Phylogeny of Hawaiian Melicope (Rutaceae): RAD-Seq Resolves Species Relationships and Reveals Ancient Introgression
ORIGINAL RESEARCH published: 17 September 2019 doi: 10.3389/fpls.2019.01074 Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression Claudia Paetzold 1*, Kenneth R. Wood 2, Deren A. R. Eaton 3,4, Warren L. Wagner 5 and Marc S. Appelhans 1,5 1 Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium), University of Göttingen, Goettingen, Germany, 2 National Tropical Botanical Garden, Kalaheo, HI, United States, 3 Department of Ecology, Evolution and Environmental Biology, Columbia University, New York, NY, USA, 4 Department of Ecology, Evolution, and Environmental Biology, Columbia University, New York, NY, United States, 5 Department of Botany, Smithsonian Institution, Washington, DC, United States Hawaiian Melicope are one of the major adaptive radiations of the Hawaiian Islands comprising 54 endemic species. The lineage is monophyletic with an estimated crown age predating the rise of the current high islands. Phylogenetic inference based on Sanger sequencing has not been sufficient to resolve species or deeper Edited by: level relationships. Here, we apply restriction site-associated DNA sequencing (RAD- Gonzalo Nieto Feliner, Real Jardín Botánico (RJB), seq) to the lineage to infer phylogenetic relationships. We employ Quartet Sampling to Spain assess information content and statistical support, and to quantify discordance as well Reviewed by: as partitioned ABBA-BABA tests to uncover evidence of introgression. Our new results Ya Yang, University of Minnesota drastically improved resolution of relationships within Hawaiian Melicope. The lineage is Twin Cities, United States divided into five fully supported main clades, two of which correspond to morphologically Mario Fernández-Mazuecos, circumscribed infrageneric groups.
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