Some Insect Pests of Horses and Mules

Total Page:16

File Type:pdf, Size:1020Kb

Some Insect Pests of Horses and Mules Some Insect Pests of Horses and Mules BY F. C. BISHOPP ^ HERE IS condensed and practical information about several enemies of the horse and mule, including mos- quitoes^ sand flies (punkies), houseflies, horseflies, deer flies, buffalo gnats (blackflies), fleas, lice, and ticks. Special attention is given to control measures. A CONSIDERABLE KUMiiER of iiisccts and inscct relatives attack equines in addition to the horse bots, stableflies, ticks^ screwworms, and mange mites discussed in other articles in this book. Some of them, such as the biting and sucking lice of horses and mules, are specific; some, like the tropical horse tick, attack other animals but prefer to feed on equines; and many others feed on various warm-blooded animals with- out seeming to prefer any one kind. A number of such pests at times become so abundant locally as to have serious effects on horses, causing poor condition, lower work output, difliculty in handling, and even death. MOSQUITOES AND SAND FLIES Many kinds of mosquitoes and sand flies, or punkies, attack horses and nmles. As a rule, a moderate number of these insects do not greatly annoy horses; but the presence of large numbers sometimes constitutes a serious problem, especially amon^ high-strung animals such as race horses. Difficulties due to these insects have been en- countered at meets and fairs held during the late spring and early fall in cities along the south Atlantic coast. Screening the stables gives some protection against mosquitoes. Sand flies, however, readily pass through ordinary screens, and smudges and sprays are resorted to for relief from them. Painting the screens in the evening with a mixture of 1 part pyrethrnm extract 1 F. C. Bishopp i« Assistant Chief, Bureau of Entomology and Plant Quarantine. .492 Some Insect Pests of Horses and Mules 493 concentrate and 20 parts lubricating oil (S. A. E. 5), together with the use of a pyrethrum-kerosene spray in the buildings, is effective.^ Pasturing the horses on high ground free from dense woods and underbrush reduces the mosquito and sand fly hazards outdoors. Eliminating stagnant water in and near pastures, emptying and cleaning water troughs frequently, and, in salt marsh areas, ditch- ing the parts of the marsh known to produce mosquitoes are effective steps in preventing trouble. Since there are many species of mos- quitoes and they have widely divergent habits, control measures must be modified to fit the situation. After stagnant water has been elimi- nated as far as practicable, the principal lines of attack are: (1) Apply No. 2 fuel oil lightly to the surface pools where mosquito wig- glers are found; (2) keep livestock on high, open pastures when mosquitoes are abundant; (3) keep valuable animals in screened barns; (4) use kerosene-pyrethrum sprays to kill the mosquitoes on animals and in the barns; and (5) make smudges in which stock may find' protection during severe mosquito outbreaks. In this brief account it is not possible to discuss all the different methods of controlling mosquitoes. The reader is therefore referred to other published articles that supply more details (i, 4? '^)-^ The fact should not be lost sight of that at least eight American species of mosquitoes have been shown to be capable of carrying equine encephalomyelitis. Epizootics, or severe outbreaks, of this disease are usually concurrent with mosquito outbreaks, and pro- tecting animals f nom mosquito attack appears to be of some value in preventing the disease. THE HOUSEFLY The housefly is not of great importance as an enemy of the horse, but, if not controlled, it breeds in tremendous numbers in horse manure. The presence of this insect in abundance is a serious hazard to human health. The housefly annoys horses by its persistent efforts to feed on body secretions, particularly those of the eyes. This leads to the suspicion that the flies may transmit certain infectious eye diseases. The house- fly is an intermediate host and transmitter of the roundworm para- site of equines, Habronema muscae (8), and it has also been shown to be capable, of. transmitting mastitis of cattle. The control of houseflies depends on the proper disposal of manure and vegetable refuse (S). Scattering such materials thinly over fields soon to be plowed is the most satisfactory method of handling them. Manure may be stored in fly-tight manure pits or on racks over water. Treating manure with borax at the rate of 11 ounces to each 8 bushels also checks fly breeding. If the manure is to be used as a fertilizer, borax should not be used in excess of this amount. Care should be exercised not to apply too large an amount of treated manure to soil, since applications heavier than 15 tons per acre are likely to injure some crops on certain soil types. 