing national and international collaborations

- Prof. Ib Bygbjerg and Postdoc Christentze Schmiegelow, Dept of Global health, Copenhagen University, (Tanzanian and other LMIC studies). - Prof. Louise Dalgaard and Postdoc Anja Sørensen, Centre for Frustrated Molecular Interactions, Roskilde University, Denmark (Diabetes in pregnancy and epigenetic studies). - Prof. Gernot Desoye, Dept. of Obstetrics and Gynecology, Medical University of Graz, Austria (EU-funded project (the DALI study) on obesity and diabetes in pregnancy and placenta biopsies). - Dignity (Danish center for Torture victims) and the Kosovo Rehabilitation Center for Torture victims, Pristina, Kosovo (Study of epigenetic changes in offspring of women with PTSD from the war in Kosovo). - Prof. Cuilin Zhang, National Institute of Child Health and Human Development, NIH, Bethesda, MD, USA ( study in >10.000 Danish and American mothers and children). - Several ongoing projects with Prof. Peter Damm, Postdoc Louise Groth Grunnet and colleagues at Dept.s of Obstetrics and Endocrinology, Rigshospitalet, Copenhagen, and VP. Allan Vaag, AstraZeneca, Sweden.

Institutional responsibilities and project management

- 2013– : Lab manager of the biospecimen processing and nucleic acid extractions laboratory, Dept. of

Endocrinology, The Diabetes and Bone-metabolic Research Unit, Rigshospitalet, Copenhagen. Biology - 2015– : Responsible for the biobank/freezer stall at Dept. of Endocrinology, The Diabetes and Bone- metabolic Research Unit, Rigshospitalet, Copenhagen. - Apr 2019: Postdoctoral Research Leadership Course, CBS executive, Copenhagen.

MSc. Human Human MSc.

Miscellaneous .,

PhD , , • Received grants: Dec 2018: postdoc grant from the Danish Diabetes Academy (DDA). May and July 2013: PhD grants from the DDA, and the Faculty of Health and Medical Sciences, Copenhagen University. In

2018-2019: research grants to cover project expenses, from Danish foundations (Frode Nygård´s Fond,

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A.P. Møller Lægefonden, Leo Nielsen og Hustru´s Legat, Lægeforeningens Forskningsfond). – • Conference-manager and secretary of the meeting: “Diabetes and obesity – in a global life course perspective Indo-Danish research collaboration”, managed by Innovationsfonden, 4-5th Sep 2014, Cph. • Reviewer for several journals including “Diabetes” and “Clinical Epigenetics”, as well as EU-grant proposal. • Member of FYF, the “Union of Young Researchers”, Copenhagen University Hospital. • Member of the Danish Diabetes Academy. • Biked to Paris with team Rynkeby Rigshospitalet in 2017. Vitae Curriculum 2

Publication list - Line Hjort, PhD., MSc.

Værnedamsvej 4A, 3. th. • 1619 København V • [email protected] • 25 78 41 08

Bibliometric summary (Web of Science, 01.07.2019)

First authorships: 8 published /accepted for publication Co-authorships: 14 published Book chapters: 2 H-index: 7, Number of citations: 156, ORCID ID: http://orcid.org/0000-0003-4673-4176

Original peer-reviewed publications

Impact factor (IF, 2-years): based on the 2018 Scimago Journal and Country Rank (SJR).

1. Broholm C*, Ribel-Madsen R*, Hjort L*, Olsson A, Ahlers J, Hansen NS, Schrölkamp M, Gillberg L, Perfilyev A, Volkov P, Ling C, Jørgensen S, Mortensen B, Hingst J, Wojtaszewski J, Scheele C, Brøns C, Pedersen BK, Vaag A. Epigenome- and transcriptome-wide changes in muscle stem cells from low birth weight men. *Equal contribution. Endocrine Research, 2019 Sep. doi: 10.1080/07435800.2019.1669160. (IF: 1.52).

2. Fernandez-Twinn DS*, Hjort L*, Novakovic B*, Ozanne SE, Saffery R. Intrauterine programming of obesity and . *Equal contribution. Diabetologia 2019 Aug 27. doi: 10.1007/s00125-019-4951-9. (IF: 7.11).

3. Gillberg L, Rönn T, Jørgensen SW, Perfilyev A, Hjort L, Nilsson E, Brøns C, Vaag A, Ling C. Fasting unmasks differential fat and muscle transcriptional regulation of metabolic gene sets in low versus normal birth weight men. EBioMedicine. 2019 Aug 19. pii: S2352-3964(19)30533-X. (IF: 6.49).

