Unusual Pungency from Extra-Virgin Olive Oil Is Attributable to Restricted Spatial Expression of the Receptor of Oleocanthal
The Journal of Neuroscience, January 19, 2011 • 31(3):999–1009 • 999 Behavioral/Systems/Cognitive Unusual Pungency from Extra-Virgin Olive Oil Is Attributable to Restricted Spatial Expression of the Receptor of Oleocanthal Catherine Peyrot des Gachons,1 Kunitoshi Uchida,3,4 Bruce Bryant,1 Asako Shima,3 Jeffrey B. Sperry,2 Luba Dankulich-Nagrudny,1 Makoto Tominaga,3,4 Amos B. Smith III,1,2 Gary K. Beauchamp,1 and Paul A. S. Breslin1,5 1Monell Chemical Senses Center and 2Department of Chemistry, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, 3Okazaki Institute for Integrative Bioscience, Okazaki 444-8787, Japan, 4Department of Physiological Sciences, Graduate University for Advanced Studies, Okazaki 444-8585, Japan, and 5Department of Nutritional Sciences, Rutgers University School of Environmental and Biological Sciences, New Brunswick, New Jersey 08901 Oleocanthal, a major phenolic compound in extra-virgin olive oil with antiinflammatory properties, elicits an unusual oral pungency sensed almost exclusively in the throat. This contrasts with most other common oral irritants, such as cinnamaldehyde, capsaicin, and alcohol, which irritate mucus membranes throughout the oral cavity. Here, we show that this rare irritation pattern is a consequence of both the specificity of oleocanthal for a single sensory receptor and the anatomical restriction of this sensory receptor to the pharynx, within the oral cavity. We demonstrate, in vitro, that oleocanthal selectively activates the hTRPA1 channel in HEK 293 cells and that its ability to excite the trigeminal nervous system in rodents requires a functional TRPA1. Moreover, we similarly demonstrate that the over-the-counter analgesic, ibuprofen, which elicits the same restricted pharyngeal irritation as oleocanthal, also specifically excites rodent sensory neurons via TRPA1.
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