2 UNITED STATES BUREAU OF ENTOMOLOGY AND PLANT QUAEANïINE. SAND FLIES AND PUNKiEs. U. S. Bur. Ent. and Plant Quar. Cîr. E-441, 3 pp. 1938. [Processed.] = Italic numbers in parentheses refer to Literature Cited, p. 499. 494 Yearbook of Agriculture, 1942 Tlic use of properly bnitod fly trii])s as well as of keroseiie- pyrethruin fly sprays is an important siipplomontal control measure. Instructions for makino; a tlioroujihly practical fly trap will be fonnd in Farmers" Bulletin 734. Flytraps and Their Operation. HORSEFLIES AND DEERFLIES The blood-sucking insects known as Iiorsetlics and deer flies are often very troublesome to horses. Their bites are painful, and when the insects are numerous, they draw considerable blood. They also act as carriers of anthrax, the Asiatic disease surra, and possibly other equine maladies. There are many kinds of horseflies and deer flies of various sizes and colors (fig. 1). Some are relatively small, not more than half an inch in length, while otiicrs are three times that size and very lobust. These pests are widely distributed in this coun- trj\ being found in greatest numbers in marshy or swampy areas and along streams, where they breed. The eggs are laid on plants FiCURE 1.- -A species of horselly. Three times iialiiral growing in or over the size. water or on stones in . streams. xVfter hatch- ing, the larvae drop into the water and buirow into the imid or gravel, wiiere they feed on small, soft-bodied animals. After nearly a year they pupate in the moist soil near the water, and soon the adult in- sects emerge. The adults are strong, fast fliers and may be found several miles away from water, but they are usually more abundant near it. As the breeding habits suggest, control is very difficult. Draining swamps and seepage areas along streams, deepening and straighten- ing the edges of lakes and ponds, and preventing accumulations of waste irrigation water in low places in meadows tend to reduce the mimber of horseflies. Wlien these pests are abundant, animals in harness may be given some relief by the use of burlap coverings. Darkened sheds and smudges ofl'er some protection to livestock not being worked. Horse- flies are not easily rei)elled, and as the individual flies usually remain on the animals for only very short periods, fly sprays are of little -value. BUFFALO GNATS Buffalo gnats, or blackflies, are pests of nearly all warm-blooded animals, but certain species prefer certain hosts. They are small, Some Insect Pests of Horses and Mules 495 rather heavy-set, humpbacked flies, or gnats (fig, 2). The bites they inflict on human beings are often very irritating; when there are many bites, the face and arms may become swollen, and severe generalized symptoms may result. Livestock are greatly annoyed by the bites of these insects. In severe outbreaks of the southern buffalo gnat in the lower Mississippi Valley many mules die, cattle nnd liorses are reduced in flesh, milk flow is cut, and the coats of the animals become rough and unsightly. Buffalo gnats breed only in running water. The larvae attach themselves to sticks, logs, stones, and aquatic plants in the faster flowing parts of streams. Stones or dams over w^iich water is rush- ing are often literally covered with the larvae, which, when fuU- o'rown, are from one-third to one-half inch in length. The larvae usually require sev- eral months to develop; then they pupate in cornucopialike structures which they spin on rocks in the water. The gnats burst out of the pupa skins and in some way reach the surface of the rough water and fly off. The adult females appear to require one or more meals of blood before eggs develop. These are laid in a gelatinous mass on stones or twigs at the water's surface or, as in the case of the southern buffalo gnat, are dropped singly on the water as the females di]) to the surface from the swarm of gnats dancing over it. Bradley (2) has found that the eggs of thé southern buffalo gnat sink to the bottom and do not hatch until the following FIGURE 2.—Adult buffalo s])ring when floods occur again. The eggs gnat, or blackfly. Much of most species hatch in a few days. enlarged. The southern buffalo gnat usually appears in swarms in the lower Mississippi Valley during the first warm period of late winter or early spring. If the floods are widespread and the weather continues warm, tremendous numbers of gnats emerge. After a few weeks the gnats disappear, sometimes very suddenly. The turkey gnat {EiisimuMmn méridionale)^ which usually appears later in the spring than does the southern buffalo gnat, attacks live- stock to a limited extent but is especially destructive to poultry. Preventing the breeding of the southern buffalo gnat is very dif- ficult.