4. Hjort L, Novakovic B, Grunnet LG, Maple-Brown L, Damm P, Desoye G, Saffery R. Diabetes in pregnancy and epigenetic mechanisms – How the first 9 months from conception may affect the child´s epigenome and later risk of disease. Lancet Diabetes and Endocrinol. 2019 May 22. pii: S2213-8587(19)30078-6. (IF: 23.71).

5. Hjort L, Møller SL, Minja DTR, Msemo OA, Nielsen BB, Christensen DL, Theander TG, Nielsen K, Larsen LG, Grunnet LG, Groop L, Prasad RB, Lusingu JPA, Schmiegelow C, Bygbjerg IC. Cohort profile: FETALforNCD - Fetal exposure and Epidemiological Transition: the role of Anemia in early Life for Non-Communicable Diseases in later life - A prospective preconception study in rural Tanzania. BMJ open. 2019 May 22;9(5):e024861. doi: 10.1136/bmjopen-2018-024861. (IF: 2.42).

6. Grunnet LG, Pilgaard K, Alibegovic A, Jensen CB, Hjort L, Ozanne SE, Bennett M, Vaag A, Brøns C. Biology Human MSc.

Leukocyte telomere length is associated with elevated plasma glucose and HbA1c in young healthy men .,

independent of birth weight. PhD Sci Rep. 2019 May 21;9(1):7639. doi: 10.1038/s41598-019-43387-0. (IF: 4.12). ,

7. Kampmann FB, Thuesen ACB, Hjort L, Olsen SF, Pires SM, Tetens I, Grunnet LG. Line Hjort Line

Exposure to gestational diabetes is a stronger predictor of dysmetabolic traits in children than size at birth.

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J Clin Endocrinol Metab. 2018 Dec 5. doi: 10.1210/jc.2018-02044. (IF: 4.73). st

8. Maslova E, Hansen S, Grunnet LG, Strøm M, Bjerregaard AA, Hjort L, Kampmann FB, Madsen CM, Thuesen ACB, Bech BH, Halldorsson TI, Vaag AA, Zhang C, Olsen SF.

Maternal glycemic index and glycemic load in pregnancy and offspring metabolic health in childhood and Publication li Publication adolescence-a cohort study of 68,471 mother-offspring dyads from the Danish National Birth Cohort. 1

Eur J Clin Nutr. 2018 Sep 24. doi: 10.1038/s41430-018-0316-6. (IF: 3.04).

9. Hjort L, Martino D, Grunnet LG, Naeem H, Maksimovic J, Olsson A, Zhang C, Ling C, Olsen SF, Saffery R, Vaag A. Gestational diabetes and maternal obesity are associated with epigenome-wide methylation changes in children. JCI Insight. 2018 Sep 6;3(17). pii: 122572. (IF: 6.01).

10. Houshmand-Oeregaard A, Schrölkamp M, Kelstrup L, Hansen NS, Hjort L, Thuesen ACB, Broholm C, Mathiesen ER, Clausen TD, Vaag A, Damm P. Increased expression of microRNA-15a and microRNA-15b in skeletal muscle from adult offspring of women with diabetes in pregnancy. Hum Mol Genet. 2018 Mar 8. DOI: 10.1093/hmg/ddy085. (IF: 4.48).

11. Ström K, Morales-Alamo D, Ottosson F, Edlund A, Hjort L, Jörgensen SW, Almgren P, Zhou Y, Martin-Rincon M, Ekman C, Pérez-López A, Ekström O, Perez-Suarez I, Mattiasson M, de Pablos-Velasco P, Oskolkov N, Ahlqvist E, Wierup N, Eliasson L, Vaag A, Groop L, Stenkula KG, Fernandez C, Calbet JAL, Holmberg HC, Hansson O. N1-methylnicotinamide is a signalling molecule produced in skeletal muscle coordinating energy metabolism. Sci Rep. 2018 Feb 14;8(1):3016. (IF: 4.12).

12. Hjort L, Vryer R, Grunnet LG, Burgner D, Zhang C, Olsen SF, Saffery R, Vaag AA. Shorter telomere length in 9- to 16-year-old children exposed to Gestational Diabetes in utero. Diabetologia 2018 Apr;61(4):870-880. (IF: 7.11).

13. Houshmand-Oeregaard A, Hjort L, Kelstrup L, Hansen NS, Broholm C, Gillberg L, Clausen TD, Mathiesen ER, Damm P, Vaag A. DNA methylation and gene expression of TXNIP in adult offspring of women with diabetes in pregnancy. PLoS One. 2017 Oct 27. DOI: 10.1371/journal.pone.0187038. (IF: 2.88).