Recommended publications
  • Backyard Mosquito Management Practices That Do Not Poison You Or the Environment by Becky Crouse
    A BEYOND PESTlClDES FACT SHEET O A BEYOND PESTlClDES FACT SHEET O A BEYOND PESTlClDES FACT SHEET Backyard Mosquito Management Practices that do not poison you or the environment By Becky Crouse irst and foremost, let’s get a couple of things straight. (1995), pgs. 17-29). He has continually questioned the effi- Mosquito management does NOT mean dousing your- cacy of spray-based strategies against mosquitoes, conduct- Fself and your kin in your favorite DEET product and ing research for several cities in the mid-1980s. then stepping out to enjoy the local wildlife. It is not swat- ting at the suckers as they bite you. And it is not investing in Life Cycle of a Skeeter one of those full-body net suits for your next camping trip. To manage mosquitoes, you have to get rid of the situa- There are more than 2,500 different species of mosquitoes in the tions that are attracting them to your world, 150 of which occur in the U.S. property, and, if you detect any and a only small fraction of which ac- breeding activity, kill them before tually transmit disease. they become adults. That’s called Mosquitoes go through four LARVACIDE! stages in their life cycle – egg, larva, So what then do mosquitoes pupa, and adult. Eggs can be laid ei- need? Why are they finding your ther one at a time or in rafts and float backyard so darn attractive? They on the surface of the water. Culex and need suitable aquatic breeding habi- Culiseta species stick their eggs to- tats in order to complete their life gether in rafts of 200 or more, which cycle (a.k.a they need water).
    [Show full text]
  • Insects Commonly Mistaken for Mosquitoes
    Mosquito Proboscis (Figure 1) THE MOSQUITO LIFE CYCLE ABOUT CONTRA COSTA INSECTS Mosquitoes have four distinct developmental stages: MOSQUITO & VECTOR egg, larva, pupa and adult. The average time a mosquito takes to go from egg to adult is five to CONTROL DISTRICT COMMONLY Photo by Sean McCann by Photo seven days. Mosquitoes require water to complete Protecting Public Health Since 1927 their life cycle. Prevent mosquitoes from breeding by Early in the 1900s, Northern California suffered MISTAKEN FOR eliminating or managing standing water. through epidemics of encephalitis and malaria, and severe outbreaks of saltwater mosquitoes. At times, MOSQUITOES EGG RAFT parts of Contra Costa County were considered Most mosquitoes lay egg rafts uninhabitable resulting in the closure of waterfront that float on the water. Each areas and schools during peak mosquito seasons. raft contains up to 200 eggs. Recreational areas were abandoned and Realtors had trouble selling homes. The general economy Within a few days the eggs suffered. As a result, residents established the Contra hatch into larvae. Mosquito Costa Mosquito Abatement District which began egg rafts are the size of a grain service in 1927. of rice. Today, the Contra Costa Mosquito and Vector LARVA Control District continues to protect public health The larva or ÒwigglerÓ comes with environmentally sound techniques, reliable and to the surface to breathe efficient services, as well as programs to combat Contra Costa County is home to 23 species of through a tube called a emerging diseases, all while preserving and/or mosquitoes. There are also several types of insects siphon and feeds on bacteria enhancing the environment.
    [Show full text]
  • Horse Insect Control Guide
    G950 (Revised March 2006) Horse Insect Control Guide John B. Campbell, Extension Entomologist feeds on blood. The fly bites inflict pain to the animal which Insects that bother horses, and ways to treat responds by foot stamping and tail switching in an effort to them, are covered here. dislodge the fly. House flies have a sponging type mouthpart and feed only on secretions of the animal around the eyes, nostrils and Nebraskans keep horses for a number of different rea­ anal openings. They are annoying to the animal even though sons. Some are for 4-H projects and urban users (recreation­ they don’t bite. al), ranch and farm (work), breeding farms, and racing. Both these fly species can transmit a nematode parasite Some of the insect pests of horses are also pests of other (Habronema spp.) to horses. The nematode is transmitted livestock. Other insects are specific to horses, but may be either through a feeding wound, or internally if the horse pests only on farm and ranch horses. swallows a fly. The best methods of pest control vary depending upon The nematode tunnels through the skin (cutaneous the type of horse production. tissues) of the horse, causing ulcerative sores (habroneiniasis or summer sores). The sores begin as small papules which Caution become encrusted. They are most often found on the shoulders, chest, neck, and inner surfaces of the rear quarters Use only insecticides that are USDA approved and EPA and tail. registered for use on horses. Wettable powder (WP) formula­ Localized treatment with a phosphate insecticide labeled tions are generally preferred over emulsifiable-concentrates for use on horses usually destroys the nematode.
    [Show full text]
  • Chironomid Midge and Mosquito Risk Assessment Guide for Constructed Water Bodies
    Chironomid midge and mosquito risk assessment guide for constructed water bodies Chironomid midge and mosquito risk assessment guide for constructed water bodies August 2007 Chironomid midge and mosquito risk assessment guide for constructed water bodies Acknowledgments This document has been developed by representatives from the Midge Research Group of Western Australia, including people with expertise in midge and mosquito management, as well as water body design and maintenance. In particular the contribution of the following people is gratefully acknowledged. Neil Harries (City of Gosnells) Sue Harrington (Department of Health) Dr Jenny Davis (Murdoch University) Ian Barker (formerly City of Rockingham) Paddy Strano (formerly City of Cockburn) Peter Morrison (formerly City of Canning) Daniel Rajah (City of Stirling) James Henson (City of Rockingham) This document is continually being reviewed and as such we welcome your feedback. Comments can be sent to the City of Cockburn at [email protected] Additional copies of this document can be downloaded from http://www.cockburn.wa.gov.au/midges/index.html Foreword This risk assessment guide has been developed to provide assistance to Approving Agencies, Developers and Landscape Designers in assessing design characteristics of proposed and existing Constructed Water Bodies. This document has been endorsed by the Department of Water, Department of Health and the Water Corporation. It is intended to provide a balance to minimising the potential for midge and mosquito breeding whilst at the same time endeavouring to allow flexibility in design and construction options. The guide provides a risk rating to various design parameters and users should select the most appropriate description of the proposed water body.
    [Show full text]
  • Infectivity of Housefly, Musca Domestica
    b r a z i l i a n j o u r n a l o f m i c r o b i o l o g y 4 7 (2 0 1 6) 807–816 ht tp://www.bjmicrobiol.com.br/ Environmental Microbiology Infectivity of housefly, Musca domestica (Diptera: Muscidae) to different entomopathogenic fungi ∗ Muzammil Farooq, Shoaib Freed Bahauddin Zakariya University, Faculty of Agricultural Sciences and Technology, Laboratory of Insect Microbiology and Biotechnology, Multan, Punjab, Pakistan a r t i c l e i n f o a b s t r a c t Article history: The housefly Musca domestica is a worldwide insect pest that acts as a vector for many Received 26 February 2014 pathogenic diseases in both people and animals. The present study was conducted to eval- Accepted 4 March 2016 uate the virulence of different local isolates of Beauveria bassiana, Metarhizium anisopliae and Available online 4 July 2016 Isaria fumosorosea on M. domestica using two bioassay techniques: (1) adult immersion and Associate Editor: Carlos Pelleschi (2) a bait method applied to both larvae and adults. The results showed evidence of a broad Taborda range of responses by both stages (larvae and adults) to the tested isolates of B. bassiana, M. anisopliae and I. fumosorosea. These responses were concentration-dependent, with mor- Keywords: tality percentages ranging from 53.00% to 96.00%. Because it resulted in lower LC50 values and a shorter lethal time, B. bassiana (Bb-01) proved to be the most virulent isolate against Entomopathogenic fungi Fecundity both housefly larvae and adults.
    [Show full text]
  • Diptera: Muscidae) Due to Habronema Muscae (Nematoda: Habronematidae
    ©2017 Institute of Parasitology, SAS, Košice DOI 10.1515/helm-2017-0029 HELMINTHOLOGIA, 54, 3: 225 – 230, 2017 Preimaginal mortality of Musca domestica (Diptera: Muscidae) due to Habronema muscae (Nematoda: Habronematidae) R. K. SCHUSTER Central Veterinary Research Laboratory, PO Box 597, Dubai, United Arab Emirates, E-mail: [email protected] Article info Summary Received December 29, 2016 In order to study the damage of Habronema muscae (Carter, 1861) on its intermediate host, Mus- Accepted April 24, 2017 ca domestica Linnaeus, 1758, fl y larval feeding experiments were carried out. For this, a defi ned number of praeimaginal stages of M. domestica was transferred in daily intervals (from day 0 to day 10) on faecal samples of a naturally infected horse harboring 269 adult H. muscae in its stomach. The development of M. domestica was monitored until imagines appeared. Harvested pupae were measured and weighted and the success of infection was studied by counting 3rd stage nematode larvae in freshly hatched fl ies. In addition, time of pupation and duration of the whole development of the fl ies was noticed. Pupation, hatching and preimaginal mortality rates were calculated and the number of nematode larvae in freshly hatched fl ies was counted. Adult fl ies harboured up to 60 Habronema larvae. Lower pupal volumes and weights, lower pupation rates and higher preimaginal mortality rates were found in experimental groups with long exposure to parasite eggs compared to experimental groups with short exposure or to the uninfected control groups. Maggots of the former groups pupated earlier and fl y imagines occurred earlier. These fi ndings clearly showed a negative impact of H.