14. Grunnet LG, Hansen S, Hjort L, Madsen CM, Kampmann FB, Thuesen ACB, Granstrøm C, Strøm M, Maslova E, Frikke-Schmidt R, Damm P, Chavarro JE, Hu FB, Olsen SF, Vaag A. Adiposity, Dysmetabolic Traits and Earlier Onset of Female Puberty in Adolescent Offspring of Women With Gestational Diabetes Mellitus: A Clinical Study Within the Danish National Birth Cohort. Diabetes Care. 2017 Oct 16. DOI: 10.2337/dc17-0514. (IF: 13.10).

15. Maslova E, Hansen S, Grunnet LG, Strøm M, Bjerregaard AA, Hjort L, Kampmann FB, Madsen CM, Baun Thuesen AC, Bech BH, Halldorsson TI, Vaag AA, Olsen SF. Maternal protein intake in pregnancy and offspring metabolic health at age 9-16 y: results from a Danish cohort of gestational diabetes mellitus pregnancies and controls. Am J Clin Nutr. 2017 Aug;106(2):623-636. (IF: 6.66).

16. Hjort L, Jørgensen SW, Gillberg L, Hall E, Brøns C, Frystyk J, Vaag AA, Ling C. 36 h fasting of young men influences adipose tissue DNA methylation of LEP and ADIPOQ in a birth weight-

dependent manner. MSc. Human Biology Human MSc.

Clin Epigenetics. 2017 Apr 21;9:40. (IF: 5.43). ., PhD

17. Houshmand-O. A, Hansen NS, Hjort L, Kelstrup L, Broholm C, Mathiesen ER, Clausen TD, Damm P, Vaag A. , Differential adipokine DNA methylation and gene expression in subcutaneous adipose tissue from adult offspring of women with diabetes in pregnancy.

Clin Epigenetics. 2017 Apr 13;9:37. (IF: 5.43).

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18. Hansen NS, Strasko KS, Hjort L, Kelstrup L, Houshmand-Øregaard A, Schrölkamp M, Schultz HS, Scheele C, st Pedersen BK, Ling C, Clausen TD, Damm P, Vaag A, Broholm C. Fetal hyperglycemia changes human preadipocyte function in adult life.

J Clin Endocrinol Metab. 2017 Apr 1;102(4):1141-1150. (IF: 4.73). Publication li Publication

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19. Kelstrup L*, Hjort L*, Houshmand-Oeregaard A, Clausen TD, Hansen NS, Broholm C, Borch-Johnsen L, Mathiesen ER, Vaag AA, Damm P. Gene expression and DNA methylation of PPARGC1A in Muscle and Adipose Tissue from Adult Offspring of Women with Diabetes in Pregnancy. Diabetes. 2016 Oct;65(10):2900-10. *Equal contribution. (IF: 6.73).

20. Hansen NS, Hjort L, Broholm C, Gillberg L, Schrölkamp M, Schultz HS, Mortensen B, Jørgensen SW, Friedrichsen M, Wojtaszewski JF, Pedersen BK, Vaag A. Metabolic and Transcriptional Changes in Cultured Muscle Stem Cells from Low Birth Weight Subjects. J Clin Endocrinol Metab. 2016 May;101(5):2254-64. (IF: 4.73).

21. Jørgensen SW, Brøns C, Bluck L, Hjort L, Færch K, Thankamony A, Gillberg L, Friedrichsen M, Dunger DB, Vaag AA. Metabolic response to 36 hours of fasting in young men born small vs appropriate for gestational age. Diabetologia. 2015 Jan;58(1):178-87. (IF: 7.11).

22. Vaag A, Brøns C, Gillberg L, Hansen NS, Hjort L, Arora GP, Thomas N, Broholm C, Ribel-Madsen R, Grunnet LG. Genetic, nongenetic and epigenetic risk determinants in developmental programming of type 2 diabetes. Acta Obstet Gynecol Scand. 2014 Nov;93(11):1099-108. (IF: 2.80).

Other publications

23. Kelstrup L, Bytoft B, Hjort L, Houshmand-Oeregaard A, Mathiesen ER, Damm P, Clausen TD. Chapter: Diabetes in pregnancy – Long term complications of offspring. “Diabetes in pregnancy – an overview”. Book chapter accepted for publication Aug 2019, in press.

24. Gillberg L, Hjort L. Chapter 37: Epigenetics of Metabolic Diseases. “Handbook of Epigenetics”. December 2017. DOI: 10.1016/b978-0-12-805388-1.00037-7.

MSc. Human Biology Human MSc.

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