    [Show full text]
  • A Systematic Review of Human Pathogens Carried by the Housefly
    Khamesipour et al. BMC Public Health (2018) 18:1049 https://doi.org/10.1186/s12889-018-5934-3 REVIEWARTICLE Open Access A systematic review of human pathogens carried by the housefly (Musca domestica L.) Faham Khamesipour1,2* , Kamran Bagheri Lankarani1, Behnam Honarvar1 and Tebit Emmanuel Kwenti3,4 Abstract Background: The synanthropic house fly, Musca domestica (Diptera: Muscidae), is a mechanical vector of pathogens (bacteria, fungi, viruses, and parasites), some of which cause serious diseases in humans and domestic animals. In the present study, a systematic review was done on the types and prevalence of human pathogens carried by the house fly. Methods: Major health-related electronic databases including PubMed, PubMed Central, Google Scholar, and Science Direct were searched (Last update 31/11/2017) for relevant literature on pathogens that have been isolated from the house fly. Results: Of the 1718 titles produced by bibliographic search, 99 were included in the review. Among the titles included, 69, 15, 3, 4, 1 and 7 described bacterial, fungi, bacteria+fungi, parasites, parasite+bacteria, and viral pathogens, respectively. Most of the house flies were captured in/around human habitation and animal farms. Pathogens were frequently isolated from body surfaces of the flies. Over 130 pathogens, predominantly bacteria (including some serious and life-threatening species) were identified from the house flies. Numerous publications also reported antimicrobial resistant bacteria and fungi isolated from house flies. Conclusions: This review showed that house flies carry a large number of pathogens which can cause serious infections in humans and animals. More studies are needed to identify new pathogens carried by the house fly.
    [Show full text]
  • The Crane Fly Vs. the Mosquito! Mosquito Crane
    The Crane Fly vs. The Mosquito! A Case of Mistaken Identity: A Crane Fly is not a Giant Mosquito! Mosquito Crane Fly It looks like a super-sized mosquito and it’s flying around your living room. Fatally attracted to light, these huge insects may cause panic upon first sight. Despite appearances, however, this seemingly dangerous insect is most likely a harmless common crane fly and not a mosquito at all. Common crane flies appear to be enlarged versions of many mosquito species, but there are several ways to tell them apart. The easiest way is by size. A mosquito is extremely small, measuring about ¼ - ½ inches in length. The common crane fly is between 1 – 1 ½ inches by comparison. Some crane fly species can even reach up to three inches! Another difference is that the crane fly will have a slender, V-shaped abdomen with long legs. This body shape makes crane flies poor fliers, and they usually wobble in the air. Mosquitoes, on the other hand, are agile and move quickly when flying. It is important to differentiate between these two bugs because mosquitoes transmit diseases like West Nile virus, encephalitis and Malaria, killing millions of people worldwide each year. Crane flies cannot bite and they do not carry diseases. As larvae, they may consume roots and vegetation while they are growing, but this is the extent of the damage they cause. In correctly called ‘mosquito eaters’ or ‘mosquito hawks’, crane flies actually feed on nectar or nothing at all in adult form. A crane fly’s sole purpose as an adult is to mate and die.
    [Show full text]
  • West Nile Virus (WNV) Fact Sheet
    West Nile Virus (WNV) Fact Sheet What Is West Nile Virus? How Does West Nile Virus Spread? West Nile virus infection can cause serious disease. WNV is ▪ Infected Mosquitoes. established as a seasonal epidemic in North America that WNV is spread by the bite of an infected mosquito. flares up in the summer and continues into the fall. This Mosquitoes become infected when they feed on fact sheet contains important information that can help infected birds. Infected mosquitoes can then spread you recognize and prevent West Nile virus. WNV to humans and other animals when they bite. What Can I Do to Prevent WNV? ▪ Transfusions, Transplants, and Mother-to-Child. In a very small number of cases, WNV also has been The easiest and best way to avoid WNV is to prevent spread directly from an infected person through blood mosquito bites. transfusions, organ transplants, breastfeeding and ▪ When outdoors, use repellents containing DEET, during pregnancy from mother to baby. picaridin, IR3535, some oil of lemon eucalyptus or para- Not through touching. menthane-diol. Follow the directions on the package. ▪ WNV is not spread through casual contact such as ▪ Many mosquitoes are most active from dusk to dawn. touching or kissing a person with the virus. Be sure to use insect repellent and wear long sleeves and pants at these times or consider staying indoors How Soon Do Infected People Get Sick? during these hours. People typically develop symptoms between 3 and 14 days after they are bitten by the infected mosquito. ▪ Make sure you have good screens on your windows and doors to keep mosquitoes out.
    [Show full text]
  • Ophthalmic and Cutaneous
    ISRAEL JOURNAL OF VETERINARY MEDICINE OPHTHALMIC AND CUTANEOUS HABRONEMIASIS IN A HORSE: CASE REPORT AND REVIEW OF THE LITERATURE Yarmut Y., Brommer H., Weisler S., Shelah M., Komarovsky O., and Steinman A*. a Koret School of Veterinary Medicine, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel. b Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University. Yalelaan 114, NL-3584 CM, Utrecht, The Netherlands. c Kfar Shmuel 13, 99788, Israel. * Corresponding author. A. Steinman Tel.: +972-54-8820-516; Fax: +972-3-9604-079. E-mail address: [email protected] Hospital (KSVM-VTH). The horse presented skin lesions around INTRODUCTION the medial canthus of the right eye and on the lateral bulb of Habronemiasis is a parasitic disease of equids (horses, donkeysth,e heel of the right front leg. The lesions were first noticed 3 mules and zebras) caused by the nematodes Habronema musca,week s previously and the referring veterinarian had suspected H. majus andDraschia microstoma (1,2). The adult worms livhabronemiasise . The horse was treated with ivermectin 1.87 % on the wall of the stomach of the host without internal migrationper .os (Eqvalan Veterinary® 200 ug/kg, Merial B.V., Haarlem, Embryonated eggs are excreted in the feces to the environmenNetherlands)t , and dexamethasone intramuscularly (Dexacort where they are ingested by the larvae of intermediate hosts, sucForte®h , 20 mg/ml Teva Pharmaceut. Works Private Ltd. Co, as houseflies and stable flies. Most cases of gastric habronemiasiHungary)s , twice every second day.
    [Show full text]
  • June 2019 ETBA Newsletter
    East Texas Beekeepers Association June 6, 2019 June Report by Dick Counts Beekeepers should be periodically checking their hives for capped honey. You do not want to run out of space for your bees to store honey during the flow. If your super has eight frames of honey, add another super. It is time to start thinking about extracting. Soon, I will be setting up my extraction equipment in preparation for club extraction days. ETBA members are invited to use my equipment to extract their honey if they do not own or have access to another extractor. Extraction days can get pretty busy, so we have a few rules to make the process work smoothly. First you must be an ETBA member. Secondly, you must make an appointment and also tell me how many supers you will be bringing to extract. Please arrive about 15 minutes before your appointment time. My yard has limited space for parking so we try to not to have a large number of members arriving at the same time. Be aware that there will be a lot of bees flying all through the yard, so do not bring pets or unattended children. Bring a clean, dry wide-mouth container with a lid to collect your honey from the extractor. A food grade five gallon bucket works well. You can figure on two supers per bucket. You will fill your individual honey jars at home. Finally, get the bees off your supers before you arrive. Also have something to cover them or my bees will find them.
    [Show full text]
  • Original Papers Molecular Characterization of the First Internal
    Annals of Parasitology 2015, 61(4), 241-246 Copyright© 2015 Polish Parasitological Society doi: 10.17420/ap6104.13 Original papers Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep Seyed Sajjad Hasheminasab Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Qareeb St., Azadi Ave., 1419963111 Tehran, Iran; E-mail: [email protected] ABSTRACT. Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of Parabronema skrjabini were amplified by polymerase chain reaction (PCR) using two pairs of specific primers (Para-Ir-R and Para-Ir-F). ITS1 homology in the sequence of this study was 69% compared with the sequence data in GenBank. To our knowledge, this is the first study in the world exploring the genetic diversity of P. skrjabini in sheep based on ITS1. Key words: Parabronema skrjabini , PCR, Sanandaj, Iran Introduction candidate [15]. A range of studies has demonstrated that polymerase chain reaction (PCR)-based Parabronema skrjabini is one of the nematodes approaches can be used for the species specific that occurs in the abomasum of ruminants and has a identification of parasitic nematodes (from different wide distribution in Africa, Asia and some orders), irrespective of developmental stage [16]. P. Mediterranean countries.
    [Show full text]